ライフサイエンスコーパス: subculture

1 smooth muscle cells in vivo and in vitro (in subculture).

2 Many of the differences persist through subculture.

3 cells placed in culture, and did not require subculture.

4 nonadherent, smooth phenotype upon in vitro subculture.

5 se obtained from panels inoculated following subculture.

6 ower, were transformed, and none reverted on subculture.

7 ansformed cells and a reduced growth rate on subculture.

8 edium, growth temperature, and use of serial subculture.

9 m selective media and subsequently from pure subculture.

10 tly, typically at approximately 9 days after subculture.

11 (12.8%) were positive for S. agalactiae upon subculture.

12 ation assays were performed initially and at subculture.

13 The remaining stroma was digested and subcultured.

14 in G1 phase unless the irradiated cells are subcultured.

15 riod, and the resulting clones were serially subcultured.

16 resistant cells were selected, expanded, and subcultured.

17 of pneumococcal bacteremia despite negative subcultures.

18 roducible between amplifications and between subcultures.

19 clone, but caught up to it after four weekly subcultures.

20 y shorter than those for identification from subcultures.

21 at found in wild type R. monacensis after 15 subcultures.

22 e two Ureaplasma species in culture-positive subcultures.

23 een religious and nonreligious societies and subcultures.

24 at glucose broth or on sheep blood agar upon subculturing.

25 cells passed through stationary phase before subculturing.

26 side for 4 hours, and DNA was isolated after subculturing.

27 on to wild-type C. parasitica and successive subculturing.

28 e adipogenesis and replication declined with subculturing.

29 the same cultures 1 day later and from fresh subcultures 2 months later.

30 he exon 2 + 3 deletion assessed at 6 days of subculture after 4 hours of 0, 0.25, 1, 2.5, 5, and 10 m

31 lture total mouse keratinocytes for up to 19 subcultures, allowing an increase in cell number of grea

32 isolates for 12 months without the need for subculture and confirmed the viability of all isolates b

33 of contamination due to the needs of routine subculture and dark field microscopy.

34 to be equally comfortable in their own peer subculture and not to be different in the proportion tha

35 wn about which integrins are involved during subculture and passage.

36 After treatment, the cells were subcultured and grown for 7 days in medium without [125I

37 sitive blot; four unidentified colonies were subcultured and serotyped by the Quellung reaction.

38 ation from blood and following 5, 10, and 20 subcultures and at 1, 3, 6, and 12 months of storage at

39 transiently express exogenous GFP up to six subcultures and for at least 2 mo after infection, witho

40 ingle 0.1-ml sample from each clear tube for subculture, and adopting an alternate method for calcula

41 Bone tumors were subcultured, and chromosomal analysis demonstrated that

42 All fermenting specimens were subcultured, and isolates were tested for toxigenicity.

43 plated on CHROMagar Candida and RPMI medium, subcultured, and submitted for antifungal susceptibility

44 were recovered from Lowenstein-Jensen (BBL) subcultures, and 50 of the isolates were recovered from

45 hown that NO donors inhibit the migration of subcultured aortic smooth muscle cells.

46 Two of four isolates subcultured approximately 20 times ( approximately 500 c

47 At another 7 days, the growth in subculture at each time point was graded "1" for growth

48 ty of these age-related properties by serial subculture at low density of the two uncloned cultures a

49 d an acridine orange stain on day 8 and were subcultured at 2, 4, and 8 weeks.

50 Anaerobic BACTEC bottles were subcultured at 4 weeks.

51 ared as early as 4 weeks in culture and were subcultured at 8 weeks.

52 confluence, cells in KSFM were continuously subcultured at a 1-to-3 split.

53 pairment of proliferation when the cells are subcultured at low density and a greatly increased proba

54 made to results from prospective testing of subcultures at the Scottish MRSA Reference Laboratory, u

55 ase activity were generated by using freshly subcultured bacteria or by treating repeatedly subcultur

56 ee colonies) thereby preventing the need for subculturing bacterial isolates to reach a larger amount

57 Spirochetes could not be successfully subcultured by day 3 after exposure to ceftriaxone.

58 Cells were subcultured by transferring spheres to new culture dishe

59 nonliving macromolecules and transferred on "subculture" by self-propagating microcrystalline apatite

60 Furthermore, phospho-ERK levels in subcultured cells from ARF6(GTP) and ARF6(GDP) tumor exp

61 d not alter basal or stimulated migration of subcultured cells, except at a relatively high concentra

62 ects on cell proliferation in primary versus subcultured cells, indicating fundamental differences am

63 re and contrast their response with those in subcultured cells.

64 s telomerase-deficient mutants were serially subcultured, cells exhibited a progressive decline in av

65 Subcultured colonies were identified by 16S rRNA gene se

66 on-related genes were upregulated in stunted subcultures compared with the Fgmcm1 mutant, which was d

67 ng spectral profiles generated under various subculture conditions, as well as with and without hVISA

68 Stimulation of subcultured CSMC with UTP, ITP, or ATP induced a concent

69 emperatures, growth medium types, and repeat subcultures did not result in misidentification.

70 This assay does not require subculturing, DNA purification, restriction digestion, S

71 The ability of the WASP to subculture enrichment broths was evaluated with 106 Lim

72 enotype was stably maintained after multiple subcultures even in the absence of antibiotic selection.

73 Importantly, the cell line is continuously subcultured every 10-14 days when split at a 1:3 ratio a

74 Serial subcultures every 4 to 5 days were required to maintain

75 Initial subculture failed, but the organism was identified as He

76 1 mutants were unstable and produced stunted subcultures, Fgmcm1 mat1-1-1 but not Fgmcm1 fst12 double

77 ong stimulators of collagenase expression in subcultured fibroblasts of all types, including those fr

78 eal myofibroblasts (PCMs) were obtained from subcultured fibroblasts plated at a low density (5 cells

79 hed MS culture, incubated overnight and then subcultured for an additional 24 h, the agreement with X

80 Unidentified isolates were subcultured for repeat testing; 71/319 (22.3%) remained

81 On confluence, cells on AM were continuously subcultured for six passages on AM or plastic.

82 entry did not require routine entry or exit subcultures for either system.

83 sufficient proliferative activity to permit subculturing for at least 2 passages.

84 t Staphylococcus aureus (MRSA) directly upon subculture from positive blood culture bottles.

85 Unstimulated microglial cells, subcultured from an astrocyte coculture, typically exhib

86 in use, two (the E (Edinburgh) and J (Japan, subcultured from E)) are readily expelled by C57BL/6 mic

87 ation of the retinoblastoma protein in cells subcultured from the 60-day confluent control, AdBgl II-

88 to five different colonial morphologies were subcultured from the doxycycline medium, identified to s

89 We report that subcultures from lines of female human embryonic stem ce

90 ioral characteristic detected in low density subcultures from the confluent cultures, and it persists

91 isolates of Salmonella serovar Typhi, before subculture, from other regions where Vi-negative Salmone

92 ) in 206 LIM enrichment broths by the use of subculture, GBS peptide nucleic acid fluorescent in situ

93 The improved growth after subculture greatly enhanced the reliability of limit-dil

94 Consistent data were obtained from subcultures grown for 3-day and 6-day periods, from the

95 Viability of B. burgdorferi was assessed by subculture, growth, morphology, and pH (as a surrogate f

96 bcultured bacteria or by treating repeatedly subcultured H. pylori with flurofamide (1 microM), a pot

97 ous SOX9 mRNA can be rapidly up-regulated in subcultured human articular chondrocytes if grown in alg

98 BRL 49653 treatment of subcultured human pre-adipocytes prepared from all depot

99 A representative of each was subcultured, identified to genus and species level, and

100 for toxin-producing E. coli O157:H7, and IMS subculture improved recovery.

101 n these cells were allowed to proliferate by subculture in DPI-free medium, an extensive G(1) delay w

102 ed at a much slower rate than controls after subculture in the absence of the drug, and required 9-12

103 lf-harm is associated with contemporary goth subculture in young people; however, whether this associ

104 y with full recovery of urease activity when subcultured in fresh microaerobic broth medium.

105 lysis of transcripts from human lymphoblasts subcultured in lipid-depleted sera (LDS) and LDS supplem

106 our hypothesis, S. flexneri strain 2457T was subcultured in media containing bile salts, and the abil

107 orbol myristate acetate or were passaged and subcultured in monolayer to induce dedifferentiation.

108 After initial pilot studies, they were subcultured in one of three groups: 1% porcine serum plu

109 When both sexual behaviors exist as subcultures in a population, disruptive selection can re

110 r transported in BHI followed by plating and subculturing in an enrichment medium, provides a simple

111 Frequent subcultures increased the number of unidentified isolate

112 ed their differential expression in parallel subcultures incubated with and without UVA.

113 epitope retain this level of expression when subcultured into broth.

114 ontent could be obtained in under 6 h from a subcultured isolate, less time than traditional phenotyp

115 Subcultures made in the presence of FGF-4 had up to 10-f

116 opoiesis for several months, while untreated subcultures, made without FGF-4, grew erratically and ge

117 n the high rate of false negatives, terminal subcultures may be helpful in certain situations.

118 atories have shown that during isolation and subculturing mesenchymal stem cells quickly lose the exp

119 gest that young people identifying with goth subculture might be at an increased risk for depression

120 is population by using a chromogenic medium, subculturing, molecular typing, and antifungal susceptib

121 Haploid yeast tel1 mec1 strains were subcultured nonselectively for approximately 200 cell di

122 Terminal subculture of "negative" bottles demonstrated viable yea

123 s, all BacT aerobic bottles, and by terminal subculture of all bottles.

124 analytical accuracy in comparison to routine subculture of blood culture bottles and phenotypic ident

125 Terminal subculture of bottles without detected growth recovered

126 visualization of pigment on day 1 or from a subculture of carrot broth.

127 included Gram staining of growth on BEAA and subculture of cocci on sheep blood agar plates for vanco

128 A subculture of students at each university form social bo

129 aecalis, and 1 Enterobacter sp.) on terminal subculture of the AER bottles when the companion PEDS PL

130 pported by exogenous FGF-2 and eliminated by subculture of the cells in presence of serum.

131 In addition, a subculture of the isolate was tested by a microdilution

132 the cell cycle-inhibitory proteins following subculture of the LTC cultures.

133 -negative cultures were detected by terminal subculture of the PEDS PLUS bottles when the companion A

134 On detailed examination, subcultures of 25 of the 32 VRE isolates produced two di

135 It also occurs in serial subcultures of cells that had been held under the constr

136 at XCI status should be routinely checked in subcultures of female hESCs, with cultures displaying XC

137 ion of BMPR expression was also evaluated in subcultures of human pulmonary arteriolar endothelial ce

138 ed on both media, and 12 were recovered from subcultures of SBM only.

139 ry isolation plate without the need for pure subcultures of suspect bacteria.

140 Incubation temperatures and subcultures of the media did not alter the rate of ident

141 Subcultures of the NSFCs have been passaged nearly 200 t

142 Repeated subculturing of AB22 resulted in improved growth and los

143 real-time PCR detection following successive subculturing of the bacterial isolate.

144 Repeated subculturing of Xanthobacter strain Py2 under nonselecti

145 ing modified 7H9 broth (8 weeks) followed by subculture on HEYM slants (monitored up to 8 weeks), and

146 specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick agar.

147 nantly gram-positive cocci in clusters) were subcultured on 5% sheep blood agar plates.

148 Primary rabbit endothelial cells were subcultured on 96-well plates at densities ranging from

149 ted to be senescent, since they could not be subcultured on agar medium.

150 tic morphology and keratocan expression when subcultured on AM stromal matrix even in the presence of

151 plastic was downregulated without CD34 when subcultured on AM.

152 agenase-isolated keratocytes were seeded and subcultured on plastic or amniotic membrane matrix (AM)

153 A expression was upregulated when cells were subcultured on plastic.

154 d negative, the Gram stain was negative, and subcultures on chocolate agar and from the Isolator tube

155 and subsequent Gram stains were positive and subcultures on chocolate agar grew bacteria.

156 Subcultures on Lowenstein-Jensen agar confirmed the viab

157 Through serial-subcultures on xylose, we isolated evolved strains which

158 re incubated an additional 24 h for a second subculture, only 1 of 224 tests (0.4%) remained discrepa

159 Primary subculture onto Middlebrook 7H10, however, revealed thre

160 At 2 weeks, all plates were subcultured onto a fresh medium.

161 SBM cultures were subcultured onto blood agar and CNA agar plates, and the

162 ml, aliquots of catheter-exposed broth were subcultured onto blood agar at 15-min intervals.

163 h of the enrichment techniques, samples were subcultured onto modified semisolid Rappaport-Vassiliadi

164 Yeast isolates were subcultured onto Sabouraud dextrose agar and were incuba

165 Fecal specimens are subcultured onto selective and nonselective media, inclu

166 le functional properties following extensive subculture or differentiation into myofibroblasts and re

167 nge in MICs was noted following 5, 10, or 20 subcultures or at up to 6 months of frozen storage.

168 ce was stable in vitro after three rounds of subculture over 48 hours of growth in the absence of ant

169 (128 to 173 x 10(-8)) after 2 and 6 days of subculture (P < .001 overall).

170 stable in 10 strains following 10 sequential subculture passages.

171 -positive colonies from the direct and broth subculture plates were evaluated for the presence of VRE

172 24 tests (92%) were reproducible at the 24-h subculture point (94% for the SBT assay and 91% for the

173 c marker for BAT, in isolated adipocytes and subcultured preadipocytes prepared from different adult

174 associated antigen were observed in older or subcultured preparations concomitantly with the appearan

175 pB was associated with the outer membrane in subcultured preparations of H. pylori.

176 Using a recently described subculturing protocol to "induce" or accelerate EPEC adh

177 in neurofibroma formation and, by selective subculture, provide a resource for the development of an

178 ability within a laboratory occurred between subcultures rather than within gels or between gels.

179 These strains could be subcultured repeatedly and retained capacity for differe

180 m the cultures after growth of 5, 10, and 15 subcultures, respectively.

181 Serial subculture resulted in a gradual increase in growth rate

182 These subcultures retained the capacity to support hematopoies

183 colonies appearing on selective plates were subcultured, serotyped by the Quellung reaction, and gen

184 Upon subculture, stem cells formed colonies until passage 6 a

185 The subcultured strains had very high rates of chromosome ab

186 f freshly isolated corneal stromal cells and subcultured stromal fibroblasts from rabbits was used fo

187 etent to activate NF-kappaB in comparison to subcultured stromal fibroblasts.

188 act agar during the initial isolation and/or subcultures than they did on sheep blood or chocolate ag

189 lts between PCR and culture were resolved by subculturing the enrichment broth.

190 However, upon extensive subculturing, the level of exotoxin A produced by the PA

191 y difficult to grow as primary cultures, and subculturing these cells has been virtually impossible u

192 When subcultured, they began to grow and showed increased upt

193 crograms of colistin per ml (LIM broth) with subculture to another BAP and the costs associated with

194 nada agar to a Todd-Hewitt broth method with subculture to blood agar in order to determine which GBS

195 there was no growth of bacteria or yeasts on subculture to chocolate agar.

196 Carrot broth-enhanced subculture to GBS Detect (Hardy Diagnostics, Santa Maria

197 ple, the use of broth enrichment followed by subculture to MS offered a low-cost but sensitive method

198 without broth enrichment followed by a 24-h subculture to MS, was performed.

199 RSA (CM) and overnight broth enrichment with subculture to MSA (broth).

200 ctum in a selective broth medium followed by subculture to solid media and identification of GBS on t

201 broth containing 1 mg/liter of meropenem and subcultured to a MacConkey agar plate with a 10-mug mero

202 After 1 to 2 days of incubation, broths were subcultured to BEA VAN6 microg/ml agar.

203 ) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted f

204 ks, MD) positive by gram stain for yeast was subcultured to CHROMagar Candida (BD), and a 25-microg f

205 les flagged positive by the instruments were subcultured to determine both true-positive (growth) and

206 In addition, the same species were subcultured to fresh blood plates daily and DNA was extr

207 The differentiating culture can be subcultured to produce large amounts of myogenic progeni

208 sor mutations occurred frequently in stunted subcultures to recover growth rate.

209 tially associate with others who share their subculture, tool-using dolphins prefer others like thems

210 onditioned medium that allows us to grow and subculture total mouse keratinocytes for up to 19 subcul

211 mitans JP2-12 grew to high cell density when subcultured under iron-replete conditions.

212 ') appear in approximately 4 weeks; they are subcultured until adequate material is obtained for anal

213 DeltaNp63alpha or empty vector and serially subcultured until replicative senescence.

214 d eight plexiform neurofibromas by selective subculture using glial growth factor-2 and laminin.

215 dal concentrations (MFCs) were determined by subculturing visibly clear wells from the MIC microtiter

216 amined the effects loss-of-PDE1A function on subcultured VSMC growth and survival using PDE1A RNA int

217 We further demonstrated that in subcultured VSMCs redifferentiated by growth on collagen

218 , to assess false-negative bottles, terminal subcultures were done on all negative companion bottles

219 (MCF10A) with C/EBPbeta-2 virus, transformed subcultures were readily generated.

220 All solid media, including subcultures, were incubated for 8 weeks, providing a tot

221 akes at least 24 h to complete after isolate subculture, whereas hVISA is not routinely detected in c

222 l fast disease in mice, even after extensive subculture, whereas SY-infected cells produced only slow

223 ance, particularly if based on single-colony subcultures, will likely underestimate transmission even

224 Following sorting and subculture with MSCs, this CD34(+)CD41(low) population w

225 KSPG secretion was lost during serial subculture with or without FGF-2.

226 By repeated subculturing with increasing vancomycin (VAN) and cefuro

227 f the drug, and required 9-12 days of serial subculture, with selective growth of the faster growing

228 Gram stain can impart, and in less time than subculturing, would allow the use of more directed empir

229 In summary, subculturing yeast directly from blood cultures onto CHR

230 cterium bovis that was isolated after serial subcultures, yet the functional basis for this attenuati