ライフサイエンスコーパス: stringency

1 der identical assay conditions (no change in stringency).

2 ctively, to HIF-1alpha or HIF-2alpha at high stringency.

3 ies, however, are not uniform and often lack stringency.

4 g both proper timing and one round per cycle stringency.

5 ore remotely related proteins with a reduced stringency.

6 dize with the leaf form in RNA blots at high stringency.

7 related deaths by 29-71%, depending on their stringency.

8 for analyses requiring very high analytical stringency.

9 cal suggestions for the control of selection stringency.

10 ates and microorganisms, even under very low stringency.

11 I were sufficient to impart this selectivity/stringency.

12 III fragment that was detectable at only low stringency.

13 nds at low stringency and rare bands at high stringency.

14 s in both catalytic efficiency and substrate stringency.

15 tified 1,757 phosphorylation sites with high stringency.

16 uch as photonically controlled hybridization stringency.

17 requirement for MeCP2 markedly increases in stringency.

18 triphosphates incorporation with sufficient stringency.

19 applicable for the problems that demand high stringency.

20 oter melting capacity for increased promoter stringency.

21 uality-based trimming across a wide range of stringencies.

22 tion of telomerase at different purification stringencies.

23 Several high stringency 14-3-3 binding sites that overlapped with Akt

24 ng 3 rounds of phage selection of increasing stringency 6 unique scFvs that bound purified TSP by enz

25 At high stringency, 70%-90% of all annotated genes are detected

26 the investigator to decide on the filtering stringency according to desired data variability, and ou

27 re, we report the identification of 337 high-stringency allele-specific H3K4me3 and H3K36me3 peaks in

28 Using high-stringency analysis criteria (changes of twofold or grea

29 llenged with CRPV at low, moderate, and high stringencies and monitored for papilloma formation.

30 e of the salt results in increased substrate stringency and dependence on the full GroE system for re

31 The stringency and evolutionary forces governing between-hos

32 choice of mapper and variant caller, mapping stringency and filtering of SNPs by read mapping quality

33 lleviated by altering denaturation/digestion stringency and indicates why protein calibrators may not

34 The high stringency and low sample volumes of NECEEM, although ge

35 ere were usually a few CA or SU bands at low stringency and rare bands at high stringency.

36 here ground on this work and illustrate the stringency and successful general applicability of our a

37 important but incomplete description of the stringency and thermodynamic origin of metal-ion selecti

38 massively parallel pipeline to achieve high stringency and throughput.

39 th of cells, their responses to stresses and stringencies, and the virulence of pathogens.

40 s and regulatory targets, tunable regulatory stringency, and rapid response to input availability.

41 including assembling contigs using variable stringencies, assembling contigs using fingerprints from

42 SAR parallels between the two series lost stringency at distal positions.

43 Reduced stringency at this checkpoint may obstruct the B cell re

44 ding an elastic system that varies selective stringency based on homeostatic demands.

45 also suggest that increasing climate policy stringency beyond a certain degree may lead to diminishi

46 ture-specific probes do not require the high stringency binding conditions necessary for methods base

47 of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate

48 e UniGene gene index was screened using high stringency BLAST against a pool of endothelial expressed

49 could be amplified under various degrees of stringency by PCR or hybridized by Southern blotting fro

50 Here we report that this stringency can be altered by the nature of different pro

51 holds of phylogenetic conservation and motif stringency can be altered dynamically to facilitate deta

52 he objective of the screening procedure, its stringency can be modified by altering the level of expr

53 ove the prediction accuracy; increasing rule stringency can improve predictions and reduce extraneous

54 o a situation where a slow rise in selection stringency caused by a changing environment leads to dir

55 single-stranded DNA under both low and high stringency conditions (0.5 M NaCl).

56 t support primer extension/elongation at low stringency conditions during PCR sample preparation when

57 f proteins co-immunoprecipitating under high stringency conditions from peak Xenopus ENaC/Apx-contain

58 of Rpa1 bound single-stranded DNA under low stringency conditions only (0.1 M NaCl), while a central

59 Ikaros and Helios associated under moderate stringency conditions only with other members of the Ika

60 Southern blot analysis under high-stringency conditions showed that strains of all 12 sero

61 n blot with a p2y3 receptor probe, under low-stringency conditions that allowed the clear identificat

62 a melanogaster genomic library using reduced stringency conditions to identify closely related genes.

63 otic organisms were performed under moderate stringency conditions with a Syrian hamster-specific cph

64 PCR (under high-stringency conditions) with primers for the mur-2(ed) ge

65 However, under low stringency conditions, both tagged proteins assembled in

66 bridised to the rye 1RS chromatin under high stringency conditions, indicating the presence of shared

67 ction digestion and hybridization under high-stringency conditions, the epa gene cluster of OG1RF (wh

68 Under reduced-stringency conditions, three additional proteins bind to

69 y Southern hybridization analyses under high-stringency conditions, we found that DNA fragments conta

70 this method include probe concentration and stringency conditions.

71 s, B. pilosicoli, or B. murdochii under high-stringency conditions.

72 ized with those isolates only under very-low-stringency conditions.

73 an additional member was detected under low-stringency conditions.

74 fragment containing the cap1 genes under low-stringency conditions.

75 bioengineered without c-MYC achieve highest stringency criteria for bona fide cardiogenesis enabling

76 Using high stringency criteria to define in vitro vertebrate topois

77 ifferences persist when we use (1) different stringency cutoffs, (2) different selection inference mo

78 single TFF (Trefoil factor) domains as high stringency determinants.

79 Low-stringency DNA blot analysis indicated that F3H is encod

80 PCR and high-stringency DNA hybridization confirmed mutagenesis and t

81 High stringency DNA hybridization confirmed the presence of t

82 to involve increased translation initiation stringency during stress-induced eIF2alpha-P, we observe

83 a nonoptimal Region 2.3 to increase promoter stringency, enabling them to mount a focused response to

84 And hybridization at low stringency ensures that all targets will be preferably h

85 ng and that nucleation site stability is the stringency factor that prevents in vivo polymerization.

86 hat different dynein functions have distinct stringencies for motor performance.

87 sis generation and imposes considerably more stringency for accuracy in identifying informative gene

88 rm cross-beta-sheet structures of sufficient stringency for incorporation into the amyloid fibril.

89 rast, IS1630 appears to exhibit an increased stringency for inverted repeat recognition, since the ma

90 istration of exogenous IFN-alpha relaxes the stringency for selection in the germinal centers resulti

91 -B27-peptide complexes might have rather low stringency for the peptide sequences.

92 Mapping stringency for type assignment was determined based on 8

93 Low-stringency genomic sequence analysis of targeted regions

94 Low stringency genomic Southern blot analysis indicates that

95 Generation of a hybridization stringency gradient allows perfect-matched hybrids to be

96 The stringency gradient is achieved by carrying out the hybr

97 ngements with similarity at a high degree of stringency (>99%) across the otherwise unrelated and div

98 n control rabbits (at the moderate challenge stringency) had a 0% probability of remaining disease fr

99 Here we report the isolation, by low stringency homology cloning from a hypothalamic library,

100 G2, a strain that is, as determined by high-stringency hybridization analysis, genetically distinct

101 High-stringency hybridization detected only one MDR-like gene

102 Low-stringency hybridization in a Southern analysis reveals

103 We used low-stringency hybridization of a chicken embryonic cDNA lib

104 (DBD) has previously depended mainly on low stringency hybridization of cDNA libraries and degenerat

105 orebrain and its microvasculature using high stringency hybridization of poly(A)+ RNA isolated from t

106 similar (sima) gene was isolated using a low-stringency hybridization screen employing a Dm single-mi

107 /EBP alpha, beta, gamma, and delta using low-stringency hybridization screening and computer searches

108 the chicken homologue was isolated using low-stringency hybridization screening of a T-lymphocyte cDN

109 nuclear genome as single-copy genes, but low-stringency hybridization suggested the presence of a mul

110 We have used low stringency hybridization to clone a novel connexin from

111 lled CaNIK1 identified in C. albicans by low stringency hybridization using CaSLN1 as a probe.

112 merase chain reaction amplification, and low stringency hybridization we have identified six novel fr

113 70-kDa S6 kinase (S6K) were isolated by low-stringency hybridization with mammalian p70S6k probes.

114 Through relaxed-stringency hybridization, we obtained the cDNA encoding

115 se organisms by sequence comparisons and low-stringency hybridization.

116 obe, the human homologue was obtained by low stringency hybridization.

117 Low- as well as high-stringency hybridizations detected only a single rpoN ge

118 Increased statistical stringency identified 158 and 252 unique PVAN and TCMR i

119 genes as probes under conditions of moderate stringency identified sequences homologous to these gene

120 High stringencies identify a core set of highly conserved gen

121 Moreover, data from low stringency immunoprecipitation experiments suggest that

122 Using a low stringency immunoprecipitation of human FcRn, we observe

123 og of FcRn is endowed with unexpectedly high stringency in binding specificity for IgGs.

124 COG technique was modified so that variable stringency in COG construction was possible.

125 iple parameter sets with different levels of stringency in computation of an optimal alignment of two

126 tumor latency, suggesting a need for greater stringency in dietary assignment for transgenic mouse mo

127 Curiously, the Q91N mutant exhibited stringency in discriminating between correct and incorre

128 d during evolution and is detectable at high stringency in invertebrates.

129 brary by systematically tuning the selection stringency in screening.

130 rived peptide mass spectra require increased stringency in their interpretation.

131 nic hybridization of the target DNA, at high stringency, in <5 min.

132 sed to generate call sets of varying quality stringency, including consensus calls present across all

133 articipants' sex, years of practice, and the stringency index (within-group z score of initial 3 rati

134 ondition (good vs poor performances) and the stringency index jointly accounted for 45% of the observ

135 20% among benchmark sequences at the lowest stringency, indicating that many genes in public databas

136 High stringency is critical to avoid slippage of hybrids alon

137 Sequence stringency is imparted by specific Hoogsteen hydrogen bo

138 This overall stringency is relaxed in the 11 S regulator (PA26)-20 S

139 What remain unknown are when and with what stringency Lck is required for effective TCR-mediated ac

140 d second version of miRDeep suggest that the stringency level of each of these programs may, in fact,

141 Different stringency levels are likely to affect the number of pos

142 comparisons suggest that, despite differing stringency levels they all identify a similar set of kno

143 d a combined caller across the full range of stringency levels, which outperforms all of the individu

144 ion of reporter gene expression at different stringency levels.

145 ilies, called tribes, using three clustering stringencies (low, medium and high).

146 lysis using the Pfam server identified a low stringency match to a fatty acid desaturase domain in th

147 The increased stringency may be explained by a mild temperature sensit

148 chicken CD30 promoter has 15 predicted high-stringency Meq-binding transcription factor recognition

149 aded bioinformatic searches we devised a low stringency method for isolating proteins from an Arabido

150 High stringency Northern blotting revealed a tissue-specific

151 miRBase annotations and identifying at high stringency novel miRNAs and their targets.

152 ify the incidence of ERE sequences under two stringencies of detection: <10% and 10-20% nucleotide de

153 re: self-MHC and self-peptide diversity, the stringencies of positive and negative selection, and the

154 es on MS2 VLPs and that allow control of the stringency of affinity selection through the manipulatio

155 The stringency of ARC-seq can be scaled to accommodate the q

156 This may be useful for altering the stringency of binding enrichment during phage display.

157 It is important to elucidate the stringency of bottlenecks during transmission to shed li

158 It is possible that the lower stringency of CD8(+) T cell activation obviates a requir

159 es-specific determinant which influences the stringency of cellular response to microtubule depolymer

160 e pole morphology, chromosome alignment, and stringency of checkpoint signaling, resulting in error-p

161 uncondensed chromatin, and to determine the stringency of chromatin binding.

162 demand-side efficiency, and changes with the stringency of climate targets.

163 A measure of transmissibility and of the stringency of control measures required to stop an epide

164 quires additional regulators to increase the stringency of control, and as will be discussed, to gain

165 Since then, the stringency of criteria for HLA matching have been libera

166 differing clinical outcomes improve with the stringency of criteria, underscoring the need for compar

167 User defined inputs specify the stringency of data filtering and the method and conditio

168 ase-activated state and thereby decrease the stringency of decoding.

169 This has resulted in decreased stringency of diagnostic criteria, which in turn has res

170 Stringency of dietary control varied from addition of su

171 g that FOXF1 is required for maintaining the stringency of DNA replication and genomic stability.

172 he K65R substitution appears to increase the stringency of dNTP binding.

173 end on insecticide development over time and stringency of environmental regulation.

174 Adjusting the stringency of hybridization enabled single-base mismatch

175 hybridization based nucleic acid sensors the stringency of hybridization poses a challenge to design

176 Confounding factors may relate to stringency of inflammatory disease diagnosis and the eff

177 ing on the start codon, thereby altering the stringency of initiation.

178 been extensively characterized in vitro, the stringency of its search and sensitivity to mismatched s

179 yeast (Saccharomyces cerevisiae) include the stringency of metabolic control at the level of oxygen c

180 Such stringency of migration most likely imposes an immense c

181 ynonymous codon usage affects the efficiency/stringency of mRNA decoding, mRNA biogenesis/stability,

182 although BAFF/APRIL inhibition increases the stringency of negative selection of the naive autoreacti

183 This order, combined with the stringency of negative selection, is shown to lead to ef

184 We quantify the stringency of our methodology and relate it to previous

185 celerated SMG-1 kinase activity and enhanced stringency of phosphorylation site selection.

186 its output that allows the user to vary the stringency of predictions.

187 nctions of sigmas is critical to setting the stringency of promoter recognition.

188 e multiplicity to enhance the efficiency and stringency of regulation.

189 we show targets COPII protein to reduce the stringency of retention.

190 s indicate that DNA hairpins can enhance the stringency of RNase H targeted degradation of the RNA si

191 The trait allele frequencies, the stringency of sample ascertainment, the number of marker

192 ese high frequencies appear to be due to the stringency of selection with promoterless constructs, be

193 el can be inferred directly from data on the stringency of selection, and that, in agreement with rec

194 increase in error rates was affected by the stringency of selection, the trait allele frequency, and

195 se-containing media was used to increase the stringency of selection.

196 7 of HBV core antigen (HBcAg), lost the high stringency of selectivity in genome maturity during viri

197 ogical studies and a concomitant increase in stringency of significance thresholds.

198 elationship between intron abundance and the stringency of splice-site requirements; (iii) the tenden

199 The stringency of start codon selection impacts the efficien

200 e codons in preferred context to examine the stringency of start codon selection in the model filamen

201 ound that many P-site mutations increase the stringency of start codon selection.

202 disruption of bridges B3 and B7b alters the stringency of start codon selection.

203 NA(i)(Met), but have opposite effects on the stringency of start codon selection: the CTT increases,

204 Two metrics of stringency of stomatal control of psi, (1) a 'hydroscape

205 vs. anisohydry has been used to describe the stringency of stomatal regulation of plant water potenti

206 ated new and previously developed metrics of stringency of stomatal regulation of psi during soil dry

207 ydrolysis to increase the directionality and stringency of strand exchange.

208 tions instead of Mg(2+) often diminishes the stringency of substrate selection and incorporation fide

209 strain is proposed to result from a relaxed stringency of termination by RF1 at the stop codon (UAG)

210 As the DNA length and stringency of the binding reaction increase, greater pre

211 Finally, the stringency of the checkpoint response was enhanced in mu

212 ing a catalytic structure are related to the stringency of the chemistry catalyzed and define a funct

213 n by other C/EBP family proteins and the low stringency of the consensus sequence.

214 eling may vary dramatically depending on the stringency of the criteria for selecting evidence to use

215 To investigate the stringency of the Escherichia coli selenocysteine insert

216 e selectable to allow the user to decide the stringency of the homologies.

217 chromosomes a significant relaxation in the stringency of the methylated state of the paternal allel

218 Given the high stringency of the model it is remarkable that we were ab

219 lack of response to univalents: (1) reduced stringency of the oocyte SAC to aberrant chromosome beha

220 or four rounds of selection by tailoring the stringency of the panning conditions.

221 The data also highlight the stringency of the Polycomb regulatory network, and show

222 that ebs1 mutations significantly reduce the stringency of the retention-based ER quality control, al

223 r IRhsp-1 and, at the same time, reduced the stringency of the spacer length requirement, with IRper-

224 l outcomes were monotonically related to the stringency of the viral challenge.

225 egulated throughout life, and nowhere is the stringency of this requirement more evident than during

226 ocyte stimulator (BLyS/BAFF) may enhance the stringency of TR-->FO selection by increasing TR B cell

227 LF1 transcription may therefore modulate the stringency of viral latency.

228 thought to play a key role in regulating the stringency of viral latency.

229 prise the 28 TIGR Gene Indices, we used high-stringency pair-wise sequence searches and a reflexive,

230 Additionally, we have used a low-stringency PCR cloning technique to identify ligands, re

231 A low stringency polymerase chain reaction (PCR) homology scre

232 Using the techniques of relaxed stringency polymerase chain reaction and genomic library

233 Using a low stringency polymerase chain reaction cloning method, we

234 phylogenetic domains was combined with high-stringency protein secondary structure prediction.

235 NOD FACTOR PERCEPTION encodes a putative low-stringency receptor that is responsible for calcium spik

236 -LIKE KINASE3 (LYK3) encodes a putative high-stringency receptor that mediates bacterial infection.

237 The increased stringency required for genome-wide analyses (with per-S

238 Increased selection stringency resulted in a distinct D-peptide inhibitor te

239 elatively rigid pocket would provide greater stringency, resulting in higher fidelity of DNA synthesi

240 y with radiolabeled chicken P2Y3 cDNA at low stringency revealed a cDNA clone encoding a novel G prot

241 Varying COG stringency reveals differences in the degree of conserva

242 ds were evaluated by exposure to a series of stringency rinses in solutions of successively lower ion

243 The signature set of 29 'high stringency' RIPD genes identified here were enriched for

244 hybridization to be carried out at very low stringency (room temperature), enabling a highly selecti

245 High stringency screening of a human aortic cDNA library for

246 Low stringency screening of a human kidney cDNA library and

247 -like homeobox genes were cloned through low-stringency screening of Arabidopsis cDNA libraries with

248 a second generation of mutagenesis and high-stringency screening, we identify several significantly

249 lso identified a gene related to Mstn by low-stringency screening.

250 unctional mutants in each library using high-stringency selection (>19,000-fold), the x factor was fo

251 t Saccharomyces cerevisiae under the highest stringency selection conditions, and PKA phosphorylated

252 amase with its protein inhibitor BLIP by low-stringency selection of a random TEM1 library using yeas

253 High stringency sequence comparison (e < 1 x10(-25)) of 157 g

254 luding oligonucleotide length, hybridization stringency, sequence identity, sample abundance, and sam

255 sembled according to user-defined filter and stringency settings.

256 We used a high-stringency similarity search by using BLASTN, and a prot

257 irrhinum AG orthologue, PLENA (PLE), and low-stringency Southern analysis demonstrated the absence of

258 Low-stringency Southern analysis suggests several additional

259 High-stringency Southern blots indicate that all five pathoge

260 ed to any walleye cellular gene based on low-stringency Southern blotting.

261 show the effects of an increasing selection stringency strategy and how it results in a tradeoff bet

262 e use of comparative sequence analysis, high-stringency structure prediction, proteolytic susceptibil

263 such that hybridization did not occur (high stringency), suggesting that the presence of the cationi

264 4 and/or 2 kb mRNA expression, even at high stringency (t(m)-8.8 deg. C).

265 d to bioinformatics calculation and P values stringency tests.

266 1) that Qa-2 are peptide receptors of higher stringency than ordinary class I molecules, and 2) that

267 med to a greater extent at a slightly higher stringency than the corresponding mismatched hybrids.

268 sual combination of high avidity and relaxed stringency that RI displays.

269 ivation (enabling ready control of selection stringency), the droplet size can be contracted (to conc

270 ous constraints that explain this mutational stringency, the structure does reveal a hydrophobic pock

271 of discrete RNA molecules hybridize at high stringency to cloned probes prepared from the 28S or 18S

272 e prohead RNA (pRNA) imparts specificity and stringency to the motor by ensuring the correct orientat

273 show that domain II imparts specificity and stringency to the motor during the packaging initiation

274 rains contain a gene that hybridizes at high stringency to the S. dysenteriae type 1 haem receptor ge

275 At lower stringency, up to 97% of annotated genes were identified

276 g a human fetal brain cDNA library under low stringency using cDNA encoding the mouse ligand of Cek5

277 ffinity clones under conditions of identical stringency using fluorescence-activated cell sorting.

278 This lack of stringency was compared with that of a peptide-dependent

279 wed by an overnight hybridization, short low stringency wash and detection of the labeled probe.

280 ted to achieve selectivity without a thermal-stringency wash.

281 Following low-stringency washes and a 2-day exposure, strongly hybridi

282 After stringency washes, the entire long arm of chromosome 6 (

283 ze the effect to the posthybridization array stringency washes.

284 n with genomic DNA followed by low- and high-stringency washes.

285 itions for optimal in situ hybridization and stringency washing, the detection of probes and the capt

286 To enhance stringency, we employed RNA silencing alone and together

287 screen a human brain cDNA library under low stringency, we have cloned a homologous cDNA preferentia

288 finding algorithms motif-x and MMFPh at high stringency, we identified compartmentalization of phosph

289 n 200 patterns of length 2 or greater at 99% stringency were found among the 72 genes we compared.

290 Using a high-stringency Western blotting protocol, the anti-1b antibo

291 r similar kinases could influence checkpoint stringency, which may differ among species and between s

292 uence differences is also critical: too much stringency will prevent recombination between different

293 ut B chromosomes by screening DNA at reduced stringency with a B centromeric probe.

294 eened a normal rat liver cDNA library at low stringency with a MDR1 cDNA fragment containing the P-lo

295 nd models by associating alignments of lower stringency with high-quality alignments.

296 A zoo-blot probed at moderate stringency with labeled cDNA revealed a strong conservat

297 Hybridization at low posthybridizational stringency with restriction enzyme-digested human genomi

298 Sequences hybridizing at high stringency with the P. syringae pv. tomato hrpW were fou

299 oute significantly influences the bottleneck stringency, with aerosol transmission imposing greater s

300 We used a high-stringency yeast two-hybrid method to screen a human hea