ライフサイエンスコーパス: sumoylate

1 pecificity factor and poly(A) polymerase are sumoylated.

2 ucleus, but non-nuclear proteins can also be SUMOylated.

3 Here we show that the RecQ homolog, Rqh1, is sumoylated.

4 pon loading that enhances its capacity to be sumoylated.

5 e investigated the ability of ataxin-1 to be SUMOylated.

6 UMO E2 conjugating enzyme Ubc9 and is itself sumoylated.

7 he sumoylation cascade were found to be auto-sumoylated.

8 eins, and nuclear pore complex proteins were sumoylated.

9 w that Lys(684) and Lys(897) of NFAT1 can be sumoylated.

10 AR lacking the two lysine residues that are SUMOylated.

11 several cellular proteins, some of which are sumoylated.

12 wever, unlike wild-type cells, mut5 fails to SUMOylate a large set of proteins in response to multipl

13 dentified K68 and K284 as critical sites for SUMOylating actin.

14 re, slx5 cells accumulate phosphorylated and sumoylated adducts of Siz1 in vivo.

15 at multiple Tup1-interacting proteins become sumoylated after stress.

16 , PHD, Bromo, and HMG domains and are highly sumoylated, all characteristics suggestive of a role in

17 rked increase in PIAS2 expression along with SUMOylated alpha-synuclein in PD brains, providing a cau

18 tide-binding protein Rhes, which is known to sumoylate and disaggregate mtHtt.

19 We show that all four core histones are sumoylated and identify specific sites of sumoylation in

20 ncreased nuclear abundance, can no longer be SUMOylated and is no longer effective in inhibiting LeEi

21 Endogenous Sp3 is sumoylated and localized to the nuclear periphery and in

22 The sumoylated and nonsumoylated forms of Z were found to ha

23 the corresponding nonactivatable MEK1 is not SUMOylated and nuclear.

24 We found that FOG1 is SUMOylated and phosphorylated in erythroid cells in a di

25 stress through regulation of the turnover of sumoylated and phosphorylated proteins.

26 rgeted Ub ligase display increased levels of sumoylated and polysumoylated proteins, and they are inv

27 ias (WM) using protein macroarrays that were sumoylated and screened for reactivity with paraproteins

28 In this study, we demonstrate that CtBP1 was SUMOylated and that its SUMOylation profoundly affected

29 gether, our findings indicate that merlin is sumoylated and that this post-translational modification

30 sidues abolished the ability of S100A4 to be sumoylated and to translocate into the nucleus.

31 Our analysis revealed that H2AX is both sumoylated and ubiquitylated.

32 rement for ICP0-mediated degradation of both sumoylated and unmodified promyelocytic leukemia (PML) a

33 In Saccharomyces cerevisiae, both sumoylating and desumoylating activities are essential f

34 LIN-11 is sumoylated, and its sumoylation is required for its acti

35 cytic leukemia (PML) nuclear bodies, becomes SUMOylated, and recruits corepressor RIP140 to act as a

36 Three DCC subunits are SUMOylated, and SUMO depletion preferentially reduces th

37 la melanogaster, Mod(mdg4)2.2 and CP190, are sumoylated, and that SUMO is associated with a subset of

38 of several genes implicated in clefting are sumoylated, and the Sumo1 hypomorphic allele interacts g

39 However, NS1's ability to be SUMOylated appears to affect virus multiplication, as in

40 that expressed a RPA70 mutant that cannot be SUMOylated are defective in HR and have a marked increas

41 Conversely, constitutively SUMOylated ARL-13 fully rescues all ciliary defects of a

42 only a small fraction of a target protein is sumoylated at a given time.

43 Here we show that KLF4 is both SUMOylated at a single lysine residue and physically int

44 We show that CtBP is sumoylated at a single lysine.

45 We determined that p68 and p72 are indeed sumoylated at a single, homologous site.

46 s, only a small fraction of the substrate is sumoylated at any given time.

47 We found that SF-1 is selectively SUMOylated at K194 in Y1 adrenocarcinoma cells and that

48 nerate H4 homogenously and site-specifically sumoylated at Lys-12 (suH4ss).

49 ation results clearly demonstrate that PIP5K SUMOylated at Lys-490 interacts with components of the c

50 We show that lamin A is sumoylated at lysine 201 and that two lamin A mutants as

51 Wor1 is found to be SUMOylated at lysine 385, and loss of this mark by point

52 Finally, we identified that IRF7 is sumoylated at lysine 452.

53 analysis, we demonstrate that TopoIIalpha is SUMOylated at lysine 660 (Lys660), a residue located in

54 Here we show that mouse KLF5 is SUMOylated at lysine residues 151 and 202.

55 nscriptional repressor form of MEF2A that is sumoylated at lysine-403 promoted dendritic claw differe

56 TRalpha was sumoylated at lysines 283 and 389, and TRbeta at lysines

57 Here we show that SERCA2a is SUMOylated at lysines 480 and 585 and that this SUMOylat

58 Here, we provide evidence that K-bZIP is sumoylated at the lysine 158 residue and associates with

59 KLF2 was sumoylated at two conserved neighboring motifs, but subs

60 Here we show that the corepressor Tup1 is sumoylated, at two specific lysines, under various stres

61 the nucleolar-remodeling complex (NoRC), and SUMOylated BEND3 stabilizes NoRC component TTF-1-interac

62 SUMOylated beta-catenin accumulates at the chromatin and

63 In vivo, SUMOylated beta-catenin and Axin1 are both SENP7S-substr

64 Indeed, Ran is sumoylated both by a reconstituted and the endogenous Ra

65 e SUMO E2 enzyme ubc9 and can be extensively sumoylated both in vitro and in vivo.

66 Here we report that YY1 protein can be sumoylated both in vivo and in vitro.

67 r proteins are likely to be ubiquitinated or sumoylated but not acetylated.

68 epression of the ferritin H ARE; 2) ATF1 was sumoylated, but PIAS3, a SUMO E3 ligase, did not appear

69 Top1 is sumoylated, but Top1 does not appear to be the SUMO subs

70 ZNF76 is sumoylated by PIAS1 at lysine 411, which is in the minim

71 Here, we demonstrated that GATA4 is sumoylated by small ubiquitin-like modifier-1 (SUMO-1),

72 kinase catalytic subunit and, subsequently, SUMOylated by SUMO E3 ligases protein inhibitors of acti

73 for the sumoylation of Z, and that Z can be sumoylated by SUMO isoforms 1, 2, and 3.

74 , our results reveal that p53 and pRB can be sumoylated by SUMO-2/3 in vivo, and such modification of

75 econd, in vitro-synthesized p32 Pax-6 can be sumoylated by SUMO1, and the sumoylated p32 Pax-6 then c

76 that the K391 and K436 residues of TOP1 are SUMOylated by the PIAS1-SRSF1 E3 ligase complex in the c

77 Arabidopsis RanGAP1 (AtRanGAP1) lacks the SUMOylated C-terminal domain of vertebrate RanGAP, but c

78 and kinetochores requires interaction of its sumoylated C-terminal domain with the nucleoporin Nup358

79 suggest a model in which Flp that cannot be sumoylated causes DNA damage, whose repair via HRR produ

80 Sumoylated CBS is present in the nucleus where it is ass

81 The global profile of SUMOylated cell proteins was also suppressed by BGLF4 bu

82 ears to be ubiquitin-mediated degradation of sumoylated cellular proteins.

83 ified promyelocytic leukemia (PML) and other sumoylated cellular proteins.

84 at the CDC48A(NPL4) complex actively removes sumoylated CenH3 from centromeres and disrupts centromer

85 alpha-Synuclein disease mutants are more SUMOylated compared with the wild-type protein, and this

86 SUMOylated cSHMT was detected in extracts from S phase M

87 The sumoylated CTE fragment (CTE-SUMO-1) accumulates in the

88 o substantially increases the basal level of SUMOylated Daxx observed in cells.

89 Thus, while pp71 enhances the basal level of SUMOylated Daxx, the role that this modification plays i

90 SUMOylated DCC subunits are enriched at recruitment site

91 gated DELLAs leads to an accumulation of non-SUMOylated DELLAs, resulting in beneficial growth restra

92 onstrated that promoter-bound factors become sumoylated during activation of inducible genes in yeast

93 showed that the yeast activator Gcn4 becomes sumoylated during activation, facilitating its eventual

94 We found that Mre11 and Nbs1 are sumoylated during Ad5 infection and that the E4-ORF3 pro

95 ular that endogenous SEPT7 is constitutively SUMOylated during the cell cycle.

96 s released by murine cerebellar neurons as a sumoylated dynamin-containing protein upon L1 stimulatio

97 tates the loss of the repressive HDAC-2 from sumoylated Elk-1, a key event in the activation of Elk-1

98 This review will focus on the de-SUMOylating enzymes with special attention to their biol

99 y was not necessary for the SUMOylation, the SUMOylated ErbB4 ICD was tyrosine phosphorylated to a hi

100 of a K-bZIPK158R mutant, which was no longer sumoylated, exhibited the reduced transcriptional repres

101 d that SIRT1 is expressed predominantly as a sumoylated form in cardiomyocyte nuclei.

102 regulation correlates with accumulation of a SUMOylated form of GATA1.

103 ity to small Rho GTPase compared with the un-SUMOylated form of RhoGDI.

104 causes increased steady-state levels of the sumoylated forms of a number of proteins and results in

105 Moreover, SUMOylated forms of mitochondrial proteins particularly

106 g cellular sumoylated species in general and sumoylated forms of PML other than those of PML isoform

107 e recruitment Sp100A(K297R), which cannot be sumoylated, further suggests that sumoylation plays an i

108 Furthermore, we show that clearance of sumoylated Gcn4 requires the protein kinase and Mediator

109 anese patients specifically reacted with the sumoylated heat-shock protein 90 beta isoform-alpha (HSP

110 SUMOylated HP1alpha is enriched at E2F-responsive and me

111 SUMO1-specific paraproteins, suggesting that sumoylated HSP90 promotes pathogenesis of these diseases

112 g deficit was extended to all sites when the sumoylated human mutant (R92Q) protein, which exhibits l

113 The SUMOylated IkappaBalpha form is resistant to signal-indu

114 dent transcriptional repression function for SUMOylated IkappaBalpha.

115 r et al. show that genotoxic stress requires SUMOylated IKKvarepsilon to regulate NF-kappaB transcrip

116 re, we show that ORF29p is ubiquitinated and sumoylated in 293T cells and subsequently degraded from

117 analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 un

118 The ID complex is SUMOylated in a manner that depends on the ATR kinase, t

119 ear the scaffolding domain and that Cav-3 is SUMOylated in a manner that is enhanced by the SUMO E3 l

120 MO-conjugating enzyme (E2), UBC9, and can be sumoylated in an E1 (SUMO-activating enzyme)- and E2-dep

121 moylation, we hypothesized that keratins are sumoylated in an injury-dependent manner and that kerati

122 SOX9 was sumoylated in cotransfection experiments with COS-7 cell

123 DK6 SUMOylation during mitosis; CDK6 remains SUMOylated in G1 phase and drives the cell cycle through

124 tion under resting conditions and is rapidly SUMOylated in response to a kainate but not an N-methyl-

125 tiple proteins of a complex are collectively SUMOylated in response to a specific stimulus, leading t

126 that K102 is the sole CRABP-II residue to be SUMOylated in response to RA.

127 teins FANCI and FANCD2 (the ID complex), are SUMOylated in response to replication fork stalling.

128 We demonstrate that FoxM1b can be SUMOylated in vitro and in vivo, preferentially by SUMO-

129 SOX9 was also sumoylated in vitro by PIAS proteins in the presence of

130 e E2 SUMO-conjugating enzyme Ubc9 and can be SUMOylated in vitro using purified recombinant component

131 e pancreatic isoform of human glucokinase is SUMOylated in vitro, using recombinant enzymes, and in i

132 18/K19, epidermal keratins, and vimentin are sumoylated in vitro.

133 Using a reconstitution assay, Kar9p was sumoylated in vitro.

134 Rad1's nuclease and Rad10-binding domains is sumoylated in vivo and in vitro.

135 Here, we demonstrate that Mot1 is SUMOylated in vivo and that disrupting the Slx5-Slx8 pat

136 We found that alpha-synuclein is sumoylated in vivo at the same sites in yeast as in huma

137 ent for MDM2 binding (p53(14Q19S)) is poorly sumoylated in vivo compared to wild-type p53.

138 in vitro, only a small substrate fraction is SUMOylated in vivo, and identification tools for nativel

139 TbAUK1 is SUMOylated in vivo, and mutation of the SUMO-conjugation

140 served Nedd4 family of ubiquitin ligases, is SUMOylated in vivo.

141 s with components of the SUMO pathway and is SUMOylated in vivo.

142 fluenza virus and one of the most abundantly SUMOylated influenza virus proteins.

143 egulatory factor 5 (IRF5) as a chaperone for sumoylated IRAK1 nuclear translocation.

144 Our data show that endogenously sumoylated IRF7 is detected in latently infected EBV lym

145 on caused a sharp reduction in the amount of SUMOylated IRF8.

146 e components of a single protein complex are SUMOylated, is also conserved.

147 it Sp1 functions through several mechanisms: sumoylating it at K683 to attenuate DNA binding, and at

148 Sumoylated KAP1 stimulates the histone methyltransferase

149 showed that the SIM peptide interacted with SUMOylated Ku70 after radiation.

150 ked by silencing RNA directed at the primary SUMOylating ligase, Ubc9.

151 SUMOylated LXRs block NCoR turnover by binding to a cons

152 We identify over a thousand sumoylated lysines in a total of 468 proteins and quanti

153 Although sumoylated MBD1 binds to methylated DNA, it does not inc

154 Collectively, these findings define sumoylated MEF2A and Syt1 as components of a novel cell-

155 sites, as a direct repressed target gene of sumoylated MEF2A in neurons, and demonstrate that repres

156 itic claw differentiation, and expression of sumoylated MEF2A reverses PIASx knockdown-induced loss o

157 its loss causes subunit-specific changes of sumoylated minichromosome maintenance (MCM) helicase in

158 SUMOylated misfolded proteins are then recognized and ub

159 that tumor necrosis factor-alpha (TNF-alpha) SUMOylated MK2 at lysine (K)-339 affected EC actin filam

160 wo target proteins and did not localize bulk sumoylated molecules to nucleoli.

161 Here we show that the Smc5/6 subunit Mms21 sumoylates multiple lysines of the cohesin subunit Scc1.

162 he E2 ligase Ubc9 to FOXM1 generated an auto-SUMOylating mutant (FOXM1-Ubc9).

163 (PR8) NS1 protein, demonstrating that NS1 is SUMOylated not only by SUMO1 but also by SUMO2/3 and map

164 Pias3 SUMOylates Nr2e3, converting it into a potent repressor

165 icase in addition to drastic accumulation of sumoylated nucleolar RENT and inner kinetochore complexe

166 rm of SUMO1) not only increased the level of sumoylated OCT4 (Su-OCT4), but also decreased the stabil

167 Here, we show that Ebp1 p42 isoform can be sumoylated on both K93 and K298 residues, which mediate

168 Here we show that B23 is sumoylated on both Lysine 230 and 263 residues, but the

169 Here, we show that Pin1 is SUMOylated on Lys6 in the WW domain and on Lys63 in the

170 trated that HIC1 can be either acetylated or SUMOylated on lysine 314.

171 Here we report that merlin can be sumoylated on Lysine residue (K76) in vitro and in vivo.

172 ind here that both WT1 isoforms are directly sumoylated on lysine residues 73 and 177.

173 re, we demonstrate that the SnRK1 complex is SUMOylated on multiple subunits and identify SIZ1 as the

174 w that the transcriptional activator Gcn4 is sumoylated on two specific lysine residues and in a mann

175 n motif (SIM)-dependent manner that requires SUMOylated or SUMOylation-competent PML.

176 We show that SoxE mutants that cannot be SUMOylated, or that mimic constitutive SUMOylation, are

177 egion previously shown to associate with and SUMOylate other transcription factors.

178 32 Pax-6 can be sumoylated by SUMO1, and the sumoylated p32 Pax-6 then can bind to the P3 sequence.

179 helicase (PICH) as an interaction partner of SUMOylated PARP1 in Xenopus egg extract.

180 em mass spectrometry analysis of mitotically SUMOylated PARP1, we identified a residue within the BRC

181 sequence of mitotic SUMOylation, we analyzed SUMOylated PARP1-specific binding proteins.

182 Some NB-associated sumoylated partners also become polyubiquitinated by RNF

183 unctions in unloading of both unmodified and SUMOylated PCNA during DNA replication, while the genome

184 tending D-loops over unextended D-loops when SUMOylated PCNA is present, compared to unmodified PCNA

185 Our results imply that sumoylated PCNA is the physiological ubiquitylation targ

186 We show that Srs2p physically interacts with sumoylated PCNA, which contributes to the recruitment of

187 g parallel quantitation of ubiquitylated and SUMOylated peptides.

188 the C terminus of phyB; the accumulation of SUMOylated phyB is enhanced by red light and displays a

189 how that OVERLY TOLERANT TO SALT 1 (OTS1) de-SUMOylates phyB in vitro, it interacts with phyB in vivo

190 Additionally, SUMOylated PIM1 showed enhanced protein kinase activity

191 s oncogenic signaling through its ability to sumoylate PML and the PML-RARA oncoprotein of acute prom

192 P0 also reduced ICP0-mediated degradation of sumoylated PML in a cooperative manner.

193 UMOylation and noncovalent binding of PML to SUMOylated PML through the SUMO binding motif constitute

194 A prominent ~50-kDa sumoylated protein accumulates in a Ulp1 CC mutant.

195 containing the Huntingtin (HTT) protein and SUMOylated protein corresponds to disease manifestation.

196 esult in dramatic changes in the global host SUMOylated protein profile, but a robust colocalization

197 High molecular weight sumoylated protein species, including PSF, accumulate in

198 ation sites in proteins are hard to predict, SUMOylated protein states are transient in vivo and labi

199 esults suggest that SUMO1, or more likely, a sumoylated protein, acts as an allosteric regulator of D

200 SUMOylated proteins accumulate in rfp1rfp2 double-null c

201 ion for this inviability is that one or more sumoylated proteins accumulate to toxic levels in sgs1De

202 propose that STUbLs selectively ubiquitinate sumoylated proteins and proteins that contain SUMO-like

203 cleation event for subsequent recruitment of SUMOylated proteins and/or proteins containing SUMO bind

204 nent Red1) and ubiquitin-mediated removal of SUMOylated proteins are also required.

205 d proteins is hampered by the fact that many sumoylated proteins are present at a level below normal

206 vivo, and identification tools for natively SUMOylated proteins are rare.

207 argeted ubiquitin ligases (STUbLs) recognize sumoylated proteins as substrates for ubiquitylation and

208 ion in yeast, we determined the occupancy of sumoylated proteins at a variety of genes by chromatin i

209 Surprisingly, we detected sumoylated proteins at all constitutively transcribed ge

210 s suggests that the CPEB1-ZZ domain recruits sumoylated proteins during assembly of the ribonucleopro

211 describe the identification of 21 candidate sumoylated proteins from whole-cell lysates of HEK-293 c

212 WaLP digestion of SUMOylated proteins generates peptides containing SUMO-r

213 s scarce information on chromatin binding of SUMOylated proteins in HS and the role of chromatin SUMO

214 that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress.

215 We have undertaken a global analysis of sumoylated proteins in Saccharomyces cerevisiae by tande

216 The presence of bona fide SUMOylated proteins in the mut5 mutant at 25 degrees C c

217 n of multiple additional subunits of SAGA as sumoylated proteins in vivo, these data suggest that Gcn

218 domain, lead to the accumulation of distinct sumoylated proteins in vivo.

219 However, the identification of endogenous sumoylated proteins is challenging because of the activi

220 The identification of SUMOylated proteins is difficult, because SUMOylation si

221 Unlike ubiquitin, detection of endogenous SUMOylated proteins is limited by the lack of naturally

222 In budding yeast, a subset of sumoylated proteins is targeted for ubiquitination by a

223 discovered a specific set of differentially sumoylated proteins mainly involved in transcription.

224 This discovery suggests that sumoylated proteins may be regulated by ubiquitylation i

225 ble for its cellular localization, and other sumoylated proteins may target WT1 to these nuclear stru

226 SENP1, in vitro, indicating the increase in SUMOylated proteins results primarily from inhibition of

227 Sumoylated proteins so obtained were affinity purified o

228 a ulp2-D623H displayed even higher levels of sumoylated proteins than the corresponding double mutant

229 loss of ULP2 suppresses the toxicity of the sumoylated proteins that accumulate in slx5Delta-slx8Del

230 ligase appears to be needed to ubiquitinate sumoylated proteins that arise in the absence of the Sgs

231 ancement enables determination of endogenous SUMOylated proteins under completely native conditions.

232 Substantial increases in SUMOylated proteins upon various stresses have also impl

233 The isolated sumoylated proteins were identified by sequence analysis

234 ng purification under denaturing conditions, SUMOylated proteins were identified by tandem mass spect

235 A large number of new sumoylated proteins were identified in untreated, hydrog

236 Ulp1 restricts Ulp1 activity toward certain sumoylated proteins while enabling the cleavage of other

237 of whole-cell extracts and nuclear localized SUMOylated proteins with in situ immunofluorescence.

238 Cells lacking Slx8-Rfp accumulate sumoylated proteins, display genomic instability, and ar

239 cannot be due simply to high levels of bulk sumoylated proteins.

240 nuclear domains with a high concentration of sumoylated proteins.

241 uantitative proteomics to identify nucleolar SUMOylated proteins.

242 receptors are activated indirectly by other SUMOylated proteins.

243 targets, contributing to a tight control of SUMOylated proteins.

244 g the specificity of STUbLs as regulators of sumoylated proteins.

245 which is a ubiquitin E3 ligase that targets sumoylated proteins.

246 and a concomitant reduction in the levels of SUMOylated proteins.

247 ns and its recognition of the SUMO moiety in sumoylated proteins.

248 from proteins that are known to bind SUMO or sumoylated proteins.

249 by promoting degradation of other pathogenic sumoylated proteins.

250 metalloprotease Wss1 clears chromatin-bound sumoylated proteins.

251 We propose that Mms21 sumoylates proteins involved in these diverse processes

252 Upon heat stress, AtBAG7 is sumoylated, proteolytically processed and translocated f

253 t the CAG tract suggests that Slx5/8 targets sumoylated Rad52 for degradation at the pore to facilita

254 SUMOylated RanGAP1 physically interacted with MYCBP2 and

255 or the interaction between RanBP2/Nup358 and sumoylated RanGAP1.

256 A protein mutated such that it cannot be SUMOylated remains localized in the cytoplasm rather tha

257 Our experiments show that SUMOylated Rsp5p has reduced ubiquitin ligase activity,

258 Thus, we demonstrate a novel mechanism for sumoylated S100A4 as a regulator of expression of the MM

259 cells expressing wt PR-B or phospho-mutant (sumoylated) S294A PR-B.

260 Associated Factor B (SAFB) protein, and the SUMOylated SAFB stimulated both the binding of RNA polym

261 rmation and coregulator recruitment of fully sumoylated SF-1 LBD protein was either unchanged or mode

262 teraction of the DEAD-box protein DP103 with sumoylated SF-1.

263 ase 2 (SENP2), significantly reduces nuclear sumoylated SIRT1 levels (P<0.05).

264 lx8, we assayed its Ub ligase activity using sumoylated Siz2 as an in vitro substrate.

265 STUbL recruitment to sumoylated/SLD proteins is mediated by tandem SUMO inter

266 at the SLX4 complex is a SUMO E3 ligase that SUMOylates SLX4 itself and the XPF subunit of the DNA re

267 Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIMDelta) or SUMO-dead alleles (

268 s (SIMs) on Sgs1 that specifically recognize SUMOylated Smc5/6.

269 e absolutely required for targeting cellular sumoylated species in general and sumoylated forms of PM

270 d ubiquitin ligases (STUbLs) can recognize a sumoylated substrate and promote its degradation via ubi

271 a cell-based screen that focused on the well-sumoylated substrate, human Liver Receptor Homolog-1 (hL

272 s a SUMO-dependent isopeptidase that acts on sumoylated substrates as they undergo proteasomal degrad

273 ified recombinant human PICH interacted with SUMOylated substrates, indicating that PICH directly int

274 ese modifications by selectively recognizing SUMOylated target proteins through SUMO-interacting moti

275 nonproteolytic, K63-linked ubiquitylation of SUMOylated target proteins.

276 n DNA damage Wss1/Cdc48/Doa1 is recruited to sumoylated targets and catalyzes SUMO chain extension th

277 es during interphase in human cells, but the SUMOylated targets on the chromatin remained unclear.

278 y promote ubiquitin-dependent degradation of SUMOylated targets.

279 r activity against a substantial fraction of sumoylated targets.

280 We purify SUMOylated TDP1 from mammalian cells and identify the SU

281 ingly, although it was much more efficiently sumoylated than either KLF2 or KLF4, KLF5 was inactive i

282 gating enzyme, physically interacts with and SUMOylates the C terminus of small GTPase ARL-13, the wo

283 lthough a small fraction of Z is known to be sumoylated, the effects of this posttranslational modifi

284 idopsis protoplasts, indicating that ABI5 is sumoylated through SIZ1 and that K391 is the principal s

285 vity further promotes TDP2 interactions with SUMOylated TOP2, regulating efficient TDP2 recruitment t

286 ust centromeric Haspin localization requires SUMOylated TOP2A CTD binding activity through SUMO-inter

287 We find that RanBP2 sumoylates Topo IIalpha in mitosis and that this modific

288 es identified a small fraction of putatively sumoylated topoisomerase I (TOP1) under basal conditions

289 Interestingly, PICH also bound to SUMOylated topoisomerase IIalpha (TopoIIalpha), a major

290 Remarkably, the sumoylated transcriptional repressor form of MEF2A drive

291 1 at the plasma membrane and generation of a sumoylated transmembrane 70-kDa fragment comprising the

292 S could promote the recruitment of PML-IV to SUMOylated TRF1 in TA(+) and ALT cells.

293 and that chromatin-bound Top2p can still be sumoylated, unlike many other SUMO substrates.

294 We found that the kinase PKC-theta was sumoylated upon costimulation with antigen or via the TC

295 We find that all subunits of cohesin become SUMOylated upon exposure to DNA damaging agents or prese

296 tive MEK1 is cytosolic and is constitutively SUMOylated, whereas the corresponding nonactivatable MEK

297 nally, we provide evidence that Cos2 is also sumoylated, which counteracts its inhibitory role on Smo

298 Elevated SENP7L renders HP1alpha hypo-SUMOylated, which relieves transcriptional repression of

299 we have found that the P protein of PIV5 was sumoylated with SUMO1 in both transfected and infected c

300 trate that RNF111 promoted ubiquitylation of SUMOylated XPC (xeroderma pigmentosum C) protein, a cent

301 otein phosphatase activity and ability to be SUMOylated, yet is independent of its lipid phosphatase