ライフサイエンスコーパス: spheroid

1 function of distance from the surface of the spheroid.

2 rug in the outer proliferating region of the spheroid.

3 and human astrocytes derived in 3D cortical spheroids.

4 mes into three-dimensional cell cultures, or spheroids.

5 ls (EC) were aggregated to create mixed cell spheroids.

6 eradicates hypoxia in FaDu, HCT116 and H1299 spheroids.

7 ne and a loss of epithelial phenotype in CRC spheroids.

8 er drug fluorouracil (5-FU) on HCT116 cancer spheroids.

9 and/or toxic effects on multiple microtissue spheroids.

10 ut increases single cell detachment from the spheroids.

11 ipated by drug-induced disaggregation of the spheroids.

12 tary diffuse leukoencephalopathy with axonal spheroids.

13 for which it is difficult to generate tumour spheroids.

14 s then used for online monitoring of cardiac spheroids.

15 use of co-cultures, surface coatings and 3D spheroids.

16 tary diffuse leukoencephalopathy with axonal spheroids.

17 emergence of S- and N-type cells from mixed spheroids.

18 show no ongoing star formation and are dense spheroids.

19 nd grown into three-dimensional cell culture spheroids.

20 arly mouse embryo and two different cellular spheroids.

21 quenching" relates to the emergence of dense spheroids.

22 nanomedicines into tumor cell monolayers and spheroids.

23 bility in 3D multicellular DU-145 tumor cell spheroids.

24 andomly distributed nanosized fullerene-like spheroids.

25 and poly-l-lysine was employed to coat cell spheroids.

26 e permeability and efficacy in multicellular spheroids.

27 t a depth of approximately 100 mum in tumour spheroids.

28 t days, promoted glucose uptake by the liver spheroids.

29 e-dimensional colon cancer cell cultures, or spheroids.

30 n species-dependent PBMC chemotaxis to HNSCC spheroids.

31 ve 3D structures deep inside (>50 mum) tumor spheroids.

32 PGPs) transform a 2D cell sheet into 3D cell spheroids.

33 ative of label-free viability tests in tumor spheroids.

34 ughout large (>500 microm diameter) 3D tumor spheroids.

35 In connexin-decoupled spheroids, 4,4'-diisothiocyano-2,2'-stilbenedisulfonic a

36 Adult-onset leukoencephalopathy with axonal spheroids, a probably underestimated disorder, is associ

37 c device allows for the dynamic treatment of spheroids across a semipermeable membrane.

38 ree and liposomal doxorubicin throughout the spheroid after just 12 hours of treatment.

39 ion elemental imaging of multicellular tumor spheroids and an approach to account for variations in c

40 n dystrophic swellings that resembled axonal spheroids and contained mitochondria and vesicular prote

41 tural level, we observed formation of axonal spheroids and decreased density of axons in the optic ne

42 tem cell marker as being overexpressed in OC spheroids and directly connected to key elements of the

43 reased apoptosis in MDA-MB-231 breast cancer spheroids and dramatically reduced growth of MDA-MB-231

44 nti-PD-1 antibody increased cell death in 3D spheroids and extended survival of MDA-MB-231-bearing mi

45 In vitro cultures of mouse primary tumor spheroids and human cancer cell lines displayed increase

46 tractile forces to break free from the tumor spheroids and invade into the ECM.

47 d penetration of folinic acid to the core of spheroids and metabolism of the drug in the outer prolif

48 Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) is a frequent cause

49 "adult-onset leukoencephalopathy with axonal spheroids and pigmented glia" (ALSP) has been proposed t

50 in whom the pathognomonic features of axonal spheroids and pigmented microglia have been found.

51 1 patient, brain biopsy demonstrated axonal spheroids and pigmented microglia, which are the pathogn

52 n-regulated ALDH1A1 in the maintenance of OC spheroids and propose new ALDH1A1 inhibitors targeting t

53 ge-like TAMs were localized in the center of spheroids and secreted EGF, which upregulated alphaMbeta

54 344SQ in hydrogels formed spheroids and secreted proangiogenic growth factors that

55 tary diffuse leukoencephalopathy with axonal spheroids and suggest that haematopoietic stem cell tran

56 a strong correlation between TAM-associated spheroids and the clinical pathology of ovarian cancer.

57 xtensively throughout both the multicellular spheroids and the tumor mass.

58 rised by the presence of axonal loss, axonal spheroids and variably present pigmented macrophages on

59 pancreatic cancer cells, multicellular tumor spheroids, and cancerous patient tissues.

60 These ECS cells grow as non-attached spheroids, and display enhanced migration and invasion.

61 activity in tumor cell lines, multicellular spheroids, and mice.

62 techniques for assembling molecules, cells, spheroids, and microgels and achieving bottom-up tissue

63 ile and compare 2D cultures with cancer cell spheroids, and microtissue slices from tumors, and norma

64 junctival epithelium and lacrimal gland cell spheroids, and recapitulates the aqueous and mucin layer

65 asma membrane, induced a patent lumen in CRC spheroids, and slowed CRC cell invasion.

66 Rather, IDH1 mitigated mitochondrial ROS in spheroids, and suppressing IDH1 reduced spheroid growth

67 in collectively invading cells and 3D tumor spheroids, and the in vitro invasive capacity of the car

68 tect the extracellular compounds inside live spheroids, and the successful development of our techniq

69 unded the BCCs, promoted formation of cancer spheroids, and then were internalized/degraded through a

70 ifferent cells during the formation of tumor spheroids, and to track the degradation of vasculature o

71 gs provide the first evidence of intact hPSC spheroid architectures and similar fine structures to 2D

72 It has proven challenging to stabilize spheroid architectures for detailed morphological examin

73 Spheroids are ball-shaped stone objects found in African

74 As the spheroids are comparably sizable, it is difficult to mon

75 Spheroids are either fabricated or naturally shaped ston

76 In addition, HCC cells in EpCAM(+) spheroids are more resistant to chemotherapeutic agents

77 The cells in the spheroids are more round and tightly interacting with ea

78 onstrate that EpCAM(+) HCC cells cultured as spheroids are more sensitive to TGF/beta-induced epithel

79 supramolecular organic frameworks (cuboid or spheroid) are constructed hierarchically from CB[8] and

80 First, spheroid arrays of 11 cell-lines were rapidly assessed f

81 The drug is actively released in the spheroid as the lipids bind to cellular lipid bilayers.

82 They also support the use of 3D spheroids as a predictive alternative to in vivo models

83 CDCP1 mediates formation and survival of OCC spheroids, as well as cell migration and chemoresistance

84 study addresses that challenge with a novel spheroid assay, wherein spheroids, formed by magnetic 3D

85 Similarly, cell proliferation, migration, spheroid attachment and outgrowth on collagen, and Akt p

86 detected necrotic regions within these tumor spheroids based on increased intrinsic optical attenuati

87 the application of spheroid microarrays for spheroid-based drug screens was demonstrated by quantify

88 udy, we have developed a novel multicellular spheroid-based hepatic differentiation protocol starting

89 Compare to 400 mum-thick tissue spheroids bioprinted in a liquid delivery medium into co

90 UCYN-A shares many core genes with the 'spheroid bodies' of Epithemia turgida and the endosymbio

91 hout normal cell-type regionalization, these spheroids bore a resemblance to mammalian tissue organoi

92 lling tumour burden and resistance in tumour spheroids, but not in monolayer culture.

93 mors and corresponding serial xenografts and spheroids by high-coverage whole-genome sequencing, clus

94 Multicellular spheroids can be achieved within 24 h and further booste

95 These subdomain-specific forebrain spheroids can be assembled in vitro to recapitulate the

96 Moreover, already assembled microtissue spheroids can be loaded into the microfluidic structures

97 Cells and microtissue spheroids can be retrieved from the chip by using a para

98 ally, we provide proof-of-principle that PHH spheroids can reflect liver pathologies such as cholesta

99 and ERMS biopsies using a three-dimensional spheroid cell invasion assay.

100 Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using

101 on levels were also detected in CSC-enriched spheroids compared to monolayer cultures of ovarian canc

102 ge-independent growth and self-renewal in 3D-spheroid conditions.

103 ue disorganization, including ectopic neural spheroids containing differentiated neurons normally fou

104 The goal of this study was to validate spheroid contraction as a cytotoxic endpoint using 3T3 f

105 The results of this study validate spheroid contraction within this assay as an easy, biolo

106 ission of lactate (a chemical base) from the spheroid core had an alkalinizing effect on the rim, pro

107 The spheroid core is comprised mainly of astrocytes, while b

108 res outward alignment vectors from the tumor spheroid, corresponding to high invasiveness of LKB1 mut

109 The beating frequency of each cardiac spheroid could be read out in a completely automated fas

110 We isolated and generated two clonal spheroid CSC lines derived from anaplastic thyroid cance

111 in vitro models of the human heart ("cardiac spheroids", CSs) by co-culturing human primary or iPSC-d

112 Spheroid culture altered Chk1 signalling to a more xenog

113 n methods for applicable multicellular tumor spheroid culture models and recent studies related to th

114 g whole proteome analyses, we found that PHH spheroids cultured this way were similar to the liver in

115 Furthermore, three-dimensional spheroid cultures originating from KPC tumors did not en

116 Viability/Cytotoxicity Assay in small tumor spheroid cultures, showing excellent correlation with ex

117 eply into the hypoxic and acidic cores of 3D spheroid cultures, they may enable the safe and efficaci

118 ial screening of stem-cell and multicellular-spheroid cultures.

119 Morphologically, early spheroids derived from PGCCs were indistinguishable from

120 Spheroids derived from single PGCCs grew into a wide spe

121 sed cytoplasmic lactate retention in Colo357 spheroids (diameter 150 mum).

122 genic cell growth and three-dimensional (3D) spheroid disintegration.

123 intravital microscopy, we observe that tumor spheroids display filopodia in vivo, supporting a potent

124 eport that self-assembling multicellular BBB spheroids display reproducible BBB features and function

125 ow changes in the medium conductivity on the spheroid EIS readings has been developed and validated b

126 Current spheroid EIS systems are, however, not suitable for inve

127 counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen f

128 MV3 tumour spheroids embedded in a collagen matrix unravelled the e

129 ior of mixed N-type and S-type multicellular spheroids embedded in three-dimensional collagen gels.

130 testing was developed by bioprinting hepatic spheroids encapsulated in a hydrogel scaffold into a mic

131 Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Forster resonance energy

132 ultured as monolayer and multicellular tumor spheroids for recapitulating in vivo conditions.

133 poor survival, a better understanding of how spheroids form is critical to improving patient outcome,

134 cribed here for the first time, disrupted OC spheroid formation and cell viability (P<0.001).

135 by limited dilution tumourigenicity assays, spheroid formation and flow cytometry.

136 ody neutralization of ICAM-1 in TAMs blunted spheroid formation and ovarian cancer progression in mou

137 indings uncover a mechanism for TAM-mediated spheroid formation and provide a potential target for th

138 PAR1 expression increased spheroid formation and the level of stemness markers and

139 urther, we have determined that TAMs promote spheroid formation and tumor growth at early stages of t

140 Multicellular aggregation and spheroid formation are strongly impaired under acidifica

141 g HCC stemness, is required for EpCAM(+) HCC spheroid formation as well as the maintenance of the aci

142 nd in vivo using a colony formation assay, a spheroid formation assay and a xenograft tumor model.

143 n levels of MEK/ERK, cell proliferation, and spheroid formation compared to parental mock-transfected

144 icroscopy-based quantification of tumor-cell spheroid formation in the absence of cell-substrate adhe

145 ovarian cancer cell invasion, migration and spheroid formation in vitro.

146 altered intracellular signaling and reduced spheroid formation potential.

147 Various spheroid formation techniques have been widely developed

148 cell enrichment, air-lifting culture, and 3D spheroid formation techniques.

149 Loss of RelB significantly inhibited spheroid formation, ALDH expression and activity, chemor

150 Knockdown of NRP-1 inhibits ECS cell spheroid formation, invasion and migration, and attenuat

151 to enhance epidermal squamous cell carcinoma spheroid formation, invasion, and migration.

152 eatic cancer cells reduced proliferation and spheroid formation.

153 uce growth inhibition, apoptosis and prevent spheroid formation.

154 hip is capable of high-throughput GBM cancer spheroids formation, multiple-simultaneous drug administ

155 ica bioreplication (SBR) process employed on spheroids formed from human pluripotent stem cells (hPSC

156 allenge with a novel spheroid assay, wherein spheroids, formed by magnetic 3D bioprinting, contract i

157 ged SOX2 half-life allows improvement of the spheroid-forming capability of the adipose-derived stem

158 D accumulation was associated with increased spheroid-forming capacity during reoxygenation in vitro

159 lts, with the highest number of APP-positive spheroids found prior to puberty.

160 Here we generate three-dimensional spheroids from human pluripotent stem cells that resembl

161 from human throwing) that 81% of a sample of spheroids from the late Acheulean (Bed 3) at the Cave of

162 In addition, the spheroids generated from different human tumor cells exh

163 3s or (14)-3s combined with human PBMC in 3D spheroids generated from target cell lines to mimic the

164 elative increase in inflammatory markers and spheroid-generating tumor-initiating cells (TIC).

165 hough those efforts improved many aspects of spheroid generation, the procedures became complex and a

166 OC spheroids vs monolayers and in successive spheroid generations, suggesting that 3D aggregates are

167 e growth and oscillations of a hollow tissue spheroid growing freely or when confined.

168 S in spheroids, and suppressing IDH1 reduced spheroid growth through a mechanism requiring mitochondr

169 nd ovarian cancer cells directly altered CSC spheroid growth, and clonal variants with high ST6Gal-I

170 ed anchorage-dependent and three-dimensional spheroid growth, survival, and migration of human glioma

171 These spheroids have been employed to investigate the dose-lim

172 toarchitecture using human cerebral cortical spheroids (hCSs) derived from pluripotent stem cells.

173 cortex-like structure, named human cortical spheroids (hCSs), from pluripotent stem cells.

174 diffuse leukoencephalopathy with neuroaxonal spheroids (HDLS) is a hereditary, adult onset leukodystr

175 tary diffuse leukoencephalopathy with axonal spheroids (HDLS), which is caused by macrophage colony-s

176 itary diffuse leukencephalopathy with axonal spheroids" (HDLS) and "pigmentary orthochromatic leukody

177 ely invading breast and head and neck cancer spheroids, here we identify hypoxia, a hallmark of solid

178 s a mechanism of treatment response in tumor spheroid/immunocyte co-cultures.

179 estricted three-dimensional invasion in both spheroid implantation and Transwell paradigms.

180 mediately as cells rearrange and compact the spheroid in relation to viability and cytoskeletal organ

181 ng carcinoma (NSCLC) cell lines, embedded as spheroids in a collagen gel.

182 cent Alexa546 was shown to insert into tumor spheroids in a sequence-specific manner.

183 ulation was able to generate epithelial-only spheroids in defined 3D cultures.

184 ma cells embedded in an agarose gel and cell spheroids in Matrigel.

185 Microtissue spheroids in microfluidic devices are increasingly used

186 e aggregate the cocoa particles into prolate spheroids in micrometers.

187 ver, not suitable for investigating multiple spheroids in parallel over extended time in an automated

188 the formation of multicellular aggregates or spheroids in the peritoneal cavity, which seed abdominal

189 d reduced the tumor-initiating capability of spheroids in tumor xenograft models.

190 successfully inhibited the growth of cancer spheroids in vitro in a panel of primary cell lines in f

191 efficacy toward the HeLa cell-derived tumor spheroids in vitro.

192 tool was validated in cancer cells grown in spheroids, in mouse tumor models in vivo, and in excised

193 otypes are distinct from effects in epiblast spheroids, indicating that they are tissue specific.

194 uced neuroblastoma cell invasion of 3D tumor spheroids into an extracellular matrix.

195 cell migration and three dimensional radial spheroid invasion in collagen.

196 sic and directional migration and halted U87 spheroid invasion in ex vivo brain slices.

197 sphorylation of p130Cas, FAK, PXN and radial spheroid invasion in stem cell lines isolated from human

198 detect small concentration changes by single spheroids is the key to access their metabolism.

199 Isotope tracing revealed that in spheroids, isocitrate/citrate produced reductively in th

200 ng at the margin and the outer layers of the spheroid itself.

201 ral epithelium cultured as three-dimensional spheroids known as organoids.

202 ved epithelium cultured as three-dimensional spheroids known as organoids.

203 hin each part of the morphology of the tumor spheroids (layers of proliferating, quiescent, and necro

204 uman pancreatic islet microtissues and liver spheroids maintaining functional responses up to 15 days

205 still involve laborious pipetting steps for spheroid manipulation such as collection, distribution a

206 ntly reduces the numerous pipetting steps of spheroid manipulation to a single pipetting; therefore,

207 ure of an inner mass were key parameters for spheroid maturation, we sorted spheroids using an automa

208 We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioas

209 Multicellular tumor spheroids (MCTS) are valuable in vitro tumor models freq

210 ysiologic information of multicellular tumor spheroids (MCTS) growing from approximately 250 to 600 m

211 lture models, especially multicellular tumor spheroids (MCTS), has increased significantly in recent

212 Last, the application of spheroid microarrays for spheroid-based drug screens was

213 ntially increased delivery across a cellular spheroid model of the blood-brain barrier.

214 as mimicked after repeated-dosing in the PHH spheroid model, not possible to detect using previous in

215 Here, using a three-dimensional spheroid model, we show that cytoskeletal filaments do n

216 val under hypoxia and in a three-dimensional spheroid model.

217 ayer cell model and a 3D multicellular tumor spheroid model.

218 Three-dimensional (3D) tumor spheroid models have gained increased recognition as imp

219 Multicellular tumor spheroid models serve as an important three-dimensional

220 ions of the methodology for investigating 3D spheroid morphology and marker expression and for in vit

221 nes were rapidly assessed for differences in spheroid morphology.

222 lines and human tumors to gemcitabine in 3D spheroid, mouse xenografts, and patient-derived xenograf

223 For the first time, a tumor spheroid of 500 mum diameter and consisting of 9000 cell

224 Endothelial cells and 3D spheroids of cervical tumor cells were co-cultured in th

225 By analysing YAP function in 3D spheroids of human cells, we identify the Rho GTPase act

226 0 dysfunction was rescued in 3D cell culture spheroids of primary murine kidney cells after exposure

227 ermed as "bubble domains"-laterally confined spheroids of sub-10 nm size with local dipoles self-alig

228 In particular, tumor spheroids of two cell lines, glioblastoma (U-87MG) and c

229 hieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and c

230 rameters from single human HepaRG hepatocyte spheroids online and continuously with electrochemical m

231 enteroids grown either as three-dimensional spheroids or two-dimensional monolayers.

232 ssion altered cell morphology, reduced tumor spheroid outgrowth, and increased sensitivity to anoikis

233 hanges in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.

234 strategies are needed to target microscopic spheroids persisting in the peritoneal fluid after debul

235 formation suggests a steeply dipping prolate-spheroid pressure source beneath the eastern caldera tha

236 ) emerges as a viable alternative to probing spheroid properties.

237 Furthermore, PHH spheroids remained phenotypically stable and retained mo

238 have enabled the generation of organoids or spheroids representing a variety of tissues, including t

239 In ovarian cancer (OC), spheroids serve as a vehicle for cancer cell disseminati

240 Multicellular spheroids serve as an excellent platform to study tissue

241 nd silencing GRM3 in TMD cells altered their spheroid shape closer to that of BMD cells.

242 ly recently have studies using multicellular spheroids shown an important role for the mechanics of t

243 Thus, spheroid size can be used as a simple metric for toxicit

244 r one enhanced mitochondrial ROS and reduced spheroid size, as did deletion of the mitochondrial citr

245 In cancer cell spheroids, SLC4A4 or SLC4A9 disruption by either genetic

246 92 expression and reduced tube formation and spheroid sprouting of endothelial cells in vitro.

247 on, in vitro endothelial tube formation, and spheroid sprouting.

248 sing a human umbilical vein endothelial cell spheroid-sprouting assay, we found CLEC14A to be a regul

249 Mathematical modelling and layered or spheroid stroma-extracellular matrix-tumour cultures wer

250 However, in 3D spheroid structures having size >200 mum, T and O2 gradi

251 The spheroid surface exhibits high expression of tight junct

252 ensional cultures, epiblast-stage hPSCs form spheroids surrounding hollow, amniotic-like cavities.

253 vely characterized an easily scalable 3D PHH spheroid system in chemically-defined, serum-free condit

254 mbined, our results demonstrate that the PHH spheroid system presented here constitutes a versatile a

255 epithelial markers formed a higher number of spheroids than CD13(+) or CD90(+) CSCs.

256 dextran aqueous two phase system to generate spheroids that are both consistent in size and precisely

257 n vivo-like multicellular structures such as spheroids that cannot be obtained in two-dimensional (2D

258 lginate scaffolds can generate organoid-like spheroids that mimic numerous features of glandular epit

259 al development and disease, and for deriving spheroids that resemble other brain regions to assemble

260 focusing on measurable parameters of hindgut spheroids, the intermediate step between definitive endo

261 uced capability to migrate into breast tumor spheroids, the majority of cells remaining at the margin

262 ops of fresh medium to the wells the contain spheroids, they can be simply settled and attached to th

263 n be implanted into live multicellular tumor spheroids to accumulate the extracellular metabolites pr

264 ge analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly

265 Finally, we have utilized the BBB spheroids to screen and identify BBB-penetrant cell-pene

266 r drug irinotecan and its metabolites inside spheroids treated under a series of conditions.

267 OCT was shown to be capable of evaluating 3D spheroid treatment response even when traditional viabil

268 The MDA-MB-231 embedded spheroid tumor model exhibited the most robust response

269 CF7: a) a 3D collagen embedded multicellular spheroid tumor model, which reflects the architecture an

270 imensional (3D) multicellular aggregates (or spheroids) under non-adherent culture conditions.

271 Increased lactate production by spheroids upon suppression of the aerobic metabolism was

272 osphorus and platinum in HCT116 colon cancer spheroids upon treatment with the clinically used antica

273 time oxygen mapping across a melanoma tumour spheroid using one-photon phosphorescence lifetime imagi

274 arameters for spheroid maturation, we sorted spheroids using an automated micropipette aspiration and

275 Evoked lactate dynamics, imaged in Colo357 spheroids using cytoplasmic pH as a read-out, indicated

276 Spheroids varied by several structural parameters: cell

277 and ALDH1A1 expression were increased in OC spheroids vs monolayers and in successive spheroid gener

278 e and growth as anchorage-independent tumour spheroids was accompanied by changes in both glucose and

279 Long-term growth in serial xenografts and spheroids was driven by multiple genomic subclones with

280 Simultaneous EIS monitoring of up to 15 spheroids was performed in parallel over 4 days at a tem

281 The growth of H23 multicellular spheroids was significantly reduced by 3-mercaptopicolin

282 To identify new targetable molecules in OC spheroids, we investigated gene expression profiles and

283 Spheroids were also imaged to assess the spatial distrib

284 HNSCC spheroids were co-cultured in vitro with peripheral bloo

285 Spheroids were dosed with liposomal doxorubicin, free do

286 Following dosing, the spheroids were harvested for quantitative proteomic prof

287 We found that 13% of spheroids were pre-organoids that matured into intestina

288 These spheroids were then dosed with a common combination chem

289 xidation of both nutrients was suppressed in spheroids, whereas reductive formation of citrate from g

290 ulturing of individual cells and microtissue spheroids, which is based on the hanging-drop network co

291 mors sorted for alpha2-6 sialylation grew as spheroids, while cells lacking alpha2-6 sialylation rema

292 ation stimulated glucose uptake by the liver spheroids, while the latter, in the absence of insulin,

293 In contrast, BMD cells formed a spheroid with a smoother and more circular surface when

294 romolar doses towards 3D multicellular tumor spheroids with 2-photon red light.

295 We discovered that populations of spheroids with a diameter greater than 75 mum and an inn

296 Pre-coating was achieved by treating MIN-6 spheroids with calcium chloride, which enabled the adhes

297 Furthermore, treatment of spheroids with S0859, a small-molecule inhibitor of sodi

298 centration, size and shape of fullerene-like spheroids with tailored topological connectivity to grap

299 used to culture robust pancreatic MIN-6 cell spheroids within 24 hours that were shown to exhibit cel

300 ery patch and a microwell array to form cell spheroids without cell loss.