ライフサイエンスコーパス: synapsis

1 the nuclear envelope needed for pairing and synapsis.

2 nduce NE remodeling, chromosome pairing, and synapsis.

3 promote PC complex aggregation, pairing, and synapsis.

4 tial PC pairing to ensure correct homologous synapsis.

5 o define the first step in the initiation of synapsis.

6 ly, without any obvious defect in chromosome synapsis.

7 aded with Zip1p in a manner that may promote synapsis.

8 omeres is a continuation of the interstitial synapsis.

9 t of models for the role of PCs in promoting synapsis.

10 ngle RSS that are paired in trans to promote synapsis.

11 re essential for both homologous pairing and synapsis.

12 Zip2 and Zip4 are dispensable for chromosome synapsis.

13 defective DNA binding and no detectable DNA synapsis.

14 luding chromosome fragmentation, pairing and synapsis.

15 important and rate-limiting step to complete synapsis.

16 ermatocytes exhibit a failure in chromosomal synapsis.

17 ated mutants is slow relative to the rate of synapsis.

18 ve Y324F and R173K mutants are defective for synapsis.

19 omplexes remain stable but do not proceed to synapsis.

20 checkpoint pathway that monitors chromosome synapsis.

21 absence results in nonhomologous pairing and synapsis.

22 C) required for the regulation of chromosome synapsis.

23 ion, homologous recombination and chromosome synapsis.

24 -order nucleoprotein complexes necessary for synapsis.

25 in the presence or absence of GTP, prior to synapsis.

26 element ends in the absence of GTP prior to synapsis.

27 Y fuse through a dense plate without obvious synapsis.

28 mmobilized DNA fragments that cannot undergo synapsis.

29 -effect relationship between sumoylation and synapsis.

30 G proteins' ability to sense 12/23-regulated synapsis.

31 is due to a failure of homologous chromosome synapsis.

32 lure in the formation of AEs and chromosomal synapsis.

33 e (RSS)-conserved regions before nicking and synapsis.

34 X chromosome homolog pairing and subsequent synapsis.

35 zation fails to stabilize pairing or promote synapsis.

36 ate binding of Int to the P half site during synapsis.

37 some-specific defects in homolog pairing and synapsis.

38 hin transcribed S regions and mediates their synapsis.

39 osis and is probably required for homologous synapsis.

40 nd BUB-3 require full PC function to inhibit synapsis.

41 required during immunoglobulin switch region synapsis.

42 affecting homologous chromosome pairing and synapsis.

43 cytes that are severely defective in homolog synapsis.

44 2 supports homologous chromosome pairing and synapsis.

45 t has no known role following Rad51-mediated synapsis.

46 d is most likely important for initiation of synapsis.

47 ed for meiotic recombination and chromosomal synapsis.

48 ut that their primary function is to trigger synapsis.

49 on of recombination, chromosome pairing, and synapsis.

50 alpha) segments that should facilitate their synapsis.

51 cumulate unrepaired DNA and fail to complete synapsis.

52 rference-sensitive crossovers and chromosome synapsis.

53 tromeres is the initiating event for meiotic synapsis.

54 t period of postreplicative sister chromatid synapsis, a situation that is more reminiscent of eukary

55 dies lead us to propose that SYP-3 regulates synapsis along chromosomes, contributing to meiotic prog

56 ic processes that included recombination and synapsis, along with gene sets involved in RNA metabolis

57 tudies confirmed that polymerase-induced end synapsis also occurs in solution.

58 In Caenorhabditis elegans, synapsis and a checkpoint that monitors synapsis depend

59 s in severe defects in homologous chromosome synapsis and an early-stage failure in meiotic recombina

60 t this can be circumvented by preventing Y-Y synapsis and associated Y gene expression.

61 us chromosomes in atrad51c-1 fail to undergo synapsis and become severely fragmented.

62 ate domains of lambda-int to DNA facilitates synapsis and can specify the order of DNA strand cleavag

63 The involvement of some residues in both synapsis and catalysis suggests that they contribute to

64 d RAG-2 initiate V(D)J recombination through synapsis and cleavage of a 12/23 pair of V(D)J recombina

65 ns initiate V(D)J recombination by mediating synapsis and cleavage of two different antigen receptor

66 te that the mutants are defective in homolog synapsis and crossover formation, resulting in a reducti

67 iotic double-strand break repair, chromosome synapsis and crossover numbers.

68 Effects on male meiotic chromosome synapsis and derepression of transposable elements (TEs)

69 BPA exposure results in impaired chromosome synapsis and disruption of meiotic double-strand break r

70 op2 in mice eliminates homologous chromosome synapsis and disrupts double-strand break (DSB) repair t

71 prior to formation of recombinants (binding, synapsis and DNA cleavage).

72 ng a cell-free system that recapitulates end synapsis and DNA-PKcs autophosphorylation, we found a de

73 ced by full-length RAG1, and associated with synapsis and efficient RSS cleavage.

74 , or tension, between homologues to regulate synapsis and elicit a checkpoint response.

75 activity is required for homolog pairing and synapsis and for double-strand break formation, but how

76 11 promotes initiation and/or maintenance of synapsis and formation of crossovers, and may provide a

77 r DNA double-strand breaks (DSBs), involving synapsis and ligation of the broken strands.

78 1c results in complete sterility, incomplete synapsis and meiotic chromosome fragmentation, suggestin

79 vel protein that is required for chromosomal synapsis and meiotic recombination.

80 ption of the Raptor gene impairs chromosomal synapsis and prevents the efficient spreading of silenci

81 cytes displayed normal homologous chromosome synapsis and progression through meiosis.

82 nd BUB-3 are required to negatively regulate synapsis and promote the synapsis checkpoint response.

83 Meiotic synapsis and recombination between homologs permits the

84 t the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis.

85 rovide new insights on the interplay between synapsis and recombination in barley and highlight the n

86 ence of key features of meiosis I, including synapsis and recombination.

87 NA structures and Hop1 in meiotic chromosome synapsis and recombination.

88 ring meiosis, homologous chromosomes undergo synapsis and recombination.

89 ar hallmarks of meiotic chromosome cohesion, synapsis and recombination.

90 , meiotic chromosome condensation, cohesion, synapsis and recombination.

91 regions in switching B cells to promote DNA synapsis and recombination.

92 mechanism underlying its role in chromosome synapsis and recombination.

93 hromatin hub that supports V(alpha)-J(alpha) synapsis and recombination.

94 cations of our results for the mechanisms of synapsis and regulation in recombination by wild-type re

95 roles in determining the sequential order of synapsis and remodeling before end joining.

96 ister chromatid cohesion and enables correct synapsis and segregation of homologous chromosomes durin

97 SYN3 caused defects in homologous chromosome synapsis and synaptonemal complex (SC) formation during

98 complex, exhibits a high level of chromosome synapsis and that most DSBs in these spermatocytes are r

99 y, a male-specific organelle associated with synapsis and the formation of the XY body during meiosis

100 atocytes are defective in meiotic chromosome synapsis and undergo apoptosis during Prophase I.

101 XYY males exhibit Y-Y synapsis and Y chromosomal escape from MSCI without acco

102 However, these also harbor defects in synapsis and/or recombination and as such may activate a

103 naptonemal complex (SC) assembly (chromosome synapsis), and crossover recombination are essential for

104 The reaction involves DNA binding, synapsis, and cleavage at two RSSs located on the same D

105 chromosomes during meiosis requires pairing, synapsis, and crossing-over.

106 some segregation requires homologue pairing, synapsis, and crossover recombination, which occur durin

107 fect key prophase processes such as pairing, synapsis, and homologous recombination.

108 re behavior, meiotic recombination, pairing, synapsis, and installation of the meiosis-specific cytos

109 on of chromosomal events, including pairing, synapsis, and recombination.

110 ing homologous chromosome (homolog) pairing, synapsis, and recombination.

111 mportant for normal meiotic homolog pairing, synapsis, and repair of double-stranded breaks.

112 rgue that BLM is involved in proper pairing, synapsis, and segregation of homologous chromosomes; how

113 of the timing and progression of chromosome synapsis, and the gradual release of the individual cent

114 Chromosome synapsis appears to proceed normally, without significan

115 Homologous chromosome pairing and synapsis are prerequisite for accurate chromosome segreg

116 ochromatin, as well as incomplete chromosome synapsis associated with persistent RAD51 foci and gamma

117 rstitial ZYP1 loci elongating at zygotene so synapsis at centromeres is a continuation of the interst

118 In many organisms, homolog pairing and synapsis at meiotic prophase depend on interactions betw

119 lacking SIX6OS1 are defective in chromosome synapsis at meiotic prophase I, which provokes an arrest

120 gotene are essential steps before chromosome synapsis at pachytene.

121 This structure facilitated S-S synapsis because Smicro was proximal to Emicro and a dow

122 the discovery of a checkpoint that monitors synapsis between homologous chromosomes to ensure accura

123 normal prophase program of recombination and synapsis between homologous chromosomes, including loadi

124 ut does not require double-strand breaks for synapsis between homologous chromosomes.

125 ood but may have a critical role in ensuring synapsis between homologs and regulating double-strand b

126 meiosis in budding yeast and mammals is that synapsis between homologs depends upon recombination; ho

127 rrelates with the elongation of interstitial synapsis between the corresponding homologues.

128 rmation of the synaptonemal complex (SC), or synapsis, between homologs in meiosis is essential for c

129 just one copy of a chromosome pair promotes synapsis but does not support synapsis-independent pairi

130 rate-limiting step of excision occurs after synapsis, but closely precedes or is concomitant with th

131 Male Drosophila lack synapsis, but nonetheless, their chromosomes closely ass

132 The allosteric effect of GTP in promoting synapsis by P element transposase may be to orient a sec

133 Here we show that CHK-2 promotes pairing and synapsis by phosphorylating a family of zinc finger prot

134 negatively regulate synapsis and promote the synapsis checkpoint response.

135 tant assembles aberrant SC that triggers the synapsis checkpoint.

136 ggests that DSBs are being repaired in these synapsis-defective mutants.

137 The chromosome synapsis defects and Prophase I apoptosis of Pol beta-de

138 ing is genetically separable from subsequent synapsis, defined as stabilization of pairing by the syn

139 ans, synapsis and a checkpoint that monitors synapsis depend on pairing centers (PCs), cis-acting loc

140 In C. elegans, homolog pairing and synapsis depend on pairing centers (PCs), special region

141 However, 12/23-regulated synapsis does not strongly stimulate the cleavage activi

142 mechanisms, induces directional movement and synapsis driven by the machinery responsible for recombi

143 morphological changes required for homologue synapsis, DSB repair, and meiotic chromosome segregation

144 architectural scaffolding that promotes S-S synapsis during CSR and that these interactions are stab

145 lays an important role in initiating homolog synapsis during meiosis in Drosophila females.

146 t these sites mediate chromosome pairing and synapsis during meiosis, and that each site contains bin

147 completely disrupted chromosome pairing and synapsis during meiosis.

148 hich are essential for homologous chromosome synapsis during meiosis.

149 nd this is associated with severe defects in synapsis during the first meiotic division and reduced m

150 Chromosome synapsis during zygotene is a prerequisite for the timel

151 As short stretches of subtelomeric synapsis emerged at early zygotene, centromere clusters

152 Telomere bouquet formation is normal, but synapsis fails and oocytes accumulate in large numbers a

153 tosis during midpachytene of male meiosis if synapsis fails.

154 step of HR, while RSC is required following synapsis for completion of the recombinational repair ev

155 data support a model of capture rather than synapsis for pairwise RSS cleavage during V(D)J recombin

156 nstrate its combination with FRET to observe synapsis formation by Cre using excitation by a single l

157 elomere bouquet followed by the extension of synapsis from telomeres at the base of the bouquet, thus

158 sence of GTP or nonhydrolyzable GTP analogs, synapsis happens rapidly, whereas DNA cleavage is slow.

159 s structurally predicted to be important for synapsis, have been generated and characterized.

160 ome X chromosomes achieve nonhomologous self-synapsis; however, germ cells with SYP-1-positive X chro

161 The observed level of synapsis implies that Ph1 functions to promote homologue

162 esion, is required for homologue pairing and synapsis in budding yeast.

163 is-specific protein essential for chromosome synapsis in budding yeast.

164 inating establishment of homolog pairing and synapsis in C. elegans and provide evidence that checkpo

165 some sites that are also required to promote synapsis in Caenorhabditis elegans.

166 We investigated the genetic requirements for synapsis in Drosophila and found that there are three te

167 Extensive nonhomologous synapsis in htp-1; chk-2 double mutants indicates that H

168 the Mre11 complex in meiotic DNA repair and synapsis in mammals and indicate that the complex may co

169 s revealed incomplete chromosome pairing and synapsis in meiotic prophase, and extensive chromosome f

170 bservations can be well explained by ectopic synapsis in NAHR together with our proposed model of chr

171 ybl1(repro9)) had subtle defects in autosome synapsis in pachynema, a high incidence of unsynapsed se

172 ccordingly, we recently initiated a study of synapsis in the human male, combining immunofluorescence

173 fy the protein-DNA interactions that lead to synapsis in the Tn5 system.

174 me axes and about the spatial progression of synapsis in three dimensions.

175 s suggest a multistep pathway for chromosome synapsis in which PCs impart selectivity and efficiency

176 -6 mutants partially restores the defects in synapsis, in agreement with FKB-6 acting by decreasing c

177 among organisms with limited sex chromosome synapsis, including mammals.

178 e that the probability of ectopic chromosome synapsis increases with increased LCR length, and that e

179 defects in homologous chromosome pairing and synapsis independent of DNA damage per se.

180 nto distinct loop domains and inhibiting RSS synapsis, independent of any effects on transcription, R

181 pair promotes synapsis but does not support synapsis-independent pairing stabilization, indicating t

182 PCs usually fail to synapse and also lack a synapsis-independent stabilization activity.

183 budding yeast have suggested that chromosome synapsis initiates at the sites of crossing over.

184 Os and SYP-1 protein support models in which synapsis initiates predominantly in the vicinity of pair

185 ial associations, where proteins involved in synapsis initiation are located.

186 Surprisingly, synapsis initiation at centromeres is independent of the

187 Fpr3 and Zip3 appear to specifically prevent synapsis initiation at centromeric sites.

188 e previously characterized components of the synapsis initiation complex.

189 w that without Fpr3 and Zip3 activities, the synapsis initiation components Zip2 and Zip4 are dispens

190 Surprisingly, late zygotene synapsis initiation events are independent of the earlie

191 he Zip3 protein, which plays a major role in synapsis initiation events at noncentromeric locations.

192 d-zygotene events, whereas both mid and late synapsis initiation events depend on the cohesin subunit

193 Our data provide evidence for two classes of synapsis initiation events that differ in location, timi

194 eric chromatin coinciding with key events of synapsis initiation from the subtelomeric regions.

195 n in regions distant from prominent sites of synapsis initiation, and CO-inhibitory role(s) that limi

196 ormation, the roles of centromere pairing in synapsis initiation, and the mechanisms by which oocytes

197 emporally and genetically distinct stages of synapsis initiation.

198 centromeric regions are preferred sites for synapsis initiation.

199 ing facilitates homolog pairing and promotes synapsis initiation.

200 segments, could potentially be mediated by a synapsis intermediate involving an intergenic parallel-s

201 se IV NHEJ ligation complex, that end-to-end synapsis involves a dynamic positioning of the two ends

202 with increased LCR length, and that ectopic synapsis is a necessary precursor to ectopic crossing-ov

203 Our results indicate that synapsis is a tightly regulated process, with relatively

204 Early end synapsis is associated with kinase autophosphorylation.

205 le for homologous pairing and continue until synapsis is completed.

206 TIVE51 (RAD51) foci are largely reduced, and synapsis is completely abolished in dsy2 meiocytes.

207 kpoint-like manner to ensure that chromosome synapsis is contingent on the initiation of recombinatio

208 ank and, unexpectedly, the RSS spacer, while synapsis is controlled primarily by the RSS nonamer.

209 Once initiated, synapsis is highly processive, even between nonhomologou

210 for meiotic chromosome segregation, but how synapsis is initiated between chromosomes is poorly unde

211 S-S synapsis is mediated by a chromatin loop that spans the

212 one end of a linear stretch, suggesting that synapsis is often unidirectional.

213 In "early zygotene" oocytes, synapsis is only observed at the centromeres.

214 Homologue synapsis is required for meiotic chromosome segregation,

215 ted DNA cleavage before or immediately after synapsis is required to stabilize the synaptic assemblie

216 Synapsis is the process by which paired chromosome homol

217 ent to the nuclear envelope and for post-DSB synapsis, is also required for early pre-DSB homolog pai

218 We propose that once pairing or synapsis juxtaposes homologues, exclusion of Mek1 is nec

219 equence important for chromosome pairing and synapsis may be the culprit.

220 in which isolated RAG-RSS complexes undergo synapsis mediated by RAG protein-protein interactions.

221 ay be involved in non-homologous chromosomal synapsis, meiotic sex chromosome inactivation, and XY bo

222 Synapsis must therefore be directed solely by integrase-

223 ins that is also highlighted in a screen for synapsis mutants.

224 are absent--Ph1 affects neither the level of synapsis nor the number of MLH1.

225 rtial pairing, recombination initiation, and synapsis occur in the absence of wild-type Rad50 catalyt

226 ends were in the wrong relative orientation, synapsis occurred exclusively by random collision.

227 When Fpr3 and Zip3 are absent, synapsis occurs even in a mutant that fails to initiate

228 However, synapsis occurs only between attP and attB, and not betw

229 We found that synapsis occurs with a high affinity (Kd = 10 nM) and is

230 not inhibited and generalized nonhomologous synapsis occurs.

231 Molecular mechanisms underlying synapsis of activation-induced deaminase (AID)-targeted

232 for this directionality is due to selective synapsis of attP and attB sites.

233 in the atm single mutant, prevents complete synapsis of chromosomes, and results in extensive and pe

234 Plectonemic intertwining favors only synapsis of closely linked transposon ends in the invert

235 critical step in V(D)J recombination is the synapsis of complementary (12/23) recombination signal s

236 53BP1 promotes CSR in part by mediating synapsis of distal DNA ends, and in addition, inhibits 5

237 hanges in locus conformation may control the synapsis of distant recombination signal sequences, and

238 ion and therefore suppresses the promiscuous synapsis of distant transposon ends, which initiate McCl

239 a vertebrate cell-free extract to show that synapsis of DNA ends occurs in at least two stages that

240 sonance energy transfer (FRET) to detect the synapsis of fluorescently labeled RSS oligonucleotides.

241 This is exemplified by improper pairing and synapsis of homologous chromosomes and altered processin

242 strand break repair which ensures the proper synapsis of homologous chromosomes and normal meiotic pr

243 Pairing and synapsis of homologous chromosomes during meiosis is cru

244 ble heterodimeric complex, ensure the proper synapsis of homologous chromosomes in meiosis by acting

245 of meiotic prophase, incomplete and aberrant synapsis of homologous chromosomes, persistence of stran

246 se integrase-bound attP and attB and inhibit synapsis of integrase-bound attL and attR.

247 ement for a specific orientation of Mu ends, synapsis of L and R was entirely by interwrapping.

248 tive Cre K201A mutant is fully competent for synapsis of loxP sites, yet the inactive Y324F and R173K

249 have studied the energetics of Cre-mediated synapsis of loxP sites.

250 A key stage in meiosis is the synapsis of maternal and paternal homologous chromosomes

251 se plants reveal reduced homologous pairing, synapsis of nonhomologous chromosomes, reduced bivalents

252 on attL and attR recombination partners, and synapsis of partner complexes follows rapidly after thei

253 recombinase N-terminal domain, required for synapsis of recombination substrates and catalysis.

254 cells, chromosomes still hypercondense, but synapsis of sister chromatids is abolished.

255 usly we reported that Tn3 resolvase-mediated synapsis of the accessory binding sites from the Tn3 rec

256 core of a multiprotein complex that promotes synapsis of the broken DNA ends.

257 is a highly concerted process that requires synapsis of the correct pair of DNA substrates.

258 this suggests that processing occurred after synapsis of the ends.

259 ene stage of meiotic prophase with defective synapsis of the homologous chromosomes.

260 g of homologous chromosomes and the intimate synapsis of the paired homologs by the synaptonemal comp

261 chestrates a regulatory mechanism to enforce synapsis of the transposon ends before cleavage by the t

262 We also show that synapsis of the transposon ends is a prerequisite for th

263 ves to downregulate transposition by slowing synapsis of the transposon ends.

264 osis polymerase domain of LigD mediating the synapsis of two noncomplementary DNA ends revealed a var

265 a substrates and to some extent through poor synapsis of Vbeta and Jbeta substrates.

266 re of meiosis, mediating the stable pairing (synapsis) of homologous chromosomes during prophase I.

267 ecombination reaction is the association, or synapsis, of Cre-bound loxP sites to form a tetrameric p

268 When either synapsis or crossover formation is impaired, CHK-2 activ

269 75 phosphorylation does not alter chromosome synapsis or DSB repair, indicating that Mec1 separates c

270 s including incomplete homologous chromosome synapsis or persistent histone H2AX phosphorylation in f

271 omal replication, sister chromatid cohesion, synapsis or recombination.

272 s to conclude that RAG binding, bending, and synapsis precede catalysis.

273 modification of homologous recombination and synapsis, probably via adjustments of core structural co

274 mutant indicate that chromosome pairing and synapsis proceed with normal distribution of the early r

275 s of asymmetry along chromosomes are lost in synapsis-proficient crossover-defective mutants, which o

276 escued the fertility of oocytes containing a synapsis-proficient, DSB repair-defective mutation in a

277 As synapsis progresses and linear stretches of Zip1 are for

278 microscopy (3D-SIM), we observed that normal synapsis progression was also disrupted in des10, a phen

279 leavage and effects on resolvase binding and synapsis, providing insight into the serine recombinase

280 ms in which recombination initiates prior to synapsis, recombination preferentially occurs in short 1

281 ultiprotein complexes that are essential for synapsis, recombination, and segregation of homologous c

282 osomal escape from MSCI without accompanying synapsis/recombination defects.

283 We find that XYY males, like synapsis/recombination mutants, display pachytene arrest

284 Our computer simulation of the synapsis showed that the bend angles, phi, created in is

285 n meiosis and into the mechanisms regulating synapsis so that it occurs selectively between homologs.

286 es Tdp1, and a second step that occurs after synapsis that requires Exo1.

287 For both types of chromosomes, synapsis then proceeds toward the centromere, with littl

288 nisms eliminate meiotic cells with defective synapsis, thereby minimizing transmission of aneuploidy.

289 keleton mediate homolog pairing and restrict synapsis to homologous pairs of chromosomes.

290 ssing-over, functioning to couple chromosome synapsis to the formation of crossover-specific recombin

291 ion of the enhancer to this system channeled synapsis toward the interwrapped pathway.

292 hen combined with a quantitative analysis of synapsis using loxP mutants, the structures explain how

293 unction for Pol beta in recombination and/or synapsis, we used conditional gene targeting to delete t

294 condensation is a key factor in stimulating synapsis, whereas decondensation may facilitate the inva

295 howed a significant defect in sex chromosome synapsis, which likely contributed to the germ cell loss

296 al relationships between DNA target sites at synapsis, which we investigate using nicked-circular DNA

297 some IV sequences are capable of pairing and synapsis, while the contiguous X portion of mnT12 lacks

298 translocation and find that mcr can undergo synapsis with a standard recombination signal sequence w

299 conformation on binding to attB that permits synapsis with attP.

300 sets displayed a mix of synaptic failure and synapsis with both homologous and nonhomologous partners