ライフサイエンスコーパス: structure determination

1 Geometry Problem (DGP) associated to protein structure determination.

2 llization is a rate-limiting step in protein structure determination.

3 ysics-based, Bayesian framework for improved structure determination.

4 has not been previously utilized for surface structure determination.

5 ut it is still insufficient for explicit ion structure determination.

6 n for single-crystal X-ray-diffraction-based structure determination.

7 on and the molecular mechanisms of cell wall structure determination.

8 ted previous attempts at crystallization and structure determination.

9 rotein crystals and electron diffraction for structure determination.

10 omain is the only one that has so far eluded structure determination.

11 que that allows for chemical state and local structure determination.

12 s possessing sufficient long-range order for structure determination.

13 emonstrates the potential of this method for structure determination.

14 g its overexpression in Escherichia coli and structure determination.

15 he potentials of this technology for cryo-EM structure determination.

16 y insoluble proteins for crystallography and structure determination.

17 e of the membrane mimetics commonly used for structure determination.

18 ority of folds that are easily accessible to structure determination.

19 uld be of value in both manual and automated structure determination.

20 be used as structural restraints in protein structure determination.

21 emains a major challenge in membrane protein structure determination.

22 d-state NMR with a powerful tool for de novo structure determination.

23 ng with those developed for crystallographic structure determination.

24 ligands may promote aaRS crystallization and structure determination.

25 ender them inappropriate for high-resolution structure determination.

26 ion between TNKS1 and USP25 by X-ray crystal structure determination.

27 s, resulting in accurate, unbiased, and fast structure determination.

28 ystallography is the main method for protein structure determination.

29 e predominant method used for macromolecular structure determination.

30 l-diffracting crystals that are suitable for structure determination.

31 ics of proteins--they have not been used for structure determination.

32 patterns, according to single-crystal X-ray structure determination.

33 -chain resonance assignment and unsupervised structure determination.

34 ues suitable for in meso crystallization and structure determination.

35 conformer is not visible in lower resolution structure determinations.

36 established by proton NMR and several X-ray structure determinations.

37 lysis, knowledge-base population and protein structure determination all benefit from human input.

38 collection strategy is suitable for routine structure determination and can be implemented at most m

39 regularity and flexibility make experimental structure determination and computational modeling chall

40 The combination of computationally assisted structure determination and experimental data analysis o

41 As shown by structure determination and in vitro measurements, this

42 s of proteins never observed by experimental structure determination and inaccessible to homology mod

43 d outline a strategy for the high-resolution structure determination and positioning of isotope-label

44 This approach enables direct structure determination and quantitative dynamics measur

45 e computational methods for crystallographic structure determination and structure prediction and opt

46 osphoproteins and demonstrate phosphoprotein structure determination and synthetic protein kinase act

47 cable and has direct implications in protein structure determination and the design of ligands or dru

48 High-resolution structure determination and thermodynamic analysis of fo

49 now be used to design constructs for protein structure determination and to develop enzyme inhibitors

50 tro kinetic binding, three-dimensional x-ray structure determination, and cell biology studies on the

51 Herein we report the synthesis, X-ray structure determination, and conformational analysis of

52 icant advancement in synthetic chemistry, in structure determination, and in the understanding of fac

53 toward this goal with the synthesis, crystal structure determination, and measurement of the molecula

54 inding studies with peptide mutagenesis, NMR structure determination, and molecular modeling, we have

55 he use of EM maps to help with X-ray crystal structure determination, as described in this protocol,

56 ardly benefit from the rapid pace of protein structure determination, as most newly solved structures

57 complexity, however, present a challenge for structure determination at atomic resolution.

58 X-ray crystallography allows macromolecular structure determination at both X-ray free electron lase

59 idly emerging as a powerful tool for protein structure determination at high resolution.

60 FX) in lipidic cubic phase (LCP) for protein structure determination at X-ray free-electron lasers (X

61 llization of CYP46A1 with racemic BIC led to structure determinations at 2.1 A resolution with the dr

62 Breakthroughs in G protein-coupled receptor structure determination based on crystallography have be

63 Methods of protein structure determination based on NMR chemical shifts are

64 ment, such information is rarely used in NMR structure determination because it can be incorrect, bot

65 The RV-C have been refractory to structure determination because they are difficult to pr

66 esidue two in our CTD construct used for NMR structure determination, but not present in the crystall

67 l boost in successful macromolecular complex structure determination by both X-ray crystallography an

68 Secondary structure determination by circular dichroism spectrosco

69 d GC-MS then jointly guided purification and structure determination by classical NMR methods.

70 Structure determination by cryo-electron microscopy has

71 led to this breakthrough in high-resolution structure determination by cryo-EM and point to challeng

72 The recent success in ribosome structure determination by cryoEM has opened the door to

73 sul crystallization of membrane proteins for structure determination by crystallographic approaches.

74 ectron spectrum, and the previously reported structure determination by electron diffraction.

75 ous structures plays a central role in X-ray structure determination by molecular replacement, such i

76 The method facilitates structure determination by NMR spectroscopy as exemplifi

77 Structure determination by NMR spectroscopy showed that

78 ns in proteins is an essential part of their structure determination by NMR spectroscopy.

79 one web server as a component of integrative structure determination by programs IMP, Chimera, and BI

80 Protein structure determination by proton-detected magic-angle s

81 y" approach has been demonstrated for atomic structure determination by serial X-ray diffraction of m

82 Three-dimensional (3D) structure determination by single-particle analysis of c

83 fferent steps and considerations involved in structure determination by single-particle cryo-EM to pr

84 cromolecular crystals that allows successful structure determination by single-wavelength anomalous d

85 rystals are thousands of times too small for structure determination by synchrotron X-ray diffraction

86 an provide a simple path for high-resolution structure determination by the cryoEM method MicroED and

87 y addressing one of the major bottlenecks to structure determination by X-ray crystallographic method

88 Structure determination by X-ray crystallography as well

89 to triage the most promising ligands for 3D structure determination by X-ray crystallography.

90 obtaining high quality protein crystals for structure determination by X-ray crystallography.

91 nknown structures, especially in cases where structure determination by X-ray single-crystal analysis

92 So far, all protein structure determinations carried out using FELs have bee

93 tegy to enhance the success rates of Fabs as structure determination chaperones.

94 challenges associated with membrane-protein structure determination, combines orientation restraints

95 d at the Advanced Photon Source) and ex situ structure determination confirmed the stability and pred

96 It is therefore clear that OBP structure determination constitutes the bottleneck for s

97 Crystal structure determination coupled with basic biochemical a

98 proteomics are far outpacing macromolecular structure determinations, creating information gaps betw

99 High-resolution structure determination crucially depends on the homogen

100 es significantly enhanced resolution in FRET structure determinations, demonstrated here in a study o

101 has found little use in atomic-level protein structure determination due to the uncertainty of residu

102 aspect of RiPP research is the difficulty of structure determination due to their architectural compl

103 dition to their key improvements for de novo structure determination, due to their smaller size these

104 zing novel families that emerge from protein structure determination efforts.

105 The resulting experimental inferential structure determination (EISD) method is size extensive

106 However, structure determination, even using de novo methods, rem

107 This is the first example of NMR crystal structure determination for a molecular compound of prev

108 Structure determination for amyloid fibrils presents man

109 d refinement has revolutionized the field of structure determination for large biomolecular systems.

110 tal structures that some of them have eluded structure determination for many years.

111 unanswered question due to the challenges of structure determination for membrane protein complexes.

112 e-residue couplings and demonstrate accurate structure determination for several proteins 6-41 kDa in

113 CA_C2195 was chosen for crystal structure determination for structure-based function ann

114 individual proteins are sufficient to drive structure determination for the whole complex.

115 The structure determination for this model system shows exce

116 al X-ray diffraction and represent the first structure determinations for compounds featuring B-C(6)C

117 This in turn allows structure determination from crystals much smaller and m

118 ive imaging with free-electron lasers allows structure determination from ensembles of weakly scatter

119 hod should enable rapid and reliable protein structure determination from near-atomic-resolution cryo

120 ed method that uses electron diffraction for structure determination from very small three-dimensiona

121 on sources it has become possible to perform structure determinations from the measurement of multipl

122 Crystal structure determination has long provided insight into s

123 Crystal structure determination has revolutionized modern scienc

124 The most informative SSCCs for structure determination, i.e., (3)J, were computed with

125 Most current approaches for structure determination improve cryo-ET resolution eithe

126 This enables high-resolution cryo-EM structure determination in a matter of days on a single

127 Structure determination in complex with hen egg white ly

128 a second refinement at 175 K and the crystal structure determination in the low temperature phase at

129 in the algorithms used for crystallographic structure determination in the Phenix and Crystallograph

130 d to virtually all areas of crystallographic structure determination including high-pressure studies,

131 Despite the advances in NMR, structure determination is often slow and constitutes a

132 Its crystallographic structure determination is the first for a nonheme iron

133 The golden age of ribosome structure determination is thus drawing to a close, and

134 range of specimens, single-particle cryo-EM structure determination is transforming structural biolo

135 The most powerful method for protein structure determination is X-ray crystallography which r

136 routinely employed at synchrotrons for novel structure determination, is now possible at X-ray FELs.

137 tional spectroscopy and single-crystal X-ray structure determination, making these compounds the firs

138 ture of the antibody-antigen complex, though structure determination may in many cases be impractical

139 Here, we describe a structure determination method that avoids the reconstru

140 Conventional three-dimensional (3D) structure determination methods require either multiple

141 Protein structure determination methods using magic-angle spinni

142 Two structure determination methods, based on the molecular

143 This represents a paradigm shift in structure determination methods, which we demonstrate wi

144 these superlattices pose a new challenge for structure determination methods.

145 makes them largely inaccessible to standard structure determination methods.

146 uration, and epitope mapping of 8H9 based on structure determination, modeling, and yeast display met

147 inst GT-3 NS3/4A protease is elucidated with structure determination, molecular dynamics simulations

148 pectroscopy in solution and by X-ray crystal-structure determination of (Bu4N)2P16 in the solid state

149 X-ray structure determination of (E)-1,2-bis{(E)-2-chloro-1-(c

150 A single-crystal X-ray structure determination of 6b-Ir shows a square-pyramida

151 We report the synthesis and total structure determination of a Au(24) nanocluster protecte

152 Here, we report the structure determination of a Au68NP at atomic resolution

153 Overall, the results represent the first structure determination of a bacterial diterpene cyclase

154 We report the synthesis and crystal structure determination of a gold nanocluster with 103 g

155 Here, we report the synthesis and total structure determination of a large box-shaped Ag67 nanoc

156 We report the structure determination of a large gold nanocluster form

157 Herein, we report the structure determination of a long unknown phase of glyci

158 We report the synthesis and structure determination of a new Au22 nanocluster coordi

159 We report the purification and structure determination of a quaternary translesion poly

160 ntial applications for the three-dimensional structure determination of a range of objects, ranging f

161 Structure determination of a resulting representative ph

162 ellet of V. harveyi led to the isolation and structure determination of a suite of eight amphi-entero

163 omolecular refinement approaches be used for structure determination of abiological molecular complex

164 This work has not only allowed the full structure determination of all of these natural products

165 Nuclear magnetic resonance (NMR) protein structure determination of an N-terminal 23-amino acid p

166 experimental demonstration of single-shot 3D structure determination of an object; in this case, indi

167 Structure determination of an unmutated ancestor antibod

168 By contrast, structure determination of asymmetric components remains

169 oelectron microscopy for the high-resolution structure determination of biomolecules in solution." Ac

170 Here we used (113)Cd NMR spectroscopy during structure determination of Bud31p, a 157-residue yeast p

171 drupolar nuclei and notably the atomic-scale structure determination of catalysis materials such as m

172 d with significant success to facilitate the structure determination of challenging macromolecules as

173 on has proven successful for high-resolution structure determination of challenging membrane proteins

174 Imminent structure determination of chemokine receptor CXCR4 moti

175 This discovery offers the prospect of facile structure determination of complex biological macromolec

176 community to guide and inform the process of structure determination of complex organic compounds, we

177 an attractive approach for three-dimensional structure determination of complex RNAs that are not ame

178 This work combines 3D atomic structure determination of crystal defects with DFT calc

179 Cryo-SAXS will thus enable structure determination of difficult-to-express proteins

180 We report the first experimental structure determination of dihydroxycarbene (HO-:C-OH),

181 n of these functionally essential states, 3D structure determination of excited states (ESs) of RNA r

182 ered a unique mechanism for this through the structure determination of four sequence-diverse M prote

183 Structure determination of free PLK4-PB3 and its STIL-CC

184 Recent successes in the structure determination of G-protein-coupled receptors (

185 Structure determination of gold nanoparticles (AuNPs) is

186 The traditional structure determination of gp41-M-MAT using the Xplor-NI

187 High-resolution X-ray structure determination of Haemophilus influenzae KDO8PP

188 Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spe

189 was recently used in our 2.1-A X-ray crystal structure determination of human RhCG.

190 zed HS-binding protein of interest, putative structure determination of isolated compounds by a hydro

191 We describe an approach to the structure determination of large proteins that relies on

192 Crystal structure determination of lithiated N-methylaniline wit

193 e data from three crystals and used them for structure determination of lysozyme by molecular replace

194 omogram averaging, and this technique allows structure determination of macromolecular complexes in s

195 ces in electron cryo-microscopy have enabled structure determination of macromolecules at near-atomic

196 Structure determination of mammalian integral membrane p

197 discs provide critical improvements for the structure determination of membrane proteins by NMR.

198 Recent advances in structure determination of membrane proteins have allowe

199 tion as weak-alignment media in solution NMR structure determination of membrane proteins in detergen

200 face, the method is generally applicable for structure determination of molecules with appropriate en

201 X-ray structure determination of monohydrazinium 3,5-bis(dinit

202 Here we have addressed this question by the structure determination of mouse TMEM16A by cryo-electro

203 ets to prevent flavivirus infection, and the structure determination of NS2B will help us to understa

204 rmostabilisation, could in theory facilitate structure determination of other membrane proteins by im

205 erformed a molecular dynamics simulation and structure determination of oxidized rubredoxin using the

206 Structure determination of PelB TPRs 8-11 enabled us to

207 Crystal structure determination of PenA and PenI reveals importa

208 e direction of biological cascades,8 and the structure determination of periodically positioned molec

209 The crystal structure determination of photosynthetic protein comple

210 ce assignment as well as the high-resolution structure determination of polytopic membrane proteins i

211 Structure determination of protein binding to noncrystal

212 that due to the difficulties in experimental structure determination of protein-protein complexes, th

213 perimental techniques for rapid and accurate structure determination of protein-protein complexes.

214 Structure determination of proteins and other macromolec

215 graphy will become an important tool for the structure determination of proteins that are difficult t

216 Structure determination of reactive species is a key ste

217 ormational flexibility currently hinders the structure determination of several classes of membrane r

218 icroscopy, biochemical analysis, and crystal structure determination of several RP subunits has yield

219 Our approach provides a paradigm for the structure determination of similarly complex macromolecu

220 strategy that uses macromolecule methods for structure determination of small molecules and their ass

221 adigm shift in the use of NMR active tags in structure determination of small molecules in complex mi

222 icroscopy and protein crystallography to the structure determination of subunits of multimeric comple

223 Structure determination of the Apo2L/TRAIL-DR5-AMG 655 t

224 Here we show, via the structure determination of the complex between Bax and a

225 Here we describe the structure determination of the Cry3A toxin found natural

226 our photomycolactones was elucidated via (1) structure determination of the four photoproducts in the

227 nd transcriptional analyses combined with PG structure determination of the guaA mutant enabled us to

228 Structure determination of the HIV-1 envelope glycoprote

229 capsid, consistent with a recent asymmetric structure determination of the MS2 virion.

230 Structure determination of the native trimeric AlnA to 2

231 ntal data have been used for the integrative structure determination of the p85alpha dimer.

232 ctively, in facilitating crystallization and structure determination of the Prdm14-Mtgr1 complex, or

233 The single-crystal X-ray structure determination of the presumably active catalyt

234 hemical synthesis and complete spectroscopic structure determination of the putative cross-link remna

235 The resulting structures enabled the structure determination of the two CO2-containing interm

236 Structure determination of these complexes in a purified

237 Despite recent progress in structure determination of these transporters, the confo

238 y, kinetic analysis was coupled with crystal structure determination of these WoA variants.

239 X-ray structure determination of three 11H-indolo[3,2-c]quinol

240 difficulties associated with high resolution structure determination of transmembrane proteins, addit

241 Here we report the structure determination of two different protein samples

242 Structure determination of unexpected products obtained

243 cocrystallizations are frequently applied to structure determinations of proteins, our study represen

244 The crystal structure determinations of the complexes of CPGRP-S wit

245 In this vein, we report the synthesis, structure determination, optical properties, and theoret

246 omics Center for Infectious Disease (SSGCID) structure determination pipeline.

247 addition to continuous rapid progress in RNA structure determination, probing, and biophysical studie

248 The method should streamline the cryo-EM structure determination process, providing accurate and

249 maps are the ultimate result of the cryo-EM structure determination process.

250 o large oligomeric systems than conventional structure-determination protocols, which often require a

251 The structure determinations provide valuable insight, at su

252 d with the same amino-acid sequence used for structure determination, providing a direct link between

253 NMR chemical shifts can be used for protein structure determination, rapid advances have recently be

254 This hybrid approach to structure determination requires that an atomic resoluti

255 Structure determinations reveal M-CO distances ranging f

256 The crystal structure determination revealed a zwitterionic diazoniu

257 X-ray structure determination reveals a remarkable "universal

258 A single-crystal X-ray structure determination reveals that O2 binds end-on to

259 ein lattices should enable new approaches to structure determination, sensing, and nanomaterial engin

260 The structure determinations showed that compounds GlcNAc an

261 X-ray crystal structure determination shows that the structural integr

262 om racemic mixtures also offer advantages in structure determination strategies.

263 Finally, NMR structure determinations suggested that MTP1 would be or

264 F), along with tools from the macromolecular structure determination suite PHENIX.

265 Through protein structure determination, targeted mutagenesis, high-thro

266 not readily accessible to any of the typical structure determination techniques such as smFRET, NMR,

267 chniques is much higher than that of protein structure determination techniques.

268 ray FEL data can be used for de novo protein structure determination, that is, without previous knowl

269 its position as the definitive method of MOF structure determination, these issues must be addressed

270 Due to difficulties in experimental structure determination, they are sparsely represented i

271 tion of the combination of three-dimensional structure determination through PCDI with a cryogenicall

272 mutant thioesterases were subjected to X-ray structure determination to confirm retention of the nati

273 t Center for Structural Genomics for crystal structure determination to explore novel protein sequenc

274 n of specimen preparation methods, assisting structure determination to high resolution with minimal

275 ues with applications ranging from molecular structure determination to human imaging.

276 from fast direct-electron detectors allowed structure determination to near-atomic resolution from 3

277 ion of crystals using the in meso method and structure determination to resolutions ranging from 2.4

278 does the focus in the field will shift from structure determination to understanding why the ribosom

279 escence imaging and higher-resolution atomic structure determination, to cover the full scale of cell

280 e maturation of cryo-EM as a high-resolution structure-determination tool, I believe there still are

281 ces in high throughput sequencing, chromatin structure determination, transcription factor and enhanc

282 Here, we realize routine structure determination using intrinsic anomalous scatte

283 ecent advances and future trends for protein structure determination using MAS SSNMR, as well as its

284 culties of growing high-quality crystals for structure determination using synchrotron radiation.

285 erential scanning calorimetry (DSC), crystal structure determination, UV-vis absorption, X-ray absorp

286 and MCD spectroscopies, single-crystal X-ray structure determination, vibrational spectroscopy, NMR a

287 The structure determination was aided by a novel approach to

288 Historically, room-temperature structure determination was succeeded by cryo-crystallog

289 ing its promise for experimental biomolecule structure determination where only semireliable data are

290 escribe a Bayesian interpretation of cryo-EM structure determination, where smoothness in the reconst

291 was rationalized through an X-ray cocrystal structure determination, which showed a flipped binding

292 was required to get spectra suitable for NMR structure determination, while the tag was required for

293 Overall, faster structure methods for structure determination will serve the natural products

294 regime, paving the way to nanometer-scale 3D structure determination with electrons.

295 ant reductive dehalogenase and, by combining structure determination with EPR (electron paramagnetic

296 the tenths of femtosecond time scale enables structure determination with no reduction or X-ray damag

297 to good quality crystals, suitable for X-ray structure determination with unprecedented probability l

298 outlining some key aspects in the process of structure determination, with the goal to encourage nonc

299 k also demonstrates the feasibility of local structure determination within large noncrystalline asse

300 mputationally intensive steps of its cryo-EM structure determination workflow.