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1 ene to be expressed without any intermediate subcloning.
2  (AC hydrolase), was highly overexpressed by subcloning.
3  products in a single sequencing run without subcloning.
4 APEx strategies without the need for further subcloning.
5 xperiments to be carried out without further subcloning.
6 s identified by whole plasmid sequencing and subcloning.
7 efficient recombination linkers for fragment subcloning.
8  requires in vitro mutagenesis together with subcloning.
9 can be screened by PECS without the need for subcloning.
10 through automation followed by single-colony subcloning.
11 enerate prion-infected lines without further subcloning.
12 al helper virus in transfected cells without subcloning.
13 re determined after amplification by PCR and subcloning.
14 y was to search for the elusive haplotype by subcloning a DNA fragment of 177 bp from 355 subjects wi
15 of DNA fragments is a standard procedure for subcloning analysis and for generating restriction maps.
16                                              Subcloning analysis showed that after serial passaging,
17 sduction conditions, followed by single-cell subcloning and a round of antibiotic selection, we find
18                                              Subcloning and activity analysis localized the genes for
19  and all in vitro manipulations required for subcloning and allows the rapid construction of multiple
20                                              Subcloning and complementation analysis localized the co
21 n the interval were then examined further by subcloning and complementation analysis.
22                                              Subcloning and complementation experiments localized the
23                                        Using subcloning and complementation experiments, ptxR was loc
24                The promoter was localized by subcloning and demonstrated to be strongly autorepressed
25 ing polymerase chain reaction, combined with subcloning and DNA sequencing techniques, the cDNAs that
26                     The vector was tested by subcloning and expressing genomic and cDNA clones.
27                                              Subcloning and mutagenesis defined a genetic locus, desi
28                These genes were localized by subcloning and mutagenesis on a previously isolated cosm
29                                              Subcloning and mutagenesis studies localized the DNA con
30                                              Subcloning and nucleotide sequence analysis determined t
31                                              Subcloning and physical mapping localized the new gene (
32 ary sorting strategy that, in the absence of subcloning and protein purification, can distinguish dif
33                                   Subsequent subcloning and sequencing of a 2.3 kb BamHI BglII fragme
34 lts of quasispecies complexity determined by subcloning and sequencing of amplicon products from the
35    Through repeated cycles of amplification, subcloning and sequencing of multiple clones per amplico
36                                              Subcloning and sequencing of ORF15 of RPGR in female sub
37 gous patient (8 kb) by long PCR, followed by subcloning and sequencing of regions flanking the deleti
38                                              Subcloning and sequencing of the COX17 gene revealed tha
39 ubcloned in pBluescript and used for further subcloning and sequencing of the genes.
40                                              Subcloning and sequencing of the oxidase gene on a 6.5-k
41                                              Subcloning and sequencing of these PCR products indicate
42                                              Subcloning and sequencing showed that whiD encodes a hom
43                                              Subcloning and sequencing showed that whiK encodes a mem
44                                              Subcloning and sequencing showed that whiN encodes a mem
45 ommonly used genotyping methods that rely on subcloning and short-read or Sanger sequencing.
46 Sanger sequencing but were validated through subcloning and subsequent sequencing of the subcloned DN
47 ithin 'finished' genomes are recalcitrant to subcloning and suggests that the paired-end-sequenced fo
48                                 Results from subcloning and transposon mutagenesis indicated that the
49                                              Subcloning and transposon mutagenesis revealed a 5.6-kb
50 n human colon organoids, followed by delayed subcloning and whole-genome sequencing.
51                               The results of subclonings and sequence analyses identified two genes,
52 and many in vitro manipulations required for subcloning, and allows for the rapid construction of mul
53                Additional library screening, subcloning, and complementation experiments showed that
54                  Restriction enzyme mapping, subcloning, and DNA sequencing analysis of recombinant p
55 ction fragment length polymorphism analysis, subcloning, and DNA sequencing.
56  isolated and subjected to end point RT-PCR, subcloning, and sequencing.
57                              DNA sequencing, subcloning, and serum adsorption experiments identified
58                   Using a recently developed subcloning approach to detect stable new integrations of
59 n frequency in LMH 66-1 DSL cells by using a subcloning approach.
60 sting that many of these changes were PCR or subcloning artifacts rather than mutations in the tumor
61                                              Subcloning, complementation of trp strains, nucleotide s
62 oncogenic transformation, exhibited enhanced subcloning efficiency at low density, and resisted both
63 ue, that can be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authe
64                                              Subcloning experiments demonstrated that alrA was necess
65                   Hybridization analysis and subcloning experiments showed that the P97 adhesin-encod
66 ty reagents and bypassing the time-consuming subcloning experiments.
67 eling and flow cytometry, obviating separate subcloning, expression, and purification steps and there
68 for variants present at unequal read depths, subcloning followed by colony sequencing.
69                       After several round of subcloning followed by transfection, oriP was mapped to
70 t eliminates the need for post-amplification subcloning following each cycle of mutagenesis.
71                               By selectively subcloning genes found in this insert into an Escherichi
72   Two expression constructs were prepared by subcloning genomic DNA fragments (one from the control s
73         Transposon insertion mutagenesis and subcloning identified a 5.7-kb region of BamHI fragment
74                  Insertional mutagenesis and subcloning identified a single gene responsible for the
75 riction patterns and were chosen for further subcloning into a pBluescript vector.
76 irectly synthesized as DNA that is ready for subcloning into appropriate delivery vectors, for both i
77 enced, and genetically engineered by PCR for subcloning into expression vectors that contain human co
78 containing appropriate restriction sites for subcloning into pTETnirB, which contains the nirB promot
79 or agglutinin-like adhesin) was localized by subcloning it into a 5-kb XbaI fragment which retained t
80 The function of PKCdeltaVIII was examined by subcloning it into an expression vector and raising an a
81                                              Subcloning normal and mutant dgk yielded homodimeric dGK
82                                      Further subcloning of a 148 bp BamHI/EcoRI fragment resulted in
83                                              Subcloning of a 4.0-kb BclI fragment revealed the presen
84                                              Subcloning of an activation region (-4876 to -3453 nucle
85 ovides an effective strategy for directional subcloning of DNA fragments from Bacteria Artificial Chr
86 UCS75, has been constructed to permit facile subcloning of DNA from the multiple cloning sites of the
87                                              Subcloning of DNA from the valanimycin-producing cosmid
88 ned Escherichia coli plasmids for the facile subcloning of fusions to the green fluorescent protein f
89                                      Further subcloning of IC3.0 demonstrated that a smaller fragment
90                                              Subcloning of kappa B cis-acting elements of VCAM-1, E-s
91                                              Subcloning of parental PAX3-FKHR/MYCN-transduced myoblas
92                                        After subcloning of smaller portions of the AB capsid coding s
93                                              Subcloning of TCR-alpha/beta cDNAs from CD8EXP into Jurk
94                                              Subcloning of the DcR2 p53BS into a luciferase reporter
95 of PCR products and the subsequent automatic subcloning of the DNA segment into new vector backbones
96                         This article reports subcloning of the drrA and drrB genes into Escherichia c
97                                              Subcloning of the drrAB genes in Escherichia coli has pr
98         PCR mapping and partial PCR mediated subcloning of the ER cDNA were used to demonstrate that
99                   Further, to facilitate the subcloning of the fragment cloned into other targeting o
100                                              Subcloning of the individual ORFs demonstrated that ORF3
101 y (R24) and rabbit complement and subsequent subcloning of the surviving cells, resulting in the deri
102                                              Subcloning of the Ti plasmid indicated that the virA loc
103                                         Upon subcloning of the YAC contig into P1s and BACs, we have
104                                              Subcloning of these sequences into the 5' UTR of a const
105 phenotype of the transferase mutant; further subcloning of this fragment yielded a smaller one contai
106                                              Subcloning of this gene set in mammalian expression vect
107                                              Subcloning of this ORF demonstrated that it was sufficie
108                                              Subcloning of Thy-1(+) cells from OC-activated livers yi
109 ntation and spacing can be directly used for subcloning or other applications without further treatme
110 al candidate PEC cell lines were obtained by subcloning outgrowths of cells from capsulated glomeruli
111                           We report here the subcloning, overexpression, and a robust but novel metho
112                                           By subcloning portions of the eibA and eibF genes, we have
113     The conventional hybridoma screening and subcloning process is generally considered to be one of
114 from mixtures and directly sequenced without subcloning, purification or denaturation.
115  phosphite oxidation deleted (based upon the subcloning results) lost the ability to oxidize either p
116  M. smegmatis genomic library and subsequent subcloning reveal that the defect lies within the M. sme
117 nner, and furthermore, ChIP-on-chip and ChIP subcloning reveal that wild-type and mutant Htt exhibit
118                                              Subcloning revealed that YhiX, a putative AraC/XylR fami
119 ces were determined by direct sequencing and subcloning sequencing.
120                                              Subcloning, sequencing, and protein sequence determinati
121                                           By subcloning single daughter cells in culture, we show tha
122 unctionally evaluated relative to MDR1 after subcloning SPGP cDNA into a retroviral bicistronic vecto
123 -50bp) between donor and target DNA, and one subcloning step that we can efficiently manipulate BACs
124  rounds of mutagenesis and screening and one subcloning step yielded the P450 2B1 quadruple mutant V1
125 -targeted regions, thereby bypassing tedious subcloning steps.
126                        We now demonstrate by subcloning the 3' untranslated regions into wild-type TY
127 ion of high-titer virus without the need for subcloning the producer line.
128    These obstacles were overcome by directly subcloning the target region after release by Achilles'
129                                              Subcloning these late passage cells to clonal density, t
130                                              Subcloning, transposon mutagenesis, and DNA sequence ana
131 body variable region fragments together in a subcloning vector.
132  the culture medium in a subsequent cycle of subcloning was accompanied by a reversion back towards t
133            By expressing the products of PCR subcloning, we identified a 146-amino acid region of the
134 ing, transcriptional fusion mutagenesis, and subcloning were used to identify tfuA, a gene involved i
135 d stably once every three generations during subcloning whereas wild-type DHBV integrated only once e
136  of nearly 500 kb spanning the MYCN locus by subcloning yeast artificial chromosomes into cosmids.

 
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