ライフサイエンスコーパス: spermatogonial

1 identify neuregulin as a factor required for spermatogonial amplification and differentiation in cult

2 n of germ cells into the pachytene stage, as spermatogonial and Sertoli cells were unaffected in knoc

3 ay, and we now show that irradiation-induced spermatogonial apoptosis is also p53-dependent.

4 XSxr(b)O males have spermatogonial arrest that can be overcome by the re-add

5 cultures on MSC-1 feeder layers resulted in spermatogonial behavior similar to that seen on feeder l

6 rnal age effect lies in the dysregulation of spermatogonial cell behavior, an effect mediated molecul

7 ntation experiments revealed that 6 week-old spermatogonial cell cultures maintained in the presence

8 Zebrafish spermatogonial cell cultures were established from Tg(pi

9 y PM cells is essential for undifferentiated spermatogonial cell development in vivo.

10 the basic signaling pathways that determine spermatogonial cell fate.

11 ion of some markers of stem cell and primary spermatogonial cell fate.

12 The immortalized spermatogonial cell line may serve as a powerful tool in

13 ecause of accumulating replication errors in spermatogonial cell lines.

14 I3-K/AKT/p70S6K/cyclin D3 pathway to promote spermatogonial cell proliferation.

15 anding of the genetic networks that regulate spermatogonial cell renewal and differentiation, and wil

16 Lar is expressed in GSCs and early spermatogonial cells and localizes to the hub-GSC interf

17 N-ethyl N-nitrosourea to induce mutations in spermatogonial cells at an average specific locus rate o

18 ds from telomerase-immortalized mouse type A spermatogonial cells in the presence of stem cell factor

19 use they confer a selective advantage to the spermatogonial cells in which they arise.

20 ion, overexpression of miR-275 or miR-306 in spermatogonial cells resulted in a delay of the prolifer

21 vity specific for the Gli target sequence in spermatogonial cells.

22 F-like molecules stimulated the formation of spermatogonial chain length differently, we purified com

23 are required for effective formation of long spermatogonial chains.

24 M+/HLA-ABC-/CD49e- fraction was enriched for spermatogonial colonizing activity and did not form tumo

25 elf-renewal of SSCs in heterogeneous Thy1(+) spermatogonial cultures over a 63-day period without aff

26 racyst communication, compromise synchronous spermatogonial death and reduces overall germ cell death

27 The recessive juvenile spermatogonial depletion (jsd) mutation results in a sin

28 its sequence results in the sterile juvenile spermatogonial depletion (jsd) phenotype.

29 We identified the gene carrying the juvenile spermatogonial depletion mutation (jsd), a recessive spe

30 rm culture to give rise to multipotent adult spermatogonial-derived stem cells (MASCs).

31 s spontaneously convert to multipotent adult spermatogonial-derived stem cells (MASCs).

32 By coordinating spermatogonial development and mitotic amplification wit

33 SOHLH proteins affect spermatogonial development by directly regulating Gfra1,

34 duction of GDNF by PM cells is essential for spermatogonial development by generating mice with a cKO

35 oduction of GDNF by PM cells is required for spermatogonial development, but PM cells might produce o

36 h1, thereby preventing meiosis and promoting spermatogonial development.

37 PLZF is also essential in osteoblast and spermatogonial development.

38 anslational repression of genes that promote spermatogonial differentiation and (2) repression of the

39 ggest that DMRT6 represses genes involved in spermatogonial differentiation and activates genes requi

40 d, most Dmrt6 mutant germ cells can complete spermatogonial differentiation and enter meiosis, but th

41 Retinoic acid (RA) is a requisite driver of spermatogonial differentiation and entry into meiosis, y

42 Two premeiotic transitions, spermatogonial differentiation and meiotic initiation, w

43 two distinct, cell-type-specific responses: spermatogonial differentiation and meiotic initiation.

44 the transition in gametogenic programs from spermatogonial differentiation and mitosis to spermatocy

45 ansition in spermatogenesis is the exit from spermatogonial differentiation and mitotic proliferation

46 ult males induced, independently, precocious spermatogonial differentiation and precocious meiotic in

47 A-deficient (VAD) mice; (2) the blockage of spermatogonial differentiation by impaired retinoid sign

48 We explored the mechanisms underlying spermatogonial differentiation by targeting expression o

49 scription, and activate transcription of the spermatogonial differentiation factor Sohlh1, thereby pr

50 tiation, causing inappropriate expression of spermatogonial differentiation factors, including SOHLH1

51 d signaling in germ cells completely blocked spermatogonial differentiation identical to vitamin A-de

52 ter, and Nr4a1 expression is diminished upon spermatogonial differentiation in vitro.

53 ate that the action of retinoid signaling on spermatogonial differentiation in vivo is direct through

54 s of Sohlh1 causes infertility by disrupting spermatogonial differentiation into spermatocytes.

55 expression of Hes5, with a reduction of the spermatogonial differentiation marker, Neurog3 expressio

56 whether male germ cells undergo mitosis and spermatogonial differentiation or meiosis.

57 d, the competencies of germ cells to undergo spermatogonial differentiation or meiotic initiation in

58 phase; and (3) retinoid signaling regulated spermatogonial differentiation through controlling expre

59 6J strain, a null mutation in Dmrt6 disrupts spermatogonial differentiation, causing inappropriate ex

60 eas exposure to retinoic acid, an inducer of spermatogonial differentiation, downregulates miR-221/22

61 transitions occurring in physical proximity: spermatogonial differentiation, meiotic initiation, init

62 ls in juvenile mice results in a blockage of spermatogonial differentiation, similar to that seen in

63 turbing spermatogenesis in vivo, focusing on spermatogonial differentiation, which begins a series of

64 Retinoic acid (RA) signaling is crucial for spermatogonial differentiation, which is a key step for

65 maintenance, and induce genes important for spermatogonial differentiation.

66 scriptional regulators that are essential in spermatogonial differentiation.

67 H transcription factor that is essential for spermatogonial differentiation.

68 Little is known about factors necessary for spermatogonial differentiation.

69 oop-helix transcription factor implicated in spermatogonial differentiation.

70 promotes (but is not strictly required for) spermatogonial differentiation.

71 IT, a receptor tyrosine kinase essential for spermatogonial differentiation.

72 ge response pathway in the earliest steps of spermatogonial differentiation.

73 ormation but rather are required for primary spermatogonial divisions in adult males.

74 germ cells lose pluripotency and commit to a spermatogonial fate.

75 In agreement with Rice's hypothesis, spermatogonial genes are over-represented on the X chrom

76 Spermatogonial germ cells are still present but are unab

77 Most vertebrate spermatogonial GSCs appear to adopt an intermediate stra

78 test this hypothesis critically, we examined spermatogonial kinetics and numbers in rats in which the

79 echanism of protection involved an arrest of spermatogonial kinetics.

80 Spermatogenesis was blocked on the spermatogonial level by SRL treatment.

81 tubules exhibiting altered expression of the spermatogonial markers MAGEA4, FGFR3, and phospho-AKT, w

82 s dazl, dnd, nanos3, vasa and piwil1 and the spermatogonial markers plzf and sox17 for at least six w

83 atogenesis can be divided into three stages: spermatogonial mitosis, meiosis of spermatocytes, and sp

84 germ cells inhibits the transition from the spermatogonial mitotic amplification program to spermato

85 esults support proposed selfish selection of spermatogonial mutations affecting growth factor recepto

86 ubset of induced cells properly colonize the spermatogonial niche.

87 Thus, changes in spermatogonial numbers or suppression of their prolifera

88 s a critical rheostat for maintenance of the spermatogonial pool and propose a model whereby negative

89 ess that is supported by an undifferentiated spermatogonial population and transition to a differenti

90 HLH2 proteins are co-expressed in the entire spermatogonial population except in the GFRA1(+) spermat

91 ing approximately 2% of the undifferentiated spermatogonial population in adulthood.

92 s, which is supported by an undifferentiated spermatogonial population.

93 ogeneous epithelial-like or mesenchymal-like spermatogonial populations, with the latter displaying e

94 e examined mutations induced by treatment of spermatogonial (premeiotic) cells with the chemical muta

95 Using spermatogonial progenitor cells (SPCs) as a model system

96 ere, we show that highly proliferative adult spermatogonial progenitor cells (SPCs) can be efficientl

97 germ cells and differentiation of postnatal spermatogonial progenitor cells (SPCs).

98 auses spermatogonia to precociously exit the spermatogonial program and enter meiosis.

99 the introduction of Eif2s3y restores normal spermatogonial proliferation and progression through mei

100 bution is limited to only two genes, Sry and spermatogonial proliferation factor Eif2s3y.

101 s, the testis determinant factor Sry and the spermatogonial proliferation factor Eif2s3y.

102 ize is reduced in these mice due to aberrant spermatogonial proliferation.

103 a Y chromosomal factor essential for normal spermatogonial proliferation.

104 rocess akin to tumorigenesis, termed selfish spermatogonial selection.

105 roplasia), this process is known as "selfish spermatogonial selection." This mechanism favors propaga

106 Spermatogonial self-renewal and differentiation are esse

107 on of genes previously identified as SSC and spermatogonial-specific markers (e.g., zinc-finger and B

108 he system we measured repair outcomes at the spermatogonial, spermatocyte, and spermatid stages of sp

109 Y719F mutation blocks spermatogenesis at the spermatogonial stages and in contrast the KitY567F mutat

110 Spermatogonial stem and progenitor cells (SSCs) generate

111 We evaluated spermatogonial stem cell (SSC) activity in the developin

112 importance of individual miRs in controlling spermatogonial stem cell (SSC) homeostasis has not been

113 and pale (Ap) spermatogonia that make up the spermatogonial stem cell (SSC) pool.

114 s suggest that exogenous factors crucial for spermatogonial stem cell (SSC) self-renewal are conserve

115 , a Bmp type I receptor inhibitor, prolonged spermatogonial stem cell (SSC) survival in culture and e

116 The spermatogonial stem cell (SSC) that supports spermatogen

117 The mechanisms that guide the continuum of spermatogonial stem cell (SSC) to progenitor spermatogon

118 Spermatogonial stem cell (SSC) transplantation has been

119 In contrast, the recently developed spermatogonial stem cell assay system allows quantitatio

120 The lifelong spermatogonial stem cell divisions unique to male germ c

121 We establish a spermatogonial stem cell index (SSCI) that reliably pred

122 The spermatogonial stem cell initiates and maintains spermat

123 of mouse spermatogonial stem cells, a mouse spermatogonial stem cell line and freshly isolated testi

124 and revealed a novel function in regulating spermatogonial stem cell maintenance.

125 erm cells and the identification of a set of spermatogonial stem cell marker transcripts.

126 proliferation is impaired and expression of spermatogonial stem cell markers c-Ret, Plzf, and Stra8

127 manner, implying failure of maintaining the spermatogonial stem cell niche in somatic cells.

128 urotrophic factor (GDNF), a component of the spermatogonial stem cell niche produced by the somatic S

129 g a classic stem cell system, the Drosophila spermatogonial stem cell niche, we reveal daily rhythms

130 nd blood flow are known to contribute to the spermatogonial stem cell niche.

131 The identity of the spermatogonial stem cell population in the undisturbed t

132 We report here that GDNF promotes spermatogonial stem cell proliferation through activatio

133 view the developments that have been made in spermatogonial stem cell research and potential future u

134 estigation of molecular mechanisms governing spermatogonial stem cell self renewal and hierarchical d

135 ruption of ERM have a loss of maintenance of spermatogonial stem cell self-renewal without a block in

136 toli cells in the testis and is required for spermatogonial stem cell self-renewal.

137 TAF4b may be required for the regulation of spermatogonial stem cell specification and proliferation

138 Despite the central importance of the spermatogonial stem cell to male reproduction, little is

139 Spermatogonial stem cell transplantation began as a theo

140 Restoration of fertility following spermatogonial stem cell transplantation in animals sugg

141 We demonstrate spermatogonial stem cell-associated antigens by using an

142 s their repressive state when present in the spermatogonial stem cell.

143 Human adult spermatogonial stem cells (hSSCs) must balance self-rene

144 In this study, spermatogonial stem cells (SSC) that harbor paternalized

145 selfish mutations: substitutions that grant spermatogonial stem cells (SSCs) a selective advantage i

146 Spermatogonial stem cells (SSCs) are a subpopulation of

147 Spermatogonial stem cells (SSCs) are at the foundation o

148 Male germline or spermatogonial stem cells (SSCs) are conserved across ma

149 Spermatogonial stem cells (SSCs) are responsible for mai

150 Spermatogonial stem cells (SSCs) are the basis of sperma

151 Spermatogonial stem cells (SSCs) are the foundation for

152 he untimely and excessive differentiation of spermatogonial stem cells (SSCs) by disturbing the expre

153 Spermatogonial stem cells (SSCs) capable of self-renewal

154 ction of functional sperm from self-renewing spermatogonial stem cells (SSCs) in cell culture conditi

155 erm line stem cells (GSCs) in Drosophila, or spermatogonial stem cells (SSCs) in mammals.

156 Self-renewal and differentiation by spermatogonial stem cells (SSCs) is the foundation for c

157 Self-renewal of spermatogonial stem cells (SSCs) is the foundation for m

158 Spermatogonial stem cells (SSCs) maintain spermatogenesi

159 Spermatogonial stem cells (SSCs) maintain spermatogenesi

160 Postnatal spermatogonial stem cells (SSCs) progress through prolif

161 Self-renewal and differentiation of spermatogonial stem cells (SSCs) provide the foundation

162 Spermatogonial stem cells (SSCs) self-renew and produce

163 permatogenesis is initiated and sustained by spermatogonial stem cells (SSCs) through self-renewal an

164 ent results have demonstrated the ability of spermatogonial stem cells (SSCs) to de-differentiate int

165 In spermatogonial stem cells (SSCs), Myc controls SSC fate

166 We observed a paucity of mutant spermatogonial stem cells (SSCs), which appears independ

167 eating transchromosomic mice by manipulating spermatogonial stem cells (SSCs), which exhibited superi

168 n the GFRA1(+) spermatogonia, which includes spermatogonial stem cells (SSCs).

169 gametes originate from a small population of spermatogonial stem cells (SSCs).

170 level is essential for homeostasis in murine spermatogonial stem cells (SSCs).

171 population size and differentiation fate of spermatogonial stem cells (SSCs).

172 Thus, to proliferate, spermatogonial stem cells activate mechanisms that are s

173 n provides insight into the amplification of spermatogonial stem cells and the origin of primordial f

174 are prospermatogonia), increases steadily as spermatogonial stem cells appear, plateaus as the first

175 This results, in part, from the fact that spermatogonial stem cells are an extremely rare cell pop

176 e generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an

177 Spermatogonial stem cells are required for the initiatio

178 Although spermatogonial stem cells are unipotent, these cells are

179 tal period in the non-dividing precursors of spermatogonial stem cells at a stage where retrotranspos

180 required for the survival of mouse PGCs and spermatogonial stem cells by suppressing apoptosis.

181 Spermatogenesis is the process by which spermatogonial stem cells divide and differentiate to pr

182 Spermatogonial stem cells divide throughout life, mainta

183 Thus, molecular markers specific for spermatogonial stem cells establish a reliable and rapid

184 Recently, transplantation of mouse donor spermatogonial stem cells from a fertile testis to an in

185 Thus, transplantation of spermatogonial stem cells from an infertile donor to a p

186 we examine the feasibility of transplanting spermatogonial stem cells from other species to the mous

187 highlights the in-vitro propagation of human spermatogonial stem cells from testicular biopsies for f

188 First, spermatogonial stem cells from the adult cryptorchid tes

189 consistent with germline selection: mutated spermatogonial stem cells gained an advantage that allow

190 Whereas in-vitro propagation of spermatogonial stem cells has been established in animal

191 antation experiments revealed a depletion of spermatogonial stem cells in the adult.

192 rmatogenesis involves the differentiation of spermatogonial stem cells into spermatocytes via mitotic

193 of the genome, and telomerase expression in spermatogonial stem cells is responsible for the mainten

194 vitro retroviral-mediated gene delivery into spermatogonial stem cells of both adult and immature mic

195 permatogenesis in mouse testes suggests that spermatogonial stem cells of many species could be trans

196 was not functionally redundant with NSun2 in spermatogonial stem cells or Sertoli cells.

197 Division of spermatogonial stem cells produces daughter cells that e

198 ave now devised culture conditions where rat spermatogonial stem cells renew and proliferate in cultu

199 Maintenance of spermatogonial stem cells requires the transcriptional r

200 Spermatogonial stem cells reside in specific niches with

201 ermore, the presence of surface integrins on spermatogonial stem cells suggests that these cells shar

202 ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of

203 after treatment, suggesting that persistent spermatogonial stem cells were not sensitive to NOVP.

204 Transfection of the spermatogonial stem cells with a plasmid containing the

205 ance and differentiation of gonocytes and/or spermatogonial stem cells would be modulated through thi

206 l systems, we used primary cultures of mouse spermatogonial stem cells, a mouse spermatogonial stem c

207 arch surrounds the regenerative potential of spermatogonial stem cells, a recent article highlights t

208 ovessels may contribute to the regulation of spermatogonial stem cells, as well.

209 In contrast to spermatogonial stem cells, gonocytes can be identified e

210 Given STAT3's function in mouse spermatogonial stem cells, we suggest that this interact

211 al germ cells in the embryo and give rise to spermatogonial stem cells, which establish and maintain

212 nuclear factor-Y is required for maintaining spermatogonial stem cells.

213 is: regulation of meiosis and maintenance of spermatogonial stem cells.

214 pment, hematopoiesis, and differentiation of spermatogonial stem cells.

215 ach for purification and characterization of spermatogonial stem cells.

216 es impaired differentiation of embryonic and spermatogonial stem cells.

217 on, and resulted in a 166-fold enrichment of spermatogonial stem cells.

218 tial for reproduction, little is known about spermatogonial stem cells.

219 e cyst cells break apart and probably become spermatogonial stem cells.

220 sequent extensive apoptosis occurring at the spermatogonial stem-cell level.

221 sexes, with males also exhibiting defective spermatogonial stem-cell renewal.

222 ll-autonomously in somatic cells to maintain spermatogonial stemness.

223 mbrane attachment reverting mesenchymal-like spermatogonial subtype cells back to an epithelial-like

224 ein is required for male germ cells to cease spermatogonial TA divisions and initiate spermatocyte di

225 spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of sub

226 f stem cells with basement membranes and our spermatogonial transplantation assay system to identify

227 Spermatogonial transplantation experiments revealed a de

228 In this study, we used the spermatogonial transplantation functional assay, combine

229 We used the technique of spermatogonial transplantation in two infertile mouse st

230 Although the development of the spermatogonial transplantation technique has provided an

231 demonstrate, by using the recently developed spermatogonial transplantation technique, that male germ