ライフサイエンスコーパス: substrate preference

1 of ATP production, it can also modify energy substrate preference.

2 group of deubiquitin proteases with distinct substrate preference.

3 te lyase, suggests potential determinants of substrate preference.

4 cap domain contributes residues that enforce substrate preference.

5 differed widely with regard to activity and substrate preference.

6 dium thermocellum (CthTTM) with the opposite substrate preference.

7 cteria were characterized to determine their substrate preference.

8 chimera was functional and displayed a novel substrate preference.

9 the functional significance of this shift in substrate preference.

10 showed that each contributed to the altered substrate preference.

11 the C-terminal lipase domain in lipoprotein substrate preference.

12 it is not sufficient to encode the change in substrate preference.

13 enesis did not affect either localization or substrate preference.

14 subfamilies with divergence in sequence and substrate preference.

15 xamine in detail the role of TM11 in setting substrate preference.

16 wo enzymes based primarily on differences in substrate preference.

17 th on a molecular basis and by pharmacologic substrate preference.

18 CelY and CelZ were quite different in substrate preference.

19 regulation of its enzymatic activity and/or substrate preference.

20 e for both GTP and CTP, reflecting a loss of substrate preference.

21 le E loop, Asp-147 and Glu-149, modulate the substrate preference.

22 d not alter its intrinsic 5'-CC dinucleotide substrate preference.

23 te structures that underlie the variation in substrate preference.

24 tive site entrance also apparently influence substrate preference.

25 underlie subtle or drastic changes in pgFAR substrate preference.

26 riple mutants, suggesting their diversity in substrate preference.

27 e importance of this hydrophobic feature for substrate preference.

28 rters surprisingly exhibit differential tRNA substrate preferences.

29 and CELA3B isoforms did not evolve distinct substrate preferences.

30 mes and provide valuable insights into their substrate preferences.

31 mong these enzymes based on their respective substrate preferences.

32 ies differ in their oligosaccharide acceptor-substrate preferences.

33 enzymes with regard to cleavage patterns and substrate preferences.

34 ese enzymes have both overlapping and unique substrate preferences.

35 CYP1As are orthologous enzymes with similar substrate preferences.

36 li peptidoglycan amidases may have different substrate preferences.

37 ew instances of COMT-like enzymes with novel substrate preferences.

38 MMPs previously screened, MMP-20 had unique substrate preferences.

39 ions resulted in distinct alterations in PC2 substrate preferences.

40 temperature profiles and differing relative substrate preferences.

41 ansferase is surprisingly promiscuous in its substrate preferences.

42 hich is critical to understanding their true substrate preferences.

43 istent with these biochemical activities and substrate preferences.

44 e a structural understanding of the observed substrate preferences.

45 xpression, and each enzyme exhibits distinct substrate preferences.

46 oxylation activity into P450s with different substrate preferences.

47 residue in the two enzymes can change their substrate preferences.

48 region of GCC and MCC explain their distinct substrate preferences.

49 protein homo- and heterodimers with distinct substrate preferences.

50 understanding of its catalytic mechanisms or substrate preferences.

51 ts of AUM and chronophin that explains their substrate preferences.

52 hanism; however, the enzymes differ in their substrate preferences.

53 nsible for alterations in neuronal metabolic substrate preferences.

54 To elucidate substrate preferences, a panel of deletion mutations and

55 Although ADAMs showed substrate preference (ADAM17, TGFalpha and heparin-bindi

56 mmalian orthologues revealed conservation of substrate preferences against a panel of peptide and gly

57 rovide knowledge about critical residues and substrate preference among CCRs and provide, to our know

58 ations help define the structural origins of substrate preference among Eis homologues and suggest th

59 sLac's substrate preference among monoaryls was also consistent

60 We further propose that the distinct substrate preferences among Cas2 proteins may be determi

61 mendments at both depths, but with different substrate preferences among lineages.

62 h identified critical residues and explained substrate preferences among PAL isozymes in sorghum and

63 rate recognition motifs determines enzymatic substrate preference and catalysis.

64 They varied in substrate preference and catalytic activity.

65 egionella, Pseudomonas, and fungi with broad substrate preference and function in virulence.

66 and oxaloacetate, Ab-ArAT4 possesses strong substrate preference and highest activity with the aroma

67 d to possess an enzyme activity with similar substrate preference and insensitivity to cyclosporin A.

68 tion and divergence, gene loss, evolution of substrate preference and promiscuity.

69 and near-perfect correlation between the MMP substrate preference and sequence identity of 50-57 disc

70 A comparison of whole-body substrate preference and skeletal muscle substrate oxida

71 with different EED isoforms exhibit similar substrate preference and specificity.

72 , and Cw24) were compared for their relative substrate preferences and cleavage kinetics using eIF4G

73 , each having however clear specificities of substrate preferences and kinetic properties.

74 Recombinant proteins displayed distinct substrate preferences and product specificities that can

75 The results allow insight into substrate preferences and regulation of DAPK, provide a

76 igh level expression, biochemical mechanism, substrate preference, and regulation.

77 t into the enzymatic properties of MarP, its substrate preference, and the importance of its transmem

78 m1p possess similar domains and show similar substrate preferences, and both localize to the nuclear

79 owth rates, gene expression profiles, carbon substrate preferences, and cell-cell signaling profiles.

80 structural domains to RNA product length and substrate preference are incompletely understood, due in

81 ulated transporter networks with overlapping substrate preferences are involved in sensing and signal

82 share 30% sequence identity; however, their substrate preferences are varied.

83 OsHPLs also differ in their substrate preference as determined by in vitro enzyme as

84 membrane domains of GlcNAc6ST-2 had the same substrate preference as native GlcNAc6ST-1.

85 proline + 4" residue within TM4 to determine substrate preference at the exit gate.

86 idue (Gly-553) in this pocket can change the substrate preference between short- and medium-chain acy

87 Regulatory Factor 1 (Smurf1) can switch its substrate preference between two proteins of opposing ac

88 We confirmed the reversed substrate preference by determining the Michaelis-Menten

89 Furthermore, our results suggest substrate preference by NgTET1 to (5m)CpG and TpG dinucl

90 4,5,6-tetrakisphosphate enantiomers and that substrate preference can be manipulated by Arg(130) muta

91 gatus CYP51B, including determination of its substrate preferences, catalytic parameters, inhibition,

92 During heart failure (HF), cardiac metabolic substrate preference changes from fatty acid (FA) toward

93 sident MyrAkt1 exhibited a markedly distinct substrate preference compared with MyrAkt1 immunoprecipi

94 tained from such in silico models, including substrate preference, consequences of gene deletions, op

95 horylation of the E3 ligase could switch its substrate preference, contributing to selective protein

96 ost of the analyzed enzymes displayed narrow substrate preferences corresponding to their predicted p

97 than ceramide based on in vitro assays with substrate preference deoxycholic acid > chenodeoxycholic

98 , A, B, and C, distinguished by their unique substrate preferences despite the fact that the structur

99 sed in E. coli, purified, and their in vitro substrate preferences determined.

100 -dependent endonuclease activity and display substrate preferences different from MspJI.

101 The substrate preferences displayed by these enzymes toward

102 aterial revealed that tryptase epsilon has a substrate preference distinct from that of its other fam

103 latforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading

104 thought that the pathogenic mutations alter substrate preference (e.g. ATP versus ADP) thereby domin

105 We found that tissue-specific substrate preference exists in PS synthesis.

106 ically recognizes CpG dinucleotide and shows substrate preference for 5mC in a CpG context.

107 the recombinant PP2Cs exhibited a comparable substrate preference for a phosphothreonine containing s

108 raight-chain fatty acids (SCFAs), FabH has a substrate preference for acetyl-CoA.

109 -glucosidase, a member of GH31 family, shows substrate preference for alpha(1-6) over alpha(1-4) glyc

110 The first, which demonstrated substrate preference for Ang I, was neutral endopeptidas

111 Ang-(1-7)-forming enzymes that demonstrate substrate preference for Ang II are likely to play an im

112 function is often associated with a shift in substrate preference for ATP production.

113 Mitochondrial proteases demonstrated a substrate preference for basic protein variants, which i

114 EET substrate preference for both COX-1 and COX-2 were estim

115 that this P450 is a omega-hydroxylase with a substrate preference for both saturated and unsaturated

116 acyl-specific phospholipid transacylase with substrate preference for cardiolipin and phosphatidylcho

117 MMP-13 is a collagenase with a substrate preference for collagen II over collagens I an

118 The enzyme shows no substrate preference for dehydroepiandrosterone versus p

119 However, UvrD had a substrate preference for fork structures having a nascen

120 ther, our results indicate that the acquired substrate preference for GAB1 is critical for the ERBB2

121 sion of a mutant form of Hs2st with a strong substrate preference for GlcA-containing units, suggesti

122 logs with respect to gelatinolytic activity, substrate preference for hydrophobic amino acids on both

123 or PGPH) activity and identified an overall substrate preference for hydrophobic residues at the P1

124 2-O-sulfotransferase (Hs2st) shows a strong substrate preference for IdoA over GlcA, C5-epimerizatio

125 A change in substrate preference for K147Q SS pyruvate lyase activit

126 a3 (At1g51440), a plastid lipase with a high substrate preference for MGDG, and is sustained by furth

127 ducts, providing a rationale for its unusual substrate preference for NaMN over NaAD.

128 ed that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphosphates UTP, G

129 encodes an acyl-ACP synthetase (AasC) with a substrate preference for palmitic compared with oleic ac

130 ipids into murine fibroblasts, with a strong substrate preference for phosphatidylserine.

131 ayed diphosphate phosphatase activity with a substrate preference for PSDP > FDP > phosphatidic acid.

132 phosphatase/phosphotransferase with distinct substrate preference for PSDP.

133 Collectively, the substrate preference for subsites (P(3)-P(4)') of C. his

134 ults obtained with PCAF demonstrate a strong substrate preference for the N-terminal residues of hist

135 Delta(10)(trans) double bond and displays a substrate preference for the trans-Delta(12), rather tha

136 range of cellular proteins, has a remarkable substrate preference for translation-related proteins (e

137 It displays a substrate preference for two molecules of indole-3-pyruv

138 o define more specific structural details of substrate preferences for binding and catalysis, we have

139 n shown that Dnmt3a and Dnmt3b have distinct substrate preferences for certain genomic loci, includin

140 s suggest that the frequent inversion of the substrate preferences for nonadiabatic photoheterolysis

141 e enzyme-peptide complex explains the marked substrate preferences for particular P4, P2 and P1 resid

142 Here we investigate the substrate preferences for Pif1p.

143 mbers of this enzyme family show distinctive substrate preferences for short-, medium- or long-chain

144 s to explore the chemical space that defines substrate preferences for the auxin uptake carrier AUX1.

145 s an in vitro enzyme assay detected possible substrate preferences for the endopeptidase penicillin b

146 Surprisingly, the substrate preferences for the human and mouse enzyme are

147 glycoside kinases, rationalize the different substrate preferences for these enzymes.

148 The three enzymes have very similar substrate preferences for three optimized peptide substr

149 tures WRN acts upon in vivo, we examined its substrate preferences for unwinding.

150 the perinatal period redirect mitochondrial substrate preference from carbohydrates to fatty acids.

151 hough in acute ischemia there is a switch in substrate preference from fatty acids to glucose, metabo

152 Shift of myocardial substrate preference has been observed in many chronic d

153 In support of its DNA substrate preference, helicase sequestration studies rev

154 The CYP51 substrate preferences imply differences in the post-squa

155 among transferases, thus further modulating substrate preference in an isoform-specific manner.

156 ns, suggesting that the Zn(2) site modulates substrate preference in mbetal L1.

157 A method has been developed to determine the substrate preference in phosphatidylserine decarboxylati

158 In this study, substrate preference in PS synthesis was determined to g

159 blish for the first time that there exists a substrate preference in PSD in liver (18:0,18:1 > or = 1

160 les in insulin-stimulated glucose uptake and substrate preference in skeletal muscle and adipose cell

161 t modulates fatty acid metabolism, regulates substrate preference in the heart.

162 19W/G301F-SbCAD4 double mutant displayed its substrate preference in the order coniferaldehyde > p-co

163 ollowed by 18:0,22:5-PC, resulting in the PC substrate preference in the order of 18:0,22:6 > 18:0,22

164 AWAT1 and AWAT2 have very distinct substrate preferences in terms of alcohol chain length a

165 combinant mTORC1 and mTORC2 exhibit distinct substrate preferences in vitro, consistent with their ro

166 itro, the enzymes have distinct glycoprotein substrate preferences in vivo.

167 w strong hydrolytic activity with a broad P1 substrate preference including basic and hydrophobic gro

168 also repair other lesions and have distinct substrate preferences, indicating that they have potenti

169 ntions have been tested clinically to target substrate preference, insulin sensitivity, and mitochond

170 and full-length proteins suggest that HDAC8 substrate preference is based on a combination of short-

171 In B-cell lymphomas, its substrate preference is frequently altered through somat

172 er, there has been debate as to whether this substrate preference is indicative of unidirectional tra

173 exact nature of the mutations, the enzyme's substrate preference is modified.

174 yzed simultaneously, (ii) prior knowledge of substrate preference is not required, (iii) information

175 Interestingly, this substrate preference is preserved when using a released

176 rized to date, the molecular basis for their substrate preferences is unknown.

177 ynC, which was characterized with respect to substrate preference, kinetic properties, and product fo

178 mportance of the stem region with respect to substrate preference, localization, and oligomerization.

179 Despite their different substrate preferences, many NRTKs are structurally simil

180 volutionarily related proteases with similar substrate preferences may have distinct biological roles

181 onal groups (BFGs) each distinguished by its substrate preferences, metabolic pathways and its prefer

182 To compare kinase substrate preferences more generally, we employed a prot

183 e contains a sequence derived from known Syk substrate preference motifs linked to a cell permeable p

184 ss-talk, by which effector binding regulates substrate preference, occurs largely through R293 and Q2

185 sphopeptide chips, to determine the in vitro substrate preference of 16 members of the protein-tyrosi

186 million nucleotides that contributes to the substrate preference of a coenzyme A ligase.

187 tyrosine as free amino acid and altering the substrate preference of a prenyltransferase by mutagenes

188 Biochemical analysis demonstrates that the substrate preference of AtGH3.5 is wider than originally

189 ied Ydr109c and FGGY proteins showed a clear substrate preference of both kinases for d-ribulose over

190 te residues that appear to contribute to the substrate preference of CCMTs relative to other members

191 3 and 4 (TM3-4) of Drs2 into Dnf1 alters the substrate preference of Dnf1 from PC to PS.

192 d to the trans-Golgi network and adopted the substrate preference of GlcNAc6ST-1.

193 ion (L487A/P488A) is required to convert the substrate preference of hGS from beta-Ala to Gly.

194 reveal the molecular changes that define the substrate preference of hGS, explain the product diversi

195 lphaPhe55 to alanine dramatically alters the substrate preference of MADH.

196 s provided a mechanistic explanation for the substrate preference of MKP-2 and suggest that catalytic

197 s sufficient to enact a global change in the substrate preference of one MMP to that of another, indi

198 adenylation assay, we characterized the acyl substrate preference of PBS3.

199 tion of kinetoplastid cells suggest that the substrate preference of TBCYP51 may reflect a novel ster

200 Biochemical analyses indicate that the substrate preference of TET2 results from the different

201 Our studies demonstrate that the substrate preference of TET2 results from the intrinsic

202 The specific activity and substrate preference of the bacterially expressed enzyme

203 m region of GlcNAc6ST-1 affects the cellular substrate preference of the enzyme without altering its

204 on has minor effects on the structure or the substrate preference of the enzyme.

205 ure are important factors in determining the substrate preference of the EZH2 histone methyltransfera

206 ed, such as the newly uncovered bifunctional substrate preference of the key regulatory enzyme in toc

207 mino acids by mutagenesis, characterized the substrate preference of the mutants, and determined the

208 Intrigued by the novel substrate preference of the Sicarius enzyme, we solved i

209 to the viral activators does not change the substrate preference of these kinases.

210 To better understand the substrate preference of these toxins, we used (31)P NMR

211 The intriguing substrate preference of this enzyme for nicked Holliday

212 In this study, the substrate preference of this enzyme was investigated by

213 Substrate preferences of 14 kinases mainly from the FGGY

214 cleotides can be used to rapidly examine the substrate preferences of a given glycosylase.

215 -linking approach to probe the structure and substrate preferences of AlkB and its three human homolo

216 We investigated the substrate preferences of bacterial aryloxyalkanoate diox

217 llows us to quantitatively determine the DNA substrate preferences of cytosine methylases.

218 eIF4G, acts as a modulator for activity and substrate preferences of Ded1p, which is the RNA remodel

219 We show that the exosome exploits distinct substrate preferences of DIS3 and RRP6, its two catalyti

220 The quaternary structure and substrate preferences of MERS-CoV PLpro were determined

221 sense mutation in an ancestral ape, compared substrate preferences of mouse and human marapsins with

222 Recently, crystal structures and substrate preferences of NS2B-NS3pro from Dengue and Wes

223 af and seed tissues, protein properties, and substrate preferences of plant cyclopropane synthase wer

224 nosoma cruzi, and L. infantum) suggests that substrate preferences of plant- and fungal-like protozoa

225 side of the site of hydrolysis, we profiled substrate preferences of recombinant human chymase using

226 t biological soil crusts (biocrusts) and the substrate preferences of seven biocrust isolates.

227 s from Trypanosomatidae, dramatically alters substrate preferences of TCCYP51, converting it into a m

228 acid compositions of the mutants reveal the substrate preferences of the desaturase and elongase enz

229 al analyses provide unique insights into the substrate preferences of the distinct active sites and h

230 he reasons for the CELA3 duplication and the substrate preferences of the duplicated isoforms are unc

231 Here we report a characterization of the substrate preferences of the enzyme complex using a reco

232 Here we biochemically characterize the substrate preferences of the helix-hairpin-helix (HhH) d

233 ne ribosides and cytokinins that reflect the substrate preferences of the knocked out enzymes.

234 tri-methylation (H3K27me3) owing to altered substrate preferences of the mutant enzymes.

235 ed under various conditions to determine the substrate preferences of the OppA proteins.

236 peptide-aminomethylcoumarins to contrast the substrate preferences of the recombinant Mtb proteasome

237 the cap domain, implying differences in the substrate preferences of the two enzymes.

238 convert Ado to Ade, an understanding of the substrate preferences of these enzymes could lead to the

239 We investigated the substrate preferences of these PPIases in vitro using ty

240 A wrap, and also suggest that the particular substrate preferences of topoisomerase IV might be dicta

241 n RecQ family helicases, we have studied the substrate preferences of two closely related members of

242 cognition of a primary cognate sequence, the substrate preferences of two DUBs, UCH-L3 and isopeptida

243 d GC adhesion, initial axonal outgrowth, and substrate preference on alternating matrix stripes and m

244 is not required, (iii) information regarding substrate preferences on both side of the scissile bond

245 pical for AANAT family members, although the substrate preference pattern was somewhat broader, the s

246 F) / feeder-free conditions and evaluated XF substrate preference, pluripotency, and karyotype.

247 e larval swimming, or to the CNS to regulate substrate preference prior to the induction of larval se

248 l known TUTases, nucleotide specificity, RNA substrate preference, processivity, quaternary structure

249 The substrate preference profile of the St-IVD2 protein was

250 ors into account, our data reveal that pgFAR substrate preference provides a good explanation of how

251 To a certain extent, their substrate preference redundancies correlate with structu

252 ve to Holliday junction substrates, and that substrate preference reflects binding affinity and maps

253 F) were produced that exhibited the opposite substrate preference relative to the respective native e

254 that originally displayed the much narrower substrate preferences required for glycogen catabolism.

255 lasmin and thrombin was used to validate the substrate preferences resulting from the PS-SCL.

256 stinguished, because they differ in acyl-CoA substrate preference, sensitivity to inhibition by dihyd

257 differences that give rise to the individual substrate preferences shown by these highly related isoe

258 otransferase exhibited specific activity and substrate preferences similar to the wild type bovine Gl

259 ects enzyme structure and dynamics, and thus substrate preference, simultaneously and sequentially.

260 Substrate preference studies show that nocturnin is an e

261 ution patterns, intracellular locations, and substrate preferences, suggesting that each isoform has

262 lar regulatory events involved in the energy substrate preference switch from fatty acids to glucose

263 This substrate preference switch is mediated by the membrane

264 The two enzymes have differences in their substrate preferences that explain the variations observ

265 rapid in vitro assay, thereby demonstrating substrate preferences that overlapped but were clearly d

266 The two enzymes have differences in their substrates preferences that explain variations observed

267 Despite this difference in substrate preference, the degree of relaxation of the hy

268 nervous systems may alter the cardiac energy substrate preference, thereby contributing to the progre

269 The co-purified cRDH showed marked substrate preference to 11-cis-retinal and preferred NAD

270 There is a switch of gluconeogenic substrate preference to glycerol that quantitatively acc

271 calling for other mechanisms that coordinate substrate preference to maintain a functional TCA cycle.

272 tio of OASS:CAS activity but did not convert substrate preference to that of a CAS.

273 ' the base of each effector and communicates substrate preference to the active site by forming diffe

274 variants displayed 18- to 19-fold shifts in substrate preference toward 5FC, a significant reduction

275 Importantly, vertebrate EBAX also shows substrate preference toward aberrant Robo3 implicated in

276 A shift of substrate preference toward glucose in the heart is cons

277 of the metabolic network to favor a shift of substrate preference toward glucose.

278 Cryptosporidium ACSs displayed substrate preference toward long-chain fatty acids.

279 albicans, and MT isoforms, reveals profound substrate preference toward obtusifoliol (turnover 5.6 m

280 showed that recombinant BAR and PAT exhibit substrate preference toward phosphinothricin over the 20

281 d by thin layer chromatography analysis with substrate preference toward unsaturated fatty acids.

282 The enzyme exhibits a strong substrate preference toward xylooligosaccharides; hence

283 s, however, PfSET7 displays specific protein substrate preference towards nucleosomes with pre-existi

284 nal (C terminal) domain did not change lipid substrate preference (triglyceride vs. phospholipase) as

285 gest a role for adropin in regulating muscle substrate preference under various nutritional states.

286 ctivity, we analyzed Prp enzyme kinetics and substrate preference using a fluorogenic peptide cleavag

287 groups to the sterol C-24 position, and the substrate preference was found to be a unique property o

288 Although the observed substrate preference was to Delta(2)-trans,Delta(4)-tran

289 This degree of substrate preference was unique to DEF-1, as other ARF G

290 Based on the substrate preference, we have named it NEH1 (Nei homolog

291 To better understand these substrate preferences, we present crystal structures of

292 Residues that determine IAA versus BA substrate preference were identified.

293 The library-derived substrate preferences were applied in a genome-wide sear

294 erization of four new enzymes revealed their substrate preference, whereas their catalytic residues w

295 that even closely related enzymes have clear substrate preferences with AKR7A2, AKR7A4, and AKR7A5 sh

296 igm for natural molecular rulers and imparts substrate preferences with ramifications for biological

297 ative and recombinant PjapPDE showed a clear substrate preference, with an estimated half-life in viv

298 Abs indicated divergent activity levels and substrate preferences, with the common requirement of a

299 and activation energies indicated different substrate preferences within secreted MMPs, because MMP-

300 x, providing a mechanism to evolve different substrate preferences within the family without large st