ライフサイエンスコーパス: spindle

1 ferentially attaching to the egg side of the spindle.

2 erly interpret cortical cues that orient the spindle.

3 in sperm DNA within 2 microm of the meiotic spindle.

4 tral microtubules to help orient the mitotic spindle.

5 grow out from the poles of the first mitotic spindle.

6 ng activity of Mklp2 at the anaphase central spindle.

7 sperm DNA from interacting with the meiotic spindle.

8 to the spindle poles and orients the mitotic spindle.

9 naphase until all kinetochores attach to the spindle.

10 mosome motion following biorientation on the spindle.

11 A-dynein complexes that position the mitotic spindle.

12 plex pulls astral microtubules to orient the spindle.

13 gularities of the zona pellucida and meiotic spindle.

14 cell lines is sufficient to induce monopolar spindles.

15 Thr/Tyr kinase (TTK)/monopolar spindle 1 kinase (Mps-1) is a kinase of the spindle asse

16 coherence (SFC) with V1 delta (0.5-4 Hz) and spindle (7-15 Hz) oscillations increased, with neurons m

17 PT in which neurons and glia exhibit mitotic spindle abnormalities, chromosome mis-segregation, and a

18 illations (SO; 0.5-1 Hz) and thalamocortical spindle activity (12-15 Hz) during sleep, and their temp

19 lation intensities neither acutely modulates spindle activity during sleep nor theta activity during

20 cue mechanism that drives substrate-parallel spindle alignment of quasi-diagonal metaphase spindles i

21 Mitotic spindle alignment with the basal or substrate-contacting

22 a role for organelle inheritance in mitosis, spindle alignment, and the choice of daughter progenitor

23 In animals, the mitotic spindle aligns with Par complex polarized fate determina

24 ests that dense, transient crosslinks to the spindle along k-fibers bear the load of chromosome movem

25 Downregulation of PI3K-C2alpha causes spindle alterations, delayed anaphase onset, and aneuplo

26 ults in force imbalance leading to monopolar spindle and chromosome segregation failure.

27 res become infiltrated with benign-appearing spindle and epithelioid cells (LAM cells) that express s

28 The spindle and kinetochore-associated (Ska) protein complex

29 It localizes to microtubules of the central spindle and midbody throughout cytokinesis, at sites dis

30 esumptive cleavage furrow in response to the spindle and myosin.

31 ut not AUG8, is associated with acentrosomal spindle and phragmoplast MT arrays in patterns indisting

32 , modified Bibaum(R), triple leader, slender spindle and Solaxe, were evaluated based on agronomic, q

33 genetic factors significantly contribute to spindle and spectral sleep traits.

34 d for the assembly of the subsequent mitotic spindle and to phosphorylate a microtubule-associated pr

35 cal replay tends to occur during local sleep spindles and down-to-up transitions.

36 ng subcortical-cortical propagation of sleep spindles and their related memory benefits.SIGNIFICANCE

37 s supported the formation of de novo meiotic spindles and, after fertilization with sperm, meiosis co

38 hrough triple coupling of slow oscillations, spindles, and ripples.

39 yperpolarize thalamic cells, thus triggering spindles; and (3) thalamic spindles are focally projecte

40 butes to the spindle pole to ensure faithful spindle architecture.

41 instantaneous firing rates (IFRs) of muscle spindles are associated with characteristics of stretch

42 Misaligned metaphase spindles are believed to result in divisions in which on

43 Sleep spindles are characteristic electroencephalogram (EEG) s

44 , thus triggering spindles; and (3) thalamic spindles are focally projected back to cortex, arriving

45 Using function-separating alleles, live-cell spindle assays, and in vitro biochemical analyses, we sh

46 directs nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation.

47 The spindle assembly checkpoint (SAC) delays mitotic progres

48 Inhibition of the spindle assembly checkpoint (SAC) kinase, Mps1, during M

49 ensure accurate chromosome segregation, the spindle assembly checkpoint (SAC) prevents anaphase unti

50 premature mitotic exit is due to defects in spindle assembly checkpoint (SAC) signaling, such that c

51 ched to the spindle; this is achieved by the Spindle Assembly Checkpoint (SAC) that inhibits the Anap

52 hanosensitive signaling cascade known as the spindle assembly checkpoint (SAC) to detect and signal t

53 titioning during cell division relies on the Spindle Assembly Checkpoint (SAC), a conserved signaling

54 e I causes acceleration of MI, bypass of the spindle assembly checkpoint (SAC), and loss of interchro

55 RIP13 and have substantial impairment of the spindle assembly checkpoint (SAC), leading to a high rat

56 appear to rely on several components of the spindle assembly checkpoint but does require the kinetoc

57 The spindle assembly checkpoint ensures the faithful inherit

58 The spindle assembly checkpoint kinase Mps1 not only inhibit

59 spindle 1 kinase (Mps-1) is a kinase of the spindle assembly checkpoint that controls cell division

60 INPP5E in human and murine cells impairs the spindle assembly checkpoint, centrosome and spindle func

61 be induced in the mouse by inactivating the spindle assembly checkpoint.

62 d load-bearing capacity and silencing of the spindle assembly checkpoint.

63 rther revealed Aire's critical functions for spindle assembly in preimplantation embryos.

64 that the nucleoporin ALADIN participates in spindle assembly in somatic cells, and we have also show

65 Accurate mitotic spindle assembly is critical for mitotic fidelity and or

66 KA-dependent, centrosome-independent mitotic spindle assembly is essential for the survival and proli

67 individual chromosomes, and gross defects in spindle assembly or stability.

68 te centrosomes, thus ensuring robust bipolar spindle assembly.

69 orce required to separate centrosomes during spindle assembly.

70 st a mechanism for how Kif15 rescues bipolar spindle assembly.

71 trix has been proposed to facilitate mitotic spindle assembly.

72 The nucleolar and spindle-associated protein NUSAP1 is a microtubule-bindi

73 terization of a minimal CPC, we suggest that spindle association is important for active Ipl1/Aurora

74 regulated microtubule tyrosination to induce spindle asymmetry and that non-Mendelian segregation dep

75 in conjunction with spindle positioning and spindle asymmetry are key determinants for correct cleav

76 dle, but the molecular mechanisms underlying spindle asymmetry are unknown.

77 determined by the orientation of its mitotic spindle at metaphase.

78 in early mitosis and shuttles to the midzone spindle at mitotic exit.

79 ment, at which point the ability to maintain spindle bipolarity becomes a function of HSET-mediated s

80 We demonstrate that cut7Deltapkl1Delta spindle bipolarity requires the microtubule antiparallel

81 zing to astral MTs, She1 also targets to the spindle, but its role on the spindle remains unknown.

82 some asymmetry between the two sides of the spindle, but the molecular mechanisms underlying spindle

83 lity to cluster centrosomes and form bipolar spindles, but it is not required for division in almost

84 p, permitting the cortex to control thalamic spindling by inducing downstates.

85 ree of degeneration determines whether sleep spindles can promote motor memory consolidation.

86 y downregulated in clinical samples of human spindle cell metaplastic breast carcinoma.

87 null mice it leads to increased incidence of spindle cell sarcomas, including RMS.

88 The spindle checkpoint acts as a mitotic surveillance system

89 The master spindle checkpoint kinase Mps1 senses kinetochore-microt

90 ts (Apc14 and Apc15) regulate association of spindle checkpoint proteins, in the form of the mitotic

91 diffuse outside the confines of the mitotic spindle compartment.

92 We find that spindle compartments in close proximity wait for one ano

93 distances and cellular constrictions between spindle compartments.

94 termined largely by cell morphology and that spindles consistently center themselves in the XY-plane

95 The mitotic spindle consists of microtubules (MTs), which are nuclea

96 whether learning-induced increases in ripple-spindle coupling are necessary for successful memory con

97 t with the hypothesized importance of ripple-spindle coupling in memory consolidation, post-training

98 ontextual fear conditioning increased ripple-spindle coupling in mice.

99 ted this learning-induced increase in ripple-spindle coupling without affecting ripple or spindle inc

100 Spindle defects are not only an impetus of chromosome in

101 , She1 stabilizes interpolar MTs, preventing spindle deformations during movement, and we show that t

102 ith the degree of reduced frontal fast sleep spindle density.

103 The anaphase spindle determines the position of the cytokinesis furro

104 Kar9-dependent orientation of the spindle drove the differential activity of the SPBs in a

105 or lumen continuity by orienting the mitotic spindle during cell division.

106 etween centromeric chromatin and the mitotic spindle during chromosome segregation.

107 uring the cell cycle and assembles a bipolar spindle during mitosis to capture and segregate sister c

108 ion between slow-wave oscillations and sleep spindles during non-rapid-eye-movement (NREM) sleep has

109 Spindles during sleep have been suggested to be importan

110 We find that metaphase and anaphase spindles elongate at the same rate when confined, sugges

111 Our data show that in anaphase, when the spindle elongates, PP1/Repo-Man promotes the accumulatio

112 at astral microtubules are required for such spindle elongation and its maintenance.

113 ormalities including spindle mispositioning, spindle elongation defects, and chromosome segregation d

114 t dynein is required for confinement-induced spindle elongation, and both chemical and physical centr

115 force generation and is sufficient to drive spindle elongation.

116 prematurely stable attachments that restrain spindle elongation.

117 nesin-6 Klp9, which is required for anaphase spindle elongation.

118 The mitotic spindle ensures the faithful segregation of chromosomes.

119 lustered into two poles whose pseudo-bipolar spindles exhibit reduced fidelity of chromosome segregat

120 EMENT Numerous studies have shown that sleep spindle expression is reduced and sleep-dependent motor

121 During cell division, spindle fibers attach to chromosomes at centromeres.

122 e are history-dependent transients of muscle spindle firing that are not uniquely related to muscle l

123 We find that metaphase spindles first undergo a sustained rotation that brings

124 ical isolation and redundancy while allowing spindle fluidity.

125 ities may contribute to disruptions of sleep spindles, focused attention and emotion processing in th

126 e pole delamination and mitotic failure when spindle forces are elevated.

127 of centrosome clustering triggers multipolar spindle formation and mitotic catastrophe, offering an a

128 ls, inhibited mitosis and induced multipolar spindle formation in these cells.

129 Kif15-dependent bipolar spindle formation in vivo requires the C-terminal domain

130 in noncancer-derived cells disturbs neither spindle formation nor mitotic progression.

131 ws search and capture of kinetochores during spindle formation, an important process for accurate chr

132 ule-associated protein important for mitotic spindle formation.

133 activity are sufficient for initial bipolar spindle formation.

134 kemia cells resulted in increased multipolar spindle frequency that correlated with centrosome amplif

135 d active outside the confines of the mitotic spindle from which they are derived.

136 dvance models of force generation needed for spindle function and maintaining integrity.

137 ule cross-linker Shortstop (Shot) in mitotic spindle function in Drosophila Shot localizes to mitotic

138 spindle assembly checkpoint, centrosome and spindle function, and maintenance of chromosomal integri

139 e-movement (NREM) sleep-the thalamo-cortical spindles, hippocampal ripples, and the cortical slow osc

140 tire time course of transient IFRs in muscle spindle Ia afferents during stretch (i.e., lengthening)

141 -bridge dynamics in history-dependent muscle spindle IFRs in passive muscle lengthening conditions re

142 In-phase optogenetic suppression of thalamic spindles impaired hippocampus-dependent memory.

143 tion up-states, but not out-of-phase-induced spindles, improve consolidation of hippocampus-dependent

144 d segregation occurs at the periphery of the spindle in association with interpolar microtubules.

145 ients power the oscillations of the anaphase spindle in budding yeast, but in A. gossypii, this syste

146 Establishing the bipolar spindle in mammalian oocytes after their prolonged arres

147 The proper positioning of mitotic spindle in the single-cell Caenorhabditis elegans embryo

148 Here we characterize spindles in 11,630 individuals aged 4 to 97 years, as a

149 pindle alignment of quasi-diagonal metaphase spindles in anaphase.

150 serial electron tomography of whole mitotic spindles in early C. elegans embryos with live-cell imag

151 We conclude that metaphase spindles in epithelia engage in a stereotyped "dance," t

152 lts suggest a causal role for thalamic sleep spindles in hippocampus-dependent memory consolidation,

153 provide evidence for a physiological link of spindles in the cortex specific to dendrites, the main s

154 spindle coupling without affecting ripple or spindle incidence.

155 Cs) in the Drosophila midgut are replaced by spindle-independent ploidy reduction of cells in the ent

156 ics extracted from the automatically tracked spindles indicate that final spindle position is determi

157 ach in mice, we show here that only thalamic spindles induced in-phase with cortical slow oscillation

158 microtubule attachments may be important for spindle integrity in mitotic cells so that tensile force

159 o apply and sustain large forces to maintain spindle integrity.

160 K-1 prevents expulsion of the entire meiotic spindle into a polar body by negatively regulating the r

161 The spindle is a dynamic structure that changes its architec

162 Asymmetric positioning of the mitotic spindle is a fundamental process responsible for creatin

163 The formation of a bipolar mitotic spindle is an essential process for the equal segregatio

164 The mitotic spindle is composed of dynamic microtubules and associat

165 embly and orientation of the bipolar mitotic spindle is critical to the fidelity of cell division.

166 Here we show that the cut7Deltapkl1Delta spindle is fully competent for chromosome segregation in

167 The position of the mitotic spindle is tightly controlled in animal cells as it dete

168 ls, which typically feature tilted metaphase spindles, lack this anaphase flattening mechanism and as

169 Consistently, MT and spindle length regulators were identified in EML1 pulldo

170 Transient, spindle-like "REM beta tufts" are described in the EEG o

171 w an adult-born neuron with initially simple spindle-like morphology develops into a DGC, consisting

172 ependent microtubules of the meiotic central spindle, located between the segregating chromosomes and

173 we laser-ablate single k-fibers at different spindle locations and in different molecular backgrounds

174 coupling juxtamembrane signaling to mitotic spindle machinery.

175 during DNA repair, and imply that metaphase spindle maintenance is a critical feature of the repair

176 The spindle matrix has been proposed to facilitate mitotic s

177 teracting kinase)-a component of the central spindle matrix-were added.

178 sleep regulation and cognitive functioning, spindles may represent heritable biomarkers of neuropsyc

179 ate interactions between chromosomal DNA and spindle microtubules [1].

180 toring interactions between kinetochores and spindle microtubules and ensuring high-fidelity chromoso

181 tochores, macromolecular complexes that bind spindle microtubules during mitosis.

182 e show here that NUSAP1 localizes to dynamic spindle microtubules in a unique chromosome-centric patt

183 contributes to the localization of Kif15 to spindle microtubules in cells and suppresses motor walki

184 The interaction between chromosomes and spindle microtubules is essential for chromosome segrega

185 Kif18A regulates spindle microtubules through its dual functionality, wit

186 chores, multi-subunit complexes that capture spindle microtubules to promote chromosome segregation d

187 detect and signal the lack of attachment to spindle microtubules, and delay anaphase onset in respon

188 In addition, after attaching to spindle microtubules, the kinetochore generates the forc

189 , targets kinesin-5 and kinesin-12 motors to spindle microtubules.

190 onset until all chromosomes are attached to spindle microtubules.

191 single kinetochore is unattached to mitotic spindle microtubules.

192 nce of kinetochores that are not attached to spindle microtubules.

193 ause the contractile ring ingressed past the spindle midpoint.

194 It was also concentrated on MTs in the spindle midzone and the phragmoplast.

195 delays, and mitotic abnormalities including spindle mispositioning, spindle elongation defects, and

196 d velocity, nor reproduced by current muscle spindle models.

197 nein effector that polarizes dynein-mediated spindle movements in budding yeast.

198 dynein activity along astral MTs and directs spindle movements toward the bud cell.

199 ility increase the frequency and duration of spindle movements, causing positioning errors.

200 ame time, the network of microtubules in the spindle must be able to apply and sustain large forces t

201 is mediated by an anchoring into the entire spindle network and that any direct connections through

202 and downstates cortically, loosely grouping spindle occurrence.

203 similar patterns of overlaps form in central spindles of animal cells, involving the activity of orth

204 Unexpectedly, blocking CenpH did not affect spindle organization and meiotic cell cycle progression

205 work, this function is strictly required for spindle organization, chromosome segregation and cytokin

206 ules are required to rescue focused, bipolar spindle organization.

207 AP21 interactions, Cdc42 activation, mitotic spindle orientation and 3D glandular morphogenesis.

208 k has identified cortical cues that regulate spindle orientation and the division axis [1, 2].

209 Spindle orientation determines the axis of division and

210 ient embryos display defects in apical-basal spindle orientation in delaminated embryonic neuroblasts

211 a cofactor of the p97 AAA ATPase, regulates spindle orientation in mammalian cells by limiting the l

212 In multiple cell types in multiple animals, spindle orientation is controlled by a conserved biologi

213 In animal cells, spindle orientation is regulated by the conserved Galpha

214 Spindle orientation is therefore important for a wide ra

215 of the protein LGN, a core component of the spindle orientation machinery that binds the cytosolic t

216 e, we investigate the mechanisms that couple spindle orientation to polarity during asymmetric cell d

217 Spindle orientation with respect to the substratum is es

218 One deletion impairs mitotic spindle orientation, leading to premature cell cycle exi

219 e pole morphology and a disruption of proper spindle orientation.

220 cycle asynchrony consistently disrupted the spindle orienting mechanism underpinning the invariant c

221 Previous studies indicate that spindles originate thalamically and downstates corticall

222 inally, analysis of the relationship between spindle oscillations and spindle position relative to th

223 In order to decouple ripple-spindle oscillations, here we chemogenetically inhibited

224 mely hippocampal SWRs and cortical delta and spindle oscillations, which is prevalent during sleep.

225 memory consolidation during sleep, the role spindles play in this interaction is elusive.

226 The spindle plays a vital role in accurate chromosome segreg

227 iously unidentified component of the mitotic spindle pole and the centrosome.

228 Cdc5 also localizes to the spindle pole bodies (SPBs) from S phase until the end of

229 (MTOCs), known as centrosomes in animals and spindle pole bodies (SPBs) in fungi, are important for t

230 ganisms such as fungi, centrosomes [known as spindle pole bodies (SPBs)] are essential for cell divis

231 Schizosaccharomyces pombe harbors MTOCs at spindle pole bodies, transient MTOCs in the division pla

232 ecular architecture of the core of the yeast spindle pole body (SPB) by Bayesian integrative structur

233 In this state, Cdc14 targets the spindle pole body (SPB) component Spc110 to counterbalan

234 The spindle pole body (SPB) of budding yeast duplicates once

235 nters (MTOCs; mammalian centrosome and yeast spindle pole body [SPB]) nucleate more astral microtubul

236 in sealing the nuclear envelope in mammals, spindle pole body dynamics in fission yeast, and surveil

237 polarity becomes a function of HSET-mediated spindle pole clustering.

238 tromere and leads to lagging chromosomes and spindle pole defects.

239 portant for centrosome number regulation and spindle pole integrity specifically in mES cells.

240 some alignment at the metaphase plate and in spindle pole integrity.

241 Spindle pole localization of WDR62 and mitotic progressi

242 s, and its knockdown results in an unfocused spindle pole morphology and a disruption of proper spind

243 s find that large cytoplasmic volume affects spindle pole morphology, chromosome alignment, and strin

244 e development of mitotic aster asymmetry and spindle pole movement towards the subdomain of cortical

245 e organization and then redistributes to the spindle pole to ensure faithful spindle architecture.

246 localizes to the cell cortex adjacent to the spindle poles and orients the mitotic spindle.

247 hromosomal missegregation, misorientation of spindle poles and the generation of extra centrosomes, w

248 We find spindle poles are largely dispensable, and in fact act a

249 e more astral microtubules on one of the two spindle poles than the other.

250 tion in Drosophila Shot localizes to mitotic spindle poles, and its knockdown results in an unfocused

251 iple chromosomes associated with one or both spindle poles, causing a significant mitotic delay.

252 embles in an equatorial zone between the two spindle poles.

253 tners attached to microtubules from opposite spindle poles.

254 ather than intrinsic differences between the spindle poles.

255 tically tracked spindles indicate that final spindle position is determined largely by cell morpholog

256 elationship between spindle oscillations and spindle position relative to the cortex reveals an assoc

257 dance," that this dance culminates in proper spindle positioning and orientation, and that completion

258 controlled Myosin flows in conjunction with spindle positioning and spindle asymmetry are key determ

259 ion of astral microtubules during asymmetric spindle positioning has remained elusive.

260 s in the cell, such as vesicle transport and spindle positioning, are mediated by the motor protein c

261 polymerization causes exaggerated asymmetric spindle positioning.

262 ertile and display normal pulling forces and spindle positioning.

263 es such as chromosome attachment and correct spindle positioning.

264 ain of the ER that is involved in asymmetric spindle positioning.

265 We demonstrate that muscle spindle primary afferents in passive muscle fire in dire

266 Sleep spindles promote the consolidation of motor skill memory

267 losum, statistically moderates whether sleep spindles promoted overnight consolidation of motor skill

268 Spindle properties are highly reliable but exhibit disti

269 nter-chromosomal microtubules of the central spindle push chromosomes apart during meiotic anaphase i

270 and synchronised during oscillations in the spindle range in naturally sleeping rodents.

271 targets to the spindle, but its role on the spindle remains unknown.

272 se connections do not limit the timescale of spindle reorganization.

273 itic calcium synchronisation correlates with spindle-rich sleep phases.

274 al cell rounding, which enables unobstructed spindle rotation.

275 ts of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-

276 IMPORTANCE Most of the putative genes in the spindle-shaped archaeal hyperthermophile fuselloviruses

277 nner and outer nuclear layers and within the spindle-shaped cell population in the vanguard in Case 1

278 Filamin condenses short actin filaments into spindle-shaped droplets, or tactoids, with shape dynamic

279 This is especially true for the spindle-shaped viruses of the family Fuselloviridae, whe

280 We classify them as kinetochore (KMTs), spindle (SMTs) or astral microtubules (AMTs) according t

281 a microtubule-binding protein implicated in spindle stability and chromosome segregation.

282 ther, these results show that Cdc14 promotes spindle stability and DSB-SPB tethering during DNA repai

283 is required for the maintenance of metaphase spindle stability.

284 be generated independent of biochemical and spindle structural differences.

285 -galactose and its metabolites disturbed the spindle structure and chromosomal alignment, which was a

286 tu2 on the mitotic spindle to promote proper spindle structure and dynamics.

287 trosomes, the organelles at the poles of the spindle that can persist as microtubule organizing cente

288 til all the chromosomes have attached to the spindle; this is achieved by the Spindle Assembly Checkp

289 echanism and as a consequence maintain their spindle tilt through cytokinesis.

290 that optimally positions Stu2 on the mitotic spindle to promote proper spindle structure and dynamics

291 In budding yeast, dynein moves the mitotic spindle to the predetermined site of cytokinesis by pull

292 transmission between generations by maternal spindle transfer, pronuclear transfer or polar body tran

293 Here, we employ potato spindle tuber viroid (PSTVd) infecting tomato as a syste

294 We show that spindle UM cells can become epithelioid but not vice ver

295 hiasmata and then move back and forth on the spindle until they are bioriented, with the kinetochores

296 entrosome and primary cilia were altered and spindles were found to be abnormally long in HeCo progen

297 roprioceptive sensory neurons and the muscle spindle, which is embedded in the muscle tissue and comp

298 eate from preexisting MTs within the mitotic spindle, which requires the protein TPX2, but the mechan

299 opposite end of the embryo from the meiotic spindle while yolk granules are transported throughout t

300 OCs and contributes to orienting the mitotic spindle within the cell.