ライフサイエンスコーパス: spin label

1 nsory cortex activity (quantitative arterial spin labeling).

2 uces >90% helicity and is unperturbed by the spin label.

3 n those found with the commonly used protein spin label.

4 while SERCA was reacted with a Cys-specific spin label.

5 tances between conformationally well-defined spin labels.

6 de, MARCKS-ED, to membranes with and without spin labels.

7 ould be individually modified with nitroxide spin labels.

8 s, and attenuations from micelle-integrating spin labels.

9 five natural side chains were replaced with spin labels.

10 e LHCII trimers in which only one monomer is spin-labeled.

11 s on T4 lysozyme introduced by site-directed spin labeling.

12 e thiosulfonate spin label for site-directed spin labeling.

13 erfusion decrement using continuous arterial spin labeling.

14 images and to perfusion images from arterial spin labeling.

15 flow (rCBF) using pseudo-continuous arterial spin labelling.

16 seudo-continuous magnetic resonance-arterial spin labeling 20 +/- 6 hours before and after TMS treatm

17 In contrast, both the proteins and the spin label alone, when in a glycerol-water mixture below

18 This was accomplished by synthesizing a spin labeled alpha-CD (the wheel) that was mechanically

19 port the genetic encoding of a noncanonical, spin-labeled amino acid in Escherichia coli.

20 EPR of unphosphorylated Noxa, with spin-labeled amino acid TOAC incorporated within the BH3

21 ral perturbations by the bulkier diamagnetic spin label analog.

22 EPR studies with a spin-labeled analogue of stearic acid detected a high-af

23 Fluorescence quenching studies with the spin-labeled analogue showed that the binding site detec

24 Here, we use site-directed spin labeling and a novel total internal reflection fluo

25 ealthy volunteers were scanned with arterial spin labeling and a separate 15 with BOLD.

26 Arterial spin labeling and asymmetric spin echo sequences measure

27 We have quantified both site-directed spin labeling and dehydroalanine formation.

28 Here we report a systematic spin labeling and double electron electron resonance (DE

29 the cytoplasmic surface using site-directed spin labeling and double electron-electron resonance spe

30 hyl-based labels, approach for site-directed spin labeling and efficient immobilization procedure tha

31 the substituted domains using thiol-specific spin labeling and electron paramagnetic resonance (EPR)

32 ious compositions, and initial site-directed spin labeling and electron paramagnetic resonance (EPR)

33 Here we used site-directed spin labeling and electron paramagnetic resonance (EPR)

34 e assessed using voxel-based pulsed arterial spin labeling and morphometric analyses and tested for a

35 Site-directed spin labeling and pulsed electron-electron double resona

36 Using site-directed spin labeling and pulsed electron-electron double resona

37 Arterial spin labeling and seed-based resting state functional co

38 Participants underwent two arterial spin labeling and two blood oxygen level-dependent scans

39 Using site-directed spin labelling and electron paramagnetic resonance spect

40 eine mutants in a soluble CNBD fragment were spin-labeled, and interspin label distance distributions

41 s the successful detection of antigen with a spin labeled antibody fragment by continuous-wave electr

42 -functionalized gold nanoparticles through a spin label are presented.

43 Our simulations show that the depths of spin labels are approximately 6-17 A deeper than the unl

44 Nitroxide spin labels are used for double electron-electron resona

45 netic resonance imaging methods for Arterial Spin Labeling (ASL) and Blood Oxygenation Level Dependen

46 Purpose To compare arterial spin labeling (ASL) data between low- and high-grade bra

47 Arterial spin labeling (ASL) is a magnetic resonance (MR) imaging

48 collateral vessels identified using arterial spin labeling (ASL) magnetic resonance imaging, a techni

49 the emergence and potential role of arterial spin labeling (ASL) MRI, which measures cerebral blood f

50 Here, we investigated arterial spin labeling (ASL) perfusion CMR as a novel approach to

51 ate pattern recognition analysis of arterial spin labeling (ASL) perfusion maps can be used for class

52 diffusion tensor imaging (DTI) and arterial spin labeling (ASL) to discriminate patients with early

53 ical magnetic resonance scans using arterial spin labeling (ASL) were performed to study the haemodyn

54 plementary neuroimaging techniques: arterial spin labeling (ASL), blood oxygen level-dependent (BOLD)

55 nges, as assessed using whole-brain arterial spin labeling (ASL), during tDCS applied to the left DLP

56 nsor imaging (DTI) acquisitions and arterial spin labeling (ASL).

57 Using arterial spin labelled (ASL) magnetic resonance imaging, this is

58 CBF) alterations in IGE detected by arterial spin labelling (ASL) perfusion magnetic resonance imagin

59 EPR spectra of tethered Abeta40 with spin labels at 18 different positions show that Abeta40

60 EPR) approaches: the rotational diffusion of spin labels at 55 residues with continuous-wave EPR, and

61 copy, we observed that the distances between spin labels at positions 311 and 328 in the fibril core

62 RNA substrates spin-labelled at either the 3' or 5' terminus result in

63 ron paramagnetic resonance spectroscopy with spin-labeled ATP analogs to probe the structure of the A

64 and diffusion dynamics in the vicinity of a spin label attached to a cysteine in the Tyr71 --> Cys G

65 an alternative sequence in which a nitroxide spin label attached to cysteine has been introduced at i

66 nonaribonucleotide pUUCGUAAAA with nitroxide spin labels attached to the 5'-phosphate and to the C8 a

67 by exploring the distance histograms between spin-labels attached to T4 lysozyme.

68 of brain activity using continuous arterial spin labeling based functional magnetic resonance imagin

69 synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points

70 eins, synthesizing the necessary quantity of spin-labeled biomolecules (typically 50 pmol to 100 pmol

71 idual magnetic molecules, nanostructures and spin-labelled biomolecules.

72 tion proceeded efficiently with fluorescent, spin-labeled, biotinylated, or cross-linker-modified gua

73 rotonated fatty acid and phosphatidylcholine spin labels, both of which have a considerably lower aff

74 ramagnetic resonance (EPR) of a bifunctional spin label (BSL) bound stereospecifically to Dictyosteli

75 he actin-binding cleft, using a bifunctional spin label (BSL).

76 tice relaxation rate (T1(-1)) of a nitroxide spin label by a paramagnetic metal.

77 ful tool in the development of site-directed spin labeling by resolving rotamers of the nitroxide spi

78 l analysis indicated that orientation of the spin label can be determined within <2.1 degrees accurac

79 phyrin triplet state (S = 1) and a nitroxide spin label chemically incorporated into a small helical

80 s and interspin distances were measured to a spin-labeled cobalamin using pulse EPR spectroscopy.

81 Site-directed spin labeling combined with electron paramagnetic resona

82 However, the spin labels commonly employed are highly flexible, which

83 the ones reported by the more standard MTSSL spin label, commonly employed in protein studies.

84 omer, called globulomer, using site-directed spin labeling complemented by other techniques.

85 pin resonance spectroscopy and site-specific spin-labeling confirm that the Tsr HAMP maintains a four

86 s are considered rigid; the position of each spin-label conformer and the structure of each protein c

87 optimizing the positions and populations of spin-label conformers against intradomain paramagnetic r

88 We used circular dichroism and site-directed spin labeling coupled with electron paramagnetic resonan

89 es to calculate their distances to a trio of spin-labeled Cys mutants.

90 one should be cautious in interpretation of spin label data when charged and polar residues in small

91 ophobic residue Phe is labeled, however, the spin-label depth is close to that of the native residue

92 for this regulation, wild-type RyRp and four spin-labeled derivatives were synthesized, each containi

93 We used PC spin labels dipalmitoylphospatidyl-tempo-choline (on the

94 ntermolecular dipolar interactions sensed by spin-labels distributed over the protein surface, we sho

95 flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides a

96 t computational comparison, we find that the spin label does not perturb the signature population of

97 Spin-labeled double-cysteine mutants of VcSiaP were anal

98 Here, we combine spin-labeling double electron-electron resonance (DEER)

99 nstraints (e.g., engineered metal bridges or spin-labels), each treated as an individual molecular fr

100 ometry, circular dichroism and site-directed spin label electron paramagnetic resonance spectroscopy,

101 the C-terminus of EcMscL using site-directed spin labelling electron paramagnetic resonance (SDSL EPR

102 system, which we confirmed by site-directed spin-label electron paramagnetic resonance spectroscopy.

103 In this study, we use site-directed spin-labeling electron paramagnetic resonance spectrosco

104 Site-directed spin-labeling electron paramagnetic resonance spectrosco

105 addition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron r

106 First, the spin-label ensemble is determined by optimizing the posi

107 ack reactive cysteines and that paramagnetic spin labels entering the periplasm are selectively reduc

108 High-pressure site-directed spin-labeling EPR (SDSL-EPR) was developed recently to m

109 These findings, in combination with spin-labeling/EPR spectroscopic measurements in reconsti

110 amate (Glu) and glutamine (Gln) and arterial spin labeling evaluation for rCBF.

111 which was further validated by site-directed spin labeling experiments.

112 NMR and spin-labeling experiments showed that GH5_pMut bound to

113 ace are targeted by using negatively charged spin-labeled fatty acids that display selectivity of int

114 t there exist a number of challenges such as spin-label flexibility, domain dynamics, and overfitting

115 cluding thiol-specific methane thiosulfonate spin label for site-directed spin labeling.

116 dient-recalled echo to assess CMBs, arterial spin labeling for CBF, and T1- and T2-weighted imaging f

117 which serve as models in the search for new spin labels for DEER distance measurement at room temper

118 n healthy individuals (n=23) during arterial spin labeling functional magnetic resonance imaging (fMR

119 Pseudocontinuous arterial spin labeling functional magnetic resonance imaging and

120 Using arterial spin labeling functional magnetic resonance imaging, we

121 tivity, which was assessed by using arterial spin-labeling functional magnetic resonance imaging 4 h

122 and disease parameters, we used an arterial-spin-labeling functional MRI stress paradigm in 36 MS pa

123 EPR spectra of spin-labeled fusion domains also indicated different con

124 Here, we demonstrate that the Gd(III) based spin label Gd-PyMTA is suitable for in-cell EPR.

125 erimentally derived distance measurements of spin-labeled GLIC suggest ELIC is not a good model for t

126 A spirocyclohexyl spin label has been prepared that has longer Tm between

127 Site-directed spin labeling has been employed in this work to address

128 resonance in conjunction with site-directed spin labeling has been used to probe natural conformatio

129 ce (DEER), in conjunction with site-directed spin-labeling, has emerged in the past decade as a power

130 Arterial spin labelling identified no significant changes in regi

131 : (i) reduction resistant Gd(3+) chelates as spin labels, (ii) high frequency (94.9 GHz) for sensitiv

132 Pseudo-continuous arterial spin labeling imaging was used to measure resting region

133 clear magnetic resonance, combining arterial spin-labeling imaging of perfusion, and (31)P-spectrosco

134 ent of 140 amol of the most common nitroxide spin label in a approximately 593 fL liquid cell at ambi

135 s the cavity with side chains, including the spin label in one case; external ligands compete with th

136 S) of Escherichia coli, mutants containing a spin label in the cytosolic or the transmembrane region

137 with the assumption that the position of the spin label in the membrane is close to that of the nativ

138 Site-directed spin labeling in combination with double electron-electr

139 Site-directed spin labeling in combination with EPR is a powerful meth

140 are similar to the WT protein, site-directed spin labeling in solution reveals additional conformatio

141 pin resonance spectroscopy and site-directed spin labeling in what to our knowledge is a new approach

142 , we demonstrate the detection of gadolinium spin labels in an artificial cell membrane under ambient

143 uantum bits, and determining the location of spin labels in biological systems.

144 the instabilities of the standard nitroxide spin labels in the cell environment and the limited sens

145 hort lifetime of the commonly used nitroxide spin labels in the reducing milieu inside a cell.

146 lamin transporter BtuB was overexpressed and spin-labeled in whole cells and outer membranes and inte

147 collagen V mimic (synthesized with nitroxide spin labels) in the active site of the catalytic domain

148 ctions could be detected using site-directed spin labels, indicating that the three helices do not ad

149 EPR showed that a spin label inserted near the N-terminus was weakly immob

150 ation was used to take the local mobility of spin labels into account.

151 Residue-level mobility analysis on spin labels introduced at 14 different positions shows a

152 Nitroxide spin labels, introduced specifically at three individual

153 The spin label is assembled in situ from natural amino acid

154 on in each case indicates that the TAM-based spin label is relatively localized.

155 The spin label is well accommodated in several RNA secondary

156 copy (EPR) in combination with site-directed spin labeling is a very powerful tool to monitor the str

157 copy (PDS) in combination with site-directed spin labeling is unique in providing nanometer-range dis

158 uble resonance (PELDOR), using site-directed spin labeling, is most commonly employed to accurately d

159 EPR spectra were obtained for the spin-labeled ligands both free in solution and attached

160 Here, librational motion of spin-labeled lipid chains in membranous Na,K-ATPase is i

161 In this work spin-labeled lipid molecules (SL-lipids), when used as p

162 r for two imaging modalities-pulsed arterial spin labeling magnetic resonance imaging (PASL-MRI) and

163 Arterial spin labeling magnetic resonance imaging was used to col

164 Arterial spin labelling magnetic resonance imaging recognized reg

165 an older adults (n = 232) underwent arterial spin labelling magnetic resonance imaging to measure reg

166 ced cerebral blood flow measured by arterial spin labelling magnetic resonance imaging, but it is unc

167 halopathy lesions was determined by arterial spin labelling magnetic resonance imaging.

168 antified on a voxelwise basis using arterial spin-labeled magnetic resonance imaging at 3T.

169 c flow velocity was quantified by performing spin labeling measurements as a function of postlabeling

170 RE is confirmed in solution by site-directed spin labeling measurements.

171 pposing sides of the catalytic domain engage spin-labelled membrane mimics.

172 onventional line shapes, similar to multiply spin-labeled membranous Na,K-ATPase below 200 K.

173 In multiply spin-labeled membranous Na,K-ATPase, this heterogeneous

174 Using the site-directed spin labeling method of electron paramagnetic resonance

175 The spin label mobility, intersubunit spin-spin proximity, a

176 in helical dynamics we observed for ensemble spin-label mobility reflected differences in proportions

177 We measured distances between pairs of spin labels monitoring the movement of the nucleotide bi

178 Guided by these parameters, an arterial spin labeling MR imaging approach was adapted to measure

179 922 youths ages 8-22 y imaged using arterial spin labeled MRI as part of the Philadelphia Neurodevelo

180 absolute myocardial blood flow (MBF) using a spin-labeling MRI (SL-MRI) method after transplantation

181 We then used arterial spin-labeling MRI to noninvasively measure CBF and asses

182 ut is supported by two crystal structures of spin-labeled MscS.

183 mation is refined by attaching two different spin labels, MTSL or BSL (bifunctional spin-label) onto

184 as to explore this potential using 38 singly spin-labeled mutants of myoglobin distributed throughout

185 ectron paramagnetic resonance line shapes of spin-labeled mutants suggest several conformational stat

186 Intermolecular distances on four singly spin-labeled mVDAC1 mutants were used to generate a mode

187 this work, the crystal structure of a doubly spin-labeled N8C/K28C mutant of the B1 immunoglobulin-bi

188 Spin labeling nucleic acids at specific sites requires t

189 Using blebbistatin we show that spin-labeled nucleotides bound to myosin have an oriente

190 on-electron double resonance measurements of spin-labeled OAM were used to provide direct evidence fo

191 ce tools that rely on site-specific electron spin labeling of Deltatau187.

192 f monocysteine variants and by site-specific spin labeling of the Q-helix followed by EPR-based inter

193 Site-specific spin labeling of the recombinant protein allowed the mea

194 ncy 236.6 GHz EPR, not previously applied to spin-labeled oligonucleotides, was notably sensitive to

195 We used phosphocholine spin labels on the lipid headgroup and different positio

196 mutants toward acrylodan and the mobility of spin labels on these mutants do not show patterns of an

197 cal and beta-sheet aqueous proteins that are spin-labeled on a single cysteine residue display spin-e

198 Current distance measurements between spin-labels on multimeric protonated proteins using doub

199 erent spin labels, MTSL or BSL (bifunctional spin-label) onto the F or G helices and using DEER (doub

200 We used pulsed continuous arterial spin labeling (pCASL), a perfusion magnetic resonance im

201 The results suggest that the spin labeled peptide H-AP10C(Gd-PyMTA)P10C(Gd-PyMTA)P10-

202 The EPR spectrum of each spin-labeled peptide indicates nanosecond dynamic disord

203 Electron spin resonance on spin-labeled peptides confirms these observations.

204 xamine circle of Willis anatomy and arterial spin labeled perfusion magnetic resonance imaging to mea

205 antified on a voxelwise basis using arterial spin labeled perfusion MRI at 3T.

206 Here, we combined arterial spin labeling perfusion and blood oxygen level-dependent

207 ndividuals with schizophrenia using arterial spin labeling perfusion MRI.

208 ocal leukoencephalopathy lesions by arterial spin labelling perfusion magnetic resonance imaging and

209 ood-oxygenation-level-dependent and arterial-spin-labeling perfusion contrasts to investigate the rel

210 electron-electron resonance measurements on spin-labeled Pgp mutants also show wide distributions co

211 owing to local environmental effects on the spin-label phase memory relaxation time Tm .

212 ects and substantially increase the apparent spin-label phase memory relaxation time, complemented by

213 a decrease of 0.8 nm in the distance between spin labels placed at S48C and S190C upon binding the su

214 shows that rotational motion of a nitroxide spin label, placed at the N-terminal end of the first be

215 oscillations observed for most of the double spin-labeled positions indicate a rather rigid orientati

216 and derivatives thereof using site-directed spin labeling, pressure-resolved double electron-electro

217 onance (EPR) studies using nucleotide analog spin label probes showed that dephosphorylated myosin he

218 Moreover, the signal of spin-labeled protein can be selectively detected in cell

219 ein interface strongly resembles that of the spin-labeled protein side chains, suggesting solvent-med

220 igh resolution field cycling (31)P NMR using spin-labeled protein) are combined with enzyme kinetics

221 irwise P(r) distance distributions in doubly spin labeled proteins.

222 is enables the intracellular biosynthesis of spin-labeled proteins and obviates the need for any chem

223 Continuous wave-ESR of the spin-labeled proteins confirms that broader PDS distance

224 a method was developed for rapidly freezing spin-labeled proteins under pressure to kinetically trap

225 in vivo imaging to distance measurements in spin-labelled proteins.

226 e distance and angle measurements with rigid spin labels provide critical input for the refinement of

227 EER) experiments of nucleic acids with rigid spin labels provide highly accurate distance and orienta

228 three-dimensional pulsed-continuous arterial spin labeling provided measurements of regional cerebral

229 as induced by a protein-attached lanthanide spin label, provided structural restraints for the prote

230 The structure of the spin-labeled Q54R1/L173R1 R125A mutant was solved at 2.1

231 We exploited arterial spin-labeling quantitative perfusion imaging and a newly

232 Moreover, NMR and spin-labeling results from the study of the nucleosome i

233 y functional and monomeric PLB mutant with a spin label rigidly coupled to the backbone of the transm

234 A spin label rotamer library based on a molecular dynamics

235 using circular dichroism (CD), Site-Directed Spin Labeling (SDSL) coupled to EPR spectroscopy, and en

236 Here, using site-directed spin labeling (SDSL) electron paramagnetic resonance (EP

237 Site-directed spin labeling (SDSL) has potential for mapping protein f

238 osecond backbone dynamics with site-directed spin labeling (SDSL) in soluble proteins has been well e

239 In the present study, site-directed spin labeling (SDSL) together with double electron-elect

240 With an arterial spin labeling sequence, three networks were first identi

241 eling by resolving rotamers of the nitroxide spin-label side chain in a variety of alpha-helical envi

242 t room temperature and EPR spectroscopy on a spin-labeled single crystal allows to correlate the stru

243 ed to guide positioning of a small number of spin-labeled single-Cys mutants that coat the entire enz

244 or resonances more than 20 residues from the spin-labeling site.

245 fer by concerted changes in the positions of spin-label sites at the base of the bundle.

246 d protein transitions, the ability of single spin-label sites to detect conformational heterogeneity,

247 olar electron spin resonance spectroscopy of spin-labeled soluble receptors active in cells verify th

248 ctive insights into these processes, but new spin-labeling strategies are needed.

249 binding interface in MHV with site-directed spin labeling studies consistent with a model in which t

250 tracellular loop and partially dissociates a spin-labeled substrate analog.

251 The method relies on sparse spin-labeling, supplemented by deuteration of protein an

252 D2O is determined for stearic acid, n-SASL, spin-labeled systematically at the C-n atoms throughout

253 tly homogeneous broadening are found in both spin-label systems.

254 the method for a model system as well as for spin-labeled T4 lysozyme in explicit water, and show how

255 easured using the pseudo-continuous arterial-spin-labeling technique with background suppression and

256 a tonic pain model with concurrent arterial spin labelling that measures cerebral perfusion related

257 uous wave EPR spectroscopy and site-specific spin labels that directly probed, in essentially physiol

258 ere obtained for the CD using a bifunctional spin label to cross-link SH1-SH2, but the CD was more di

259 Here, we use site-specific spin labeling to demonstrate that relaxation enhancement

260 We generated seven mutants suitable for spin labeling to enable application of pulsed EPR techni

261 Here, we used site-directed spin labeling to map the conformation of a pRNA three-wa

262 e imaging based on pseudocontinuous arterial spin labeling to measure CBF at normocapnia (ie, breathi

263 a placebo-controlled study, we used arterial spin labeling to measure IN-OT-induced changes in restin

264 interest (doping/attachment of paramagnetic spin labels to biomolecules of interest).

265 We have attached spin labels to cysteine mutants on key secondary structu

266 used distance measurements between pairs of spin labels to define the conformational cycle of the Na

267 ite-specifically attached pairs of nitroxide spin labels to monitor changes in the mini TAR DNA stem-

268 We attach nitroxide radicals (i.e., spin labels) to multiple phosphate backbone positions of

269 We used site-directed spin-labeling together with electron spin-resonance line

270 -binding site of SULT1A1-is determined using spin-label triangulation NMR.

271 with a set of (2)H, (13)C, and (15)N nuclear spin-labeled tyrosine substrates.

272 roximity of a strategically placed nitroxide spin-label up to 8 A away.

273 in, as an alternative to the introduction of spin labels via engineered cysteine residues.

274 Conditions for attachment of the spin-label via esterification have been optimized on the

275 performed using velocity-selective arterial spin labeling (VSASL) and 3D image acquisition with whol

276 When spin-labeled WALP24 was inserted in two representative l

277 The paramagnetic nitroxide spin label was attached to Cys residues that were placed

278 thod based on the technique of site-directed spin labeling was developed to experimentally map shapes

279 Continuous arterial spin labeling was interleaved with TMS to directly asses

280 Here, site-directed spin labeling was used to probe the solution structures

281 Arterial spin labelling was used to index resting-state perfusion

282 Using site-directed spin labeling, we demonstrated that the pressure- and te

283 Using arterial spin labeling, we measured resting-state cerebral blood

284 ectroscopy in combination with site-directed spin labeling, we show that familial PD-associated varia

285 mages and perfusion images by using arterial spin labeling were obtained for comparison.

286 Spin labels were attached to cytochrome f, and the relax

287 Pairs of spin labels were introduced at residues selected to trac

288 To detect conformational changes, pairs of spin labels were introduced into arrestin-2 and arrestin

289 The distances between attached spin labels were measured using pulsed electron-electron

290 antiparallel-arranged dimer structures when spin labels were placed into the PCM region.

291 Two rotameric states of the spin-label were resolved at the solvent-exposed alpha-he

292 An R5a nitroxide spin label, which was covalently attached at a specific

293 ic sites requires the covalent attachment of spin labels, which involves rather complicated and labor

294 MRI arterial spin labeling, white matter hyperintensities (WMHs) and

295 we are exploring a triarylmethyl (TAM)-based spin label with a relatively long relaxation time where

296 ptide H-AP10CP10CP10-NH2 was site-directedly spin labeled with Gd-PyMTA at both cysteine moieties.

297 oped an approach that combines site-directed spin labeling with continuous wave and pulsed EPR to inv

298 ance is extended to approximately 40 A using spin labels with long T1, a high-affinity 5-residue Cu(2

299 paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitiv

300 n topology based on the accessibility of the spin label, with the assumption that the position of the