ライフサイエンスコーパス: strand exchange

1 the ATP hydrolysis to work (in this case DNA strand exchange).

2 represents an intermediate in the process of strand exchange.

3 hat it is required upstream of RecA-mediated strand exchange.

4 egrees about the flat exchange interface for strand exchange.

5 tion of homologous joint molecules (JMs) and strand exchange.

6 on and signalling or promote ATP-independent strand exchange.

7 ional DNA repair through homology search and strand exchange.

8 1-Tid1 tilt the bias toward interhomolog DNA strand exchange.

9 leavages of the donor template necessary for strand exchange.

10 search for homologous DNA sequences and DNA strand exchange.

11 ons for the mechanism and preferentiality of strand exchange.

12 ncrease the directionality and stringency of strand exchange.

13 is an ATPase that mediates recombination via strand exchange.

14 ved recombinase, catalyses homology-directed strand exchange.

15 ration dependence parallels that seen in DNA strand exchange.

16 nd breaks (DSBs) to create the substrate for strand exchange.

17 ning reaction between DNA partners, yielding strand exchange.

18 s Rad51 homologue, SsoRadA, to stimulate DNA strand exchange.

19 ngle-stranded tails necessary for subsequent strand exchange.

20 DNA (ssDNA), but not dsDNA, to stimulate DNA strand exchange.

21 current models linking ATP hydrolysis to DNA strand exchange.

22 is is completely uncoupled from extended DNA strand exchange.

23 but is insufficient for accommodating donor strand exchange.

24 l separation of DNA pairing and extended DNA strand exchange.

25 major role in, and may be required for, DNA strand exchange.

26 As are paired and available for extended DNA strand exchange.

27 d polymer on single-stranded DNA to catalyze strand exchange.

28 results in stimulation of RAD51-promoted DNA strand exchange.

29 ences that are located distal to the site of strand exchange.

30 th both Rad52 and Rad51 and stimulated Rad51 strand exchange.

31 ight coupling between ATP hydrolysis and DNA strand exchange.

32 ent on DNA double-strand break formation and strand exchange.

33 binds RAD51, the enzyme responsible for DNA strand exchange.

34 f UvsX-ssDNA filaments that is active in DNA strand exchange.

35 gle-stranded DNA for recombinase loading and strand exchange.

36 y the archaeal RecA homolog RadA to catalyze strand exchange.

37 overhang that serves as a substrate for DNA strand exchange.

38 ecA from DNA and inhibited RecA-mediated DNA strand exchange.

39 ment in promoting homologous pairing and DNA strand exchange.

40 rases, which bind to the ends and facilitate strand exchange.

41 meric protein assembly that is competent for strand exchange.

42 Rad51 from dsDNA, the product complex of DNA strand exchange.

43 ATP inhibits strand exchange.

44 onal switch" that occurs prior to the second strand exchange.

45 extended DNA structure bound by RecA during strand exchange.

46 filament, in the direction of RecA-mediated strand exchange.

47 aments to a conformation more proficient for strand exchange.

48 eins are responsible for homology search and strand exchange.

49 specificity in a manner that stimulates DNA strand exchange.

50 t DNA double-strand break repair via inverse strand exchange.

51 2-MND1 as a 'molecular trigger' of RAD51 DNA strand exchange.

52 ure able to promote homology recognition and strand exchange.

53 in the B core site is required for the first strand exchange.

54 promote joint molecule formation to initiate strand-exchange.

55 the overlap regions between the sites of the strand exchanges.

56 ic complex and orchestrates the order of DNA strand exchanges.

57 In strand exchange a single-stranded DNA (ssDNA) bound to R

58 Rad51 mediates DNA strand exchange, a key reaction in DNA recombination.

59 Rad51 mediates DNA strand exchange, a key reaction in DNA recombination.

60 In vitro, HOP2-MND1 stimulates the DNA strand exchange activities of RAD51 and DMC1.

61 of Srs2, providing a means for tailoring DNA strand exchange activities to enhance the fidelity of re

62 in is a novel inhibitor of RecA-mediated DNA strand exchange activities.

63 In vitro, RAD52 has ssDNA annealing and DNA strand exchange activities.

64 onally, BLM has its own strand-annealing and strand-exchange activities.

65 1 (B02), which specifically inhibits the DNA strand exchange activity of human RAD51.

66 rprisingly, we found that BLM stimulates DNA strand exchange activity of RAD51.

67 The UvsX-H195Q mutant retains weak DNA strand exchange activity that is inhibited by wild-type

68 -stranded DNA (ssDNA) and showed more robust strand exchange activity with oligonucleotide substrates

69 ilament remodeling, fail to stimulate RAD-51 strand exchange activity, demonstrating that remodeling

70 DNA) molecules with high efficiency, and has strand exchange activity.

71 with reduced ssDNA-binding affinity and DNA strand-exchange activity in both H195Q and H195A mutants

72 When RPA could bind, it displayed its own strand-exchange activity.

73 ment has long been known to occur during DNA strand exchange, although its importance to this process

74 h this, we show that FANCJ can inhibit RAD51 strand exchange, an activity that is likely to be import

75 actions between hRad54 and hRad51 during DNA strand exchange and branch migration, which are two core

76 However, RadB does not catalyse strand exchange and does not turn over ATP efficiently.

77 characteristic dsDNA extension rates due to strand exchange and free RecA binding are the same, sugg

78 RAD54 promotes RAD51-mediated DNA strand exchange and has been described to both stabilize

79 domain of PapF in functions involving donor strand exchange and hierarchical assembly.

80 Rad51, for DNA binding, filament stability, strand exchange and interaction with the antirecombinase

81 recombination by making an initial cleavage, strand exchange and ligation, followed by strand swappin

82 lap region, followed by the second cleavage, strand exchange and ligation.

83 by a sequence identity-independent cleavage, strand exchange and ligation.

84 n vitro, RAD54 stimulates RAD51-mediated DNA strand exchange and promotes branch migration of Hollida

85 xpression of Rad51, a protein central to DNA strand exchange and recombination, did not further incre

86 e recombinases cut all strands in advance of strand exchange and religation.

87 ly depends on both RecA-catalyzed homologous strand exchange and RuvABC-catalyzed Holliday junction r

88 ze kinetically trapped, metastable states in strand exchange and strand displacement reactions for bu

89 tive binding interactions may play a role in strand exchange and supercoil unwinding activities of th

90 mechanism normally blocks multiple rounds of strand exchange and triggers product release after a sin

91 J isomerization then allows a second pair of strand exchanges and thus formation of the final recombi

92 WINKLE: strand-separation, strand-annealing, strand-exchange and branch migration suggest a dual role

93 discuss how RdgC might inhibit RecA-mediated strand exchange, and how RdgC might be displaced by othe

94 the RecA protein, including DNA binding, DNA strand exchange, and LexA protein cleavage.

95 in concert with SsbA and DprA, catalyzes DNA strand exchange, and SsbB is an accessory factor in the

96 The second strand exchange appears to be homology-dependent.

97 chanisms of RAD-51-DNA filament assembly and strand exchange are well characterized, the subsequent s

98 l RecA-like recombinase enzymes catalyze DNA strand exchange as elongated filaments on DNA.

99 tly published structural data that implicate strand exchange as part of a mechanism for IKK2 activati

100 to be critical to facilitate dimerization by strand exchange as well as dimer flexibility.

101 rotein requires ATP for the catalysis of DNA strand exchange, as do all Rad51 and RecA-like recombina

102 sites that flank the region of cleavage and strand exchange, as well as six arm-type sites.

103 Strand-exchange assays were performed specifically to as

104 recombination occurs preferentially as four-strand exchanges at similar locations between both pairs

105 sfer to study the mechanism of Dmc1-mediated strand exchange between DNA oligonucleotides with differ

106 cA homologs Rad51 and Dmc1 are essential for strand exchange between homologous chromosomes during me

107 It polymerizes onto DNA and promotes strand exchange between homologous chromosomes.

108 d51 in eukarya and RadA in archaea) catalyse strand exchange between homologous DNA molecules, the ce

109 tein of HR, possesses a unique activity: DNA strand exchange between homologous DNA sequences.

110 We show that in eukaryotes, inverse strand exchange between homologous dsDNA and RNA is a di

111 tion with wild type Flp, Flp(R191A) promotes strand exchange between MeP- and P-DNA partners.

112 Although providing an efficient rate of DNA strand exchange between polymorphic alleles, Dmc1 must a

113 h BRCA2 protein stimulates DMC1-mediated DNA strand exchange between RPA-ssDNA complexes and duplex D

114 The rate of strand exchange between the oligonucleotides increased 8

115 inases (RecA, Rad51, RadA and UvsX) catalyse strand-exchange between homologous DNA molecules by util

116 r Rad51 nucleoprotein filament formation and strand exchange, but ATP hydrolysis is not required for

117 All three variants are proficient in DNA strand exchange, but G151D is slightly more sensitive to

118 for DNA repair, including RAD51 mediated DNA strand exchange, but is dispensable for DNA replication.

119 and ssDNA active in homology search and DNA strand exchange, but the precise role of its ATPase acti

120 The resolvase Sin regulates DNA strand exchange by assembling an elaborate interwound sy

121 dies established that Brh2 can stimulate DNA strand exchange by enabling Rad51 nucleoprotein filament

122 of the ssDNA strand that is displaced by DNA strand exchange by Rad51 and RPA, to a second ssDNA stra

123 Only the first group enhances DNA strand exchange by RAD51.

124 networks, leading to rapid and efficient DNA strand exchange by Rad51.

125 se was intentionally slowed, consistent with strand exchange by random walk in which rate declines pr

126 t for sequence identity within the region of strand exchange, called the overlap region.

127 processive "360 degrees rotation" rounds of strand exchange can be observed, if the recombining site

128 Our results show how RNA strand exchange can expand the utility of RNAi computing

129 a much more central role in DNA pairing and strand exchange catalyzed by DrRecA than is the case for

130 activities of RecA that are important to DNA strand exchange, consistent with its role in targeting o

131 but not SsbB, DprA was able to activate DNA strand exchange dependent on RecA . ATP.

132 the peptide and its use as a tool to dissect strand exchange-dependent DNA repair within cells.

133 The defining step of HR is homologous strand exchange directed by the protein RAD51, which is

134 The products of extended DNA strand exchange do not form.

135 Namely, HOP2-mediated strand exchange does not require ATP and, in contrast to

136 ereas most mismatches near the 5' end impede strand exchange dramatically.

137 Nte) of an incoming pilus subunit by a donor-strand exchange (DSE) mechanism.

138 ered to the outer membrane usher where donor strand exchange (DSE) replaces PapD's donated beta stran

139 c recombinase responsible for initiating DNA strand exchange during homologous recombination.

140 ion of RAD51 from heteroduplex DNA following strand exchange during homologous recombination.

141 We propose that strand exchange during recombination events within guani

142 on of DNA partners and the directionality of strand exchange during recombination mediated by tyrosin

143 are four core sites that flank the sites of strand exchange during recombination.

144 that there is a strong bias in the order of strand exchanges during integrative recombination.

145 Topo I could play an active role in strand exchange, either by altering the kinetics or ther

146 drolysis is not required for DNA pairing and strand exchange, eliminating active search processes.

147 contrast, RecA . dATP efficiently catalyzes strand exchange even in the absence of single-stranded b

148 locus, and provide insights into aspects of strand exchange events between paralogous sequences in t

149 o different dominant-negative alleles of the strand exchange factor, Rad51.

150 e-stranded DNA was generated; the homologous strand-exchange factor, Rad51, accumulated into foci; a

151 sequences called loxP after which a pair of strand exchanges forms a Holliday junction (HJ) intermed

152 unbinding of non-homologous dsDNA and drives strand exchange forward for homologous dsDNA.

153 This differential extension drives strand exchange forward for homologs and increases the f

154 less extended incoming strand, which drives strand exchange forward.

155 f HIV-1 nucleocapsid protein, which promotes strand exchange, had little effect on this outcome.

156 irement for RecA filament disassembly in DNA strand exchange has a variety of ramifications for the c

157 s align homologous sequences and promote DNA strand exchange has long been known, as are the crystal

158 After strand exchange, heteroduplex dsDNA is bound to site I.

159 ive, small interfering RNA molecules via RNA strand exchange in a cell-free Drosophila embryo lysate,

160 ng and point toward the possibility of using strand exchange in a native biological setting.

161 ecombinase Dmc1 plays a critical role in DNA strand exchange in budding yeast.

162 al Rad51 paralogs that cooperate to catalyse strand exchange in eukaryotes.

163 These proteins normally promote DNA-DNA strand exchange in homologous recombination.

164 It enables RAD51 DNA strand exchange in the absence of divalent metal ions re

165 e loxP site are able to dictate the order of strand exchange in the Cre system.

166 DprA facilitates RecA-mediated DNA strand exchange in the presence of both SSB proteins.

167 randed DNA binding protein ICP8 and promotes strand exchange in vitro in conjunction with ICP8.

168 In any eukaryote, RAD51-directed strand exchange in vivo is mediated by further factors,

169 d activity of yeast and human Rad52: inverse strand exchange, in which Rad52 forms a complex with dsD

170 that Mph1 and Mus81-Mms4 recognize an early strand exchange intermediate and direct repair to noncro

171 and eukaryotic Rad51 and Dmc1 all stabilize strand exchange intermediates in precise three-nucleotid

172 The strand exchange intermediates that accumulate in ruv mut

173 iments, we demonstrate that the mechanism of strand-exchange involves active coupling of unwinding an

174 nts improves at pH 8.5, whereas complete DNA strand exchange is also restored.

175 DNA strand exchange is also slowed commensurate with the rat

176 nition of homologous sequences of DNA before strand exchange is considered to be the most puzzling st

177 The efficiency of strand exchange is highly sensitive to the location, typ

178 e presence of mismatches and where the first strand exchange is homology-independent.

179 How DNA mismatches affect Dmc1-mediated DNA strand exchange is not understood.

180 n filament disassembly and completion of DNA strand exchange is observed.

181 This mode of coordination of strand exchanges is unique among tyrosine recombinases.

182 Homologous pairing and strand exchange lead to the formation of DNA intermediat

183 cally to inhibit RecA during an on-going DNA strand exchange, likely through the disassembly of RecA

184 Thus, the changes in tension inherent to strand exchange may couple with ATP hydrolysis to increa

185 ns that are thought to be brought about by a strand exchange mechanism involving the N-terminal extra

186 the barrel, consistent with a proposed beta-strand exchange mechanism.

187 peats that polymerize into a pilus through a strand-exchange mechanism.

188 common and idiosyncratic features in the DNA strand exchange mechanisms of three RecA-family recombin

189 ggest they also directly promote the DNA-RNA strand exchange necessary for hybrid formation since we

190 Strand exchange nucleic acid circuitry can be used to tr

191 These sites flank the overlap regions where strand exchanges occur.

192 interactions with one another through donor strand exchange, occurring at the usher, in which the N-

193 ction in binding free energy, and subsequent strand exchange occurs in precise 3-nt steps, reflecting

194 dinally organized chromosome axes and stable strand exchange of crossover-designated DSBs.

195 gs and increases the free energy penalty for strand exchange of non-homologs.

196 nity, it has minimal effects on WRN-mediated strand exchange of telomeric DNA.

197 d1 protein complex stimulates Dmc1-catalyzed strand exchange on homologous DNA or containing a single

198 single strand annealing and ATP-independent strand exchange on short duplexes.

199 A junctions, cleaving 1nt 3' to the point of strand exchange on two strands symmetrically disposed ab

200 DNA strand exchange plays a central role in genetic recombin

201 ng yeast provide evidence that Rad52 inverse strand exchange plays an important role in RNA-templated

202 omponents from parting ways and initiate the strand exchange process.

203 lity in the base pairing in the heteroduplex strand exchange product could provide stringent recognit

204 efficient and stringent formation of stable strand exchange products and which is consistent with a

205 s to be incorporated in otherwise homologous strand exchange products even though sequences with less

206 f ATP hydrolysis even 75 bp sequence-matched strand exchange products remain quite reversible.

207 crease the reversibility of sequence matched strand exchange products with lengths up to approximatel

208 insight into how ATP hydrolysis destabilizes strand exchange products.

209 nd its cognate RecA led to inhibition of DNA strand exchange promoted by RecA.

210 plants, with sequence similarity to the RecA strand exchange protein and a role in homologous recombi

211 RAD51 DNA strand exchange protein catalyzes the central step in ho

212 ependent of RAD51, which encodes the central strand exchange protein in yeast required for conservati

213 In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop formation

214 The DNA strand exchange protein RAD51 facilitates the central st

215 led DNA, which serves as a substrate for the strand exchange protein Rad51.

216 iated stimulation of the other budding yeast strand exchange protein Rad51.

217 hat invokes fork regression catalyzed by the strand exchange protein RecA as an intermediate in the p

218 mulates the activity of the meiosis-specific strand exchange protein ScDmc1 only 3-fold, whereas anal

219 equires nucleases that resect DSB ends and a strand exchange protein that facilitates homology search

220 The meiosis-specific DNA strand exchange protein, DMC1, promotes the formation of

221 Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference for

222 A strand exchange protein, RAD51, is also required for rep

223 Here we report that the S. cerevisiae DNA strand exchange protein, Rad51, prevents Rad52-mediated

224 rhang, which becomes a substrate for the DNA strand exchange protein, Rad51.

225 and examined the activity of SsoSSB with the strand-exchange protein S. solfataricus RadA (SsoRadA).

226 AD51 and other members of the RecA family of strand exchange proteins assemble on ssDNA to form presy

227 th of these properties are common to all DNA strand exchange proteins examined thus far.

228 ssed ends are substrates for assembly of DNA strand exchange proteins that mediate DNA strand invasio

229 l domains that interact with and recruit DNA strand exchange proteins to DNA.

230 is of homologous chromosomes, persistence of strand exchange proteins, and alterations in both the fr

231 nd segregation failure require RecA and RecF strand exchange proteins.

232 RAD51 and DMC1 are the strand-exchange proteins forming a nucleofilament for st

233 defining member of a ubiquitous class of DNA strand-exchange proteins that are essential for homologo

234 After DNA strand exchange Rad51 protein is stuck on the double-str

235 ends of the complementary strand reduces the strand-exchange rate for homologous filaments.

236 ant protein is capable of catalyzing the DNA strand exchange reaction and is insensitive to inhibitio

237 00 to 200-fold, and promoting the subsequent strand exchange reaction approximately 10 to 20-fold.

238 cleoprotein filaments on DNA that catalyze a strand exchange reaction as part of homologous genetic r

239 nd Dmc1-catalyzed homologous DNA pairing and strand exchange reaction have also been identified.

240 that PcrA can inhibit the RecA-mediated DNA strand exchange reaction in vitro.

241 for limiting ssDNA; and (3) no formation of strand exchange reaction products.

242 al-time fluorescence with a toehold-mediated strand exchange reaction termed one-step strand displace

243 RecA's ability to promote LexA cleavage and strand exchange reaction, and are believed to modulate i

244 DNA annealing function to actively catalyze strand-exchange reaction between the unwinding substrate

245 Unlike strand-annealing, the strand-exchange reaction requires nucleotide hydrolysis

246 ge helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a s

247 Strand exchange reactions catalyzed by phosphorylated ve

248 and diagnostic applications, similar to how strand exchange reactions in solution have been used for

249 ismatched base pairs that impede uncatalyzed strand exchange reactions led to a significant decrease

250 nherent ability to promote DNA annealing and strand exchange reactions on free as well as RPA-coated

251 uman RAD51 protein catalyzes DNA pairing and strand exchange reactions that are central to homologous

252 bined biochemical reconstitutions of the DNA strand exchange reactions with total internal reflection

253 shape changing films that are powered by DNA strand exchange reactions with two different domains tha

254 to dif and carry out two pairs of sequential strand exchange reactions.

255 ification circuits based on toehold-mediated strand exchange reactions.

256 Its presence in DNA strand-exchange reactions in vitro results in a signific

257 UvsX and RecA catalyze similar strand-exchange reactions, but differ in other propertie

258 erization on DNA substrates and catalysis of strand-exchange reactions.

259 rically activated to catalyze ATPase and DNA strand-exchange reactions.

260 RAD51 is the central strand exchange recombinase in somatic homologous recomb

261 (NPF) that catalyzes homologous pairing and strand exchange (recombinase) between DNAs that ultimate

262 ons with arm-type sites dictate the order of strand exchange regardless of the orientation of the ove

263 tes (FRTs) harboring non-homology within the strand exchange region does not yield stable recombinant

264 The identification of the most common strand exchange regions of these 78 deletions served to

265 ctions is that the first step, XerD-mediated strand exchange, relies on interaction with the very C-t

266 and DNA (ssDNA) binding, ssDNA annealing and strand-exchange (SE) activities.

267 cation (LAMP), programmable toehold-mediated strand-exchange signal transduction, and standard pregna

268 Some mismatches next to the first strand exchange site (in reactions with C(-3)G attBT1-1

269 N and telomeric DNA, stimulates WRN-mediated strand exchange specifically between telomeric substrate

270 coli RecA protein catalyzes the central DNA strand-exchange step of homologous recombination, which

271 is to initiate the homology recognition and strand-exchange steps and those of hRad54 are to promote

272 ent to generate PiDSD, an intermolecular DNA strand-exchange strategy to measure a set of key kinetic

273 eus, BRCA2(BRCI-8) stimulates RAD51-mediated strand exchange, suggesting it may be an essential co-fa

274 anism: a sequence identity-dependent initial strand exchange that requires two base pairs of compleme

275 DNA strand exchange, the central step of homologous recombin

276 is a bystander during the annealing step of strand exchange, the enzyme strongly discriminates again

277 ent sequence specificity of toehold-mediated strand exchange, the OSD reporter could successfully dis

278 gh Cre and Int use the same mechanism of DNA strand exchange, their respective reaction pathways are

279 e that hRad54 can facilitate hRad51-promoted strand exchange through various degrees of mismatching.

280 tential mismatches and facilitate long-range strand exchanges through branch migration of Holliday ju

281 other 10FNIII domain via a Trp-mediated beta-strand exchange to stabilize a partially unfolded interm

282 ures exploits Watson-Crick hybridization and strand exchange to stitch linear duplexes into finite as

283 n using pre-steady-state kinetic analysis of strand exchange using oligonucleotide substrates contain

284 Strand exchange usually terminates after a single round

285 d the possibility that propagation solely by strand exchange was a significant contributor to transfe

286 of bacterial genomes; however, commitment to strand exchange was believed to occur after testing appr

287 the ability of RAD54 to stimulate RAD51 DNA strand exchange was not significantly affected by SN, in

288 Unlike Rad51, Brh2 was able to promote DNA strand exchange when preincubated with double-stranded D

289 e complementary strand significantly retards strand exchange, whereas applying the same force to the

290 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward

291 h core-type sites adjacent to the regions of strand exchange, while the N-terminal arm binding (N) do

292 tes long 3' single-strand tails that undergo strand exchange with a homologous chromosome to form joi

293 consequence, RecA-RFP is proficient for DNA strand exchange with dATP or at lower pH.

294 in nucleotide exchange, RPA displacement and strand exchange with full-length DNA substrates.

295 single-stranded DNA (ssDNA), which catalyzes strand exchange with homologous duplex DNA.

296 ad52 forms a complex with dsDNA and promotes strand exchange with homologous ssRNA or ssDNA.

297 recombinase-DNA covalent complex can undergo strand exchange with intact duplex dif in the absence of

298 ssing entails 5' end resection and preferred strand exchange with the homolog rather than the sister

299 emodeling the Rad51 filament, priming it for strand exchange with the template duplex.

300 unbinds, whereas homologous dsDNA undergoes strand exchange yielding heteroduplex dsDNA in site I an