ライフサイエンスコーパス: sulfhydryl group

1 ent product results upon modification of the sulfhydryl group.

2 ddition or subtraction of a hydrogen atom or sulfhydryl group.

3 nt of an NO moiety to a nucleophilic protein sulfhydryl group.

4 lve a reversible 1,4-addition with a protein sulfhydryl group.

5 fluorescent probe (monobromobimane) to label sulfhydryl groups.

6 following hydrogen-bond classes for cysteine sulfhydryl groups.

7 maleimide, reagents that can modify cysteine sulfhydryl groups.

8 concentration-dependent oxidation of protein sulfhydryl groups.

9 rapped intermediates via cyanylation of free sulfhydryl groups.

10 two-cysteine residues and contained two free sulfhydryl groups.

11 of Ag(+) and/or Cd(+2) with the substituted sulfhydryl groups.

12 achieved by a coupled reaction with protein sulfhydryl groups.

13 decrease in the availability of lipoic acid sulfhydryl groups.

14 compounds are nonpeptidic and do not contain sulfhydryl groups.

15 thyl methanethiosulfonate to block unreacted sulfhydryl groups.

16 ram, is a selective carbamoylating agent for sulfhydryl groups.

17 dified by a mechanism altering the status of sulfhydryl groups.

18 e reducing agent dithiothreitol restored the sulfhydryl groups.

19 ated As was present as trivalent As bound by sulfhydryl groups.

20 isulfide bridges with cysteine or methionine sulfhydryl groups.

21 x1, which was mainly fully reduced with five sulfhydryl groups.

22 fluorescence detection of peptides with free sulfhydryl groups.

23 proteins and other ligands to silica through sulfhydryl groups.

24 be in the protection of superoxide-sensitive sulfhydryl groups.

25 the dynamic chemistry played by wine protein sulfhydryl groups.

26 e (GSH) is the major source of intracellular sulfhydryl groups.

27 oes not occur through direct inactivation of sulfhydryl groups.

28 sured were the levels of prolidase and total sulfhydryl groups.

29 of salvinorin A and an analogue with a free sulfhydryl group, 2-thiosalvinorin B.

30 We found that agents that disrupt free sulfhydryl groups abolish AP-1 binding activity in nucle

31 res higher than 200 MPa, a decrease in total sulfhydryl groups and an increase in surface hydrophobic

32 ary lipid oxidation products and the loss in sulfhydryl groups and Ca(2+)-ATPase activity.

33 ified the GR protein by decreasing available sulfhydryl groups and decreasing nuclear GR expression a

34 It triggers a rapid covalent destruction of sulfhydryl groups and disulfide bonds via the mechanism

35 Dithiothreitol treatment restored the sulfhydryl groups and enzyme activity.

36 Washed protein pellets were assayed for free sulfhydryl groups and for mixed protein disulfides with

37 Nonprotein sulfhydryl groups and glutathione reductase were not aff

38 Decay involves the increase in exposure of sulfhydryl groups and hydrophobic areas.

39 tein carbonyl content, oxidised amino acids, sulfhydryl groups and immuno-blotting against carbonyl g

40 nate (MTSES), used to irreversibly bind free sulfhydryl groups and inactivate the redox site, abolish

41 ocess, a recombinant E-cadherin lacking free sulfhydryl groups and its mutants with novel cysteines w

42 The level of accessible free sulfhydryl groups and the surface hydrophobicity of unfo

43 arsenite; and d) As has a high affinity for sulfhydryl groups and therefore binds to GSH and Cys.

44 peroxide, and phenylhydrazine) to cross-link sulfhydryl groups and with one fixative reagent (glutara

45 ), has no other active redox moieties (e.g., sulfhydryl groups) and can exist in three different oxid

46 ity on SDS-polyacrylamide gels, lack of free sulfhydryl groups, and atypical circular dichroism spect

47 ivation involves the oxidation of ER-iPLA(2) sulfhydryl groups, and that ER-iPLA(2) inhibition increa

48 and Msh2-Msh6 interactions involve cysteine sulfhydryl groups, and the high Cd(2+):Msh2-Msh6 ratio i

49 As isolated, this sulfhydryl group appears to be in a disulfide linkage wi

50 ion was accompanied by the appearance of new sulfhydryl groups as determined with 5, 5'-dithiobis(2-n

51 m of alpha-lipoic acid), which contains free sulfhydryl groups as NAC does, decreased Ang II receptor

52 ducted to determine the contribution of free sulfhydryl groups as well as the disulfide bonds in alle

53 Hyperreactive sulfhydryl groups associated with the Ca(2+) release pro

54 disulfide trapping reflects the proximity of sulfhydryl groups, assuming similar accessibility and fl

55 d Hb As, Hb A-IAA and Hb A-NEM, in which the sulfhydryl group at beta93Cys is modified by sulfhydryl

56 jor site of HSA adduction is the single free sulfhydryl group at Cys34, we used thiol-affinity resins

57 182 of cyt b or 46 of the Fe-S protein or a sulfhydryl group at position 46 of cyt c1.

58 of single stranded DNA (ssDNA) probe through sulfhydryl group at the 5' phosphate end.

59 signal, suggesting the involvement of a free sulfhydryl group at this location in receptor-ligand int

60 The sulfhydryl groups at the end of the molecular wire form

61 ate to cysteine, most likely indicating that sulfhydryl groups at these positions are appropriately s

62 Of particular interest is Cys(95), whose sulfhydryl group becomes exposed to modification in the

63 e nitrosonium ion, transnitrosylating a free sulfhydryl group belonging to a cysteine residue tentati

64 ctional preinitiation complexes by modifying sulfhydryl groups but did not have any significant effec

65 gest that post-translational modification of sulfhydryl groups by a nitrogen monoxide (likely to be N

66 roperties except for their ability to modify sulfhydryl groups by oxidation, reduction, or alkylation

67 double mutant proves that mere loss of free sulfhydryl groups by oxidative regulation cannot account

68 ns that favored closure, indicating that the sulfhydryl groups come close enough to each other or to

69 subpopulation of alphaIIbbeta3 contains free sulfhydryl groups, consistent with the possibility that

70 ionized selenol group in place of a cysteine sulfhydryl group could render this mammalian selenocyste

71 ition of NF-kappaB activity, suggesting that sulfhydryl groups critical for NF-kappaB activation were

72 Two sulfhydryl groups (Cys284 and Cys373) became inaccessibl

73 nd B2 remained unchanged, while free exposed sulfhydryl groups decreased.

74 pha-lipoic acid, which does not contain free sulfhydryl groups, did not.

75 Levels of sulfhydryl groups, dominated mainly by the high levels o

76 sulfur into homocysteine, which serves as a sulfhydryl group donor in the synthesis of cysteine by r

77 ealed that this mutation made the regulatory sulfhydryl group energetically much more difficult to re

78 ogical effects through reaction with exposed sulfhydryl groups, especially pairs of adjacent thiols.

79 These results indicate that the introduced sulfhydryl groups face the conduction pore and suggest t

80 o-ACP, forming the ACP analog that lacks the sulfhydryl group for the attachment of acyl chains for f

81 ifferent active sites and provide a terminal sulfhydryl group for the attachment of acyl groups throu

82 This sulfhydryl group forms a charge relay system with Asp-26

83 ppears to be in a disulfide linkage with the sulfhydryl group from the phosphopantetheine moiety of t

84 creasing pH and indicated that the pK of the sulfhydryl group had a pK value of 7.3.

85 ugh the reversible oxidation of key cysteine sulfhydryl groups has been demonstrated to occur in vitr

86 Nitrosylation of protein sulfhydryl groups has been shown to confer nitric oxide

87 ide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activit

88 Fab fragment, Fab fragment containing a free sulfhydryl group (i.e., Fab-SH), and whole antibody PZs

89 e protein, thioredoxin, between two cysteine sulfhydryl groups (i.e., staple), followed by photochemi

90 as the assay of reactivity of the introduced sulfhydryl group identify candidate positions that are i

91 tain a more stable form for oral dosing, the sulfhydryl group in conjugate 1 was converted into a fun

92 -linker that specifically cross-links a free sulfhydryl group in one protein (DrrB) to an amine in an

93 vity revealed that the functionally critical sulfhydryl group in the flippase protein is buried in a

94 le hydrogen-bonded states of buried cysteine sulfhydryl groups in a native protein.

95 The presence of free sulfhydryl groups in five recombinant monoclonal antibod

96 duction-oxidation modifications of cysteinyl sulfhydryl groups in mature ADAM17 may serve as a mechan

97 ct of CAPE, it suggests the role of critical sulfhydryl groups in NF-kappa B activation.

98 ent a method for identifying spatially close sulfhydryl groups in proteins employing chemical cross-l

99 can be used to quantify reactions targeting sulfhydryl groups in proteins.

100 the redox state of the insulin receptor (IR) sulfhydryl groups in response to reduced glutathione (GS

101 We proved the absence of accessible sulfhydryl groups in the oxidized product using liquid c

102 This procedure permits reaction of sulfhydryl groups in the periplasmic space with MPB, but

103 The amount of titrated sulfhydryl groups in the protein concentration range inv

104 GSH maintains protein sulfhydryl groups in the reduced form and has been impli

105 the reductase activity is unique in that no sulfhydryl groups in the YfcG protein are covalently inv

106 enzymes that generate a product with a free sulfhydryl group, including histone acetyltransferases,

107 ion, but without increased reactivity of its sulfhydryl groups, indicating that active-site alteratio

108 nges, specific for thiamine and sensitive to sulfhydryl group inhibition.

109 Na+ -H+ exchange inhibitor amiloride and the sulfhydryl group inhibitor p-chloromercuriphenylsulphona

110 nism of inhibition with N-ethyl maleimide, a sulfhydryl group inhibitor, indicates that this inhibiti

111 ifunctional protein cross-linker targeted to sulfhydryl groups, introduces intra-tetrameric cross-lin

112 ine-persulfide (Cys-SSH) is a cysteine whose sulfhydryl group is covalently bound to sulfur (sulfane

113 arine by reducing agents suggests a critical sulfhydryl group is involved in NF-kappaB activation.

114 ing properties, indicating that the reactive sulfhydryl group is located within a transmembrane domai

115 lation by sulfhydryl reagents, this reactive sulfhydryl group is on Cys-199 of the receptor.

116 sted that the presence of low levels of free sulfhydryl groups is likely a common feature of recombin

117 This further ensured that any sulfhydryl groups liberated during the digestion would b

118 ently interact with a class of hyperreactive sulfhydryl groups localized on ryanodine-sensitive Ca2+

119 yltriglycine with S-acetyl protection of the sulfhydryl group may be used to conjugate MAG3 to primar

120 Because spontaneous modification of sulfhydryl groups may also occur in vivo, tumor vaccines

121 er yellow iodoacetamide (LYI), suggests that sulfhydryl groups might play a role in the catalytic mec

122 s a T-rich probe DNA at one vertex and three sulfhydryl groups modified with 10nm Au NPs at the other

123 e of the enzyme and that AN was sensitive to sulfhydryl-group-modifying reagents in general.

124 sence of 1 mM MgADP revealed nearly two free sulfhydryl groups/mol of enzyme.

125 Besides the sulfhydryl group of a cysteine, we make use of an azido

126 f the C-terminal amino acid is linked to the sulfhydryl group of a cysteine, which is always the fift

127 ences of chemically modifying the Cys beta93 sulfhydryl group of both the deoxy and CO-saturated deri

128 lfide exchange between the inhibitor and the sulfhydryl group of C159.

129 inhibitor of the enzyme, suggesting that the sulfhydryl group of CoA is important for the optimizatio

130 es and that oxidized Cys(48) reacts with the sulfhydryl group of Cys(152) to form an intramolecular d

131 ally replaced with serine suggested that the sulfhydryl group of Cys(48) is the site of oxidation by

132 luding His-146 and Glu-172 that activate the sulfhydryl group of Cys-145 to allow its nucleophilic at

133 minal carbon of the acetylene moiety and the sulfhydryl group of Cys-803 at the solvent interface.

134 The reactive sulfhydryl group of Cys106 projects toward position C-4a

135 e presence of monovalent copper ions and the sulfhydryl group of Cys466.

136 e a counter ion to promote ionization of the sulfhydryl group of GSH, thereby accounting for the obse

137 olution containing NEM which reacts with the sulfhydryl group of GSH, thus locking the active form in

138 process, involves reaction of the side chain sulfhydryl group of homocysteine with its activated carb

139 nent of complement (C3), we coupled the free sulfhydryl group of methylamine-inactivated C3 to a thio

140 Whereas replacement of the sulfhydryl group of tetrapeptide CVLS (I50 = 1 microM) b

141 g peroxyl radical may react further with the sulfhydryl group of the C419 residue to form the sulfiny

142 confirmed by examining the reactivity of the sulfhydryl group of the Cysbeta93 toward 4, 4'-dipyridyl

143 ules were immobilized through the side-chain sulfhydryl group of the cysteine residue on several supp

144 disulfide interchange between an engineered sulfhydryl group of the protein (Q258C mutation) and a d

145 ilic attack of either the amine group or the sulfhydryl group of the substrate on the internal aldimi

146 te (APMA) work by directly reacting with the sulfhydryl group of this cysteine residue, resulting in

147 t-activated rhodopsin, the reactivity of the sulfhydryl groups of a constitutively active R175Q arres

148 The sulfhydryl groups of all mutants reacted rapidly with th

149 rategy was demonstrated by cross-linking the sulfhydryl groups of Cys-18 and Cys-78 in gamma-crystall

150 Reaction of MBB with the sulfhydryl groups of free cysteines leads to formation o

151 nge between thioredoxin f and the regulatory sulfhydryl groups of fructose-1,6-bisphosphatase, sedohe

152 g assay was designed to detect alkylation of sulfhydryl groups of human Keap1.

153 Arsenic binding by sulfhydryl groups of natural organic matter (NOM) was re

154 ecently, the complexation of trivalent As by sulfhydryl groups of NOM was proposed as the main mechan

155 Sulfhydryl groups of protein-cysteines constitute main t

156 Nitric oxide (NO) reacts with the sulfhydryl groups of proteins to form nitroso thiols.

157 be associated with its direct action on the sulfhydryl groups of the channel protein.

158 All three of the sulfhydryl groups of the mutant arrestin R175Q reacted r

159 The sulfhydryl groups of the three cysteines in bovine arres

160 redox midpoint potentials for the regulatory sulfhydryl groups of the various enzymes spanned a broad

161 The reactive sulfhydryl group on Cys beta93 in human adult hemoglobin

162 is-(2-nitrobenzoic acid), which modifies the sulfhydryl group on cysteines, caused irreversible disso

163 g that all three compounds acted at the same sulfhydryl group on the alternative oxidase protein.

164 hilic additions with reagents containing two sulfhydryl groups on adjacent carbon atoms to form a cyc

165 hese findings, we conclude that oxidation of sulfhydryl groups on intracellular targets results in in

166 modification of 1 or more of the 27 cysteine sulfhydryl groups on Keap1 (a protein that sequesters Nr

167 The addition of acrylamide to sulfhydryl groups on proteins is modeled using glutathio

168 disulfide bonds between low m.w. thiols and sulfhydryl groups on specific cytosolic proteins (S-thio

169 tion resulted in cross-linking of the unique sulfhydryl groups on subunit B with other subunits in th

170 hat these methods had a high selectivity for sulfhydryl groups on this protein, which accounted for t

171 on in vitro and decreased the number of free sulfhydryl groups on tubulin cysteines.

172 reas the polar or chemical properties of the sulfhydryl group play only a minor role; 3) the function

173 cysteine, which adds to the cofactor via its sulfhydryl group, possibly forming a cyclic thiazolidine

174 in gold or self-assembled monolayer via the sulfhydryl groups present in the hinge region.

175 N-ethylmaleimide (NEM) was used to mask free sulfhydryl groups present in the mAb.

176 n of complexes involving mercury species and sulfhydryl groups present in tissues and/or loss of wate

177 ivity, and this activity could be blocked by sulfhydryl group-reactive inhibitors, N-ethylmaleimide a

178 t protein damage, characterised by the total sulfhydryl group reduction.

179 of actin, IAM was used to block any residual sulfhydryl groups remaining on the protein so that they

180 pproach, a cemadotin derivative containing a sulfhydryl group results in a mixed disulfide linkage.

181 Two GSH analogues that lack a sulfhydryl group, S-hexylglutathione (SHG) and oxidized

182 se enzymes and as ionization of the Cys(184) sulfhydryl group seems required for sortase activity, we

183 strategy presents a rapid method for mapping sulfhydryl groups separated by a distance of approximate

184 Reversibly oxidized cysteine sulfhydryl groups serve as redox sensors or targets of r

185 is simulation that the distances between the sulfhydryl group (SG) of Cys81 to the C6 of Cyt18 (SG-C6

186 Binding of arsenite (As(III)) to sulfhydryl groups (Sorg(-II)) plays a key role in As det

187 lkylation of mature sublancin showed no free sulfhydryl groups, suggesting that one sulfydryl had for

188 Rabbit Fab'-SH fragments contain a free sulfhydryl group that forms a thiolate bond with a gold

189 This protein has a reactive sulfhydryl group that is essential for activity.

190 sidue disulfide loop by selectively labeling sulfhydryl groups that were liberated only after chemica

191 only in those fragments that contained free sulfhydryl groups: the light chain (Cys-1960, Cys-2113),

192 Methyl acrylate reacted instantly with the sulfhydryl group to form a derivative that was stable in

193 to the terminal carbon of homoserine via its sulfhydryl group to form cystathionine, which is cleaved

194 oyl cofactor, which is the attachment of two sulfhydryl groups to C6 and C8 of a pendant octanoyl cha

195 nd the use of a molecular wire terminated in sulfhydryl groups to connect the two modules.

196 the selective oxidation of protein cysteine sulfhydryl groups to disulfide bonds we examined the spe

197 The oxidation of sulfhydryl groups to disulfides as another possible sour

198 chicken egg white catalyzes the oxidation of sulfhydryl groups to disulfides with reduction of oxygen

199 chicken egg white catalyzes the oxidation of sulfhydryl groups to disulfides with the reduction of ox

200 de of cysteine residues after cyanylation of sulfhydryl groups to form an amino-terminal peptide and

201 trosylhemoglobin, NO can react with cysteine sulfhydryl groups to form S-nitrosocysteine or cysteine

202 by analyzing accessibility of the introduced sulfhydryl groups to thiophilic Cd(2+).

203 ioredoxin and inhibits its ability to reduce sulfhydryl groups via NADPH oxidation.

204 lycol-conjugated maleimide of 5 kDa for each sulfhydryl group, we find that PDI reduction and oxidati

205 ng advantage of the reactivity of cysteine's sulfhydryl group, we modified these mutants with chemica

206 The locations of the free sulfhydryl groups were determined using mass spectrometr

207 plasmin activity, prolidase level, and total sulfhydryl groups were evaluated.

208 Free sulfhydryl groups were first modified using 5-idoacetami

209 tially reduced sillucin species; the nascent sulfhydryl groups were immediately cyanylated by 1-cyano

210 This protection does not extend to the mAAT sulfhydryl groups which in the GroEL-mAAT complex have s

211 Modification of the introduced sulfhydryl group with membrane-impermeant methanethiosul

212 Blocking the beta-93 sulfhydryl group with N-ethylmaleimide was found to elim

213 reitol and was blocked by modification of Ng sulfhydryl groups with 4-vinylpyridine.

214 Titration of protein sulfhydryl groups with 5,5'-dithiobis(2-nitrobenzoate) (

215 and enhanced reactivity of one of arrestin's sulfhydryl groups with inhibition of the reactivity of a

216 s with dithiothreitol and alkylated the free sulfhydryl groups with iodoacetamide.

217 Redox-dependent modifications of sulfhydryl groups within the two Cys4 zinc fingers of th