ライフサイエンスコーパス: spotting

1 n was achieved by using a robotic system for spotting.

2 iotinylated ligands than is required for pin spotting.

3 assays, most approaches use pins for contact spotting.

4 spectively, using the polypropylene tips for spotting.

5 the surface and the size of the tip used for spotting.

6 he steel tips of drafting pens were used for spotting.

7 better than that obtained with manual pipet spotting.

8 epending on the length of dsDNA) after dsDNA spotting.

9 She experienced vaginal spotting 2 years earlier because of an endometrial polyp

10 DBS were created by spotting 50-mul aliquots of whole blood on 903 filter pa

11 Our method involves spotting a 3 muL blood sample on a paper surface where g

12 Microarrays were printed by spotting a library of 7680 N-substituted oligoglycines (

13 By virtue of using a slide for multi-samples spotting and a flatbed scanner for signal-read out acqui

14 us for this mutation (sl) exhibit coat color spotting and congenital intestinal aganglionosis.

15 o a DNA microarray design showing successful spotting and hybridization on whole microarray slides.

16 and the blocking steps conducted during DNA spotting and hybridization.

17 rature indicates vast inconsistencies in the spotting and processing procedures.

18 In this study, spotting and processing protocols were optimized for ald

19 eparate protein expression, purification and spotting, and also overcomes the problem of long-term fu

20 g this automation suite, sample preparation, spotting, and analysis of 100 samples ranging from 12 to

21 elf-reported bleeding patterns, incidence of spotting, and associations with reproductive hormones am

22 tivity, abnormal gait, deafness, white belly spotting, and hypoplasia of Mullerian duct derivatives.

23 ve incorporated a matrix-diluting and liquid-spotting automated work station and a DE MALDI mass spec

24 tochemicals in plant extract samples using a spotting automatic system, mobile phone camera and a com

25 We examined MEF by spotting biotinylated, singly-labeled 23 bp DNAs onto av

26 r detection of blood groups was developed by spotting blood to antibodies absorbed to paper and elute

27 ropyl) dimethylammonia]-1-propane sulfonate) spotting buffer, 24-h postspotting reaction at 100% rela

28 examined included oligo concentration in the spotting buffer, composition of the spotting buffer, pos

29 n in the spotting buffer, composition of the spotting buffer, postspotting "curing" conditions, and p

30 ogressive piebaldism, exhibit dominant white spotting but show no evidence of progressive depigmentat

31 rvival of these cells in vivo, because white spotting (c-kit(W/W)) mice, carrying a natural inactivat

32 the cytokine array membranes were created by spotting capture antibodies onto the membranes.

33 00 pg/mL of dibiotinylated NPY and 0.1 mg/mL spotting capture antibody were found to offer the best p

34 ackground binding of proteins is overcome by spotting carbohydrates through a protein-resistant overl

35 f fabricating oligonucleotide microarrays by spotting commercial presynthesized 65-mers with 5' amine

36 onucleotides, spot and background intensity, spotting concentration and blocking chemistry.

37 ncement was found to be dependent on the DNA spotting concentration: below approximately 12.5 muM, ME

38 Fusarium head blight, leaf spotting diseases, and, more recently, wheat blast (in S

39 ying the piebald mutation exhibit white coat spotting due to the regional absence of neural crest-der

40 It constitutes a very useful tool for spotting essential functions in plant metabolism and to

41 of limiting factors, especially for in-house spotting facilities.

42 eature of the k10(C3H) allele and the dorsal spotting feature of K10(Mayer) allele.

43 paration greatly speeds processing while the spotting function allows for precise sample placement on

44 susceptibility gene 2 (Fv2), dominant white spotting gene (W), and Steel gene (Sl), regulate the ini

45 g ligands using conventional micro-array pin spotting have hindered the application of surface plasmo

46 e have used the varied expressivity of white spotting (hypopigmentation) observed in intrasubspecific

47 ralized hypopigmentation and localized white-spotting in mice, with a lack of pigment on the belly, t

48 ing previous observations that the degree of spotting in piebald mice is dependent on the amount of E

49 tion, suggesting that the color dilution and spotting in vivo may result from reduced numbers of mela

50 The transgene has no effect on coat color spotting, indicating the critical time for EDNRB express

51 ntly in human piebaldism and mouse "dominant spotting." Interestingly, all of the 14 known missense m

52 Spotting lethal (sl) rats carry a naturally occurring de

53 occurring null mutation of the EDNRB gene in spotting lethal (sl) rats, which exhibit aganglionic meg

54 The spotting lethal rat, a naturally occurring rodent model

55 u568_Leu586del) was identified in extra-toes spotting-like (Xsl), an allele of Xs(J).

56 a naturally occurring mutation in the white spotting locus that causes reduced c-Kit receptor expres

57 neous mouse mutation occurring at the lethal spotting (ls) locus.

58 trix can also be applied using our system by spotting matrix droplets on the microarray in a separate

59 Adamts20 mutations cause the mouse white-spotting mutant belted (bt), whereas Adamts9 is essentia

60 (mnlt), a second hypopigmentation and white-spotting mutation identified on the C57BL/6J background,

61 d Ls/Ls mice (mice homozygous for the lethal spotting mutation that develop fetal megacolon after aga

62 nt cell development that are associated with spotting mutations in mice include members of several st

63 om those described for other classical white-spotting mutations.

64 th the corresponding alkali hydroxide before spotting of analyte.

65 The simple spotting of dye solution followed by tensile drawing of

66 (300 microm in diameter) allows high-density spotting of lentivirus, permitting thousands of distinct

67 for genotyping by SPC-SBE and allows direct spotting of sample for rapid analysis by matrix-assisted

68 ing a click reaction after precise nanolitre spotting of specific hexynyl-terminated DNA capture prob

69 ptions of a dilution of coat color and white spotting of the belly and extremities, suggesting a deve

70 abricate SLB microarrays from direct robotic spotting of vesicle solutions.

71 The color and spotting of village weaver eggs in Africa are highly con

72 DNA microarrays produced by deposition (or 'spotting')of a single long oligonucleotide probe for eac

73 TOF MS system, including density of organism spotting on a stainless steel target plate and the direc

74 se purple color, and the fruit showed purple spotting on the epidermis and pericarp.

75 lethality, with viable mice exhibiting white spotting on their ventral surface.

76 ackground and is exacerbated by the dominant spotting oncogene allele KitW-42J.

77 rt that a point mutation, the dominant white spotting oncogene allele, Kit(W-42J), exacerbates XY(DOM

78 ptic peptide mixtures prior to desalting and spotting onto MALDI-TOF plates.

79 Physiological leaf spotting, or flecking, is a mild-lesion phenotype observ

80 urine leakage on filter paper (4-fold higher spotting, P<0.01; 2.5-fold higher urine area percentage,

81 The appaloosa coat spotting pattern in horses is caused by a single incompl

82 Symptoms related to HRT (breast tenderness, spotting, pelvic discomfort, and mood changes) were mild

83 tured microarray under optimized conditions (spotting pH buffer, surface chemistry, blocking procedur

84 nsgene induction in skin resulted in a white spotting phenotype due to somatic ORFeus-mediated mutati

85 The spotting pin encloses the monomer-template protein cockt

86 Reliable test systems for spotting priming-inducing chemistry, however, are rare.

87 recently investigated ways of improving the spotting process.

88 that the microfluidic printer is capable of spotting proteins that are dilute (<0.1 microg/ml) and c

89 and a center-dependent benefit for duplicate spotting (range, 2 to 8.7%).

90 have both decreased, as has the incidence of spotting, relative to the source populations in Africa.

91 hocyanin expression (C or G) and anthocyanin spotting (Rs)] were linked to RAPD loci, but only six of

92 with mendelian inheritance: the major white spotting (S) locus and the hair ridge in Rhodesian ridge

93 Robotic spotting still poses numerous technical challenges.

94 (ii) the battery of measures explored allows spotting strengths in methods that may not be 'best' ove

95 rays, DNA microarrays were also developed by spotting streptavidin-biotinylated oligonucleotide conju

96 ensor chips using copoly(DMA-NAS-MAPS) and a spotting technique, as well as the specificity of their

97 microfluidic channels and also conventional spotting techniques.

98 reduced compared to standard continuous flow spotting technologies as well as in comparison to a conv

99 a modification to our continuous-flow micro-spotting technology that improves ligand deposition.

100 These improvements in spotting technology will help to expand the applications

101 ipids from a kinase assay can be isolated by spotting the crude reaction on a nitrocellulose membrane

102 The method is aimed at spotting the differences between two GCxGC-MS injections

103 inversely proportional to the likelihood of spotting the ends of parallel fibers.

104 can be clearly observed by the naked eye by spotting the resulting sensor solution onto an alumina T

105 After simply spotting the sample onto the electrodes, physisorbed ana

106 This enables rapid cleanup by simply spotting the sample onto the modified DIOS target and re

107 d to dark purple or blue is enhanced through spotting the solution onto a C18 reversed-phase thin-lay

108 Following spotting, the chip was assembled with a cover plate and

109 neration characterized clinically by retinal spotting throughout the fundus and late onset retinal de

110 g electrospotting both the gold chip and the spotting tip are electrically connected to a potentiosta

111 cancer risk using germ cell-deficient white-spotting variant (Wv) mice, incorporating oncogenic muta

112 coded in close proximity at the murine white spotting (W) and patch (Ph) loci.

113 ceptor, c-kit, encoded by the dominant white spotting (W) gene.

114 Mice carrying certain mutations in the white spotting (W) locus (ie, c-kit) exhibit reduced c-kit tyr

115 Finally, mice having mutations in the white spotting (W) locus, which encodes the SCF-receptor (c-ki

116 mouse strains, Steel (Sl) and dominant white spotting (W), to determine if stem cells from an inferti

117 ng these mutants to be termed dominant White spotting (W).

118 kit receptor tyrosine kinase (dominant white spotting, W).

119 he incidence of breakthrough bleeding and/or spotting was significantly higher only in the first 2 cy

120 Manual spotting was used in this report.

121 eral amplification of BAC clone DNA prior to spotting, we designed a set of three novel degenerate ol

122 n is observed only within regions of retinal spotting, which is seen predominantly in the inferior na

123 The extra-toes spotting (Xs) mouse phenotype manifests anterior polydac