ライフサイエンスコーパス: steady-state kinetics

1 are efficient substrates for CCA addition in steady-state kinetics.

2 ation half-reaction that was not observed in steady-state kinetics.

3 and the resulting chimeras were subjected to steady-state kinetics.

4 a substrate were well described by solution steady-state kinetics.

5 ibition of the elongating ribosome using pre-steady-state kinetics.

6 nalysis and EPR spectroscopy, and studied by steady-state kinetics.

7 echanism of the PKCtheta kinase domain using steady-state kinetics.

8 hroism spectroscopy, and presteady-state and steady-state kinetics.

9 t and the mutant enzymes characterised using steady-state kinetics.

10 very similar to the k(cat) value measured by steady-state kinetics.

11 allosteric behavior gives rise to sigmoidal steady-state kinetics.

12 ith two coexisting conformations observed in steady-state kinetics.

13 mits study of the hydride transfer step with steady-state kinetics.

14 elta was examined using steady-state and pre-steady-state kinetics.

15 poldelta.dNTP binding was determined by pre-steady-state kinetics.

16 DNA complexes employing steady-state and pre-steady-state kinetics.

17 gnificant effect on either rapid kinetics or steady-state kinetics.

18 ve, tighter binding form observed in the pre-steady-state kinetics.

19 mational distribution, in agreement with the steady-state kinetics.

20 g isothermal titration calorimetry (ITC) and steady-state kinetics.

21 tential amino acid substrates and to measure steady-state kinetics.

22 racterized using fluorescence anisotropy and steady-state kinetics.

23 lysis of citryl-CoA that generally dominates steady-state kinetics.

24 fit the observed time dependence in the pre-steady-state kinetics, a model for dinucleotide synthesi

25 From steady state kinetics, addition of Q67H mutations does n

26 Single-turnover pre-steady-state kinetics also showed that the kpol of Pol a

27 Using steady-state kinetics analyses and molecular docking, we

28 Steady-state kinetics analysis confirmed the results obt

29 th increasing LTA4 concentrations during the steady-state kinetics analysis, indicating poor lipid su

30 fluorescence resonance energy transfer, pre-steady state kinetics and cryo-electron microscopy.

31 ATP or MgADP before APS) as established from steady state kinetics and equilibrium binding studies.

32 The four mutant enzymes were studied using steady state kinetics and heavy-atom isotope effects wit

33 In contrast, from both steady state kinetics and ITC studies, binding of DHF is

34 netic mechanism of Na(+)-NQR with the use of steady state kinetics and stopped flow analysis.

35 Using steady state kinetics and the pH dependence of kinetic p

36 Here, we employed pre-steady state kinetics and X-ray crystallography to estab

37 alone and with human APE1 (hAPE1), using pre-steady-state kinetics and a coupled-enzyme (hTDG-hAPE1)

38 f a two binding site model to interpret both steady-state kinetics and binding events.

39 Steady-state kinetics and biophysical studies show that

40 ith human MTAP was also characterized by pre-steady-state kinetics and by analysis of the crystal str

41 These mutants were characterized using steady-state kinetics and deuterium substrate and solven

42 -> Asn mutant exhibited significantly slowed steady-state kinetics and diminished product yields, sug

43 els have been proposed, these mainly rely on steady-state kinetics and do not provide information on

44 Steady-state kinetics and EPR experiments are consistent

45 re purified without affinity tags, and their steady-state kinetics and glucose binding affinities wer

46 Using steady-state kinetics and in vivo metabolic labeling stu

47 allowed rate constants to be determined from steady-state kinetics and independently determined chain

48 cterium smegmatis expression system, and the steady-state kinetics and inhibitory actions of phenothi

49 ng a combination of protein engineering, pre-steady-state kinetics and isothermal titration calorimet

50 l titration calorimetry, we investigated the steady-state kinetics and ligand binding properties of P

51 As judged by steady-state kinetics and mass spectrometry, bypass of p

52 Employing pre-steady-state kinetics and mass spectrometry, we determin

53 The deoxynucleotides were characterized via steady-state kinetics and PCR, while the ribonucleosides

54 s isolated and characterized with respect to steady-state kinetics and pre-steady-state burst magnitu

55 First, two-substrate steady-state kinetics and product inhibition patterns in

56 Steady-state kinetics and radioactive substrate binding

57 rate oxidation by choline oxidase using both steady-state kinetics and rapid kinetics techniques.

58 atalytic properties were characterized using steady-state kinetics and resonance Raman spectroscopy.

59 Ki values from steady-state kinetics and saturation kinetics were compa

60 t the subunit interface and, as confirmed by steady-state kinetics and site-directed mutagenesis, rev

61 Pre-steady-state kinetics and temperature-jump studies indic

62 ed high electron-accepting activity, and the steady-state kinetics and the NADH-Q2 oxidoreductase rea

63 Here we report pre-steady-state kinetics and time-dependent crystal structu

64 osity, and kinetic isotope effects (KIEs) in steady-state kinetics and time-resolved absorbance spect

65 scherichia coli was analyzed with respect to steady-state kinetics and tryptophan binding.

66 omain of the insulin receptor was studied by steady-state kinetics and x-ray crystallography.

67 ficant changes in the dNTP binding affinity, steady state kinetics, and fidelity of the enzyme.

68 or polymerization (kpol), as assessed by pre-steady-state kinetics, and it requires the RecQ C-termin

69 d using metal analyses, steady-state and pre-steady-state kinetics, and NMR and EPR spectroscopies to

70 ed mutagenesis, ligand-binding measurements, steady-state kinetics, and reaction product profiles est

71 n cofactor absorbance, secondary structures, steady-state kinetics, and sensitivity toward hydrogen p

72 These mutant enzymes were analyzed by steady-state kinetics, and the crystal structures of the

73 n using gel filtration, steady-state and pre-steady-state kinetics, and the pH dependence of inhibiti

74 variants that show increased sigmoidality in steady-state kinetics, and which are less sensitive to t

75 We have applied a pre-steady-state kinetics approach to address this question

76 Using a steady-state kinetics approach with purified proteins we

77 Through the use of a pre-steady-state kinetics approach, the NCp7-annealed primer

78 his paper, site-directed mutagenesis and pre-steady state kinetics are used to determine the roles th

79 ta when kinetic parameters from biphasic pre-steady-state kinetics are employed.

80 S-methylglutathione inhibition determined by steady-state kinetics are nearly 100-fold higher.

81 e investigate the concept of metabolic quasi-steady state kinetics as a means of applying metabolic c

82 ies including ligand binding, transient, and steady-state kinetics as well as product analysis by rap

83 een studied using laser flash photolysis and steady-state kinetics assays.

84 The steady-state kinetics associated with the oxidation of s

85 mouse isozymes were compared with respect to steady state kinetics, chain length of polyglutamate der

86 bits enhanced catalytic performance, and pre-steady-state kinetics characterization of the complete S

87 On the basis of steady-state kinetics, circular dichroism spectroscopy,

88 Steady-state kinetics combined with mass spectrometry of

89 Here, we use steady-state and pre-steady-state kinetics combined with mutagenesis and stru

90 The differences between the steady-state kinetics correlate well with the Lm redox p

91 limited the ability to obtain comparable pre-steady-state kinetics data at saturating concentration o

92 Second, our steady-state kinetics data revealed that the human E627K

93 Steady and pre-steady state kinetics demonstrated that XMRV RT is signi

94 Steady-state kinetics demonstrated that oxygen reacts be

95 Pre-steady-state kinetics demonstrates that turnover in TbQS

96 d kinetic properties of the complex and, via steady-state kinetics, derived a kinetic mechanism with

97 C) of Yersinia pestis was investigated using steady-state kinetics employing structural analogues of

98 kinetic mechanism using steady-state and pre-steady-state kinetics, equilibrium binding, and isotope

99 Pre-steady-state kinetics, examination of the elemental effe

100 - 0.5 and (D)k(cat) = 1.06 +/- 0.4), and pre-steady-state kinetics exhibited a discernible, but negli

101 Steady-state kinetics exhibited a pH dependence in k(cat

102 Pre-steady-state kinetics exhibited a single-exponential bur

103 end, we have performed steady-state and pre-steady-state kinetics experiments and have determined ra

104 Steady-state kinetics experiments and Lineweaver-Burk pl

105 Earlier results from pre-steady-state kinetics experiments have identified the ra

106 Steady-state kinetics experiments showed similar k(cat)

107 In addition, we used steady-state kinetics experiments to demonstrate that th

108 We used stopped-flow and steady-state kinetics experiments, along with single-mol

109 In steady-state kinetics experiments, CMPD1 was observed to

110 Using pre-steady-state and steady-state kinetics experiments, we also show that arc

111 The pre-steady state kinetics for binding glutamine to the binar

112 yltransferases in general, we determined the steady state kinetics for the hetero-octameric form and

113 Steady-state and pre-steady-state kinetics for helicase activity were monitor

114 Analysis of steady-state kinetics for modification of peptide substr

115 Steady-state kinetics for removal of methyl groups from

116 elity of base-pair synthesis was measured by steady-state kinetics for single-nucleotide insertions.

117 Changes in the steady-state kinetics for tetrahydrobiopterin (BH(4)) an

118 Steady-state kinetics for the Ag-substituted enzyme yiel

119 A strategy is developed to analyze steady-state kinetics for the hydrolysis of a soluble su

120 Pre-steady-state kinetics gave a linear rate of NAMN formati

121 A careful study of the enzyme's steady state kinetics has been carried out.

122 The temperature dependence of steady-state kinetics has been studied with horse liver

123 by single- and double-mutant variants using steady-state kinetics, high resolution X-ray crystallogr

124 pH, salt, NAD+, divalent cation profiles and steady-state kinetics.However, the former is more discri

125 is controversial whether the measurement of steady-state kinetics identifies cells that otherwise wo

126 inine, or glutamic acid residues changed the steady-state kinetics in a similar way.

127 ate specificity of any enzyme and to measure steady-state kinetics in an automated fashion.

128 scale as OAT (SIPr/MesCNO and IPr/N2O); (IV) steady-state kinetics in which no detectable intermediat

129 Pre-steady-state kinetics indicate that similar Na+ - or H+

130 However, steady-state kinetics indicate that TH has a different c

131 Steady-state kinetics indicated that the presence of at

132 )k(cat) = 1.6 +/- 0.2), and immeasurable pre-steady-state kinetics, indicating an extremely fast pre-

133 ad little effect on either the activation or steady-state kinetics, indicating that both the substrat

134 alue (1.09 +/- 0.05 s(-1)) obtained from the steady-state kinetics, indicating that glycine formation

135 However, the S402P mutant exhibits steady-state kinetics, indicating that the mutant is mor

136 In steady-state kinetics inhibition experiments performed w

137 The pre-steady-state kinetics is composed of a burst phase and a

138 Glucokinase steady-state kinetics is unaffected by the elongation of

139 Here, ligand binding, pre-steady state kinetics, isotope trapping, and isotope exc

140 Steady-state kinetics, isotope effects, and presteady-st

141 and characterized it using a combination of steady-state kinetics, isotope effects, isotopic labelin

142 e-directed mutagenesis, steady-state and pre-steady-state kinetics, ITC binding analysis, methanolysi

143 rders of magnitude as demonstrated using pre-steady-state kinetics (k(pol)/K(d,app)).

144 Steady-state kinetics, ligand-observe NMR, isothermal ti

145 Steady-state kinetics measurements on the soluble kinase

146 Pre-steady-state kinetics measurements reveal a 50-fold decr

147 Steady-state kinetics measurements show that complementa

148 Steady-state kinetics measurements showed that the 4-flu

149 Steady-state kinetics measurements were carried out to e

150 galactose oxidase, has been investigated via steady-state kinetics measurements, absorption, CD and E

151 re, we use mutagenesis, steady-state and pre-steady-state kinetics, motility assays, and electron par

152 ce outside the cavitand, displaying standard steady-state kinetics; no hemiaminal was observed.

153 The steady state kinetics of a Desulfovibrio (D.) vulgaris s

154 Further analysis of steady state kinetics of AKT1 in the presence of dead-en

155 Pre-steady state kinetics of enzyme.PRPP and enzyme.PRPP.glu

156 The steady state kinetics of exchange follows the ping-pong

157 cence changes can be used to monitor the pre-steady state kinetics of human tyrosyl-tRNA synthetase.

158 Quantitative evaluation of the steady state kinetics of MEK inhibition by these compoun

159 ation mechanism of Syk, we characterized the steady state kinetics of Syk substrate phosphorylation.

160 A can greatly shift both the steady and pre-steady state kinetics of the enzyme as well as the fidel

161 On the basis of steady state kinetics of the OxyBtei-catalyzed reaction,

162 In addition we characterize the steady state kinetics of this enzyme and report the firs

163 We show that the steady-state kinetics of a chemical reaction can be anal

164 The steady-state kinetics of a full-length and truncated for

165 l quench-flow methods to investigate the pre-steady-state kinetics of a protein-tyrosine phosphatase

166 MTSEA, MTSET, and MTSES all changed the pre-steady-state kinetics of A166C, independent of pH, and s

167 r to wild-type enzyme, which allowed the pre-steady-state kinetics of adenosylcobalamin homolysis to

168 The steady-state kinetics of aminoacylation by LysRS indicat

169 We monitored the pre-steady-state kinetics of ATP hydrolysis by RecQ and the

170 The steady-state kinetics of both wild-type and tunnel mutan

171 irst report of detailed steady-state and pre-steady-state kinetics of carbapenem hydrolysis, performe

172 To this end, we characterized the steady-state kinetics of Cet1p using both synthetic RNA

173 c properties, ligand binding affinities, and steady-state kinetics of chlorite dismutase from Dechlor

174 ofuran, were used for these studies, and the steady-state kinetics of correct nucleotide insertion we

175 This was used to study the pre-steady-state kinetics of covalent intermediate formation

176 clamp-loading reaction by measuring the pre-steady-state kinetics of DNA binding and ATP hydrolysis

177 The steady-state and pre-steady-state kinetics of DNA replication were measured w

178 variety of biophysical methods to probe the steady-state kinetics of fibrillar protein self-assembly

179 We have examined the steady-state kinetics of formation of base pairs opposit

180 ) = 2.3 +/- 0.1) but unlike Gly-Arg-AMC, pre-steady-state kinetics of Gly-Tyr-AMC and Ser-Tyr-AMC wer

181 The effects of vitamin B-6 status on steady-state kinetics of homocysteine metabolism in huma

182 establish whether vitamin B-6 status affects steady-state kinetics of homocysteine metabolism in the

183 This paper describes the steady-state kinetics of hydrolysis of a series of speci

184 Steady-state kinetics of incorporation for natural dNTPs

185 Steady-state kinetics of insertion for all possible misp

186 anistic approach was used to compare the pre-steady-state kinetics of KIF3AC to the kinetics of homod

187 lantoin is demonstrated, and analysis of the steady-state kinetics of KpHpxA yielded a k(cat)/K(m) of

188 No significant changes in the steady-state kinetics of LeuRS aminoacylation were obser

189 echanism and gamma subunit rotation, the pre-steady-state kinetics of Mg x ATP hydrolysis in the solu

190 here, comparison of the steady-state and pre-steady-state kinetics of MlTrxR-Trx with those of MlTrxR

191 The steady-state kinetics of NADH entering the immobilized p

192 ccounts for the observed presteady-state and steady-state kinetics of neutral dipeptide and drug tran

193 the basis of a rigorous analysis of the pre-steady-state kinetics of nucleotide binding and hydrolys

194 Pre-steady-state and steady-state kinetics of nucleotide incorporation and ex

195 rigor heads would have a large effect on the steady-state kinetics of one-head thiophosphorylated HMM

196 Steady-state kinetics of peptide phosphorylation were de

197 Using this assay, we measured the steady-state kinetics of peptides representing the H4 hi

198 Here, we determined the steady- and pre-steady-state kinetics of reverse glutamate transport wit

199 Steady-state kinetics of single nucleotide incorporation

200 Analysis of pre-steady-state kinetics of ternary complex formation revea

201 In this report, the pre-steady-state kinetics of the ATP and ATPgammaS-mediated

202 stoichiometric steps and their comparison to steady-state kinetics of the catalytic reaction reveal t

203 The pre-steady-state kinetics of the DM11-catalyzed addition of

204 The steady-state kinetics of the enzyme exhibit a pH optimum

205 is of caged glutamate, we determined the pre-steady-state kinetics of the glutamate-induced anion cur

206 The pre-steady-state kinetics of the half-reactions of several s

207 Pre-steady-state kinetics of the native enzyme toward glycid

208 Steady-state kinetics of the PfSET7 enzyme are similar t

209 The pre-steady-state kinetics of the reaction of beta-benzoyl-L-

210 The steady-state kinetics of the recombinant carboxyltransfe

211 The value for Em(EH2/EH4) obtained from the steady-state kinetics of the TrxR-catalyzed reaction bet

212 Analysis of the steady-state kinetics of these reactions indicate a tetr

213 In this study, we have analyzed the steady-state kinetics of these two types of reactions ca

214 The unusual steady-state kinetics of this zinc-containing enzyme ini

215 Determination of the steady-state kinetics of tRNA(Pro) charging showed that

216 Steady-state kinetics of truncated but dimeric MutS prot

217 n derived peptides, the regioselectivity and steady-state kinetics of tTGase-catalyzed deamidation of

218 Here, we describe the steady-state and pre-steady-state kinetics of urease inhibition by fluoride.

219 irected mutagenesis and steady-state and pre-steady-state kinetics on a representative member of this

220 Pre-steady-state kinetics on WT RT showed 14- and 53-fold hi

221 ined contributions of the two modules to the steady-state kinetics parameters for tryptophan-dependen

222 e-directed mutagenesis, steady state and pre-steady state kinetics, pH analysis, isotope partitioning

223 s in characterizing allosteric CYP behavior, steady state kinetics, product ratios, and ( k H/ k D) o

224 Steady-state kinetics, product inhibition, and isotope e

225 lved manner, we can successfully measure pre-steady-state kinetics, regardless of the incorporation o

226 rent dissociation constant of 37 microM, and steady-state kinetics reveal a competitive mode of inhib

227 alysis by both x-ray crystallography and pre-steady-state kinetics revealed an equilibrium between di

228 Steady-state kinetics revealed that dCTP incorporation i

229 ay structures of the common ancestor and pre-steady-state kinetics reveals a detailed atomistic mecha

230 Steady-state kinetics reveals substrate cooperativity fo

231 Steady-state kinetics show that RifR has a preference fo

232 The steady-state kinetics show that SLO-1 proceeds through a

233 Steady-state kinetics show that two groups need to be de

234 uman P450 21A2 with both substrates, and pre-steady-state kinetics showed a partial burst but only wi

235 Pre-steady-state kinetics showed faster rates of dCTP incorp

236 Steady-state kinetics showed full-length MPG has higher

237 Pre-steady-state kinetics showed no burst phase kinetics for

238 Pre-steady-state kinetics showed that the exonuclease rates

239 Steady state kinetics shows that human AMCase has "low"

240 Steady-state kinetics shows that FgFCO1 prefers alpha1,2

241 ation process by ACCO was investigated using steady-state kinetics, solvent isotope effects (SIEs), a

242 have been determined by a combination of pre-steady-state kinetics, solvent isotope incorporation, an

243 equiring acid-base catalysis, was studied by steady-state kinetics, spectral analyses of anaerobic st

244 rized using metal analyses, CD spectroscopy, steady-state kinetics, stopped-flow fluorescence, and fl

245 functional form in Escherichia coli and its steady state kinetics studied.

246 alyzed by human 3alpha-HSD type III by using steady-state kinetics studies and assessment of the abil

247 Previous steady-state kinetics studies have demonstrated a KIE of

248 Steady-state kinetics studies of 1 and 2 were carried ou

249 The model is suitable for steady-state kinetics such as fluorescence recovery afte

250 Steady-state kinetics suggest a compulsory ordered mecha

251 ry complex, which is further corroborated by steady-state kinetics, suggesting a possible feedback me

252 The pre-steady-state kinetics suggests that Mg(2+) at high but p

253 ed enzyme titration with the second pKa from steady-state kinetics suggests that this second pKa aris

254 rotein kinase 2 (MK2), have been explored by steady-state kinetics, surface plasmon resonance, isothe

255 the H285D variant of pol beta possesses pre-steady-state kinetics that are similar to the wild-type

256 with an analysis of the effect of p23 on the steady-state kinetics that revealed a mixed mechanism of

257 tion, we performed a detailed account of the steady-state kinetics, thermodynamics, molecular modelin

258 We used steady state and pre-steady state kinetics to characterize the minimal mechan

259 Here we employed pre-steady state kinetics to compare the kinetic responses t

260 amics simulations, NMR spectroscopy, and pre-steady-state kinetics to compare a mutator (H273R) RdRp

261 Here we use steady-state kinetics to determine the accuracy of DNA s

262 d C6PE or C6PS were selected and analyzed by steady-state kinetics to determine their specificity con

263 tal analyses, fluorescence spectroscopy, and steady-state kinetics to evaluate how these residues aff

264 Here, we use steady-state kinetics to examine nucleotide incorporatio

265 Here we have used pre-steady-state kinetics to examine the mechanisms of corre

266 We have used pre-steady-state kinetics to investigate the interaction of

267 Here, we use pre-steady-state kinetics to investigate the mechanism of nu

268 slower at low pH, permitting the use of pre-steady-state kinetics to measure the dissociation consta

269 we combine published data from transient and steady-state kinetics to model a minimal eight-state ATP

270 In steady-state kinetics, TPCK produced only slope effects

271 r tRNAAsp cleavage has been elucidated using steady-state kinetics, transient kinetics, and ligand af

272 Characterizing the steady state kinetics using a Syk kinase domain construc

273 Steady state kinetics using assays to study the first ha

274 Steady-state kinetics using substrates of various length

275 t, reactivity, as indicated by transient and steady-state kinetics, varies significantly and correlat

276 receptor's kinase domain were analyzed using steady-state kinetics, viscometric analysis, and equilib

277 The largest effect on the steady-state kinetics was observed with the C315 single

278 Using steady-state kinetics, we found that insertion of dCTP w

279 Using steady-state kinetics, we have identified crucial active

280 n of Bronsted analyses, pre-steady-state and steady-state kinetics, we have isolated all catalytic st

281 in studies of cellulase pre-steady-state and steady-state kinetics, we have prepared 4-methyl-7-thiou

282 Using steady-state kinetics, we have shown that halides are un

283 Using pre-steady-state kinetics, we show that Q does not inhibit t

284 Steady-state kinetics were evaluated for the 5A7A exonuc

285 Steady-state kinetics were examined using the enzyme at

286 Steady-state kinetics were measured with these mutant en

287 Thus, steady-state kinetics were modified to favour the open c

288 Pre-steady-state kinetics were performed to obtain rates of

289 (1)H NMR and steady-state kinetics were used to determine the regiosp

290 Site-directed mutagenesis and steady-state kinetics were used to identify three amino

291 ed in muscle tissue (M1-PK) shows hyperbolic steady-state kinetics, whereas PK expressed in kidney ti

292 ng bioluminescence emission spectroscopy and steady state kinetics with adenylate substrates.

293 sm, integration of both steady-state and pre-steady-state kinetics with sKIEs allowed the provision o

294 We characterized these lyase reactions by steady-state kinetics with the amino-terminal 8-kDa doma

295 is further supported by the KIEs, BIEs, and steady-state kinetics with the SAM analog Se-adenosyl-l-

296 Steady-state kinetics with the Y-family Sulfolobus solfa

297 Pre-steady-state and steady-state kinetics with two of these enzymes from Gra

298 fic and -selective substrates, as well as by steady-state kinetics with varying amounts of the substr

299 ADP and glucose-1-P follows ping-pong bi-bi steady state kinetics, with a k(cat) of 2.7 s(-)(1) and

300 e-directed mutagenesis, steady-state and pre-steady-state kinetics, X-ray crystallography, and modifi