ライフサイエンスコーパス: spindle pole

1 er partners TRF1 (at telomeres) and NuMA (at spindle poles).

2 rs of some chromosomes did not extend to the spindle pole.

3 sion to occur close to the membrane-proximal spindle pole.

4 ssociation of each centrosome with a mitotic spindle pole.

5 rms microtubule (MT) attachments to only one spindle pole.

6 tners attached to microtubules from opposite spindle poles.

7 inetochore-MT attachments in the vicinity of spindle poles.

8 o then regroup and merge them into two equal spindle poles.

9 restored by inhibition of Aurora A kinase at spindle poles.

10 ather than intrinsic differences between the spindle poles.

11 kinases Plk1 and Aurora A at centrosomes and spindle poles.

12 rallel architecture that concentrates MTs at spindle poles.

13 .571dupA mutation did not show CKAP2L at the spindle poles.

14 ween microtubules that emanate from opposite spindle poles.

15 g that MEI-1 and ASPM-1 act independently at spindle poles.

16 ched kinetochores along microtubules towards spindle poles.

17 o interpolar spindle microtubules and to the spindle poles.

18 bules (MTs) and slide them apart, toward the spindle poles.

19 p build this bipolar array by separating the spindle poles.

20 is essential for the organization of mitotic spindle poles.

21 errors due to extra centrosomes and mitotic spindle poles.

22 controlled in part through activities at the spindle poles.

23 embles in an equatorial zone between the two spindle poles.

24 Sister kinetochores barely move apart toward spindle poles.

25 moved transiently both toward and away from spindle poles.

26 tic entry and the accumulation of Mud at the spindle poles.

27 ivators ASPM-1 and LIN-5 are concentrated at spindle poles.

28 equally in mitosis through association with spindle poles.

29 pull on microtubules emanating from opposite spindle poles.

30 t, and its signal was pronounced in anaphase spindle poles.

31 required for assembly of acentriolar meiotic spindle poles.

32 localization to spindle microtubules but not spindle poles.

33 cells were highly disorganized with multiple spindle poles.

34 uMA could not efficiently concentrate at the spindle poles.

35 um until the chromosomes are embedded in the spindle poles.

36 e spindle and ultimately toward the opposite spindle poles.

37 g to forces that move chromosomes toward the spindle poles.

38 to dynamic microtubules (MTs) from opposite spindle poles.

39 ents to microtubules emanating from opposite spindle poles.

40 eater than two centrosomes, generating extra spindle poles.

41 ey can be evenly distributed towards the two spindle poles.

42 crotubules (MTs) emanating from the opposing spindle poles.

43 shing mal-oriented chromosome arms away from spindle poles.

44 gle kinetochore binds microtubules from both spindle poles [1], is a major cause of chromosome instab

45 manates from chromatin and diminishes toward spindle poles [6].

46 to mammals, Wts kinase localizes to mitotic spindle poles, a prominent site of Mud localization.

47 that cytoplasmic dynein is required for the spindle pole accumulation of Astrin, and dynein-mediated

48 ting that AMPK is essential for pMRLC(ser19) spindle pole activity.

49 th inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition.

50 ne-fly spermatocytes and inward movements of spindle poles after laser cuts across Potorous tridactyl

51 congression by pushing chromosomes away from spindle poles, although knowledge of the molecular mecha

52 hment of sister kinetochores to the opposite spindle poles (amphitelic attachment).

53 nucleation complex (gammaTC) favors the old spindle pole, an asymmetry inherent to the outer plaque

54 e poles and represent centrosome-independent spindle-pole analogs.

55 which causes chromosomes to move toward the spindle poles (anaphase A).

56 in which chromosomes move toward stationary spindle poles, anaphase B, in which chromosomes move at

57 d the p150(Glued) subunit of dynactin to the spindle pole and cell cortex and of CLIP170 and p150(Glu

58 gle MT (lateral attachment) extending from a spindle pole and is subsequently anchored at the plus en

59 iously unidentified component of the mitotic spindle pole and the centrosome.

60 uous K-fibers connect every kinetochore to a spindle pole and the force for chromosome movement is pr

61 otubule-associated protein that localizes to spindle poles and aids in microtubule stabilization, but

62 ed activation of Plk1 and Aurora A kinase at spindle poles and an impaired localization of MCAK and H

63 Ncd cross-links parallel microtubules at the spindle poles and antiparallel microtubules within the s

64 gamma-TuRC) microtubule-organizing centre at spindle poles and can alter its structure and function.

65 g animal cap mitoses, Kif2a localizes to the spindle poles and centromeres.

66 Asp-CaM interaction alone leads to unfocused spindle poles and centrosome detachment.

67 interpolar microtubules, which span the two spindle poles and contribute to mitotic pole separation

68 activation results in dynein accumulation at spindle poles and dynein-dependent rotation of one spind

69 on resulted in the accumulation of dynein at spindle poles and dynein-dependent spindle rotation with

70 phosphorylated AMPK localizes to the mitotic spindle poles and increases when cells enter mitosis.

71 otic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and tra

72 repeat protein localizing to centrioles and spindle poles and is associated with short stature, onyc

73 for balanced distribution of Astrin between spindle poles and kinetochores.

74 localizes to the cell cortex adjacent to the spindle poles and orients the mitotic spindle.

75 TRIM69, which associates with spindle poles and promotes centrosomal clustering, is es

76 reflect many of the functional properties of spindle poles and represent centrosome-independent spind

77 apparatus (NuMA) complex from cell cortex to spindle poles and show that actin filaments counteract s

78 Phosphorylated Hsp72 concentrates on spindle poles and sites of MT-kinetochore attachment.

79 icrotubules and with LIN-5-ASPM-1 at meiotic spindle poles and that the APC promotes spindle rotation

80 hromosomal missegregation, misorientation of spindle poles and the generation of extra centrosomes, w

81 but delayed the separation of early mitotic spindle poles and the timely mitotic progression.

82 rent models propose that displacement of the spindle poles and/or the activity of kinetochore microtu

83 est can be independent of their kinetochore, spindle pole, and nuclear envelope localization.

84 e bind microtubules oriented toward the same spindle pole, and we find evidence for syntelic kinetoch

85 PSKs are located in the cytoplasm and at the spindle poles, and during telophase and cytokinesis stim

86 tion in Drosophila Shot localizes to mitotic spindle poles, and its knockdown results in an unfocused

87 branous structures, such as DNA repair foci, spindle poles, and RNA granules.

88 spindle pole, indicating the inner core and spindle pole are independent and co-regulated.

89 olonged in the mutants while two acentriolar spindle poles are assembled.

90 We find spindle poles are largely dispensable, and in fact act a

91 codes a protein localized to centrosomes and spindle poles, as well as to the primary cilium.

92 a microtubules accumulated, extra functional spindle poles assembled, and chromosomes frequently segr

93 c spindle through Rab11-dependent control of spindle pole assembly and function.

94 ule severing and ASPM-1 both promote meiotic spindle pole assembly in C. elegans oocytes, whereas the

95 icrotubule-binding structure may mediate the spindle-pole assembly activity of katanin during female

96 d biallelic truncating CSPP1 (centrosome and spindle pole associated protein 1) mutations in 19 JBTS-

97 al analysis and showed that the spindle- and spindle pole-associated clathrin pools are membrane-boun

98 e(19) phosphorylated MRLC (pMRLC(ser19)) and spindle pole-associated pMRLC(ser19) are abolished when

99 ther, these data indicate that AMPK mediates spindle pole-associated pMRLC(ser19) to control spindle

100 at the spindle pole is mutually dependent on spindle pole-associated protein Msd1.

101 Our observations reveal that RED is a spindle pole-associated protein that colocalizes with MA

102 ed to the meiotic spindle, especially to the spindle poles at metaphase, while it was concentrated at

103 erentially adopt shallow beta angles between spindle pole axis and substratum.

104 LARG colocalizes with alpha-tubulin at the spindle poles before localizing to the central spindle.

105 MEN is a signaling cascade that localizes to spindle pole bodies (SPBs) and activates the phosphatase

106 Cdc5 also localizes to the spindle pole bodies (SPBs) from S phase until the end of

107 nt activation, S. pombe Dma1 concentrates at spindle pole bodies (SPBs) in an FHA-dependent manner an

108 (MTOCs), known as centrosomes in animals and spindle pole bodies (SPBs) in fungi, are important for t

109 Tts1 promotes insertion of spindle pole bodies (SPBs) in the NE at the onset of mit

110 Centrosomes, or spindle pole bodies (SPBs) in yeast, are vital mechanica

111 Whereas binding of MEN components to spindle pole bodies (SPBs) is necessary for MEN signalin

112 lizes to the mother cell and associates with spindle pole bodies (SPBs) located in the mother cell to

113 e two nuclei, whereas dynein accumulating at spindle pole bodies (SPBs) may pull MTs nucleated from t

114 enable nucleocytoplasmic transport, and the spindle pole bodies (SPBs) that mediate chromosome segre

115 ation network) and measured their binding to spindle pole bodies (SPBs), the centrosome equivalent of

116 l unexpected structural modifications of the spindle pole bodies (SPBs), the yeast microtubule organi

117 Second, Ipl1 localizes to the spindle pole bodies (SPBs), where it blocks spindle asse

118 At mitosis NIMA-GFP locates to spindle pole bodies (SPBs), which contain Cdk1/cyclin B,

119 ganisms such as fungi, centrosomes [known as spindle pole bodies (SPBs)] are essential for cell divis

120 Yeast centrosomes (called spindle pole bodies [SPBs]) remain cohesive for hours du

121 ation is required for proper localization at spindle pole bodies and the cell division site, E3 ligas

122 At this time, centromeres and spindle pole bodies relocate to the bud neck, explaining

123 Schizosaccharomyces pombe harbors MTOCs at spindle pole bodies, transient MTOCs in the division pla

124 required to recruit the MEN kinase Cdc15 to spindle pole bodies, which is both necessary and suffici

125 tion of Nak1 causes removal of Nak1 from the spindle pole bodies, which may both relieve Nak1 inhibit

126 Most components of the MEN localize to spindle pole bodies.

127 the speed and acceleration of two separating spindle pole bodies.

128 ast, spindle orientation begins with the old spindle pole body (SPB) (from the preceding cell cycle)

129 , while cytoplasmic microtubules vanish, the spindle pole body (SPB) assembles a long and stable mono

130 ecular architecture of the core of the yeast spindle pole body (SPB) by Bayesian integrative structur

131 Mutations in the spindle pole body (SPB) component Cut12 suppress otherwi

132 In this state, Cdc14 targets the spindle pole body (SPB) component Spc110 to counterbalan

133 The fission yeast interphase spindle pole body (SPB) is a bipartite structure in whic

134 (Saccharomyces cerevisiae) the multilayered spindle pole body (SPB) is embedded in the nuclear envel

135 The yeast spindle pole body (SPB) is the functional equivalent of

136 isiae, nuclear pore complexes (NPCs) and the spindle pole body (SPB) must assemble into an intact nuc

137 The spindle pole body (SPB) of budding yeast duplicates once

138 The SIN is assembled at the spindle pole body (SPB) on the scaffold proteins Cdc11 a

139 8 kinesin in CDH1-m11 cells does not promote spindle pole body (SPB) separation.

140 e yeast centrosome-equivalent organelle, the spindle pole body (SPB), and it is involved in multiple

141 ation initiation network (SIN), an essential spindle pole body (SPB)-associated kinase cascade, which

142 ter side of the bud neck and to the daughter spindle pole body (SPB).

143 ions: the nuclear pore complex (NPC) and the spindle pole body (SPB).

144 Cs) such as the animal centrosome and fungal spindle pole body (SPB).

145 iciently suppresses cyclin B-Cdc2 around the spindle pole body (SPB).

146 anchors the septum initiation network to the spindle pole body (SPB, centrosome equivalent) to contro

147 The spindle position checkpoint (SPOC) is a spindle pole body (SPB, equivalent of mammalian centroso

148 nters (MTOCs; mammalian centrosome and yeast spindle pole body [SPB]) nucleate more astral microtubul

149 fective duplication of the yeast centrosome (spindle pole body [SPB]).

150 stent with aggregation of centromeres at the spindle pole body and compartmentalization of individual

151 e1, cytoplasmic microtubules detach from the spindle pole body at high rates.

152 to the nuclear envelope near the site of the spindle pole body during interphase.

153 e strengths of microtubule attachment to the spindle pole body during these stages of the cell cycle.

154 calization and roles for Hei10 in centrosome/spindle pole body dynamics and associated nuclear traffi

155 in sealing the nuclear envelope in mammals, spindle pole body dynamics in fission yeast, and surveil

156 r many years that centromeres cluster at the spindle pole body in fission yeast.

157 anized in the nuclear envelope, known as the spindle pole body in yeast (analogous to the centrosome

158 charged surface patch that is implicated in spindle pole body localization.

159 The spindle pole body of the budding yeast Saccharomyces cer

160 by inactivating centromere attachment to the spindle pole body or changing the position of ribosomal

161 netic nodes around the equator 10 min before spindle pole body separation (cell-cycle time, -10 min)

162 e gamma-tubulin complex to both centrosomal (spindle pole body) and non-centrosomal sites.

163 perhaps by altering its localization to the spindle pole body, and, thus, that gamma-tubulin plays a

164 mplicated three of these genes in centrosome/spindle pole body, centromere, and cohesion function.

165 a diffusion based mechanism, centred on the spindle pole body, for the coordination of DNA replicati

166 ovel antiparallel bundle associated with the spindle pole body, named Q-MT bundle.

167 lication of the yeast centrosome (called the spindle pole body, SPB) is thought to occur through a se

168 DNA replication but unable to duplicate the spindle pole body.

169 identify a unique link between NuA4 and the spindle pole body.

170 hat tethers the gamma-tubulin complex to the spindle pole body.

171 this domain alone is able to localize to the spindle pole body.

172 e gammaTuRC and anchors the gammaTuRC to the spindle pole body.

173 l early mitosis when they co-localize at the spindle pole body.

174 ype region, from its natural location at the spindle-pole body to the immediate vicinity of the nucle

175 stress response; (2) ESCRT factors regulate spindle-pole-body duplication; and (3) a membrane-protei

176 se, FgCdc14-GFP localized to the nucleus and spindle-pole-body.

177 Poleward streams are integrated at spindle poles, but are diverted by any unattached kineto

178 pulsion of type-1-phosphatase (PP1) from the spindle pole by Fin1 (NIMA) kinase ensures switch-like a

179 iple chromosomes associated with one or both spindle poles, causing a significant mitotic delay.

180 n inner core closely aligned with the unique spindle pole (centrocone) holds distant orthologs of the

181 olar spindle could inhibit SAC silencing and spindle pole clustering could promote it-albeit accompan

182 polarity becomes a function of HSET-mediated spindle pole clustering.

183 y shorten in the pole-to-pole axis such that spindle poles contact the outer kinetochore before the s

184 of Aurora A and Plk1 to the mother (oldest) spindle pole contributes to the fidelity of symmetric ce

185 ant increase of multipolar mitoses and other spindle pole defects.

186 tromere and leads to lagging chromosomes and spindle pole defects.

187 We show that proximity to spindle poles destabilizes kinetochore-MTs and that stab

188 m in which kinetochores attached to opposite spindle poles differ in their interactions with MTs and

189 somes at their kinetochores extended through spindle poles, disrupting them.

190 The acentrosomal meiotic spindle poles do not have centrioles and are not anchore

191 is Gravin scaffold accumulates at the mother spindle pole during metaphase.

192 ng microtubule asters that grow out from the spindle poles during anaphase.

193 -like kinase 1 (Plk1) at the centrosomes and spindle poles during G2/M.

194 the chromosomes, with polar microtubules and spindle poles during metaphase through telophase, and pa

195 ial occurs when chromosomes move to opposite spindle poles during mitosis.

196 k1 protein stability and localization to the spindle poles during mitosis.

197 s to cross-link parallel microtubules at the spindle poles during mitosis.

198 phosphorylated Hice1 primarily localizes to spindle poles during prophase to metaphase but gradually

199 Rnq1 does not normally participate in spindle pole dynamics, but it does assemble at the IPOD

200 ghest phosphorylation levels between the two spindle poles, emerges when a cell enters mitosis.

201 ved caspase-related protease separase (extra spindle poles [ESP]) is required for the establishment o

202 The caspase-related protease separase (EXTRA SPINDLE POLES, ESP) plays a major role in chromatid disj

203 tension to promote the coalescence of early spindle pole foci that produces a bipolar structure duri

204 Ciliobrevins also prevent spindle pole focusing, kinetochore-microtubule attachmen

205 e oscillatory movements to and away from the spindle pole for 1-2 h, so we could trap kinetochores mu

206 kpoint functions, centrosome number control, spindle pole formation, Slug expression and satellite RN

207 defective centrosome maturation and aberrant spindle pole formation, thus impairing the formation of

208 which binds importin-alpha) inhibits mitotic spindle pole formation.

209 Overall, the model shows that abnormal spindle-pole formation and its aberrant coordination wit

210 g between kinetochore-spindle attachment and spindle-pole formation in SAC silencing.

211 In PtK2 cells 8-mW trap power stopped the spindle pole from moving toward the equator.

212 ggered simultaneous aMT organization by both spindle poles from the outset and led to symmetric conta

213 s represent APC-independent roles for EB1 in spindle pole function and the regulation of cortical con

214 cellular MTOCs progressively decreased, the spindle pole gradually became more focused, and spindle

215 e fusion protein, which localizes to mitotic spindle poles, has constitutive kinase activity and indu

216 Aurora B kinase (ABK), typically occurs near spindle poles; however, the relevance of this locale is

217 extracts, we show that SSX2IP accumulated at spindle poles in a Dynein-dependent manner and interacte

218 es then separate at the same velocity as the spindle poles in an anaphase B-like movement.

219 a manner consistent with its localization to spindle poles in cells.

220 over, our mathematical model showed that two spindle poles in close proximity do not "search" the ent

221 ites, focusing microtubule minus ends to the spindle poles in early mitosis, and is implicated in mai

222 es excess foci that are competent as mitotic spindle poles in HsSas-6-depleted cells, suggesting that

223 entrosomes are clustered into two functional spindle poles in many cancer cells.

224 ed protein that colocalizes with MAD1 at the spindle poles in metaphase and anaphase.

225 Centrioles promote formation of spindle poles in mitosis and act as basal bodies to asse

226 ence protein, can colocalize with RED at the spindle poles in prometaphase, and their expression can

227 and CENP-E transported chromosomes away from spindle poles in random directions.

228 ly, accumulation of checkpoint components at spindle poles increases markedly only when every kinetoc

229 e inner core amplified along with the unique spindle pole, indicating the inner core and spindle pole

230 portant for centrosome number regulation and spindle pole integrity specifically in mES cells.

231 some alignment at the metaphase plate and in spindle pole integrity.

232 Therefore, stabilization of the centrosome-spindle pole interface by the CEP215-HSET complex could

233 bryos are also defective at coalescing extra spindle poles into a bipolar spindle.

234 Pkl1 localization and function at the spindle pole is mutually dependent on spindle pole-assoc

235 interaction between centrosomes and mitotic spindle poles is important for efficient spindle formati

236 ynein recruitment to the nuclear surface and spindle poles is severely reduced in Lis-1 male germ cel

237 of this organelle in organizing the mitotic spindle poles is well established, its precise contribut

238 s attach to microtubules (MTs) from opposing spindle poles, is the configuration that best ensures eq

239 if2a-depleted oocytes were also defective in spindle pole localization of gamma-tubulin and showed sp

240 Spindle pole localization of WDR62 and mitotic progressi

241 astral microtubules, restores gamma-tubulin spindle pole localization, and generates robust spindles

242 Not only do these data reveal a spindle-pole-localized complex for spindle orientation,

243 Astral microtubules grow out from the two spindle poles, make contact with the cell cortex, and th

244 This suggests a role for Rab11 activity in spindle pole maturation during mitosis.

245 ism for aneuploidy in fission yeast based on spindle pole microtubule defocusing by loss of kinesin-1

246 chromosomes with kinetochores unattached to spindle pole microtubules.

247 s, and its knockdown results in an unfocused spindle pole morphology and a disruption of proper spind

248 s find that large cytoplasmic volume affects spindle pole morphology, chromosome alignment, and strin

249 ghter cells by dynein-dependent differential spindle pole motion in anaphase.

250 Bud6p couples MT growth and shrinkage with spindle pole movement relative to the contact site.

251 e development of mitotic aster asymmetry and spindle pole movement towards the subdomain of cortical

252 itoses that showed the severest asymmetry in spindle pole movement.

253 se-specific cortical blebbing and asymmetric spindle pole movement.

254 Instead, MAPKBP1 is recruited to mitotic spindle poles (MSPs) during the early phases of mitosis

255 of a unique set of centrosome proteins from spindle poles (ninein, Cep215, centriolin).

256 ove at the same velocity as outwardly moving spindle poles, or both.

257 that several MT-RNAs are required for normal spindle pole organization and gamma-tubulin distribution

258 inetochore microtubule rigidity, and also to spindle pole organization by kinesin-5 and dynein.

259 Further, we show that these spindle pole-positioning roles are independent of the CL

260 e are attached to microtubules from opposite spindle poles prior to chromosome segregation at anaphas

261 any poles as assayed by localization of the spindle-pole protein, ASPM-1.

262 of mutations in genes encoding centrosome or spindle pole proteins that cause autosomal recessive pri

263 rotubules, delays mitosis, and redistributes spindle pole proteins.

264 scaffold for this functionally linked set of spindle pole proteins.

265 e microtubules (kMTs), which extend from the spindle pole region to kinetochores assembled upon centr

266 nal NuMA, microtubules lose connection to MI spindle poles, resulting in highly disorganized early sp

267 d the dissociation of gamma-tubulin from the spindle poles, resulting in severely defective spindles

268 This activity limits the extent to which spindle poles separate, leading to transient spindle len

269 ule polymerization are sufficient to promote spindle pole separation and the assembly of bipolar spin

270 w that Kif15 can assume a commanding role in spindle pole separation as a consequence of its mislocal

271 ion in cells with complete versus incomplete spindle pole separation at NEB.

272 We found that cells with incomplete spindle pole separation exhibit higher rates of kinetoch

273 to be achieved through a mechanism in which spindle pole separation forces from plus-end-directed mo

274 ulation of SON leads to severe impairment of spindle pole separation, microtubule dynamics, and genom

275 lar body contractile rings assemble over one spindle pole so that the spindle must move through the c

276 iation without affecting Mud accumulation at spindle poles, suggesting phosphorylation acts as a mole

277 teins colocalize on spindles and move toward spindle poles, suggesting that they form a complex.

278 rient sister chromatids attached to the same spindle pole (syntelic attachment) after nocodazole trea

279 e more astral microtubules on one of the two spindle poles than the other.

280 nd grow dramatically, each forming a nascent spindle pole that nucleates a radial array of microtubul

281 ile ring forms on the cortex adjacent to one spindle pole, then ingresses down the length of the spin

282 onizes the kinesin-13 depolymerase KLP10A at spindle poles, thereby switching off the depolymerizatio

283 ched to microtubules emanating from the same spindle pole, they activate another mechanosensitive mec

284 ow that clathrin remains associated with the spindle poles throughout mitosis and that this clathrin

285 the Golgi-protein p115 partitioned with the spindle poles throughout mitosis.

286 e organization and then redistributes to the spindle pole to ensure faithful spindle architecture.

287 e poles and dynein-dependent rotation of one spindle pole to the cortex, resulting in perpendicular o

288 tachment errors, kinetochore-MTs detach near spindle poles to allow formation of correct attachments.

289 g force on microtubules that extend from the spindle poles to the cell cortex, thereby displacing the

290 l concentrations of p60, like those found on spindle poles, to maintain severing activity while most

291 Once the spindle pole-to-kinetochore contact has been made, the h

292 ces from minus-end-directed motors that pull spindle poles together.

293 e, two to three microtubules extend from the spindle pole toward the cell cortex.

294 Nevertheless, spindle poles were formed after one hour of mitotic rele

295 es revealed that high NuMA levels at mitotic spindle poles were significantly associated with a decre

296 t mechanism that "engages" to push apart the spindle poles when poleward flux is turned off.

297 csi2p localizes to the spindle poles, where it regulates mitotic microtubule dy

298 tubule pairing is sufficient to separate the spindle poles, whereas interpolar microtubules maintain

299 astral microtubule-mediated forces align the spindle poles with cortical cues parallel to the substra

300 nd chromosomes remain in the vicinity of the spindle poles without congression.