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1 des that mimic key interactions of biotin to streptavidin.
2 rmation, and panned against the model target streptavidin.
3 form a complex that is held onto immobilized streptavidin.
4 nding to cis-divalent but not trans-divalent streptavidin.
5 n was immobilized for binding of Cy5-labeled streptavidin.
6 tinylated rhodium piano stool complex within streptavidin.
7 using gold nanoparticles (AuNPs), biotin and streptavidin.
8 rties, and strong interaction of biotin with streptavidin.
9 s M13 bacteriophage high selectivity for the streptavidin.
10 h low and high dissociation constant: biotin/streptavidin (10 fM) and HER2/HER2 antibody (0.44 +/- 0.
11 method to stepwise evolve peptide binders to streptavidin, a protein studied for over two decades and
12 incubated with increasing concentrations of streptavidin, achieving a limit of detection (LOD) of 5n
14 scribe a hybridization assay based on biotin-streptavidin affinity using lanthanide-labeled reporter
15 of the labeled extracts, trypsin digestion, streptavidin-affinity purification of peptides containin
20 oxyphenyl-functionalized SPCEs modified with streptavidin and a sandwich type immunoassay was impleme
21 es to pretargeting, strategies predicated on streptavidin and biotin, bispecific antibodies, compleme
22 a 2 x 2 junction array, functionalized with streptavidin and biotinylated antibodies specific for Es
23 onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinyla
27 wo different ligand-receptor systems (biotin/streptavidin and TNF-alpha) over a wide range of concent
28 age libraries to the N- or C-termini of core streptavidin and used them to setup phage-free noncompet
29 DNA anchors we attached a spherical protein (streptavidin) and a rod-shaped DNA (47bp) to a quartz cr
31 ed by the covalent attachment of antibodies, streptavidin, and oligonucleotides and the binding and b
32 quantified using phycoerythrin (PE)-labeled streptavidin, and the fluorescence was analyzed in a Lum
34 of labeled biomolecules such as antibodies, streptavidin, and tubulin proteins and showed that stabl
35 tionalized by coating with polyethyleneimine-streptavidin-anti-Escherichia coli antibodies to improve
36 roscopy (MA/SERS), which utilizes a Fe3O4@Ag@streptavidin@anti-IgG nanocomposite with strong magnetic
39 e oxidase, DNA/hydrogen peroxide, and biotin/streptavidin, are measured by the paper-based micro-calo
40 and analogues, with their strong binding to streptavidin, are used in many clinical laboratory tests
41 for doing this involved using membrane-bound streptavidin as a biomarker for the charge on the membra
42 a new desthiobiotin surface, using wild-type streptavidin as a robust bridge between the chip and the
44 A towards a small peptide substrate carrying streptavidin at its distal end was also investigated to
45 We also show accumulation of model analyte (streptavidin at nanomolar concentration in nanoliter eff
46 The limiting step at present is binding of streptavidin at the end of DNA to biotin on capture bead
47 res of native-like cations of serum albumin, streptavidin, avidin, and alcohol dehydrogenase were pro
48 in-protein systems: biotin-avidin and biotin-streptavidin, barstar-dibarnase and Z domain-immunoglobu
50 re optionally sheared, affinity-purified via streptavidin bead immobilization, and subjected to tradi
51 and biotinylated proteins are enriched with streptavidin beads and identified by mass spectrometry.
53 magnitude higher than previous results using streptavidin beads and the limit of detection (LOD) impr
54 2-aminoethyl methanethiosulfonate-biotin and streptavidin beads to determine their aqueous-accessibil
56 A complexes are then labeled with an enzyme (streptavidin-beta-galactosidase), and single enzymes ass
58 labeled streptavidin is used to quantify the streptavidin binding capacity of each mesh type through
59 lent streptavidins enabled us to uncover how streptavidin binding depends on the nature of the biotin
61 body-epithelial cell surface-binding, biotin-streptavidin binding, and the topologically enhanced cel
62 plemental biotin ingestion may affect biotin-streptavidin binding, leading to potential clinical misi
63 within or immediately after a 38-amino acid streptavidin-binding peptide (SBP) that is appended to t
64 ld-type AR tagged at its N terminus with the streptavidin-binding peptide epitope (streptavidin-bindi
65 says using truncated kinesin-1 motors with a streptavidin-binding peptide tag that can attach to stre
66 th the streptavidin-binding peptide epitope (streptavidin-binding peptide-tagged wild-type androgen r
67 ted to the highly specific bacterial protein streptavidin binds to biotinylated macromolecules like p
70 conjugated via biotinylated glycan (through streptavidin-biotin affinity) followed by linkage of hem
77 bioreceptor layer was formed using a common streptavidin-biotin immobilization strategy and employed
78 and QD, and the high binding affinity of the streptavidin-biotin interaction, we achieved multiplexed
80 ntibody, or streptavidin: the high-stability streptavidin-biotin linkage improved sensitivity by an o
81 oated substrates and a strong but reversible streptavidin-biotin linkage to PEG-coated AFM tips enhan
84 nal RIT (directly radiolabeled antibody) and streptavidin-biotin pretargeted RIT (PRIT) directed agai
85 in the limit-of-detection, the inclusion of streptavidin-biotin simplifies the development of simila
86 43, 75, and 292 for the IgG-antibody assay, streptavidin-biotin system, and covalently attached BSA,
88 we found that integrin receptors dissociate streptavidin-biotin tethered ligands in focal adhesions
95 ayer surface (SAMs) was used for immobilized streptavidin-biotinylated probes on the sensor surface f
96 also solved the structure of trans-divalent streptavidin bound to biotin-4-fluorescein, showing how
98 mate focusing position and separation of the streptavidin-bound biotin, anti-biotin-bound biotin, and
103 ) the classical assay that works with biotin-streptavidin chemistry, (II) the rapid assay that is per
105 first demonstration that recombinant peptide-streptavidin chimeras can be used for sensitive immunode
107 ctionalized (4FB) magnetic beads rather than streptavidin coated beads with a high density of capture
108 ement of optical read-out was obtained using streptavidin coated gold-nanoparticles interacting with
109 ary anti-S.aureus aptamer was immobilized on streptavidin coated magnetic beads (MB), which serves as
110 rotocol based on biotinylated DNA probes and streptavidin coated magnetic beads we were able to selec
111 nt of a 384-well immuno-PCR method that uses streptavidin coated on a PCR plate to capture complexes
112 ich assay, which employed nanoEnhancers (NIR-streptavidin coated quantum dots) for ultrasensitive det
113 rum with a fully automated platform based on streptavidin coated tips and a biotinylated mouse anti-h
115 n the ECM through bioconjugation between the streptavidin-coated beads and the collagen fibers and th
117 usly captured bacteria, and (III) binding of streptavidin-coated gold nanoparticles to the biotinylat
118 lated anti-human EGFR Apt was immobilized on streptavidin-coated magnetic beads (MB) and served as a
119 apture of biotinylated ligation junctions on streptavidin-coated magnetic beads and PCR amplification
120 lution and thus were further integrated into streptavidin-coated magnetic beads for purification of V
121 drug and catabolites were released from the streptavidin-coated magnetic beads, separated by monolit
122 the warhead, 8-base PNA strands, biotin, and streptavidin-coated magnetic beads, we demonstrate retri
124 nd the two antibodies is then captured using streptavidin-coated magnetic microparticles and directly
125 with a biotinylated secondary antibodies and streptavidin-coated MB resulted in a limits of detection
126 rase reaction products, which immobilized on streptavidin-coated microplate, hybridized with biotinyl
128 the basic sandwich type assay, performed in streptavidin-coated microtitration wells, the limit of d
129 on binding, the complex was harvested on the streptavidin-coated microwell, and subsequently the form
130 Stx binding to Pk analogues immobilized on streptavidin-coated plates was assessed by enzyme-linked
131 ling nanofiber meshes outperform traditional streptavidin-coated polystyrene plates under flow, valid
132 rategy that is enabled by the combination of streptavidin-coated quantum dot (QD) acceptors and bioti
133 the oriented immobilization of antibodies to streptavidin-coated surfaces, such that the antigen bind
134 d with the human adenosine deaminase (hADA1)-streptavidin complex and adenosine as a detection system
138 uding 23 assays that incorporated biotin and streptavidin components and 14 assays that did not inclu
139 nd 14 assays that did not include biotin and streptavidin components and served as negative controls.
140 against matched targets (carbonic anhydrase, streptavidin, concanavalin A) to identify desired ligand
141 beta-lactoglobulin and enolase), tetrameric (streptavidin, concanavalin A, and pyruvate kinase), and
142 tection from 10 nmol L(-1) to 320 pmol L(-1) streptavidin concentration with a much higher sensitivit
144 otinylated detection antibodies, fluorescent streptavidin conjugate, and wash buffer for a total volu
145 Furthermore, optical signal enhancement with streptavidin conjugated quantum dots was shown to yield
149 s using biotinylated antibodies labeled with streptavidin-conjugated Pb- and Cd-based quantum dots (Q
153 geometry of the lipid layers, nucleation of streptavidin crystals occurred specifically on the DPN-p
154 By labelling a DNA replication fork with streptavidin (dsDNA end) and Fab (5' ssDNA) we located t
156 ate the focusing behavior of Dylight labeled streptavidin (Dyl-Strep) proteins in the channel, using
157 R-Phycoerythrin (RPE) and Dylight labeled streptavidin (Dyl-Strep) were focused within a nanochann
158 mplexed with a biotinylated aptamer bound to streptavidin efficiently increased the SPR signal by com
160 ion that capitalizes on the high affinity of streptavidin for biotin but does not require dissociatio
162 ed a more powerful way to isolate monovalent streptavidin, for ultra-stable labeling without undesire
163 mine rates of DNA unwinding, displacement of streptavidin from biotinylated DNA, translocation on sin
164 h biotin to form a noncovalent link with the streptavidin functionalized AFM tip during the approach
165 linkers (BLs) to mediate the aggregation of streptavidin-functionalized gold nanoparticles (st-AuNPs
166 ffinity and the chemical characterization of streptavidin-functionalized gold nanoparticles (STV-NPs)
167 ed oligonucleotides) and (ii) the capture of streptavidin-functionalized gold nanoparticles to the af
169 d lateral flow assay was based on the use of streptavidin gold nanoparticles for the labelling of the
171 ) the biotinylated detection antibody, (iii) streptavidin horseradish peroxidase, (iv) a wash buffer,
172 was then revealed by the introduction of the streptavidin-horseradish peroxidase conjugate that catal
174 er labeling the hybridized biotin-miRNA with streptavidin-HRP conjugates, amperometric detection at -
176 n-labeled terminal of the probe bound to the streptavidin immobilized on the lateral flow test strip,
177 ect the presence of less than a femtomole of streptavidin in 10 muL of sample using fluorescence imag
179 e-labeled amplicons, and fluorophore-labeled streptavidin in order to study the functionality and dis
182 ersible decoration of DNA origami tiles with streptavidin, including revealing an encrypted Morse cod
183 ti-IL-17A catch antibodies, which via biotin-streptavidin interaction are bound to the biotinylated s
184 t a method based on the high affinity biotin-streptavidin interaction that allows selective capture o
189 limit of detection for anti-DNP antibody and streptavidin is 1.2x10(-10) g/ml (0.55 pM) and 2.3x10(-1
193 t of the biotin-tagged tryptic peptides with streptavidin; (iv) liquid chromatography-tandem MS (LC-M
199 ed and detected by the GMR sensor by linking streptavidin magnetic nanoparticles (MNPs) to the sensor
200 nvolving a sandwich hybridization assay onto streptavidin-magnetic beads (Strep-MBs), hybridization c
203 ated cells, DNA end-labeling with biotin and streptavidin-mediated chemiluminescent detection of the
204 wide variety of molecules including dextran, streptavidin, microspheres, and lentivirus particles.
207 d on the glassy carbon electrode (GCE) using streptavidin modified-gold nanoparticles/thiolated graph
208 enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles (MNP@Strep)
210 , and finally allows the immobilization of a streptavidin-modified ruthenium-based ECL label via reac
213 th a central scaffold protein linked to four streptavidin molecules, each having three pMHC ligands o
215 zol-4-yl)(biotin-cap-NBD-PE) lipids and that streptavidin more effectively outcompeted the anti-bioti
216 relying on fluorophore-conjugated monomeric streptavidin (mSA) to label membrane proteins carrying a
217 how that cross-linking of biotinylated HA in streptavidin multimers or supramolecular complexes with
218 biosensing of thrombin with the use of gold-streptavidin nanoparticles (strep-AuNPs) and silver redu
219 held even when the DNA duplex is tethered to streptavidin or the mobility of the nitroxides is altere
220 e demonstrated the quantitative detection of streptavidin over a broad range of concentrations down t
221 his work, using the well-acknowledged biotin-streptavidin pair as a benchmark, we go forward in the d
222 oteins cholera toxin B subunit-Alexa 647 and streptavidin-PE/Cy5, to membranes containing different a
227 alized plasmonic AuNSs substrate but against streptavidin previously conjugated to gold nanorods, the
228 detect 526 ymol of the alkaline phosphatase streptavidin probe and accurately quantify zeptomole amo
230 (SAM) were designed and applied for binding streptavidin, promoting affinity biosensing and enzyme a
231 ct Transistor (FBI-OFET) sensor, embedding a streptavidin protein capturing layer, capable of perform
232 ollowing PVP removal, biotinylated thiol and streptavidin protein were added to the nanostars, which
235 y remain nearly constant upon varying the Ir/streptavidin ratio [up to 78% ee (S)-salsolidine, kcat 2
236 on of S112 reveals a marked effect of the Ir/streptavidin ratio on both the saturation kinetics as we
237 mg/mL and temperature changes in biotin and streptavidin reaction are presented to demonstrate the f
238 e (LSPR) shift-based biosensors using biotin-streptavidin recognition interaction as a proof-of-conce
241 -divalent streptavidins retained tetravalent streptavidin's high thermostability and low off-rate.
243 advance is made possible by incorporating a streptavidin (SA) hydrogel capture/purification element
247 amples of blank ImmunoCAPs coupled to either streptavidin (SA-CAP-1 or 2) or nonallergenic maltose-bi
248 oiety (Cp* = C5Me5(-)) within homotetrameric streptavidin (Sav) (referred to as Cp*Ir(Biot-p-L)Cl] su
250 strate that a cysteine-containing variant of streptavidin (Sav) can serve as a protein host to model
251 otinylated iridium pianostool complex within streptavidin (Sav) isoforms was selected as a model reac
252 by developing a sequence-independent biotin-streptavidin (SAv) roadblocking strategy that simplifies
256 which self-assembles on gold film, afforded streptavidin sensing with surface plasmon resonance (SPR
258 iotinylated liposomes to surface-immobilized streptavidin show that the refractive index of the encap
262 ecific cell captures using a micro-patterned streptavidin (STR)-functionalized silicon nanowire (SiNW
265 e we report the solution of a selenobiotinyl-streptavidin structure using phases obtained by the anom
271 we present an efficient approach to isolate streptavidin tetramers with two biotin-binding sites in
272 ptors (EGFR) using biotinylated EGF bound to streptavidin that is covalently coupled in an ordered ar
273 beads with Protein L, secondary antibody, or streptavidin: the high-stability streptavidin-biotin lin
275 olved the crystal structures of cis-divalent streptavidin to 1.4A resolution and trans-divalent strep
276 avidin to 1.4A resolution and trans-divalent streptavidin to 1.6A resolution, validating the isolatio
277 sive activated SA-aptamers could capture the streptavidin to achieve enhancement and output of the de
282 ation of three tetrameric protein complexes (streptavidin, transthyretin, and hemoglobin) in the gas
283 phycoerythrin-biotin (PhycoE-Biotin) and Cy5-streptavidin trapped in the two proteoliposome populatio
288 n detecting a high molecular weight analyte (streptavidin) versus in the flow-over PSi approach.
290 on (LOD) of the MGNT arrays for detection of streptavidin was estimated as 25nM, with a detection tim
292 atch crystallization screening setups, where streptavidin was used as a model protein for crystalliza
294 ffinities of biotin and desthiobiotin toward streptavidin, we demonstrate selective and reversible de
296 In our experiment, microbeads coated with streptavidin were driven to the sensors in the center of
297 rations (4.17pM to 41.7nM) of dye-conjugated streptavidin were simultaneously infused through the sec
298 tinylated substrate that, when captured with streptavidin, were resistant to challenge with competito
299 lymers with the ability to specifically bind streptavidin while minimizing the nonspecific binding of
300 y and 4,7-dihydroxy-1,10-phenanthroline into streptavidin yields an NAD(P)H-dependent artificial tran
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