ライフサイエンスコーパス: streptolysin

1 o Schwann-like STS26T cells permeabilized by streptolysin 0.

2 ts of PEG on zymosan, lipopolysaccharide, or streptolysin-induced inflammatory and bioenergetic respo

3 Streptolysin-induced necrosis of human neutrophils was r

4 ith CLI reduced extracellular DNase Sda1 and streptolysin O (SLO) activity in vivo, whereas subinhibi

5 articipate in CMT, the pore-forming molecule streptolysin O (SLO) and an effector protein with the ch

6 Two such proteins, streptolysin O (SLO) and NAD(+)-glycohydrolase (NADase),

7 Two such products, streptolysin O (SLO) and NAD+-glycohydrolase, appear to

8 Although the toxicity of streptolysin O (SLO) and streptolysin S (SLS) in purifie

9 NADase (SPN) and streptolysin O (SLO) are two toxins that play important

10 tudies identified the pore-forming cytolysin streptolysin O (SLO) as necessary and sufficient for the

11 including the SpeB cysteine protease and the streptolysin O (SLO) cytolysin, but not SIC, a protein t

12 rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes whic

13 ssumes that the NAD-glycohydrolase (nga) and streptolysin O (slo) genes that code for these products

14 Streptolysin O (SLO) is a cholesterol-dependent cytolysi

15 cell lines using cationic lipid systems and streptolysin O (SLO) is used to effect their delivery.

16 duction of the cholesterol-binding cytotoxin streptolysin O (SLO) prevented internalization of GAS in

17 ed the abundance of streptolysin S (SLS) and streptolysin O (SLO) production between clinically domin

18 er permeabilization with the bacterial toxin streptolysin O (SLO) requires endocytosis via a novel pa

19 utilizes the cholesterol-dependent cytolysin Streptolysin O (SLO) to translocate the NAD(+) -glycohyd

20 utilizes the cholesterol-dependent cytolysin Streptolysin O (SLO) to translocate the NAD(+) glycohydr

21 athogen Streptococcus pyogenes that utilizes streptolysin O (SLO), a cholesterol-dependent cytolysin.

22 rulence factor that is present among most is streptolysin O (Slo), a protein with well-characterized

23 f the archetypical member of the CDC family, streptolysin O (SLO), a virulence factor from Streptococ

24 bronectin-binding proteins (sfbI and fbp54), streptolysin O (slo), hyaluronic acid capsule (hasA), st

25 rol-binding CDCs, perfringolysin O (PFO) and streptolysin O (SLO), were found to exhibit strikingly d

26 easuring the properties of Ca2+ signaling in streptolysin O (SLO)-permeabilized cells were used to st

27 ion of the secreted pore-forming haemolysin, streptolysin O (SLO).

28 Ca2+ signalling after permeabilization with streptolysin O (SLO).

29 haride and/or large amounts of the cytotoxin streptolysin O (SLO).

30 eted cytotoxins S. pyogenes NADase (SPN) and streptolysin O (SLO).

31 ular toxins NAD+-glycohydrolase (NADase) and streptolysin O (SLO).

32 g the immunity factor IFS and the cytolysin (streptolysin O [SLO]), were more abundant in the mutant

33 ne response to SCPA correlated with the anti-streptolysin O and anti-DNase B responses.

34 lored various methods including TAT peptide, Streptolysin O and microporation for delivering NeutrAvi

35 Streptolysin O damaged and killed the cells quickly, all

36 ion of toxic shock syndrome toxin-1, whereas streptolysin O directly damages the mucosa to allow for

37 e pores are produced by perfringolysin O and streptolysin O during insertion (and not small pores tha

38 alpha-toxin and the streptococcal cytolysin streptolysin O enhanced penetration of toxic shock syndr

39 While reports have linked sloR function to streptolysin O expression, transport experiments with ra

40 Permeabilization with streptolysin O for 10 minutes permitted the loss of free

41 A similar analysis conducted on streptolysin O from Streptococcus pyogenes revealed that

42 alpha-hemolysin from Staphylococcus aureus, streptolysin O from Streptococcus pyogenes, and anthroly

43 When delivered with streptolysin O into living human epithelial cancer cells

44 ected host cell via the pore-forming protein streptolysin O is unknown.

45 were introduced to HL-60 cells by use of the streptolysin O pore-forming peptide.

46 by TLR2 and TLR4 ligands in the presence of streptolysin O required Myd88/Trif, whereas that induced

47 Streptolysin O treatment of infected human macrophages i

48 was similar to mga, i.e., slo (which encodes streptolysin O) and plr (encoding the plasmin receptor/g

49 or) but did not affect transcription of slo (streptolysin O), mga (multiple gene regulator of GAS), e

50 between patient groups with nonfilarial Ag (streptolysin O)-stimulated supernatant (LP = 0.160 relat

51 mmalian cells has been achieved by employing Streptolysin O, a bacterial enzyme which forms temporary

52 hils, that this activity was attributable to streptolysin O, and that platelet/neutrophil complex for

53 by collagenase digestion, permeablized with streptolysin O, and the release of Ca2+ from internal st

54 requires NF-kappaB and the virulence factor streptolysin O, but proceeds independently of P2X7R and

55 tion of antibodies to HlpA and antibodies to streptolysin O, indicating that the histone-like protein

56 In MIN6 beta-cells permeabilized by streptolysin O, insulin release was stimulated by elevat

57 omosomal region encoding secreted NADase and streptolysin O, is the key driver of increased toxin pro

58 e genes encoding streptokinase, CAMP factor, streptolysin O, M protein (more abundant in the CvfA(-)

59 n of other extracellular products, including streptolysin O, streptokinase, and DNase, was not affect

60 A when delivered into P815 target cells with streptolysin O, whereas transfection of target cells wit

61 Examples include the pore-forming cytotoxin streptolysin O, which oligomerises to form large pores i

62 t NAD-glycohydrolase is translocated through streptolysin O-generated pores into a host cell.

63 in or addition of Ca2+-containing buffers to streptolysin O-permeabilized cells induced exocytosis of

64 um) of isolated microsomes, Ca2+ uptake into streptolysin O-permeabilized cells, and analysis of SERC

65 In streptolysin O-permeabilized cells, guanosine 5'-O- (2-t

66 t vastly different transport capabilities in streptolysin O-permeabilized cells, in accordance with t

67 secretion when introduced after docking into streptolysin O-permeabilized cells; so does a Rab3A-22A

68 pendent manner, both in vitro and in vivo in streptolysin O-permeabilized chromaffin cells.

69 bility of inducers to elicit exocytosis when streptolysin O-permeabilized human sperm were loaded wit

70 y of SCAMP2, potently inhibits exocytosis in streptolysin O-permeabilized mast cells.

71 dent fashion, as shown using both intact and streptolysin O-permeabilized parasites.

72 tron microscopy and proteolytic digestion of streptolysin O-permeabilized parasitized erythrocytes.

73 Using a streptolysin O-permeabilized platelet exocytosis assay,

74 d in alpha-granule secretion, we developed a streptolysin O-permeabilized platelet model of alpha-gra

75 Streptolysin O-permeabilized platelets released alpha-gr

76 Streptolysin O-permeabilized platelets synthesized PtdIn

77 Exposure of streptolysin O-permeabilized platelets to antihuman cell

78 Incubation of streptolysin O-permeabilized platelets with an antibody

79 Incubation of streptolysin O-permeabilized platelets with phosphatidyl

80 ed Ca2+-induced alpha-granule secretion from streptolysin O-permeabilized platelets.

81 -2, rVAMP-3, and rVAMP-8 were incubated with streptolysin O-permeabilized platelets.

82 In streptolysin O-permeabilized PMNs, PLD was directly acti

83 Streptolysin O-permeable pancreatic acini were used to s

84 ed wild-type amounts of type 3 M protein and streptolysin O.

85 e Dynasore also protected HeLa cells against streptolysin O.

86 ly permeabilised with the pore-forming toxin streptolysin O.

87 od of plasma membrane permeabilization using streptolysin O.

88 ng secreted toxins NAD(+)-glycohydrolase and streptolysin O.

89 i) reversible membrane permeabilization with streptolysin O.

90 tory effect of an unrelated cytolytic toxin, streptolysin O.

91 equired expression of the pore-forming toxin streptolysin O.

92 ing enhanced by the effects of M protein and streptolysin O.

93 sed for the homologous member of the family, streptolysin O.

94 ludes listeriolysin O, perfringolysin O, and streptolysin O.

95 ided that cells are first permeabilized with Streptolysin-O (SL-O), and (2) chimeric methylphosphonod

96 using either cells gently permeabilized with streptolysin-O (SLO) or microsomes from homogenized cell

97 her streptococcal cell wall antigen (SCW) or streptolysin-O (SLO) to augment lung injury in rats.

98 In streptolysin-O (SLO)-perforated rat brain cortical synap

99 dies recognizing specific domains of apoB in streptolysin-O (STP-O)- and saponin-permeabilized HepG2

100 kidney (PK-15) cells were permeabilized with streptolysin-O and incubated with a library of 125I-labe

101 and permeabilization of cultured acini with streptolysin-O and insertion of GDP beta S or antibodies

102 Permeabilization by streptolysin-O and introduction of guanosine thiodiphosp

103 ization of plasma membranes by detergents or streptolysin-O in hepatocytes, thymocytes, and P19, Jurk

104 Mast cells permeabilized with streptolysin-O leak soluble (cytosol) proteins over a pe

105 Streptolysin-O permeabilization was used to introduce an

106 phate via the recording patch pipette or via streptolysin-O permeabilization.

107 an antagonist of Gq/11, was introduced into streptolysin-O permeabilized acini to bypass the plasma

108 When streptolysin-O permeabilized CHO cells were incubated wi

109 concentration ([Ca2+]i) from both intact and streptolysin-O permeabilized isolated nerve endings of t

110 sopressin (AVP) secretion in both intact and streptolysin-O permeabilized nerve endings.

111 We have developed a novel assay system using Streptolysin-O permeabilized neutrophils that recapitula

112 ify this, we measured resting Ca2+ sparks in streptolysin-O permeabilized ventricular myocytes from w

113 Anti-streptolysin-O titres were elevated in 65% of patients.

114 were used in cultured acini permeabilized by streptolysin-O to determine the role of the G proteins i

115 ced into the cells via permeabilization with streptolysin-O, and cellular uptake was confirmed by flu

116 efect was preserved by permeabilization with streptolysin-O, it was determined that Lec35p is not dir

117 Streptolysin-O-mediated guanosine 5'-3-O-(thio)triphosph

118 ant CRHSP-28 (rCRHSP-28) was introduced into streptolysin-O-permeabilized acinar cells, and amylase s

119 late Ca(2+)-stimulated secretory activity in streptolysin-O-permeabilized acinar cells.

120 In streptolysin-O-permeabilized acini of transgenic mice, a

121 2+) uptake into the endoplasmic reticulum of streptolysin-O-permeabilized cells by sarco/endoplasmic

122 tion of function-blocking Sec8 antibodies to streptolysin-O-permeabilized cells revealed exocyst requ

123 tentiated InsP(3)-evoked Ca(2+) release from streptolysin-O-permeabilized cells.

124 with the slow leak rate of the protein from streptolysin-O-permeabilized cells.

125 ing in vitro also inhibits acid secretion in streptolysin-O-permeabilized gastric glands.

126 In streptolysin-O-permeabilized MDCK cells, Sec8 antibodies

127 the presence of the ionophore A23187 and in streptolysin-O-permeabilized semi-intact cells.

128 y permeabilized with the pore-forming toxin, streptolysin-O.

129 CvfA(-) mutant), SpeB, mitogenic factor, and streptolysin S (less abundant).

130 ated hasABC operon, streptokinase (ska), and streptolysin S (sagA) during growth in the presence of 1

131 Increased transcript levels of sagA and streptolysin S (SLS) activity during exponential-phase g

132 acid capsule synthesis and exotoxins, e.g., streptolysin S (SLS) and pyrogenic exotoxin B (SpeB).

133 ranscription of sagA, a gene associated with streptolysin S (SLS) and speB, the gene encoding pyrogen

134 We also compared the abundance of streptolysin S (SLS) and streptolysin O (SLO) production

135 et-activating factor acetylhydrolase Sse and streptolysin S (SLS) have synergistic contributions to i

136 ugh the toxicity of streptolysin O (SLO) and streptolysin S (SLS) in purified group A streptococci (G

137 Streptolysin S (SLS) is the cytolytic factor that create

138 The oxygen-stable hemolysin streptolysin S (SLS) of Streptococcus pyogenes is encode

139 Streptolysin S (SLS) produces the hallmark beta-haemolyt

140 Notably, the entire sag operon necessary for streptolysin S (SLS) production was under CcpA-mediated

141 ually all group A streptococci (GAS) produce streptolysin S (SLS), a cytolytic toxin that is responsi

142 nt phosphotransferase system (PTS) exhibited Streptolysin S (SLS)-mediated hemolysis during exponenti

143 ccus sag operon encoding the beta-haemolysin streptolysin S (SLS).

144 factors produced by GAS is the peptide toxin streptolysin S (SLS).

145 es a powerful cytolytic bacteriocin known as streptolysin S (SLS).

146 s secretes a highly cytolytic toxin known as streptolysin S (SLS).

147 ficient in expression of the cytolytic toxin streptolysin S (SLS-) and evaluated events that occur du

148 e demonstrate the in vitro reconstitution of streptolysin S activity.

149 lting strain, JRS470, produced no detectable streptolysin S and showed a drastic reduction in cell su

150 resulted from enhanced transcription of the streptolysin S biogenesis operon.

151 a genetic locus immediately upstream of the streptolysin S biosynthetic operon in several GAS genome

152 Although it has been widely held that streptolysin S exerts its lytic activity through membran

153 For the streptolysin S hemolysin, a dramatic increase in hemolyt

154 sing high-resolution live cell imaging, that streptolysin S induces a dramatic osmotic change in red

155 ption of ska (encoding streptokinase), sagA (streptolysin S), and speMF (mitogenic factor) but did no

156 ag promoter, which directs the expression of streptolysin S, a hemolysin that can damage the membrane

157 hat resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Stre

158 The amounts of streptokinase, streptolysin S, and capsule paralleled the levels of tra

159 ificantly reduced the haemolytic activity of streptolysin S, and dramatically reduced the pathology i

160 (capsule, cysteine protease, streptokinase, streptolysin S, and streptodornase).

161 s necessary and sufficient for production of streptolysin S, another GAS virulence factor, is also ne

162 n unrelated second streptococcal haemolysin, streptolysin S, during infection of keratinocytes.

163 otypes: decreased beta-hemolysis mediated by streptolysin S, reduction in the activity of a secreted

164 ergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is

165 s provide key insights into the mechanism of streptolysin S-mediated haemolysis and have implications

166 ion of a small peptide toxin by GAS known as streptolysin S.

167 ence factors, including the potent cytolysin streptolysin S.

168 to the promoter region of the gene encoding streptolysin S.

169 cular insight into the chemical structure of streptolysin S.

170 The expression of the streptolysin toxins is strongly upregulated, whereas gen

171 during adherence to host cells, GAS delivers streptolysin toxins, creating endoplasmic reticulum stre