ライフサイエンスコーパス: substrate specificity

1 substitutions in human RR additively affect substrate specificity.

2 ich use quality control pathways to maintain substrate specificity.

3 ng that the C-terminal domain has a distinct substrate specificity.

4 oligomerization might lead to differences in substrate specificity.

5 he LLO and region 1 to influence polypeptide substrate specificity.

6 nthetase-tRNA pairs with mutually orthogonal substrate specificity.

7 ide hydrolase family 31 (GH31) and differ in substrate specificity.

8 remarkably similar hydrolase activities and substrate specificity.

9 zation of sequence-level determinants of MMP-substrate specificity.

10 of the protein, thereby providing exquisite substrate specificity.

11 h high catalytic efficiency and well defined substrate specificity.

12 he structural determinants of polysaccharide substrate specificity.

13 plays a significant role in determining the substrate specificity.

14 a single amino acid substitution that alters substrate specificity.

15 nowledge, into the determinants for acyl-CoA substrate specificity.

16 e glutamines and probe for sources of FXIIIa substrate specificity.

17 y of the transporters accompanied changes in substrate specificity.

18 structure is a major determinant of sirtuin substrate specificity.

19 t and illustrated the molecular basis of the substrate specificity.

20 ntial elements underlying its unusually high substrate specificity.

21 ynthesis of estrogen from androgen with high substrate specificity.

22 than nucleobase sequence, usually determines substrate specificity.

23 However, MTases typically possess tight substrate specificity.

24 cupied by dTMP and dCMP resolving aspects of substrate specificity.

25 r DNA of chromatin, thereby controlling Set2 substrate specificity.

26 e phosphagen specificity loop is crucial for substrate specificity.

27 e N-glycan intermediates with relative broad substrate specificity.

28 d conformational changes, ion selectivity or substrate specificity.

29 affinity RPA DBDs at forks dictates SMARCAL1 substrate specificity.

30 al transduction is regulated at the level of substrate specificity.

31 f glutamate carboxypeptidase II yet distinct substrate specificity.

32 d amino acids are mainly responsible for its substrate specificity.

33 nzymes, lasso peptide isopeptidase has broad substrate specificity.

34 amino acid residues of GLUT5 that define its substrate specificity.

35 g its active conformation, thereby effecting substrate specificity.

36 ic active site, protein oligomerization, and substrate specificity.

37 six ceramide synthases (CerS) that differ in substrate specificity.

38 d throughout an active site to alter enzymic substrate specificity.

39 fork and flap structure cleavage by relaxing substrate specificity.

40 y is essential to the acquisition of broader substrate specificity.

41 e amino acid significantly changed the PPO's substrate specificity.

42 constrained our ability to robustly engineer substrate specificity.

43 alloproteases (ADAMs) is regulated with high substrate-specificity.

44 of synthetic and lytic enzymes with varying substrate specificities.

45 he proteasomes have overlapping but distinct substrate specificities.

46 hich the majority have unknown functions and substrate specificities.

47 s can demonstrate a great deal of overlap in substrate specificities.

48 sms governing their enzymatic activities and substrate specificities.

49 a succession of CDK complexes with different substrate specificities.

50 tructurally similar, yet they have different substrate specificities.

51 at lead to unique active site topologies and substrate specificities.

52 ne organization, yet with a wide spectrum of substrate specificities.

53 acyl-CoAs with both distinct and overlapping substrate specificities.

54 e number of isozymes with ranges of in vitro substrate specificities.

55 uggesting that these effectors have distinct substrate specificities.

56 SbCYP82D2 is an F8H with high substrate specificity, accepting only chrysin as its sub

57 provides a structural basis for the observed substrate specificity, accommodating a 10-carbon fatty a

58 minal insights into its catalytic mechanism, substrate specificity, allosteric regulation, and inhibi

59 r THP provides a framework for understanding substrate specificity among numerous NMTs involved in th

60 on at residue 357 (S357Y), PRDM7 regains the substrate specificities and catalytic activities similar

61 binding domains, the dsRBPs exhibit diverse substrate specificities and dynamic properties when in c

62 ubiquitin/ubiquitin-like complexes, explain substrate specificities and redefine relationships acros

63 tive cytidine deaminases that exhibit unique substrate specificities and thermosensitivities.

64 s the use of endoglycoceramidases with broad substrate specificity and a robust workflow that enables

65 The mechanisms underlying substrate specificity and activity in the tolloid family

66 lloid-like (TLL)-1 and TLL-2 differ in their substrate specificity and activity levels, despite shari

67 in monolignol biosynthesis, the variation in substrate specificity and activity of CAD can result in

68 By engineering the substrate specificity and activity of the pathway enzyme

69 s the potential signature sequence for their substrate specificity and activity.

70 Recombinant proteins were shown to have high substrate specificity and affinity for picrinine, conver

71 spite intense investigation, many aspects of substrate specificity and catalysis remain unclear.

72 We establish the orthogonality, substrate specificity and catalytic competency of the en

73 dentify amino acids that influence CoA ester substrate specificity and contribute to differences in t

74 iding the yeast protein-recovery system with substrate specificity and efficiency in ATP consumption.

75 AT-less type I PKS KSs possess substrate specificity and fall into phylogenetic clades

76 the T154Y mutation in SbCCR1 led to broader substrate specificity and faster turnover.

77 propose that other domains of RHOGAPs confer substrate specificity and fine-tune their catalytic effi

78 r understanding the structural basis of LPAT substrate specificity and for generating altered oil fun

79 Enzyme shows a broad substrate specificity and hydrolyzes both natural and sy

80 rthologs, Corallochytrium Csk displays broad substrate specificity and inhibits Src in an activity-in

81 ikDH2 was shown to possess remarkably strict substrate specificity and is unable to turn over substra

82 ganization of APOBEC3B catalytic domain, its substrate specificity and its possible role in causing g

83 reviously shown that the N-Srcs have altered substrate specificity and kinase activity compared to C-

84 The kinetic data unravel the so far unknown substrate specificity and mechanism of halide oxidation

85 Its substrate specificity and mechanism were interrogated wi

86 We describe here the factors that govern the substrate specificity and order of biosynthetic events t

87 e catalytic cleft play a significant role in substrate specificity and ordered Gag processing.

88 ify critical determinants of acyl-CoA mutase substrate specificity and predict new acyl-CoA mutase-ca

89 ylobiose), the residues that tune the unique substrate specificity and regioselectivity could be iden

90 To date, HDAC substrate specificity and selectivity have been largely

91 ural underpinning of DH domains that control substrate specificity and selectivity remains limited, t

92 dy provides the structural basis for SMPDL3A substrate specificity and sheds new light on the functio

93 Therefore, better definition of the substrate specificity and structural properties of the P

94 er diacylglycerol control by the distinctive substrate specificity and subcellular distribution of PK

95 hese results expand our understanding of the substrate specificity and the catalytic scope of acyl-Co

96 nal ancillary domain, the latter determining substrate specificity and the localization of the protea

97 However, their substrate specificity and the mechanism involved in GQ u

98 anism, including the factors determining the substrate specificity and the pH-dependence of the catal

99 PKD2 increases turnover for Syntide-2, while substrate specificity and the role of PKD2 in NF-kappaB

100 D184Q, were found to have remarkably relaxed substrate specificity and were capable of transferring t

101 Its substrate-specificity and tissue-specific expression mak

102 by the BBB include molecule size, polarity, substrate specificity, and active efflux mechanisms.

103 vely) to promote complex formation, restrict substrate specificity, and enhance enzymatic activity.

104 aled no differences in catalytic efficiency, substrate specificity, and inhibition.

105 bunits that determine subcellular anchoring, substrate specificity, and physiological function.

106 tes contributes to the enzyme's activity and substrate specificity, and reveal that the presence of d

107 domain impact the proteolytic activity, the substrate specificity, and the effect of inhibitors.

108 tural evolution leading to improved or novel substrate specificities are not wholly defined.

109 mTORC1) and 2 (mTORC2), whose activities and substrate specificities are regulated by complex co-fact

110 xes, mTORC1 and mTORC2, whose activities and substrate specificities are regulated by complex specifi

111 ng that Parkin activation and acquisition of substrate specificity are coupled.

112 te a highly conserved structure, elements of substrate specificity are determined within the phosphat

113 Mechanisms of the altered 15-LO substrate specificity are enigmatic.

114 In contrast, defects in substrate specificity are not rescued in the double muta

115 s spectrometry, we unambiguously defined its substrate specificity as mono(ADP-ribosyl)ated aspartate

116 Substrate specificity assays performed with recombinant

117 Substrate specificity assays showed that LpdA hydrolyzed

118 the lid domain and beta5-loop contribute to substrate specificity but do not completely account for

119 ss to the adenosine pocket can contribute to substrate specificity but is not the sole determinant.

120 of the substrate-binding site, which confers substrate specificity by concerted conformational change

121 into subfamilies enhances the prediction of substrate specificity by phylogenetic analysis.

122 is conserved but suggests that the different substrate specificity can be related to replacement of a

123 The substrate specificity can now be rationalized and the st

124 of cancer, and numerous tumor proteases have substrate specificities compatible with proteolytic unma

125 ogether, this work suggests that a switch in substrate specificity coupled to the phosphorylation sta

126 T displayed significant differences in their substrate specificities, demonstrating that even modest

127 hat AA9 LPMOs can display different apparent substrate specificities dependent upon both productive p

128 different molecular scissors with different substrate specificities, depending on which of six Tspan

129 These results shed new light on substrate specificity determinants within the NPP enzyme

130 inding loop appears to be one contributor to substrate specificity differences between ADAR family me

131 al level of CDK kinase activity, rather than substrate specificity, drive the temporal ordering of S

132 ructural analysis, we could track changes in substrate specificity during ADP-dependent kinase evolut

133 Escherichia coli class Ia RNR with all four substrate/specificity effector-pairs bound (CDP/dATP, UD

134 hus, FATP1 and FATP4 likely have overlapping substrate specificities, enzymatic activities, and biolo

135 taphylococcus aureus, ClpP associates to the substrate specificity factors, ClpX and ClpC forming two

136 lation function and determined that PatB has substrate specificity for bioorthgonal short N-acetyl cy

137 T1 (SLC22A6) and OAT3 (SLC22A8) have similar substrate specificity for drugs, but it is far from clea

138 most informative and relevant for predicting substrate specificity for each individual kinase family.

139 Thereby we determined rate and substrate specificity for each single subunit without in

140 SbCCR1 and other CCRs likely confers strong substrate specificity for feruloyl-CoA over other cinnam

141 SbCYP82D1.1 has broad substrate specificity for flavones such as chrysin and a

142 AtProRS-Cyt showed nearly identical substrate specificity for L-proline and A2C, but for AtP

143 otif has been identified and used to explain substrate specificity for PKCalpha, it does not inform t

144 This prenyltransferase exhibits strict substrate specificity for stilbenoids and does not preny

145 riable F-box protein subunit that determines substrate specificity for ubiquitination.

146 anisms in Dcp2 also result in a shift of the substrate specificity from bacterial to eukaryotic mRNA.

147 how how gene loss can drive the evolution of substrate specificity from retained enzymes.

148 ate scope identical to AmbO5, but an altered substrate specificity from the wild-type enzymes.

149 as a means by which LSD1 acquires selective substrate specificities (H3K9 versus H3K4) to differenti

150 Furthermore, EYA1's substrate specificity has remained elusive.

151 molecular mechanism that underlies this dual substrate specificity has remained unknown.

152 tion, in terms of both overall chemistry and substrate specificity, have been brought about by signif

153 ry forces that cause an enzyme to change its substrate specificity; however, these processes remain l

154 dentity with IMPases, is responsible for the substrate specificity (i.e. allows the enzyme to disting

155 the transition in lectin ligand and esterase substrate specificity; (ii) in consequence, the switch i

156 Currently used MTGs have low substrate specificity, impeding their biotechnological u

157 lling is consistent with data on end-joining substrate specificity in cells, and provides insights in

158 nt was active in vitro but displayed altered substrate specificity in cultured cells, consistent with

159 of the drivers of the evolution of divergent substrate specificity in enzymes with identical active s

160 lays a dramatic reversal of beta-elimination substrate specificity in favor of l-serine over the norm

161 nges may be a common mechanism for dictating substrate specificity in other enzymes with multiple sub

162 NPF6 proteins exhibit different substrate specificity in plants and regulate nitrate tra

163 ng mechanism is likely to be a source of the substrate specificity in plants.

164 al protein-tyrosine phosphatases can exhibit substrate specificity in vivo by combining intrinsic enz

165 annel may serve as major determinants of the substrate specificity, including a glutamate residue at

166 idopsis mutants confirmed their activity and substrate specificity, indicating that plants in the Poa

167 is known about how evolution of transporter substrate specificities is linked to emergence of substr

168 mide synthases with distinct but overlapping substrate specificities is responsible for generation of

169 Zea mays, suggesting that the difference in substrate specificity is a conserved feature of ProRS is

170 Substrate specificity is achieved by a cluster of three

171 Substrate specificity is broad and relies on the carbon

172 se desaturases reveals that their particular substrate specificity is conferred by amino acid residue

173 This approach is easily modifiable, as substrate specificity is conferred by an antibody domain

174 e accumulation of carotenoids, and its broad substrate specificity is consistent with this.

175 The same study proposed that the PPOs' substrate specificity is controlled by one specific amin

176 Our results improve our understanding of how substrate specificity is determined in amino acid transp

177 How LF achieves such restricted substrate specificity is not understood.

178 of cellular functions; however, the extended substrate specificity is still unknown for many of these

179 ode active antibacterial toxins that possess substrate specificity matching extant Tae proteins of th

180 action or subtle differences within the same substrate specificity, matching the hybrid character of

181 s within the same protein complex, and PARdU substrate specificity may be primarily determined by the

182 The evolution of variable substrate specificity may help explain the reduced dermo

183 ide vital information for understanding USP7 substrate specificity, no such data has been available t

184 tes of approximately 1-80 nt/min and exhibit substrate specificities of approximately 0.1-5-fold for

185 te structures helped shine light on the fine substrate specificities of biosynthetic enzymes and conf

186 rometry, allowing one to rapidly analyze the substrate specificities of chitosan hydrolases that can

187 throughout the staphylococci and explore the substrate specificities of each enzyme.

188 nner, which may explain the wide spectrum of substrate specificities of EcEndoV.

189 as novel biochemical tools to determine the substrate specificities of endogalactanases.

190 The different substrate specificities of GODZ and SERZ-beta in vivo we

191 Evolution of novel substrate specificities of HAD phosphatases shows no str

192 mutant mouse models to explore the collagen substrate specificities of individual members of the pro

193 lgorithms have been developed to predict the substrate specificities of nonribosomal peptide syntheta

194 ur results provide a molecular basis for the substrate specificities of the acyl-CoA oxidases and rev

195 e aglycones released varied depending on the substrate specificities of the pectinolytic preparation,

196 re experiments to compare the activities and substrate specificities of these effectors.

197 The DNA substrate specificities of these Nei-like enzymes imply

198 ng a radiolabel-free assay revealed distinct substrate specificities of three DGTTs: CrDGTT1 preferre

199 This shows that an ArM can improve the substrate specificity of a catalyst and, for the first t

200 The catalytic mechanism and substrate specificity of a major PAL from sorghum (Sorgh

201 k establishes the capability of changing the substrate specificity of a protease at many positions in

202 The broad substrate specificity of acyltransferase CT775 provides

203 racterize fully the enzymatic parameters and substrate specificity of ADIPORs.

204 The substrate specificity of AnGDH towards glucose was inves

205 The superior substrate specificity of AnGDH was also demonstrated in

206 To understand the substrate specificity of APN for the development of targ

207 The extended substrate specificity of CELA3A and CELA3B was comparabl

208 The catalytic mechanism and substrate specificity of cinnamoyl-CoA reductases from s

209 tor pair that can be used to investigate the substrate specificity of cysteine proteases, serine prot

210 To explain the differences in substrate specificity of desulpho (ds)-Gl SOTs and to un

211 The substrate specificity of dionain-1 was largely similar t

212 Elucidating the substrate specificity of each PRMT will promote a better

213 Here, we discover and rationalize the substrate specificity of Eco1 using mass spectrometry co

214 ility of this approach for investigating the substrate specificity of enzymes from complex natural pr

215 The substrate specificity of enzymes from natural products'

216 This unique substrate specificity of EutT has important physiologica

217 le, we study the origin, diversification and substrate specificity of fungal NCS1 transporters.

218 Here, we revisited the substrate specificity of FUT8 by examining its in vitro

219 The substrate specificity of FUT8 toward bi-antennary N-glyc

220 Here, we defined the extended substrate specificity of FXIIa and its close homologue f

221 fer from Cbl to the substrate as well as the substrate specificity of GenK.

222 We tested the substrate specificity of inhibition and the contribution

223 odification at the Lys-93 position may alter substrate specificity of lipid phosphate phosphatase pro

224 on the MCD structure, we were able to shift substrate specificity of MCD toward succinyl-CoA through

225 ptides and pulse-chase assays to examine the substrate specificity of mouse R-transferase.

226 cell viability, although the regulation and substrate specificity of Mre11 have been difficult to de

227 mutations of key residues indeed reverse the substrate specificity of OprO to OprP and support the vi

228 reement with the available literature on the substrate specificity of pancreatic lipase.

229 this assay is well adapted for studying the substrate specificity of PLD, together with its kinetic

230 Considering possible overlapping substrate specificity of PRMTs, 17 and 46 are valuable c

231 Characterizing the substrate specificity of protease enzymes is critical fo

232 Switching of the substrate specificity of protein tyrosine phosphatase N1

233 and asparagine-176 may account for the broad substrate specificity of PviPRX9.

234 Here, we determined the substrate specificity of rice (Oryza sativa) phytaspase

235 te the biochemical activity and nucleic acid substrate specificity of RnhA.

236 iew summarizes the structure, chemistry, and substrate specificity of sirtuins with a focus on how di

237 The robust substrate specificity of the APC/C prevents the potentia

238 Because the subcellular localization and substrate specificity of the catalytic subunit of protei

239 by a novel drug mechanism-modulation of the substrate specificity of the CRL4(CRBN) E3 ubiquitin lig

240 al mechanism that regulates the activity and substrate specificity of the cytochrome P450 ensemble th

241 s spectrometry was developed to identify the substrate specificity of the Drosophila melanogaster P-T

242 leotide substrate mimic explains the relaxed substrate specificity of the E. coli enzyme relative to

243 ts close homologues, possibly explaining the substrate specificity of the enzyme.

244 her protein complexes is responsible for the substrate specificity of the exosome.

245 a conceptual framework for understanding the substrate specificity of the F-box protein FBXO31 and th

246 ety of acyl substrates, we characterized the substrate specificity of the LovB methyltransferase (MT)

247 and that they have different effects on the substrate specificity of the same condensing enzyme.

248 A major difference was observed in the substrate specificity of the two enzymes.

249 These EZH2 mutants retained the substrate specificity of their predecessor complexes but

250 st diversity in metabolites and the matching substrate specificity of their transporters, little is k

251 Here we have further investigated the substrate specificity of these enzymes by characterizati

252 Understanding the substrate specificity of these enzymes could create oppo

253 In order to elucidate the mode of substrate specificity of these enzymes, we characterized

254 We characterize the substrate specificity of this evolved enzyme, revealing

255 lso presents the first information about the substrate specificity of two specific hyperthermophilic

256 or metabolic shift, mediated by differential substrate specificity of virus-encoded serine palmitoylt

257 st method for measuring the average rate and substrate specificity of XNA polymerases in a standard q

258 cture-based insights into the regulation and substrate specificity of ZAP-70, and then we review nove

259 NBDO and 2NTDO) to elucidate the enzyme- and substrate-specificity of C and H isotope fractionation.

260 lyses reveal that the N terminus governs the substrate specificity ofSdGluc5_26A.

261 n a soluble monovalent substrate and similar substrate specificities on a glycan array.

262 to cereblon (CRBN) to confer differentiated substrate specificity on the CRL4(CRBN) E3 ubiquitin lig

263 hanism of signal biosynthesis, the basis for substrate specificity, or the rationale for donor specif

264 ynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new bios

265 endopeptidases of disparate architecture and substrate specificity owing to several potential target

266 In this study we characterized the extended substrate specificity (P4-P1) of the NSP cathepsin G usi

267 Curiously, despite their mutually exclusive substrate specificities, PON1 and diisopropyl fluorophos

268 newly integrated CASSIS algorithm, improved substrate specificity prediction for non-ribosomal pepti

269 sfers GlcNDAz and, surprisingly, has altered substrate specificity, preferring to transfer GlcNDAz ra

270 well-characterized in terms of function and substrate specificity, regulation across most thioestera

271 A) site, but how these endonucleases achieve substrate specificity remains poorly understood.

272 Studies on the substrate specificity revealed that the alpha1,6-fucosid

273 n neurons, which was associated with altered substrate specificity, serves as a mechanism acquired by

274 OprP (Y62F and Y114D) led to an exchange in substrate specificity similar to OprO.

275 Previously published substrate specificity studies of BCO2 were conducted usi

276 s low-terpenoid clone, as well as assays for substrate specificity supported a biological role of CYP

277 ts of 12 Mg(2+)-dependent 3'-end RNases with substrate specificity that is mostly unknown.

278 ese two residues in generating the different substrate specificities, the double mutants were generat

279 Substrate specificity, the influence of pH and temperatu

280 hich amino acids in the kinase domain encode substrate specificity, the so-called determinants of spe

281 ted whether CELA3A and CELA3B evolved unique substrate specificities to compensate for the loss of CE

282 Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic path

283 ut inhibits it on others, thereby conferring substrate specificity to SMARCAL1 fork-remodeling reacti

284 e ketoreductase (KR) domain displays broader substrate specificity toward different beta-ketoacyl gro

285 th the idea that SUMOylation may govern PIM1 substrate specificity under certain contexts.

286 method for quantitatively describing kinase substrate specificity using an unbiased peptide library-

287 ease in proteolytic activity and a change in substrate specificity was observed with Cm-Tf.

288 How the switch in esterase substrate specificity was realized remained unresolved,

289 To characterize the determinants of substrate specificity, we have analyzed the crystal stru

290 itro Because NatA and Naa50 exhibit distinct substrate specificity, we propose that they modify diffe

291 tructural insights into the determination of substrate specificity were complemented by mutagenesis s

292 from various fungi, which exhibit different substrate specificities, were used to discriminate betwe

293 localization of gamma-secretases determines substrate specificity, while FAD-causing mutations stron

294 triple mutant that displays greatly altered substrate specificity with increased activity for the ox

295 65 A resolution, and we compare its in vitro substrate specificity with those of fungal Icp55 enzymes

296 ic methyltransferase with unprecedented dual substrate specificity within the seven beta-strand class

297 ortunity to study the molecular evolution of substrate specificity within the type I PKSs.

298 iple cyclin-CDK complexes reveals how cyclin-substrate specificity works alongside activity threshold

299 id and accurate prediction of their extended substrate specificity would also aid in the design of cu

300 , mutation of a single residue in IcmF tuned substrate specificity yielding an approximately 4000-fol