ライフサイエンスコーパス: subcellular

1 The technology relies on controlling the subcellular activation of RhoA using the CRY2/CIBN light

2 with collagen-coupled agarose gels to detect subcellular activities of SFK and FAK in three-dimension

3 al and extracellular mechanical signals into subcellular activity patterns to control cellular contra

4 ls and fundamental mechanistic insights into subcellular adrenergic signalling in normal and patholog

5 odel, thus providing a means of bridging the subcellular and cellular phenomena in sickle cell diseas

6 ve platforms to precisely follow and measure subcellular and molecular events, are paving the way for

7 educe the time and human effort required for subcellular annotation and feature extraction.

8 cuticulosomes, as well as the molecular and subcellular architecture of cuticulosome positive hair c

9 ly establish the effects of the knockouts on subcellular architecture.

10 infections is their dependence on efficient subcellular assembly platforms serving replication, viri

11 lasma membrane efflux carriers that generate subcellular auxin gradients.

12 aining vacuole integrity required to balance subcellular BAK1 pools and BR receptor distribution.

13 How these subcellular/cellular events overcome source-sink factors

14 the same protein complex and display notable subcellular colocalization at presynaptic and postsynapt

15 Because each subcellular compartment in plants contains its own set o

16 heir intended substrates regardless of which subcellular compartment in the target cell they happen t

17 suggesting that sequestration of RNA to this subcellular compartment is both necessary and sufficient

18 A suite of targeted proteomics markers for subcellular compartment markers was developed, enabling

19 from the lysosomal to the zymogen-containing subcellular compartment of acinar cells and activation o

20 nerates EM contrast on a specific protein or subcellular compartment of interest.

21 In particular, it is unknown within what subcellular compartment pathogenic Htt acts and whether

22 nal delivery of intact drug into the desired subcellular compartment, however, it is critical that th

23 Subcellular compartmentalization of receptor signaling i

24 in recruitment and as diffusion barriers for subcellular compartmentalization.

25 g physiological stress, even without loss of subcellular compartmentalization.

26 xtracellular vesicles originate from diverse subcellular compartments and are released in the extrace

27 e signaling pathways, coordinated by several subcellular compartments and interactions between these

28 orchestrate signaling activity in different subcellular compartments at different timescales.

29 Some effectors have been found to enter subcellular compartments by mimicking host targeting seq

30 ng by a modified PageRank algorithm based on subcellular compartments information, with the ranking b

31 entify unrecognized constituents of distinct subcellular compartments refractory to biochemical isola

32 hat some GPCRs are also localized to various subcellular compartments such as the nucleus where they

33 h cytosolic targets and targets localized in subcellular compartments were investigated.

34 Hence, subcellular compartments with little but functionally re

35 APEX-RIP can isolate RNAs from a variety of subcellular compartments, including the mitochondrial ma

36 ovides a major advancement in fingerprinting subcellular compartments, with an increased potential to

37 ecific regulation of translation in specific subcellular compartments.

38 ut methods for mapping the transcriptomes of subcellular compartments.

39 cells, with the highly efficient labeling of subcellular components and the activation of prodrugs.

40 hnique for the precision removal of cells or subcellular components, as a tool to investigate mechani

41 a more stabilized structure is dependent on subcellular context, including local density and aging.

42 ables spontaneous, self-limiting patterns of subcellular contractility that can explore mechanical cu

43 lives, accumulation and gradual release from subcellular depots.

44 the importance of tRNA for translation, its subcellular distribution and diffusion properties in liv

45 teins with an Ig-like domain, share the same subcellular distribution and ectodomain shedding propert

46 Here we characterize the subcellular distribution and function of PS in the rod-s

47 e are signal transduction centers, yet their subcellular distribution and preservation in cardiac myo

48 Subcellular distribution and protein expression levels i

49 These data reveal a novel subcellular distribution for ZNF804A within somatodendri

50 ons, although GIRK2c achieved a more uniform subcellular distribution in pyramidal neurons and suppor

51 The subcellular distribution of (64)Cu was measured by cell

52 High expression or aberrant subcellular distribution of Ape1 has been detected in ma

53 Finally, an analysis of the subcellular distribution of C5aR and C5L2 indicates that

54 Recent insights into the subcellular distribution of cardiac and neuronal NaV iso

55 ration over the wounded area by altering the subcellular distribution of F-actin.

56 We also obtained the subcellular distribution of fast and slow diffusing tRNA

57 Although glucose did not affect the subcellular distribution of FRQ in the WT, highly elevat

58 We determined the subcellular distribution of individual machines, the sto

59 cular transport are critical for an adequate subcellular distribution of S-acylated Ras proteins.

60 tes provide singular information about H-Ras subcellular distribution that is required for GTPase sig

61 arious molecular weight forms of K15P, their subcellular distribution, and how these may differ in la

62 n membrane topology, protein processing, and subcellular distribution, and suggest that single- and d

63 the actin cytoskeleton, markedly alters Gag subcellular distribution, relocates sites of assembly, a

64 sponse to anaerobiosis by regulating RNase E subcellular distribution, RNase E enzymatic activity, an

65 mutants of H-Ras differed not only in their subcellular distribution, where both proteins localized

66 GABAAR complexes that explain GABAAR subunit subcellular distributions using mice and Xenopus laevis

67 mechanotransduction channels with different subcellular distributions.

68 However, little is known about their subcellular domain-dependent roles and responses to extr

69 Therefore, tango7 and dark define distinct subcellular domains of caspase activity.

70 ase adaptor proteins, and mutually exclusive subcellular domains of caspase activity.Caspases are kno

71 sphodiesterases (PDEs), localise to specific subcellular domains within which they control local cAMP

72 tually-exclusive and independently regulated subcellular domains.

73 ry of specific organelle cargoes to selected subcellular domains.

74 ficking events, cytoskeleton reorganization, subcellular dynamics and inter-organellar communication

75 rounding using a newly developed multi-scale subcellular element computational model that is calibrat

76 However, little is known how these subcellular events are coordinated.

77 The day we understand the time evolution of subcellular events at a level of detail comparable to ph

78 tablished ALS-related mutations, changed the subcellular expression and localization of RNAs within t

79 cterized the detailed regional, cellular and subcellular expression of GABAA (alpha1 , alpha2 , alpha

80 field fraction analyses were performed, and subcellular expression of GABAA receptor subunits was an

81 Subcellular fractionation and confocal microscopy demons

82 Using subcellular fractionation and immunofluorescence microsc

83 ion of the reporter gene CDKN1A, and in situ subcellular fractionation experiments demonstrated that

84 obtain quantitative data from microscopy and subcellular fractionation is experimentally difficult an

85 Fluorescence microscopy and subcellular fractionation methods are commonly employed

86 ents by confocal fluorescence microscopy and subcellular fractionation of endocytic vesicles on a Per

87 Subcellular fractionation of keratinocytes expressing E2

88 proteins identified by combining biochemical subcellular fractionation to the protein isolation metho

89 ctionation profiling, a method that combines subcellular fractionation with mass spectrometry, we ide

90 ntified and producer cells were subjected to subcellular fractionation.

91 ROC operates in various subcellular fractions including microsomes, mitochondria

92 as measured by incubating active human liver subcellular fractions with thyroid hormones (T4 and rT3

93 a levels, with reduced targeting to synaptic subcellular fractions.

94 A) in RNA transcripts by analyzing different subcellular fractions.

95 ings indicate that mitochondria can modulate subcellular functional specialization in photoreceptors.

96 romolecules with an "always on" reporter and subcellular imaging of endolysosomal escape by confocal

97 Interrogation of single SFs with subcellular laser ablation reveals that MLCK and ROCK qu

98 y combining single-cell micropatterning with subcellular laser ablation to probe the mechanics of sin

99 s these disparate conditions together at the subcellular level and can be exploited for broad curativ

100 tial distribution of the human proteome at a subcellular level can greatly increase our understanding

101 Substantial evidence at the subcellular level indicates that the spatial arrangement

102 At the subcellular level, these phenotypes were associated with

103 At the subcellular level, this model can simulate HbS polymeriz

104 g's modulus values for bulk rigidity, at the subcellular level, tissues are comprised of heterogeneou

105 tubules to be distinct and resolvable at the subcellular level.

106 nd miRNA expression in two ALS models at the subcellular level.

107 arise from interactions at the cellular and subcellular level.

108 Delivery of biomolecules to the correct subcellular locales is critical for proper physiological

109 proteins (gag-pol mRNAs) to distinct non-PM subcellular locales, such as cytoplasmic vesicles or the

110 Subcellular localization analysis showed that MYBS1 and

111 We found that O2 levels regulate the subcellular localization and channel activity of the pol

112 le, we demonstrate in human T cells that the subcellular localization and function of the protein tyr

113 ultiple stoichiometries exhibiting different subcellular localization and functional properties.

114 sms of AMPK regulation, including changes in subcellular localization and phosphorylation by non-cano

115 PH-domain mutants alter subcellular localization and result in decreased interac

116 Advanced technologies of lncRNA subcellular localization and silencing were used to iden

117 Because the subcellular localization and substrate specificity of th

118 and modifier Tet1 by analyzing their dynamic subcellular localization and the formation of the Tet ox

119 n channels depends critically on the precise subcellular localization and the number of channel prote

120 abietane olefin miltiradiene, but also their subcellular localization and, critically, genetic analys

121 ial of histone deacetylase 1 (HDAC1) and its subcellular localization are not fully understood.

122 that Ser-31 phosphorylation may regulate TH subcellular localization by enabling its transport along

123 tion into arrays modifies its own and ParC's subcellular localization dynamics, promoting their polar

124 provides a promising strategy for directing subcellular localization for therapeutic and fundamental

125 ough many web-servers for predicting protein subcellular localization have been developed, they often

126 euronal morphology and express proteins with subcellular localization indicative of mature neurons.

127 Protein subcellular localization is fundamental to the establish

128 ption, and suggests that regulation of CASZ1 subcellular localization may impact its function in norm

129 Direct perturbation of HIV-1 mRNA subcellular localization may represent a novel antiviral

130 We also putatively annotated and mapped the subcellular localization of 29 sulfoglycosphingolipids a

131 liferation and tumorigenesis by altering the subcellular localization of an essential YAP co-factor.

132 Subcellular localization of antigen, HLA molecules, and

133 eocytoplasmic partitioning, we monitored the subcellular localization of COP1 in a spa1234 quadruple

134 ides on the endosperm/scutellum boundary and subcellular localization of ferulate in the aleurone.

135 Examination of subcellular localization of FOXO protein via CRISPR-assi

136 When analyzing the subcellular localization of LmPRL-1 in promastigotes, am

137 Consistent with the subcellular localization of Mst, the intestinal defects

138 However, the mechanisms controlling the subcellular localization of PC1 and PC2 are poorly under

139 f phospholipids and the C-terminal domain in subcellular localization of ProP were explored.

140 ide fully recapitulated their effects on the subcellular localization of ProP.

141 methylation maintenance, likely by directing subcellular localization of proteins involved in this pr

142 Subcellular localization of PS-/SYS-GFP was observed usi

143 regulate both HCV genome replication and the subcellular localization of replication complexes.IMPORT

144 this study, we show that the expression and subcellular localization of Shh effectors and ciliary pr

145 tubule number and length correlated with the subcellular localization of SPBs rather than their age.

146 e NAc with associated changes in the nuclear subcellular localization of SRF and MAL.

147 lysis and confocal microscopy visualized the subcellular localization of T-bet and GATA-3.

148 gues and variants in vitro was compared with subcellular localization of the corresponding orthologue

149 An analysis of the subcellular localization of the DHARs in Arabidopsis lin

150 The subcellular localization of the fluorescently labeled me

151 can produce SPMs and assessed expression and subcellular localization of the key SPM biosynthetic enz

152 of cIAP1 and cIAP2 and observe differential subcellular localization of the two proteins in OSCC.

153 he Drosophila wing primordium typically show subcellular localization of the unconventional myosin Da

154 d by Nature Protocols in 2006) to enable the subcellular localization of thousands of proteins per ex

155 Remarkably, beta-catenin showed a reversed subcellular localization pattern: Although beta-catenin

156 of mRNA transcripts, protein expression, and subcellular localization revealed that KV1.5 is the majo

157 -dimensional protein structure and performed subcellular localization studies.

158 Subcellular localization study indicated that it is an i

159 ight-dependent protein isoforms with altered subcellular localization that help the plant respond met

160 eins and RNAs are tightly regulated in their subcellular localization to exert their local function.

161 Reticulon-1 subcellular localization to the periphery after exposure

162 These changes in CLU subcellular localization were also associated with Bax a

163 tion of the gene, its expression pattern and subcellular localization were characterized.

164 The amino acid residues modulating HDAC1 subcellular localization were identified by site-directe

165 ation in parasitic strategies (e.g. host and subcellular localization) or due to multiple transitions

166 re required for light-responsiveness of COP1 subcellular localization, and (2) they promote COP1 acti

167 lar cloning to examine how PTEN's stability, subcellular localization, and catalytic activity affect

168 twork-based analyses of domain associations, subcellular localization, and co-complex formation.

169 f HBx by HDM2 which regulates its stability, subcellular localization, and functions.

170 lar processes including signal transduction, subcellular localization, and regulation of enzymatic ac

171 ERalpha protein levels, subcellular localization, and transcriptional function w

172 ed information on tissue expression, protein subcellular localization, biological process, and molecu

173 that full-length BACE1, independently of its subcellular localization, exists as trimers in human cel

174 abidopsis using protein-protein interaction, subcellular localization, gene knockout, and bioinformat

175 the C-terminus of ATP8A2 in its expression, subcellular localization, interaction with its subunit C

176 eceptor-mediated endocytosis and appropriate subcellular localization, the identity of the cell surfa

177 ional methods exist to predict plant protein subcellular localization, they perform poorly for effect

178 To visualize mensacarcin's subcellular localization, we synthesized a fluorescent m

179 itored EV proteins, but they differ in their subcellular localization, with CD63 primarily in endosom

180 is oocytes, and their in planta cellular and subcellular localization.

181 ut ligand activation, effector coupling, and subcellular localization.

182 igase activity, substrate specification, and subcellular localization.

183 aying altered lipid phosphatase function and subcellular localization.

184 fast and effective method to annotate their subcellular localization.

185 sary for the light-controlled change in COP1 subcellular localization.

186 with unique target sequence specificity and subcellular localization.

187 respectively, enforced through differential subcellular localization.

188 d protein-membrane interactions, and protein subcellular localization.

189 conformational flexibility or changing their subcellular localization.

190 tability, catalytic activity, and/or altered subcellular localization.

191 affect receptor sensitivity without changing subcellular localization.

192 that FUEL-mLoc outperforms state-of-the-art subcellular-localization predictors.

193 In the study, we investigated subcellular localizations and assembly-promoting functio

194 d that the resulting isoforms have different subcellular localizations.

195 s and cellular processes, and showed diverse subcellular localizations.

196 ctive molecular hierarchy depending on their subcellular location and extracellular stimuli.

197 ce of mRNA translational repression and mRNA subcellular location for protein expression during B lym

198 However, the subcellular location for SOCE in muscle fibers has not b

199 r preferential NMDAR inhibition depending on subcellular location has not been investigated systemati

200 tilbenoid substrates, and we confirmed their subcellular location in the plastid by fluorescence micr

201 Subcellular location of NA-TLRs is a key determinant in

202 xpression of RD3 in different cell types and subcellular location of retina.

203 proteolytic activity, without affecting the subcellular location of the enzyme.

204 together with substantial differences in the subcellular location of this protein between the frog an

205 t at, or move between, two or more different subcellular location sites.

206 unique functions of Agap2 determined by its subcellular location.

207 ive abundance of these PTMs as a function of subcellular location.

208 ding proteins that it predicts share similar subcellular locations and residue charge profiles with t

209 oach to determine Cdc42 activity at specific subcellular locations and reveals new regulatory princip

210 roteins can be positioned rapidly at precise subcellular locations by local protein synthesis (LPS) t

211 es have implicated restricted and surprising subcellular locations in which miRNA biogenesis or activ

212 uggest that trafficking of LANA to different subcellular locations is a regulated phenomenon, which a

213 plasm and the nucleus, consistent with known subcellular locations of PIKKs.

214 Hh:GFP moved to basal subcellular locations prior to release from posterior co

215 independent of each other, to their separate subcellular locations.

216 This subcellular map can be used to refine existing protein-p

217 n patterns, expression-level divergence, and subcellular markers of functional diversification in the

218 The subcellular mechanisms by which cells sense ECM geometry

219 We examined the subcellular mechanisms involved in sarcoplasmic reticulu

220 To elucidate the subcellular mechanisms of contact guidance, we analyze q

221 Here we show that, besides these local subcellular mechanisms, the formation and maintenance of

222 itment of the viral RdRP and host factors to subcellular membrane microdomains enriched with specific

223 erging theme is the targeting of proteins to subcellular microdomains within bacterial cells, particu

224 In this study, we use intravital subcellular microscopy in live mice to study the role of

225 DNA representing SGPL1 mutations resulted in subcellular mislocalization of SGPL1.

226 Subcellular mislocalization of the microtubule-associate

227 ding to environmental cues, in part, through subcellular mRNA regulation.

228 Therefore, by combining the MS2 system and subcellular nanobody expression, we uncovered that maint

229 vestigate the spectral reflectance from this subcellular nanostructure and devise a new label-free te

230 and therapeutic products, while their use in subcellular organelle isolation remains underexploited.

231 e interaction between mitochondria and other subcellular organelles and for treatment of a variety of

232 ver, in plants, the existence of cell walls, subcellular organelles and the lack of stable cell lines

233 5P has been reported to localize to numerous subcellular organelles in heterologous expression studie

234 Enteric and other bacteria use subcellular organelles known as bacterial microcompartme

235 Viral components target subcellular organelles to access host machineries requir

236 entalized among different tissues, cells and subcellular organelles.

237 Emerging evidence suggests that the subcellular organization of contractile cytoskeletal net

238 elements as one of nature's most fascinating subcellular parasites.

239 -mediated endocytosis and contributes to the subcellular partitioning of charged polarity-regulating

240 ific localization patterns coincide with the subcellular patterns of IQD-dependent recruitment of CaM

241 To study subcellular PDGFR activity at membrane microdomains, thi

242 lus architecture remodeling), which measures subcellular peptidoglycan dynamics.

243 ession of zTrpa1b in sensory neurons allowed subcellular photo-activation, enabling light-dependent m

244 A similar alteration between the subcellular pools of the E2(WT) protein occurred upon Ch

245 vents can also contribute to localization of subcellular pools of this kinase.

246 as likely to occur as a result of a specific subcellular process and that it, therefore, should be po

247 sults indicate that the abnormalities in the subcellular processing of excess PMP22 elicit a detectab

248 e present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built

249 NPAS was combined with subcellular protein localization data, facilitating quan

250 used for protein labeling, visualization of subcellular protein localization, and detection of cell-

251 veals the joint dynamics of gene expression, subcellular protein localization, protein phosphorylatio

252 e image analysis of monolayer disruption and subcellular protein redistribution, we show that the mec

253 fundamentally altered our ability to resolve subcellular proteins, but improving on these techniques

254 tic acinar and inflammatory cells, undergoes subcellular redistribution and activation during experim

255 gulates the type I IFN pathway by inhibiting subcellular redistribution and effective signaling of ST

256 to the inner mitochondria membrane and that subcellular redistribution of ATP levels from the mitoch

257 Supramaximal caerulein stimulation induced subcellular redistribution of CTSD from the lysosomal to

258 For monitoring intra- and subcellular redox events, highly sensitive and specific

259 plasm and nucleus, localization to different subcellular regions can affect their therapeutic potency

260 opsin to activate the Gi pathway in defined subcellular regions of RAW cells, we show that in additi

261 Subcellular regulation of MT dynamics is spatially contr

262 epidermis is ideally suited for cellular and subcellular research, and their sensitivity to endogenou

263 havior, we monitored brainwide activity with subcellular resolution and identified a particularly act

264 target engagement and drug distribution with subcellular resolution in live cells and whole organisms

265 acile quantification of mRNA expression with subcellular resolution on a standard confocal microscope

266 m, we demonstrate local RI measurements with subcellular resolution on a standard TIRF microscope, wi

267 nformation of the cellular mRNA content with subcellular resolution within tissues.

268 r compartments, and to facilitate imaging at subcellular resolution without interference from the bio

269 rganoid function and behavior because of its subcellular resolution, penetration depth through the en

270 e mapping of the mouse brain at cellular and subcellular resolution.

271 mic metabolic changes in living animals with subcellular resolution.

272 orescent detection of nanoparticle fate with subcellular resolution.

273 thods to image large volumes of the brain at subcellular resolution.

274 nous metabolites to be visualized in 3D with subcellular resolution.

275 Subcellular RNA analysis in MeCP2-mutant neurons further

276 e evaluate quantitatively how factors at the subcellular scale (number of Ca wave initiation sites),

277 aused by differences in cellular plasticity, subcellular signaling pathways, and genetic expression.

278 owever, the primary pathogenic mechanism and subcellular site of action for mutant Htt are still uncl

279 ter Ca(2+)-CaM signaling modules to specific subcellular sites for precise regulation of Ca(2+)-depen

280 rs involved in these processes, but also the subcellular sites in which these processes are known or

281 is activated, as it accumulates in different subcellular sites with increasing cell density.

282 ve pathogenesis remains poorly understood, a subcellular spatial alteration in RNA metabolism is thou

283 detection of neural electrical activity with subcellular spatial resolution and millisecond-timescale

284 sualizing SNARE complexes in live cells with subcellular spatial resolution.

285 The basis for maintaining distinct subcellular sphingolipid levels in the presence of membr

286 on of the cytoskeleton, suggesting that this subcellular structure could be targeted for therapeutic

287 ochemical diversity and alter the associated subcellular structure required for functionality of the

288 ion of the tagged protein to the appropriate subcellular structure, pluripotency-marker expression, a

289 Dendritic filopodia are actin-filled dynamic subcellular structures that sprout on neuronal dendrites

290 Labeling and visualizing cells and subcellular structures within thick tissues, whole organ

291 align and correctly position a wide range of subcellular structures, including actin-based dynamic pr

292 st, making it difficult to examine cells and subcellular structures.

293 inguish between these mechanisms by applying subcellular targeting using a soft X-ray microbeam in co

294 d in more precise reconstructions of complex subcellular targets.

295 se system can mobilize and coalesce multiple subcellular trafficking circuitries to combat infections

296 Here we provide direct evidence that mRNA subcellular trafficking plays an important role in regul

297 Subcellular trafficking typically involves multiple Rabs

298 entity, number, position, or strength on Rev subcellular trafficking, viral RNA nuclear export, and i

299 In hASMCs, acute treatment with BK triggered subcellular translocation of ARHGEF1 and RhoA and enhanc

300 se shape has been proposed to be a result of subcellular variations in expansion rate that induce loc