ライフサイエンスコーパス: strand invasion

1 ingle-strand DNA substrate for RecA-mediated strand invasion.

2 from two homologs anneal as a consequence of strand invasion.

3 buted in nature: single-strand annealing and strand invasion.

4 tion (HR), where it mediates RAD51-dependent strand invasion.

5 ciate with homologous donor sequences during strand invasion.

6 for a homologous donor sequence and promotes strand invasion.

7 ctions, suggesting that Mnd1p is involved in strand invasion.

8 can make PNAs even more powerful agents for strand invasion.

9 an ideal substrate for RecA protein-promoted strand invasion.

10 hesize as little as 30 nucleotides following strand invasion.

11 relatively short hybrid regions, suggesting strand invasion.

12 rified HOP2 alone is proficient in promoting strand invasion.

13 ination, suggesting DNA end resection before strand invasion.

14 rified HOP2 alone is proficient in promoting strand invasion.

15 1/2 inhibitor-induced DNA lesions before DNA strand invasion.

16 nucleoprotein filaments that can mediate DNA strand invasion.

17 ed nucleosomes in the HML donor locus during strand invasion.

18 ns of recombination, homology search and DNA strand invasion.

19 DNA lesion bypass that is distinct from DNA strand invasion.

20 ilize long duplex donors that participate by strand invasion.

21 ase triplet steps during the early stages of strand invasion.

22 sults in a block to meiotic DSB repair after strand invasion.

23 responsible for key mechanistic steps during strand invasion.

24 ppresses ssDNA annealing and facilitates DNA strand invasion.

25 OH for all markers downstream of the site of strand invasion.

26 NA strand exchange proteins that mediate DNA strand invasion.

27 ble-stranded heteroduplex DNA product of DNA strand invasion.

28 mbination performing homology search and DNA strand invasion.

29 tein bound to the heteroduplex DNA after DNA strand invasion.

30 p2/Mnd1) that greatly enhances Dmc1-mediated strand invasion.

31 dges Rad51 from ssDNA preventing promiscuous strand invasions.

32 Red system can operate--strand annealing or strand invasion--accounts well for these findings.

33 point mutation in Hop2 that dissociates its strand invasion activity from its ability to bind and an

34 This strand invasion activity of ICP8 coupled with DNA synthe

35 ssDNA may explain its ability to enhance the strand invasion activity of the T4 recombinase (UvsX) an

36 tes to the DNA lesion bypass through its DNA strand invasion activity.

37 We provide the first report of strand-invasion activity in plant mitochondria.

38 ble-strand break (DSB) triggers HR repair by strand invasion also in BRCA2-defective cells, but less

39 e cells are not completely impaired in HR by strand invasion although the spontaneous HR rate is 10-f

40 t mobile D-loops that mimic structures in HR strand invasion and at telomere ends.

41 ater step in homologous recombination, after strand invasion and before the initiation of new DNA syn

42 ologous recombination protein, necessary for strand invasion and crossing over.

43 way was considerably less active than single-strand invasion and did not contribute significantly to

44 single-stranded DNA (ssDNA), preventing DNA strand invasion and exchange by homologous recombination

45 asion intermediates in a manner that favours strand invasion and exchange.

46 Mre11 bound to partly processed DSBs impairs strand invasion and HR.

47 Partner choice is determined at the time of strand invasion and is mediated by two recombinases: Rad

48 delays Rad51p binding to ssDNA and prevents strand invasion and localization of Rad51p to HML alpha.

49 dmc1Delta mutants are strongly defective in strand invasion and meiotic progression and that these d

50 nduced in the palindrome promotes homologous strand invasion and repair synthesis, similar to mitotic

51 Error-free, conservative HR involves strand invasion and requires a homologous DNA template,

52 range homology search that occurs before the strand invasion and transfer reactions.

53 Dimeric bis-PNAs capable of both strand invasion and triplex formation can form clamp str

54 combinants (by single-strand annealing or by strand invasion) and to convert recombinational intermed

55 The repair did not involve Rad51-driven strand invasion, and moreover the suppression of strand

56 Rates of strand invasion are temperature dependent and can be enh

57 nt background and was consistent with single-strand invasion/assimilation without mismatch correction

58 s a ssDNA-dependent ATPase that can catalyze strand invasion at both saturating and subsaturating con

59 sothermal nucleic acid amplification method, Strand Invasion-Based Amplification (SIBA), which exhibi

60 ctions that help ensure Dmc1-mediated stable strand invasion between homologous chromosomes, thereby

61 eveals that rad52-R70A cells can mediate DNA strand invasion but are unable to complete the recombina

62 Pol delta complex mutants are proficient for strand invasion but unable to complete extensive tracts

63 BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is

64 t of the designed peptide increases rates of strand invasion by 100-fold relative to unmodified bisPN

65 e, 3' tails must presumably be generated for strand invasion by 5'-3' exonuclease activity.

66 c recombination mediator that stimulates DNA strand invasion by both Dmc1 and Rad51.

67 t not the individual proteins, can stimulate strand invasion by Dmc1.

68 To increase the versatility of strand invasion by PNAs, we have synthesized bisPNAs and

69 ditions should further expand the utility of strand invasion by PNAs.

70 ein onto the DSB ends at MAT, the subsequent strand invasion by the Rad51 nucleoprotein filament into

71 We propose a model in which lower-stem strand invasion by Uc.283+A impairs microprocessor recog

72 ds inside bacteria clearly demonstrates that strand invasion can take place in a biologically relevan

73 sequence targeted by the PNA is the shortest strand invasion complex reported to date.

74 Irradiation of the strand invasion complex results in asymmetric cleavage o

75 uch that the C-terminal T5sequence forms the strand invasion complex, leaving the N-terminal T5sequen

76 ugate has been synthesized and shown to form strand invasion complexes with a duplex DNA target.

77 argets in a sequence-specific manner forming strand-invasion complexes.

78 p) is the product of homology search and DNA strand invasion, constituting a central intermediate in

79 RAD51-independent recombination by one-ended strand invasion coupled to DNA synthesis, followed by si

80 l for DSB-induced gene conversion in which a strand invasion creates a modified replication fork, inv

81 n concert with Rad51 and Dmc1 to promote the strand invasion (D-loop formation) step of homologous re

82 or gaps in nonsister homologous chromatids), strand invasion disparity, and different correction freq

83 show that BIR can occur by several rounds of strand invasion, DNA synthesis and dissociation.

84 ccumulating evidence for Rad51-catalyzed DNA strand invasion during double-strand break repair featur

85 the length of hDNA formed by the initiating strand invasion event determines susceptibility of the r

86 a homologous donor sequence and a subsequent strand invasion event on chromatin fibers.

87 Strand invasion events mature into double Holliday junct

88 ant JM structures that result from secondary strand-invasion events and often require Mus81-Mms4 for

89 defect observed for rad51 mutants is due to strand invasion failure, whereas the Pol delta complex m

90 to a model where joining is initiated by 3' strand invasion followed by pairing to short repeat sequ

91 Mismatch correction of strand invasion heteroduplex DNA is strongly polar, favo

92 change proteins forming a nucleofilament for strand invasion, however, the function of the paralogues

93 Here we show that ICP8 also catalyzes strand invasion in an ATP-independent manner.

94 meiotic recombination to promote homologous strand invasion in the budding yeast Saccharomyces cerev

95 demonstrate that oligonucleotides promoting strand invasion in the DNA double helix can significantl

96 t study shows that beta protein can initiate strand invasion in vitro, as evidenced both by the forma

97 nd invasion, and moreover the suppression of strand invasion increased repair with oligonucleotides.

98 ws for the formation of the first detectable strand invasion intermediate (i.e., single-end invasion)

99 for BIR and gene conversion suggest a common strand invasion intermediate in these two recombinationa

100 ults show that WRN clearly prefers to act on strand invasion intermediates in a manner that favours s

101 nction is critical for stabilizing analogous strand invasion intermediates that exist in two separate

102 suggest a failure to disassemble RAD-51 from strand invasion intermediates.

103 ly one free end and are thought to repair by strand invasion into a homologous duplex DNA followed by

104 th only one end available for repair undergo strand invasion into a homologous duplex DNA, followed b

105 this amplification: a facilitation of primer strand invasion into double-stranded DNA, and a suppress

106 nsive semiconservative DNA replication after strand invasion into homologous template DNA.

107 er chromatid is repaired via RAD51-dependent strand invasion into the regenerated sister.

108 DNA synthesis after Rad51-mediated DNA strand invasion is a crucial step during recombination.

109 The ability to catalyze strand invasion is a novel activity of ICP8 and the firs

110 ed a system using purified proteins in which strand invasion is coupled with DNA synthesis.

111 Strand invasion is most efficient when pyrimidine PNAs a

112 results can be explained by a model in which strand-invasion leading to gene conversion competes more

113 he structural results, TRF2 DBD stimulated a strand invasion-like reaction, associated with telomeric

114 rmation of a t-loop which is stabilized by a strand invasion-like reaction.

115 capable of promoting recombination by a DNA strand invasion mechanism.

116 SDSA requires strand invasion mediated by DmRad51, the product of the

117 In accord with a strand-invasion mode of complex formation, the pcPNA bin

118 e partially Mus81 dependent, suggesting that strand invasion occurs and the stalled intermediate is s

119 Strand invasion occurs in a highly sequence-specific man

120 After that, the strand invasion occurs via Watson-Crick pairing between

121 n single-stranded DNA, Rad52 likely promotes strand invasion of a double-stranded DNA molecule by sin

122 Aside from promoting Rad51-mediated DNA strand invasion of a partner chromatid, Rad54 and Rdh54

123 Strand invasion of different templates by both DSB ends

124 romosomes and that most replication involves strand invasion of internal regions by the ends of linea

125 mation, and (2) it suppresses Rad51-mediated strand invasion of sister chromatids via a Rad54-indepen

126 by coupling unwinding of U4/U6 stem II with strand invasion of stem I.

127 TRF2 forms the T-loops by stimulating strand invasion of the 3' overhang into duplex DNA.

128 mbination at telomeres, leading to increased strand invasion of the 3' overhang within t-loop junctio

129 the cleaved recipient leading to homologous strand invasion of the donor allele, the assay was perfo

130 ay due to loss of a MEK1 stimulated bias for strand invasion of the homologous chromosome.

131 for evidence that Mek1 positively stimulates strand invasion of the homologue.

132 tly and energetically facilitates the direct strand invasion offered by collapse at positions +1 to +

133 In contrast, Rad51, which mediates strand invasion, only associates with DSBs that relocali

134 e immediate vicinity of the break was not by strand invasion or strand annealing pathways.

135 s that engage homologous sequences either by strand invasion or strand annealing.

136 can be directed to either Rad51-mediated DNA strand invasion or to Rad52-mediated DNA annealing.

137 Rapid strand invasion over a wide range of experimental condit

138 These results confirm that the single-strand invasion pathway of homologous recombination is t

139 ppear to be repaired primarily by the single-strand invasion pathway of homologous recombination.

140 Thus, either the strand invasion pathway(s) in which BRCA2 operates is st

141 22 appears to function outside of the single-strand invasion pathway, but levels of etoposide-induced

142 hat are central to both strand annealing and strand invasion pathways of recombination.

143 prepares the invading telomeric overhang for strand invasion, possibly through end processing or thro

144 rporates the well-established RecA-catalyzed strand invasion process with a novel stabilizing hybridi

145 opagated throughout the oligonucleotide by a strand invasion process.

146 The displacement loop (D loop) is a DNA strand invasion product formed during homologous recombi

147 g subsequent Rad54p-dependent formation of a strand invasion product.

148 protein, DMC1, promotes the formation of DNA strand invasion products (joint molecules) between homol

149 Hence, strand invasion products formed by ICP8 are resistant to

150 ncrease the persistence of SsoRadA catalyzed strand invasion products.

151 in spn-A, which encodes the ortholog of the strand invasion protein Rad51.

152 ecA family of recombinases catalyzes the DNA strand invasion reaction that takes place during homolog

153 ganisms employ RecA orthologues to guide the strand invasion reactions necessary for DNA recombinatio

154 roteins that catalyze homologous pairing and strand-invasion reactions.

155 ologous DNA sequences and the subsequent DNA strand invasion required to initiate HR.

156 es our results were consistent with separate strand invasion/resolution at the two ends of the target

157 Simple strategies for enhancing strand invasion should facilitate the use of PNAs: (i) a

158 SSA competes with interhomologue strand invasion significantly more successfully when Mek

159 on structure that is thought to exist at the strand invasion site of the t-loop.

160 osal that crossover interference acts at the strand invasion stage of recombination.

161 up a defined in vitro system for the initial strand invasion step of double-strand break repair.

162 Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required follow

163 loops (D-loops), which represent the initial strand invasion step of HR.

164 ing event that occurs in vivo during the DNA strand invasion step of HR.

165 hat mimics an intermediate formed during the strand invasion step of many recombinational processes.

166 nce that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DN

167 hows no intrinsic polarity preference at the strand invasion step that initiates double-strand break

168 These pathways share common features in the strand invasion steps, but differ in subsequent repair s

169 ys a central role in the homology search and strand invasion steps, DSBs either are not repaired or a

170 requency, or there is a separate pathway for strand invasion still functional in BRCA2-deficient cell

171 binding would also allow TRF2 to stabilize a strand invasion structure that is thought to exist at th

172 ion DNA, and on a substrate that resembles a strand invasion structure.

173 facilitates the recognition of duplex DNA by strand invasion, suggesting that antigene PNAs (agPNAs)

174 talled forks, suggesting that RAD51-mediated strand invasion supports fork restart.

175 ouble-strand gap requires multiple cycles of strand invasion, synthesis, and dissociation of the nasc

176 tion requires much less homology (30 bp) for strand invasion than does RAD51-dependent repair (approx

177 These results suggest a minor pathway of strand invasion that is dependent on RAD52 but not on RA

178 be mechanistically distinct from the initial strand invasions that establish BIR.

179 esults and on the ability of TRF2 to mediate strand invasion, that TRF2 plays an essential role in HR

180 tors that load the Rad51 filament to promote strand invasion, the defining feature of HR.

181 tly to clear Rad51 from DNA after successful strand invasion, thereby enabling the downstream events

182 this we show that in vitro Rad22 can promote strand invasion to form a D-loop that can be cleaved by

183 nctions to mediate repair via homologous DNA strand invasion to form D-loops.

184 rt a model in which DmBlm acts downstream of strand invasion to unwind a D-loop intermediate to free

185 onucleotide conjugates initiate and maintain strand invasion under more stringent conditions than do

186 on permits the Rad51 proteins to promote DNA strand invasion using either 3'- or 5'-ends with similar

187 tein may regulate the choice between the DNA strand invasion versus annealing pathways.

188 to the RecA-type recombinases that catalyze strand invasion via an active search for homology, ICP8

189 Strand invasion was efficient at neutral to basic pH, a

190 onal regulatory role for Rad51 following DNA strand invasion, where Rad51-double-stranded DNA may inh

191 through the resolution of an intratelomeric strand invasion which resembles a t-loop.

192 on single-stranded DNA (ssDNA) and catalyzes strand invasion with homologous duplex DNA.

193 chromatid repair, as distinct from enforcing strand invasion with the homologue.

194 on either 3' or 5'-ssDNA tails and promotes strand invasion without regard for the polarity of the t