ライフサイエンスコーパス: stable expression

1 egative mutant), and derived cell lines with stable expression.

2 Nicotiana species during both transient and stable expression.

3 some technology provides one solution to the stable expression and maintenance of multiple transgenes

4 th transmembrane domain is required for Ser5 stable expression and plasma membrane localization.

5 ra transmembrane domain is required for Ser5 stable expression and plasma membrane localization.

6 The stable expression and safe properties of this vector cou

7 ntial for its association with beta-catenin, stable expression at the cell surface, and consequently,

8 ne protein that requires O-glycosylation for stable expression at the cell surface.

9 nt of cell lines with predictable, long-term stable expression capacity are based on single-copy inte

10 orphine-6-glucuronide (M6G) were observed in stable expression cells, HEK293-hOATP2B1 than HEK293-MOC

11 duced apoptosis in recipient cells; however, stable expression could be established in cells that exp

12 of individual alleles and was controlled by stable expression differences of the Th1 cell lineage-sp

13 tion with the oncogenic process resulting in stable expression during disease progression when the pr

14 Rho showed stable expression even in the absence of endogenous Rh1

15 in HD-AduPA arteries for > or =56 days, with stable expression from 14 to 56 days.

16 -14-kb Pu.1 or +37-kb Cebpa enhancers after stable expression in 32Dcl3 cells.

17 DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and

18 que cellular gene, CEM15, whose transient or stable expression in cells that do not normally express

19 Upon stable expression in cultured tobacco (Nicotiana tabacum

20 -induced apoptosis in leukemic cells and its stable expression in epithelial tumor cells sensitized t

21 ntly overexpressed in many solid tumors, and stable expression in HeLa cells confers resistance to do

22 ed human factor VIII levels by transient and stable expression in multiple cell lines.

23 Stable expression in myoblasts of a CDO deletion mutant

24 the ALK(Delta4-11) variant were shown after stable expression in NIH3T3 cells.

25 ) was compared to wild-type rat alpha1 after stable expression in opossum kidney cells.

26 Here we report successful stable expression in primates of siRNAs directed to chem

27 Gbeta5 is sufficient for its stable expression in rods even in the absence of R9AP.

28 ng in vitro culture after six generations of stable expression in the multi-copy subline, T3#30, but

29 Stable expression in the orrm6 mutants of a nucleus-enco

30 DNA synthesis of human glioma cells on both stable expression in tumor-derived cell lines as well as

31 ct of seven PIK3R1 mutations was examined by stable expression in U2OS cells, followed by coimmunopre

32 Fe65 knockdown decreased, whereas its stable expression increased the transcriptional activity

33 Proteins with housekeeping functions and stable expression level such actin, tubulin, and glycera

34 of the 9406 genes analyzed showed relatively stable expression levels across different donors (for an

35 Stable expression of 14-3-3epsilon in HT-29 significantl

36 Stable expression of a BAF60a truncation mutant, BAF60a4

37 Stable expression of a beta(1) integrin mutated at this

38 Stable expression of a DEGA/AMIGO-2 antisense construct

39 Conversely, stable expression of a dominant negative ATF2 (dnATF2) q

40 We report the inducible, stable expression of a dominant negative form of mitocho

41 ty was down-regulated in resistant cells via stable expression of a dominant negative RNase L mutant,

42 Similarly, stable expression of a dominant negative TRAIL receptor

43 Importantly, stable expression of a dominant negative, oncogenic PML-

44 toplasmic sequestration of beta-catenin upon stable expression of a dominant-negative E-cadherin fusi

45 Stable expression of a dominant-negative mutant ERK elim

46 activation of endogenous Smad2 and Smad3 by stable expression of a mutant form of the TGF-beta type

47 Consistent with this notion, stable expression of a nonphosphorylatable CREB or a con

48 n, leading to their mutual stabilization and stable expression of a nuclear localization-deficient VD

49 Stable expression of a periostin transgene in tumorigeni

50 human pancreatic tumor cells was reduced by stable expression of a plasmid encoding small interferin

51 Stable expression of a single engineered betaTrCP demons

52 human prostate cancer cell line through the stable expression of a small interfering RNA (siRNA).

53 ized HC11 mouse mammary epithelial cells: 1) stable expression of a specific Jak2 antisense construct

54 transcription factor Helios by Tregs ensures stable expression of a suppressive and anergic phenotype

55 The stable expression of a Walker A/B mutant in THP-1 monocy

56 Furthermore, transient or stable expression of A56/K2 interfered with virus entry

57 Our data indicate that stable expression of activated KrasG12D confers on cells

58 indeed a transforming oncogene and show that stable expression of AEG-1 in normal immortal cloned rat

59 DNA in human and mouse macrophages, whereas stable expression of AIM2 in the non-responsive human em

60 tion of the rAAV-CAG-hAGA vector resulted in stable expression of alpha-gal A in organs of the Fabry

61 pecificity of FSHD pathology, which requires stable expression of an alternative full-length mRNA spl

62 ndogenous Drg1 expression in Hct116 cells by stable expression of an antisense (AS) Drg1 cDNA increas

63 tically engineered the P. putida KT2440 with stable expression of an arsM-gfp fusion gene (GE P. puti

64 studied this by generating HEK293 cells with stable expression of an isoform of the amylin receptor f

65 Furthermore stable expression of an isolated CARD of ASC in THP-1 ce

66 een these two features was apparent, because stable expression of Ang2 by U87MG cells or treatment of

67 The present study evaluated the potential of stable expression of angiostatin and endostatin before t

68 Stable expression of aspartic acid phosphomimetic (S490D

69 Stable expression of ATF2 in human breast carcinoma BT47

70 Stable expression of AtKRS in Zea mays causes translatio

71 sequent transformation events leading to the stable expression of bacterial DNA was unexplored.

72 Stable expression of Bcl-2 or Mcl-1 lowered ER Ca(2+) co

73 actions; and by modifying the evolutionarily stable expression of behavioural traits that are themsel

74 by chromatin immunoprecipitation assay upon stable expression of BEN.

75 It is based on the stable expression of BirA in the cytoplasm of maize plan

76 n miR-92b and Mef2 efficiently maintains the stable expression of both components that is required fo

77 The stable expression of botulinum toxin E to prevent exocyt

78 is Shq1p, an essential protein necessary for stable expression of box H/ACA snoRNAs.

79 In the current study, stable expression of BRCA1a/p110 resulted in inhibition

80 Furthermore, stable expression of Brd4-CTD enhanced the frequency of

81 In this work, the effect of the stable expression of Bs2 gene on citrus canker resistanc

82 Stable expression of C/EBPepsilonT75A in interleukin 3 (

83 Stable expression of C20zeta-EGFP in Madin-Darby canine

84 Additionally, stable expression of CaMKIIdeltaB promotes distinct morp

85 Consistent with these results, stable expression of CARD-8 in U937 or THP-1 cells sensi

86 Hence, stable expression of CatSper1 protein requires CatSper2

87 Notably, stable expression of CCL22 abrogated the antitumor effec

88 Stable expression of CD16 L66H in NK-92 cells recapitula

89 NA-methylation marks from Cd4, which allowed stable expression of Cd4 in helper T cells.

90 2006) we found that pDCs exhibit strong and stable expression of CD70, a tumor necrosis factor famil

91 Stable expression of cDNAs in HepG2 cells showed that th

92 The stable expression of cdNIPP1 disrupted the interaction o

93 Stable expression of cdNIPP1 increased CDK9 phosphorylat

94 Stable expression of CoAA, RBM4, or their variants preve

95 in PC-3 cells, which could be rescued by the stable expression of constitutively active ILK.

96 Stable expression of core protein induced frequent chrom

97 Stable expression of CRABP-II in mammary carcinoma SC115

98 creased in Chinese hamster ovary cells after stable expression of CYP3A4 in combination with P450 red

99 Stable expression of dominant negative (DN) Nod1 in colo

100 terfering RNA-mediated knockdown of STAT6 or stable expression of dominant-negative STAT6 decreased C

101 transposon mediated gene transfer to obtain stable expression of Doxycycline (Dox) regulated transge

102 Previous studies showed that the stable expression of DrrB in the membrane depends on sim

103 Stable expression of DUX4, however, can only occur in th

104 howed that in human MCF10A epithelial cells, stable expression of E4-ORF1 proteins encoded by represe

105 Stable expression of EID-2 in the TGF-beta1-responsive c

106 metastatic MDA-MB-231 breast cancer cells by stable expression of either short hairpin RNA-Runx2 or a

107 onica) line showing neuronal specificity and stable expression of enhanced green fluorescent protein

108 We established a method for stable expression of epitope-tagged TER and TER variants

109 Stable expression of ERG and Tmprss2:ERG variants promot

110 Like HA-Ets-2 and V12-Ras, stable expression of ESX induced EGF-independent prolife

111 ABC encode membrane proteins involved in the stable expression of esxA, esxB, and esaC, genes specify

112 Stable expression of Evi1 in non-transformed intestinal

113 y sequestered wt-p53 in neuroblastomas after stable expression of Ews/Fli-1.

114 ither DDX5 wild type (WT) or SNP cDNA, or 2) stable expression of exogenous DDX5 WT and SNP in HSC li

115 Furthermore, stable expression of exogenous PDRG was found to decreas

116 Stable expression of F-box-mutated FL ZTL eliminates the

117 Stable expression of feline immunodeficiency virus (FIV)

118 Following the stable expression of Flag:YB-1 and Flag:YB-1 (Ala102) in

119 hly expressed in iTregs and required for the stable expression of Foxp3 in iTregs in vitro and in viv

120 Stable expression of Foxp3 in regulatory T cells (Tregs)

121 As stable expression of Foxp3 is important for the ability

122 ted a large number of iTregs with relatively stable expression of Foxp3, which were able to prevent C

123 s not sufficient for imprinting T cells with stable expression of Foxp3.

124 TGF-beta stimulation, but failed to maintain stable expression of Foxp3.

125 rescue to light-induced cell death, whereas stable expression of functional CYP2C9-GFP fusion protei

126 Ric-8b in olfactory sensory neurons prevents stable expression of Galphaolf.

127 eatly reduced levels of REV1 mRNA due to the stable expression of gene-specific ribozymes, and compar

128 signaling pathways were further confirmed by stable expression of gene-specific shRNAs in hCSCs.

129 SSAT1 transient transfection did not affect stable expression of GFP, and stably expressed SSAT1 did

130 Stable expression of Gli2 in MDA-MB-231 cells resulted i

131 Stable expression of GMFG-S2E remarkably enhanced stimul

132 We used transient and stable expression of green fluorescent protein (GFP) GK

133 rcane plants, the ShCesA7 promoter conferred stable expression of green fluorescent protein preferent

134 Stable expression of GRPr was maintained after rapamycin

135 Stable expression of GTG1(pro):GTG1-GFP (for green fluor

136 In addition, stable expression of hemagglutinin-tagged GRA15II in THP

137 y typically requires high-level, genetically stable expression of heterologous genes and pathways.

138 Furthermore, stable expression of hsp16 prior to HIV-1 infection inhi

139 Stable expression of hTERT in OCa cells decreases their

140 Stable expression of human groups IIA and X secreted pho

141 We used zinc finger nucleases to induce stable expression of human imaging reporter genes into t

142 Stable expression of human PS1 restores these deficienci

143 fibroblasts into mouse skin resulted in the stable expression of human type VII collagen at the mous

144 Stable expression of human wild-type alphasyn at physiol

145 In contrast, stable expression of IFN-beta following adeno-associated

146 Stable expression of IKBSR, a known repressor of NF-kapp

147 Stable expression of ILK-KD in HER2/neu-transformed cell

148 Stable expression of integrin alpha5/beta1 in colon canc

149 Stable expression of intestine-specific molecular marker

150 IFNgamma induced a faster, higher, and more stable expression of IRF-1 than IFNgamma alone.

151 Here, we show that stable expression of IRF-4 in a human lymphoid cell line

152 ) was decreased in EML-TA cells; strikingly, stable expression of IRF3 restored the capacity of EML-T

153 Stable expression of IRS-1 confers to BT-20 cells the ab

154 Transient and stable expression of JAK1 and/or JAK3 mutants showed tha

155 We demonstrate that stable expression of K13, but not other FLIPs, in a vari

156 l motility by approximately 50%, and induces stable expression of key mesenchymal markers, including

157 y cells enhanced transferrin uptake, whereas stable expression of kinase-dead PIPKIgamma661 had an in

158 of human breast cancer, we first established stable expression of KLF8 in an immortalized normal huma

159 Moreover, stable expression of LePHGPx in tobacco conferred protec

160 Stable expression of LMP2A increased the protein level a

161 g of dendritic spines is associated with the stable expression of long-term potentiation (LTP), the r

162 Stable expression of long-term synaptic plasticity is cr

163 etroviral gene transfer system, we find that stable expression of LRP6 in human fibrosarcoma HT1080 c

164 Previously, we have shown that stable expression of LT protein results in upregulation

165 ard versus the reverse direction, leading to stable expression of Ly49 receptors in mature NK cells.

166 ted in cells of neuronal morphology that had stable expression of markers of neuronal differentiation

167 genase-2, were significantly up-regulated by stable expression of MDA-7.

168 lished cell model systems to examine whether stable expression of MEKK3 could lead to increased NFkap

169 Stable expression of microRNA 200b in As-transformed p53

170 In functional experiments, stable expression of miR-1258 in BMBC cells inhibited he

171 Here, we report that stable expression of miR-155 in 4T1 breast tumor cells r

172 Stable expression of moderate levels of p53 in Saos-2 ce

173 epatocyte spheroids in rocked culture showed stable expression of more than 80% of 242 liver-related

174 On the other hand, stable expression of Mrad9, the mouse homolog, in DU145/

175 ate the critical and complex role of CL5 for stable expression of MRP1 at the plasma membrane and mor

176 brane helix 9 (TM9) to TM10 are critical for stable expression of MRP1 at the plasma membrane.

177 Stable expression of MUC1 with the CQC --> AQA mutations

178 Silencing of MUC4 by transient or stable expression of MUC4-targeted short-interfering RNA

179 y plasmids, allowing efficient transient and stable expression of multiple autofluorescently tagged p

180 nal or glial markers, but instead showed the stable expression of multiple endothelial markers and th

181 ed for fluorescent tagging and transient and stable expression of multiple proteins in plant cells fr

182 was induced by tunicamyin administration or stable expression of mutant (L188Q) SFTPC in type II AEC

183 s 3D-F, Kan and colleagues demonstrated that stable expression of mutant GNAO1(R243D) conferred a sig

184 Stable expression of mutant kidney band 3 in both nonpol

185 Stable expression of mutant PIK3CB variants conferred re

186 In this model, stable expression of mutant, but not wild-type, human SO

187 by treatment with caspase inhibitors and by stable expression of mutant, noncleavable inhibitor of c

188 onversion to a mature cardiac phenotype with stable expression of myocytic markers (25%) in the absen

189 evels of Icmt are inhibited by methotrexate, stable expression of myristoylated H-Ras, which does not

190 Stable expression of NBCn1-E in human embryonic kidney c

191 Finally, stable expression of Nef in a retroviral vector, pBabe-p

192 Stable expression of NHERF-1 in the OKH parent (OKH-N1)

193 nome delivery by adeno-associated virus, and stable expression of NTCP in a ccHBV producing cell line

194 Stable expression of Numb isoforms that differ in the PT

195 Stable expression of OAPT2B1 in HeLa R1-11 cells resulte

196 Stable expression of ORRM6 rescues the orrm6 editing def

197 We show that both transient and stable expression of PDGF-C results in the development o

198 Moreover, stable expression of PDHK4 increased localization of act

199 h PELP1/MNAR expression is down-regulated by stable expression of PELP1/MNAR-specific shRNA.

200 Notably, stable expression of phospho-null mutant TRAF2 in cancer

201 -p53-SIVA1 interaction; however, this showed stable expression of phospho-p53, which increased signif

202 Stable expression of phosphomimetic Tyr-153 RhoGDI2 in m

203 Moreover, the stable expression of physiological levels of HIV-1 Tat d

204 Stable expression of Pip4k2beta in breast cancer cell li

205 Stable expression of PR-B in PR-low/ER+ MCF7 cells incre

206 We found that stable expression of PRL-3 confers cytokine independence

207 issue- and cell-specific expression based on stable expression of promoter-reporter gene constructs s

208 enic potential of PSF-TFE3 became evident by stable expression of PSF-TFE3 in NIH-3T3 mouse fibroblas

209 Similarly, the stable expression of pyrin in PMA-stimulated THP-1-deriv

210 uppression of dATP and dGTP levels caused by stable expression of R2-targeted short interference RNA.

211 Here we demonstrate that stable expression of Ras effector-loop mutants that acti

212 Furthermore, stable expression of RAX(S18A) results in a dominant-neg

213 o capacity was constructed for the rapid and stable expression of recombinant proteins in different p

214 Conversely, stable expression of RelB in androgen-responsive LNCaP t

215 ndent cell transformation assay (soft agar), stable expression of RSK2 in JB6 cells significantly enh

216 Knockdown of Brk by stable expression of short hairpin RNA (shRNA) in T47D b

217 in which IL-32 expression was silenced using stable expression of short hairpin RNA (shRNA).

218 RNA (siRNA) duplexes or lentivirus-mediated stable expression of short hairpin RNA inhibited the act

219 hich BPAG1-e expression had been silenced by stable expression of short hairpin RNA to target DST.

220 RNA silencing was achieved in plants through stable expression of short hairpin RNA, which was driven

221 resolved these questions in C4-2 cells using stable expression of short hairpin RNAs to knock down ex

222 t with either a gamma-secretase inhibitor or stable expression of shRNA against NOTCH3 resulted in a

223 siRNA, underlining the general potential of stable expression of shRNA molecules as a long term ther

224 By combining cell cycle synchronization with stable expression of shRNA targeting either ERK1 or ERK2

225 We developed a retroviral system for stable expression of siRNA directed to the unique fusion

226 A delivery in vivo is a challenging problem, stable expression of siRNA, which targets oncogenic fusi

227 Stable expression of siRNAs specific for any one of thes

228 However, stable expression of Sirt3 M3 protein was consistently a

229 Stable expression of Skp2 in OVCAR3 cells diminishes the

230 These studies suggest that stable expression of small interfering RNA for STAT3 has

231 broblasts or by knockdown of this protein by stable expression of small interfering RNA in H460 cells

232 Stable expression of small interfering RNAs (siRNA) targ

233 A constitutive ERK2 mutant induces stable expression of Snail1, N-cadherin, and vimentin, a

234 Using stable expression of SPDEF in PC3-Luc cells and shRNA-me

235 Stable expression of STAT3 in GH3 cells induced expressi

236 Stable expression of STRAP results in activation of mito

237 horylation, which was insufficient to induce stable expression of T-bet.

238 a permanent transformation event, permitting stable expression of T-DNA-encoded transgenes.

239 Stable expression of TAF7 in TAF7-deficient cells restor

240 morphogens act combinatorially to induce the stable expression of target genes.

241 effector genes, demonstrating that outwardly stable expression of terminal effector genes in postmito

242 RA, through its receptor RARalpha, sustains stable expression of Th1 lineage specifying genes, as we

243 Stable expression of the -90 beta-ZF-DBD in mouse erythr

244 ut that not all positions of insertion yield stable expression of the added gene.

245 Stable expression of the Ah(b-2) receptor in the C57BL/6

246 Stable expression of the APRs in Arabidopsis (Arabidopsi

247 D(+) from the cytosol to the mitochondria by stable expression of the Arabidopsis thaliana mitochondr

248 had no effect on Stx susceptibility, whereas stable expression of the canine and murine FS resulted i

249 DOX strongly downregulated the stable expression of the CcO subunits II and Va and had

250 Conversely, a stable expression of the cDNA of hsp70 in the reverse or

251 Inducible stable expression of the COOH-truncated HBx protein (wit

252 Moreover, similar to wild-type DAL-1, the stable expression of the DAL-1 F359Y mutant significantl

253 based delivery system that allowed long-term stable expression of the desired siRNA.

254 We show that stable expression of the FAK C-terminal domain (termed F

255 On adaptation to mice, stable expression of the fluorescent proteins in infecte

256 more, intact phycobilisomes are required for stable expression of the flv4-2 operon genes and for the

257 Transient or stable expression of the full-length wild-type beta3A su

258 l shuttle vector that can be used to achieve stable expression of the library in mammalian cells for

259 Stable expression of the mitochondrial intermembrane pro

260 In this report, we demonstrate that stable expression of the myofibroblast phenotype require

261 The transient and stable expression of the p19 gene driven by a constituti

262 ested in tilapia, both through transient and stable expression of the reporter construct.

263 Stable expression of the reporter gene from its unique c

264 Stable expression of the ribozyme in human cells reduced

265 Stable expression of the siRNA resulted in approximately

266 Stable expression of the super-repressor, SR-IkappaBalph

267 al only occurred in models that incorporated stable expression of the target antigen.

268 gulatory T (Treg) cells are characterized by stable expression of the transcription factor Foxp3 and

269 Stable expression of the two mTOR mutants in NIH3T3 cell

270 Stable expression of the virally encoded latency-associa

271 man gene replacement because they facilitate stable expression of therapeutic proteins in transduced

272 Stable expression of these BMI1 mutants decreased ETOP-i

273 Furthermore, stable expression of this hVPS34 kinase-dead mutant caus

274 Following stable expression of this mEGFP-tagged receptor, Spatial

275 munoprecipitated with telomeric DNA and that stable expression of this protein in telomerase-positive

276 Here, we report that stable expression of TRAF2-DeltaR at a physiological lev

277 In addition, stable expression of TRAF2-DeltaR in TRAF2/5 DKO cells e

278 Moreover, stable expression of TRAF2-DeltaR in TRAF2/5 DKO cells f

279 ent protein-protein interactions (PPIs) into stable expression of transgenes are powerful tools for P

280 Inflammation eliminated previously stable expression of transgenes delivered by AAV; the li

281 re potent gene delivery vehicles that enable stable expression of transgenes in both dividing and pos

282 ylation pattern of the TRPL channel mediates stable expression of TRPL in the rhabdomeres upon prolon

283 Stable expression of truncated, hyperactive ASXL1-BAP1 c

284 n embryonic kidney (HEK) 293 cells that have stable expression of type 1 ryanodine receptor (RyR1).

285 ethylation inhibitor S-adenosylmethionine or stable expression of uPA short hairpin RNA significantly

286 We observed that stable expression of wild type PIPKIgamma661 in Madin-Da

287 Stable expression of wild-type GRK2 or a kinase-dead mut

288 serum-stimulated G(1)-phase progression, and stable expression of wild-type S6K1 confers a proliferat

289 rence) autotransporter translocator, and its stable expression on the surface of the bacterium was de

290 Stable expression or injection of peptides AKAP-IS or Ht

291 of known PMN ligands revealed a surprisingly stable expression pattern in hypoxia.

292 By contrast, high-NANOG cells show a more stable expression profile in different environments, wit

293 iated with bistability, characterized by two stable expression states that allow cells to respond to

294 nal transducer Gal3p is able to generate two stable expression states with a persistent memory of pre

295 total protein-coding transcripts displayed a stable expression, suggesting that the desiccation toler

296 We thus developed a stable expression system to express green fluorescent pr

297 In transient and stable expression systems PHV-AC2 expression was found t

298 ntropic retroviral transduction gave rise to stable expression that persisted at a high level for at

299 genicity, capability to achieve targeted and stable expression through integration, and low mutagenic

300 expressing p13(II), using both transient and stable expression vectors.