ライフサイエンスコーパス: subgenomic

1 from making less to making more genomes than subgenomic 26S mRNA at late times during infections.

2 Subgenomic analysis of the HCV core gene indicated that

3 ore, IFN-lambda blocked the replication of a subgenomic and a full-length genomic HCV replicon in hum

4 the difference in CyPA dependence between a subgenomic and a full-length replicon of JFH-1 was due,

5 Also, using hepatoma cells that harbor subgenomic and full-length replicons of HCV, we found th

6 n HCV genotype 1b, the effects of psh-274 on subgenomic and full-length replicons were examined, and

7 ng Stem 3 modulate -1 PRF and play a role in subgenomic and full-length RNA synthesis.

8 In subgenomic and genomic analyses of subcellular mRNA part

9 ma) has been shown to inhibit replication of subgenomic and genomic hepatitis C virus (HCV) RNAs in v

10 vely with HCV RNA levels in four independent subgenomic and genomic replicon lines (R = 0.41, P = 0.0

11 Thus, BMV subgenomic and genomic RNA syntheses mutually interfered

12 early and late protein expression as well as subgenomic and genomic RNA transcription, were diminishe

13 t concentrations were observed with both the subgenomic and JFH1-derived infectious HCV models.

14 atoma cells expressing HCV (full-length, BB7-subgenomic, and JFH-1 clone) compared with control HCV-n

15 d the accumulation of protein A and genomic, subgenomic, and template viral RNA.

16 recalcitrant CCV genome as three overlapping subgenomic bacterial artificial chromosomes (BACs).

17 ar mRNAs, reinitiation occurs efficiently on subgenomic bicistronic calicivirus mRNAs, enabling synth

18 imately 5-fold less efficient than that from subgenomic CAT mRNA derived from an IGR-containing MG, b

19 virions, whereas genomic RNA3 (gB3) and its subgenomic coat protein (CP) mRNA (sgB4) are copackaged

20 Full-length HCV and HCV subgenomic constructs corresponding to structural and ea

21 truncated capsid proteins generated from HEV-subgenomic constructs that represent all four viral geno

22 rotoplasts to examine the recognition of the subgenomic core promoter by the BMV replicase.

23 recognition of the brome mosaic virus (BMV) subgenomic core promoter by the replicase.

24 entical to those in BMV, suggesting that the subgenomic core promoter can induce the BMV replicase in

25 lts indicate that key nucleotides in the BMV subgenomic core promoter direct replicase recognition bu

26 trand RNA synthesis could substitute for the subgenomic core promoter in transfected cells.

27 dditional functional characterization of the subgenomic core promoter was performed.

28 rus (CCMV) could also substitute for the BMV subgenomic core promoter.

29 enotype 1a) cDNA constructs (full-length and subgenomic), core protein alone, viral RNA, or replicati

30 Thus, analysis of population structure with subgenomic data shows the distinction of hospital and no

31 ted with shorter genomic segments as well as subgenomic DI particles.

32 at pH 7.2 and 37 degrees C in particles with subgenomic DNA, suggesting that pressure exerted by the

33 ed by the accumulation of virions containing subgenomic DNAs of specific sizes.

34 le L172T, the most impaired mutant, had long subgenomic DNAs originating from both termini, suggestin

35 During infection, pathogenic subgenomic flaviviral RNAs (sfRNAs) are produced by resi

36 Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to p

37 clease Xrn1 for the production of pathogenic subgenomic flaviviral RNAs.

38 Flaviviruses produce an abundant noncoding subgenomic flavivirus RNA (sfRNA) in infected cells.

39 he PR-2B DENV-2 produced increased levels of subgenomic flavivirus RNA (sfRNA) relative to genomic RN

40 The DENV non-structural protein NS4B and subgenomic flavivirus RNA interfere with the RNA interfe

41 Moreover, tick-borne flaviviruses expressed subgenomic flavivirus RNAs that interfere with tick RNAi

42 By sequencing a subgenomic fragment of the HIV-1 envelope from study par

43 Subgenomic fragments comprising a complete provirus were

44 for the genetic subtyping of full-length and subgenomic fragments of HIV-1.

45 eichenowi and Plasmodium gaboni based on the subgenomic fragments.

46 removed both miR-122 binding sites from the subgenomic GBV-B RNAs rendered viral RNA amplification i

47 7 cells supporting autonomous replication of subgenomic GBV-B RNAs.

48 nd to block subgenomic genotype 1b (Luc-1b), subgenomic genotype 1a (Luc-1a), and genomic genotype 2a

49 c genotype 1b clones (SG-1b and Luc-1b), two subgenomic genotype 1a clones (SG-1a and Luc-1a), JFH-1

50 olecule inhibitor, PIK93, was found to block subgenomic genotype 1b (Luc-1b), subgenomic genotype 1a

51 replication and/or HCV RNA levels of the two subgenomic genotype 1b clones (SG-1b and Luc-1b), two su

52 required for HCV replication, we screened a subgenomic genotype 1b replicon cell line (Luc-1b) with

53 equence of 3D(pol), ablated replication of a subgenomic HAV replicon in transfected human hepatoma ce

54 1 and 2'-C-methylcytidine were assayed in a subgenomic HCV replicon assay system and found to be pot

55 unwinding >50% at 2 +/- 1 muM, inhibited the subgenomic HCV replicon at 10 muM, and was not toxic at

56 biosynthesis regulate the replication of the subgenomic HCV replicon in Huh-7 cells.

57 f hepatitis C virus (HCV) replication in the subgenomic HCV replicon system, and its corresponding 5'

58 viral replication capacity, as assessed in a subgenomic HCV replicon system.

59 tic activity and inhibits the ability of the subgenomic HCV replicon to replicate in Huh-7 cells.

60 3A hydrophobic domain, in the context of the subgenomic HCV replicon, was stably maintained throughou

61 evaluated by site-directed mutagenesis of a subgenomic HCV replicon.

62 epatoma cells that express luciferase-tagged subgenomic HCV replicons (Huh7/HCV replicon cells) or th

63 Moreover, HCV infection or subgenomic HCV replicons produced a dramatic increase in

64 pathway in mammals, as has been reported for subgenomic HCV replicons, siRNAs that target Dicer inhib

65 and all but NF 023 inhibited replication of subgenomic HCV replicons.

66 shed light on the intracellular dynamics of subgenomic HCV RNA replication from transfection to stea

67 Additionally, the subgenomic HCV RNA was found to replicate more efficient

68 DDX6 abundance influenced the replication of subgenomic HCV RNAs lacking core sequence, the relevance

69 mpaired or completely ablated the ability of subgenomic HCV RNAs to induce cell colony formation.

70 tion, as well as by transient replication of subgenomic HCV RNAs.

71 otein-encoded defects in HCV by constructing subgenomic HCV transcripts capable of simultaneously exp

72 we directly characterized ex vivo total and subgenomic HCV-specific CD4(+) and CD8(+) T cell respons

73 Huh-7.5 cells harboring subgenomic hepatitis C virus (HCV) replicons or infected

74 is study, we show that replication-competent subgenomic hepatitis C virus (HCV) RNA can be transferre

75 cell-free P3HR-1 virion DNA, confirming that subgenomic het DNA was packaged into infectious particle

76 ant to alpha interferon (IFN-alpha) therapy, subgenomic in vitro self-replicating HCV RNAs (HCV repli

77 tein-Barr virus (EBV) strain P3HR-1 generate subgenomic infectious particles that, unlike defective i

78 Key nucleotides required for subgenomic initiation in vitro were found to be importan

79 used to determine the cellular diversity of subgenomic JFH-1 HCV replicons constitutively expressed

80 We analyzed replication of the subgenomic JFH1 replicon in embryonic fibroblasts and pr

81 In WMoV-infected wheat, subgenomic-length mRNAs of vc sense were detected for ge

82 t and provided evidence for the synthesis of subgenomic-length mRNAs of virus complementary sense.

83 ort to demonstrate that emaraviruses produce subgenomic-length mRNAs that are most likely utilized fo

84 Massively parallel sequencing of captured subgenomic libraries interrogated 99.8% of targeted nucl

85 foci at chromosomal DSBs and pinpoints their subgenomic locations.

86 replication, we designed and characterized a subgenomic luciferase reporter construct.

87 led to diminished expression from alphavirus subgenomic messages, whereas no dramatic diminution in c

88 Previous studies indicated that a 719-nt subgenomic minigenome (DENV-MINI) is an efficient templa

89 complex multigenomic DNA molecules to simple subgenomic molecules.

90 Coronavirus subgenomic mRNA (sgmRNA) synthesis occurs via a process

91 ruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription.

92 nstructural protein that is expressed from a subgenomic mRNA in the cytoplasm of virus-infected cells

93 g from these switches were not templates for subgenomic mRNA synthesis but, rather, ambisense chimera

94 can be used for production of templates for subgenomic mRNA synthesis from the DI RNA.

95 r-containing templates used subsequently for subgenomic mRNA synthesis.

96 tly as the wild type did but had a defect in subgenomic mRNA synthesis.

97 ity of leader-body junction formation during subgenomic mRNA synthesis.

98 trol translation, genomic RNA synthesis, and subgenomic mRNA transcription.

99 virus polymerase with equal efficiency, but subgenomic mRNA was undetectable.

100 he ambisense orientation expressed NSs via a subgenomic mRNA, and two viruses grew to titers only mod

101 bling expression of nsp1 from DI RNA-encoded subgenomic mRNA, DI RNA levels were greatly reduced, but

102 RNA and the transcription and translation of subgenomic mRNA.

103 nd in the otherwise-identical nonreplicating subgenomic mRNA7 (sgmRNA7).

104 uctural protein ORFs from a nested set of 3' subgenomic mRNAs (sg mRNAs), and for most of these ORFs,

105 , to place a 5'-terminal genomic leader onto subgenomic mRNAs (sgmRNAs).

106 ockdown of DHX9 increased the ratio of short subgenomic mRNAs (sgmRNAs); in contrast, DHX9 overexpres

107 It is unclear how viral genomic and subgenomic mRNAs compete with each other for the cellula

108 licate through the use of a 3' nested set of subgenomic mRNAs each possessing a leader (65 to 90 nucl

109 ve-strand RNA viruses generate 3'-coterminal subgenomic mRNAs to allow translation of 5'-distal open

110 nonnative locations, mostly late-transcribed subgenomic mRNAs, in the presence or absence of the nati

111 ptional termination of two S-segment-derived subgenomic mRNAs, which revealed that transcription term

112 and their structural proteins are encoded by subgenomic mRNAs.

113 ails genome replication and transcription of subgenomic mRNAs.

114 es a template switch during the synthesis of subgenomic negative-strand RNAs to add a copy of the lea

115 sense of genomic RNAs are expressed through subgenomic- or near-genomic-length vc-sense mRNAs.

116 nduced HPV16 late gene expression from HPV16 subgenomic plasmids and from episomal forms of the full-

117 cytidylate in the templates for genomic and subgenomic plus-strand RNA synthesis significantly decre

118 rum of genome/mRNA cleavage because only the subgenomic pol III transcripts are efficiently processed

119 ecent in vivo studies have revealed that the subgenomic promoter (sgp) in brome mosaic bromovirus (BM

120 homologous recombination activity within the subgenomic promoter (sgp) region in brome mosaic virus (

121 at the specific recognition of the norovirus subgenomic promoter is through binding by the viral RdRp

122 inding sequence (TABS) of the capsid protein subgenomic promoter on RNA-1 and trans-activates subgeno

123 Old World alphaviruses using a second viral subgenomic promoter to express the transgenes, placed ei

124 compared these with a traditional 3' double subgenomic promoter virus expressing either a large, fir

125 he stem-loop forms the core of the norovirus subgenomic promoter.

126 s-strand initiation, and one was in the core subgenomic promoter.

127 Huh-7 cells or Huh-7 stably replicating HCV subgenomic protein core through nonstructural protein 3

128 l culture and encode a unique protein in the subgenomic region designated as leader of the capsid pro

129 ickens, bats, and rodents in three conserved subgenomic regions (residues 1 to 452 or 974 to 1534 of

130 ng a panel of canine BAC clones representing subgenomic regions of particular interest.

131 he analysis of RSV full genomes, compared to subgenomic regions, provided more precise estimates of t

132 enes to identify genes required for both HCV subgenomic replication and infectious virus production.

133 ated with inhibition of HCV replication in a subgenomic replication system for a series of non-nucleo

134 e induction of hepatic MRP2 secondary to HCV subgenomic replication.

135 human hepatoma cell line expressing the HCV subgenomic replicon (Huh.8) to evaluate CYP1A1 induction

136 Therefore, we measured genotype 1b subgenomic replicon (sg1b) RNA levels under various IFN-

137 ys that included a luciferase expressing WNV subgenomic replicon and an NS1 capture enzyme-linked imm

138 gged NS5A from cells harboring a replicating subgenomic replicon and analyzed the purified protein by

139 series of mutations into the NS5B of an HCV subgenomic replicon and examined the replication capabil

140 through site-directed mutagenesis of a Con1 subgenomic replicon and through biophysical characteriza

141 we synthesized and transcribed a genotype 4a subgenomic replicon and transfected Huh7-Lunet cells wit

142 ed and showed significant potency in the HCV subgenomic replicon assay (<1 muM) and produced high lev

143 ty in the cell-based hepatitis C virus (HCV) subgenomic replicon assay, by virtue of intracellular co

144 ificant antiviral activity in a dengue virus subgenomic replicon assay.

145 s 1a and 1b polymerase inhibition assays and subgenomic replicon assays.

146 ited more potent anti-HCV activity in an HCV subgenomic replicon cell based assay (EC90 = 1.9, 7.4, a

147 tantly, the same events are triggered by HCV subgenomic replicon cells but not by free virus particle

148 We report that a Con1 chimeric subgenomic replicon containing the NS4B gene from the cl

149 This ability was also shared by a subgenomic replicon derived from the related GB virus B

150 eover, a full-length HCV replicon, but not a subgenomic replicon devoid of the core gene, significant

151 Using the HCV subgenomic replicon expression system, we show that secr

152 he development of a selectable, bi-cistronic subgenomic replicon for bovine viral diarrhea virus (BVD

153 we demonstrate that the expression of an HCV subgenomic replicon in cultured cells results in the acq

154 VI) are essential for replication of the HCV subgenomic replicon in Huh-7 cells.

155 es in NS5A supported stable replication of a subgenomic replicon in Huh-7.

156 atitis C virus (HCV) genotype 1b strain Con1 subgenomic replicon in human hepatoma cells.

157 sence of other nonstructural proteins of the subgenomic replicon is in part responsible.

158 However, the mutant form of a subgenomic replicon of genotype 3 HEV replicated more ef

159 that are essential for accumulation of GBV-B subgenomic replicon RNA.

160 s-packaging system for West Nile virus (WNV) subgenomic replicon RNAs (repRNAs), deleted for the stru

161 es on individual NS4B proteins and on an HCV subgenomic replicon showed that the lipid modifications,

162 ific inhibitor of HCV replication in the HCV subgenomic replicon system (IC(50) = 1.28 microM) with s

163 We used a subgenomic replicon system to assess the effects of the

164 describe in vitro resistance studies using a subgenomic replicon system to compare VX-950 with anothe

165 inhibit HCV RNA replication in a cell-based, subgenomic replicon system, while nucleoside triphosphat

166 an IC(50) of 0.17 microM in the genotype 1b subgenomic replicon system.

167 tion of hepatitis E virus genotype 3 both in subgenomic replicon systems as well as a full-length inf

168 ase region of a hepatitis C virus (HCV) Con1 subgenomic replicon to ascertain the role of the helicas

169 thermore, when Huh-7 cells harboring the HCV subgenomic replicon were treated with a synthetic peptid

170 in Huh7-derived EN5-3 cells harboring an HCV subgenomic replicon with the nonstructural region NS3-NS

171 ontext of the cells transfected with the HCV subgenomic replicon, all except one prevented colony for

172 rface and subsequently examined using an HCV subgenomic replicon, resulting in significant reduction

173 Using a genotype 1b subgenomic replicon, we determined the effect of mutatio

174 The JFH-1 subgenomic replicon, which replicated to high levels in

175 omolar potency against the NS3 protease in a subgenomic replicon-based cellular assay (Huh-7).

176 However, growth of the subgenomic replicon-containing Huh-7.5 cells could be st

177 processing was examined in the context of a subgenomic replicon.

178 re observed with both viral infections and a subgenomic replicon.

179 dramatically decreases replication of an HEV subgenomic replicon.

180 markers in the context of the full-length or subgenomic replicon.

181 ed NS5A in Huh-7 cells stably expressing the subgenomic replicon.

182 ut not essential for colony formation by the subgenomic replicon.

183 culture infectious virus and a corresponding subgenomic replicon.

184 ple hydroxamic acids for inhibition of a HCV subgenomic replicon.

185 anti-HCV activities using genotype 2a JFH-1 subgenomic replicons and infectious virus systems.

186 ure, finally leading to the establishment of subgenomic replicons and the infectious virus model (HCV

187 Chimeric H77 subgenomic replicons containing the entire NS4B gene fro

188 Furthermore, both genotype 1b and 2a subgenomic replicons expressing nonstructural (NS3-5B) p

189 analyzed the impact of SIL on replication of subgenomic replicons from different HCV genotypes in vit

190 udies of drug-selected, cell culture-adapted subgenomic replicons have indicated that an RNA element

191 DNA) library, transfected the cells with HCV subgenomic replicons lacking adaptive mutations, and sel

192 of RNA replication, which was observed using subgenomic replicons of two different genotypes.

193 small interfering RNA in cells carrying HCV subgenomic replicons severely reduced viral replication,

194 ng HCV genotype 1a, 1b, or 2a full-length or subgenomic replicons were resistant to infection with ce

195 permissive Huh7 cells and three independent subgenomic replicons with various replicative capacities

196 loped to simultaneously select different HCV subgenomic replicons within the same cell.

197 expression is elevated in cells carrying HCV subgenomic replicons, but XBP1 trans-activating activity

198 Even with subgenomic replicons, lacking structural viral proteins,

199 tease domain proteins and in genotype 1b HCV subgenomic replicons.

200 by phenotypic screening campaigns using HCV subgenomic replicons.

201 ifferent human liver-derived cell lines with subgenomic reporter replicons of HAV as well as of diffe

202 Using subgenomic reporters, as well as HIV replication assays,

203 ed the levels of RNA synthesis directed from subgenomic ribonucleoprotein (RNP) templates.

204 icistronic open reading frame encoded within subgenomic RNA 7.

205 witching during negative-strand synthesis of subgenomic RNA 7.

206 proteins, but in murine norovirus (MNV), the subgenomic RNA also encodes the VF1 protein, which funct

207 otential cis-acting elements at the start of subgenomic RNA and the 3' end of NV genome for the virus

208 While a West Nile virus (WNV) subgenomic RNA could readily be packaged by structural p

209 he Caliciviridae family of viruses produce a subgenomic RNA during infection.

210 transcription of pregenomic RNA (pgRNA) and subgenomic RNA from the HBV covalently closed circular D

211 Although a subgenomic RNA has been detected in TV-transfected cells

212 ed 6 nucleotides 3' of the start site of the subgenomic RNA in all caliciviruses.

213 etics and affected the levels of genomic and subgenomic RNA in infected cells.

214 that functions as the core of the norovirus subgenomic RNA promoter in cells and in vitro.

215 s also found to be a potent inhibitor of HCV subgenomic RNA replication with IC(50) and IC(90) of 40

216 n addition to being potent inhibitors of HCV subgenomic RNA replication, some of the new P(4)-capped

217 ROS on HCV replication, using a bicistronic subgenomic RNA replicon and a genomic RNA that can repli

218 uh7 hepatoma cell line that contained an HCV subgenomic RNA replicon and also expressed a GFP-LC3 fus

219 In this report, we demonstrate that a subgenomic RNA replicon of genotype 2a HCV replicated ef

220 rboring different variants of an HCV type 1b subgenomic RNA replicon that differed at only two sites

221 autophagosomes from cells that harbor an HCV subgenomic RNA replicon.

222 The recent development of HCV subgenomic RNA replicons has permitted the study of vira

223 and demonstrating that BMV can give rise to subgenomic RNA replicons.

224 d virions, despite the great molar excess of subgenomic RNA species that is present in infected cells

225 The full-length genomic and subgenomic RNA strands were detected by Northern blottin

226 otein NTD plays a critical accessory role in subgenomic RNA synthesis and other processes requiring R

227 To date, the mechanism of norovirus subgenomic RNA synthesis has not been characterized.

228 r plastic, structural element in stimulating subgenomic RNA synthesis in coronaviruses.

229 -190 in a way that is critical for efficient subgenomic RNA synthesis in MHV.

230 nserved hexanucleotide sequence required for subgenomic RNA synthesis.

231 99 to N corrected the D41A-induced defect in subgenomic RNA synthesis.

232 SL1 and SL2 are required for subgenomic RNA synthesis.

233 tion may play an important role in directing subgenomic RNA synthesis.

234 ing this deletion are defective in directing subgenomic RNA synthesis.

235 y formed transcription intermediate in viral subgenomic RNA synthesis.

236 en the 5' UTR and the 3' UTR that stimulates subgenomic RNA synthesis.

237 mic RNA in addition to its known function in subgenomic RNA synthesis.

238 s at the extreme 3' end of the MHV genome in subgenomic RNA synthesis.

239 enomic promoter on RNA-1 and trans-activates subgenomic RNA synthesis.

240 e promoter and a short template sequence for subgenomic RNA synthesis.

241 ivities essential for coronavirus genomic or subgenomic RNA synthesis.

242 detected in TV-transfected cells, a separate subgenomic RNA transcript was not required for the initi

243 the BMV replicase in interactions needed for subgenomic RNA transcription in vivo.

244 The subgenomic RNA typically encodes only the major and mino

245 essed genomic RNA was found to replicate; NV subgenomic RNA was transcribed from genomic RNA by use o

246 gene expression, (ii) a new role for a viral subgenomic RNA, and (iii) a new mechanism for RNA-mediat

247 y into either a stable cell line replicating subgenomic RNA, or a transient full-length HCV replicati

248 This subgenomic RNA, which was capped, initiated at nucleotid

249 tected full-length replicon RNA and a single subgenomic RNA.

250 ion of viral genome and transcription of the subgenomic RNA.

251 results reveal (i) a new level of control of subgenomic-RNA gene expression, (ii) a new role for a vi

252 ey yellow dwarf virus genomic RNA (gRNA) and subgenomic RNA1 (sgRNA1) is driven by the powerful cap-i

253 A third protein, B2, is translated from a subgenomic RNA3 derived from the 3' end of RNA1.

254 A small subgenomic RNA3, which encodes nonstructural proteins B1

255 predicted to express from the second ORF in subgenomic RNA3.

256 The synthesis of 3' subgenomic RNA4 (sgRNA4) by initiation from an internal

257 tations in the third template nucleotide for subgenomic RNA4 resulted in accumulations at between 7 a

258 t the production of positive- or minus-sense subgenomic RNA7.

259 trast, bovine leukemia virus (BLV) expresses subgenomic RNAP III transcripts that give rise to miRNAs

260 tems consisting of pairs of self-replicating subgenomic RNAs (replicons) derived from tick-borne ence

261 replacement of structural genes, encoded by subgenomic RNAs (SG RNA), with heterologous sequences of

262 ble RdRp binding, accurate initiation of the subgenomic RNAs and efficient RNA synthesis.

263 ated lesser amounts of plus- and minus-sense subgenomic RNAs and spike protein than WT virus.

264 genomic RNA, which resulted in production of subgenomic RNAs as well.

265 nomic minus-strand, genomic plus-strand, and subgenomic RNAs in barley protoplasts transfected with w

266 mosaic virus (BMV) packages its genomic and subgenomic RNAs into three separate viral particles.

267 However, synthesis of viral genomic and subgenomic RNAs was severely suppressed by UO126 treatme

268 ffect but were competent to synthesize viral subgenomic RNAs, and 8 were not viable.

269 ntirely ablated packaged large quantities of subgenomic RNAs, in addition to genomic RNA.

270 ted in vivo in the absence of detectable TCV subgenomic RNAs, strongly suggesting that the IRES was a

271 tely positioned CS elements for synthesis of subgenomic RNAs.

272 transgene in the CNS, suggesting that the DA subgenomic segment can cause cellular dysfunction but no

273 has tamoxifen (Tm)-inducible expression of a subgenomic segment of DA RNA in oligodendrocytes and Sch

274 The full-genome and subgenomic sequences identified in our study population

275 n the infected hosts: (i) mediation of viral subgenomic (sg) mRNA transcription and (ii) suppression

276 a 3' coterminal nested set of five to eight subgenomic (sg) mRNAs are made that are also 5' cotermin

277 putative viral accessory protein encoded on subgenomic (sg) RNA 7.

278 -infected cells, the genomic (G) RNA and the subgenomic (SG) RNA.

279 and RNAs, a genomic-length RNA (G) RNA and a subgenomic (SG) RNA.

280 ositive-strand RNAs, a genomic (G) RNA and a subgenomic (SG) RNA.

281 riptase to the promoter for the synthesis of subgenomic (SG) RNA.

282 voiding packaging of the more abundant viral subgenomic (SG), cellular messenger and transfer RNAs in

283 Using a subgenomic short hair RNA library targeting approximatel

284 ol of virus infection by constructing double subgenomic Sindbis viruses that expressed the mISG15 R15

285 s of full-length anti-MG (aMG) replicate and subgenomic size mRNA for reporter gene expression.

286 a "master circle" chromosome and as numerous subgenomic sublimons that are generated by intramolecula

287 e-strand break in the whole genome and/or in subgenomic targets such as telomere sequences.

288 t capsid expression requires production of a subgenomic transcript, the presence of capsid often bein

289 xed regulatory circuits promoted inefficient subgenomic transcription from inappropriate start sites,

290 s virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstre

291 gy for DNA capture that uses PCR products as subgenomic traps.

292 A subgenomic Ty1 mRNA encodes a truncated Gag protein (p22

293 th type 2 JFH-1 HCV or transfection with the subgenomic type 1 HCV replicon.

294 of both plus-strand RNA species, genomic and subgenomic, varied widely, indicating that this region o

295 The 3' ends of subgenomic viral mRNAs have been mapped to a stem-loop s

296 e inoculated with SARS-CoV, and increases in subgenomic viral RNA from 1-16 h or more were detected b

297 sequentially altered the ratio of genomic to subgenomic viral RNA synthesis, promoted recovery of cel

298 sequencing approach, we characterize several subgenomic viral RNAs from human nasopharyngeal specimen

299 rols IFN resistance in HeLa cells expressing subgenomic WNV replicons lacking the structural genes.

300 s verified through a de novo RdRp assay on a subgenomic ZIKV RNA template.