ライフサイエンスコーパス: spliced leader

1 ) conveyed via trans-splicing of a universal spliced leader.

2 -5 mRNAs are themselves trans-spliced to SL1 spliced leaders.

3 n-1 RNA transcripts are trans-spliced to the spliced leader 1 and undergo alternative splicing to cod

4 The elements specifically insert in the Spliced leader-1 genes; hence the element has been named

5 the element has been named NeSL-1 (Nematode Spliced Leader-1).

6 TbMTr1 favors the spliced leader 5' sequence, as reflected by a preference

7 A longer spliced leader also can be tolerated but only when prese

8 modifies the first transcribed nucleotide of spliced leader and U1 small nuclear RNAs in the kinetopl

9 ions in the otherwise highly conserved 22-nt spliced leader are tolerated for splicing and post-splic

10 ties and, meanwhile, demonstrate that unique spliced leaders are useful for profiling lineage-specifi

11 g metazoan groups (e.g. nematodes), flatworm spliced leaders are variable in both sequence and length

12 ntly discovered dinoflagellate mRNA-specific spliced leader as a selective primer, we constructed cDN

13 rst characterized in Trypanosoma brucei, the spliced leader-associated (SLA) RNA gene locus has now b

14 SL RNA allowed us previously to identify the spliced leader-associated (SLA) RNA.

15 e-specific non-LTR retrotransposon SLACS, or spliced leader-associated conserved sequence, which inte

16 elomere-associated transposable elements and spliced leader-associated SLACS retrotransposons.

17 RNA contains an identical 39-nucleotide (nt) spliced leader at its 5'-terminus.

18 tor methionine context and the effect of the spliced leader AUG added upstream and out-of-frame with

19 ously suggested that the Schistosoma mansoni spliced leader AUG might contribute a required translati

20 For the nt 28-39 mutated spliced leaders, both the substrate spliced leader RNA a

21 r RNA has the same first four nucleotides as spliced leader, but it receives an m(2,2,7)G cap with hy

22 s is the first report demonstrating that the spliced leader contains critical structural or sequence

23 oth the substrate spliced leader RNA and the spliced leader demonstrated a wild-type methylation patt

24 mutations spanning nucleotides 10-39 of the spliced leader did not affect substrate spliced leader R

25 Undermethylated spliced leaders did not associate efficiently with polys

26 The target specificity of NeSL-1 for the spliced leader exons and the similarity of its structure

27 ubstrate spliced leader RNA and the nt 10-19 spliced leaders found in the poly(A)+ population of RNA;

28 for the cap 4 and/or primary sequence of the spliced leader in translation.

29 structure was present on substrate and mRNA spliced leaders in nt 20-29 mutated exons; nt 20-29 mRNA

30 he 5' end of mRNAs by a m(7)G cap-containing spliced leader is a developing theme in the lower eukary

31 The spliced leader is derived from substrate spliced leader

32 Spliced leader is the majority RNA polymerase II transcr

33 conserved and unique feature of all flatworm spliced leaders is the presence of a 3'-terminal AUG.

34 In trans-splicing, a common exon, the spliced leader, is added to all mRNAs from a small RNA.

35 function of SL1, the major C. elegans trans-spliced leader, is unknown, SL1 RNA, which contains this

36 Interestingly, the spliced leader itself can be dramatically altered, such

37 site on a small RNA of uniform sequence (the spliced leader or SL RNA) has allowed us to characterize

38 rtional to the tagged:wild type ratio in the spliced leader precursor population.

39 This multiplex assay is targeted to the spliced leader RNA (mini-exon) gene repeats of these org

40 The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorh

41 Recombinant TbCgm1 transfers the GMP to spliced leader RNA (SL RNA) via a covalent enzyme-GMP in

42 We report that in Leishmania tarentolae spliced leader RNA 5' modification is influenced by the

43 The spliced leader is derived from substrate spliced leader RNA and joined to pre-mRNA by trans-splic

44 tion was seen between the nt 10-19 substrate spliced leader RNA and the nt 10-19 spliced leaders foun

45 mutated spliced leaders, both the substrate spliced leader RNA and the spliced leader demonstrated a

46 ls for the 5' half of Caenorhabditis elegans spliced leader RNA by comparison of the two-dimensional

47 The spliced leader RNA gene (SL RNA) repeat is present in la

48 sion in these protists, we have investigated spliced leader RNA gene transcription.

49 sable for RNA polymerase II transcription of spliced leader RNA genes (SLRNAs).

50 acidic repetitive genes, Pol II-transcribed spliced leader RNA genes, and Pol III-transcribed U-snRN

51 In Leishmania tarentolae the precursor spliced leader RNA is 96 nucleotides, with a 39-nucleoti

52 A pathway for Leishmania spliced leader RNA processing and maturation is proposed

53 Regulation of spliced leader RNA synthesis is controlled by a triparti

54 Spliced leader RNA transcription is essential for cell v

55 the spliced leader did not affect substrate spliced leader RNA transcription or trans-splicing in Le

56 The precursor spliced leader RNA was tested for trans-splicing functio

57 es, which encode the trans splicing-specific spliced leader RNA, suggests that trypanosomatids assemb

58 stent with its essential role in the Ascaris spliced leader RNA, whereas in Leptomonas mutation of th

59 es and nematodes has been characterized as a spliced leader RNA-facilitated reaction; in contrast, it

60 tes within the first four nucleosides on the spliced leader RNA.

61 a 39-nucleotide-long sequence donated by the spliced leader RNA.

62 sms that have a trans-splicing reaction from spliced leader RNA.

63 is and T. muris operons are trans-spliced to spliced leader RNAs, and we are able to detect polycistr

64 ion, whereas addition of either a TMG-cap or spliced leader sequence alone decreased reporter activit

65 action and an oligonucleotide comprising the spliced leader sequence in the second strand reaction.

66 The trans-spliced leader sequence on mRNAs reduces Dcp2 activity a

67 hat BmGMF is trans-spliced with the nematode spliced leader sequence SL1 and is expressed in microfil

68 lation system, we found that the TMG-cap and spliced leader sequence synergistically collaborate to p

69 e SL1 RNA promoter partly resides within the spliced leader sequence.

70 anscripts, including splice junctions, trans-spliced leader sequences, and polyadenylation tracts.

71 luding occurrence and length distribution of spliced leader sequences, the functional landscape of en

72 everal other lower eukaryotic phyla involves spliced leader (SL) addition trans-splicing.

73 Addition of a 39-nucleotide (nt) spliced leader (SL) by trans splicing is a basic require

74 element responsible for transcription of the spliced leader (SL) gene of Trypanosoma cruzi was identi

75 Through trans-splicing of a 39-nt spliced leader (SL) onto each protein-coding transcript,

76 rasite Trypanosoma brucei, the small nuclear spliced leader (SL) RNA and the large rRNAs are key mole

77 ypanosomes, the m7G cap is restricted to the spliced leader (SL) RNA and the precursors of U2, U3, an

78 scription preinitiation complex (PIC) at the spliced leader (SL) RNA gene (SLRNA) promoter.

79 d, transgenic worms are generated in which a spliced leader (SL) RNA gene is fused with a sequence ta

80 uence, which integrates exclusively into the spliced leader (SL) RNA genes.

81 We report the first data that the spliced leader (SL) RNA is a more specific marker for cu

82 n all trypanosomatids, trans splicing of the spliced leader (SL) RNA is a required step in the matura

83 Spliced leader (SL) RNA trans splicing adds a trimethylg

84 Spliced leader (SL) RNA trans-splicing contributes the 5

85 ting RNA polymerase II-mediated synthesis of spliced leader (SL) RNA, a trans splicing substrate and

86 ction of mRNA relies on the synthesis of the spliced leader (SL) RNA.

87 The two spliced leader (SL) RNAs (SL-A and -B) contain splice do

88 Both the cap structure and the spliced leader (SL) sequence affect levels of A. suum eI

89 transcriptional mechanism whereby a specific Spliced Leader (SL) sequence is added to the 5'end of ea

90 ediated through trans-splicing of the capped spliced leader (SL) sequence of the SL RNA onto the 5' e

91 This short 39 nt m(7)G-capped RNA, the spliced leader (SL) sequence, is expressed as an approxi

92 ypanosomes involves the addition of a common spliced leader (SL) sequence, which is derived from a sm

93 kinetoplastid flagellates trans-splicing of spliced leader (SL) to polycistronic precursors conveys

94 served that CRK9 silencing led to a block of spliced leader (SL) trans splicing, an essential step in

95 In trypanosomes, mRNAs are processed by spliced leader (SL) trans splicing, in which a capped SL

96 Metazoan spliced leader (SL) trans-splicing generates mRNAs with

97 Spliced leader (SL) trans-splicing in Caenorhabditis ele

98 Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods.

99 Spliced leader (SL) trans-splicing is a biological pheno

100 Spliced leader (SL) trans-splicing is a critical element

101 toplastid flagellates attach a 39-nucleotide spliced leader (SL) upstream of protein-coding regions i

102 ced with the addition of the 22-nt conserved spliced leader (SL), DCCGUAGCCAUUUUGGCUCAAG (D = U, A, o

103 mpanied by trans-splicing with a specialized spliced leader (SL), SL2.

104 All trypanosome mRNAs have a spliced leader (SL).

105 Trypanosoma cruzi, and Leishmania spp., the spliced-leader (SL) RNA is a key molecule in gene expres

106 ide RNA derived from a short transcript, the spliced-leader (SL) RNA.

107 Ten different spliced-leader (SL) sequences were found attached to T20

108 Our data suggest that operons and "spliced leader" (SL) trans-splicing predate the radiatio

109 Of the two spliced leaders, SL1 is trans spliced to the 5' ends of

110 trans splicing, generally to the specialized spliced leader SL2, at the 5' ends of the downstream gen

111 products are trans spliced to a specialized spliced leader, SL2.

112 ins must deal with two populations of mRNAs, spliced leader trans-spliced mRNAs with a trimethylguano

113 Spliced leader trans-splicing is an mRNA maturation proc

114 d evidence that another function of flatworm spliced leader trans-splicing is to provide some recipie

115 methylguanosine (m(2,2,7)G) cap derived from spliced leader trans-splicing.

116 ng adds a capped 39-nucleotide mini-exon, or spliced leader transcript, to the 5' end of the main cod

117 Because deg-3 cDNAs contain the SL2 trans-spliced leader, we suggested that deg-3 was transcribed

118 association of mRNAs, which possess mutated spliced leaders, with polysomes.