ライフサイエンスコーパス: stopped-flow

1 solved fluorescence decay every 0.1 ms after stopped flow.

2 tentiometric transition time with respect to stopped flow.

3 Stopped-flow absorbance analyses of the reaction of the

4 lanine complexes with O(2) were monitored by stopped-flow absorbance spectroscopy and rapid quench me

5 Iron oxidation experiments using stopped-flow absorbance spectroscopy revealed an extreme

6 2-NrdF, NrdI(hq), and O2 has been studied by stopped flow absorption and rapid freeze quench EPR spec

7 Stopped-flow absorption and isotope effect experiments h

8 vity of the Cu(I) site was probed by EPR and stopped-flow absorption spectroscopies, and a rapid one-

9 ir reactivity with O2 and NO was analyzed by stopped-flow absorption spectroscopy and amperometric me

10 With stopped-flow absorption spectroscopy, we detected and co

11 Also, anaerobic stopped-flow analyses revealed that the equilibrium diss

12 QR with the use of steady state kinetics and stopped flow analysis.

13 this complex process has been achieved using stopped-flow analysis but, due to limitations in instrum

14 Stopped-flow analysis demonstrated ordered assembly of H

15 Pre-steady state stopped-flow analysis of Escherichia coli d-3-phosphogly

16 Stopped-flow analysis of GTP-binding and GDP/GTP exchang

17 calorimetry, surface plasmon resonance, and stopped-flow analysis, we demonstrate that Ca(2+) binds

18 By rapid kinetic stopped-flow analysis, we identified the molecular mecha

19 Using stopped flow and other spectroscopic techniques, the the

20 oxylases was investigated in single turnover stopped flow and quench flow measurements of biotin tran

21 ng the fluorescence intensity and anisotropy stopped-flow and analytical ultracentrifugation methods.

22 enase (cis-CaaD) has now been examined using stopped-flow and chemical-quench techniques.

23 Stopped-flow and electrochemical measurements have been

24 oscopy, using conventional spectrometers and stopped-flow and laser-flash techniques.

25 ction was investigated using single turnover stopped-flow and quench-flow assays.

26 Stopped-flow and rapid-quench flow experiments of HadA w

27 Stopped-flow and steady-state experiments revealed that

28 Using stopped-flow and steady-state fluorescence methods, kine

29 We used stopped-flow and steady-state kinetics experiments, alon

30 onance Raman spectroscopy, pulse radiolysis, stopped flow, and electrospray ionization mass spectrome

31 ciation of these proteins using rapid-mixing stopped flow, and examine how the kinetic behavior is pe

32 Detailed electrochemical, stopped-flow, and NMR studies of the Co(III)-OH to Co(IV

33 chemical trapping of reaction intermediates, stopped-flow, and substrate hydrogen isotope exchange te

34 A home-built stopped-flow apparatus is interfaced to a Hadamard trans

35 studying such systems use stirred cuvettes, stopped-flow apparatus, microfluidic systems, or other s

36 as to optical data obtained with the present stopped-flow apparatus.

37 asuring the peak currents (Ip) produced in a stopped flow approach.

38 med by nanosecond laser-flash-photolysis and stopped-flow are accompanied by resonance Raman and FT-I

39 DNA polymerase activity was measured by a stopped-flow assay that monitors polymerase extension us

40 The fluorescence stopped-flow assay, therefore, provides a high-throughpu

41 robust sequential-mixing fluorescence-based stopped-flow assay.

42 Equilibrium and stopped-flow binding assays and size exclusion chromatog

43 X and other CA isoforms was determined via a stopped-flow, CA-catalyzed CO2 hydration assay.

44 Stopped-flow CD revealed that the time of unfolding of m

45 ination of real-time kinetic information via stopped-flow circular dichroism with steady-state data f

46 ep required 1 min at 1.6 V vs. Ag/AgCl under stopped flow conditions, which was preceded by a 10 s ac

47 Stopped flow data and luminescence resonance energy tran

48 Stopped-flow data show that, upon the initiation of the

49 Our stopped-flow data showed that the 420-nm intermediate wa

50 Pre-steady state kinetic data obtained in a stopped-flow device show that the initial binding of JBP

51 ever, traditional enzyme mixing methods like stopped-flow do not allow for the observation of fast pr

52 Consistent with this analysis, stopped flow experiments find betaine mostly affects DHF

53 Using quench flow and stopped flow experiments in a biochemical system for pro

54 reactivity are investigated by double-mixing stopped-flow experiments for both complexes.

55 its complex with DNA were analyzed by using stopped-flow experiments in which fluorescence changes i

56 Stopped-flow experiments indicate a pK(a) = 10.4 for DMS

57 Stopped-flow experiments revealed a approximately 2.7-fo

58 Stopped-flow experiments showed that pyrrole-based inhib

59 Anisotropy stopped-flow experiments showed that the kinetics descri

60 Stopped-flow experiments then demonstrated that the chan

61 We used quantum chemical computations and stopped-flow experiments to evaluate a chemical mechanis

62 by nondenaturing gel electrophoresis and by stopped-flow experiments using fluorescence-labeled CaM

63 Stopped-flow experiments using the dye RH421 show that F

64 Single turnover stopped-flow experiments were used to identify catalytic

65 Furthermore, concentration-dependent stopped-flow experiments with both factors reveal positi

66 Pre-steady-state, stopped-flow experiments with cefoxitin revealed a burst

67 en determined by a combination of mixing and stopped-flow experiments with spectrophotometric monitor

68 oliposome water permeability with the aid of stopped-flow experiments yielded a unitary water permeab

69 electron paramagnetic resonance, and optical stopped-flow experiments, along with calculations using

70 In stopped-flow experiments, we observed that cYY binding t

71 Using stopped-flow experiments, we show that RFS-1/RIP-1 confe

72 mumol.m(-2).s(-1) for various conditions in stopped-flow experiments.

73 lography, quantum chemical calculations, and stopped-flow experiments.

74 chanism of PvdA was determined by absorption stopped-flow experiments.

75 spectroscopic (optical, EPR), and kinetics (stopped-flow) experiments on variants in which residue T

76 and K48-linked IQF-diubiquitin by USP2 using stopped-flow florescence under a single-turnover conditi

77 We conducted stopped-flow fluorescence analyses of the binding of mon

78 -association using dynamic light scattering, stopped-flow fluorescence and circular dichroism spectro

79 Contradicting this assumption, we present stopped-flow fluorescence and NMR experiments that give

80 RNase footprinting, in vitro binding and stopped-flow fluorescence annealing assays showed that a

81 ystem was measured by circular dichroism and stopped-flow fluorescence as a function of pH and concen

82 berculosis rrnAP3 ribosomal promoter using a stopped-flow fluorescence assay.

83 Using two stopped-flow fluorescence assays that we developed previ

84 Using stopped-flow fluorescence data for the target associatio

85 Stopped-flow fluorescence experiments revealed that kine

86 Stopped-flow fluorescence experiments showed that anneal

87 rmotoga maritima CheA was investigated using stopped-flow fluorescence experiments that monitored bin

88 Stopped-flow fluorescence measurements of a tryptophan i

89 Stopped-flow fluorescence measurements with Mycobacteriu

90 In this study, using a stopped-flow fluorescence method, we examined the kineti

91 Here, using the stopped-flow fluorescence method, we measured the target

92 NA complex, using laser temperature jump and stopped-flow fluorescence methods with U1A proteins labe

93 Stopped-flow fluorescence resonance energy transfer expe

94 Lastly, stopped-flow fluorescence spectrophotometry showed that

95 Fourier-transform infrared and high-pressure stopped-flow fluorescence spectroscopies were applied.

96 Using stopped-flow fluorescence spectroscopy to measure associ

97 e KIS native-state equilibrium obtained from stopped-flow fluorescence studies of refolding His-tagge

98 Here we present a stopped-flow fluorescence study of the membrane-interact

99 of temperatures and pressures by means of a stopped-flow fluorescence technique.

100 Stopped-flow fluorescence techniques were used to determ

101 ciated with interconversion and unfolding by stopped-flow fluorescence to determine transition-state

102 pid quench and domain closure as measured by stopped-flow fluorescence were similar and asymmetric wi

103 We used sedimentation assays, stopped-flow fluorescence, and fluorescence microscopy t

104 sing steady-state and stopped-flow kinetics, stopped-flow fluorescence, and rapid-freeze-quench EPR.

105 DNA was detected by EMSA, steady-state, and stopped-flow fluorimetry.

106 m thermoautotrophicum homologue (MthK) and a stopped-flow fluorometric assay for fast channel activat

107 tants for sugar binding measured directly by stopped-flow fluorometry implicates k(off) as a major fa

108 we use isothermal titration calorimetry and stopped-flow fluorometry to determine and analyze the th

109 teoliposomes and urea efflux was measured by stopped-flow fluorometry to determine the urea transport

110 Here, we used stopped-flow Forster resonance energy transfer to invest

111 Here, we applied stopped-flow Fourier transform infrared and electron-nuc

112 on of electron paramagnetic resonance (EPR), stopped flow freeze quench on a millisecond-second time

113 Stopped-flow FRET and fluorescence anisotropy show that

114 Stopped-flow FRET studies indicated that such frustrated

115 For the first time, our stopped-flow FRET studies revealed that a DNA polymerase

116 ng translocation in real time using ensemble stopped-flow FRET with ribosomes containing fluorescent

117 physics approach, incorporating rapid-mixing stopped-flow, high-pressure, and CD spectroscopies.

118 The system utilizes a conventional stopped-flow injection system coupled to a modified low

119 absorption peak within the dead time of the stopped-flow instrument, likely the ketimine of pyridoxa

120 presented through kinetics measurements (by stopped-flow IR spectroscopy), X-ray crystal structure a

121 By means of NMR, stopped-flow IR, and quenched-flow techniques, the kinet

122 Stopped flow kinetic analysis was used to confirm this p

123 Stopped flow kinetic studies of the OAT reactions show a

124 tingly, isothermal titration calorimetry and stopped-flow kinetic analyses reveal uncoupled nucleotid

125 nt (17)O NMR spectroscopy, electrochemistry, stopped-flow kinetic analyses, and EPR measurements were

126 Stopped-flow kinetic analysis of the DypB-catalyzed reac

127 Stopped-flow kinetic data demonstrate flavin oxidation w

128 Stopped-flow kinetic data were obtained for the iron-typ

129 Rapid-scanning stopped-flow kinetic experiments demonstrated that subst

130 Stopped-flow kinetic experiments showed that the rate of

131 ine species compared to SHV-1 as detected by stopped-flow kinetic experiments under single-turnover c

132 imit of accuracy for equilibrium titrations, stopped-flow kinetic experiments were used to measure th

133 As shown through stopped-flow kinetic experiments, electron transfer capa

134 Using a combination of steady-state and stopped-flow kinetic experiments, substrate analogs, and

135 Stopped-flow kinetic investigations of soluble methane m

136 Stopped-flow kinetic measurements of sugar binding with

137 Rapid stopped-flow kinetic measurements show that increasing h

138 hanism of induced folding using fluorescence stopped-flow kinetic measurements to distinguish between

139 Forster resonance energy transfer (FRET) and stopped-flow kinetic measurements, we monitored Ca(2+)-i

140 nding to ssDNA by equilibrium titrations and stopped-flow kinetic measurements.

141 near diffusion-limited on rates, as shown by stopped-flow kinetic measurements.

142 k(CPET) values with those from prior thermal stopped-flow kinetic studies gives data sets for the oxi

143 Stopped-flow kinetic studies indicate that increased fle

144 Stopped-flow kinetic studies involving alterations in th

145 Here we report stopped-flow kinetic studies of azide binding to human f

146 Stopped-flow kinetic studies of the reaction of diferrou

147 Flavin fluorescence and stopped-flow kinetic studies on CaM-bound enzymes sugges

148 Stopped-flow kinetic studies on UTP binding followed by

149 Stopped-flow kinetic studies show that the oxidative tra

150 Stopped-flow kinetic studies suggest that the rate-deter

151 = 3,5-Me(2)C(6)H(3)) with O(2) was shown by stopped-flow kinetic studies to result in the rapid form

152 In stopped-flow kinetic studies with alpha,beta-methylenead

153 Stopped-flow kinetic studies, examining the impact of tr

154 y detectable C4a-(peroxy)flavin formation in stopped-flow kinetic studies.

155 second-order rate constants, measured using stopped-flow kinetic techniques, spanned 4 orders of mag

156 Transient stopped-flow kinetic traces at various final TMAO concen

157 city and high affinity for iNOS, using rapid stopped-flow kinetic, gel filtration, and spectrophotome

158 Stopped flow kinetics were used to measure the equilibri

159 side-chain groups in bulk measurements using stopped-flow kinetics and NMR spectroscopy.

160 Single-molecule microscopy and stopped-flow kinetics assays were carried out to underst

161 leotide conditions and carrying out parallel stopped-flow kinetics assays.

162 Stopped-flow kinetics at variable acidities in H(2)O and

163 Single-turnover stopped-flow kinetics experiments demonstrate that incre

164 We report stopped-flow kinetics experiments to study the folding a

165 Here we dissect the E*-E equilibrium with stopped-flow kinetics in the presence of excess ligand o

166 Stopped-flow kinetics of sugar binding by WT LacY in det

167 Stopped-flow kinetics revealed that the reduction of VAO

168 Stopped-flow kinetics showed slower unfolding at around

169 Stopped-flow kinetics studies monitoring the change in t

170 l analysis of these mutations based on rapid stopped-flow kinetics to determine elongation rates and

171 e combined molecular dynamics simulation and stopped-flow kinetics with fluorescence detection to tra

172 haracterized by single-turnover kinetics and stopped-flow kinetics with fluorescent detection.

173 Crystallography, stopped-flow kinetics, quench-flow reactions, and infect

174 e reaction mechanism, using steady-state and stopped-flow kinetics, stopped-flow fluorescence, and ra

175 ty similar to that of p-Ets, was examined by stopped-flow kinetics.

176 ycloserine was performed by pre-steady-state stopped-flow kinetics.

177 In this latter case, stopped-flow light scattering analysis reveals the trans

178 Osmotic water permeability was measured by stopped-flow light scattering in human and rat erythrocy

179 enal cortical membrane vesicles, measured by stopped-flow light scattering, was reduced in CAII-defic

180 Kinetics obtained from stopped-flow measurements are corroborated by current pl

181 mic parameters obtained from low-temperature stopped-flow measurements are in excellent agreement wit

182 Additionally, stopped-flow measurements have shown that the rate of ve

183 Experimental data obtained from stopped-flow measurements of the refolding of Escherichi

184 FRET-based stopped-flow measurements revealed that Atg18 rapidly ol

185 Stopped-flow measurements revealed that dye hydrophobici

186 Stopped-flow measurements show that the formation of the

187 Targeted amino acid mutagenesis and stopped-flow measurements substantiate the functional re

188 Stopped-flow measurements suggest that initial PROPPIN-m

189 In this work, we have performed fluorescence stopped-flow measurements to investigate the kinetics of

190 High-pressure stopped-flow measurements were performed at pH 8, which

191 Fluorescence stopped-flow measurements were used to determine rate co

192 Fluorescence intensity and anisotropy stopped-flow measurements were used to determine rate co

193 ough isothermal titration calorimetry (ITC), stopped-flow measurements, mutagenesis studies, and mole

194 separation properties were characterized by stopped-flow measurements.

195 n-rate is too fast to detect by conventional stopped-flow measurements.

196 eir ECD spectra have been recorded online by stopped-flow measurements.

197 than it bound large unilamellar vesicles in stopped-flow measurements.

198 t with steady state data confirming that the stopped-flow method used was appropriate for the reactio

199 with nitrones were determined using a UV-vis stopped-flow method, and phenyl radical (Ph(*)) trapping

200 10 has been examined, using the fluorescence stopped-flow method.

201 ding and dissociation kinetics determined by stopped-flow methods demonstrated that although actin gl

202 have developed single-turnover fluorescence stopped-flow methods that allow us to quantitatively exa

203 In this work, we used stopped-flow methods to monitor the coupling of adenosin

204 Fluorescence stopped-flow methods were used to record the kinetics of

205 Using single-turnover fluorescence stopped-flow methods, here we report that ClpA, when ass

206 further, we undertook kinetic studies using stopped-flow methods.

207 s are too fast to measure using conventional stopped-flow methods.

208 Next, using stopped-flow mixing coupled with far-UV circular dichroi

209 cation of spectrophotometric monitoring with stopped-flow mixing has been used to explore the role of

210 ormed transient time-resolved FRET following stopped-flow mixing of actin with labeled myosin, preinc

211 We employed a stopped-flow mixing technique to systematically investig

212 ay scattering (SAXS) in combination with the stopped-flow mixing technique, the entire micelle format

213 troscopy, and folding reactions initiated by stopped-flow mixing were monitored by fluorescence.

214 ECD spectra were measured in the stopped-flow mode and compared with results from quantum

215 torr Xe in 500 cc) in either single-batch or stopped-flow mode, negating in part the usual requiremen

216 Stopped-flow NMR measurements suitable for determination

217 Application of the stopped-flow NMR method to the study of the kinetics of

218 the conjugation of absorption spectroscopy, stopped-flow, NMR, and X-ray crystallography.

219 s where the dimeric species predominates, by stopped flow, observing prominent electrostatic sensitiv

220 equent characterizations by a combination of stopped-flow optical absorption spectroscopy and freeze-

221 luorescent nucleotides without using a rapid stopped-flow or quench-flow instrument and the generally

222 In this report, stopped-flow polarization was utilized to determine the

223 An electroporation apparatus hyphenated with stopped-flow sample injection permits the introduction o

224 n into DrDps2 was investigated by static and stopped-flow SAXS measurements, revealing dynamic struct

225 2, alpha2, CDP, and ATP has been examined by stopped-flow (SF) absorption and rapid freeze quench ele

226 unctional analysis of ET using high-pressure stopped-flow, solvent, and temperature perturbation stud

227 Using stopped-flow spectrofluorimetry, association rate consta

228 netic events associated with iron release by stopped-flow spectrofluorimetry, in the presence and in

229 Stopped flow spectrofluorometry analysis of recombinant

230 on reaction occurs in the mixing time of the stopped-flow spectrophotometer when arginine is the subs

231 ages during their reaction with DPPH using a stopped-flow spectrophotometer-based method.

232 troscopy of the oxidative half-reaction in a stopped-flow spectrophotometer.

233 Moreover, stopped-flow spectrophotometric experiments with the nNO

234 Stopped-flow spectrophotometric monitoring of the oxidat

235 lamine (DEA) has been investigated using the stopped-flow spectrophotometric technique at 25.0 degree

236 d alkanolamines have been investigated using stopped-flow spectrophotometry and (1)H NMR measurements

237 Based on results from stopped-flow spectrophotometry, the reduced enzyme-3HB c

238 oxylase (ABDC) was studied by rapid-scanning stopped-flow spectrophotometry.

239 molecule was kinetically characterized using stopped-flow spectrophotometry.

240 ption at 25 degrees C was investigated using stopped-flow spectrophotometry.

241 Stopped-flow spectroscopic data reveal that H(4)F accele

242 Stopped-flow spectroscopic data revealed that transfer o

243 Here, we use stopped flow spectroscopy to show that the CTD of Escher

244 To address this, we used stopped-flow spectroscopy and computer modeling approach

245 Using stopped-flow spectroscopy and laser-triggered NO release

246 x1)-dependent peroxidase assays conducted by stopped-flow spectroscopy demonstrated that V(max,app) i

247 ange of saturated fatty acids (C12-C20), and stopped-flow spectroscopy indicates a rapid reaction bet

248 Stopped-flow spectroscopy results indicated rapid initia

249 data and THF dissociation rates measured by stopped-flow spectroscopy shows that product release can

250 In this study, we employed difference stopped-flow spectroscopy to characterize reaction inter

251 Stopped-flow spectroscopy was employed to elucidate the

252 Stopped-flow spectroscopy was used to measure the bindin

253 idized and reduced flavin can be detected by stopped-flow spectroscopy, consistent with the expectati

254 Using stopped-flow spectroscopy, we determined rate constants

255 By employing stopped-flow spectroscopy, we have carried out a compara

256 Using double-mixing stopped-flow spectroscopy, we investigated the reactions

257 ociation and dissociation were determined by stopped-flow spectroscopy, yielding a k(f) and k(b) of (

258 ic parameters obtained from ITC, UV/vis, and stopped-flow spectroscopy.

259 d in the presence of SBDS using fluorescence stopped-flow spectroscopy.

260 rate the nickel(II) complex was monitored by stopped-flow spectroscopy.

261 The stopped-flow studies revealed a complete reaction pathwa

262 Stopped-flow studies using these two distinct tools reve

263 As estimated from stopped-flow studies, the rate of sugar-induced opening

264 Here, we present the first multimixing stopped-flow study on a fully functional truncated varia

265 nd I and compound II state in a multi-mixing stopped-flow study.

266 inetic-spectrophotometric data acquired by a stopped-flow system for the quantitation of tartrazine i

267 An additional modification leads to a stopped-flow system very efficient for instance for 2D N

268 Using the stopped-flow technique, we found that the binding reacti

269 anions, as evaluated by pulse radiolysis and stopped flow techniques.

270 benzoic acid (p-NBA) were investigated using stopped-flow techniques at 4 degrees C.

271 We used spectroscopic and stopped-flow techniques to further investigate how the c

272 with certain substrates has been observed by stopped-flow techniques when [(Ph3P)CuH]6 is treated wit

273 Using stopped-flow techniques, we systematically compare the r

274 ess the binding to MCL-1 using time-resolved stopped-flow techniques.

275 plex formation by fluorescence titration and stopped-flow techniques.

276 d using static binding, chemical quench, and stopped-flow techniques.

277 ansient kinetics approaches (quench-flow and stopped-flow) to determine how subunit-specific mutation

278 )N4(6-Me-DPEN))(O2)](+) (4), is observed (by stopped-flow) to rapidly and irreversibly form in this r

279 as sampled by Fpg, as evident from the F110W stopped-flow traces, but less extensively than oxoG.

280 This enabled a stopped-flow type of experiment to measure the (initiall

281 Stopped-flow UV-vis absorption coupled with rapid-freeze

282 by the reduced T201 variants was explored by stopped-flow UV-vis and Mossbauer spectroscopy.

283 Employing stopped-flow UV-vis methods, reactions were triggered by

284 ectivity for CO(2) over H(+) was observed by stopped-flow UV-vis spectroscopy of [Re(bipy-tBu)(CO)(3)

285 hosphine (PAr3) substrates were monitored by stopped-flow UV-vis spectroscopy, and revealed second-or

286 Rapid-mixing stopped-flow UV-vis studies show that the low-temperatur

287 In contrast, the kinetics (via stopped-flow UV-vis) for complex 3-O displayed a signifi

288 ed-valence CuA and its M160SeM derivative by stopped-flow UV-vis, EPR, and XAS at both Cu and Se edge

289 Herein we report stopped-flow UV-visible (UV-vis) spectroscopy, X-ray cry

290 ls and detected with the use of rapid-mixing stopped-flow UV-visible (UV-vis) spectroscopy.

291 Stopped-flow UV-visible absorption and freeze-quench Mos

292 ivity of recombinant human CBS by static and stopped-flow UV-visible absorption spectroscopy.

293 e role(s) that tyrosyl radicals play in DHP, stopped-flow UV-visible and rapid-freeze-quench EPR spec

294 The OER is also examined with stopped-flow UV-visible spectroscopy, and its kinetic be

295 oxyferrous states using biochemical assays, stopped-flow UV-visible, and rapid-freeze-quench electro

296 ts catalytic cycle using biochemical assays, stopped-flow UV-visible, resonance Raman, and rapid free

297 Experimental measurements from stopped-flow UV/vis spectrophotometry afforded derivatio

298 roteo mass spectrometry and conventional and stopped-flow UV/visible spectroscopy, was used in conjun

299 ence on either side of LacY were measured by stopped flow with LacY in detergent or in proteoliposome

300 rates of substrate binding were measured by stopped-flow with purified LacY either in detergent or i