ライフサイエンスコーパス: sputum

1 quences associated with XDR-TB directly from sputum.

2 aphy, spirometry, and examination of induced sputum.

3 26% of MTBDRplus tests performed directly on sputum.

4 enotypes according to cell counts in induced sputum.

5 same strain isolated from their PEG tube and sputum.

6 00 CFU of Mycobacterium tuberculosis in 1 ml sputum.

7 Five had MABSC in their sputum.

8 gation was required in 59% cases with scanty sputum.

9 ynx specimens and for mL-gram of protein for sputum.

10 els and the eosinophil percentage of induced sputum.

11 ed NPSs and NPAs; or paired NPSs and induced sputum.

12 most associated with high quality of induced sputum.

13 arameters and performed an omics analysis of sputum.

14 ossing threshold (CT) values were earlier in sputum.

15 -positive participants with culture-positive sputum (13%, 6.4 to 21); and 88% and 83%, respectively,

16 dentified in nasal brushings (5 signatures), sputum (3 signatures), and endobronchial brushings (6 si

17 s ratio [OR] 1.95, 95% CI 0.96-3.95; p<0.1), sputum (3.15, 1.39-7.13; p<0.05), shortness of breath (1

18 s all 462 participants with culture-positive sputum (5.4%, 3.3 to 8.0).

19 Sputum (96%) or endotracheal aspirate (4%) specimens wer

20 d did not improve the diagnostic accuracy of sputum AFB.

21 We sought to compare sputum airway inflammatory phenotypes of African America

22 Sputum analysis in asthmatic patients is used to define

23 ion of inflammatory asthma phenotypes, using sputum analysis, has proven its value in diagnosis and d

24 However due to technical limitations of sputum analysis, there is a strong need for fast and non

25 is of cell pellets, and SOMAscan analysis of sputum analytes were performed.

26 with the detection limits 10(3)cfu/mL TB in sputum and 10(2)cfu/mL Ab in blood within 2h after sampl

27 We present data demonstrating that sputum and aerosol specimens measure 2 related but diffe

28 that a high mucin concentration produces the sputum and disease progression that are characteristic o

29 Two had an identical MABSC strain in their sputum and gastric juice and one had the same strain iso

30 hitis pathophysiologic cascade that produces sputum and mediates disease severity.

31 Sputum and plasma MMP concentrations were quantified by

32 rophil biomarkers were quantified in soluble sputum and serum from patients with COPD during periods

33 ve pulmonary exacerbations, cough, increased sputum, and haemoptysis.

34 h, as evident by the concomitant presence of sputum anti-EPX and anti-nuclear antibodies of the IgG s

35 ned to explore total mucin concentrations in sputum as a diagnostic biomarker and therapeutic target

36 are in the reaction, and efficiently remove sputum associated inhibitors from this large sample.

37 ma that can be identified by the presence of sputum autoantibodies against EPX and autologous cellula

38 was higher among participants who had higher sputum bacillary load (P < .01).

39 We measured lung function, sputum bacterial content, and inflammation, and obtained

40 is from Cape Town, South Africa, underwent a sputum-based liquid culture and Xpert.

41 ing in an urgent unmet need for a rapid, non-sputum-based quantitative test to detect active Mycobact

42 ly important in eosinophilic asthma and that sputum basophil assessment could be a useful additional

43 Sputum basophil numbers are increased in allergic asthma

44 was a trend (P=0.08) towards a reduction in sputum basophils following increased inhaled corticoster

45 Target product profiles (TPPs) for a non-sputum biomarker test to diagnose active TB for treatmen

46 New non-sputum biomarker tests for active tuberculosis (TB) diag

47 eal/oropharyngeal (NP/OP) swabs, and induced sputum by culture and PCR.

48 Bacterial detection in induced sputum by PCR decreased 7% for exposed compared to nonex

49 According to induced sputum cell count, four different asthma phenotypes have

50 tionships between gene expression levels and sputum cell differentials or measures of pulmonary funct

51 synthetase (OAS) and viperin in unstimulated sputum cells in 57 asthmatics (including 16 mild, 19 mod

52 FPR2/ALXR expression was reduced in induced sputum cells of children with SA compared with that seen

53 hensive flow cytometry and RNA sequencing of sputum cells suggest basophils and mast cells, not ILC2s

54 FN-beta, IFN-lambda1/IL-29 and ISGs in their sputum cells that may reflect ongoing innate immune acti

55 Sputum centrifugation may increase the yield of Xpert MT

56 meters, activation of blood granulocytes and sputum characteristics were assessed in 115 adult patien

57 Pooled sputum collection increased tuberculosis diagnosis by mi

58 analysis to investigate whether non-invasive sputum collection methods in people aged at least 12 yea

59 herefore aimed to investigate the effects of sputum collection methods on tuberculosis diagnosis.

60 The relative merits of sputum collection methods to improve tuberculosis diagno

61 Brief, on-demand spot sputum collection was the main reference standard.

62 Providing instructions to the patient before sputum collection, during observed collection, or togeth

63 evealed that both pooled and instructed spot sputum collections were similarly effective techniques f

64 a multidrug regimen produced improvements in sputum conversion and 6-minute-walk distance versus plac

65 Other endpoints included sputum conversion, 6-minute-walk distance, and adverse e

66 ood cell counts and, in a subset, acceptable sputum counts.

67 LAI group demonstrated at least one negative sputum culture (14 [32%] of 44 vs. 4 [9%] of 45; P = 0.0

68 The primary outcome was time to sputum culture conversion (tSCC).

69 Adjunctive vitamin D3 accelerated sputum culture conversion in patients with one or more m

70 Vitamin D3 did not influence time to sputum culture conversion in the study population overal

71 /L; P < 0.001) but did not influence time to sputum culture conversion overall (adjusted hazard ratio

72 on-dependent antagonism that reduces 2-month sputum culture conversion.

73 Sputum culture for pre-migration screening and active re

74 relation between their CT features and their sputum culture results.

75 UAVs on microbiological specimens, blood and sputum culture specimens were seeded with usual pathogen

76 ed pneumococcal if either sputum Gram stain, sputum culture, blood culture, or the immunochromatograp

77 trospective cohort study among patients with sputum culture-positive tuberculosis was performed.

78 microbiologically confirmed with a positive sputum culture.

79 ximately 20% reduction in yield from induced sputum culture.

80 Demographics, clinical characteristics, and sputum cytology after sputum induction were examined.

81 NP/OP specimens with matching high-quality sputum (defined as </=10 epithelial cells/low-power fiel

82 The persistence of eosinophils in sputum despite high doses of corticosteroids indicates d

83 In contrast to sputum diagnostics, urine Xpert and urine-lipoarabinoman

84 Collecting early morning sputum did not significantly increase diagnostic perform

85 nts with smear-negative and culture-positive sputum (difference of 17%, 95% CI 10 to 24); 90% and 77%

86 ain BCG DNA and to combined culture-negative sputum DNA and BCG DNA.

87 Desmosine was correlated with sputum elastase (r = 0.42; P < 0.0001) and was associate

88 Sputum elastase activity increased at exacerbations (P =

89 During a 3-year follow-up, elevated sputum elastase activity was associated with a higher fr

90 Sputum elastase activity was independently associated wi

91 of detection of culture, process >/=1 ml of sputum ensuring sufficient number of MTB are in the reac

92 cant treatment effect on mature eosinophils, sputum EoP numbers or the prednisone maintenance dose.

93 We used a sputum eosinophil 2% cut point to define subjects with e

94 tratified patients on the basis of blood and sputum eosinophil concentrations and compared their demo

95 eosinophil concentrations reliably predicted sputum eosinophil concentrations.

96 Using either a PC20 </=16 mg/mL or a sputum eosinophil count >/=1% increased the sensitivity

97 A FeNO level >/=25 ppb and a sputum eosinophil count >/=2% provided lower sensitivity

98 aseline NSBH despite a positive SIC showed a sputum eosinophil count >/=2%, a FeNO level >/=25 ppb, o

99 haled nitric oxide values in relationship to sputum eosinophil counts (>2%), as well as to determine

100 haled nitric oxide levels (<24 ppb), and (4) sputum eosinophil counts (<2.5%).

101 owed a weak but significant association with sputum eosinophil counts (receiver operating characteris

102 dictive values of NSBH, and FeNO, as well as sputum eosinophil counts assessed at baseline of the SIC

103 sistent moderate-to-severe asthma and raised sputum eosinophil counts despite inhaled corticosteroid

104 approximately 0, including correlations with sputum eosinophil counts.

105 significantly predicted the presence of high sputum eosinophil counts.

106 ment were more common in the high versus low sputum eosinophil group (p=0.002).

107 edian FEV1 percentage predicted than the low sputum eosinophil group both before (65.7% [IQR 51.8-81.

108 The high sputum eosinophil group had significantly lower median F

109 apping were significantly higher in the high sputum eosinophil group than the low sputum eosinophil g

110 he high sputum eosinophil group than the low sputum eosinophil group.

111 The primary outcome was the change in sputum eosinophil percentage from baseline to 12 weeks a

112 inophil peroxidase (EPX) levels with induced sputum eosinophil percentage in 10 adults with poorly co

113 nts were eligible for stratification by mean sputum eosinophil percentage: 656 with low (<1.25%) and

114 th low (<1.25%) and 171 with high (>/=1.25%) sputum eosinophil percentages.

115 study was to compare nasal, pharyngeal, and sputum eosinophil peroxidase (EPX) levels with induced s

116 currently available inflammatory biomarkers sputum eosinophilia and fractional exhaled nitric oxide

117 ytes, (ii) compare its diagnostic value with sputum eosinophilia as gold standard and (iii) validate

118 e sensitivity and specificity for predicting sputum eosinophilia in the 3 weight groups.

119 asthma clusters (T2, T3, and T4) had higher sputum eosinophilia than cluster T1, with no differences

120 l-leucyl phenylalanine was found to identify sputum eosinophilia with 90.5% sensitivity and 91.5% spe

121 Periostin did not identify blood or sputum eosinophilia, even after stratification for total

122 ils >/= 76%), while eosinophilic asthmatics (sputum eosinophils >/= 3%) did not differ from healthy s

123 s, basophils were positively correlated with sputum eosinophils (r=0.54; P<0.005) and inversely with

124 roup 3 patients showed the highest levels of sputum eosinophils and had a high body mass index.

125 d inflammatory features of asthma, including sputum eosinophils and mucins, as well as acute airway r

126 bo, gammaT notably reduced pre-LPS challenge sputum eosinophils and mucins, including mucin 5AC and r

127 Adding the assessment of FeNO level and sputum eosinophils to NSBH improves the identification o

128 nge of COPD severity, high concentrations of sputum eosinophils were a better biomarker than high con

129 g fractional exhaled nitric oxide (FeNO) and sputum eosinophils would be useful adjuncts to the measu

130 ct of 1200 mg of gammaT daily for 14 days on sputum eosinophils, mucins, and cytokines.

131 c oxide, serum IgE, periostin, and blood and sputum eosinophils.

132 whether blood eosinophils are predictive of sputum eosinophils.

133 flammation in COPD should consider assessing sputum eosinophils.

134 r for COPD severity or exacerbations, or for sputum eosinophils.

135 of 100,000 individuals undergoing diagnostic sputum evaluation with Xpert for suspected pulmonary TB,

136 Sputum examination can be useful in diagnosing the cause

137 for desmosine measurement, and 381 provided sputum for baseline elastase activity measurements using

138 Detailed clinical data and sputum for culture were collected at baseline, 2 months,

139 Of these, sputum from 2608 (69.1%) met the quality criterion of <1

140 OSM protein levels were increased in induced sputum from asthmatic patients compared with that from c

141 Sputum from asthmatic patients with stable disease or ac

142 ing healthy control neutrophils with soluble sputum from patients with COPD.

143 ility of nanoparticles in fibrin gels and in sputum from patients with cystic fibrosis (CF).

144 Sputum from patients with tuberculosis contains subpopul

145 s may be a useful quality measure of induced sputum from young children with pneumonia.

146 We evaluated induced sputum Gram stain smears and cultures from hospitalized

147 umonia was considered pneumococcal if either sputum Gram stain, sputum culture, blood culture, or the

148 es were less than those observed between the sputum groups.

149 associated with neutrophilic asthma and with sputum IL-1beta protein levels, whereas eosinophilic ast

150 Aged asthmatic patients had higher sputum IL-6 (P < .01) and IL-8 (P = .01) levels.

151 In aged asthmatic patients increased sputum IL-6 and macrophage inflammatory protein 3alpha/C

152 Immunoprecipitated sputum immunoglobulins from patients with increased auto

153 The ability of sputum immunoglobulins to induce eosinophil degranulatio

154 Pseudomonas aeruginosa(Pa) was present in CF sputum in 11 patients, 4 had identical Pa strains in the

155 adiograph interpretation, utility of induced sputum in children, measurement of pathogen density, and

156 d concentrations of eosinophils in blood and sputum in chronic obstructive pulmonary disease (COPD) h

157 eruginosa transcript profiles in RNA from CF sputum indicated alginate production in vivo, and transc

158 Sputum, induced sputum, or bronchial specimens are all s

159 l characteristics, and sputum cytology after sputum induction were examined.

160 ies eosinophilic asthma without the need for sputum induction.

161 ing was reinitiated 2 hours after undergoing sputum induction; this death was categorized as "possibl

162 Sputum inflammatory measures decreased significantly in

163 Induced sputum is occasionally used to investigate lower respira

164 Induced sputum (IS) may provide diagnostic information about the

165 The incremental value of a repeated induced sputum (IS) sample, compared with a single IS or gastric

166 respiratory viruses and bacteria in induced sputum (IS) specimens from children hospitalized with se

167 0.60 +/- 0.01 nM (4.80 +/- 0.08 nmol kg(-1) sputum) is reached for both biomarker targets under the

168 A positive correlation was observed between sputum lactate and IL-1beta levels, and lactate content

169 technique removes residual DTT generated in sputum liquefaction and facilitates immunophenotyping of

170 When available, high-quality sputum may be useful for testing in hospitalized CAP pat

171 grew as free-floating biofilms in artificial sputum medium, mimicking sputum of CF lungs where P. aer

172 linical measures of disease severity and the sputum microbiome.

173 Sputum microscopy and culture are commonly used for diag

174 evaluated the diagnostic utility of induced sputum microscopy and culture in patients enrolled in th

175 Induced sputum microscopy and culture results were not associate

176 We assessed sputum microscopy with fluorescein diacetate (FDA, evalu

177 Total sputum mucins (but not mucin 5AC) were reduced at 24 hou

178 ion was assessed on the total RNA of induced sputum (n = 83), nasal brushings (n = 41), and endobronc

179 sing PTB but will be more cost-effective for sputum-negative patients and in settings with high preva

180 Sputum NET complexes were associated with the severity o

181 teria was reduced in patients with increased sputum NET complexes.

182 rved regardless of the method of quantifying sputum NETs.

183 Aged asthmatic patients had higher sputum neutrophil (30.5 x 10(4)/mL and 23.1%) and eosino

184 Inflammatory phenotypes were defined by sputum neutrophil and eosinophil cell proportions.

185 matic patients are associated with increased sputum neutrophil and eosinophil values and cytokine lev

186 ilia than cluster T1, with no differences in sputum neutrophil counts and exhaled nitric oxide and se

187 3 genes with the strongest relationships to sputum neutrophil counts were IL1R1 (standardized regres

188 Sputum neutrophil elastase activity is a biomarker of di

189 RATIONALE: Sputum neutrophil elastase and serum desmosine, which is

190 with decreased asthma control and increased sputum neutrophil numbers and IL-1beta, IL-6, and macrop

191 Multivariate regression confirmed that sputum neutrophil proportion was the strongest predictor

192 Sputum neutrophil proportions, but not eosinophil propor

193 ing gammaT treatment, focusing on changes in sputum neutrophilia, mucins, and cytokines.

194 e asthma and were positively associated with sputum neutrophilia.

195 n was restricted to neutrophilic asthmatics (sputum neutrophils >/= 76%), while eosinophilic asthmati

196 nophils (r=0.54; P<0.005) and inversely with sputum neutrophils (r=-0.46: P<0.05), but not with FEV1

197 emales using the OCP had significantly lower sputum %neutrophils than those not using the OCP (23.2 +

198 one and OCP use to be negative predictors of sputum %neutrophils, while C-reactive protein and IL-6 w

199 protein and IL-6 were positive predictors of sputum %neutrophils.

200 films in artificial sputum medium, mimicking sputum of CF lungs where P. aeruginosa is an important p

201 fically and significantly upregulated in the sputum of human patients with active tuberculosis.

202 Where studied, the majority of Mtb in the sputum of most untreated subjects with active TB have be

203 ion of rapidly growing mycobacteria from the sputum of patients with CF.

204 f nontuberculous mycobacteria (NTM) from the sputum of patients with cystic fibrosis (CF) is challeng

205 tuberculosis was quantified in pretreatment sputum of patients with pulmonary tuberculosis using FDA

206 otein levels were significantly increased in sputum of patients with severe asthma and were positivel

207 miR-142-3p was significantly upregulated in sputum of patients with severe asthma compared with that

208 thogen Staphylococcus aureus directly in the sputum of pediatric patients with cystic fibrosis (CF),

209 her high eosinophil concentrations in either sputum or blood are associated with a severe COPD phenot

210 ed that blood eosinophil counts, rather than sputum or tissue eosinophil counts, evolved as a pharmac

211 Sputum, induced sputum, or bronchial specimens are all suitable specimen

212 Ivacaftor caused marked reductions in sputum P. aeruginosa density and airway inflammation and

213 In the CF sputum, particle diffusion was spatially heterogeneous a

214 he severity of asthma or a specific cellular sputum pattern may be linked to evidence of innate immun

215 myalgia, cough, sore throat, runny nose and sputum), paucisymptomatic (1 symptom only), or asymptoma

216 ia" (nasopharyngeal/oropharyngeal or induced sputum PCR-positive without confirmed/suspected bacteria

217 an automated, integrated system for on-chip sputum processing and analysis.

218 es bacterial infection with increased cough, sputum production, and airflow obstruction.

219 There were significant differences in sputum proteomics and transcriptomics between the cluste

220 The clusters were compared based on sputum proteomics and transcriptomics data.

221 an visited on our hospital because of cough, sputum, pruritus and erythema.

222 Ivacaftor produced rapid decreases in sputum Pseudomonas aeruginosa density that began within

223 Extensive cytokine profiling of sputum revealed a TH2-dominated microenvironment (eotaxi

224 the XtracTB Assay was 5 genomic copies/ml of sputum rivaling that of culture.

225 rated that an Xpert test on 1 unconcentrated sputum sample (assuming equivalent results for unconcent

226 B LAM), and a molecular assay performed on a sputum sample (Xpert MTB/RIF; repeated if first result w

227 Two patients who were sputum sample negative had MABSC isolated in their gastr

228 by providing instruction on how to produce a sputum sample taken at any time of the day.

229 erculosis is dependent on the quality of the sputum sample tested.

230 One induced sputum sample was available for analysis from 3772 (89.1

231 stic performance data were calculated at the sputum-sample level, except where authors only reported

232 Sputum samples (n = 426) were tested with microscopic-ob

233 Most studies investigating relapse use sputum samples although tissue bacteria may play an impo

234 used to detect Mycobacterium tuberculosis in sputum samples and to obtain drug susceptibility informa

235 lded higher rates of detection for CIF in CF sputum samples compared with that detected in bacterial

236 Hphz can be directly measured in solubilized sputum samples diluted 20 times with the assay buffer.

237 2, and type 17 lymphocytes were measured in sputum samples from 48 participants with asthma.

238 We examined alterations in glycolysis in sputum samples from asthmatic patients and primary human

239 re we show that BPIFA1 levels are reduced in sputum samples from asthmatic patients and that BPIFA1 i

240 P. aeruginosa We were able to detect PQS in sputum samples from CF patients infected with P. aerugin

241 ected pulmonary tuberculosis, obtained three sputum samples from each participant, and compared the a

242 P. aeruginosa strains as well as RNA serial sputum samples from four P. aeruginosa-colonized subject

243 Comparisons were made between induced sputum samples from hospitalized children with radiograp

244 ing for rapidly growing NTM in all submitted sputum samples from patients with CF.

245 differential gene and protein expression in sputum samples from patients with severe asthma (SA) com

246 One hundred sixty-six out of 176 (94.3%) sputum samples from the Republic of Moldova yielded comp

247 ug resistance profiles directly from patient sputum samples has the potential to enable comprehensive

248 is much below the reported concentrations on sputum samples obtained from infected patients (up to 10

249 alyses to detect bacterial species in 945 CF sputum samples that had been previously analyzed by sele

250 r of observation and provided at least three sputum samples were classified by inflammatory phenotype

251 Sputum samples were collected for Xpert MTB/RIF testing

252 Sputum samples were collected from each inpatient and an

253 linical history, physical examination, and 3 sputum samples were obtained for direct fluorescent AFB

254 Expectorated sputum samples were sent for staining, mycobacterial cul

255 results for unconcentrated and concentrated sputum samples) is the most cost-effective strategy (99.

256 In a blind study of 24 clinical sputum samples, resistance mutations were detected in al

257 olving 8967 participants who provided 19 252 sputum samples.

258 phenotyping of major inflammatory cells from sputum samples.

259 0-fold genome-wide coverage) with sequential sputum sampling, we were able to detect transient geneti

260 24, 2016, we enrolled 2368 participants for sputum sampling.

261 okers using clinical parameters of cough and sputum scores, lung function, and chest high-resolution

262 gnostic test for tuberculosis, compared with sputum smear microscopy (the standard of care).

263 sts fail to equal culture's sensitivity with sputum smear microscopy negative specimens and therefore

264 ed at the point-of-care by the Ziehl-Neelsen sputum smear microscopy test.

265 e to their speed and sensitivity compared to sputum smear microscopy.

266 was present in 34% of samples, and 25% were sputum smear negative.

267 pert MTB/RIF had 100% and 81% sensitivity in sputum smear-positive and smear-negative groups, respect

268 chest in diagnosing pulmonary TB cases whose sputum smears are negative and making a correlation betw

269 Microscopic examination of acid-fast-stained sputum smears is the current standard of care in the Uni

270 These differences were sputum specific because no activation of NLRP3 or enrich

271 nt, and each participant gave at least three sputum specimen on 2 separate days.

272 ert Ultra diagnostic performance in the same sputum specimen was compared with culture tests and drug

273 of potential pathogens cultured from induced sputum specimens and quantity of oropharyngeal flora wer

274 study do not support the culture of induced sputum specimens as a diagnostic tool for pneumonia in y

275 The microbial composition of induced sputum specimens collected from adult patients screened

276 atings have never been evaluated for induced sputum specimens from children with suspected pneumonia.

277 th matched specimens, a higher proportion of sputum specimens had >/=1 pathogen detected compared wit

278 assess the cellular quality of expectorated sputum specimens to check that they originated from the

279 pplied multipathogen testing to high-quality sputum specimens to determine if more pathogens can be i

280 wards between March-June 2013 were enrolled; sputum specimens were collected and tested by GeneXpert,

281 Of 3772 induced sputum specimens, 2608 (69%) had <10 SECs per low-power

282 All participants provided two sputum specimens: one for the Xpert test and the other f

283 cationic protein (ECP) were also measured in sputum supernatant.

284 Lactate content was significantly higher in sputum supernatants from asthmatic patients, notably tho

285 ave decreased LXA4 concentrations in induced sputum supernatants in comparison with children with IA.

286 We investigated miRNA expression in sputum supernatants of 10 healthy subjects, 17 patients

287 gyrB open reading frames in their respective sputum TB isolates.

288 ement as at least a referral, chest X-ray or sputum test, 41% (111 of 274) SPs were correctly managed

289 rum-sensing regulation were less abundant in sputum than strains grown in the laboratory.

290 Sputum total bacterial concentrations also decreased, bu

291 ients were systematically investigated using sputum, urine and blood testing.

292 ections (245 versus 140) were more common in sputum versus NP/OP specimens, respectively, in both chi

293 Elastase activity in sputum was associated with the bronchiectasis severity i

294 trol for inhaled corticosteroid use, induced sputum was collected.

295 integrin beta3 mRNA accumulation in induced sputum was detected, to our knowledge, for the first tim

296 Induced sputum was obtained from nonsmoking patients with SA, sm

297 Induced sputum was obtained, and airway hyperresponsiveness to m

298 es and data on total mucin concentrations in sputum were also analyzed in an independent 94-participa

299 Total mucin concentrations in sputum were measured with the use of size-exclusion chro

300 uartile range [IQR], 47-386) bacteria/microL sputum, which was 5.1% (IQR, 2.4%-11%) the concentration