ライフサイエンスコーパス: stepping

1 for scanning of one of the gratings ("phase stepping").

2 al ladder, a complex task requiring accurate stepping.

3 their involvement in control of accuracy of stepping.

4 I4 depolarization never elicited stepping.

5 es of inability to produce effective forward stepping.

6 across different cycle durations of neonate stepping.

7 lly limited by the need for mechanical phase stepping.

8 This conformation precedes ATP binding and stepping.

9 ontal ladder that required visual control of stepping.

10 ers and dimers as they bind MTs and initiate stepping.

11 generator, inducing active weight-supported stepping.

12 n of the system controlling the direction of stepping.

13 he aim of the patient achieving standing and stepping.

14 en stimulation parameters were optimised for stepping.

15 rgy provides a mechanism for processive back-stepping.

16 ipazine were given to each rat to facilitate stepping.

17 ankle of the rat to provide guidance during stepping.

18 dimer are coordinated to promote processive stepping.

19 e motor head binds to the microtubule during stepping.

20 fundamentally from that of plus-end-directed stepping.

21 minus end-directed to net plus end-directed stepping.

22 from minus end-directed to plus end-directed stepping.

23 xplain the accelerated triggering of induced stepping.

24 e the effectiveness of random, uncoordinated stepping: (1) the formation of three-headed and four-hea

25 For voluntary stepping, a disruption in the normal coupling between po

26 echanism and coordination of KIF3A and KIF3B stepping, a presteady-state kinetic analysis was pursued

27 s showed significant improvement in hindlimb stepping ability, quadrupedal weight support, and all me

28 e hindlimbs have been shown to improve their stepping ability.

29 During induced stepping, accelerated step initiation may represent an e

30 We propose that the minus end-directed stepping action of Cut7 is selectively inhibited by coll

31 r neighbors inhibit their minus end-directed stepping action, but not their plus end-directed steppin

32 ping action, but not their plus end-directed stepping action.

33 Finally, SNC-80 more potently stimulated stepping activity in mice lacking the NOP receptor than

34 ansmitters changes to achieve weight-bearing stepping after spinal cord injury.

35 As a control, we also tracked myosin V stepping along actin filaments and fascin-actin bundles.

36 at may account for the behavior of myosin VI stepping along actin.

37 ental data from GFP-labeled kinesin-1 motors stepping along immobilized microtubules, we show 1) that

38 kinesin-1 and kinesin-2 neck-linker chimeras stepping along microtubules in the absence or presence o

39 mer 'gate' each other to promote coordinated stepping along microtubules.

40 ed by cooperative action of molecular motors stepping along polar cytoskeletal elements.

41 Processive stepping along the long pitch helix of actin may provide

42 e the ability to switch protofilaments while stepping along the microtubule when challenged by an obs

43 ve functions are linked to delayed voluntary stepping, alterations in the coupling of posture and loc

44 Conversely, without force-dependent stepping, an unrealistically weak stator spring is requi

45 rface, a task that does not require accurate stepping and can be accomplished without the motor corte

46 errors in the parametric control of induced stepping and its effectiveness in stabilizing balance wo

47 s dual functionality, with microtubule-based stepping and regulation of microtubule dynamics.

48 he rhythmic output required for coordinating stepping and stabilizing the body axis during movements.

49 he rhythmic output required for coordinating stepping and stabilizing the body axis during movements.

50 elp delineate how interhead strain maximizes stepping and suggest that less processive kinesins are t

51 hin the complexes, due to asynchronous motor stepping and the resultant stretching of motor linkages,

52 ion of these complexes due to motor binding, stepping, and detachment were monitored by tracking the

53 lives, and several noted less clonus, easier stepping, and/or other improvements.

54 itions occur affects the probability of back-stepping; and 4), the existence of multiple equilibria i

55 A new hybrid time stepping approach, which couples the Runge-Kutta and New

56 Training improved the ability to generate stepping at a range of weight support levels, but normal

57 No signs of processive stepping at the single molecule level were observed in t

58 Pase rate, microtubule-binding affinity, and stepping behavior of dynein, and that microtubule bindin

59 -molecule microscopy to directly observe the stepping behavior of kinesin-1 and -2 family motors with

60 e consistent with a model wherein the normal stepping behavior of the myosin is supplemented by the p

61 e heads remained widely separated, and their stepping behavior varied as a function of interhead sepa

62 This stepping behavior was observed with a constitutively act

63 myosin II to exhibit evidence of processive stepping behavior.

64 ht bearing, step taking, and coordination of stepping behavior.

65 e parameterized via analyses of single-motor stepping behaviors, load-rate-dependent motor-filament d

66 standing dynein's directionality and unusual stepping behaviour.

67 is opportune case study describes visual and stepping behaviours of an 87 year old female (P8), both

68 eased fear or falling and maladaptive visual/stepping behaviours.

69 the model still shows processive directional stepping, but it becomes uncoordinated and the stepping

70 st understood of these is the hand-over-hand stepping by a swinging lever arm movement toward the plu

71 Here, we examined how base triplet stepping by RecA, Rad51, and Dmc1 is affected by DNA seq

72 at ATP hydrolysis in the bound head precedes stepping by the tethered head, leading to a mechanochemi

73 b CPGs and that the ability of SCA to induce stepping can be enhanced by the sacral CPGs.

74 lts, we propose a quantitative model for the stepping characteristics of dynein and its response to c

75 a range of weight support levels, but normal stepping characteristics were not restored.

76 lternated between two discrete values during stepping, corresponding to those intervals when the link

77 ics of structural transitions underlying the stepping cycle of kinesin-1 at physiological ATP, we use

78 rimental grounds, the repercussions of their stepping cycle on the collective dynamics still remains

79 aturating ATP, kinesin-1 spends half of each stepping cycle with one head bound, specifying a structu

80 the energy requirements for the fundamental stepping cycle, which likely includes energy from glycos

81 that trigger the two key transitions in the stepping cycle: detachment of the trailing head from the

82 cargos against load without coordinating the stepping cycles of its two heads.

83 r spinal cord transection, the generation of stepping depends on neurotransmitter systems entirely co

84 to individual motor domains in processively stepping dimers.

85 for full-length Cut7, the key determinant of stepping direction is the degree of motor crowding on th

86 yeast kinesin-5 motors, Cut7 can reverse its stepping direction, by mechanisms that are currently unc

87 s presented show that the kinesin processive stepping distance decreases 40-60% with EC50 values <100

88 Institute and Harvard Dental School, and his stepping down as editor of the "Discovery!" section of t

89 Eligibility for stepping down asthma controller medications included no

90 We sought to compare the safety and costs of stepping down asthma controller medications with maintai

91 Limited data exist regarding outcomes after stepping down asthma medication.

92 Stepping down asthma medications in those whose symptoms

93 95% or more of the Cyt c population folds by stepping down through the same set of foldon-dependent p

94 with the other factors to reduce chances of stepping down, but did not constitute a problem factor w

95 by phosphate release, determines the rate of stepping during a processive run because the rate of mic

96 ated with the ability to perform independent stepping during training.

97 an in vitro "tug of war" to characterize the stepping dynamics of single quantum-dot-labeled myoV and

98 mporal resolution imaging showed evidence of stepping dynamics of TCs regulated by nucleoskeleton and

99 germane to the findings of our Report: that stepping dynamics provided a better statistical descript

100 Quantifying the sequence dependence of XPD stepping dynamics with near base pair resolution, we pro

101 e-trial analysis of licking behavior reveals stepping dynamics with variable onset times, which is re

102 ous diffusion-to-bound dynamics or discrete "stepping" dynamics.

103 tease domain is to improve the translocation stepping efficiency of the helicase.

104 tral and peripheral stalks affects c-subunit stepping efficiency.

105 st product dissociation, and that isoprenoid stepping ensures short transit times.

106 contact inside it) correlated with absolute stepping error.

107 In permeabilized cells, stepping external [Ca(2+)] from 0 to 3 mum also produced

108 ons of this analysis include molecular-motor stepping, fluorophore bleaching, electrophysiology, part

109 rvation of enzymatic memory in translocation stepping, for the kinetics of translocation steps of dif

110 myoVI acts as an anchor to prevent myoV from stepping forward.

111 lated with, and in some cases predictive of, stepping frequency.

112 falling P8 consistently looked away from the stepping guide before completing the step into it in ord

113 cle, P8 generally visually fixated the first stepping guide until after foot contact inside it.

114 en asked to walk along a path containing two stepping guides positioned before and after an obstacle,

115 onding to random thermal fluctuations of the stepping head while it searched for its next actin-bindi

116 zed into a hierarchy that precisely controls stepping in a stereotypic, speed-dependent fashion.

117 tivity in the lumbosacral spinal cord during stepping in newborns, toddlers, preschoolers, and adults

118 f the track does not automatically instigate stepping in the absence of an additional stimulus (heat

119 ion type, inducing a local motion of either "stepping-in-place" or "single-step" type by bond interch

120 evocation of spontaneous and actively driven stepping, including optimization of these behaviors in d

121 Furthermore, a remarkable feature of newborn stepping is a higher overall activation of lumbar versus

122 During imbalance, stepping is a powerful protective action for preserving

123 e maximum speed, our model shows that stator-stepping is a thermally activated process with an energy

124 mechanism for this high processivity and how stepping is affected by force are unclear.

125 Newborn stepping is characterized by an alternating bilateral mo

126 g and falls, initiation slowing of voluntary stepping is observed together with perturbation-induced

127 epresent an event-triggering process whereby stepping is released according to the occurrence of a pe

128 the use of body-weight support in treadmill stepping, is a physical therapy intervention used to imp

129 Although our method still relies on phase stepping, it effectively uses only down to a single dete

130 rference (i.e., load-dependent modulation of stepping kinetics and binding-site exclusion), whereas f

131 Analysis of stepping kinetics in varying nucleotides shows that ATP

132 ttle or no user input for characterizing the stepping kinetics of molecular motors as recorded by opt

133 gs are consistent with recent studies of the stepping kinetics of the motor.

134 The randomness of stepping leads to a buildup of tension in the linkage be

135 nt study was designed to further compare the stepping-like movements generated via epidural (ES) and/

136 We examined the ability to generate stepping-like movements in response to ES and/or IS of s

137 relimb movements appeared first, followed by stepping-like movements in the hindlimbs.

138 of the spinal cord at L3-L7 induced hindlimb stepping-like movements on a moving treadmill belt, but

139 t neural mechanisms were activated to induce stepping-like movements.

140 face of the spinal cord) induced quadrupedal stepping-like movements.

141 in rotational mobility were detected during stepping, likely corresponding to random thermal fluctua

142 Back stepping may provide a mechanism to ensure efficient car

143 bined with long-term training, enhanced some stepping measures.

144 examine the structural dynamics of dynein's stepping mechanism in real time, we used polarized total

145 ting ATP (500 muM), we proposed a nonuniform stepping mechanism in which a UvrD monomer translocates

146 her, these observations demonstrate that the stepping mechanism of dynein is fundamentally different

147 many of the critical unknown aspects of the stepping mechanism of myosin 5 including head-head coord

148 ue form of motility suggests that myosin X's stepping mechanism takes advantage of the arrangement of

149 of stators is explained by a force-dependent stepping mechanism that is independent of the strength o

150 ctivity with functional implications for its stepping mechanism, and also reveal a previously unappre

151 rectly linked to the efficiency of kinesin's stepping mechanism, and other types of less efficient an

152 draw new conclusions on cytoplasmic dynein's stepping mechanism.

153 limited previous ensemble studies of a PcrA stepping mechanism.

154 f dynein is fundamentally different from the stepping mechanisms of other well-studied MT motors, bec

155 ells, we developed a rapid laser temperature stepping method capable of measuring complete thermal me

156 We describe an electromagnetic phase-stepping method that eliminates mechanical motion, thus

157 n transitions dynamically between the active stepping mode and this weak surface-associated mode enha

158 e window of evidence accumulation, and their stepping model cannot explain existing data as effective

159 ons we recorded were better described by the stepping model.

160 marine N cycle using a multicompartment time-stepping model.

161 red from this state, and then resumed normal stepping motility without detaching from the microtubule

162 Toward this end, single-molecule, in vitro stepping-motility assays are commonly used to determine

163 for the estimation of motility parameters in stepping-motility assays.

164 ular tension sensing is observed in dynein's stepping motion at high interhead separations.

165 he geometry, we propose a diffusive sideward stepping motion of Kip3 on the microtubule lattice, asym

166 synthase (FOF1) is comprised of two rotary, stepping motors (FO and F1) coupled by an elastic power

167 rs convert the energy of ATP hydrolysis into stepping movement along microtubules.

168 that SC stimulation evoked well coordinated stepping movements at different treadmill directions.

169 Human stepping movements emerge in utero and show several mile

170 limb controllers, which are able to generate stepping movements in different directions.

171 of walking is determined by the direction of stepping movements of individual limbs in relation to th

172 ast, MLR stimulation evoked well coordinated stepping movements only if the treadmill was moving in t

173 that enables sensory input from standing and stepping movements to serve as a source of neural contro

174 2), Our model with back-stepping naturally explains the observed absence of a ba

175 at the conditional knock-outs used alternate stepping, not hopping, during overground locomotion, sug

176 ence thus far that forward, single-base pair stepping of a helicase utilizes the spontaneous opening

177 appropriate specialist supervision, and the stepping of care based on systematic measurement of outc

178 ding proteins (for example, single-base-pair stepping of DNA translocases) along with the detection o

179 ndamentally distinct from the hand-over-hand stepping of kinesin and myosin.

180 teps in both directions, indicating that the stepping of multiple motors is correlated.

181 Processive stepping of myosin Va (myoV) has been tracked by monitor

182 tion states that results from the cycling or stepping of the applied potential.

183 In contrast to other processive motors, stepping of the dynein motor domains (heads) is not prec

184 synthase, and explain the need for the finer stepping of the F1 motor in the mammalian system, as see

185 ing of how ATP binding is coupled to forward stepping of the motor.

186 we avoid the computationally demanding time stepping of the partial differential equations that are

187 including the use of body-weight support in stepping on a treadmill, was not shown to be superior to

188 vity during more automated behavior, such as stepping on a treadmill.

189 the walker's center-of-mass will facilitate stepping on target footholds.

190 transfer from simple locomotion to accurate stepping on the ladder slow-conducting PTNs more profoun

191 ransition from simple locomotion to accurate stepping on the ladder, slow-conducting PTNs respond in

192 ses ATP hydrolysis, like other kinesins, for stepping on the microtubule lattice, but at the plus end

193 rmly attached to one microtubule while it is stepping on the other.

194 learn to avoid a tone-signaled footshock by stepping onto a nearby platform.

195 ass platform; forelimb use was unbiased when stepping onto the glass surface but biased when stepping

196 pping onto the glass surface but biased when stepping onto the other side.

197 Stepping out of an aggressively air-conditioned building

198 Hence, studying culture requires stepping out of it.

199 f the PPC of cats walking on a treadmill and stepping over a moving obstacle whose speed of advance w

200 Second, Dmc1 stepping over infrequent mismatches is likely critical f

201 plastic changes that may be adaptive in non-stepping paraplegia after SCI.

202 e complete taxonomy of MyoV's load-dependent stepping pathways, and the extent to which these are con

203 mbers translocate nucleosomes with a similar stepping pattern maintained by the catalytic subunit of

204 structure fully consistent with the variable stepping pattern observed as dynein moves along the MT.

205 In contrast to the variable stepping pattern of full-length myoVa, the myoVa-Mlph co

206 The irregular stepping pattern of MyoVc, which may result from flexibi

207 epping, but it becomes uncoordinated and the stepping pattern shows a greater variability, which repr

208 motor, and a new mode with a highly variable stepping pattern suggestive of altered gating.

209 between a strictly gated and hand-over-hand stepping pattern typical of a constitutively active moto

210 ding and twisting, as well as changes in the stepping pattern, but did not lead to falling.

211 loss of inputs to one eye by altering their stepping pattern.

212 the broad step-size distribution of the MVI stepping pattern.

213 speed and spatiotemporal characteristics of stepping persist.

214 its a wide repertoire of pathways during the stepping process, which is intimately connected to its b

215 el of precision to an otherwise noisy dynein stepping process.

216 Regardless of which motor wins, its stepping rate slows due to the resistive load of the los

217 otease domain also significantly reduces the stepping rate, but it doubles the processivity.

218 ciates at 2.1 s(-1), 20-fold slower than the stepping rate, demonstrating the presence of rear head g

219 d mant-ADP release is 5-fold faster than the stepping rate.

220 f myoVa leads to a high sensitivity of motor stepping rates to strain.

221 location as a sequence of coupled hydrolysis-stepping reactions.

222 on below the lesion are thus involved in the stepping recovery after an incomplete SCI.

223 This leaves the accuracy of dynein stepping relatively inaccurate and susceptible to therma

224 ed independently from other core features of stepping, revealing a striking freedom in an otherwise p

225 wever, because sensory feedback entrains the stepping rhythm, it is difficult to reveal central patte

226 g (LRC), phase-locking between breathing and stepping rhythms, occurs in many vertebrates.

227 otein from syncytial embryos, which we named Stepping stone (Sstn).

228 symmetric, sparing the basal ganglia, and a stepping stone appearance in the frontal pericallosal re

229 , we show that wood has served not only as a stepping stone between habitats but also as a bridge bet

230 ia intermediate unprotected habitats through stepping stone dispersal.

231 These results provide a stepping stone for engineering more complex nitrogen-ato

232 f molecular recorders, providing a necessary stepping stone for future biological work.

233 power of single-cell RNA-seq and provides a stepping stone for future explorations of cellular heter

234 The study provides a stepping stone for future models of transcriptional regu

235 dules have human orthologs, which provides a stepping stone for the identification of candidate genes

236 ct map has become in recent years, a crucial stepping stone for the prediction of the complete 3D str

237 Hopefully, this review can be used as a stepping stone for the reader wanting to gain a clearer

238 llocation, and this work provides a valuable stepping stone in guiding policy on CSF surveillance and

239 ere that family living acted as an essential stepping stone in the evolution of cooperative breeding

240 erature and argue that these could provide a stepping stone into the identification of scientific bes

241 itment at the single cell level, a potential stepping stone to improved lineage manipulation through

242 This work represents a stepping stone toward a better understanding of the WAF

243 Altogether, our results present the first stepping stone toward a better understanding of ubiquity

244 As a stepping stone towards chronic cellular monitoring, we d

245 (adding up to 206 nucleotides), an important stepping stone towards RNA self-replication.

246 Our work constitutes a stepping stone towards the paradigm of self-correcting q

247 This approach provides a stepping stone towards the rational engineering of molec

248 Our work may be used as a stepping stone towards using the HyperCEST technique as

249 alysis confirms the role of the alkene as a "stepping stone" for electron transfer.

250 explained by oceanic long-distance and maybe stepping-stone dispersal rather than vicariance.

251 sian steppes by fitting a spatially explicit stepping-stone model to genotype data from >300 horses s

252 ude much of the MPA network that may provide stepping-stone protection for species that must shift th

253 In a first step, we simulated the historical stepping-stone spreading dynamics of 40 marine alien spe

254 cing greenhouse gas emissions and a possible stepping-stone towards renewable synthetic fuels.

255 erve as an alternative, or even evolutionary stepping-stone, to switching between multiple systems.

256 some of the deleterious mutations served as stepping stones across otherwise impassable fitness vall

257 ionists are already advocating corridors and stepping stones as important conservation tools under cl

258 om land and indicate that islands may act as stepping stones facilitating the transoceanic transport

259 volution, mainly by intermittently providing stepping stones for island colonization.

260 fic and Indian oceans and propose islands as stepping stones for the transoceanic transport of terres

261 points should now be viewed as obstacles or stepping stones is a matter of perspective.

262 Together, these form the stepping stones that are expected to accelerate standard

263 iption factor-DNA interactions are important stepping stones towards a mechanistic understanding of m

264 opulations are only connected via additional stepping stones was supported by contemporary migration

265 Milu searched for vacant habitat patches as "stepping stones" and established refuge populations.

266 els provide intermediate states that act as "stepping stones" for electrons tunnelling across the dio

267 rient distributors, as well as evolutionary "stepping stones" for the deep sea biota.

268 l, persisting populations, which can act as "stepping stones" that enable a rapid population expansio

269 d animals were subject to chance in finding "stepping stones".

270 ed to, patterns like corridors and chains of stepping stones), and properties that affect a species'

271 ns requires intermediate habitats ("phantom" stepping stones).

272 " pattern, resembling corridors or chains of stepping stones.

273 th increasing charge stabilization on their "stepping stones." The presence of mobile anions facilita

274 Thus, ribosomal mutations can serve as stepping-stones in an evolutionary path leading to the e

275 he complexity of blood coagulation, they are stepping-stones towards further understanding and engine

276 Using a precision stepping task and synchronizing target visibility to the

277 Our work reveals features of base triplet stepping that are conserved among these three phylogenet

278 try (SCV), current transients acquired after stepping the potential along the redox wave of interest

279 e faradaic current response that occurs upon stepping the potential sufficiently past the standard el

280 was reactivated for further Pt deposition by stepping the potential to more positive values, where H(

281 When stepping the voltage to 0 mV, novel kinetic phases of ve

282 ysis finds that a number of proteins fold by stepping through a reproducible sequence of native-like

283 l triggering and other changes in protective stepping to impact fall risk.

284 Stepping towards this goal of incorporating biochemical

285 Non-stepping training also exacerbated some SCI-driven plast

286 eck-linker element that is crucial for motor stepping undergoes discrete, ordered displacements.

287 mmensurate response to the stress intensity, stepping up from a strategy based on tolerating the effe

288 Scientists are stepping up like never before to support science in the

289 rovide evidence for the clinical benefits of stepping up patients with COPD from an inhaled corticost

290 tion of molecular motor properties including stepping velocity and characteristic run length.

291 y and membrane viscosity, reaching up to the stepping velocity of single motors.

292 ks that were specifically trained (standing, stepping, volitional leg movement).

293 plete section (T13) in these cats, bilateral stepping was seen on the next day, a skill usually acqui

294 To examine the role of the NL in regulating stepping, we investigated NL mutants of various lengths

295 cid neck linker domain underlies coordinated stepping, we investigated the role of neck linker length

296 To resolve rotations during stepping, we used optical tweezers combined with an opti

297 The parameters of stepping were determined from global fitting of the two

298 Instead, we find that hTpx2 inhibits hKif15 stepping when microtubule-bound.

299 minimal ATPase cycle), the model reproduces stepping with 8-nm steps (even though the motor itself i

300 teral left hemisection (T10), cats recovered stepping with both hindlimbs within 3 weeks.