ライフサイエンスコーパス: sulfhydryl

1 e that specifically labels reactive cysteine sulfhydryls.

2 ally deglutathionylate cysteines and restore sulfhydryls.

3 1.Tb, possessing a maleimide moiety, as its sulfhydryl acceptor, was poorly emitting in aqueous pH 7

4 ify proximal relationships of the introduced sulfhydryls across the proposed interdomain interface.

5 t1 was resistant to the inhibitory effect of sulfhydryl-alkylating reagents, N-ethylmaleimide inhibit

6 ective capture method promoted enrichment of sulfhydryl analytes and reduced matrix interferences, th

7 ed mesh substrates were then used to capture sulfhydryl analytes directly from urine and plasma sampl

8 containing structural motifs such as selenyl-sulfhydryl and diselenide bonds.

9 irming the critical requirement of both free sulfhydryl and galactosamine moieties for inhibition of

10 l mice, with greater depletion of nonprotein sulfhydryl and occurrence of cytotoxicity (observable by

11 wo coordination sites filled by the cysteine sulfhydryls, and another by the amide nitrogen of Phe (3

12 ical activities of 6-DHSG were attenuated by sulfhydryl antioxidants such as glutathione (GSH) or N-a

13 eveloped to determine the percentage of free sulfhydryl at each cysteine residue of four recombinant

14 s glucose to the growth medium (termed 'High Sulfhydryl Bacillus subtilis' or HSBS) was compared to t

15 ncentration of sulfhydryl sites (termed 'Low Sulfhydryl Bacillus subtilis' or LSBS) and to sorption o

16 These data support the evaluation of sulfhydryl-based Id-KLH vaccines in lymphoma clinical tr

17 A sulfhydryl-based tumor Ag-carrier protein conjugation sy

18 could be abolished by the membrane-permeable sulfhydryl blocker, N-ethylmaleimide, by the RGD peptide

19 However, in this case a sulfhydryl bond is cleaved to release the lipophilic cyt

20 es surfaces through the formation of Ag(+I)--sulfhydryl bonds.

21 istances of 2.24-2.34 A were consistent with sulfhydryl-bound As(III).

22 res due to their failure to form an apparent sulfhydryl bridge, and they are retained by the QCS.

23 al redox-sensing properties through reactive sulfhydryl chemistry.

24 e methylene group with a nitrogenous base or sulfhydryl compound.

25 sediment were present as Ag(2)S (55%) and Ag-sulfhydryl compounds (27%).

26 such as aniline and veratrylamine as well as sulfhydryl compounds such as l-cysteine and beta-mercapt

27 al from Pseudomonas putida, and the measured sulfhydryl concentrations on bacterial EPS molecules are

28 This study suggests that the sulfhydryl concentrations on EPS molecules may vary sign

29 Sulfhydryl-containing compounds, including thiols and hy

30 effectively separate the reactivity of these sulfhydryl-containing compounds.

31 highlight the importance of modifications by sulfhydryl-containing ligands that can drastically influ

32 rapid response (<1 min) and is selective for sulfhydryl-containing nucleophiles over other reactive s

33 processing characterized by the loss of free sulfhydryl content (Fas-SH) and resultant increases in S

34 ment decreased the alpha-helix content, free sulfhydryl content, and Rg, it increased the random coil

35 Salt-soluble protein, surface reactive sulfhydryl content, and surface hydrophobicity of Alaska

36 ance, particle size, protein fractions, free sulfhydryl content, immunoreactivity, viscosity, gelling

37 ance, particle size and microstructure, free sulfhydryl content, protein fractions, protein electroph

38 rease in surface hydrophobicity and reactive sulfhydryl content; structurally, no clear denaturation

39 dies showed that the gamma subunit disulfide/sulfhydryl couple in the modified ATP synthase has a mor

40 ectrospray ionization-MS/MS as follows: 1) a sulfhydryl cross-link between C3.53(134) in TMH3 and the

41 using nonreduced polyacrylamide gels and the sulfhydryl cross-linker BMH.

42 Mia40 and the sulfhydryl:cytochrome c oxidoreductase Erv1/ALR are esse

43 aIIbbeta3 and alphavbeta3 and suggest a free sulfhydryl-dependent regulatory role for Cys-560-Cys-583

44 quantum efficiencies (Q(u)) of the amino and sulfhydryl derivatives were about an order of magnitude

45 tructural changes, arising from disulfide or sulfhydryl-disulfide bond-mediated aggregation of whey p

46 tributed to the significant reduction in the sulfhydryl-disulfide interchange reaction during denatur

47 with dithiothreitol indicated occurrence of sulfhydryl-disulfide interchange reactions.

48 Given our previous identification of sulfhydryl/disulfide redox status as a factor in photore

49 rent antibodies had different levels of free sulfhydryl due to multiple unpaired cysteine residues co

50 Furthermore, free sulfhydryl due to unpaired cysteine residues in the vari

51 chemical modification and inhibition of the sulfhydryl enzyme, glyceraldehyde-3-phosphate dehydrogen

52 cycle resulted in the catalytic oxidation of sulfhydryls even with nanomolar concentrations of seleni

53 he preference is for the anionic form of the sulfhydryl; for formamide, the neutral form is preferred

54 jor site of HSA adduction is the single free sulfhydryl group at Cys34, we used thiol-affinity resins

55 of single stranded DNA (ssDNA) probe through sulfhydryl group at the 5' phosphate end.

56 ealed that this mutation made the regulatory sulfhydryl group energetically much more difficult to re

57 tain a more stable form for oral dosing, the sulfhydryl group in conjugate 1 was converted into a fun

58 nges, specific for thiamine and sensitive to sulfhydryl group inhibition.

59 ine-persulfide (Cys-SSH) is a cysteine whose sulfhydryl group is covalently bound to sulfur (sulfane

60 yltriglycine with S-acetyl protection of the sulfhydryl group may be used to conjugate MAG3 to primar

61 Besides the sulfhydryl group of a cysteine, we make use of an azido

62 minal carbon of the acetylene moiety and the sulfhydryl group of Cys-803 at the solvent interface.

63 The reactive sulfhydryl group of Cys106 projects toward position C-4a

64 e presence of monovalent copper ions and the sulfhydryl group of Cys466.

65 olution containing NEM which reacts with the sulfhydryl group of GSH, thus locking the active form in

66 ilic attack of either the amine group or the sulfhydryl group of the substrate on the internal aldimi

67 t protein damage, characterised by the total sulfhydryl group reduction.

68 pproach, a cemadotin derivative containing a sulfhydryl group results in a mixed disulfide linkage.

69 enzymes that generate a product with a free sulfhydryl group, including histone acetyltransferases,

70 cysteine, which adds to the cofactor via its sulfhydryl group, possibly forming a cyclic thiazolidine

71 ng advantage of the reactivity of cysteine's sulfhydryl group, we modified these mutants with chemica

72 ddition or subtraction of a hydrogen atom or sulfhydryl group.

73 nt of an NO moiety to a nucleophilic protein sulfhydryl group.

74 lve a reversible 1,4-addition with a protein sulfhydryl group.

75 e protein, thioredoxin, between two cysteine sulfhydryl groups (i.e., staple), followed by photochemi

76 Binding of arsenite (As(III)) to sulfhydryl groups (Sorg(-II)) plays a key role in As det

77 res higher than 200 MPa, a decrease in total sulfhydryl groups and an increase in surface hydrophobic

78 ary lipid oxidation products and the loss in sulfhydryl groups and Ca(2+)-ATPase activity.

79 ified the GR protein by decreasing available sulfhydryl groups and decreasing nuclear GR expression a

80 It triggers a rapid covalent destruction of sulfhydryl groups and disulfide bonds via the mechanism

81 tein carbonyl content, oxidised amino acids, sulfhydryl groups and immuno-blotting against carbonyl g

82 The level of accessible free sulfhydryl groups and the surface hydrophobicity of unfo

83 arsenite; and d) As has a high affinity for sulfhydryl groups and therefore binds to GSH and Cys.

84 The sulfhydryl groups at the end of the molecular wire form

85 ate to cysteine, most likely indicating that sulfhydryl groups at these positions are appropriately s

86 ns that favored closure, indicating that the sulfhydryl groups come close enough to each other or to

87 nd B2 remained unchanged, while free exposed sulfhydryl groups decreased.

88 The presence of free sulfhydryl groups in five recombinant monoclonal antibod

89 duction-oxidation modifications of cysteinyl sulfhydryl groups in mature ADAM17 may serve as a mechan

90 can be used to quantify reactions targeting sulfhydryl groups in proteins.

91 The amount of titrated sulfhydryl groups in the protein concentration range inv

92 the reductase activity is unique in that no sulfhydryl groups in the YfcG protein are covalently inv

93 sted that the presence of low levels of free sulfhydryl groups is likely a common feature of recombin

94 s a T-rich probe DNA at one vertex and three sulfhydryl groups modified with 10nm Au NPs at the other

95 Reaction of MBB with the sulfhydryl groups of free cysteines leads to formation o

96 Arsenic binding by sulfhydryl groups of natural organic matter (NOM) was re

97 ecently, the complexation of trivalent As by sulfhydryl groups of NOM was proposed as the main mechan

98 hat these methods had a high selectivity for sulfhydryl groups on this protein, which accounted for t

99 on in vitro and decreased the number of free sulfhydryl groups on tubulin cysteines.

100 in gold or self-assembled monolayer via the sulfhydryl groups present in the hinge region.

101 n of complexes involving mercury species and sulfhydryl groups present in tissues and/or loss of wate

102 Reversibly oxidized cysteine sulfhydryl groups serve as redox sensors or targets of r

103 oyl cofactor, which is the attachment of two sulfhydryl groups to C6 and C8 of a pendant octanoyl cha

104 nd the use of a molecular wire terminated in sulfhydryl groups to connect the two modules.

105 the selective oxidation of protein cysteine sulfhydryl groups to disulfide bonds we examined the spe

106 The locations of the free sulfhydryl groups were determined using mass spectrometr

107 plasmin activity, prolidase level, and total sulfhydryl groups were evaluated.

108 Free sulfhydryl groups were first modified using 5-idoacetami

109 Redox-dependent modifications of sulfhydryl groups within the two Cys4 zinc fingers of th

110 ), has no other active redox moieties (e.g., sulfhydryl groups) and can exist in three different oxid

111 and Msh2-Msh6 interactions involve cysteine sulfhydryl groups, and the high Cd(2+):Msh2-Msh6 ratio i

112 x1, which was mainly fully reduced with five sulfhydryl groups.

113 isulfide bridges with cysteine or methionine sulfhydryl groups.

114 fluorescence detection of peptides with free sulfhydryl groups.

115 proteins and other ligands to silica through sulfhydryl groups.

116 the dynamic chemistry played by wine protein sulfhydryl groups.

117 sured were the levels of prolidase and total sulfhydryl groups.

118 of Ag(+) and/or Cd(+2) with the substituted sulfhydryl groups.

119 ated As was present as trivalent As bound by sulfhydryl groups.

120 Disruption of sulfhydryl homeostasis, which resulted in ER stress, acc

121 DOPA) which is a pro-oxidant and may disrupt sulfhydryl homeostasis.

122 proteins tend to have higher proportions of sulfhydryl, hydroxyl and acylamino, but lower of sulfide

123 ree sulfhydryl, similar distribution of free sulfhydryl in the domain structures was observed in the

124 of p65-NFkappaB to DNA, suggesting that most sulfhydryls in p65-NFkappaB protein were in reduced and

125 dely used reagent for the alkylation of free sulfhydryls in proteomic experiments.

126 ugh a redox modification of vicinal cysteine sulfhydryls in the catalytic domain of PKC.

127 onality with biological nucleophilic groups (sulfhydryl, indole, phenol, imidazole, carboxamide) that

128 Oxidation of crystallin methionine and sulfhydryls into sulfoxides was dramatically increased,

129 te covalently modifies beta2M286C side-chain sulfhydryls, irreversibly altering GABA-induced currents

130 A low percentage of free sulfhydryl is a common feature of recombinant monoclonal

131 In the present study we performed in vivo sulfhydryl labeling of an extensive collection of monocy

132 ombination of LacZ/PhoA reporter fusions and sulfhydryl labelling by PEGylation of novel cysteine res

133 oteins, resulting in overall decreased total sulfhydryl levels.

134 Our results thus document that sulfhydryl ligands are highly competitive As(III) comple

135 The ability of sulfhydryl ligands to compete with ferrihydrite for As(I

136 polyethylene glycol-2000], are conjugated to sulfhydryl lipids via maleimide reactive groups on the Q

137 gation between the cross-linked peptides and sulfhydryl magnetic beads by analyzing supernatant solut

138 confirming that peptides can be isolated on sulfhydryl magnetic beads by using Sulfo-LC-SPDP.

139 ormation of Sorg(-II)-As(III) complexes on a sulfhydryl model adsorbent (Ambersep GT74 resin) in the

140 We also measured rates of sulfhydryl modification by p-chloromercuribenzenesulfona

141 GABA altered the rates of sulfhydryl modification of alpha1K219C, beta2K213C, and

142 This was substantiated by site-directed sulfhydryl modification of the proton glutamate mutant E

143 Sulfhydryl modification of Y124C by 2-aminoethyl methane

144 Here, we examined sulfhydryl modifications of the p65 subunit of NFkappaB

145 arrying out selective desulfurization of the sulfhydryl-modified sugar moiety in the presence of acet

146 l methanethiosulfonate (a membrane-permeable sulfhydryl modifier)-mediated enhancement of the binding

147 ctivity was sensitive to N-ethylmaleimide, a sulfhydryl-modifying reagent.

148 probed with a series of methanethiosulfonate sulfhydryl-modifying reagents.

149 kedly enhanced the accessibility of cysteine sulfhydryl moieties in DAT as probed by a membrane-imper

150 hydroxyl group or conversion of a methyl or sulfhydryl moiety to a hydroxyl, can confer modified Ag-

151 n are two highly conserved, vicinal cysteine sulfhydryl motifs (cysteine-X-X-cysteine), which are wel

152 tissue antioxidants [estimated by nonprotein sulfhydryl (NPSH) levels] and/or induction of oxidant st

153 The incorporation of a sulfhydryl nucleophile into a phosphoinositide hapten de

154 n between isothiocyanate of sulforaphane and sulfhydryl nucleophiles of Keap1 is kinetically labile,

155 isulfide regenerates the reduced active-site sulfhydryl of 1-Cys Prx.

156 s study implicates selective modification of sulfhydryls of target proteins in some of the cytotoxic

157 re/function and/or prevents degradation from sulfhydryl overoxidation or proteolysis.

158 es the substrate specificity of the quiescin sulfhydryl oxidase (QSOX) family of disulfide-generating

159 The quiescin sulfhydryl oxidase (QSOX) family of enzymes generates di

160 ge) showed it to be a member of the Quiescin-sulfhydryl oxidase (QSOX) family.

161 Quiescin sulfhydryl oxidase (QSOX) flavoenzymes catalyze the dire

162 The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into

163 The flavoprotein quiescin-sulfhydryl oxidase (QSOX) rapidly inserts disulfide bond

164 tein containing the ERV/ALR domain, quiescin-sulfhydryl oxidase (QSOX).

165 , PRDX4, and the candidate oxidants quiescin-sulfhydryl oxidase 1 (QSOX1) and vitamin K epoxide reduc

166 sion (SAGE) databases was enzyme quiescin Q6 sulfhydryl oxidase 1 (QSOX1).

167 ing that the C-X-X-C motif was essential for sulfhydryl oxidase activity and responsible for the alte

168 92 C(155)XXC(158) amino acids, important for sulfhydryl oxidase activity, were mutated to A(155)XXA(1

169 luble 62 kDa FAD-linked and EDTA-insensitive sulfhydryl oxidase apparently constitutes the dominant d

170 ysteine and selenomethionine residues in the sulfhydryl oxidase augmenter of liver regeneration (ALR)

171 and depend on the oxidoreductase Mia40, the sulfhydryl oxidase augmenter of liver regeneration (ALR)

172 The sulfhydryl oxidase augmenter of liver regeneration (ALR)

173 Reoxidation of Mia40 is facilitated by the sulfhydryl oxidase Erv1 and the respiratory chain.

174 The sulfhydryl oxidase Erv1 partners with the oxidoreductase

175 ctions involving disulfide transfer from the sulfhydryl oxidase Erv1 to Mia40 and from Mia40 to subst

176 us pathway with the oxidoreductase Mia40 and sulfhydryl oxidase Erv1, termed the mitochondrial interm

177 hen in excess or at a lower rate by only the sulfhydryl oxidase Erv1.

178 t that Tim17 can be directly oxidized by the sulfhydryl oxidase Erv1.

179 Erv1 is a flavin-dependent sulfhydryl oxidase in the mitochondrial intermembrane sp

180 with the augmenter of liver regeneration, a sulfhydryl oxidase of the mitochondrial intermembrane sp

181 neration (ALR) is both a growth factor and a sulfhydryl oxidase that binds FAD in an unusual helix-ri

182 1-like (Gfer) is an evolutionarily conserved sulfhydryl oxidase that is enriched in embryonic and adu

183 The first mammalian sulfhydryl oxidase to be described was an iron-dependent

184 family of flavin adenine dinucleotide-linked sulfhydryl oxidases and is related to the ERV/ALR family

185 Both metal and flavin-dependent sulfhydryl oxidases catalyze the net generation of disul

186 These metazoan sulfhydryl oxidases have four recognizable domains: a re

187 AD prosthetic group of the ERV/ALR family of sulfhydryl oxidases is housed at the mouth of a 4-helix

188 ies between members of the ERV/ALR family of sulfhydryl oxidases provides insights into their likely

189 ases and is related to the ERV/ALR family of sulfhydryl oxidases.

190 on to the earlier reports of metal-dependent sulfhydryl oxidases.

191 redox capacity and reduces cellular protein sulfhydryl oxidation and, in particular, oxidation of mi

192 To gain insight into the requirement for sulfhydryl oxidation during virus replication, a virus w

193 t increased Prx expression and resistance to sulfhydryl oxidation in Abeta-resistant nerve cells is a

194 Conversely, increased intracellular heme or sulfhydryl oxidation inactivate BACH1, permitting transc

195 The documentation of a sulfhydryl-oxidizing activity in the thylakoid lumen fur

196 M), a pharmacologically active member of the sulfhydryl proteolytic enzyme family, obtained from Anan

197 containing alpha-beta unsaturated carbonyls, sulfhydryl reactive metals, and isothiocyanates are stro

198 beta unsaturated carbonyls, isothiocyanates, sulfhydryl reactive metals, flavones, and polyphenols.

199 The sulfhydryl reactive reagent 2-(trimethylammonium)ethyl m

200 Intracellular application of the sulfhydryl reactive reagent MTSET using CLH-3b channels

201 a7 ligand-binding domain allowing us to bind sulfhydryl-reactive (SH) agonist analogs or control reag

202 in CHO cells and covalently labeled with the sulfhydryl-reactive fluorophore monobromo-trimethylammon

203 method, which utilizes cysteine residues and sulfhydryl-reactive nitroxide reagents, can be challengi

204 ly oxidize SCN(-) to generate HOSCN, a weak, sulfhydryl-reactive oxidant, as a major physiologic prod

205 The sulfhydryl-reactive oxidative agent diamide suppressed L

206 s-linking screen using the photoactivatable, sulfhydryl-reactive reagent N-[4-(p-azidosalicylamido)bu

207 modification of the introduced cysteines by sulfhydryl-reactive reagents in the absence and presence

208 issue by utilizing our previously developed sulfhydryl-reactive, cleavable, radioiodinated photocros

209 eptors expressed in Xenopus oocytes with the sulfhydryl-reactive, environmentally sensitive fluoresce

210 d their accessibility to modification by the sulfhydryl reagent 3-(N-maleimido-propionyl) biocytin (M

211 hione (GSH) involves oxidation of GSH by the sulfhydryl reagent 5,5'-dithio-bis(2-nitrobenzoic acid)

212 essibility of introduced Cys residues to the sulfhydryl reagent MTSET.

213 es for alpha7-W55C nAChRs, whereas a neutral sulfhydryl reagent potentiated responses; residue C55 wa

214 -less CLH-3b mutant, we demonstrate that the sulfhydryl reagent reactivity of substituted cysteines a

215 harge restoration using a negatively charged sulfhydryl reagent reinstated also the WT phenotype.

216 Exposure to oxidative stress via the sulfhydryl reagent thimerosal resulted in a greater decr

217 bition of transport by a membrane impermeant sulfhydryl reagent was diminished under conditions expec

218 endogenous cysteine to a membrane impermeant sulfhydryl reagent was enhanced by the D451E mutation, s

219 us cysteine residue to a membrane-impermeant sulfhydryl reagent was increased relative to wild type,

220 vity of the mutants to a membrane-impermeant sulfhydryl reagent was not conformationally sensitive.

221 n externally applied and membrane-impermeant sulfhydryl reagent.

222 by p-chloromercuriphenylsulfonate (pCMPS), a sulfhydryl reagent.

223 Cytoplasmically applied membrane-impermeant sulfhydryl reagents alter the Ca2+ sensitivity of Ano1 E

224 easily accessible to extracellularly applied sulfhydryl reagents and select for anionic sulfhydryl re

225 te their sensitivity to membrane-impermeable sulfhydryl reagents as exchanger current block.

226 Several positively charged sulfhydryl reagents blocked ACh-induced responses for al

227 d sulfhydryl reagents and select for anionic sulfhydryl reagents over cationic ones.

228 However, both sulfhydryl reagents strongly inhibited the L750C mutant

229 ed to the loss of the ability of the charged sulfhydryl reagents to inhibit NBMPR binding in isolated

230 However, the capacity of charged sulfhydryl reagents to inhibit the binding of NBMPR in b

231 ha7-W55C) and testing the ability of various sulfhydryl reagents to react with this cysteine.

232 th membrane-permeant and membrane-impermeant sulfhydryl reagents under a variety of conditions.

233 mino)ethyl methanethiosulfonate (MTS-TAMRA)) sulfhydryl reagents, 4 with only BM, and 3 with only MTS

234 robed their state-dependent accessibility to sulfhydryl reagents.

235 ive to vanadate, Ca(2+), and modification by sulfhydryl reagents.

236 nd cumene hydroperoxide, and is inhibited by sulfhydryl reagents.

237 de of TM3, was affected by membrane-permeant sulfhydryl reagents.

238 Thus, sulfhydryl redox modification can regulate various aspec

239 This enhancement was blocked by sulfhydryl reducing agents, demonstrating a reversible m

240 le manner, by treatment of the channels with sulfhydryl reducing agents, suggesting that it was mostl

241 In contrast, sulfhydryl reduction had limited effects on channel open

242 r that can be reversibly coupled to a target sulfhydryl residue via a disulfide bond.

243 Free sulfhydryl, resulting from the reduction of disulfide bo

244 Conjugation of monomeric and dimeric sulfhydryl-RGD peptides with 18F-FBEM was achieved in hi

245 othelial surfaces, NO is associated with the sulfhydryl-rich protein tissue transglutaminase (TG2), t

246 Nearly all the hits from our recent sulfhydryl-scavenging high-throughput screen (HTS) targe

247 This study highlights the necessity of sulfhydryl (SH) groups in maintaining the structural int

248 e predicted based on the measurement of free sulfhydryl (-SH) content on applying mildly denaturing c

249 are involved in disulfide bonds, and no free sulfhydryl should be detected.

250 eagent, suggesting that interactions between sulfhydryl side chains of IDH2 Cys-150 residues limit ac

251 fferent locations in FepA and modified their sulfhydryl side chains with fluorescein maleimide in liv

252 ntibodies contained different levels of free sulfhydryl, similar distribution of free sulfhydryl in t

253 ose concentrations yielding higher bacterial sulfhydryl site concentrations for each species studied.

254 concentration does not significantly affect sulfhydryl site concentrations for S. oneidensis and P.

255 o the TSB medium significantly increases the sulfhydryl site concentrations for the three Bacillus sp

256 Our results suggest that bacterial sulfhydryl site concentrations in natural systems are li

257 ium enriched with 50 g/L of glucose, and the sulfhydryl site concentrations of the obtained biomass s

258 ss samples were determined through selective sulfhydryl site-blocking, potentiometric titrations, and

259 subtilis biomass with a low concentration of sulfhydryl sites (termed 'Low Sulfhydryl Bacillus subtil

260 emoval, indicating that virtually all of the sulfhydryl sites are located on the EPS molecules produc

261 Bacterial sulfhydryl sites can form strong complexes with chalcoph

262 These results suggest that bacterial sulfhydryl sites control the sorption behavior of these

263 ed in order to determine the distribution of sulfhydryl sites on bacteria.

264 In this study, the concentration of sulfhydryl sites on bacterial biomass samples with and w

265 is crucial to quantify the concentration of sulfhydryl sites on EPS molecules of other bacterial spe

266 mainly due to the elevated concentration of sulfhydryl sites on the bacteria.

267 biomass with induced high concentrations of sulfhydryl sites represents a promising and low cost bio

268 PS produced by S. oneidensis contained fewer sulfhydryl sites than those present on the untreated cel

269 media strongly affects the concentration of sulfhydryl sites that are present on the bacteria, with

270 After blocking the bacterial cell envelope sulfhydryl sites using a qBBr treatment, the sorption of

271 ida samples was 34.9 +/- 9.5 mumol/g, and no sulfhydryl sites were detected after EPS removal, indica

272 Prior to EPS removal, the measured sulfhydryl sites within P. putida samples was 34.9 +/- 9

273 In contrast, the sulfhydryl sites within the S. oneidensis samples increa

274 is biomass with an elevated concentration of sulfhydryl sites, induced by adding excess glucose to th

275 ce Cys residues were further modified by the sulfhydryl-specific alkylating agent, 5-fluorescein-male

276 Furthermore, rapidly reacting, sulfhydryl-specific chemical cross-linkers, methanethios

277 Here, sulfhydryl-specific cross-linking strategy was employed

278 hannels in Xenopus oocytes, and used in vivo sulfhydryl-specific crosslinking to directly examine the

279 ution, allowing filaments to be labeled with sulfhydryl-specific fluorescent dyes.

280 ility studies using the membrane-impermeant, sulfhydryl-specific methanethiosulfonate reagents.

281 f SNAP on N-type currents was blocked by the sulfhydryl-specific modifying reagent methanethiosulfona

282 of oocytes in the presence of the impermeant sulfhydryl-specific reagent, p-chloromercuribenzene sulf

283 bility method using the membrane-impermeant, sulfhydryl-specific reagent, p-chloromercuribenzene-sulf

284 bility method using the membrane-impermeant, sulfhydryl-specific reagent, p-chloromercuribenzenesulfo

285 ulfide linkage with the active site cysteine sulfhydryl specifically.

286 Surface reactive sulfhydryl (SRSH) contents of the three fish species beh

287 rmine whether a cysteine is reduced (free in sulfhydryl state), half-cystine (involved in a disulfide

288 Iron affects the sulfhydryl status of Yap5, which is indicative of the ge

289 Surface hydrophobicity, sulfhydryl status, secondary structure profile, differen

290 avenger capacity) and cellular antioxidants (sulfhydryl, superoxide dismutase) of zebrafish brain wer

291 Protein sulfhydryls that had undergone H2O2 mediated glutathiony

292 e concentrations of GSH and other nonprotein sulfhydryls through binding and irreversible loss in bil

293 information correlating the presence of free sulfhydryl to specific cysteine residues.

294 roxy for dimethylamino (DMAQ and DMAQ-Cl) or sulfhydryl (TQ) significantly alters the photochemical a

295 tion of isolates among cefotaximase (CTX-M), sulfhydryl variable, and temoneira enzymes and data on p

296 A low percentage of free sulfhydryl was detected at every cysteine residue in the

297 Free sulfhydryl was first alkylated with 12C iodoacetic acid.

298 Interestingly, free sulfhydryl was rarely associated with cysteine residues

299 The percentage of free sulfhydryl was then calculated using the two m/z series

300 Cysteine sulfhydryls within CCTalpha are needed for full catalyti