ライフサイエンスコーパス: subunit interface

1 e located near the lipid bilayer center (and subunit interface).

2 l group and GluN1-Glu781 at the GluN1-GluN2A subunit interface.

3 neral anesthetics bind at the betaM3-alphaM1 subunit interface.

4 se complexes and observe many changes at the subunit interface.

5 eine 111 (Cys111) located at the edge of the subunit interface.

6 nts of l-glutamic acid (l-Glu) bound to each subunit interface.

7 within the extracellular domains including a subunit interface.

8 the transmembrane domain at the gamma-alpha subunit interface.

9 binds at the noncanonical beta2(+)/alpha3(-) subunit interface.

10 e 3 trinuclear copper cluster at the subunit-subunit interface.

11 BZD binding pocket at the alpha(1)/gamma(2) subunit interface.

12 d by loops C and F on opposing faces of each subunit interface.

13 dues at positions 248 and 250 at the subunit-subunit interface.

14 tes for mutagenesis, because they are at the subunit interface.

15 s through the hydrophilic passage toward the subunit interface.

16 sitively charged cleft formed at the subunit-subunit interface.

17 r-195 of loop C and Met-116 of loop E at the subunit interface.

18 enzyme are located in clefts at the subunit/subunit interface.

19 hR, with higher affinity for the alpha/delta subunit interface.

20 carbohydrates are at or near the noncovalent subunit interface.

21 mutations in the electrostatic region of the subunit interface.

22 a closure of the GABA-binding cavity at the subunit interface.

23 originate from opposite sides of the subunit-subunit interface.

24 teractions with tRNA and perturbation of the subunit interface.

25 2-14' position is located in the alpha-gamma subunit interface.

26 otentiation requires an interaction across a subunit interface.

27 d to binding specifically at the alpha delta-subunit interface.

28 ith the ion conduction pathway formed at the subunit interface.

29 s indicating selectivity for the alpha/delta subunit interface.

30 trimer with a distinct cavity located at the subunit interface.

31 esidues that form tRNA binding sites and the subunit interface.

32 its binding site may be at the alpha + beta- subunit interface.

33 le positions and within a relatively "tight" subunit interface.

34 r located >35 A away across the alpha2:beta2 subunit interface.

35 s to encircle the 40S, occupying part of the subunit interface.

36 ing sites and at the noncanonical delta-beta subunit interface.

37 with the orthosteric sites at the beta/alpha subunit interface.

38 ds to the homologous site at the beta3-beta3 subunit interface.

39 near one ACh binding site at the alpha/gamma subunit interface.

40 distortion in an extended loop near the PCNA subunit interface.

41 structural models locate in the beta+/alpha- subunit interface.

42 erms of the energetics and geometry of the c-subunit interfaces.

43 Its agonists bind at extracellular subunit interfaces.

44 arry with them lipids, bound specifically in subunit interfaces.

45 , since the five binding sites reside at the subunit interfaces.

46 stabilizing noncovalent interactions across subunit interfaces.

47 lutionary considerations, 17 were located at subunit interfaces.

48 within the delta subunit helix bundle and at subunit interfaces.

49 ization with the active sites located at the subunit interfaces.

50 residues at all regions along the chain and subunit interfaces.

51 the catalytic sites located near the subunit-subunit interfaces.

52 uding extended polypeptides penetrating into subunit interfaces.

53 seven domain 2 residues at each of the three subunit interfaces.

54 n the hexamer channel and nucleotides at the subunit interfaces.

55 ATP sites (sites A, B, C, and D) located at subunit interfaces.

56 d, HAP18 is found at quasiequivalent subunit-subunit interfaces.

57 ite is formed by six loops that intersect at subunit interfaces.

58 ho hexamer that result in the opening of the subunit interfaces.

59 binding sites at alpha/delta and alpha/gamma subunit interfaces.

60 irectly proportional to the concentration of subunit interfaces.

61 s are well conserved, as well as residues at subunit interfaces.

62 its, form ligand-binding sites at alpha/beta subunit interfaces.

63 ed ionic and hydrophobic interactions on the subunit interfaces.

64 which three active sites are created at the subunit interfaces.

65 ls, were strikingly localized at specific IN subunit interfaces.

66 extracellular domain at the beta(+)-alpha(-) subunit interfaces.

67 sis defects tended to be highly localized at subunit interfaces.

68 d nAChR stoichiometries and alpha4/accessory subunit interfaces.

69 affecting iGluRs appear to bind to sites in subunit interfaces.

70 lectrostatic interactions that stabilize the subunit interfaces.

71 ompetitive antagonists are found at selected subunit interfaces.

72 r alphaepsilon (adult) or alphagamma (fetal) subunit interfaces.

73 alpha-gamma (fetal) or alpha-epsilon (adult) subunit interfaces.

74 the etomidate sites at the beta(+)-alpha(-) subunit interfaces.

75 which involves disruption of the DNA clamp's subunit interfaces.

76 ronal nAChR appear to occur at the non-alpha subunit interface, a site presumed to be similar to that

77 ms a toroidal structure but with distinctive subunit interfaces absent from the other SMC complexes;

78 ation of an enzyme-bound water molecule at a subunit interface (active site) using FTICR-MS.

79 BzF incorporation at subunit interfaces afforded photocrosslinking of subunit

80 ite, including some mapping to the FtsA-FtsA subunit interface, also enhanced ATP transactions and ex

81 s proton binding by marked reorganization of subunit interface, altering the packing of beta-sheets t

82 cts of the mutations on the structure of the subunit interface and dimer stability are quite distinct

83 reby showing that LF(N) spans the PA subunit:subunit interface and explaining why heptameric PA binds

84 photolabeled deltaTyr-212 at the delta-beta subunit interface and gammaTyr-105 in the vestibule of t

85 mportant for the structural stability of the subunit interface and glycine binding site, and its muta

86 consistent with it binding at the alpha/beta subunit interface and not at the ligand-binding site in

87 ocal conformational change that disrupts the subunit interface and results in global changes responsi

88 ed from molecular modeling of the alpha-beta subunit interface and suggests that in alpha3betaGlyRs,

89 ctively) reveal interactions deep within the subunit interface and the absence of a contact surface w

90 The position of W139 near a subunit interface and the active site cleft suggested th

91 s, to evaluate the importance of each at the subunit interface and to determine if monomeric enzymes

92 to identify residues located at the subunit-subunit interface and to determine the S-layer's topolog

93 ructures define the active site to be at the subunit interface and, as confirmed by steady-state kine

94 tes that binding to sites at the gamma-alpha subunit interface and/or within delta subunit helix bund

95 The subunit interfaces and crystallographic packing properti

96 amer with identical ACh binding sites at the subunit interfaces and show ligand specificities resembl

97 tations of all Mediator subunits, as well as subunit interfaces and some secondary structural element

98 bute to etomidate sites formed at alpha-beta subunit interfaces and that increasing side-chain bulk a

99 p that is covalently cross-linked across its subunit interfaces and thus rendered incapable of openin

100 nd at the surface; stems are enriched at the subunit interface, and junctions near the peptidyl trans

101 /alpha9 rather than the alpha9/alpha10 nAChR subunit interface, and may facilitate the development of

102 Each type of binding site occurs at a subunit interface, and mutations on either side of the i

103 ntiator of nAChRs containing analpha4:alpha4 subunit interface, and that its intrinsic photoreactivy

104 amate binds in the classical agonist site at subunit interfaces, and picrotoxin directly occludes the

105 hree active sites are located at the subunit-subunit interfaces, and the two dTDP-sugar ligands emplo

106 entify several interactions on both pairs of subunit interfaces, and within the alpha subunits, which

107 hexameric rings on DNA via transient gaps at subunit interfaces; and helicase makers, which assemble

108 Although the overall subunit interfaces appeared to be similar for PKA holoen

109 sid and a correctly folded amino-terminal CA subunit interface are essential for saturation of host r

110 wing the effects of side-chain alteration at subunit interfaces are also enzymatically characterised.

111 (ii) Several nAChR subunit interfaces are also occupied, in a site suggeste

112 Changes in the conformation of inter-subunit interfaces are central to transmission of ligand

113 e observed differences in loop topologies at subunit interfaces are consistent with the inability of

114 f Asn190 to Asn190 hydrogen bonds across the subunit interfaces are necessary for activity.

115 First, rather than a ferroxidase site at the subunit interface, as is observed in all other DPS prote

116 ter on the solvent-accessible surface of the subunit interface at a likely site of action for modulat

117 an allosteric binding site that forms at the subunit interface at the apex of the receptor.

118 teract with more than one actin subunit at a subunit interface at the barbed end.

119 cursor substrate bind specifically to the PS subunit interface, at a site close to the active site.

120 olabeling of amino acids in the beta3-alpha1 subunit interface (beta3Met-286 in beta3M3 and alpha1Met

121 ands occupy a cavity that extends toward the subunit interface between GluN1 and GluN2A ABDs.

122 This linker region is part of the subunit interface between monomers, and point mutations

123 ues as potential Cu(2+)-binding sites at the subunit interface between thumb subdomain of alphahENaC

124 modulators suggest that cholesterol binds to subunit interfaces between transmembrane domains of the

125 oactivated molecule was incorporated in each subunit interface binding site based on analysis of the

126 DNA) revealed ssDNA associating across tight subunit interfaces but not at the loose interfaces, indi

127 and complexes with AChBP show binding at the subunit interface, but with an orientation or binding po

128 N46 ensuring correct alignment of the alpha1 subunit interface by interaction with juxtaposed residue

129 ) must be splayed open at one of the subunit-subunit interfaces by the ATP-dependent clamp loader, Re

130 ocated within the active site but near large subunit interfaces can affect key kinetic properties of

131 lt bridges located at the cytoplasmic domain subunit interfaces (CD-Is) of eukaryotic Kir channels co

132 t reactivity of substituted cysteines at the subunit interface changes dramatically during GCK-3-medi

133 asmic C-terminus inhibits CLH-3b by inducing subunit interface conformation changes that activate the

134 enous anesthetics bind in the betaM3-alphaM1 subunit interface consistent with azi-etomidate photoaff

135 hat can be modeled well by an opening of the subunit interface consistent with P-cluster fragmentatio

136 uggest that the cluster of histidines at the subunit interface controls access of zinc to its binding

137 ses a global compaction of the extracellular subunit interface, coupled to an outward motion of the M

138 and carbamazepine stabilize the heteromeric subunit interface critical for channel biogenesis to ove

139 d activation determinants are located at the subunit interface defined by our etomidate site model.

140 ing to the pharmatope, inserted at a subunit-subunit interface diametrically distinct from the agonis

141 and of agonist binding at the alpha4:alpha4 subunit interface, did not reduce CMPI potentiation.

142 Mutation of the side chains at the subunit interface disrupted in vitro dimerization of bot

143 teine mutants revealed that the alpha7-beta2 subunit interface does not bind ligand in a functionally

144 d mutagenesis to examine the contribution of subunit interface domains to alpha9alpha10 receptors by

145 hat chemical crosslinking of cohesin's three subunit interfaces entraps sister DNAs of circular but n

146 terestingly, six of nine residues located at subunit interfaces exhibit altered allosteric properties

147 bases, whether in the binding slot or in the subunit interface, face the protein in a manner that is

148 -alkyne cycloaddition reactions at a dynamic subunit interface facilitated the synthesis of potential

149 by providing quantitative information on the subunit interfaces formed in protein associations.

150 it interfaces from the homomeric alpha GlyR, subunit interfaces from the heteromeric alphabeta GlyR h

151 Unlike subunit interfaces from the homomeric alpha GlyR, subuni

152 , in concert with the static AKAP-regulatory subunit interface, generates a solid-state signaling mic

153 Electrostatic interaction at the subunit interface greatly influences the dimer-monomer e

154 012, the inhibition of the polymerase PA-PB1 subunit interface has become an active field of research

155 hR subunit; and 2) a site at the gamma-alpha subunit interface, identified by photolabeling of gammaM

156 le surface area buried upon formation of the subunit interface in MPT synthase was estimated to be 23

157 are closely opposed across the RecA subunit-subunit interface in some recent models of the RecA nucl

158 n ATP hydrolysis in trans across the subunit-subunit interface in the RecA nucleoprotein filament.

159 6 forms across the substrate-binding subunit-subunit interface in the substrate-bound form of BsPFK.

160 virus in which helix bundling dominates the subunit interface in tobacco mosaic virus and conveys ri

161 idues K80, K277, R284, R285, and E388 at the subunit interface in transmission of the allosteric sign

162 revealed not only the central role played by subunit interfaces in iGluR function, but also myriad bi

163 acking vicinal cysteines (i.e. the non-alpha subunit interfaces in nAChRs).

164 experiments has led to a map of the subunit/subunit interfaces in the assembled tubes.

165 the PAS beta-sheet generates two alternative subunit interfaces in the dimer.

166 Ch), bind to an orthosteric site, located at subunit interfaces in the extracellular domain.

167 has fundamental implications for the role of subunit interfaces in the gating mechanism of P2X recept

168 at the alpha(+)-beta(-) and gamma(+)-beta(-) subunit interfaces in the transmembrane domain.

169 bular density, with at least three different subunit interfaces in this homopolymeric assembly.

170 s involving a non-redundant set of 551 inter-subunit interfaces in viral capsids from VIPERdb and 20,

171 ed that the mutation altered salt bridges at subunit interfaces, including regions important for subu

172 tability and heterogeneity in principle make subunit interfaces inconsistent targets for the immune r

173 en two TatC molecules, with one of the inter-subunit interfaces incorporating a functionally importan

174 ylcholine binding to an aromatic cage at the subunit interface induces a significant contraction of l

175 which comprise another previously identified subunit interface, inhibit DNA cleavage by monomeric but

176 Our understanding of subunit interface interactions has been limited by the l

177 It targets the beta subunit interface involved in the tubulin longitudinal s

178 pha6*-nAChR function and suggest that unique subunit interfaces involving the complementary rather th

179 se results also suggest that the gamma-alpha subunit interface is a binding site for Torpedo nAChR ne

180 Although the native subunit interface is important for dimer stability, urea

181 n unique to the eukaryotic enzymes, and this subunit interface is rearranged by the binding of glucos

182 glutamate residue (Glu-343), located at the subunit interface, is positioned to catalyze a nucleophi

183 ASL mutants: R194C and K246E are located at subunit interfaces, L311V is in the central helical regi

184 AChBP to demonstrate ligand interactions at subunit interfaces lacking vicinal cysteines (i.e. the n

185 n of wild-type MreB with MreB mutated in the subunit interface leads to formation of shorter MreB fil

186 s flanking the ligand occupation site at the subunit interface, loop C (residues 175-193) and loop F

187 at the localization of drug binding sites to subunit interfaces may be a feature not only for GABA an

188 and that tyrosine 535 of NR1, located in the subunit interface, modulates the rate of ion channel dea

189 inter-subunit charge repulsion and create a subunit interface more favorable for dimerization must b

190 ucture revealed that the catalytic RNase H2A subunit interfaces mostly with the RNase H2C subunit tha

191 om the agonist binding site, interferes with subunit interface movements critical for receptor activa

192 e, a residue ~20 A away from Gluex, near the subunit interface, moves from buried to solvent-exposed.

193 those in the etomidate site, are located at subunit interfaces near the synaptic side of the transme

194 toxin at the peripheral surface of the inter-subunit interface occluding the agonist-binding site.

195 otooxidation of FnY356 within the alpha/beta subunit interface occurs within the Marcus inverted regi

196 5)CX(20)CC) in the intracellular T1-T1 inter-subunit interface of a Kv4 channel.

197 At one subunit interface of AChBP, the side chain of Tyr-89 clo

198 CX546, indicating that some residues at the subunit interface of glutamate receptors play an importa

199 binding pocket localized in the GluN1-GluN2B subunit interface of NMDA receptor tetraheteromeric comp

200 substitution at residue 178, located at the subunit interface of PCNA.

201 R194 is located at the subunit interface of sfALR, close to the intersubunit di

202 lysable form of ATP, the distance across the subunit interface of the clamp does not increase as meas

203 3beta-HSD localizes His156 or Tyr156 in the subunit interface of the enzyme homodimer.

204 at the extracellular N-terminal alpha/gamma subunit interface of the GABA-A receptor (GABAR).

205 d the amino acids clustered in an unexplored subunit interface of the holoenzyme.

206 t the buried face of the helix lies near the subunit interface of the homodimer in the equilibrium st

207 heterodimer formation are located within the subunit interface of the homodimer, far removed from the

208 hat the adenosine moiety of ATP binds at the subunit interface of the homotetrameric enzyme and that

209 at T2AA bound to the surface adjacent to the subunit interface of the homotrimer of PCNA in addition

210 rough the transmembrane beta((+))/alpha((-)) subunit interface of the receptor, possibly targeting a

211 The overall subunit fold and the subunit-subunit interface of the SOD3 dimer are similar to the c

212 Yet, the structural organization and the subunit interface of these PR oligomers were unknown.

213 t the in situ reaction can take place at the subunit interfaces of an oligomeric protein and can thus

214 lly, this approach may be used to target the subunit interfaces of any multisubunit protein having a

215 es in loop C of AChBP to mimic the non-alpha subunit interfaces of neuronal nAChRs and other Cys loop

216 nding pocket of nAChRs, are conserved in all subunit interfaces of neuronal nAChRs, including those t

217 r, in a comprehensive study of both types of subunit interfaces of seven of the eight normal oxy huma

218 called arginine fingers, are located at the subunit interfaces of the complex, which also harbor the

219 r binding at the alpha/delta and alpha/gamma subunit interfaces of the nAChR, with higher affinity fo

220 ion of the "noncanonical" Cys residue across subunit interfaces of the Td SOR homodimer.

221 Amino acid mutations at subunit interfaces of the tetramer were observed to dras

222 e to displace the fluorescent probe from the subunit interface on CK2alpha1-335.

223 ptophan 55 (Trp-55), which is located at the subunit interface on the complementary surface of the or

224 3, respectively, near the top of the subunit-subunit interface on the minus side, and that Asn-173 is

225 Pathogenic variants involve the subunit interfaces or inter-domain hinges of C1r and C1s

226 alphaHis-20 or alphaHis-50 do not change the subunit interfaces or the heme pockets of the proteins.

227 pproach also allowed us to mutate individual subunit interfaces, or combinations thereof, on each iso

228 al differences in binding subsites, receptor subunit interfaces, or transmembrane regions.

229 ed that the compound binds at the alpha/beta subunit interface overlapping the ligand-binding site th

230 T7 helicase, when a nucleotide is bound at a subunit interface, Phe(523) is buried within the interfa

231 n betaM1, the amino acid in the alpha1-beta3 subunit interface photolabeled by R-[(3)H]mTFD-MPAB.

232 etomidate bound to a site at the gamma-alpha subunit interface, photolabeling alphaM2-10 (alphaSer-25

233 ontacts between HAMP helices 1 and 2' at the subunit interface play a critical role in modulating rec

234 3), positioned in a beta-hairpin loop at the subunit interface, plays a key role in coupling the hydr

235 Exchange broadening at the subunit interface points to a rapid equilibrium between

236 meric helicase shows Arg-522, located at the subunit interface, positioned to interact with the gamma

237 (N110D) and GABRB1(F246S) mutations at beta- subunit interfaces produced minor changes in whole cell

238 of the linker region, which forms the small subunit interface, produces a severe growth defect and a

239 Here we identify two small subunit interface proteins S12 and S13 that are essentia

240 129-loop region, which comprises one subunit-subunit interface, provides an explanation for the disru

241 0N, E180G, Y302C) mutations located at beta+ subunit interfaces reduced whole cell currents by decrea

242 fective in dimerization map to the predicted subunit interface region, based on the crystal structure

243 the extracellular gate) and Helix P (at the subunit interface) remove the inhibitory effect of the c

244 ive mutagenesis and disease mutations at the subunit interface result in time-dependent channel inact

245 ase enzymes in which the binding of ATP at a subunit interface results in large conformational change

246 The position of the sugars near the subunit interface rules out their direct involvement in

247 This variability in the subunit-subunit interface, seen at atomic resolution in crystals

248 ore, RNA editing sites, located at strategic subunit interfaces, shape AMPA-R assembly and traffickin

249 f energetic coupling between residues at the subunit interface showed that deltaL42 is functionally l

250 n interfaces has no effect, constraining the subunit interface significantly perturbs metal uptake bu

251 n the ion channel and to a gamma(+)-alpha(-) subunit interface site similar to its GABAAR intersubuni

252 n with high affinity to the gamma(+)-beta(-) subunit interface site with negative energetic coupling

253 r entirely reflects binding to alpha7/alpha7 subunit interface sites.

254 rprisingly modified two distinct and distant subunit interface sites: loop F Y164 of the complementar

255 human hemoglobins are known to have altered subunit interface strengths, those of the normal embryon

256 h a binding site located in an extracellular subunit interface suggest that modulation via an agonist

257 Nevertheless, analysis of subunit interfaces suggested the functional significance

258 ween alpha1(-26') and alpha1(19') across the subunit interface suggests an important role in receptor

259 The highly specific nature of the subunit interfaces suggests that these could be good tar

260 Clear portraits of the subunit interface surfaces for 16 S and 23 S rRNA were o

261 outer surface of the hexamer but ends at the subunit interface that also includes residue 115.

262 nd Arg (167)/Asp (217) pairs) located in the subunit interface that has the largest surface area.

263 ly positively charged environment within the subunit interface that is distinct from the active site.

264 tructural rearrangement in the transmembrane subunit interface that reduces the betaM3 to alphaM1 sep

265 ctions are evident in regions at or near the subunit interface that reflect association-induced confo

266 mer, we locate these residues to two subunit-subunit interfaces that alternate with the two acetylcho

267 tal structures reveals six different subunit-subunit interfaces that are filament-like, but each is d

268 span (TM1), whereas W240 and W251 lie at the subunit interfaces that create the cytoplasmic vestibule

269 nAsn 39, and deltaAsn 41, located at the two subunit interfaces that form the agonist binding sites,

270 KBr is likely due to charge screening at the subunit interface, the influence on the monomer-dimer eq

271 tation induced additional alterations at the subunit interfaces, the active site, the relative positi

272 ccur at the transmitter-binding sites, alpha-subunit interfaces, the alphaM1 helix and the gate.

273 Upon ligand binding at the subunit interfaces, the extracellular domain of the nico

274 their glycan-protein hybrid epitopes are at subunit interfaces, the resulting heterogeneity can lead

275 es possess in general several unique subunit-subunit interfaces.The basic building block of such an a

276 A(A)R transmembrane domain at the beta-alpha subunit interface; the etomidate analog [(3)H]azietomida

277 ysis of dTTP, Phe(523) moves from within the subunit interface to a more exposed position where it co

278 ore of the channel and perturb a hydrophobic subunit interface to stabilize a closed state of the cha

279 backbone hydrogen bond, and then across the subunit interface to the side chain of a functionally im

280 2) --> W(48) --> Y(356) in beta2, across the subunit interface to Y(731) --> Y(730) --> C(439) in alp

281 over right arrow Y(356) in beta2, across the subunit interface to Y(731) left arrow over right arrow

282 RRE utilizes the inherent plasticity of Rev subunit interfaces to guide the formation of a functiona

283 AuIB), or alpha3/beta2 (alpha-conotoxin-MII) subunit interfaces to perturb responses induced by nicot

284 data that the structure of the alpha1-gamma2 subunit interface undergoes agonist-specific conformatio

285 V2 bnAbs and V3 crown antibodies that engage subunit interfaces upon binding to unliganded Env.

286 sts that the inhibitory zinc is located at a subunit interface using Cys-219 and His-10 as ligands.

287 The effect of agonists at various subunit interfaces was determined by blocking homologous

288 Furthermore, the kinetics of RT across the subunit interface were directly assessed for the first t

289 lower affinity to a site at the gamma-alpha subunit interface where etomidate analogs bind that act

290 ic sites of PNP are located near the subunit-subunit interfaces where F159 is a catalytic site residu

291 hydrogen bond with sulfate ions buried at a subunit interface, which in porcine FBPase undergoes sig

292 dimer to create a cross-linked dimer at its subunit interface, which was normally active for in vitr

293 transmembrane domain at the beta(+)-alpha(-) subunit interfaces, which also contain the GABA-binding

294 turb the alpha(1)beta(1) and alpha(1)beta(2) subunit interfaces, while as expected, the tertiary stru

295 ge T4 clamp protein labeled across its three subunit interfaces with a fluorescence resonance energy

296 eutralizing the repulsive negative charge at subunit interfaces with significant differentiation of q

297 ofol-selective binding within GABAA receptor subunit interfaces, with stable hydrogen bonds observed

298 Furthermore, we show by perturbing subunit interfaces within complexes and by mass spectrom

299 erved arginine fingers in PAN located at the subunit interface work together as a single allosteric u

300 tations of the redox-active tyrosines at the subunit interface, Y356F(beta) and Y731F(alpha), this ox