ライフサイエンスコーパス: spike protein

1 y with moesin (membrane-organizing extension spike protein).

2 inant viruses were determined largely by the spike protein.

3 ion is mediated by the E1 subunit of the SFV spike protein.

4 S1) and fusion-inducing (S2) subunits of the spike protein.

5 s differing in a hypervariable region of the spike protein.

6 topes in the context of the full-length TGEV spike protein.

7 s prompted us to examine the cleavage of the spike protein.

8 pH-induced conformational changes in the SFV spike protein.

9 bited membrane fusion induced by the MHV JHM spike protein.

10 ect from infection with CoVs using the novel spike protein.

11 antibodies (MAbs) to the ectodomain of HKU1 spike protein.

12 eceptor-binding domain (RBD) of the MERS-CoV Spike protein.

13 proteins, VP6, and two domains from VP4, the spike protein.

14 erences in ground states of their respective spike proteins.

15 ed sites on acid-treated virions or isolated spike proteins.

16 llular processing of newly synthesized viral spike proteins.

17 d with affinities of the receptors for viral spike proteins.

18 tibodies specific for both bat and human CoV Spike proteins.

19 onvoluted extracted ion chromatograms of the spiked proteins.

20 litis replicon particles expressing MERS-CoV spike protein].

21 brane fusion reaction catalyzed by the virus spike protein, a complex containing E1 and E2 transmembr

22 sly undergoes genetic changes to its surface spike protein, a major target of neutralizing antibodies

23 lls in vitro with the murine hepatitis virus spike protein, a natural ligand for the N-domain of CEAC

24 its hACE2-dependent transduction by SARS-CoV spike protein, a successful application of the hot spot

25 eptor-binding domain (RBD) from the MERS-CoV spike protein and determined its crystal structure.

26 he receptor-binding domain (RBD) of MERS-CoV spike protein and DPP4 was determined by crystallography

27 ned receptor-binding domain (RBD) on a viral spike protein and its host receptor, angiotensin-convert

28 y requires specific interactions between the spike protein and lipid rafts, probably during the virus

29 The important roles of the spike protein and other structural proteins in murine co

30 pread, attributed to determinants within the spike protein and possibly perpetuated by suboptimal CD8

31 eceptor-binding domain (RBD) of the MERS-CoV spike protein and thereby competitively blocks the bindi

32 hepatotropism of MHV-JHM depends not on the spike protein and viral entry but rather on a combinatio

33 membrane in a reaction requiring both viral spike proteins and nucleocapsid.

34 ace biotinylation of newly synthesized virus spike proteins and retrieval of biotinylated virions usi

35 ectin from hosts, incorporated it into their spike protein, and evolved it into viral receptor-bindin

36 tes that consisted of the same protruding or spike protein antigens of the three viruses in two forma

37 These spike proteins are native as judged by soluble CD4 (sCD4

38 ns recognize different receptors and how the spike proteins are regulated to undergo conformational t

39 annose-type glycans (HMTGs) decorating viral spike proteins are targets for virus neutralization.

40 nating disease, and our model identifies the spike protein as a therapeutic target to prevent axonal

41 MHV) uses the N-terminal domain (NTD) of its spike protein as its receptor-binding domain.

42 y (ca. 70%) of Stx phages via conserved tail spike proteins associated with a short-tailed morphology

43 trigger conformational changes in the viral spike protein at 37 degrees C that facilitate virus entr

44 correlated with the continued degradation of spike proteins at all times of virus infection in sterol

45 Studies of cell surface spike proteins at early times of infection showed that t

46 hus, the data suggest that the domain of the spike protein between amino acids 417 and 547 is require

47 Furthermore, MHV spike protein bound to nonraftraft membrane at 4 degrees

48 , inhibit cell infection, and cause envelope spike protein breakdown, including gp120 shedding and, f

49 that deletion of this 197-aa fragment in the spike protein can attenuate a highly virulent PEDV, but

50 Because the phage tail spike protein complex acts as a membrane-penetrating str

51 urine ( approximately 1.5 muM total protein; spiked protein concentrations were 0.067% of the overall

52 gn for emerging CoVs should involve chimeric spike protein containing neutralizing epitopes from mult

53 e receptor-binding S1 subunit of coronavirus spike proteins contains two distinctive domains, the N-t

54 In contrast, at later times of infection, spike protein degradation was markedly reduced and effic

55 a, the receptor-binding S1 subunits of their spike proteins differ in primary, secondary, and tertiar

56 dding was unaffected by the stability of the spike protein dimer but was a function of the host cell.

57 Thus, cleavage of the spike protein does not seem to be essential for entry an

58 novel T cell epitopes were identified, with spike protein dominating total T cell responses.

59 Fusion is mediated by the spike protein E1 subunit, an integral membrane protein t

60 The spike protein exists in two structurally distinct confor

61 myelinating strains of MHV, differing in the spike protein expressed, infect neurons and glial cells

62 y be correlated with the high specificity of spike proteins for such glycans expressed in the intesti

63 was able to detect differential abundance of spiked proteins for expected ratios >/=2, with comparabl

64 m should include a panel of nucleocapsid and spike proteins from phylogenetically distinct CoVs.

65 ing protein B, the capsid protein F, and the spike protein G.

66 st the CD4 binding site (CD4bs) on the HIV-1 spike protein gp120 can show exceptional potency and bre

67 , gp26), and six trimeric copies of the tail-spike protein (gp9).

68 However, MHV spike protein has an inherent ability to associate with

69 Here, we examine the influenza A virus spike protein hemagglutinin (HA), which undergoes a dyna

70 The major spike protein hemagglutinin binds sialic acid residues o

71 tides corresponding to sequences of SARS-CoV spike protein HR1 and HR2 regions and investigated the i

72 the structures and functions of coronavirus spike proteins, illustrating how the two S1 domains reco

73 respiratory syndrome coronavirus (SARS-CoV) spike protein in cDNA-transfected mammalian cells.

74 ese findings identified a novel role for the spike protein in regulating the uncoating and delivery o

75 We also found that the viral spike protein in the plasma membrane of the infected cel

76 like MHV-2, thus establishing a role for the spike protein in viral growth.

77 The structures of the capsid and spike proteins in the chimeric particle remain unchanged

78 an LOQ of 10 ng/mL was achieved for the two spiked proteins in nondepleted human serum.

79 the viral coat protein and facilitates minor spike protein incorporation.

80 C (379-388) and A (521-531) epitopes of the spike protein inserted into the 987P major fimbrial subu

81 Proteolytic cleavage of the VP4 outer capsid spike protein into VP8* and VP5* proteins is required fo

82 ndent on the proteolytic cleavage of the VP4 spike protein into VP8* and VP5* proteins.

83 The coronavirus spike protein is a multifunctional molecular machine tha

84 The SFV spike protein is composed of a dimer of E1 and E2 transm

85 at the receptor-binding domain (RBD) of HKU1 spike protein is located in the C domain, where the spik

86 ] and non-P[6] strains suggests that the VP4 spike protein is most likely one of the main reasons pre

87 To determine whether the spike protein is responsible for the difference, a recom

88 Exposed epitopes of the spike protein may be recognized by neutralizing antibodi

89 ctively inhibit MERS-CoV replication and its spike protein-mediated cell-cell fusion.

90 Thus, this region of the spike protein might be a target for generation of therap

91 inding to the target cell, the transmembrane spike protein might change conformation by association b

92 se plasma samples, ByOnic consistently found spiked proteins missed by the other tools.

93 prefusion to postfusion conformation of the spike protein must be triggered, leading to membrane fus

94 The spike protein N-terminal domains (NTDs) of bovine corona

95 with Z3A5, a monoclonal antibody against the spike protein of bovine coronavirus (BCV), on an indirec

96 In this study, we showed that for the spike protein of HKU1, the purified C domain, downstream

97 The spike protein of MERS-CoV (MERS-S) facilitates viral ent

98 acids, containing the 5B19 epitope from the spike protein of murine hepatitis virus (MHV) and giving

99 against this fragment recognized the native spike protein of SARS CoV in both monomeric and trimeric

100 Consistently, recombinant full-length spike protein of SARS-CoV or its receptor-binding domain

101 rotein is located in the C domain, where the spike proteins of alpha-CoVs and beta-CoVs in groups B a

102 uman lung tissue and cleave and activate the spike proteins of the Middle East respiratory syndrome a

103 Exchanging the spike proteins of the two viruses neither increased repl

104 The envelope glycoprotein, termed the spike protein, of severe acute respiratory syndrome coro

105 erential effects of certain mutations in the spike protein on 80R versus ACE2 binding, including esca

106 red in a murine model a range of recombinant spike protein or inactivated whole-virus vaccine candida

107 his study shows that formulation of SARS-CoV spike protein or inactivated whole-virus vaccines with n

108 osable with that of the tail-less phage PRD1 spike protein P5 and the adenovirus knob, domains that i

109 Modeling the binding energies of MERS-CoV spike protein RBD to DPP4 of human (susceptible) or hams

110 determined the crystal structure of NL63-CoV spike protein receptor-binding domain (RBD) complexed wi

111 these two critical functions of coronavirus spike proteins, receptor recognition and membrane fusion

112 roperties of genetically engineered isogenic spike protein recombinant demyelinating and nondemyelina

113 , and most significantly, identifies a novel spike protein region involved in the virus cholesterol r

114 In keeping with the previous spike protein results, G91A virus showed limited seconda

115 nants which express either the wild-type JHM spike protein (RJHM) or spike containing the N514S mutat

116 The coronaviral surface spike protein S is a type I transmembrane glycoprotein t

117 studies indicate that SARS-coronavirus (CoV) spike protein (S protein) and its truncated fragments ar

118 The coronavirus spike protein (S) forms the distinctive virion surface s

119 Using synthetic biology, we engineered the spike protein (S) from a civet strain, SZ16, into our ep

120 nistration of BHPIV3 expressing the SARS-CoV spike protein (S) induced a high titer of SARS-CoV-neutr

121 The spike protein (S) of SARS coronavirus (SARS-CoV) attache

122 The coronavirus spike protein (S) plays a key role in the early steps of

123 The full-length spike protein (S) was newly synthesized as an endoglycos

124 rus enters cells through the activities of a spike protein (S) which has receptor-binding (S1) and me

125 The spike protein (S), a membrane component of severe acute

126 d animals all had antibody responses against spike protein S1 fragment and T-cell responses against t

127 d SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleoc

128 lix bundle fusion core structure of MERS-CoV spike protein S2 subunit by X-ray crystallography and bi

129 evere acute respiratory syndrome coronavirus spike protein (SARS-CoV S) can be primed by a variety of

130 -free top-down quantitation strategies using spiked proteins, spectral counting, along with normalize

131 A monoclonal antibody (MAb) (Z3A5) against spike protein subunit of bovine coronavirus (BCV) reacte

132 The E1 spike protein subunit of Semliki Forest virus (SFV) trig

133 Our results identify a region of the SFV E2 spike protein subunit that regulates the pH dependence o

134 rred by a single amino acid change in the E1 spike protein subunit, proline 226 to serine, that incre

135 of plus- and minus-sense subgenomic RNAs and spike protein than WT virus.

136 gh neurovirulence is associated with the JHM spike protein, the protein responsible for attachment to

137 n group A, uses the galectin-like NTD in its spike protein to bind its receptor protein, while HCoV-O

138 exposed as part of the reorganization of the spike protein to its fusion-active conformation.

139 been shown to block binding of the SARS-CoV spike protein to its receptor.

140 rom the metastable, nonfusogenic form of the spike protein to the highly stable form involved in fusi

141 E1s cleavage required spike protein transport out of the endoplasmic reticulum

142 eutralizing antibody treatment or a MERS-CoV spike protein vaccine protected the engineered mice agai

143 istally located VP8* domain of the rotavirus spike protein VP4 (ref. 5) mediates such interactions.

144 The spike protein VP4 is a key component of the membrane pen

145 The spike protein VP4 is a principal component in the entry

146 rted the identification of the P type of the spike protein VP4 of four lapine rotavirus strains as be

147 Interaction of the VP8* domain of the spike protein VP4 with sialic acid was thought to be the

148 us cell attachment protein, the outer capsid spike protein VP4, contains the sequence GDE(A) recogniz

149 quence alterations in the VP8* domain of the spike protein VP4.

150 h a series of intersubunit interactions, the spike protein (VP4) adopts a dimeric appearance above th

151 ntry by trypsin cleavage of the outer capsid spike protein, VP4, into a hemagglutinin, VP8*, and a me

152 us for efficient infectivity by cleaving the spike protein, VP4, into VP8* and VP5*.

153 The rotavirus spike protein, VP4, is a major determinant of infectivit

154 Cleavage of the rotavirus spike protein, VP4, is required for rotavirus-induced me

155 Mechanisms by which the viral spike protein, VP4, mediates receptor binding and membra

156 t the VP5 cleavage fragment of the rotavirus spike protein, VP4, undergoes a foldback rearrangement t

157 are functions of the rotavirus outer capsid spike protein, VP4.

158 tryptic cleavage product of the outer capsid spike protein, VP4.

159 Our results suggest that the spike protein VP8* of RVs is involved in the recognition

160 this study, we demonstrated that the surface spike protein VP8* of the major P genotypes of human RVs

161 The distal portion of rotavirus (RV) VP4 spike protein (VP8*) is implicated in binding to cellula

162 (S-II) containing predicted epitopes of the spike protein was expressed in Escherichia coli.

163 peptide, and mutations in this domain of the spike protein were previously shown to shift the pH thre

164 After low pH treatment, G91A spike proteins were shown to bind conformation-specific

165 RCV]) was mediated through the N but not the spike protein, whereas weaker cross-reactivity occurred

166 affinities and block the binding of MERS-CoV Spike protein with its hDPP4 receptor.

167 tion, consistent with the association of the spike protein with lipid rafts in the plasma membrane.

168 stribution for the functional domains of the spike proteins within the structure of wild-type Sindbis