ライフサイエンスコーパス: specific primer

1 a nested, gene-specific primer and a linker-specific primer.

2 on of each message complementary to each ORF-specific primer.

3 generated with mycoplasma open reading frame-specific primers.

4 f Nocardia species was amplified using secA1-specific primers.

5 tative real-time RT-PCR with bacterial group-specific primers.

6 d by polymerase chain reaction with sequence-specific primers.

7 rs were examined by genotyping with sequence-specific primers.

8 testing of potentially millions of amplicon-specific primers.

9 mplification of the genomic DNA using vector-specific primers.

10 appropriate V(beta)- and V(alpha)-subfamily-specific primers.

11 jected to reverse transcription-PCR with SPV-specific primers.

12 plified from serum by a PCR using coccidioid-specific primers.

13 dominant clones and used to design clonotype-specific primers.

14 titative PCR on the same RNA pair using gene-specific primers.

15 sured by competitive PCR of cDNA using locus-specific primers.

16 and PCR using genus-specific and H. cetorum-specific primers.

17 by polymerase chain reaction using sequence-specific primers.

18 ned using real-time quantitative RT-PCR with specific primers.

19 reactions at actual melting temperatures of specific primers.

20 s were positive for H. cetorum using species-specific primers.

21 was used as a template for PCR with JSRV gag-specific primers.

22 The retinas were evaluated by PCR with Y-specific primers.

23 everse transcription-PCR analysis using gene-specific primers.

24 by polymerase chain reaction using sequence-specific primers.

25 se chain reaction amplification with library-specific primers.

26 escently-labeled energy-transfer (ET) allele-specific primers.

27 ant mRNA in the individual tumor cells using specific primers.

28 the polymerase chain reaction with sequence specific primers.

29 PCR and GABA(A) beta(2) and beta(3) subunit-specific primers.

30 by the use of a PCR with Helicobacter genus-specific primers.

31 ols using polymerase chain reaction sequence specific primers.

32 rase chain reaction using group M-, O-, or N-specific primers.

33 ormal rat cholangiocyte cell line using mdr1-specific primers.

34 The MTHFR gene was amplified using specific primers.

35 CR products and cloned genomic DNA with cDNA-specific primers.

36 to the 3' ends of the EagI-, SmaI-, or XbaI-specific primers.

37 odification and primer extension with allele-specific primers.

38 cation of closed circular DNA using sequence-specific primers.

39 lification was obtained with Dehalococcoides-specific primers.

40 tive reverse-transcription PCR using isoform-specific primers.

41 by polymerase chain reaction using sequence-specific primers.

42 quencing of the ITS rDNA region using fungal-specific primers.

43 olymerase chain reaction (PCR) with sequence-specific primers.

44 esulting chimeras were analyzed with species-specific primers.

45 l transcripts were identified by RT-PCR with specific primers.

46 Viruses were subtyped using subtype-specific primers.

47 alleviate the difficulty in designing target-specific primers.

48 esidue and fluorescent reporters on genotype-specific primers.

49 bedded tissue and amplified by using species-specific primers.

50 by quantitative real-time PCR using species-specific primers.

51 mental procedures using glia and neuron gene specific primers.

52 romotion assay and a PCR analysis using cfrA-specific primers.

53 fficient and reliable for designing sequence-specific primers.

54 ribosomal RNA gene from feces with T. foetus-specific primers (34 out of 36).

55 er PCR (MPN-PCR) and five distinct bont gene-specific primers (A, B, C, E, and F).

56 RT-PCR analyses using gene-specific primers allowed us to monitor the expression of

57 The allele-specific primers also carried complementary stem structu

58 lleles by polymerase chain reaction-sequence-specific primer amplification and categorized the number

59 The MycoAlign system uses pan-Mycobacterium-specific primer amplification in combination with a cust

60 We designed 19 new specific primers anchored in a stepwise fashion in order

61 NA ligase; and (iv) PCR using a nested, gene-specific primer and a linker-specific primer.

62 transcriptase PCR (RT-PCR) using the vector-specific primer and followed by direct sequencing.

63 mmetric PCR is performed using a single gene-specific primer and standard dNTPs.

64 Subsequent PCR using a nested gene-specific primer and the 3' or 5' T-RACE primer results i

65 sing both Plasmodium genus and P. falciparum-specific primers and 5.8 parasites/microl for P. ovale,

66 Using species-specific primers and a fluorescent probe, detection of D

67 For this purpose, specific primers and a minor groove binding (MGB) TaqMan

68 A system of specific primers and a Minor Groove Binding (MGB) TaqMan

69 western blot, and immunohistochemistry with specific primers and a well-characterized antibody to th

70 DNA sequencing with methylation-specific primers and cDNA analysis in patient neurons in

71 same liver were analyzed by PCR using Vbeta-specific primers and CDR3 analysis.

72 -treated PBLs of seven donors using provirus-specific primers and corroborated the results with a sub

73 ublished in GenBank and Ensembl, we designed specific primers and detected by PCR three mRNA species

74 ds and yeasts by employing universal, fungus-specific primers and DNA probes in an enzyme immunoassay

75 HLA-specific primers and fluorogenic probes were used in rea

76 ection was performed with tcdA and tcdB gene-specific primers and hydrolysis probes using the LightCy

77 CR strategy that employs both unlabeled gene-specific primers and labeled universal primers, allows f

78 We used paralog-specific primers and long-range PCR to clone, sequence,

79 By use of variola virus-specific primers and long-range PCR, 22 overlapping ampl

80 ped using polymerase chain reaction sequence-specific primers and polymerase chain reaction followed

81 dPCR) for quantifying HRV RNA using genotype-specific primers and probes and a consensus primer/probe

82 lized Scorpion probes that combined genotype-specific primers and probes for the 18S rRNA gene into t

83 of these two different WT1 transcripts with specific primers and probes that ensure specificity for

84 quantitative polymerase chain reaction with specific primers and probes to detect bacterial DNA from

85 Group-specific primers and probes were developed to detect num

86 bridization assays with porcine NoV- and SaV-specific primers and probes, respectively.

87 By using the appropriate TCRbeta CDR3-specific primers and probes, the GVH- and GVL-specific c

88 sis using species-, virulence-, and serotype-specific primers and probes.

89 described species-, virulence-, and serotype-specific primers and probes.

90 Gene-specific primers and reverse transcriptase were used to

91 By using specific primers and reverse transcription-polymerase ch

92 idopsis, a family-wide survey involving gene-specific primers and RT-PCR was conducted.

93 by polymerase chain reaction, using sequence-specific primers and sequence-specific oligonucleotide p

94 Using gene-specific primers and specific antibodies, expression of

95 f urinary cell BKV VP1 mRNA levels using BKV specific primers and TaqMan probe in a real-time quantit

96 um DNA barcode library, and designed species-specific primers and TaqMan probes for the above six Tri

97 hain reaction (RT-PCR) with indexed genotype-specific primers and the same products were sequenced us

98 polymerase chain reaction (RT-PCR) with gene-specific primers, and cDNA sequencing analyses we determ

99 stem-polymerase chain reaction with sequence-specific primers, and Cox proportional hazards models we

100 luding single-base extension primers, allele-specific primers, and tetra-primers for tetra-primer ARM

101 DNA template is unwound by the T7 helicase, specific primers anneal to the separated DNA strands and

102 A molecules, as well as sequestration of non-specific primer annealing templates into negative chambe

103 However, specific primer approaches have limited the ability to f

104 Second, different sets of specific primers are immobilized within various gel pads

105 a competitive approach, whereby both allele-specific primers are present in the same reaction and ca

106 First, marker-specific primers are used in PCR amplifications of genom

107 Cs) with complementary sequences to CAM gene-specific primers as internal standards.

108 of these mRNAs was confirmed by RT-PCR with specific primers as well as by in situ hybridization.

109 ersus control tissue were assayed using gene-specific primers at times corresponding to premigratory

110 he genomic sequence, isolated with PCR using specific primers based on the cDNA sequence, does not co

111 ighly contaminated samples, we evaluated new specific primers based on the DNA base sequence within t

112 Ten pairs of species-specific primers based on the ITS2 rDNA were developed f

113 t al. (E1/E2) were incorporated with species-specific primers based upon the superoxide dismutase (so

114 od of about 1 year with an H. pylori species-specific primer-based PCR analysis of DNA extracted from

115 ing of a plasmid DNA molecule containing HBV-specific primer binding regions, and the sensitivity of

116 tified the bias of previously published gene-specific primers by comparing the repertoires generated

117 ed and amplified with NOS1-, NOS2-, and NOS3-specific primers by RT-PCR.

118 the immunoprecipitated products using CD154-specific primers clearly demonstrated that nucleolin and

119 highly representative cDNA library using ORF-specific primers, cloned by Gateway recombination clonin

120 ovarian ribonucleoprotein complex with gene-specific primers confirmed the association of LHR mRNA w

121 tivity estimation indicated that the species-specific primers could correctly amplify the target ITS2

122 Additionally, these species-specific primers could quantify specificity and identify

123 Use of these or other, less species-specific, primers could lead to a false-positive diagnos

124 rse transcriptase-PCR analysis using isoform-specific primers demonstrate that d2 is expressed mainly

125 ation of reverse-transcribed mRNA using gene-specific primers demonstrated expression of both cystic

126 scriptase PCR analysis of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are

127 mplification of the resultant cDNA with gene-specific primers demonstrated the presence in mitochondr

128 mic DNA from the SI-1 B cell line and HTLV-1-specific primers demonstrated the presence of a full-len

129 ctions with both vector-derived and receptor-specific primers demonstrated the presence of both corre

130 The gene-specific primers demonstrated the presence or absence of

131 Mycobacterium genus-specific primers derived from highly conserved sequences

132 purpose, we developed a new feature, homolog-specific primer design (HSPD), in our high-throughput pr

133 However, non-specific primer design can lead to false-positive detect

134 The sequence-specific primer design for DNA meth-ylated sequences inc

135 This highlights the critical need for specific primer design for MSP.

136 First, RT-PCR using sequence-specific primers detected the transcript in the stellate

137 In this study, Enterococcus genus-specific primers developed by Deasy et al. (E1/E2) were

138 Family-specific primers did not amplify genes from the other fa

139 rrently available set of 4,290 unique 3' ORF-specific primers do not hybridize to each ORF with equal

140 In the present study, specific primers enhanced detection of A. vaginae and pr

141 s of array probes are available for sequence-specific primer extension and ligation reactions.

142 -amplified DNA to arrayed probes with allele-specific primer extension and signal amplification.

143 9 ascomycetous yeast species, and the allele-specific primer extension assay was designed to identify

144 From culture to identification, the allele-specific primer extension method takes 8 h and the direc

145 Direct hybridization and allele-specific primer extension methods were both successful i

146 er that is activated to carry out the allele-specific primer extension reaction to detect mutations.

147 Preliminary analysis using gene-specific primers failed to amplify tprJ in the Sea 81-4

148 nts by polymerase chain reaction (PCR) using specific primers flanking the targeted genes.

149 ction (PCR) amplification of DNA with allele-specific primers followed by allele-specific restriction

150 reverse-transcribed mRNA using murine CYP2C-specific primers followed by cloning and sequencing iden

151 t the combination of RT using a pool of gene-specific primers followed by semi-nested PCR resulted in

152 n U373 cells as assessed by RT-PCR using CaR-specific primers followed by sequencing of the amplified

153 icks by reverse transcription-PCR using gene-specific primers followed by Southern blotting.

154 iques, including PCR amplification with gene-specific primers, followed by gel electrophoresis or mic

155 fication of each predicted ORF by using gene specific primers, followed by in vivo homologous recombi

156 Human tRNA(Lys,3) is the specific primer for HIV-1 reverse transcriptase and also

157 PCR with specific primers for 16S rRNA, 444 Ep-ank and groESL hea

158 tissue-derived cDNA and amplified with gene-specific primers for 19-26 PCR cycles.

159 d source of wild-caught L. longipalpis using specific primers for a locus from the chloroplast genome

160 We have designed sequence-specific primers for detection of the rhesus macaque MHC

161 transcription-PCR was carried out using gene-specific primers for each of the Arabidopsis annexin gen

162 The splice variants were characterized using specific primers for G(o)alpha1 and G(o)alpha2 in conjun

163 By use of 16S rRNA species-specific primers for H. marmotae, two additional liver s

164 PCR analysis using specific primers for IMP-1, L1, CcrA, and bla(B/C) confi

165 ted with the polymerase chain reaction using specific primers for platelet-derived growth factor (PDG

166 Species-specific primers for reverse transcription quantitative

167 a was cloned and used to generate midshipman-specific primers for RT and real-time PCR which identifi

168 sed by real-time quantitative PCR using gene-specific primers for selected (n=20) genes.

169 larity, making it difficult to design genome-specific primers for sequence analysis.

170 n having an amplicon size of 530bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3.

171 criptase polymerase chain reaction with gene-specific primers for six of the nine maize genes indicat

172 ase-polymerase chain reaction (RT-PCR) using specific primers for the CRP.

173 We developed specific primers for the cytochrome c oxidase subunit 1

174 notypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E7 genes.

175 We designed HRM-specific primers for the Mytilus genus to identify M. ch

176 and renal biopsy tissue was performed using specific primers for the transcription control regions o

177 t can be used to select highly sensitive and specific primers for virus subtyping.

178 hod comprises a PCR system with four sets of specific primers, for each target species.

179 n of mitochondrial D loop gene using species specific primers found 226bp and 126bp product amplicons

180 by a reverse transcription-PCR that employs specific primers from a member of the heat shock protein

181 GSP also designs specific primers from multiple sequences uploaded by use

182 Under optimal amplification conditions, glmM-specific primers generated PCR-amplified products that w

183 t genes from this subtraction and using gene-specific primers have confirmed the transcriptional upre

184 hod that combines oligo-(dT) with an 18S-RNA-specific primer in the initial reverse transcription (RT

185 ription-polymerase chain reaction with mouse specific primers in either CasR(+/+) or CasR(-/-) osteob

186 imer-BLAST allows users to design new target-specific primers in one step as well as to check the spe

187 esent study, we have used a pair of sequence-specific primers in reverse transcription-polymerase cha

188 everse transcriptase-PCR amplification using specific primers in two teleost fish, winter flounder (P

189 chain reaction in association with sequence-specific primers incorporating mismatches at the 3' end.

190 on analysis followed by PCR with RI promoter-specific primers indicated an accumulation of acetylated

191 nested-PCR approach using non-degenerate MIH-specific primers indicated the presence of MIH transcrip

192 homogeneous distribution of mutant p53 with specific primers, indicating that only subgroups of the

193 ning the recombinant was subjected to insert-specific primer-mediated PCR amplification using a high-

194 Amplification is performed using target-specific primers modified with a 5'-end tail that is com

195 riction enzyme and multiplexed PCR with gene-specific primers (MSRE-PCR).

196 Using 0.5 microg of total RNA and GC-C-specific primers, nested RT-PCR detected a single human

197 length circular mtDNA by either custom mtDNA-specific primers or a commercial kit, and minimizes the

198 eously in a complex DNA sample without locus-specific primers or automation.

199 tion melt (RT-PCR-HRM) assays without allele-specific primers or probes and post-PCR sample handling

200 Each isoform mRNA is quantified using a specific primer pair and a 5'FAM- and 3'TAMARA-labeled p

201 A variant-specific primer pair is required to amplify each alterna

202 PsASGR-BABY BOOM-like (psASGR-BBML)-specific primer pair p779/p780 was in perfect linkage wi

203 Using a specific primer pair to amplify Nanog by reverse transcr

204 ment was sequenced and then used to design a specific primer pair, Nf1 (16-mer) and Nf2 (16-mer), com

205 Quantitative PCR of stool DNA with species-specific primer pairs demonstrated significantly reduced

206 forms of Ang-1 was confirmed by RT-PCR using specific primer pairs derived from junction sites and th

207 developed that ensures the design of target-specific primer pairs for DNA amplification.

208 However, real-time PCR using gene-specific primer pairs only amplified Ee-BAM1, indicating

209 Baseline DNA extraction yielded 17 clone-specific primer pairs.

210 quantitative PCR (QPCR) analysis with genome-specific primer pairs.

211 single-stranded circular template DNA using specific primer pairs.

212 g the replication status of each virus using specific primer pairs.

213 Individualized tumor-specific primer panels of aberrant chromosomal junctions

214 parts of the country were genotyped by type specific primer PCR method.

215 tumor necrosis factor TNF typed by sequence-specific primer PCR, and the results compared to those f

216 (RTI) has been limited for the most part to specific primer PCR-based methods due to their increased

217 TGF-beta1 variants were analyzed by sequence-specific primer PCR.

218 PB1 genotypes were determined using sequence-specific primer PCR.

219 y of polymerase chain reaction with sequence-specific primers (PCR-SSP) typing for HLA class II allel

220 d by polymerase chain reaction with sequence-specific primers (PCR-SSP).

221 multiplex polymerase chain reaction-sequence-specific primers (PCR-SSPs).

222 -DQB1 genotypes were determined by (sequence-specific primers) PCR.

223 target amplification with only one sequence-specific primer per target and a second primer that is c

224 rphism (rs1042522) was analyzed using allele-specific primer polymerase chain reaction.

225 ms in EDN1, EDNRA, and EDNRB, using sequence-specific primer-polymerase chain reaction.

226 By sequence-specific primers-polymerase chain reaction (SSP-PCR) fiv

227 among different deer species, a fallow deer-specific primer/probe system targeting a fragment of the

228 psies from all 10 subjects, RT-PCR with RAGE-specific primers produced a band of the predicted size.

229 Whereas the glmM-specific primers provide a rapid, sensitive presumptive

230 The PCR uses six sets of ampC-specific primers resulting in amplicons that range from

231 dy of African-American neonates using allele-specific primers revealed a statistically significant as

232 PCR analysis using E. canis-specific primers revealed that 17 of the 55 dog blood sa

233 peripheral blood cells of RA patients using specific primer sequences for five key microsatellites.

234 arasites/microl for P. vivax using the genus specific primer set.

235 ranscriptase polymerase chain reaction using specific primer sets.

236 Possibly, specific primers sets can be identified based on the pre

237 RT-PCR with zGCAP specific primers showed specific expression of zGCAPs an

238 RT-PCR using CLCA-specific primers showed the expression of CLCA1 in both

239 be more sensitive than the published species-specific primers specifically developed for the LAMP met

240 Four modified sequence specific primers (SSP) pairs were designed for the selec

241 g the polymerase chain reaction and sequence-specific primers (SSP-PCRs) and of the CTLA-4 and TNF re

242 he HIP element, alone or in combination with specific primer subsets, for analyzing differential gene

243 ing of individual clones with insertion-site-specific primers suggested that clones contributing to h

244 e dimer, pppAC, the initial step of sequence-specific primer synthesis, is not sufficient for the sta

245 Application of previously published genus-specific primers targeting 16S rRNA gene sequences revea

246 y an important role in recognizing their own specific primer-template RNA structure for the cDNA prim

247 is), pork (Sus scrofa), and cow (Bos taurus) specific primers that amplify fragments (horse; 85 bp, s

248 ine (Bos taurus) and poultry (Gallus gallus) specific primers that amplify small fragments (amplicons

249 particular gene, PCR is performed with gene-specific primers that are end-labeled with fluorescent m

250 red interval is PCR-amplified using sequence-specific primers that are flanked by the requisite targe

251 t GSP, a web-based platform to design genome-specific primers that distinguish subgenome sequences in

252 The effect of TNF was studied using isoform-specific primers that flanked unique regions of two majo

253 studied were identified by RT-PCR using cell-specific primers that included Myh11 (smooth muscle cell

254 cation of genomic DNA with two tailed allele-specific primers that introduce priming sites for univer

255 reen technology (LSG-qPCR), which uses genus-specific primers that target the ITS1 region and amplify

256 Using real-time RT-PCR with specific primers, the mRNA transcripts in rat tibialis a

257 ariant linker sequences and modular template-specific primers to allow for the simultaneous generatio

258 ased method that uses a matrix of BV- and BJ-specific primers to amplify 240 distinct BV-BJ combinati

259 developed a multiplex PCR that uses species-specific primers to amplify gene regions in four species

260 The method utilizes specific primers to amplify target transgenes, and endog

261 hain reaction (PCR) with degenerate and gene-specific primers to clone part of the secreted form of s

262 We used pathogen-specific primers to detect DNA from JC virus (JCV), vari

263 mplemented by amplicon-based sequencing with specific primers to ensure 100% coverage of all targeted

264 olymerase chain reaction with multiple group-specific primers to evaluate the impact of antibiotic tr

265 Using gene-specific primers to explore the genomic organization of

266 ail is attached to one of the forward allele-specific primers to increase the Tm of the amplification

267 rimer as a walker primer and a set of nested specific primers to perform two to three successive roun

268 rase chain reaction was performed using Muc4-specific primers to quantitate Muc4 mRNA expression in o

269 as used, which does not require partner gene-specific primers, to identify the chimeric transcript.

270 The process of designing specific primers typically involves two stages.

271 rse transcription-PCR performed with rat VR1-specific primers verified the expression of VR1 mRNA in

272 known origin, conventional PCR using species-specific primers was carried out on the extracted DNA.

273 A pair of strain-specific primers was designed based on the 16S rRNA gene

274 ar orientalis strain CO92, a set of 27 locus-specific primers was designed to amplify fragments betwe

275 ng calcium-activated chloride channel (CLCA)-specific primers was used to examine the expression of C

276 and quantitative real-time PCR with species-specific primers was used to quantify the biomass in peg

277 Polymerase chain reaction using sequence-specific primers was utilized to ascertain the prevalenc

278 -PCR and a combination of generic and strain-specific primers, we amplified 13 subgenomic fragments w

279 Using RT-PCR with env-specific primers, we could detect expression of PERV cla

280 Using allele-specific primers, we found 13.8% of all samples (n = 164

281 Using species-specific primers, we found a significant increase in MMP

282 Specific primers were designed and used to examine the m

283 UCP-subtype-specific primers were designed for the assay.

284 Species-specific primers were designed from cytochrome b, cytoch

285 Five sets of species-specific primers were designed from the internal transcr

286 Five pairs of species-specific primers were designed targeting mitochondrial N

287 ragments generated by PCR with P. gingivalis-specific primers were hybridized to fragments from refer

288 r assays, and semiquantitative PCR with exon-specific primers were performed using human-derived RPE

289 To achieve this, six species-specific primers were selected from previous reports and

290 Additionally, the species-specific primers were tested against a panel of 200 clin

291 ostic lymphoblasts and sequenced and patient-specific primers were used to amplify DNA from blood sam

292 BAC fingerprinting and RGA-specific primers were used to develop a contig of BAC cl

293 Additionally, fish-specific primers were used to sequence the forensically

294 The C. coli-specific primers were validated with 53 isolates from hu

295 was performed as well by PCR using sequence-specific primers, whereas cytokine production was evalua

296 -round symmetric PCR is performed with genus-specific primers which selectively target and amplify a

297 discrimination was carried out using allele-specific primers, which flanked the point mutation in th

298 ion protocol that combines nested, insertion-specific primers with degenerate primers, to amplify DNA

299 ts of betaA3-crystallin were generated using specific primers with deletions of N-terminal extension

300 Using genus-specific primers with qPCR, we observed an increase in t