ライフサイエンスコーパス: ssRNA

1 ssRNA (single stranded) viral genomes are generally cons

2 ssRNA binding, but not ssDNA, induces higher-order oligo

3 ssRNA can interact with dendritic cells (DCs) through bi

4 ssRNA is channeled through its multisubunit ring-like co

5 Furthermore, HIV-1 ssRNA induced histone 4 acetylation at the TNFalpha prom

6 In the current study, HIV-1 ssRNA induced TNFalpha release in a dose-dependent manne

7 mulated with HIV-1 infectious virions, HIV-1 ssRNA, TLR 7 and 8 agonists, or IFN-alpha.

8 knockdown with relevant siRNA reduced HIV-1 ssRNA-mediated TNFalpha release, but silencing TLR7 had

9 entering the substrate from the extended 3' ssRNA terminus.

10 re from sequence, constructing equivalent 3D ssRNA models, transforming the 3D ssRNA models into ssDN

11 ivalent 3D ssRNA models, transforming the 3D ssRNA models into ssDNA 3D structures, and refining the

12 double-strand regions and extended 3' or 5' ssRNA overhangs in the adjacent ssRNA regions.

13 These results identify RIG-I as a ssRNA sensor and potential target of viral immune evasio

14 In the presence of a ssRNA target containing adjacent sequences complementary

15 Rhinovirus (RV), a ssRNA virus of the picornavirus family, is a major cause

16 ly arrayed coat protein (CP) protecting a (+)ssRNA.

17 ded 3' or 5' ssRNA overhangs in the adjacent ssRNA regions.

18 This ratio is conserved across all ssRNA/ssDNA viruses with highly basic peptide arms, and

19 suggesting that it should be similar for all ssRNA viruses with a comparable ratio of capsid size/gen

20 Further results on alternative ssRNA viral genomes are presented to illustrate more gen

21 the genome is indeed a primary factor among ssRNA viruses' evolutionary constraints, contributing al

22 An ssRNA analog induced TNF-alpha and IFN-gamma-induced pro

23 mplex from Thermotoga maritima can cleave an ssRNA target that is complementary to the CRISPR RNA.

24 n onto the surface were used to determine an ssRNA surface density of 4.0 x 10(12) molecules/cm2 and

25 and obtain different RT-PCR patterns for an ssRNA knot and circle of the same sequence.

26 al gene transfer might have occurred from an ssRNA virus to a dsRNA virus, which may provide new insi

27 HCV is an ssRNA virus, which suggests a role for Toll-like recepto

28 erties of MazF, a bacterial toxin that is an ssRNA- and ACA-specific endoribonuclease.

29 Qbeta, an ssRNA phage specific for the conjugative F-pilus, has a

30 graphic octamer and that both NSP566-188 and ssRNA bind to the grooves in the octamer, which are line

31 bstantial advantages of CircLigase-based and ssRNA-based capture for defining sequences and structure

32 Utilizing analogs of dsRNA (poly(I:C)) and ssRNA (ssRNA40), we demonstrated that an NLRP3-mediated

33 s into the evolutionary history of dsRNA and ssRNA viruses.

34 f single-stranded (ss) DNA geminiviruses and ssRNA viroids, respectively, but both pathogens can coun

35 or-1; and 3) significantly increased LPS and ssRNA-induced secretion of TNF-alpha and IL-6, as determ

36 ctivities that catalyze crRNA maturation and ssRNA degradation.

37 of NSP2 alone and in complexes with NSP5 and ssRNA at subnanometer resolution.

38 Here, we report measurements of ssDNA and ssRNA elasticity in the intermediate-force regime, corre

39 y, whereas it binds efficiently to ssDNA and ssRNA, about half as well to a DNA/RNA hybrid, and poorl

40 siRNAs and ssRNAs competitively inhibited human Dicer activity, sug

41 These data suggest that the siRNAs and ssRNAs interact predominantly with the PAZ domain of the

42 gene transfer between eukaryotic (+) and (-)ssRNA viruses.

43 el (HMM) framework which is used to annotate ssRNA viral genomes.

44 sors of viral infection as their ligands are ssRNA and dsDNA, respectively.

45 gonists consisting of nucleic acids, such as ssRNA or DNA with CpG motifs, activate the innate immune

46 , whereby endogenous ligand, Ro60-associated ssRNA, forges a nexus between TLR ligation and fibrosis

47 ddressed the hypothesis that Ro60-associated ssRNAs link macrophage activation to fibrosis via TLR en

48 cytokines in strict dependence on bacterial ssRNA and the host molecules MyD88 and UNC-93B.

49 In single-stranded RNA bacteriophages (ssRNA phages) a single copy of the maturation protein bi

50 We find that A3A binds ssRNA, but the RNA and DNA binding interfaces differ and

51 rovided a source of sequence-nonspecific but ssRNA-targeted in vitro ribonuclease activity that coelu

52 gonists, TLR7(rsq1) fails to be activated by ssRNA.

53 Activation of TLR by ssRNA after FcgammaR-mediated phagocytosis of immune com

54 The use of DNA-modified SiNPs to capture ssRNA for profiling has several advantages as compared t

55 forms of linear and covalently circularized ssRNA molecules.

56 mr effector complex has been found to cleave ssRNA in vitro.

57 binding above that achieved by a random-coil ssRNA.

58 We also show that binding of a complementary ssRNA target activates an ssDNA-specific nuclease activi

59 the Argonaute (Ago) family to complementary ssRNA targets: RNA-guided RNA interference.

60 By using a constructed ssRNA knot as a highly sensitive topological probe, we f

61 nism through which extracellularly delivered ssRNA contributes to CNS damage and determine an obligat

62 ly related to another IncP-plasmid-dependent ssRNA phage, PRR1.

63 on with TLR 7 and 8 ligands or HIV-1-derived ssRNA.

64 However, targeting any desired ssRNA remained elusive as two argonaute domains provided

65 nted proteins in the presence of any desired ssRNA target.

66 dRP that can synthesize dsRNA from different ssRNA templates using either a primer-dependent or prime

67 hogonal sets of crRNAs and possess different ssRNA cleavage specificities.

68 Statistical co-occurrence analyses of dsDNA, ssRNA and dsRNA viral markers of polyadenylation-selecte

69 nthesis and shifted the ratio of viral dsRNA/ssRNA in favor of dsRNA.

70 ing evidence for functionality of endogenous ssRNA species.

71 of the innate immune system sense endosomal ssRNA to detect infection by RNA viruses.

72 dependent on the expression of the endosomal ssRNA receptor TLR7.

73 ial for ssRNA cleavage, although it enhances ssRNA targeting for crRNAs encoded internally within the

74 amily Bunyaviridae), a group of enveloped (-)ssRNA viruses.

75 tation abolishes the unknotting activity for ssRNA, but not for ssDNA.

76 3a pre-crRNA processing is not essential for ssRNA cleavage, although it enhances ssRNA targeting for

77 the potential of the Phylo-HMM framework for ssRNA viral genomic annotation.

78 However, many current methods for ssRNA-seq suffer from the underrepresentation of both th

79 TLR7 is the mammalian receptor for ssRNA and some nucleotide-like small molecules.

80 prone mice deficient in TLR7, a receptor for ssRNA, failed to generate Abs to RNA-containing antigens

81 we propose a two-step assembly strategy for +ssRNA viruses: step I, acquisition of packaging specific

82 the structural and dynamic behavior of free ssRNAs at atomic resolution.

83 Furthermore, ssRNA-TNP uptake is dependent on macropinocytosis and cl

84 osaic virus (RCNMV) packages its two genomic ssRNAs via a specific capsid protein (CP) genomic RNA in

85 a preference of ssDNA approximately dsDNA > ssRNA, which is distinct from Rx1.

86 n these Antarctic RNA virus metagenomes had +ssRNA genomes most closely related to viruses in the ord

87 exosome-packaged HCV, cell-free HCV, or HCV ssRNA induced differentiation into MPhis with high M2 su

88 exosome-packaged HCV, cell-free HCV, or HCV ssRNA.

89 Further, we demonstrated that HCV ssRNA and other TLR7/8 ligands promote MPhi polarization

90 The HCV ssRNA-induced monocyte activation and differentiation in

91 macrophages, TLR8 binds and internalizes HIV ssRNA, leading to endosomal acidification, chromatin rem

92 , their individual roles in TLR-mediated HIV ssRNA recognition are unclear.

93 ch leads to the release of two copies of HIV ssRNA.

94 and promotes strand exchange with homologous ssRNA or ssDNA.

95 e cooperativity in the binding to homopurine ssRNAs indicates that the type of nucleic acid base dram

96 me shows a preference for the homopyrimidine ssRNAs.

97 However, ssRNA cleavage measured by qRT-PCR underestimated inacti

98 ing guide RNA to target and inhibit a human +ssRNA virus, hepatitis C virus, within eukaryotic cells.

99 ternal autoantibodies necessary for CHB, hY3 ssRNA, and affinity-purified anti-Ro60 antibody induces

100 These results identify ssRNA as a ligand for TLR7 and suggest that cells of the

101 with 5'-end phosphorylation vastly improves ssRNA activity both in vitro and in vivo.

102 viously unappreciated level of complexity in ssRNA, which we believe will also serve as an excellent

103 like protease domain that commonly exists in ssRNA viruses, including members of the families Potyvir

104 Fluorescence properties in ssRNA are defined by a small increase in average quantum

105 ity of viruses other than AaV, including (+) ssRNA viruses.

106 ce of a fully active RNAi suppressor induces ssRNA-specific ribonuclease activity, including that con

107 used by TLR2 activation was shown to inhibit ssRNA-induced IFN expression in dendritic cells.

108 Interestingly, ssRNA-TNP is more efficient to inhibit the expression of

109 t that the genomes of many viruses are large ssRNA molecules-often several thousand nucleotides long-

110 ading the dying cells with the TLR7/8 ligand ssRNA, whereas dying cells loaded with TLR3 ligand were

111 tes in response to uridine-rich (viral-like) ssRNA.

112 t homologous contacts can occur between long ssRNA and dsDNA in the absence of protein and that these

113 We propose that long ssRNA interacts paranemically with long dsDNA via period

114 Bacterial, yeast, and mammalian ssRNA encapsidated within HBcAg(183) all function as TLR

115 Many ssRNA/ssDNA viruses bind their genome by highly basic se

116 upport a key role for autophagy in mediating ssRNA virus detection and interferon-alpha secretion by

117 Of the three methods, ssRNA capture was most effective in defining sequences t

118 te) derived from this fluorescently modified ssRNA positively signals a binding event upon interactio

119 tive routes of delivery, chemically modified ssRNAs could represent a powerful RNAi platform.

120 To date, for most ssRNA virions, only the structures of the capsids and th

121 ng signal (PS) with capsid protein(s) (most +ssRNA viruses so far studied); step II, cocondensation o

122 , and they possess diverse genomes of mostly ssRNA and dsRNA and, recently, circular ssDNA.

123 ection method can be used to detect multiple ssRNA sequences at concentrations as low as 100 fM in 50

124 Exclusion of single or multiple ssRNA segments in the packaging reaction or their additi

125 d siRNA-loaded triangular DNA nanoparticles (ssRNA-TNP).

126 Similarly, natural ssRNA cannot activate murine TLR8, leading to the belief

127 tion, and also smaller than those of natural ssRNAs that are not under evolutionary pressure to have

128 The NeC3PO:ssRNA and NeC3PO:ssDNA complexes fold like closed footba

129 The NeC3PO:ssRNA structure represents the only catalytic form C3PO

130 West Nile virus (WNV) is a neurotropic ssRNA flavivirus that can cause encephalitis, meningitis

131 Applying the method to new ssRNA-seq data from whole-root and cell-type-specific Ar

132 oat protein of a plant virus and a noncoding ssRNA molecule, are highly immunogenic in mice.

133 vant consists of a 547-nt uncapped noncoding ssRNA containing polyU repeats that is stabilized by a c

134 Both macrophage transfection with noncoding ssRNA that bind Ro60 and an IC generated by incubation o

135 with higher affinity than to other nonviral ssRNA sequences.

136 ict the average MLD values of large nonviral ssRNAs scale as N(0.67+/-0.01), where N is the number of

137 ed by single-stranded DNAs (ssDNAs), but not ssRNA or dsDNA.

138 Here we report a novel ssRNA-seq method that does not involve second-strand cDN

139 Furthermore, the NP-ssRNA structures presented here, combined with hydrogen-

140 (REMD) simulations to characterize the 12 nt ssRNA tail derived from the prequeuosine riboswitch.

141 ponses triggered by the synthetic analogs of ssRNA viruses (polyuridine) and dsRNA viruses (polyinosi

142 the factors that drive efficient assembly of ssRNA viruses in vivo.

143 utionary events for these two main clades of ssRNA phages.

144 otentially leading to incomplete cleavage of ssRNA and the release of short (3-5) nucleotide products

145 te site-specific endonucleolytic cleavage of ssRNA targets, similar to PAM-mediated stimulation of Ca

146 effect also depends on the concentration of ssRNA and is abolished by overstretching of the dsDNA.

147 ed with the generation of multiple copies of ssRNA via in situ surface transcription by RNA polymeras

148 ding interfaces differ and no deamination of ssRNA is detected.

149 agnetic tweezers, we measured the effects of ssRNA on force extension curves for dsDNA.

150 btain a deeper insight into the evolution of ssRNA phages, more phages specializing for various conju

151 curately capture the inherent flexibility of ssRNA loops, accurate base stacking energetics, and puri

152 between the mutually exclusive functions of ssRNA binding and dNTP hydrolysis depending on dNTP pool

153 TLR7 recognizes the genome of ssRNA viruses such as Coxsackievirus B.

154 order in the packaged genomes of a number of ssRNA viruses.

155 we measure the persistence lengths (l(p)) of ssRNA.

156 adjacent 5-fold vertex, the entry pathway of ssRNA segments.

157 We observe that the presence of ssRNA impedes the extension of dsDNA, specifically at lo

158 This structure, involving the recognition of ssRNA via a stem-loop conformation, together with our tw

159 ce, which both have defective recognition of ssRNA, and found increased viremia and susceptibility to

160 repeat proteins can target a limited set of ssRNA sequences, there are no general methods for target

161 Much of our understanding of ssRNA conformational behavior is limited to structures i

162 and Vienna computations on large numbers of ssRNAs of various lengths (1000-10 000 nt) and sequences

163 isplayed robust endoribonuclease activity on ssRNA with a preference for cleavage after purine-pyrimi

164 s B virus, and simian virus 40 assembling on ssRNA and dsDNA substrates.

165 en the RNA and DNA species, and dependent on ssRNA lengths (>/=1 kb).

166 impact of specific chemical modifications on ssRNA activity implies an Ago-mediated mechanism but the

167 l palm subdomain architecture of poliovirus (ssRNA+) RdRp could accommodate the identified sequence p

168 esium, the total site size of the polymerase-ssRNA complex is 26 +/- 2 nucleotides.

169 o repeat and expanded the scope of potential ssRNA targets.

170 y of a biocompatible gold nanorod, GNR-5'PPP-ssRNA nanoplex, as an antiviral strategy against type A

171 ctivity that was TBSV sequence-preferential, ssRNA-specific, divalent cation-dependent, and insensiti

172 TLRs irrespective of whether they recognize ssRNA, dsRNA or hypomethylated DNA.

173 rent study, the role of TLR7 that recognizes ssRNA was examined.

174 on the genome packaging of a representative ssRNA virus, the bacteriophage MS2, via a series of biom

175 study, we assessed the role of uridine-rich ssRNA derived from the HIV-1 long terminal repeat (ssRNA

176 parallel strand-specific sequencing of RNA (ssRNA-seq) has emerged as a powerful tool for profiling

177 ncing results in enhanced single-strand RNA (ssRNA) replication of RSVand Sendai virus, due to decrea

178 modifications to optimize single-strand RNA (ssRNA)-mediated mRNA knockdown.

179 ivated by viral genomic single-stranded RNA (ssRNA) bearing 5'-phosphates.

180 HCV double-stranded to single-stranded RNA (ssRNA) correlated positively with ISG induction.

181 a mosquito-transmitted single-stranded RNA (ssRNA) flavivirus, causes human disease of variable seve

182 l structures containing single-stranded RNA (ssRNA) free of strong base pairing interactions can be c

183 RVFV has a trisegmented single-stranded RNA (ssRNA) genome.

184 n assay between SLA and single-stranded RNA (ssRNA) indicate that SLA competes with ssRNA for the sam

185 t the binding of p33 to single-stranded RNA (ssRNA) is stronger than binding to double-stranded RNA (

186 hages, particularly the single-stranded RNA (ssRNA) leviviruses, have a previously unappreciated capa

187 Single-stranded RNA (ssRNA) molecules of nonviral origin also induce TLR7-dep

188 ence pathways use small single-stranded RNA (ssRNA) molecules that guide proteins of the Argonaute (A

189 arious short 20-24 base single-stranded RNA (ssRNA) oligonucleotides from a target solution.

190 Various unmodified single-stranded RNA (ssRNA) oligonucleotides were ligated onto identical 5'-p

191 Poly(I:C), but not single-stranded RNA (ssRNA) or a standard DC maturation cocktail, elicited ty

192 y of the positive-sense single-stranded RNA (ssRNA) Orsay virus (OV) as a natural pathogen of the nem

193 d sequentially as three single-stranded RNA (ssRNA) segments into an icosahedral procapsid which serv

194 sociation constant to a single-stranded RNA (ssRNA) sequence adjacent to the branch site and can bloc

195 are the active sites of single-stranded RNA (ssRNA) synthesis; (v) at late times postinfection, only

196 s with high affinity to single-stranded RNA (ssRNA) targets matching the Cas9-associated guide RNA se

197 stranded RNA (dsRNA) or single-stranded RNA (ssRNA) templates.

198 model of SmAP bound to single-stranded RNA (ssRNA) that explains Sm binding-site specificity.

199 ayer particles, nascent single-stranded RNA (ssRNA) transcripts (termed in vitro ssRNA) were found to

200 ay have occurred from a single-stranded RNA (ssRNA) virus (hypovirus) to a dsRNA virus, SsMBV1.

201 which resembles that of single-stranded RNA (ssRNA) viruses but differs from the well-established mec

202 Single-stranded RNA (ssRNA) viruses form a major class that includes importan

203 DC responses to certain single-stranded RNA (ssRNA) viruses occur only after live viral infection.

204 While most T=3 single-stranded RNA (ssRNA) viruses package in vivo about 3,000 nucleotides (

205 nto a preformed capsid, single-stranded RNA (ssRNA) viruses, such as bacteriophage MS2, co-assemble t

206 Single-stranded RNA (ssRNA) viruses, which include major human pathogens, pac

207 e-stranded DNA (dsDNA), single-stranded RNA (ssRNA), and ssDNA/reverse-transcribing viruses could be

208 and uridine-rich viral single-stranded RNA (ssRNA), including influenza virus ssRNA.

209 o2) and Cryptosporidium single-stranded RNA (ssRNA), we induced specific slicing in Cryptosporidium R

210 were used to map three single-stranded RNA (ssRNA)-binding regions within the protein.

211 nt from the upper stem-single-stranded RNA (ssRNA, terminal loop) junction or ~11 nt from the lower

212 e with positive-sense, single-stranded RNA (+ssRNA) genomes, are abundant in tropical and temperate c

213 ibiting positive-sense single-stranded RNA (+ssRNA) viral infection, especially since RNA is not know

214 in the positive-sense, single-stranded RNA [ssRNA(+)] arteriviruses.

215 -borne, negative-sense, single-stranded RNA [ssRNA(-)] nairovirus that produces fever, prostration, a

216 leoside triphosphatase, single-stranded RNA [ssRNA] binding and helix-destabilizing activities) and N

217 enic positive-sense, single-stranded RNA [(+)ssRNA] virus families which carry a macro domain: Corona

218 oducts (siRNA) and short single-strand RNAs (ssRNA).

219 Single-stranded RNAs (ssRNAs) are ubiquitous RNA elements that serve diverse f

220 s upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a posit

221 equences, and not with single-stranded RNAs (ssRNAs) or double-stranded DNA.

222 lpha and beta by short single-stranded RNAs (ssRNAs) transcribed with T3, T7 and Sp6 RNA polymerases.

223 hese highly structured single stranded RNAs (ssRNAs) with emphasis on their presence and function in

224 60 and an IC generated by incubation of Ro60-ssRNA with an IgG fraction from a CHB mother or affinity

225 dent single-stranded RNA bacteriophages (RPD ssRNA phages), C-1 and Hagl1.

226 escent reporter strain of the negative-sense ssRNA vesicular stomatitis virus (VSV), we show that mic

227 Its positive-sense ssRNA genome of 3,569 bases is enclosed in a capsid with

228 , we have used four strand-specific RNA-seq (ssRNA-seq) datasets collected under two experimental con

229 e strand-specific paired-end RNA sequencing (ssRNA-seq) data from 376 cancer samples covering nine ti

230 scription is strand-specific RNA sequencing (ssRNA-seq).

231 Subsequently, the medium to larger size ssRNAs are recruited until the complete genome is packag

232 osterna elaeasa virus (EeV), insect-specific ssRNA+ viruses, which revise a capsid-based classificati

233 of genome-capsid interactions in a spherical ssRNA virus provides insight into genome delivery via th

234 co mosaic virus (STMV) is a small, spherical ssRNA virus common in a natural wild plant, Nicotiana gl

235 between the viral genome types dsDNA, ssDNA, ssRNA positive strand, ssRNA negative strand and retroid

236 iral sequences by genome type (dsDNA, ssDNA, ssRNA positive strand, ssRNA negative strand, retroid) u

237 loop) junction or ~11 nt from the lower stem-ssRNA junction to determine the cleavage site.

238 distances from both the lower and upper stem-ssRNA junctions to determine the cleavage site in human

239 atory syncytial virus (RSV), negative strand ssRNA virus, depends upon the ability to recognize speci

240 , some having dsRNA and some positive-strand ssRNA genomes.

241 termediate between dsRNA and positive-strand ssRNA viruses, as well as between encapsidated and capsi

242 e types dsDNA, ssDNA, ssRNA positive strand, ssRNA negative strand and retroid and amino acid prefere

243 e type (dsDNA, ssDNA, ssRNA positive strand, ssRNA negative strand, retroid) using amino acid distrib

244 avage sites were not observed which suggests ssRNA may operate through a mechanism beyond conventiona

245 Cs by polyuridylic acid (polyU), a synthetic ssRNA analog, generates a strong specific cytotoxic resp

246 , there are no general methods for targeting ssRNA with designed proteins.

247 nded DNA with three-fold lower affinity than ssRNA of the same length and sequence, while binding to

248 Prior studies have shown that ssRNA binds to NPH I, but it does not activate ATPase ac

249 We report in this study that ssRNA causes neurodegeneration and neuroinflammation dep

250 The ssRNA-modified SiNPs are collected from the target solut

251 The ssRNA-TNP is formed by the complementary association of

252 However, the ssRNA contained within HBcAg(183) does function as a TLR

253 oncentrated prior to detection; and (iv) the ssRNA-modified SiNPs give an enhanced SPRI signal upon h

254 as well as to verify the bioactivity of the ssRNA microarray in terms of (i) the hybridization adsor

255 the SiNPs enhance the diffusion rate of the ssRNA to the surface; (iii) the SiNPs can be collected,

256 sDNA that is complementary to one end of the ssRNA transcript.

257 This binding site is similar to the ssRNA-binding site of the sterile alpha motif domain of

258 nerate a series of deletion mutants with the ssRNA-binding domains of NS2 removed, singly and in diff

259 The Kd values for the ssRNAs ranged from 3- to 40-fold weaker than the Kd for

260 sition of the 3'-terminal nucleotides of the ssRNAs exhibited the greatest effect on Dicer binding.

261 e dsDNA with an affinity comparable with the ssRNAs affinity, indicating that the binding site has an

262 This ssRNA-TNP has a simpler structure, better stability, and

263 As a result, the three ssRNA-binding domains of BTV nonstructural protein NS2 h

264 addition, YdbC binds with lower affinity to ssRNA, making it a versatile nucleic acid-binding domain

265 tely abrogates the ability of NS2 to bind to ssRNA.

266 Because TLR7(rsq1) binds to ssRNA, our studies imply that the N-terminal portion of

267 e annotation and applies an HMM framework to ssRNA viral annotation.

268 ously identified TUTase that adds many Us to ssRNA and which we find is neither a stable editosome co

269 e affinity of each truncated protein towards ssRNA was then assayed by electrophoretic mobility shift

270 sequence conservation between RdRps of true ssRNA+ and dsRNA viruses and form a minor, deeply separa

271 We used unique characteristics of two ssRNA viruses, dengue virus and influenza virus, to deli

272 the host sex pilus and mechanisms underlying ssRNA-capsid co-assembly, and inspires speculation about

273 To identify cis-NATs using ssRNA-seq, we developed a new computational method based

274 After recognition of a viral ssRNA genome, Nod2 used the adaptor protein MAVS to acti

275 matory proteins upon bacterial LPS and viral ssRNA stimulation was higher in CM and NCM of the Ob gro

276 g antiviral effects when stimulated by viral ssRNA.

277 evidence that synonymous mutations in viral ssRNA genomes are not strictly neutral.

278 TLR8 recognize specific intracellular viral ssRNA sequences, but in human alveolar macrophages, thei

279 yD88-mediated events, as do sensing of viral ssRNA and the drug imiquimod.

280 ly synthetic TLR7/8 ligand, a mimic of viral ssRNA, induced IL-23 production by LP CD1c+ DCs, and thi

281 n dNTP pool levels and the presence of viral ssRNA.

282 TLR7 and TLR8 and mouse TLR7 recognize viral ssRNA motifs and induce antiviral immunity.

283 e receptors (TLR) 7 and 9, which sense viral ssRNA and CpG DNA, respectively.

284 length, we predict the R(g) values of viral ssRNAs are smaller than those of nonviral sequences.

285 The MLD values of viral ssRNAs that package into capsids of fixed size are shown

286 encoded non-structural protein NSP2 to viral ssRNAs results in the remodeling of RNA, which is conduc

287 anded RNA (ssRNA), including influenza virus ssRNA.

288 Enzymatic studies demonstrated that in vitro ssRNA and in vivo 6 hr large RNA samples contain uncappe

289 to innate immune signaling by both in vitro ssRNA and in vivo 6 hr large RNAs.

290 ded RNA (ssRNA) transcripts (termed in vitro ssRNA) were found to be potent IFN inducers.

291 of newly generated nucleotide overhangs when ssRNA is targeted by a designed complementary guide sequ

292 l behavior is limited to structures in which ssRNA directly engages in tertiary interactions or is re

293 inding assays showed that NSP5 competes with ssRNA binding, indicating that one of the functions of N

294 RNA (ssRNA) indicate that SLA competes with ssRNA for the same binding site on the NS5 polymerase.

295 ing domain of Lassa virus NP in complex with ssRNA.

296 n also pair and promote strand exchange with ssRNA.

297 Competition experiments with ssRNA revealed that p33 binds to a TBSV-derived sequence

298 Supernatants generated from macrophages with ssRNA in the presence of IRS661 or chloroquine did not c

299 n levels by transfecting live parasites with ssRNA-hAgo2 complexes.

300 generated from macrophages transfected with ssRNA or incubated with the IC.