ライフサイエンスコーパス: subconductance

1 n probability severalfold without inducing a subconductance.

2 potential has little effect on gating of the subconductance.

3 annel opening to the full-size versus a 3-pS subconductance.

4 states referred to as the full conductance, subconductance 1, and subconductance 2 states.

5 the full conductance, subconductance 1, and subconductance 2 states.

6 , p(681-690), and p(681-685) induced similar subconductances and long-lasting channel closings in ske

7 +)) RyRs, cytosolic RR induced a predominant subconductance at a positive but not negative holding po

8 However, analysis of K channel subconductance behavior has suggested a strict coupling

9 Fast closings and overt subconductance behavior of the wild-type I(Ks) channel r

10 ced the RyR1 channel P(o) but did not rescue subconductance behavior.

11 74 of 88 RyR3 channels exhibited pronounced subconductance behavior.

12 ts suggest that RR inhibits RyRs and induces subconductances by binding to cytosolic and lumenal site

13 fted to pH approximately 7.8), such that the subconductance channels are relatively quiescent at phys

14 This increase in P(o) for the subconductance channels by alkalinization is not associa

15 Open probability for the subconductance channels can then be increased by intrace

16 the frequency and decrease the open time of subconductance channels, while oxidizing conditions decr

17 o an increase in the occurrence of smaller, 'subconductance' currents with the higher concentrations

18 ite their greater frequency, the duration of subconductance events is so short relative to the main s

19 neither reduced P(o) nor recovered multiple subconductance gating.

20 The major subconductance had a current amplitude of 52% of fully o

21 t, 10 nm to 1 microm sMCa induced long lived subconductances having 48% of the characteristic full op

22 onductance states proportional to pA-induced subconductances (i.e. 43% of pA-modified s1 and s2 subst

23 Lumenal RR induced a major subconductance in cardiac RyR at negative but not positi

24 Cytosolic and lumenal RR also induced subconductances in Ca(2+)-conducting skeletal and cardia

25 not positive holding potentials and several subconductances in skeletal RyR.

26 nce of glow amplitudes parallels that of the subconductances induced by these ryanoids in single-chan

27 (Q10=2.4) drives AMPA channels with multiple subconductances into the higher-conducting states at hig

28 ease in the percent of channel openings to a subconductance level approximately 60% of the maximal si

29 ocus in the pore when the channel opens to a subconductance level as compared to the fully open level

30 o a 23 pS main conductance level and a 19 pS subconductance level, with infrequent openings to a 27 p

31 availability and channel openings to various subconductance levels (sublevels) as well as the fully o

32 ild-type human potassium channel, long-lived subconductance levels coupled to activation are a key fe

33 ong multiple amplitude levels reminiscent of subconductance levels of ion channels.

34 he approach is applicable to data containing subconductance levels or multiple channels and permits s

35 uency in mdx muscle and reflect occupancy of subconductance levels seen during complete activations.

36 ut mice, MS channels also spend more time at subconductance levels than the fully open state.

37 he wild-type (WT) channel rarely visited the subconductance levels under control conditions.

38 end approximately 60% of the time at smaller subconductance levels, often failing to reach the fully

39 urin altered the amplitude of single-channel subconductance levels, weighted mean unitary current, me

40 mide analogues with no obvious occurrence of subconductance levels.

41 state and rapid transitions between multiple subconductance levels.

42 lved to channel currents with at least three subconductance levels.

43 ully open state, while reducing occupancy of subconductance levels.

44 channel showed MS channels fluctuate between subconductance levels.

45 f single-channel gating and produce distinct subconductance levels.

46 partially modified channels exhibit distinct subconductance levels.

47 arations indicated that WT ML1 is a multiple subconductance non-selective cation channel whose functi

48 a main conductance of 37.9 +/- 1.1 pS and a subconductance of 17.8 +/- 0.7 pS, with frequent transit

49 The RR-related subconductances of cardiac RyR showed a nonlinear voltag

50 penings but not for the increase in receptor subconductance opening, thereby supporting the two-bindi

51 Full-size and subconductance openings of both mutant and wild-type cha

52 esence of a nonhydrolyzable ATP analog, with subconductance openings significantly shortened by ATPga

53 cysteines in NBD2 decreased the frequency of subconductance openings.

54 idizing conditions decrease the frequency of subconductance openings.

55 ed gates because of the channels dwelling in subconductance rather than closed states.

56 annel, with low concentrations stabilizing a subconductance state and high concentrations abolishing

57 In addition, a subconductance state at 124 +/- 5 pS was identified.

58 A prominent residual or subconductance state corresponding to 43 +/- 4.2 pS (10

59 The s1 subconductance state does not require either salt bridge

60 The occupancy of this subconductance state may explain the differences in chan

61 hanges in PSAC gating with the addition of a subconductance state not present in wild-type channels.

62 attributed to the formation of a long-lived subconductance state of MscL following channel opening.

63 records were filtered at 50 Hz, a very small subconductance state of murine CFTR was observed that ha

64 has a conductance of 4-7 pS, similar to the subconductance state of the second channel.

65 ar to those of the main open state and lower subconductance state of WT Kir2.1; however, the frequenc

66 +, and driven by ryanodine into a long-lived subconductance state that represented approximately 40 %

67 Kir2.1, but the total duration of the lower subconductance state was 3-5 times longer.

68 The occurrence of the lower subconductance state was reduced in the absence of extra

69 A subconductance state with an amplitude 70% that of the m

70 Average Po for the 2-3-pS subconductance state, estimated from the area under the

71 so by locking channels into the prominent s2 subconductance state, suggesting that the most stable co

72 vestigated whether murine CFTR may open to a subconductance state.

73 It has a high open probability and a subconductance state.

74 en this open state and a approximately 65-pS subconductance state.

75 transition from the fully open state to the subconductance state.

76 contributes to maintaining a stable s2 open subconductance state.

77 12)-diketopyridylryanodine readily induced a subconductance state.

78 probability (P(o)) but gives rise to a ROMK1 subconductance state.

79 tivity for the channels via emergence of the subconductance state.

80 ry rare 13 pS step transitions to one of two subconductance states (26 and 13 pS).

81 nnel fluctuates between two clearly resolved subconductance states (alpha and beta).

82 ymmetry of transitions between the main- and subconductance states - a characteristic of NR1/NR2D-con

83 t observation of blocking events as distinct subconductance states and for the first time demonstrate

84 characteristic pattern of transition between subconductance states and reduced sensitivity to Mg2+ bl

85 cation-selective channel exhibited multiple subconductance states and was blocked by high concentrat

86 Subconductance states are a commonly observed feature of

87 ation and probability of occurrence of these subconductance states but did not greatly alter their re

88 ding of the structural basis for ion channel subconductance states further highlight challenges that

89 sites is not required for ryanodine-induced subconductance states in RyR1.

90 ner and induced the appearance of long lived subconductance states in skeletal RyRs reconstituted int

91 -) VSMCs, CPA-induced channel currents had 3 subconductance states of 14, 32, and 53 pS.

92 evoked cation channel currents (I(cat)) with subconductance states of about 18, 34 and 51 and 68 pS,

93 These slow conversions among subconductance states of the CFTR channel were affected

94 Subconductance states of the channel may result from alt

95 urn control the open probability of discrete subconductance states of the intact ion channel.

96 cause the channel to persist in long-lived, subconductance states or, at high ligand concentrations,

97 sMCa stabilized additional subconductance states proportional to pA-induced subcond

98 ate conductance of 140 +/- 8 pS and multiple subconductance states ranging from < or =10 pS to 60 pS.

99 ound to exhibit more frequent transitions to subconductance states than the native RyR2 channels and

100 and the absence of either voltage gating or subconductance states upon D-alanine substitution suppor

101 These subconductance states were also observed with wild type

102 t, at negative potentials, brief sojourns to subconductance states were apparent.

103 In ten of thirty granule cells, clear subconductance states were observed with a mean conducta

104 The magnitudes and distributions of subconductance states were studied in chloride channels

105 The channel has six subconductance states with a maximum conductance of 130

106 nopus oocytes, are characterized by multiple subconductance states with only brief transient openings

107 RyRs, induced the appearance of long-lasting subconductance states, and markedly slowed the spontaneo

108 corresponding to the main open state and two subconductance states, were identified in WT Kir2.1 chan

109 2 showed that four intrinsic, non-stochastic subconductance states, which followed a staircase behavi

110 Subconductance states, which predominate in FKBP12.6-str

111 eptor-channel complex, and showed long-lived subconductance states.

112 or (CFTR) chloride channel exhibits multiple subconductance states.

113 s weakly cation-selective and showed several subconductance states.

114 cal gating behavior with multiple and stable subconductance states.

115 non-selective cation channel, with multiple subconductance states.

116 ing in leaky, unregulated channels gating in subconductance states.

117 ng between a nonconducting state and various subconductance states.

118 large increases in P(o), and no evidence of subconductance states.

119 lular Na(+), high conductance, and prominent subconductance states.

120 states may account for the observed multiple subconductance states.

121 , mecamylamine and tetracaine induced unique subconductance states.

122 single Ca(2+) release channels to prolonged subconductance states.

123 Assuming the ratio of full conductance to subconductance to be the same in the fibers as in bilaye

124 els that open almost exclusively to the 3-pS subconductance, while mutations of cysteines in NBD2 dec