ライフサイエンスコーパス: stable transfection

1 lls overexpressing integrin alphavbeta3 upon stable transfection.

2 press moderate levels of the hDkk protein by stable transfection.

3 t construct are inserted into fibroblasts by stable transfection.

4 asts or overexpressed following transient or stable transfection.

5 ation was probably due to demethylation upon stable transfection.

6 d using NIH3T3 cells that express AGR2 after stable transfection.

7 ta in squamous cell carcinoma (SCC) lines by stable transfection.

8 n in both cell lines following transient and stable transfection.

9 ctional NCX1 was introduced in H9c2 cells by stable transfection.

10 colorectal cancer (CRC) cells (HT-29-EP4) by stable transfection.

11 tic adenocarcinoma cells were established by stable transfection.

12 gher efficiencies both for transient and for stable transfections.

13 chromocytoma (PC12) cells after transient or stable transfections.

14 compared with PC12 cells after transient or stable transfections.

15 lanoma cells, we show that upon transient or stable transfection a reporter gene is expressed in a Tc

16 llele by recombinant adenovirus infection or stable transfection also stabilized endogenous MDM2 in p

17 Introduction of AhR cDNA into AhR-D cells by stable transfection alters these characteristics such th

18 ed grp78 gene transcription as determined by stable transfection analyses with the rat grp78 promoter

19 Stable transfection and expression of constitutively act

20 Stable transfection and HYAL1v1-specific antibody confir

21 Upon stable transfection and induction of B. cereus sphingomy

22 cell lines expressing low levels of SATB1 by stable transfection and investigated its effect on stabl

23 cell line expressing IgG1-201 was derived by stable transfection and optimized for antibody secretion

24 We have previously demonstrated that stable transfection and overexpression of hIRS-1 in huma

25 Stable transfection and overexpression of IkappaBalpha i

26 Stable transfection and siRNA knockdown in MIN-6 cells/h

27 e elements (GREs) was performed initially by stable transfection and then with long-term transient tr

28 In this work, stable transfection and transgenic mouse assays have bee

29 ed2 gene introduced into MDA-MB-231 cells by stable transfection, and we found that mRNA containing t

30 Using a stable transfection assay we show that the region contai

31 Stable transfection assays additionally demonstrated dec

32 of the mouse bcl-x promoter by transient and stable transfection assays in a mouse erythroid cell lin

33 In stable transfection assays of murine endothelial progeni

34 We have used stable transfection assays to show that activation of th

35 1A sensitivity was apparent in transient and stable transfection assays, and E1A inhibited expression

36 tivity of HS2 by 40 and 38% in transient and stable transfection assays, respectively.

37 beta- or the gamma-globin gene promoters in stable transfection assays, the HS-40 core sequence was

38 , abolished transactivation in transient and stable transfection assays.

39 region functions as a chromatin insulator in stable transfection assays.

40 ment required for activity in transient- and stable-transfection assays bound Oct-1 and Oct-2, both o

41 From R- cells, we have generated (by stable transfection) cell lines with IGF-IR numbers rang

42 d with indomethacin and in dominant negative stable transfection clones.

43 In stable transfections, coexpression of the ODC dominant n

44 ed from a collagen alpha1(I) minigene and in stable transfections decreased the half-life of collagen

45 High, stable transfection efficiencies in human ES cells have

46 Stable transfection experiments enabled us to determine

47 f each pair expressed the E6 protein through stable transfection (HCT116/HCT116-E6, MCF7/MCF7-E6, and

48 CB1 cells, in which SPT has been restored by stable transfection, however, produce large amounts of 1

49 glycine 553 with leucine (G553L) followed by stable transfection in HEK 293 cells.

50 ird transmembrane domain were examined after stable transfection in HEK-293 cells.

51 ion of allelic variants of hGSTP1-1, through stable transfection in HepG2 cells, against (+)-anti-BPD

52 siently at high or at lower levels following stable transfection in LPA-nonresponsive RH7777 cells.

53 Following stable transfection in PC12 cells, which lack platelet-d

54 electrical activity and insulin secretion by stable transfection in the INS-1 cell line.

55 died after expression in Xenopus oocytes and stable transfection in the mammalian L929 cell line.

56 re, we have overexpressed human caspase-9 by stable transfection in the SNB19 glioblastoma cell line,

57 Transient and stable transfections in HeLa and K562 cells demonstrated

58 Transient and stable transfections indicated that the proximal promote

59 ancer (HuE lambda) on c-myc expression after stable transfection into BL cells.

60 By stable transfection into E-cadherin-deficient cell lines

61 After stable transfection into K562 cells, HS2 had strong enha

62 elements to signal de novo methylation upon stable transfection into mouse embryonal carcinoma cells

63 roduce a wild-type copy of the hPMS2 cDNA by stable transfection into the PMS2 mutant HEC-1-A cell li

64 that will undergo switch recombination upon stable transfection into the surface IgM+ B cell line (I

65 cuous activity following either transient or stable transfection into tissue culture cells, indicatin

66 Stable transfection of 22Rv1 cells with Hyal1 did not al

67 After stable transfection of 293 cells with wild-type and a mu

68 Stable transfection of 3T3L1 cells with miR-17-92 result

69 Stable transfection of a 2HG-producing mutant IDH into i

70 ls, which inherently overexpress CEACAM6, by stable transfection of a CEACAM6 small interfering RNA-g

71 Conversely, stable transfection of a constitutively active Src mutan

72 Stable transfection of a CRTAP or LEPRE1 expression cons

73 uppression of cyclin D1 expression after the stable transfection of a cyclin D1 antisense construct i

74 After stable transfection of a Cyr61 cDNA expression vector, p

75 After stable transfection of a DLK1 cDNA expression vector int

76 ch the function of p53 is impaired either by stable transfection of a dominant negative form of p53 o

77 Moreover, stable transfection of a dominant-negative p85 (subunit

78 Stable transfection of a dominant-negative transforming

79 Stable transfection of a gene encoding a soluble Mr 42,0

80 In the current study, stable transfection of a genomic HLA-A2 gene construct,

81 Stable transfection of a human colon carcinoma cell line

82 In this report we describe the stable transfection of a human pancreatic adenocarcinoma

83 Stable transfection of a kappaB-dependent reporter in A5

84 e activation of NF-kappaB was interrupted by stable transfection of a kinase-mutated form of IkappaB

85 Stable transfection of a luciferase reporter construct c

86 Stable transfection of a mutant IkappaBalpha lacking Ser

87 Stable transfection of a nestin antisense sequence into

88 Stable transfection of a new, chimeric reporter in the h

89 Stable transfection of a non-SMC cell type with Myocd el

90 We now report that stable transfection of a Pgp (pgp1) promoter/luciferase

91 d cell line using two different methods: (1) stable transfection of a plasmid containing PIG-A, and (

92 Furthermore, stable transfection of a related serine protease inhibit

93 the malignant phenotype was characterized by stable transfection of a single chain antibody (ScFv5R)

94 Stable transfection of a small interfering RNA (siRNA) t

95 Stable transfection of a tetracycline-regulatable rat RI

96 pha (TGF-alpha) was ectopically expressed by stable transfection of a TGF-alpha cDNA under repressibl

97 In this study it is shown that stable transfection of A1 into an interleukin-3 (IL-3)-d

98 either by exposure to chemical carcinogen or stable transfection of activated Ha-ras.

99 However, stable transfection of an approximately 620-kb yeast art

100 transformed in culture by a carcinogen or by stable transfection of an oncogene express Sp1 at 8-fold

101 In contrast, although stable transfection of an RII expression vector into the

102 By using stable transfection of antisense cDNAs, we studied the f

103 Stable transfection of antisense plasmids expressing the

104 tin-mediated adenocarcinoma cell adhesion by stable transfection of antisense sequences directed at t

105 nges in the levels of PDZ proteins following stable transfection of any HPV31 genomes into HFKs.

106 After stable transfection of ATDC5 cells with inducible sense

107 cking both beta-arrestins and is restored by stable transfection of beta-arrestin1.

108 Stable transfection of cells with antisense RNA targeted

109 CRSBP-1 overexpression (by stable transfection of cells with CRSBP-1 cDNA) enhances

110 Stable transfection of cells with dominant-negative casp

111 Stable transfection of CGA into a CGA-deficient pituitar

112 We expressed whole human IgG in vitro by stable transfection of Chinese hamster ovary cells with

113 Stable transfection of Chinese hamster ovary cells with

114 Stable transfection of Chinese hamster ovary cells, by c

115 G1 constant region (CD83Ig) and expressed by stable transfection of Chinese hamster ovary cells.

116 tially identical results were obtained after stable transfection of CHO cells or after injecting the

117 We report that stable transfection of clonal undifferentiated thyroid c

118 Stable transfection of COLO-357 human pancreatic cancer

119 However, after the transient or stable transfection of connexin43, volume change did occ

120 Stable transfection of COS-7 cells with deletion constru

121 Stable transfection of COS-7 cells with deletion constru

122 re highly cytotoxic to MCF-7 cells following stable transfection of CYP2B1 but exhibited no toxicity

123 /-) cells compared with daxx(+/+) cells, and stable transfection of daxx(-/-) cells with Daxx restore

124 Stable transfection of diabetic B cell lines with an Ii

125 ase (PI3K) inhibitor), an Akt inhibitor, and stable transfection of dominant negative-Akt1.

126 Stable transfection of dominant-negative mutant IkappaB

127 Stable transfection of EDG-1 into adult-medial VSMCs enh

128 Stable transfection of EGFR-negative NR6 and EGFR-positi

129 tablished several isogenic ER+ cell lines by stable transfection of ERalpha expression vectors into E

130 Stable transfection of ES cells, using a human growth ho

131 In a prior study, we have shown that stable transfection of expression plasmids for protein k

132 Stable transfection of FaDu with luciferase allowed us t

133 RK activation, was inhibited by transient or stable transfection of FAK related non-kinase (FRNK), kn

134 Similar to other FATPs, transient and stable transfection of FATP6 into 293 cells enhanced upt

135 ha2(I) (COL1A2) mRNA was inhibited following stable transfection of Fli-1 in dermal fibroblasts.

136 arcinoma cells form primitive endoderm after stable transfection of Frizzled-2 chimera and stimulatio

137 e manipulated in human colon cancer cells by stable transfection of galectin-3 antisense, short hairp

138 We showed previously that stable transfection of gamma(a) into NRK-52E cells resul

139 Stable transfection of GnT-V into human glioma U-373 MG

140 Stable transfection of GSTP1 into Raji cells decreased t

141 On the other hand, stable transfection of H1299 human lung carcinoma cells

142 After transient and stable transfection of HC11 cells, Id4 overexpression in

143 recombinant full-length human C1q involving stable transfection of HEK 293-F mammalian cells and fus

144 Stable transfection of HEK-293T cells with plasmid DNA c

145 Stable transfection of HEK293 cells with the cDNA encodi

146 The cell lines were prepared by stable transfection of HEK293S-TetR cells with expressio

147 t colonies by only approximately 30-45% upon stable transfection of HeLa cells.

148 src activity were produced by transient and stable transfection of Hep3B cells.

149 cer LNCaP cells can also be increased by the stable transfection of HER2/Neu.

150 Stable transfection of HRPAP20 into MCF-7 cells signific

151 Stable transfection of human breast carcinoma MCF-7 cell

152 This is based on stable transfection of human hepatoma HepG2 cells with a

153 on of foci, a condition also accomplished by stable transfection of IEC6 with a Wnt11-expressing cons

154 Stable transfection of IFITM3 inhibits OPN, which mediat

155 were rendered unable to regenerate cilia by stable transfection of IFT88-shRNA.

156 We used stable transfection of inducible constructs and transien

157 Overexpression of SMPD3 and NEFH by stable transfection of inducible constructs into an HCC

158 Stable transfection of INS-1res cells with a plasmid con

159 Here we report that stable transfection of Kir1.1b (ROMK2) in Madin-Darby ca

160 6 hepatocytes Gck expression was reduced and stable transfection of Klf6 led to up-regulation of Gck.

161 Transient or stable transfection of LAMA84 or K562 cells with a const

162 Stable transfection of LLC1 cells with a small interferi

163 rotein 1 (LSP1) on phagocytic cell motility, stable transfection of LSP1-null U937 cell line with an

164 ane translocation, and more specifically, by stable transfection of lung-metastatic MTF7L breast canc

165 After stable transfection of LXRalpha, As4.1 cells show a cAMP

166 Stable transfection of Maspin inhibited basal and TGFbet

167 Stable transfection of MCF-7 cells with a constitutively

168 Stable transfection of MCF-7 cells with a constitutively

169 Stable transfection of MCF-7 cells with the HMGA1b cDNA

170 Furthermore, stable transfection of McNtcp.24 cells with the compleme

171 Stable transfection of MDMX into U2OS cells does not alt

172 Stable transfection of murine 32D myeloid cells (which l

173 Expression of BCGs by stable transfection of murine neuroblastoma (F-11) cells

174 In parallel to the studies in CHO cells, the stable transfection of Myc-CK2alpha and Myc-CK2beta subu

175 y, we have examined Myf5 proteolysis through stable transfection of myogenically convertible U20S cel

176 Stable transfection of NHERF-1/EBP50 into OKH cells rest

177 Stable transfection of NIH 3T3 and MCF-7 cells with C32S

178 the AAH genes were assessed by transient and stable transfection of NIH-3T3 cells with human and muri

179 Stable transfection of NIH/3T3 cells with human L1 resul

180 Stable transfection of non-endothelial cells with AQP1 o

181 Stable transfection of parental MCF-7 cells with a domin

182 Here we show that stable transfection of parkin in the human dopaminergic

183 Stable transfection of PARP -/- fibroblasts with wild-ty

184 Stable transfection of PC3 and DU145 prostate cell lines

185 Inhibition of these enzymes by stable transfection of PC3M-LN4 cells with anti-sense HA

186 Stable transfection of PCAF caused an increase in TbetaR

187 s overexpressing HO-1 by either transient or stable transfection of pcDNA3.1/HO-1 construct.

188 ulation of ODC by oxidants was determined by stable transfection of PE cells with a dominant-negative

189 Stable transfection of PGM into PGM-deficient K562 leuke

190 Stable transfection of PR into mouse mammary epithelial

191 Stable transfection of primary cutaneous melanoma SB-2 c

192 Stable transfection of Rab5a cDNA into macrophages accel

193 In cancer cells, stable transfection of RARbeta exhibited strong inhibiti

194 Stable transfection of rat breast tumor cell lines with

195 Stable transfection of RCS-LTC cells with bovine ADAMTS-

196 ells in the presence of adenovirus; and (vi) stable transfection of recombinant AAV plasmids containi

197 Notably, stable transfection of retinoic acid receptor gamma rest

198 Stable transfection of RIIbeta in Jurkat T cells led to

199 Finally, the stable transfection of RRIG1 inhibited esophageal SCC ce

200 Stable transfection of SAAS cDNAs into AtT-20 cells was

201 In parallel, stable transfection of SC with dominant negative Asn-17

202 Stable transfection of SCC-112 cDNA in MDA-MB 231 breast

203 Stable transfection of Seg-1 with a Fas expression vecto

204 n-3 on MUC2 mucin production was assessed by stable transfection of sense and antisense galectin-3 ex

205 Stable transfection of short hairpin RNA was used to gen

206 Stable transfection of SOX4 into nontransformed prostate

207 Stable transfection of ST6GalNAcV into U373MG glioma cel

208 Moreover, stable transfection of TAP in LNCaP cells suppressed LNC

209 Importantly, stable transfection of the antiapoptotic baculovirus P35

210 pression of ganglioside GD3 was inhibited by stable transfection of the antisense vector against CMP-

211 After stable transfection of the cDNA into human embryonic kid

212 Stable transfection of the cells with a dominant negativ

213 ppropriate reporter gene expression required stable transfection of the constructs in cell lines, sug

214 on of the EGF-R in different cell types, and stable transfection of the EGF-R cDNA causes phosphoryla

215 These cell lines were produced by stable transfection of the estrogen receptor (ER) gene i

216 The stable transfection of the FucT-VII gene into Jurkat cel

217 Stable transfection of the full-length cDNA in the human

218 Stable transfection of the hPDGF-B AS in MCF-7/Neu cells

219 Stable transfection of the HRPAP20 cDNA into Nb2-11 cell

220 Stable transfection of the Mer chimera into interleukin

221 Stable transfection of the mutant c-kit complementary DN

222 Interestingly, stable transfection of the nm23-M2/NDPK-B del-RGD or G10

223 Stable transfection of the NQO1-deficient cell line, MDA

224 Stable transfection of the PBR-negative R2C Leydig cells

225 Furthermore, stable transfection of the PC-3 highly invasive cells wi

226 Stable transfection of the PDGF-Ralpha into Patch cells

227 Stable transfection of the SW620 metastatic colon cancer

228 Stable transfection of these cells with a glypican-1 ant

229 Stable transfection of these constructs into the Ba/F3 l

230 Stable transfection of these plasmids decreased VEGF-A m

231 Moreover, stable transfection of TSP2-null fibroblasts with mouse

232 Stable transfection of WEHI7.2 cells with a mutant form

233 Stable transfection of wild type ATM cDNA reversed these

234 Stable transfection of wild-type MAPK (Erk2) into P- cel

235 vivo mtDNA mutations, which are reversed by stable transfection of wild-type p53.

236 Stable transfection of wild-type PS1 but not PS2 correct

237 Stable transfection of wild-type Smad2, Smad3, or Smad4

238 We report that upon stable transfection of Wnt-1 into the mouse mammary epit

239 Stable transfections of betaPPT-A or NK1-Tr expression v

240 sion in early development, were tested using stable transfections of HEL and K562 cells.

241 halves to interact were explored using both stable transfections of Pgp mutants in mammalian cell li

242 d in transient transfections and 144-fold in stable transfections of the LNCaP prostate cell line.

243 ion of the MMP-2 proenzyme were modulated by stable transfections of tumor cells with cDNA encoding m

244 xpression of gelsolin in Gsn- fibroblasts by stable transfection or adenovirus reverts the ruffling r

245 eric genes were introduced into CHO cells by stable transfection or into Xenopus oocytes by microinje

246 progression, we overexpressed 14-3-3zeta by stable transfection or reduced 14-3-3zeta expression by

247 Overexpression of human BCL-2 in nuclei by stable transfection resulted in an inhibition of Ca2+-st

248 Stable transfection resulted in plasma membrane localiza

249 ring RIL expression in colon cancer cells by stable transfection resulted in reduced cell growth and

250 findings demonstrate that both transient and stable transfections resulted in a 2.5-fold higher expre

251 e MDA-231 cells were genetically modified by stable transfection so as to increase their production o

252 ructs can be prepared and tested relative to stable-transfection strategies.

253 ssed in rat aortic endothelial cells using a stable transfection system under the control of a cytome

254 In a stable transfection system, dominant-negative mutants of

255 Consideration of both transient and stable transfection systems suggests that in addition to

256 ed in mammalian cells by either transient or stable transfection, the fusion protein acts as a bona f

257 (shRNA) to knockdown exocyst expression and stable transfection to induce exocyst overexpression, we

258 ction) and templates organized as chromatin (stable transfection) to understand the effect of chromat

259 Our results from transient and stable transfections using luciferase reporter construct

260 ecombinant cell colonies) were observed with stable transfections, using co-transfected marker plasmi

261 Stable transfection was used to determine the effect of

262 either lacked pVHL or expressed pVHL through stable transfection were used to prepare RNA from cytoso

263 m5F beta-cell (Syt III cell) was achieved by stable transfection, which conferred greater Ca(2+) sens

264 Similarly, stable transfection with a dominant-negative mutant of c

265 n of either the function of EGR-1 protein by stable transfection with a dominant-negative mutant or t

266 red to p53-null PC3 prostate cancer cells by stable transfection with a human temperature-sensitive p

267 inhibition of ALVA-31 cells was abolished by stable transfection with a p21 antisense construct.

268 Clonal cell lines derived from stable transfection with a pOB4GFP target construct and

269 transfer and in H(9)C(2) cardiomyoblasts by stable transfection with a tetracycline-repressible UCP3

270 cells that express no detectable tTG due to stable transfection with a tTG antisense construct, ther

271 DNA-alkylating agents are rescued following stable transfection with a wild-type beta-polymerase min

272 d E1A following either infection with Ad5 or stable transfection with Ad5-E1A.

273 opes on these melanoma cells was achieved by stable transfection with alpha,3GT cDNA.

274 In contrast, stable transfection with an antisense connexin43 cDNA re

275 subclone rendered invasive and metastatic by stable transfection with an expression vector for OPN to

276 xpression was increased in MCF-7 cells after stable transfection with an HRG expression construct tha

277 dogenous CD23 expression was knocked down by stable transfection with CD23 shRNA retroviral plasmid.

278 rs (CaCO2) was significantly increased after stable transfection with CD47.

279 rofessional APC), induced by IFN-gamma or by stable transfection with CIITA, with constitutive class

280 ved from RIN 1046-38 rat insulinoma cells by stable transfection with combinations of genes encoding

281 I-FLICE antisense treatment was performed by stable transfection with complementary DNA (cDNA) for I-

282 Stable transfection with fibroblast growth factors (FGFs

283 ession of GRP94 is silenced via transient or stable transfection with GRP94-directed small interferin

284 a, we re-expressed ERalpha in C4-12 cells by stable transfection with HA-tagged ERalpha.

285 zed human prostate epithelial subline M12 by stable transfection with human wild-type AR cDNA.

286 Furthermore, stable transfection with ICSBP or IRF-1 construct inhibi

287 nylglycine t-butyl ester (DAPT) or following stable transfection with Notch1-short hairpin RNA (shRNA

288 ed by 93%, 74%, and 99%, respectively, after stable transfection with Nox4 siRNA relative to nontarge

289 Stable transfection with p53-dependent reporter construc

290 in PARP(-/-) fibroblasts and its loss after stable transfection with PARP cDNA.

291 Stable transfection with RacV12, a constitutively active

292 Knockdown of KHK by stable transfection with small hairpin RNA demonstrated

293 cted F-11A cells contain mainly GM3, whereas stable transfection with ST2 or GalNAcT results in the p

294 harbors constitutively activated EGFR after stable transfection with TGF-alpha cDNA.

295 embryonic kidney 293 cells made competent by stable transfection with the Epstein-Barr neutral antige

296 king p53 function were also prepared through stable transfection with the human papillomavirus type-1

297 duced by H. pylori in HEK293 cells following stable transfection with TLR2 or TLR5 expression plasmid

298 mpetent for H2O2-induced Erk activation upon stable transfection with wild-type ZAP-70.

299 NSC34 motor neuron-like cell line following stable transfection with Wld(S).

300 successfully transduced by AAV vectors after stable transfections with cDNAs encoding the murine HSPG