ライフサイエンスコーパス: substrate binding

1 motions were not significantly altered upon substrate binding.

2 ace and cap domain, priming one protomer for substrate binding.

3 an open conformation to initiate productive substrate binding.

4 y affect residue 132, which helps coordinate substrate binding.

5 at leads to the active site, thus preventing substrate binding.

6 e site and provides additional restraints on substrate binding.

7 eduction of the active-site copper initiates substrate binding.

8 e indicated a functional role of His(163) in substrate binding.

9 "lid" to close off the active site following substrate binding.

10 this position is essential for high-affinity substrate binding.

11 RS isoforms may result from altered modes of substrate binding.

12 r with spatially well-separated cavities for substrate binding.

13 d the spectrophotometric characterization of substrate binding.

14 wed spectral blue shifts of CYP2J2 following substrate binding.

15 tion and primes the AAA ATPase cassettes for substrate binding.

16 fide bond shuffling and b and b' domains are substrate binding.

17 r moieties, which are essential features for substrate binding.

18 ion, and a C-terminal hydrophobic pocket for substrate binding.

19 against the catalytic domains and preventing substrate binding.

20 of border region between the two domains to substrate binding.

21 ate kinetics analysis, indicating poor lipid substrate binding.

22 ic domain closure of ppGBP was observed upon substrate binding.

23 to play roles in protein stability and SMC3 substrate binding.

24 d active site loops that are responsible for substrate binding.

25 argylic C-H activation occurs directly after substrate binding.

26 phosphate moiety appeared to directly block substrate binding.

27 N219 activity and determines the priority of substrate binding.

28 erved charged residues and are implicated in substrate binding.

29 degree of tunability stem from control over substrate binding.

30 Glu361 and His447 residues are important in substrate binding.

31 s completely closed, seemingly excluding any substrate binding.

32 nd are responsible for initial high-affinity substrate binding.

33 and hCIT subunits function in catalysis and substrate binding.

34 the canonical strand conformation only upon substrate binding.

35 A interdomain linker as indispensable for co-substrate binding.

36 g loop and a P + 1 loop that is important in substrate binding.

37 domains and by electrostatically modulating substrate binding.

38 tural elements contributing to inhibitor and substrate binding.

39 , cell surface GRP78 expression requires its substrate binding activity but is independent of ATP bin

40 ts into a general principle that confers the substrate binding adaptability and specificity to OGA in

41 The gene encoding the E3 ubiquitin ligase substrate-binding adaptor speckle-type POZ protein (SPOP

42 Unexpectedly, substrate binding affinity was dependent upon the Tom40

43 ecific activity is inversely correlated with substrate binding affinity.

44 ATP or ADP binding to the NBD alters the substrate-binding affinity of the SBD, triggering functi

45 on transport, regulation of gene expression, substrate binding and activation, and radical generation

46 "shifted" conformation that is critical for substrate binding and activation.

47 ing assays showed that TH directly binds the substrate binding and carboxyl-terminal domains of Hsc70

48 We identified the essential residues for substrate binding and catalysis and demonstrated that th

49 e recently available, the mechanisms of PgpB substrate binding and catalysis are still unclear.

50 We identify key residues that play roles in substrate binding and catalysis, and rationalize the fun

51 plant homeodomain (PHD) in RAG-2 stimulates substrate binding and catalysis, which are functions of

52 , thus serving as a molecular gatekeeper for substrate binding and catalysis.

53 ength protein was required for correct lipid substrate binding and catalysis.

54 nd confirmed the essential residues used for substrate binding and catalysis.

55 The structural mechanisms determining substrate binding and catalytic activity formed the basi

56 PT1) is available and residues important for substrate binding and catalytic activity have been repor

57 , and Arg(245) to interrogate their roles in substrate binding and catalytic activity.

58 rane-embedded polar residues are crucial for substrate binding and conformational change, the results

59 se loop regions can alter protein stability, substrate binding and even dramatically impact enzyme fu

60 mational preferences which are important for substrate binding and functional activities.

61 o lobes appears not to be a prerequisite for substrate binding and gamma-secretase function.

62 Bepristats reversibly block substrate binding and inhibit platelet aggregation and t

63 ADP/ATP transport, affecting most likely the substrate binding and mechanics of the carrier, respecti

64 tory domains and exposes the active site for substrate binding and other structural changes needed fo

65 the structural basis for phosphatidyl-based substrate binding and phospholipase A activity.

66 r helix moves 'up' and 'down' in response to substrate binding and polymer elongation, respectively,

67 Association of ICP47 precludes substrate binding and prevents nucleotide-binding domain

68 The putative substrate binding and processing site is located on the

69 membrane-mimicking environments interplay in substrate binding and processing.

70 striking conformational changes effected by substrate binding and product release and the role of tw

71 entral shaft, as well as temporal control of substrate binding and product release.

72 ting the existence of a common mechanism for substrate binding and recognition in the P450 superfamil

73 nt of the observed conformational changes in substrate binding and recognition.

74 e tight spatiotemporal coordination of their substrate binding and release cycles.

75 emonstrate that TM6' plays a central role in substrate binding and release on the inner side of the m

76 triggering functionally essential cycles of substrate binding and release.

77 -resolved measurements of intracellular (co-)substrate binding and release.

78 induced allosteric regulation of polypeptide substrate binding and release.

79 binding assay improved our understanding of substrate binding and specificity of the wild-type l-arg

80 ze several of the interconnected properties: substrate binding and specificity, oxidative regioselect

81 s difference and identify residues governing substrate binding and specificity.

82 thyltransferase contains in-line pockets for substrate binding and the active site.

83 The structural basis underlying the natural substrate binding and the most frequent dG:dGTP misincor

84 tructure provides critical insights into the substrate binding and the two-cation-assisted catalytic

85 served transmembrane domains, where multiple substrate binding and translocation features are conserv

86 that HN directly activates CMA by increasing substrate binding and translocation into lysosomes.

87 nsmembrane domain 6 (TM6') a central role in substrate binding and translocation.

88 and mutagenesis studies in combination with substrate binding and transport assays, we identified se

89 Understanding the precise details of substrate binding and turnover in InhA and how this may

90 of the WT flavoprotein, but lack detectable substrate binding and turnover.

91 the kinase-substrate interaction that govern substrate binding and turnover.

92 uggesting that these residues participate in substrate binding and/or catalysis.

93 is of correlated mutations revealed that the substrate-binding and copper-containing surface of LPMO1

94 , it does not inform the structural basis of substrate-binding and kinase activity for diverse substr

95 tion, define rate constants and the order of substrate binding, and demonstrate that the hydroxylatio

96 s such as transcription, translation, ligand-substrate binding, and host immunity.

97 lization of the active site loop involved in substrate binding, and it has been observed that the lev

98 dues around the active site in catalysis and substrate binding, and support a structural model in whi

99 we examine the roles of glycans on activity, substrate binding, and thermal and proteolytic stability

100 The substrate binding appears to be dominated by interaction

101 The effects of substrate binding are observed to be largely sensed at a

102 ates: (i) the "off state," incompatible with substrate binding as seen in the unliganded enzyme; (ii)

103 Substrate binding assays indicated that UDP and fructose

104 recombinant protein unveiled differences in substrate binding between the C. neoformans and human en

105 res revealed similarities and differences in substrate binding between XPA and Rad14.

106 This dimeric ERdj3 shows impaired substrate binding both in the ER and extracellular envir

107 Substrate binding breaks the six-fold symmetry of the co

108 f Galf residues arises not from preferential substrate binding but during processive elongation.

109 of these three pockets occurs after initial substrate binding but precedes catalysis, suggesting a c

110 f interdomain linkers serving a dual role in substrate binding by appropriately positioning the adjac

111 imeric architecture of the complex and donor substrate binding by METTL3.

112 The mechanism by which extracellular substrate binding by SusD proteins is coupled to outer m

113 e have characterized the structural basis of substrate binding by the EcfS subunit for riboflavin fro

114 exibility influences structure, cofactor and substrate binding by the enzyme as well as the structura

115 atalytic N-terminal DHH domain linked to the substrate binding C-terminal DHHA1 domain via an extende

116 d in the absence of CaM, indicating restored substrate-binding capability due to mechanically induced

117 L2' and 130's regions suggested that before substrate binding, caspase-6 exists in a dynamic equilib

118 Thus, substrate binding causes reductive elimination of H2 tha

119 The region spanning Val482 to Glu491 in the substrate-binding cavity and the substrate lid of mortal

120 ity, amino acid changes at the bottom of the substrate-binding cavity have conferred enzyme specifici

121 es cannot rely solely on interactions in the substrate-binding centre to achieve their exquisite spec

122 on, our structure inspires the idea that the substrate-binding channel of AGO3 and consequently its c

123 oA (HiLpoA) has a highly conserved, putative substrate-binding cleft between two alpha/beta lobes.

124 tional yet conserved conformation within the substrate-binding cleft of OGA.

125 barrel catalytic domain that contains a deep substrate-binding cleft tailored to accommodate the hook

126 nt of EcMazF in its inability to support the substrate binding-competent conformation of EcMazF.

127 rous NTPase domains, where it stabilizes the substrate-binding conformation of the P-loop.

128 eric coupling constant (Qax), a ratio of the substrate binding constants in the absence versus presen

129 trolled by the elevator-like movement of the substrate-binding core, along with its wall that simulta

130 PG) ligand that occupies the entire Y-shaped substrate-binding crevice.

131 te in Fe(II)-binding (His5-His62-Glu115) and substrate binding (Cys96-Cys97) are involved in catalysi

132 We find that the observed substrate-binding defect can be rescued by Hsp70/40 chap

133 and simulations to apply tension across the substrate binding domain (SBD) of heat shock protein 70

134 ment point is provided by a KRR-motif in the substrate binding domain.

135 er-domain linker, and the beta-basket of the substrate binding domain.

136 prevent protein aggregation in vitro via its substrate-binding domain (SBD), but the cellular roles o

137 ing domain (NBD), that hydrolyzes ATP, and a substrate-binding domain (SBD), where clients are bound.

138 eotide-binding domain (NBD) and a C-terminal substrate-binding domain (SBD), which are tethered by an

139 g of its nucleotide-binding domain (NBD) and substrate-binding domain (SBD).

140 FBXO31 possesses a unique substrate-binding domain consisting of two beta-barrel m

141 as Esp1) contains four domains (I-IV), and a substrate-binding domain immediately precedes the cataly

142 ng 7 (FBXW7)/hCDC4 mutations within the WD40 substrate-binding domain in 8 of 32 acute ATL patients (

143 Thereby we show that the substrate-binding domain of Mia40 is both necessary and

144 The substrate-binding domain of VioA is mainly responsible f

145 the nucleotide-binding domain and substrate-binding domain) in response to adenine nucleot

146 Adk is rate-limited by slow opening of substrate binding domains and the urea-dependent redistr

147 conformational changes of single and native substrate-binding domains (SBD) of an ATP-Binding Casset

148 e of the ligands dissociates to allow direct substrate binding during turnover is disputed.

149 ary, our findings reveal a substrate-induced substrate-binding event that occurs during the MDDEF-cat

150 essive, which differ in the number of enzyme-substrate binding events needed for complete phosphoryla

151 ted that calcium ions serve to stabilize key substrate-binding extracellular loops.

152 hypothesis that enzymes can use part of the substrate-binding free energy to reduce the entropic pen

153 Substrate binding from the inner leaflet of the bilayer

154 the first time, its nucleotide exchange and substrate-binding functions.

155 elated rotations of processive PMEs, and the substrate-binding groove is negatively not positively ch

156 h block the catalytic center and a conserved substrate-binding groove, respectively.

157 nd LAVP, which block substrate access to the substrate-binding groove.

158 is composed of a GSH binding "G site" and a substrate binding "H site".

159 ys that hybrid CDN-producing and promiscuous substrate-binding (Hypr) GGDEF enzymes are found in othe

160 , the conformational changes that occur upon substrate binding in both enzymes as determined by solut

161 site, respectively, retain sodium-dependent substrate binding in the S1 site similar to the wild-typ

162 An intact bimetallic center and unaltered substrate binding indicate that proper positioning of th

163 However, the mutant undergoes only minor substrate binding-induced heme iron spin state shift tow

164 rpheein model of enzyme hysteresis, in which substrate binding induces conformational changes that pr

165 Here, substrate binding is associated with a significant entro

166 Instead, gamma-secretase-substrate binding is driven by an apparent tight-binding

167 er ion alone hardly contributes to affinity, substrate binding is enhanced for metal-loaded enzymes t

168 adjacent metal hydrides that evolve H2 upon substrate binding is reminiscent of the proposed N2 bind

169 e found that Swc5, although not required for substrate binding, is required for SWR ATPase stimulatio

170 nd whose activity is repressed by a flanking substrate-binding leucine-rich repeat (LRR) domain when

171 adapter domain that stabilizes the aldehyde substrate binding loop and seals the substrate-channelin

172 valent metal ion and Arg124, on the putative substrate binding loop, likely stabilizes the transition

173 it is shown that Ca(2+) ions stabilize a key substrate-binding loop to an even greater degree, as wel

174 enzyme revealed the intact structure of the substrate-binding loop.

175 igher solvent exposure in the regions of the substrate-binding loops L1, L3, and L4 and in the 130s r

176 e revealed by cryo-EM approaches suggested a substrate binding mechanism for NCT, a bilobar structure

177 r example, a putative coenzyme-A-induced-fit substrate binding mechanism mediated by arginine residue

178 site, providing structural insight into PgpB substrate binding mechanism.

179 We also define the ATPase and substrate-binding modalities needed for potentiated Hsp1

180 of varied lipid phosphates exhibited similar substrate binding modes to that of PE, and the residues

181 different human F-box proteins are variable substrate binding modules that determine specificity.

182 In summary, we have identified a unique substrate-binding motif in NleE and OspZ that is require

183 e with GTPase activity and the cofactor- and substrate-binding mutase domains with isomerase activity

184 ctivity, and influence of CypA residues upon substrate binding, mutation, and during catalysis.

185 n CaMKII autophosphorylation, oxidation, and substrate binding nor a change in the phosphorylation of

186 ved in the steady state; neither the rate of substrate binding nor product release was rate-limiting.

187 Substrate binding of the adenine moiety is mediated almo

188 tations that cause an indirect disruption of substrate binding or mildly alter intramolecular interac

189 e main chain hydrogen bonds may help dictate substrate-binding orientation.

190 RNases, Bacillus NrnA has gained an extended substrate-binding patch that we posit is responsible for

191 T1 contributes a distinct substrate-binding pathway with preference for lysine 48-

192 LOX contains a prominent tyrosine in the substrate binding pocket (Tyr(215) in Aerococcus viridan

193 ctions that affect the shape and size of the substrate binding pocket and its mutation has major long

194 s is attributed to the hydrophobicity of the substrate binding pocket and the plasticity of the amino

195 nd phenylalanine residues located within the substrate binding pocket of MdtM may be important for an

196 approach helped identify one residue in the substrate binding pocket of the phosphatase domain that

197 hese results suggest that amino acids in the substrate binding pocket of TrmD underwent an adaptive e

198 region close to the entrance of the proposed substrate binding pocket.

199 of the protein and the size and shape of the substrate binding pocket.

200 ording to current alternating access models (substrate-binding pocket accessible only to one side of

201 Ligation of the substrate-binding pocket by bepristats paradoxically enh

202 ompounds, termed bepristats, that target the substrate-binding pocket of b'.

203 Structure-guided mutagenesis of the proposed substrate-binding pocket of Bmp2 led to a reduction in t

204 ins, revealing conformational changes in the substrate-binding pocket.

205 nases with a unique alpha-helix close to the substrate-binding pocket.

206 ymes with peroxidase-like activity to create substrate binding pockets.

207 ts with the copper site of the LPMO and that substrate binding precludes interaction with CDH.

208 es induced by leukotriene C4, explaining how substrate binding primes the transporter for ATP hydroly

209 is ABC heme importer (HmuUV) and its partner substrate-binding protein (HmuT).

210 se transporters partner with a high-affinity substrate-binding protein (SBP) to import essential micr

211 The substrate binding proteins (SBP) of TRAP transporters ar

212 Periplasmic substrate-binding proteins (SBPs) bind to the specific l

213 ruptor species employ tapirins to complement substrate-binding proteins from the ATP-binding cassette

214 ion of hPHPT1 at Met95, a residue within the substrate binding region.

215 b1, which interacts with GABARAP through its substrate-binding region and promotes K48-linked ubiquit

216 here are large conformational changes in the substrate binding regions of the SET domain, and the K36

217 t observation of the role of amide groups in substrate binding, representing an example of probing ga

218 tion; the compound induces rearrangements of substrate binding residues and of Arg(176), a trigger be

219 qo/Lqo structure to define the catalytic and substrate-binding residues.We also compare the S. aureus

220 s been a shift from active site to secondary substrate binding site (exosite) inhibitor discovery in

221 have properties expected of mutations at the substrate binding site (QL): an increase in the KM of th

222 a,beta-methylene ADP (AMPCP) reveal that the substrate binding site accommodates nucleotides by estab

223 rates that the Ton box and the extracellular substrate binding site are allosterically coupled in Btu

224 pecifically its fluorophenyl ring, in SERT's substrate binding site directly depends on this pocket's

225 Mutations that perturb the substrate binding site either result in the protein bein

226 for abiological catalysis within the natural substrate binding site of an enzyme that can be subjecte

227 structure of murine SMPDL3B, which reveals a substrate binding site strikingly different from its par

228 nd 8 A in translation) lead to access of the substrate binding site to the alternate side of the memb

229 otein because the metal complex occupies the substrate binding site.

230 omain interface previously suggested to be a substrate binding site.

231 moiety allows access to the histone peptide substrate binding site; incorporation of a conformationa

232 substrate interaction, mutating the putative substrate-binding site in a constitutively active Hsp104

233 ed at different positions within the central substrate-binding site of hSERT, while no crosslinking i

234 itors 10d and (S)-17b, which bind within the substrate-binding site of MMP-13 and surround the cataly

235 ogues revealed selective binding to the NNMT substrate-binding site residues and essential chemical f

236 the extracellular space and reconfigures the substrate-binding site such that it relinquishes its aff

237 alF-MalG and the alternate exposition of the substrate-binding site to either side of the membrane.

238 shion in the absence of ligand to expose the substrate-binding site to the extracellular milieu.

239 ing movement of two bundles around a central substrate-binding site, it has become clear that even th

240 substrates demonstrate the plasticity of the substrate-binding site, which confers substrate specific

241 sly shown to act at a site separate from the substrate-binding site.

242 de cross-linked by Hexim1 corresponds to the substrate binding-site.

243 ent detailed information on the cofactor and substrate binding sites and on the catalytic mechanisms

244 with two of them, Trp-40 and Trp-38, in the substrate binding sites near the tunnel entrance.

245 or ACE had corresponded to the catalytic and substrate binding sites of the two enzymes.

246 lude descriptions of cofactor binding sites, substrate binding sites, and catalytic mechanisms.

247 onformations with inward- and outward-facing substrate-binding sites in response to engagement and hy

248 lenges exist: immobilizing enzymes can block substrate-binding sites or prohibit conformational chang

249 ed by the diversity and flexibility of their substrate-binding sites, motivating research into their

250 tification of crucial water molecules in the substrate-binding sites, unveiling their functional role

251 features an allosteric network that couples substrate-binding sites.

252 presents exofacial (e2) and endofacial (e1) substrate-binding sites.

253 166 (p.Q166E) has been reported to alter the substrate-binding specificity by shifting Glut5-mediated

254 We show, however, that substrate binding stabilizes the resultant Fe-H2O2 compl

255 2 region converting caspase-6 to a competent substrate-binding state.

256 The substrate binding stoichiometry of the bacterial NSS pro

257 ippel-Lindau tumor suppressor protein is the substrate binding subunit of the VHL E3 ubiquitin ligase

258 posed of four subunits: an integral membrane substrate-binding subunit (EcfS), a transmembrane coupli

259 ing a G2/M cyclin, cyclin F functions as the substrate-binding subunit of SCF(cyclin F) E3 ubiquitin

260 of SecB chaperone function suggests that its substrate binding surface can be readily remodeled to ac

261 f H2O2 Our results show that residues on the substrate-binding surface of LPMOs have co-evolved to op

262 phate, we surprisingly identify a bifurcated substrate-binding surface that explains structured subst

263 n revealed two distinct orientations for the substrate binding that define reverse or normal prenylat

264 This effect could be explained by productive substrate binding that protects LPMOs from oxidative sel

265 in the strand conformation before and after substrate binding, the hydrogen/deuterium exchange data

266 ration of Parma hams seems to depend on such substrate binding to BsFECH and was facilitated by limit

267 t molecular mechanisms coupling cosubstrate/ substrate binding to catalytic activity.

268 PYNT sequence is proposed to interfere with substrate binding to Cdk9 and thereby to inhibit its kin

269 Here we present the detection of substrate binding to cytochrome P450-2J2 (CYP2J2), the p

270 complement our transport assays by measuring substrate binding to detergent-purified SbMATE protein.

271 Substrate binding to FliT activates the complex for FlhA

272 equently, p53 competes with IkappaBalpha for substrate binding to IKKbeta and thereby blocks IkappaBa

273 ctures coinciding with initial peptidoglycan substrate binding to PBP2a, acyl enzyme formation, and a

274 complete cellulose biosynthesis cycle, from substrate binding to polymer translocation.

275 TonB-dependent transporter for vitamin B12, substrate binding to the extracellular surface unfolds a

276 ith the wild-type enzyme, demonstrating that substrate binding to the high-energy state is not occlud

277 bolished by the Y662A mutation that disrupts substrate binding to the NBD2 pore loop.

278 nhibited by the Y257A mutation that disrupts substrate binding to the nucleotide-binding domain 1 (NB

279 otide-induced asymmetry is a requirement for substrate binding to the pore loops and that recruitment

280 i PglB offer new experimental information on substrate binding to the related, but structurally uncha

281 ed electron transfer reactions occur without substrate binding to the ruthenium center, but instead w

282 their intracellular juxtamembrane region and substrate binding to this region.

283 Absorption spectroscopy of seven different substrates binding to CYP2J2 in solution showed that the

284 P1-CUL1-F-box)/CRL1 substrates that promotes substrates binding to F-box, epidermal growth factor (EG

285 effects mediated by direct participation in substrate binding, to more distributed effects that prop

286 Our data also implied that the substrate-binding transmembrane helices move up to 10 A

287 rocess is the elevator mechanism, in which a substrate-binding transport domain moves a large distanc

288 veil a dynamic Tdp2 active site lid and deep substrate binding trench well-suited for engaging the di

289 embly of the active Tat translocase in which substrate binding triggers replacement of TatB by TatA a

290 in the 2nd SIA-binding site, indicating that substrate binding via this site enhances NA catalytic ac

291 ons in the heme-binding (R374W and R448C) or substrate-binding (W116C) site of 11beta-hydroxylase, or

292 Selective substrate binding was further confirmed by isothermal ti

293 not transport lysophosphatidic acid, and its substrate binding was not inhibited by either orthophosp

294 The participation of domain C(+) in substrate binding was supported by reduced substrate inh

295 with its impact on the Kd for linoleic acid substrate binding, we conclude that OS binding brings ab

296 atalysis compensate for entropic losses from substrate binding while facilitating sampling of the tra

297 le hexamers within the complex, functions in substrate binding with strong affinity for PLs, and modu

298 YP144A1-TRV has an open structure primed for substrate binding, with a large active site cavity.

299 tallographic, dynamic and kinetic aspects of substrate binding within porous MOFs.

300 The substrate binding yields characteristic Cotton effects t