ライフサイエンスコーパス: subcloning

1 ene to be expressed without any intermediate subcloning.

2 (AC hydrolase), was highly overexpressed by subcloning.

3 s identified by whole plasmid sequencing and subcloning.

4 products in a single sequencing run without subcloning.

5 through automation followed by single-colony subcloning.

6 APEx strategies without the need for further subcloning.

7 xperiments to be carried out without further subcloning.

8 efficient recombination linkers for fragment subcloning.

9 requires in vitro mutagenesis together with subcloning.

10 can be screened by PECS without the need for subcloning.

11 enerate prion-infected lines without further subcloning.

12 al helper virus in transfected cells without subcloning.

13 re determined after amplification by PCR and subcloning.

14 of DNA fragments is a standard procedure for subcloning analysis and for generating restriction maps.

15 Subcloning analysis showed that after serial passaging,

16 sduction conditions, followed by single-cell subcloning and a round of antibiotic selection, we find

17 Subcloning and activity analysis localized the genes for

18 and all in vitro manipulations required for subcloning and allows the rapid construction of multiple

19 Subcloning and complementation analysis localized the co

20 n the interval were then examined further by subcloning and complementation analysis.

21 Subcloning and complementation experiments localized the

22 Using subcloning and complementation experiments, ptxR was loc

23 The promoter was localized by subcloning and demonstrated to be strongly autorepressed

24 ing polymerase chain reaction, combined with subcloning and DNA sequencing techniques, the cDNAs that

25 The vector was tested by subcloning and expressing genomic and cDNA clones.

26 Subcloning and mutagenesis defined a genetic locus, desi

27 These genes were localized by subcloning and mutagenesis on a previously isolated cosm

28 Subcloning and mutagenesis studies localized the DNA con

29 Subcloning and nucleotide sequence analysis determined t

30 Subcloning and physical mapping localized the new gene (

31 ary sorting strategy that, in the absence of subcloning and protein purification, can distinguish dif

32 Subsequent subcloning and sequencing of a 2.3 kb BamHI BglII fragme

33 lts of quasispecies complexity determined by subcloning and sequencing of amplicon products from the

34 Through repeated cycles of amplification, subcloning and sequencing of multiple clones per amplico

35 Subcloning and sequencing of ORF15 of RPGR in female sub

36 gous patient (8 kb) by long PCR, followed by subcloning and sequencing of regions flanking the deleti

37 Subcloning and sequencing of the COX17 gene revealed tha

38 ubcloned in pBluescript and used for further subcloning and sequencing of the genes.

39 Subcloning and sequencing of the oxidase gene on a 6.5-k

40 Subcloning and sequencing of these PCR products indicate

41 Subcloning and sequencing showed that whiD encodes a hom

42 Subcloning and sequencing showed that whiK encodes a mem

43 Subcloning and sequencing showed that whiN encodes a mem

44 Sanger sequencing but were validated through subcloning and subsequent sequencing of the subcloned DN

45 ithin 'finished' genomes are recalcitrant to subcloning and suggests that the paired-end-sequenced fo

46 Results from subcloning and transposon mutagenesis indicated that the

47 Subcloning and transposon mutagenesis revealed a 5.6-kb

48 n human colon organoids, followed by delayed subcloning and whole-genome sequencing.

49 The results of subclonings and sequence analyses identified two genes,

50 and many in vitro manipulations required for subcloning, and allows for the rapid construction of mul

51 Additional library screening, subcloning, and complementation experiments showed that

52 Restriction enzyme mapping, subcloning, and DNA sequencing analysis of recombinant p

53 ction fragment length polymorphism analysis, subcloning, and DNA sequencing.

54 isolated and subjected to end point RT-PCR, subcloning, and sequencing.

55 DNA sequencing, subcloning, and serum adsorption experiments identified

56 Using a recently developed subcloning approach to detect stable new integrations of

57 n frequency in LMH 66-1 DSL cells by using a subcloning approach.

58 sting that many of these changes were PCR or subcloning artifacts rather than mutations in the tumor

59 Subcloning, complementation of trp strains, nucleotide s

60 oncogenic transformation, exhibited enhanced subcloning efficiency at low density, and resisted both

61 ue, that can be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authe

62 Subcloning experiments demonstrated that alrA was necess

63 Hybridization analysis and subcloning experiments showed that the P97 adhesin-encod

64 ty reagents and bypassing the time-consuming subcloning experiments.

65 eling and flow cytometry, obviating separate subcloning, expression, and purification steps and there

66 for variants present at unequal read depths, subcloning followed by colony sequencing.

67 After several round of subcloning followed by transfection, oriP was mapped to

68 t eliminates the need for post-amplification subcloning following each cycle of mutagenesis.

69 By selectively subcloning genes found in this insert into an Escherichi

70 Two expression constructs were prepared by subcloning genomic DNA fragments (one from the control s

71 Transposon insertion mutagenesis and subcloning identified a 5.7-kb region of BamHI fragment

72 Insertional mutagenesis and subcloning identified a single gene responsible for the

73 riction patterns and were chosen for further subcloning into a pBluescript vector.

74 enced, and genetically engineered by PCR for subcloning into expression vectors that contain human co

75 containing appropriate restriction sites for subcloning into pTETnirB, which contains the nirB promot

76 or agglutinin-like adhesin) was localized by subcloning it into a 5-kb XbaI fragment which retained t

77 The function of PKCdeltaVIII was examined by subcloning it into an expression vector and raising an a

78 Subcloning normal and mutant dgk yielded homodimeric dGK

79 Further subcloning of a 148 bp BamHI/EcoRI fragment resulted in

80 Subcloning of a 4.0-kb BclI fragment revealed the presen

81 Subcloning of an activation region (-4876 to -3453 nucle

82 ovides an effective strategy for directional subcloning of DNA fragments from Bacteria Artificial Chr

83 UCS75, has been constructed to permit facile subcloning of DNA from the multiple cloning sites of the

84 Subcloning of DNA from the valanimycin-producing cosmid

85 ned Escherichia coli plasmids for the facile subcloning of fusions to the green fluorescent protein f

86 Further subcloning of IC3.0 demonstrated that a smaller fragment

87 Subcloning of kappa B cis-acting elements of VCAM-1, E-s

88 Subcloning of parental PAX3-FKHR/MYCN-transduced myoblas

89 After subcloning of smaller portions of the AB capsid coding s

90 Subcloning of the DcR2 p53BS into a luciferase reporter

91 of PCR products and the subsequent automatic subcloning of the DNA segment into new vector backbones

92 This article reports subcloning of the drrA and drrB genes into Escherichia c

93 Subcloning of the drrAB genes in Escherichia coli has pr

94 PCR mapping and partial PCR mediated subcloning of the ER cDNA were used to demonstrate that

95 Further, to facilitate the subcloning of the fragment cloned into other targeting o

96 Subcloning of the individual ORFs demonstrated that ORF3

97 y (R24) and rabbit complement and subsequent subcloning of the surviving cells, resulting in the deri

98 Subcloning of the Ti plasmid indicated that the virA loc

99 Upon subcloning of the YAC contig into P1s and BACs, we have

100 Subcloning of these sequences into the 5' UTR of a const

101 phenotype of the transferase mutant; further subcloning of this fragment yielded a smaller one contai

102 Subcloning of this gene set in mammalian expression vect

103 Subcloning of this ORF demonstrated that it was sufficie

104 Subcloning of Thy-1(+) cells from OC-activated livers yi

105 ntation and spacing can be directly used for subcloning or other applications without further treatme

106 al candidate PEC cell lines were obtained by subcloning outgrowths of cells from capsulated glomeruli

107 We report here the subcloning, overexpression, and a robust but novel metho

108 By subcloning portions of the eibA and eibF genes, we have

109 The conventional hybridoma screening and subcloning process is generally considered to be one of

110 from mixtures and directly sequenced without subcloning, purification or denaturation.

111 phosphite oxidation deleted (based upon the subcloning results) lost the ability to oxidize either p

112 M. smegmatis genomic library and subsequent subcloning reveal that the defect lies within the M. sme

113 nner, and furthermore, ChIP-on-chip and ChIP subcloning reveal that wild-type and mutant Htt exhibit

114 Subcloning revealed that YhiX, a putative AraC/XylR fami

115 ces were determined by direct sequencing and subcloning sequencing.

116 Subcloning, sequencing, and protein sequence determinati

117 unctionally evaluated relative to MDR1 after subcloning SPGP cDNA into a retroviral bicistronic vecto

118 -50bp) between donor and target DNA, and one subcloning step that we can efficiently manipulate BACs

119 rounds of mutagenesis and screening and one subcloning step yielded the P450 2B1 quadruple mutant V1

120 -targeted regions, thereby bypassing tedious subcloning steps.

121 We now demonstrate by subcloning the 3' untranslated regions into wild-type TY

122 ion of high-titer virus without the need for subcloning the producer line.

123 These obstacles were overcome by directly subcloning the target region after release by Achilles'

124 Subcloning, transposon mutagenesis, and DNA sequence ana

125 body variable region fragments together in a subcloning vector.

126 the culture medium in a subsequent cycle of subcloning was accompanied by a reversion back towards t

127 By expressing the products of PCR subcloning, we identified a 146-amino acid region of the

128 ing, transcriptional fusion mutagenesis, and subcloning were used to identify tfuA, a gene involved i

129 d stably once every three generations during subcloning whereas wild-type DHBV integrated only once e

130 of nearly 500 kb spanning the MYCN locus by subcloning yeast artificial chromosomes into cosmids.