ライフサイエンスコーパス: spermatid

1 zed within the two huge mitochondria of each spermatid.

2 the activation signal is transduced into the spermatid.

3 erentiating spermatogonia, spermatocytes and spermatids.

4 eiotic spermatocytes and postmeiotic haploid spermatids.

5 NHE8 localizes to the developing acrosome of spermatids.

6 rom inactive sex chromosomes in post-meiotic spermatids.

7 ls but was undetectable in spermatocytes and spermatids.

8 ganization property of Brdt in haploid round spermatids.

9 colocalizes with acetylated H4 in elongating spermatids.

10 are unable to mature into spermatocytes and spermatids.

11 s silencing and is activated in post-meiotic spermatids.

12 meiosis I and after exit from meiosis II, in spermatids.

13 is, and silencing persists into post-meiotic spermatids.

14 Dicer-null pachytene spermatocytes or round spermatids.

15 branous organelles (MOs) in undifferentiated spermatids.

16 control versus Brdt(DeltaBD1/DeltaBD1) round spermatids.

17 '-untranslated region (UTR) typical of round spermatids.

18 ished on the sex chromosomes in post-meiotic spermatids.

19 rise to bi-nucleated or even tetra-nucleated spermatids.

20 in investigating sex chromosome dynamics in spermatids.

21 and Mena in the subacrosomal layer of round spermatids.

22 ndergo chromatin reorganization in elongated spermatids.

23 icing and localizes to giant mitochondria in spermatids.

24 s to repress sex chromosome transcription in spermatids.

25 ion and maintain steady expression levels in spermatids.

26 d primarily in testis, almost exclusively in spermatids.

27 SLY is found in the nucleus and cytoplasm of spermatids.

28 ern of ACROSIN staining as observed in human spermatids.

29 g to substantial spermidine synthesis in the spermatids.

30 of meiosis as noted by the presence of round spermatids.

31 are essential for chromatin condensation in spermatids.

32 n the X chromosome remain repressed in round spermatids.

33 ely created from mRNAs that are expressed in spermatids.

34 e expression in haploid spermatogenic cells, spermatids.

35 lt pachytene spermatocytes, as well as round spermatids.

36 whose mRNAs are most abundant in elongating spermatids.

37 ze to the nucleus of either spermatocytes or spermatids.

38 hytene and diplotene spermatocytes and early spermatids.

39 oietic parameters, and a decreased number of spermatids.

40 the testis, particularly elongating/elongate spermatids.

41 ion sequesters RNF8 in the cytoplasm of late spermatids.

42 a centriole-independent compartment in mouse spermatids.

43 g in a decreased number of spermatocytes and spermatids.

44 from the primary spermatocyte stage through spermatids.

45 al region of round, elongating and elongated spermatids.

46 anscriptionally inactive state in late-stage spermatids.

47 he onset of MSCI and persists in postmeiotic spermatids.

48 marked decrease of the endogenous kinases in spermatids.

49 Here we report that, in Drosophila spermatids, a conserved module of transition zone protei

50 dramatic Se enrichment specifically in late spermatids, a pattern that was not seen in any other ele

51 m and hypomorphic phenotypes being premature spermatid activation and numerous sperm cell defects.

52 appears to be involved in the regulation of spermatid activation during spermiogenesis, with the nul

53 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in

54 d production by meiosis) and spermiogenesis (spermatid activation into actively motile spermatozoa).

55 product functions in the transduction of the spermatid activation signal.

56 ould perturb spermatogenesis, in particular, spermatid adhesion (i.e., inducing apical ES degeneratio

57 tly regulated in adult rat testis to control spermatid adhesion and sperm release at spermiation.

58 hat sFRP1 might be correlated with elongated spermatid adhesion conferred by the apical ES before spe

59 alizes to cytoplasmic structures of maturing spermatids affected in Kdm3a mutant mice, which in turn

60 tis, the CRB3 KD testes displayed defects in spermatid and phagosome transport, and also spermatid po

61 Zona pellucida binding protein 1 (ZPBP1), a spermatid and spermatozoon protein that localizes to the

62 rdt protein was lost in Brdt(BD1/BD1) mutant spermatids and Brdt and Sirt1 overlapped around the chro

63 s, hMCA localized to the tails of developing spermatids and did not localize to the nucleus of either

64 stochemistry also in embryonic gonads and in spermatids and granulosa cells of adult testes and ovari

65 32 kDa murine JAM-A is present in elongated spermatids and in the plasma membrane of the head and fl

66 acrosomal formation, degenerative elongating spermatids and irregular head morphology in postmeiotic

67 The Bsg KO testis lacks elongated spermatids and mature spermatozoa, a phenotype similar t

68 concomitant with defects in the transport of spermatids and phagosomes and a disruption of cell adhes

69 that they appear in elongating to condensing spermatids and predominantly associated with the chromat

70 and Hnrnpk mRNAs were longer in mutant round spermatids and resulted in reduced protein levels.

71 All three isozymes are present only in spermatids and sperm and have distinctive features that

72 testis and more specifically in postmeiotic spermatids and sperm cells.

73 ubstrate pair localized to the centrioles of spermatids and spermatozoa.

74 ntially become spermatogonia, spermatocytes, spermatids, and eventually spermatozoa.

75 1 of mouse spermatogenesis in the elongating spermatids, and it subsequently incorporates into the fl

76 apical ectoplasmic specialization, malformed spermatids, and marked hyperspermia.

77 d spermatids, apoptosis of spermatocytes and spermatids, and the appearance of numerous round cells i

78 n RNPs from pachytene spermatocytes to round spermatids, and the enrichment of shorter 3' UTR mRNAs i

79 esis was first detected in step 9 elongating spermatids, and those elongated spermatids that were for

80 cal alterations during acrosome formation in spermatids, and were significantly different in connecti

81 Tnp1, Spem1, and Catpser3; depletion of late spermatids; and male infertility.

82 ES is restricted to the Sertoli-spermatid (apical ES) interface, as well as the Sertoli

83 chimeras included failure to form elongated spermatids, apoptosis of spermatocytes and spermatids, a

84 progressively repressed as nuclei of haploid spermatids are compacted through a dramatic chromatin re

85 A high proportion of delayed spermatids are eliminated via apoptosis, with a conseque

86 bule where secondary spermatocytes and early spermatids are found, suggesting a role for D930015E06Ri

87 These translation defects in haploid round spermatids are likely indirect, as neither MAEL nor piRN

88 sh with semicystic spermatogenesis, in which spermatids are released into the seminiferous lobule lum

89 oward the apical compartment until elongated spermatids are released into the tubule lumen during spe

90 family of post-meiotic kinases expressed in spermatids, are critical to spermiogenesis, and are requ

91 of BRD4 with acetylated H4 decreases in late spermatids as acetylated histones are removed from the c

92 s co-localized with SSTK in the cytoplasm of spermatids as they undergo restructuring and chromatin c

93 NA interference created XX animals that made spermatids as well as oocytes, but their spermatids coul

94 Pygo2 is expressed in elongating spermatids at stages when chromatin remodeling occurs, a

95 taining the Gfp coding region in early round spermatids at the same transcription start site as the n

96 required for proper basal body function and spermatid axoneme formation.

97 m functional centrosomes, but centrioles and spermatid basal bodies are short in length.

98 entriolar protein that also localizes to the spermatid basal body.

99 homeodomain transcription factor 1 and round spermatid basic protein 1.

100 Their round spermatids bear "ghost" CBs, whose architecture is great

101 satellite repeats but not drive-insensitive spermatids bearing few or no Rsp satellite repeats.

102 e-to-protamine transition in drive-sensitive spermatids bearing many Rsp satellite repeats but not dr

103 Once spermatids become activated to spermatozoa, the reporter

104 y disappeared at the apical ES in misaligned spermatids before their depletion.

105 5 is discarded into the residual body during spermatid budding, but a small amount remains in budded

106 l region at the distal elongating end of the spermatid bundles, thus they represent a new class of su

107 xpressed in differentiated spermatocytes and spermatids but not in undifferentiated spermatogonia, st

108 ype mice, and in the manchette of elongating spermatids, but in the Meig1 or Pacrg-deficient mice, SP

109 tants, persisting nucleoids are swept out of spermatids by a cellular remodeling process that trims a

110 the reorganization of hyperacetylated round spermatid chromatin.

111 In elongating spermatids, CnnT forms speckles on the giant mitochondri

112 lasm is incomplete in Ube2j1(-/-) elongating spermatids, compromising the release of mature elongate

113 CAPZA3 protein localization was altered in spermatids concurrent with altered localization of a uni

114 revealed BRD4 in a ring around the nuclei of spermatids containing hyperacetylated histones.

115 ade spermatids as well as oocytes, but their spermatids could not activate without the addition of ma

116 P90 and TSSKs in germ cells, a mouse primary spermatid culture model was developed and characterized.

117 cts the metabolic processes occurring in the spermatid cytoplasm but does not lead to a global pertur

118 e variable effects, from dramatic muscle and spermatid defects in Drosophila to more subtle neurophys

119 ermatogenic and Sertoli cells as well as the spermatid deformities.

120 By comparing the development of sperm- and spermatid-derived frog embryos, we show that the program

121 of Utp14b transcripts were highest in round spermatids despite the transcription of Utp14a in these

122 The Rfx2-null round spermatids detached from the seminiferous tubules, formi

123 ls (LCs) and germ cells and is essential for spermatid development and completion of spermatogenesis.

124 y role as a morphogenetic determinant during spermatid development in the water fern Marsilea vestita

125 mal in the absence of GLD2, but post-meiotic spermatid development rapidly becomes abnormal.

126 es an immune-privileged site for postmeiotic spermatid development to avoid the production of antibod

127 ve acquired a germ cell-specific function in spermatid development.

128 However, round spermatids did not progress beyond step 6, revealing a n

129 he decrease in centrosomal components during spermatid differentiation (spermiogenesis).

130 nd inactive pools, results in abnormal round spermatid differentiation and impaired fertility.

131 lanogaster, many transcripts needed for late spermatid differentiation are synthesized in pre-meiotic

132 ubiquitin proteasome system are required for spermatid differentiation during spermiogenesis.

133 F4G2 mutant germ cells, several key steps of spermatid differentiation fail, including formation of a

134 (SPDS) inhibitor, was added at the start of spermatid differentiation, the spermatid nuclei remained

135 ribed, including the prior report of Ance in spermatid differentiation, Wolbachia-induced sex-specifi

136 tin condensation, cell cycle progression and spermatid differentiation.

137 g the plasma membrane increase necessary for spermatid differentiation.

138 2 function also causes widespread defects in spermatid differentiation.

139 cells to undergo meiotic division and proper spermatid differentiation.

140 otic spermatocytes and postmeiotic arrest of spermatid differentiation.

141 n that specifically binds to the promoter of spermatid-differentiation gene Sdic and identified it as

142 cgrba-activated signaling cascade in haploid spermatids directs gene expression and the progression o

143 rved in spermatids, the Brdt(BD1/BD1) mutant spermatids do not undergo apoptosis (on either backgroun

144 n of cell adhesion most notably in elongated spermatids due to a loss of actin-bundling capability at

145 changes are accompanied by a loss of haploid spermatids due to impeded meiosis.

146 d from cell fragments that were discarded by spermatids during spermiation.

147 g accompanied with the transit of developing spermatids during spermiogenesis must be segregated from

148 roteins (chromatin remodelers, essential for spermatid elongation and completion of spermatogenesis)

149 nslationally activated during the process of spermatid elongation and maturation.

150 Slx/Slxl1 deficiency leads to delay in spermatid elongation and sperm release.

151 e two postmeiotic transitions: initiation of spermatid elongation and spermatozoa release.

152 entiation, meiotic initiation, initiation of spermatid elongation, and release of spermatozoa.

153 ed extensive germ cell apoptosis and blocked spermatid elongation, resulting in severe oligozoospermi

154 We find that asun spermatocytes and spermatids exhibit drastic reduction of perinuclear dyne

155 pporting Sertoli cells, we show that haploid spermatids express the homolog of the tetrapod LHCGR (Lh

156 convergent acquisition and amplification of spermatid-expressed gene families on the X and Y chromos

157 mosomes that multiple families of sex-linked spermatid-expressed genes are highly amplified in Mus mu

158 stinctive simultaneous amplification seen in spermatid-expressed genes.

159 expansion of ampliconic sequence containing spermatid-expressed genes.

160 These multi-nucleated spermatids fail to undergo normal differentiation, leadi

161 t activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway

162 cell division and the production of haploid spermatids from the tetraploid primary spermatocytes via

163 modulations alter 3'-UTR processing in round spermatids; importantly, the BD1 is essential for these

164 d their abrupt disappearance from developing spermatids in a process requiring the mitochondrial nucl

165 zation in cultured cells and is expressed in spermatids in mouse testes.

166 expressed predominantly in spermatocytes and spermatids in mouse, and are reduced in men with impaire

167 T2 correlated with chromatin condensation of spermatids in murine testes.

168 tively expressed mainly by spermatocytes and spermatids in seminiferous tubules of the testis.

169 rough the steps of meiosis to generate round spermatids in testes of rats treated with an acute dose

170 er plasticity to support the transport of 1) spermatids in the adluminal compartment and 2) preleptot

171 seminiferous epithelium, and lack of mature spermatids in the epididymis.

172 ed in the cytoplasm of steps 14-16 elongated spermatids in the mouse testis.

173 TB) must remain intact during the transit of spermatids in the seminiferous epithelium, which is asso

174 immunodetected bound to the Lhcgrba of free spermatids in the SLL, showing that circulating gonadotr

175 , most abundantly expressed in haploid round spermatids in the testis, and the protein is localized t

176 pressed in pachytene spermatocytes and round spermatids in the testis.

177 f elongating/elongated spermatids (step 8-19 spermatids) in the epithelium.

178 matocytes, as well as in round and elongated spermatids, in normal human testes.

179 ed gene (scotti), are male sterile, and show spermatid individualisation defects, indicating a functi

180 severe disruption of the actin cones of the spermatid individualization complex.

181 disrupts the final stage of spermatogenesis, spermatid individualization, and causes male sterility.

182 The associated process, termed spermatid individualization, is facilitated by the apopt

183 hat dmPTB expression is necessary for proper spermatid individualization, the terminal step necessary

184 the actin cones that mediate D. melanogaster spermatid individualization.

185 implicated in Drosophila male fertility and spermatid individualization.

186 males are sterile due to specific defects in spermatid individualization.

187 , intracytoplasmic sperm injection, or round spermatid injection.

188 intracytoplasmic sperm injection, and round spermatid injection.

189 horing junction restructuring at the Sertoli-spermatid interface induced by adjudin which mimics junc

190 n-rich adherens junction at the Sertoli cell-spermatid interface) to coordinate cellular events acros

191 most notably at the apical ES at the Sertoli-spermatid interface, and expressed stage-specifically du

192 ES at the Sertoli cell-elongating/elongated spermatid interface, which is known as apical ES and pos

193 morphological differentiation of the haploid spermatid into spermatozoa.

194 ) fuse with the plasma membrane to transform spermatids into fertilization-competent spermatozoa.

195 ot disrupt meiosis or the differentiation of spermatids into mature sperm.

196 spermiogenesis, the differentiation of round spermatids into mature spermatozoa.

197 ically to regulate the conversion of sessile spermatids into motile spermatozoa, implicating PI(3,4,5

198 red for postmeiotic differentiation of round spermatids into sperm.

199 compromising the release of mature elongate spermatids into the lumen of the seminiferous tubule.

200 Development from round into elongated spermatids is abrogated in Tdrd6(-/-) mice.

201 ure in the nuclei of spermatocytes and round spermatids is essential for correct splicing and the pro

202 m2 mRNA in pachytene spermatocytes and round spermatids is essential for their timely translation at

203 herin, whereas at later stages, apoptosis of spermatids is observed.

204 TR is unexpected because mRNA translation in spermatids is thought to be regulated primarily by the 3

205 In vitro incubation of flatfish spermatids isolated from the SLL with rLh specifically p

206 ions in transcription and histone removal in spermatids, it is unknown whether other BET family prote

207 fragmentation and disruption of the Sertoli-spermatid junctions.

208 racterized by depletion of spermatocytes and spermatids leading to oligoteratozoospermia or azoosperm

209 , disruption of the blood-testis barrier and spermatid loss.

210 hereby destabilizing apical ES to facilitate spermatid loss.

211 wever, only punctate expression of the round spermatid marker SP-10 in the acrosomal granule of germ

212 dentified a previously uncharacterized gene, spermatid maturation 1 (Spem1), encoding a protein exclu

213 During spermatid maturation into sperm, these genes lose H3K4me

214 dividualization complex that is required for spermatid maturation, thereby impairing spermatogenesis.

215 tin remodeling events that take place during spermatid maturation.

216 lved in germ stem/progenitor cells (CDKN2B), spermatid maturation/spermiogenesis (metalloproteinase a

217 dramatically in spermatogenous cells as the spermatids mature.

218 at the apical ES was shown to correlate with spermatid movement and proper spermatid orientation.

219 is-specific Prosalpha6T becomes prominent in spermatid nuclei and cytoplasm after meiosis, and persis

220 ved that a prominent feature of the aberrant spermatid nuclei is a fragmented chromocenter, a structu

221 the start of spermatid differentiation, the spermatid nuclei remained round, centrin failed to local

222 ead and flagellar formation was disrupted in spermatids of MEIG1-deficient mice.

223 not changed in the few remaining elongating spermatids of Meig1-deficient mice.

224 to the manchette in the remaining elongating spermatids of Pacrg-deficient mice, indicating that PACR

225 s of Piwi-interacting small RNAs abundant in spermatids of the adult mouse testis.

226 In the elongating spermatids of wild-type mice, PACRG colocalizes with alp

227 Chromatoid bodies (CBs) are characteristic spermatid organelles, which were suggested to function i

228 Strikingly, spermatid orientation, alignment and release, as well as

229 correlate with spermatid movement and proper spermatid orientation.

230 NF45 are conserved in two related proteins, spermatid perinuclear RNA-binding protein (SPNR) and zin

231 is in vivo was knockdown by RNAi, defects in spermatid polarity and adhesion, as well as spermatid tr

232 pithelial cycle, participating in conferring spermatid polarity and cell adhesion in the testis.

233 spermatid and phagosome transport, and also spermatid polarity due to a disruption of F-actin organi

234 seemed to normally undergo spermatogenesis (spermatid production by meiosis) and spermiogenesis (spe

235 is then specified in the transcribing round spermatid, recapitulating the organization of the human

236 ly abundant in spermatocytes and early round spermatids, regardless of the type of the genomic sequen

237 etached from the nucleus in asun postmeiotic spermatids, resulting in abnormalities later in spermato

238 mmunoprecipitation followed by sequencing in spermatids revealed enrichment of BRD4 and acetylated hi

239 distal and proximal centrioles), but insect spermatids seem to contain only one centriole, which fun

240 aux mutant spermatids show a deficit in formation of the plasma mem

241 matogenesis, SD induces dysfunction of SD(+) spermatids so that SD/SD(+) males sire almost exclusivel

242 Spermatid specific thioredoxin-3 (SPTRX3 or TXNDC8) is a

243 e approach, a mutation was identified in the spermatid-specific "capping protein (actin filament) mus

244 o avoid the production of antibodies against spermatid-specific antigens, many of which express trans

245 Our previous studies using the round spermatid-specific mouse SP-10 gene, which codes for an

246 ption in spermatocytes of an otherwise round spermatid-specific promoter.

247 male and female fertility, promotes haploid spermatid-specific transcription but has distinct roles

248 down-regulation of approximately 300 mostly spermatid-specific transcripts in testis, including near

249 a), pachytene spermatocytes (Spcy) and round spermatids (Sptd) were included.

250 tly expressed from the spermatocyte to round spermatid stage, coinciding with the widespread expressi

251 sis, ranging from the pachytene to the round spermatid stage.

252 of JQ1 evident at the spermatocyte and round spermatid stages cause a complete and reversible contrac

253 mes at the spermatogonial, spermatocyte, and spermatid stages of spermatogenesis.

254 Bsg KO mice was arrested at the early round spermatid stages.

255 arrest of spermatogenesis at an early round spermatid step.

256 intains the polarity of elongating/elongated spermatids (step 8-19 spermatids) in the epithelium.

257 Spermatids subsequently give rise to spermatozoa in the

258 profile, elevated expression in post-meiotic spermatids, suggested proteins that could be involved in

259 uration of spermatogonia, spermatocytes, and spermatids suggests the existence of precise programs of

260 lar remodeling process that trims and shapes spermatid tails.

261 Bam downregulation in spermatocytes affected spermatid terminal differentiation and resulted in incre

262 ownregulation of which is crucial for proper spermatid terminal differentiation.

263 a unique structure at the base of elongating spermatid that directs formation of the flagella.

264 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa,

265 9 elongating spermatids, and those elongated spermatids that were formed lacked the distinctive foci

266 Most animals have two centrioles in spermatids (the distal and proximal centrioles), but ins

267 re fragmented chromocenters were observed in spermatids, the Brdt(BD1/BD1) mutant spermatids do not u

268 In Drosophila spermatids, the giant mitochondria provide structural pl

269 bditis elegans as they activate from a round spermatid to a mature, crawling spermatozoon.

270 RTH-null mice (azoospermic due to failure of spermatids to elongate).

271 ption, even though it fails to support round spermatids to enter spermiogenesis.

272 not required in individual spermatocytes or spermatids to modify sperm.

273 atogenesis and a mutation that allowed these spermatids to self-activate.

274 teleosts, the differentiation of postmeiotic spermatids to spermatozoa (spermiogenesis) is thought to

275 ed piRNAs, lack complementary targets in the spermatid transcriptome.

276 ition of actin branching caused a failure of spermatid transit plus a loss of proper orientation in t

277 spermatid polarity and adhesion, as well as spermatid transport were noted mediated via changes in F

278 Spermatids undergo de novo formation of basal bodies in

279 During spermiogenesis, spermatids undergo dramatic morphological changes includ

280 step in spermatogenesis, when the spherical spermatid undergoes wholesale reorganization to produce

281 It was also noted that the round spermatids underwent eventual degeneration with the form

282 In Lis-1 spermatids, we observed loss of attachments between the

283 ermiogenesis because no elongating/elongated spermatids were detected in any of the tubules examined.

284 atocytes, pachytene spermatocytes, and round spermatids were purified from enzymatically dispersed te

285 y in the absence of Boule, and haploid round spermatids were readily detected.

286 udding, but a small amount remains in budded spermatids where it localizes to MOs as a discrete dot.

287 In round spermatids where the SP-10 gene is expressed, this tethe

288 dine made in the jacket cells moves into the spermatids, where it is involved in the unmasking of sto

289 miogenesis to the centrioles of post-meiotic spermatids, where it reached its greatest concentration

290 on cycles produce seven somatic cells and 32 spermatids, where size and position define identity.

291 ncentrated near the lumen side of elongating spermatids, where structural components of sperm are for

292 ver, TDP-43 remains at the promoter in round spermatids, which express acrv1 mRNA.

293 cific (H1fnt) protein in Brdt(BD1/BD1) round spermatids, which may be linked to the previously report

294 escued via blocking RNF8-MIWI interaction in spermatids with an RNF8-N peptide.

295 ppropriately during meiosis II, resulting in spermatids with disengaged centrioles.

296 s of the meiotic divisions and form aberrant spermatids with large nuclei.

297 stablished during meiosis is maintained into spermatids with the silent compartment postmeiotic sex c

298 Treatment of spermatids with the V-ATPase inhibitor bafilomycin block

299 gment, whereas infertile wpk males developed spermatids with very short flagella that did not extend

300 pachytene spermatocytes and early elongating spermatids without affecting H3K9me3 levels.