ライフサイエンスコーパス: spermatozoa

1 s (spermatid activation into actively motile spermatozoa).

2 s previously thought to be expressed only on spermatozoa.

3 ecific genes and histone retention in mature spermatozoa.

4 flagellum between testicular and epididymal spermatozoa.

5 e BTB and spermatocyte progression to mature spermatozoa.

6 production in treated versus non-treated WT spermatozoa.

7 A1-GFP fusion protein (57 kDa) in transgenic spermatozoa.

8 ifferentiation of the haploid spermatid into spermatozoa.

9 ne recombination of floxed alleles in ~2% of spermatozoa.

10 the superfluous cytoplasm of defective human spermatozoa.

11 lize ova relies upon the swimming ability of spermatozoa.

12 oxidase is expressed and functions in human spermatozoa.

13 studied in fish, aquatic invertebrates, and spermatozoa.

14 equired for optimal superoxide production by spermatozoa.

15 Seminal plasma is not just a carrier for spermatozoa.

16 MO acidification and formation of functional spermatozoa.

17 necessary for acrosomal exocytosis in mouse spermatozoa.

18 P1CC2 is the sole isoform found in mammalian spermatozoa.

19 ss associated with the development of mature spermatozoa.

20 form spermatids into fertilization-competent spermatozoa.

21 med in vitro fertilization cycle using fresh spermatozoa.

22 by MJ33 on fertilization competence of mouse spermatozoa.

23 by antibodies alone are functional in mouse spermatozoa.

24 ing somatic gonad, giving rise to oocytes or spermatozoa.

25 n detected in the head or midpiece of mature spermatozoa.

26 to induce ion channel currents in the whole spermatozoa.

27 ding the egg is a potent stimulator of human spermatozoa.

28 ly diminished hyperactivation of capacitated spermatozoa.

29 tion of spermatid elongation, and release of spermatozoa.

30 seminiferous epithelium for development into spermatozoa.

31 intracellular alkalinization and activating spermatozoa.

32 a, spermatocytes, spermatids, and eventually spermatozoa.

33 ferentiation of round spermatids into mature spermatozoa.

34 competition is the cooperative behaviour of spermatozoa.

35 in germ cells ranging from spermatogonia to spermatozoa.

36 velope and cytoplasmic droplet of epididymal spermatozoa.

37 rial segment, neck and the midpiece of human spermatozoa.

38 ocalized to the centrioles of spermatids and spermatozoa.

39 eriments using motile Caenorhabditis elegans spermatozoa.

40 eteroduplex DNA at crossover sites in mature spermatozoa.

41 at is essential for the production of normal spermatozoa.

42 on of spermatogenesis, and the production of spermatozoa.

43 o normal motility and fertility of mammalian spermatozoa.

44 r that might help explain its unique role in spermatozoa.

45 e head and the neck region of the developing spermatozoa.

46 enrichment of the atypical centrioles in the spermatozoa.

47 e bicarbonate stimulation of sAC activity in spermatozoa.

48 type spermatozoa, do not develop in sAC(-/-) spermatozoa.

49 facilitate evoked Ca(2+) entry into sAC(-/-) spermatozoa.

50 ced male fertility and defective motility of spermatozoa.

51 hinery is not operating normally in sAC(-/-) spermatozoa.

52 hey differentiate through meiosis and become spermatozoa.

53 und impairment in motility and morphology of spermatozoa.

54 for further development and maturation into spermatozoa.

55 e eye and on the inner acrosomal membrane of spermatozoa.

56 ticular morphology and comparable numbers of spermatozoa.

57 rrant miRNA profiles in Dicer and Drosha cKO spermatozoa.

58 ility and ultrastructural disorganization of spermatozoa.

59 oximately 500-fold and is barely detected in spermatozoa.

60 ed expanding clones that gave rise to mature spermatozoa.

61 , however, did not impair the motility of WT spermatozoa.

62 erm-line stem cells, and ending with haploid spermatozoa.

63 erentiating daughter cells for production of spermatozoa.

64 effective but underused method to safeguard spermatozoa.

65 le others differentiate to eventually become spermatozoa.

66 enes potentially involved in the function of spermatozoa.

67 and terminal differentiation into functional spermatozoa.

68 is expressed in ciliated sensory neurons and spermatozoa.

69 Men with >15% of SPTRX3-positive spermatozoa, a cutoff value established by ROC analysis,

70 testis lacks elongated spermatids and mature spermatozoa, a phenotype similar to that of alpha-mannos

71 In spermatozoa, a small proportion of chromatin, which rang

72 Spermatozoa agglutinated head-to-head show contact-induc

73 tilization (IVF) that failed with epididymal spermatozoa alone.

74 spermatids that cannot activate to crawling spermatozoa, although spermatids from mutant males activ

75 hin the periacrosomal region of mature mouse spermatozoa and are thus well positioned to regulate the

76 diminished in crude preparations of sAC(-/-) spermatozoa and are undetectable after sperm purificatio

77 0 nt was extracted from sonicated (SS) mouse spermatozoa and detergent demembranated sucrose gradient

78 substantial remodeling of the glycocalyx of spermatozoa and epididymal epithelial cells by endogenou

79 nic acid residues was detected in epididymal spermatozoa and epithelial cells using combined laser mi

80 Many microorganisms, including spermatozoa and forms of bacteria, oscillate or twist a

81 Here we show that in intact spermatozoa and in a heterologous expression system, the

82 that NOX5 is a major source of ROS in human spermatozoa and indicate a role for NOX5-dependent ROS g

83 evel of Atp6v0a2 is required for the fertile spermatozoa and its decreased level in spermatozoa could

84 required for the hyperactivated motility of spermatozoa and male fertility.

85 ivation of Spata6 in mice leads to acephalic spermatozoa and male sterility.

86 Both spermatozoa and non-motile spermatids bud MSP vesicles,

87 cell viability compared with wild type (WT) spermatozoa and often were detected in large agglutinate

88 We conclude that factors derived from spermatozoa and oocyte spindles may affect the ability o

89 issues linked to immediate removal of excess spermatozoa and preparation of the uterus for implantati

90 number, an increased proportion of abnormal spermatozoa and reduced fertility.

91 ces in metabolism to 3-chlorolactaldehyde in spermatozoa and somatic cells.

92 roxidation, target the mitochondria of human spermatozoa and stimulate mitochondrial superoxide gener

93 her alterations of the sialome of epididymal spermatozoa and surrounding epithelial cells occur durin

94 C plays a critical role in cAMP signaling in spermatozoa and that defective cAMP production prevents

95 en without any apparent cytotoxic effects on spermatozoa and that these structures change along the l

96 fusion pores during the acrosome reaction in spermatozoa and the mobilization of calcium from the acr

97 in the mouse results in a binding defect in spermatozoa and their inability to pass through the uter

98 e, by using a simple approach to patch-clamp spermatozoa and to characterize whole-spermatozoan curre

99 ons, contributes to the prodigious output of spermatozoa and to the elaborate organization of spermat

100 Progesterone induces Ca(2+) influx into spermatozoa and triggers multiple Ca(2+)-dependent physi

101 living single cells (e.g. bacteria, archaea, spermatozoa, and protozoa).

102 nt motile and percent morphologically normal spermatozoa, and testis size.

103 indeed the principal Ca(2+) channel of human spermatozoa, and that it is strongly potentiated by prog

104 the AR in sperm from WT but not alpha7-null spermatozoa, and the induced AR was inhibited by alpha7

105 In the distal epididymis, these spermatozoa appear to lack calcium-dependent association

106 The remaining spermatozoa are abnormal with impaired motility and fert

107 Eutherian spermatozoa are dependent on the environment of the prox

108 shapen and multinucleated spermatids, and no spermatozoa are detected in the epididymis.

109 evelopment in Drosophila ensures that mature spermatozoa are devoid of DNA.

110 pment and increases gradually to a peak when spermatozoa are ejaculated.

111 ored whether steroid hormones to which human spermatozoa are exposed in the male and female genital t

112 Human spermatozoa are quiescent in the male reproductive syste

113 Mature mammalian spermatozoa are quiescent in the male reproductive tract

114 ygous male mice present with massive loss of spermatozoa as a consequence of meiotic failure.

115 nalyses on the testis squash preparation and spermatozoa at a subcellular level indicated that the pr

116 Mammalian spermatozoa become motile at ejaculation, but before the

117 liosides in the plasma membrane of live boar spermatozoa before and after cholesterol reduction.

118 However, although mouse and rat spermatozoa bind to eggs from each rodent, human sperm b

119 Human fertilization begins when spermatozoa bind to the extracellular matrix coating of

120 At fertilization, spermatozoa bind to the zona pellucida (ZP1, ZP2, ZP3) s

121 We show that ABHD2 is highly expressed in spermatozoa, binds progesterone, and acts as a progester

122 ntial for the function of the epididymis and spermatozoa, but ATP release in the epididymis remains u

123 Proteasomes are present in human and boar spermatozoa, but little is known about the interactions

124 ially in membranes of the retina, brain, and spermatozoa, but the functional significance of this lar

125 non-genomic action of progesterone on human spermatozoa by identifying the Ca(2+) channel activated

126 action of Hv1 and CatSper channels in human spermatozoa can induce elevation of both intracellular p

127 sence of RNAs in transcriptionally quiescent spermatozoa can only be derived from transcription that

128 zed round spermatids into polarized amoeboid spermatozoa capable of both motility and fertilization.

129 matogenesis to occur, and that production of spermatozoa capable of fertilization in vivo can take pl

130 In spermatozoa, capacitation, hyperactivation of motility a

131 hat the ultrastructure of the matured sperm (spermatozoa) centrioles is modified dramatically and tha

132 activity and reduced these parameters in WT spermatozoa compared with controls (p </= 0.05).

133 sence leads to impaired peristalsis, reduced spermatozoa concentration in the semen, and, eventually,

134 rtile spermatozoa and its decreased level in spermatozoa could be used to predict male infertility.

135 ng to appreciate that at fertilization human spermatozoa deliver the paternal genome alongside a suit

136 Fertility of spermatozoa depends on maintenance of the mitochondrial

137 ility of the mitochondrial capsule of mature spermatozoa depends on the moonlighting function of Gpx4

138 Mammalian oocytes and spermatozoa derive from fetal cells shared by the sexes.

139 tment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potenti

140 Although human and mouse spermatozoa do not need to be freed from acrosomes, the

141 hich occur late in capacitation of wild-type spermatozoa, do not develop in sAC(-/-) spermatozoa.

142 Third, we evaluated ROS in zebrafish spermatozoa during cryopreservation and its effect on vi

143 2) = 0.89) in untreated populations of human spermatozoa emphasized the pathophysiological significan

144 le germ cells differentiate to become mature spermatozoa, entails dramatic morphological and biochemi

145 Furthermore,Cul4b(Vasa)spermatozoa exhibited defective arrangement of axonemal

146 Functionally, spermatozoa exposed to calcium ionophore, phorbol ester,

147 Mouse spermatozoa express a pH-dependent K(+) current (KSper)

148 Here, we show that the sNHE-null spermatozoa fail to develop the cAMP-dependent protein t

149 l degeneration through apoptosis, failure of spermatozoa flagella formation, elevated blood pressure

150 rotein (mSLLP1) in the equatorial segment of spermatozoa following the acrosome reaction and a role f

151 tic subcellular reorganizations that lead to spermatozoa formation common to a wide range of animals.

152 ered with spermatogenesis proceeding through spermatozoa formation in 13% to 17% of the seminiferous

153 ord, maternal, and paternal blood as well as spermatozoa from 39 families in Crete, Greece, and the U

154 p6v0a2 protein (P<0.05) and mRNA (P<0.05) in spermatozoa from infertile men were significantly lower

155 Furthermore, spermatozoa from L68Q mice exhibited reduced cell viabil

156 Examination of the epididymal fluid and spermatozoa from L68Q mice showed increased levels and d

157 In vitro studies showed that epididymal spermatozoa from L68Q mice were unable to fertilize oocy

158 Spermatozoa from Nhe8(-/-) mice completely lacked acroso

159 Spermatozoa from older knockout mice showed a lower perc

160 swim-up failed to remove all SPTRX3-positive spermatozoa from semen prepared for ART.

161 cent in vitro experiments with extracts from spermatozoa from the nematode Ascaris suum suggest that

162 ce lacking alpha4 are completely sterile and spermatozoa from these mice are unable of fertilizing eg

163 Mammalian spermatozoa gain competence to fertilize an oocyte as th

164 testes, and its absence resulted in reduced spermatozoa generation, lower actin levels in testes, an

165 The percentage of SPTRX3-positive spermatozoa had predictive value for pregnancy after ART

166 Regulation of ion balance in spermatozoa has been shown to be essential for sperm mot

167 Instead, we find that mouse spermatozoa have a cation-nonselective current in the mi

168 te the loss of ATP-gated current, P2rx2(-/-) spermatozoa have normal progressive motility, hyperactiv

169 males is associated with about 50% of mature spermatozoa having reduced motility.

170 r identity of the potassium channel of human spermatozoa (hKSper) is unknown.

171 spe-45 mutant spermatozoa, however, could not complete gamete fusion,

172 conversion of sessile spermatids into motile spermatozoa, implicating PI(3,4,5)P3 signaling in nemato

173 Development of spermatozoa in adult mammalian testis during spermatogen

174 ior to any surgical procedure to try to find spermatozoa in an azoospermic man.

175 emonstrated in capacitated and uncapacitated spermatozoa in low- and high-viscosity media.

176 gth mRNAs from transcriptionally inert human spermatozoa in semen as a non-invasive diagnostic tool f

177 in sperm number and the presence of abnormal spermatozoa in the epididymis.

178 Spermatids subsequently give rise to spermatozoa in the final phase of spermatogenesis, calle

179 na-free mouse oocytes with capacitated mouse spermatozoa in the presence of varying concentrations of

180 o expression and Orco-mediated activation of spermatozoa in the yellow fever mosquito, Aedes aegypti.

181 The migratory abilities of motile human spermatozoa in vivo are essential for natural fertility,

182 the respiratory burst, in pH regulation, in spermatozoa, in apoptosis, and in cancer metastasis.

183 cell viability and motility compared with WT spermatozoa incubated in epididymal fluid from WT mice.

184 Mammalian spermatozoa indeed undergo complex adaptations within th

185 Physiological and pathological processes in spermatozoa involve the production of reactive oxygen sp

186 ocalization of TRP-3/SPE-41 in spe-38 mutant spermatozoa, ionomycin or thapsigargin induced influx of

187 in the site of fertilization in the knockout spermatozoa is associated with a gradual loss of ADAM3 a

188 Reduced forward progression of TAL-deficient spermatozoa is associated with diminished mitochondrial

189 nclude that the ATP-induced current on mouse spermatozoa is mediated by the P2X2 purinergic receptor/

190 gesterone-dependent Ca(2+) influx into human spermatozoa is primarily mediated by cationic channel of

191 ithin the acrosomal vesicle of all mammalian spermatozoa, is a multifunctional protein that mediates

192 ation of stem cells into millions of haploid spermatozoa--is elaborately organized in time and space.

193 schild of phase synchrony of nearby swimming spermatozoa, it has been a working hypothesis that synch

194 idase mimicked the action of acrosome-intact spermatozoa, it is likely that the acrosomal enzymes def

195 pecific CATSPERdelta are infertile and their spermatozoa lack both Ca(2+) current and hyperactivated

196 ggesting that cAMP metabolism is impaired in spermatozoa lacking sNHE.

197 This current is not present in spermatozoa lacking the sperm-specific putative ion chan

198 the mouse ZP but did not inhibit adhesion of spermatozoa lacking zonadhesin.

199 were observed in the majority of the mutant spermatozoa, manifested by low, if any, sperm ATP produc

200 taxa and posit that OR-mediated responses in spermatozoa may represent a general characteristic of in

201 structural abnormalities particularly in the spermatozoa midpiece due to improper oxidation and polym

202 Normal epididymal sperm count, spermatozoa morphology, capacitation, and motility and r

203 on channel, which was recently implicated in spermatozoa motility, was required for optimal superoxid

204 ll autonomously in male germ cells to ensure spermatozoa motility, whereas it functions non-cell-auto

205 e for NOX5-dependent ROS generation in human spermatozoa motility.

206 Mammalian spermatozoa must complete an acrosome reaction prior to

207 Spermatozoa must leave one organism, navigate long dista

208 ed, generation (n = 3 + 3) and also in adult spermatozoa (n = 2 + 2) in both generations.

209 d seminiferous tubule area, testis size, and spermatozoa number and motility without affecting hormon

210 ects meiosis, spermiogenesis and reduces the spermatozoa number in the third generation (F3) male mic

211 tiation of pre-meiotic germ cells, decreased spermatozoa number, an increased proportion of abnormal

212 ype resulted from a combination of decreased spermatozoa number, reduced sperm motility and defective

213 Here, by recording ionic currents from spermatozoa of an infertile CatSper-deficient patient, w

214 land shore region of mmu-miR-15b in both the spermatozoa of F0 mice and the brains of F1 mice.

215 crosomal matrix that retain acrosome reacted spermatozoa on the zona surface prior to penetration.

216 ear the ovum, it is unclear which cues guide spermatozoa on their long journey towards the egg.

217 progesterone is an innate property of human spermatozoa or is acquired as the result of exposure to

218 ine )) were higher in Prdx6 (-/-) than in WT spermatozoa (p </= 0.05).

219 tubules of the testes, producing millions of spermatozoa per day in an adult male in rodents and huma

220 The way that spermatozoa phenotypes evolve is poorly understood, beca

221 Many of the spermatozoa possess an angulated tail with a cytoplasmic

222 male gametes and histone retention in mature spermatozoa, potentially priming certain regions of the

223 radients, alkalization depolarizes Slo3(-/-) spermatozoa, presumably from CatSper activation, in cont

224 sed germ cell apoptosis, effectively halting spermatozoa production.

225 Here, analyses of sAC(-/-) spermatozoa provide additional insights into the functio

226 ve been developed to determine properties of spermatozoa quality but few have been adopted into routi

227 ull sperm as compared to wild-type, and null spermatozoa rapidly lose progressive motility when incub

228 Zan(-/-) males were fertile, and their spermatozoa readily fertilized mouse eggs in vitro.

229 PII) binding and histone retention in mature spermatozoa relative to CTCF-only sites, but little else

230 ions: initiation of spermatid elongation and spermatozoa release.

231 f the single dense-core granule of mammalian spermatozoa relies on the same highly conserved molecule

232 f progesterone upon transcriptionally silent spermatozoa remains unexplained and is believed to be me

233 h haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying post

234 forceful asymmetric motion of hyperactivated spermatozoa requires Ca2+ entry into the sperm tail by a

235 Sea urchin spermatozoa respond to sperm-activating peptides, which

236 ion of epididymal fluid from L68Q mice to WT spermatozoa resulted in a recapitulation of the L68Q phe

237 dymal fluid can be cytotoxic to the maturing spermatozoa resulting in male infertility.

238 imb overexpression, Asl levels are higher in spermatozoa, resulting in embryos with reduced viability

239 and Zan(-/-) males are fertile because their spermatozoa retain adhesion capability that is not speci

240 ype displays variable expressivity such that spermatozoa show a broad range of defects.

241 TAL(-/-) spermatozoa show loss of Deltapsi(m) and mitochondrial m

242 apitulation of the L68Q phenotype in that WT spermatozoa showed reduced cell viability and motility c

243 In mature spermatozoa, SPE-38 is localized to the pseudopod and TR

244 differentiation of postmeiotic spermatids to spermatozoa (spermiogenesis) is thought to be indirectly

245 Human spermatozoa stimulated with progesterone (a product of t

246 hibitors, is expressed in the epididymis and spermatozoa, suggesting specialized roles in reproductio

247 d blastocyst rates were lower in Prdx6 (-/-) spermatozoa than in C57BL/6J wild-type (WT) controls (p

248 centage points' fewer morphologically normal spermatozoa than men with a sleep score of 11-20.

249 olemma were lower in Prdx6 (-/-) capacitated spermatozoa than WT capacitated controls and lower in WT

250 tion-nonselective current in the midpiece of spermatozoa that is activated by external ATP, consisten

251 KSper, a pH-dependent K(+) current in mouse spermatozoa that is critical for fertility, is activated

252 The molecules on mammalian spermatozoa that mediate recognition and binding to the

253 In human spermatozoa, the flagellar voltage-gated proton channel

254 Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane

255 cent protein (GFP) were incubated with mouse spermatozoa, these sperm were highly successful in produ

256 nmotile spermatids are remodeled into motile spermatozoa through a process known as spermiogenesis.

257 eggs also emit chemotactic agents that guide spermatozoa through the female reproductive tract to the

258 possibility that AgOrs mediate responses of spermatozoa to endogenous signaling molecules in A. gamb

259 m heterozygous breeding and an impairment of spermatozoa to fertilize oocytes in vitro.

260 iability, motility, and the ability of mouse spermatozoa to fertilize oocytes.

261 Exposure of human spermatozoa to NO donors caused mobilisation of stored C

262 ss of zonadhesin increased adhesion of mouse spermatozoa to pig, cow, and rabbit ZP but not mouse ZP.

263 e-like protein is involved in the binding of spermatozoa to the egg plasma membrane during fertilizat

264 posed domain inhibited adhesion of wild-type spermatozoa to the mouse ZP but did not inhibit adhesion

265 complete inhibition of binding and fusion of spermatozoa to the oocyte occurred at 12.5 muM concentra

266 Specific binding of spermatozoa to the zona pellucida that surrounds mammali

267 in that mediates binding of acrosome-reacted spermatozoa to zona glycoproteins via a stereospecific p

268 It attracts spermatozoa towards the egg and helps them penetrate the

269 than WT capacitated controls and lower in WT spermatozoa treated with the PRDX6 inhibitor.

270 In spe-38 mutant spermatozoa, TRP-3/SPE-41 is trapped within the MOs and

271 many cellular organelles and proteins as the spermatozoa undergo maturation.

272 have IFT components and, like some metazoan spermatozoa, use IFT-independent mechanisms to build axo

273 te (19.2% pregnant with >15% SPTRX3-positive spermatozoa vs. 41.2% pregnant with <5% SPTRX3-positive

274 sialic acid residues bound to the surface of spermatozoa was documented in men with a recent history

275 d N-acetylneuraminic acid release from human spermatozoa was effectively counteracted by a sialidase

276 irmed, while persistence of both proteins in spermatozoa was revealed for the first time.

277 ng the patch-clamp technique to mature human spermatozoa, we found that nanomolar concentrations of p

278 urrents from human epididymal and testicular spermatozoa, we show that CatSper sensitivity to progest

279 Here, by successfully patch clamping human spermatozoa, we show that proton channel Hv1 is their do

280 hey were fed a high selenium diet, and their spermatozoa were defective and morphologically indisting

281 The best result was obtained when spermatozoa were demembranated individually immediately

282 a2 mRNA and protein, and its localization in spermatozoa were determined.

283 Spermatozoa were retrieved in 37% of patients and in 42.

284 Lentivirally transduced mouse spermatozoa were used in in vitro fertilization (IVF) st

285 High SPTRX3 levels (>15% SPTRX3-positive spermatozoa) were found in 51% of male infertility patie

286 , is localized to the flagella of A. gambiae spermatozoa where Orco-specific agonists, antagonists, a

287 ifically promotes their differentiation into spermatozoa, whereas recombinant follicle-stimulating ho

288 function of MSP raised the question, how do spermatozoa, which are devoid of ribosomes, ER and Golgi

289 n causative; however, extracts from nematode spermatozoa, which use Major Sperm Protein rather than a

290 r localization persisted in ejaculated human spermatozoa, while centriolar TSKS diminished in mouse s

291 ion showed a 1:1 ratio of sSMC(+) to sSMC(-) spermatozoa, while evaluation of sperm aneuploidy status

292 romiscuous and bind to human eggs, but human spermatozoa will not bind to mouse eggs.

293 The swimming stability of spermatozoa with a specified planar beat pattern in the

294 enylyl cyclase (sAC) are sterile and produce spermatozoa with deficits in progressive motility and ar

295 generating transgenic animals by transducing spermatozoa with lentiviral vectors in vitro is a powerf

296 t the acrosomal region and that treatment of spermatozoa with NAADP resulted in a loss of the acrosom

297 merary marker chromosome (sSMC; sSMC(+)) and spermatozoa with normal chromosome complement (sSMC(-)),

298 he human chromosomes 15, 18, X and Y between spermatozoa with the small supernumerary marker chromoso

299 ion of our data, obtained using mature human spermatozoa, with those obtained using bisulfite sequenc

300 lobule lumen (SLL), where they develop into spermatozoa without direct contact with the supporting S