ライフサイエンスコーパス: sulfotyrosine

1 ucing specific intermediates with one or two sulfotyrosines.

2 ecific intermediates with one, two, or three sulfotyrosines.

3 ligand-receptor interactions and identifies sulfotyrosine 174 as the critical C3a docking site.

4 t on the CXCL12 surface normally occupied by sulfotyrosine 21 (sY21), and five were selected for expe

5 tide stabilizes dimeric SDF-1alpha, and that sulfotyrosine 21 binds a specific site on the chemokine

6 xtreme N-terminal region of CXCR4, including sulfotyrosine 21, make specific contacts with the chemok

7 s containing unmodified tyrosine (160-fold), sulfotyrosine (3600-fold), phosphotyrosine (>8000-fold),

8 Although the vGPCR variant lacking sulfotyrosines activated downstream signaling pathways,

9 idues in V(H) CDR3 were randomized, contains sulfotyrosine and binds gp120 more effectively than a si

10 gonal aminoacyl-tRNA synthetase specific for sulfotyrosine and its cognate orthogonal tRNA that recog

11 Sulfotyrosine and other negatively charged residues in t

12 d with wild-type sperm treated with the anti-sulfotyrosine antibody PSG2.

13 e, along with a simple chemical synthesis of sulfotyrosine, are outlined in this protocol.

14 further demonstrated that the binding of the sulfotyrosine-binding pocket by CCR5mim2-Ig was sufficie

15 on and illustrate the utility of binding the sulfotyrosine-binding pockets of gp120.

16 eptor associated with Nt peptides containing sulfotyrosines but not with peptides containing sulfotyr

17 f the complex between the CXCL12 dimer and a sulfotyrosine-containing CXCR4 fragment enabled high-thr

18 phosphorylation and vGPCR tumorigenesis in a sulfotyrosine-dependent manner.

19 onserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the

20 Consistent with a functional role of the V2 sulfotyrosines, enhancement of tyrosine sulfation decrea

21 genetic code that co-translationally inserts sulfotyrosine in response to the amber nonsense codon, T

22 In addition to sulfotyrosines in positions 10 and 14, negatively charge

23 responding to CCR5 Nt residues 2 to 18, with sulfotyrosines in positions 10 and 14.

24 fotyrosines but not with peptides containing sulfotyrosines in scrambled Nt sequences.

25 Acidic residues and sulfotyrosines in the amino-terminal domain (Nt) of CCR5

26 Finally, only peptides containing sulfotyrosines inhibited the entry of an R5 isolate.

27 ere we report the selective incorporation of sulfotyrosine into proteins in bacteria by genetically e

28 The presence of sulfotyrosine is indicated by the detection of free tyro

29 er conditions in which the sulfuryl group of sulfotyrosine is labile.

30 tivation triggered by chemokine agonists via sulfotyrosines is necessary for vGPCR tumorigenesis, the

31 These results identify the sulfotyrosine-mediated V2-V3 interaction as a critical c

32 We have developed a novel anti-sulfotyrosine monoclonal antibody (called PSG2) that bin

33 ing affinity chromatography on PSG2, an anti-sulfotyrosine monoclonal antibody, followed by mass spec

34 Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar struct

35 ms the fluorosulfated tyrosine peptides into sulfotyrosine peptides in high yield.

36 y of CXCR4 1-38 increases with the number of sulfotyrosines present, which suggests a potential physi

37 Our results suggest that sulfotyrosine recognition sites can be targeted for the

38 ing two other binding pockets that recognize sulfotyrosine residues (sY12 and sY21) of CXCR4, includi

39 h high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independ

40 uch that they would be able to interact with sulfotyrosine residues on C4.

41 he anion-binding exosites of the enzymes via sulfotyrosine residues.

42 acts with a peptide from C4 containing three sulfotyrosine residues.

43 zymes, leading to a final product with three sulfotyrosine residues.

44 th therapeutic potential contain one or more sulfotyrosine residues.

45 enzymes leading to a final product with four sulfotyrosine residues.

46 e autoantibodies, we incorporated pNO(2)Phe, sulfotyrosine (SO(3)Tyr), and 3-nitrotyrosine (3NO(2)Tyr

47 Anti-sulfotyrosine staining of sperm showed localization simi

48 g interactions in phosphotyrosine (pTyr) and sulfotyrosine (sTyr) residues.

49 ovides interaction sites with the hormone: a sulfotyrosine (sTyr) site in the hinge region consistent

50 We found that sulfotyrosine (Tys) 278 enhances the interaction mainly

51 oacyl-tRNA synthetase/tRNA pair specific for sulfotyrosine, we were able to determine the contributio