ライフサイエンスコーパス: synaptobrevin

1 sicle associated membrane protein (VAMP/also Synaptobrevin).

2 mal-associated protein of 23 or 25 kDa), and synaptobrevin.

3 1 binds specifically and non-specifically to synaptobrevin.

4 to identify positive regulators of vesicular synaptobrevin.

5 ns such as epsin, protein kinase C-beta, and synaptobrevin.

6 rodimers, leading to an acceptor complex for synaptobrevin.

7 as been artificially depleted by deletion of synaptobrevin.

8 te and colocalizes with the synaptic marker, synaptobrevin.

9 s transmembrane helix proteins: syntaxin and synaptobrevin.

10 protein vesicle-associated membrane protein/synaptobrevin.

11 SNAP-25 and Syntaxin1, the SNARE partners of synaptobrevin.

12 r regions of the vesicle-associated protein, synaptobrevin.

13 imulations with an external force applied to synaptobrevin.

14 hment protein-25 (SNAP-25), syntaxin 1A, and synaptobrevin.

15 to the interaction between UNC-17/VAChT and synaptobrevin.

16 nesis) and synaptophysin, PSD95, synapsin 1, synaptobrevin 1, neurogranin, GAP43 and synaptopodin (sy

17 d synaptic-synaptophysin, PSD95, synapsin 1, synaptobrevin 1, neurogranin, GAP43 and synaptopodin in

18 , C2F did not bind the vesicle SNARE protein synaptobrevin-1 (VAMP-1).

19 oprecipitated syntaxin-1 and SNAP-25 but not synaptobrevin-1.

20 actor attachment protein receptor) proteins: synaptobrevin 2 (or vesicle-associated membrane protein

21 Interactions between synaptobrevin 2 (Sb2) and syntaxin 1A (Sx1A) can be read

22 e-associated protein of 25 kDa (SNAP25), and synaptobrevin 2 (Sb2).

23 on of the SNARE complex by the vesicle SNARE synaptobrevin 2 (syb2) and the two plasma membrane SNARE

24 The neuronal vesicular SNARE protein synaptobrevin 2 (syb2) is anchored in the vesicle membra

25 protein attachment protein receptor (SNARE) Synaptobrevin 2 (Syb2) is known for mediating neurotrans

26 domain (TMD) of the vesicle membrane protein synaptobrevin 2 (syb2).

27 unilamellar vesicles doped with the v-SNARE synaptobrevin 2 by means of spinning-disc confocal micro

28 For example, the binary syntaxin-synaptobrevin 2 complex, in addition to the ternary comp

29 nts in astrocytes expressing the fluorescent synaptobrevin 2 derivative, synapto-pHluorin.

30 Synaptobrevin 2 is thought to facilitate fusion of synap

31 Thus, synaptobrevin 2 may function in catalyzing fusion reacti

32 fected with green fluorescent protein-tagged synaptobrevin 2, a marker of synaptic vesicles.

33 ensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-seri

34 complexes that include the vesicular SNARE, synaptobrevin 2, and that the participation of 5RK in CD

35 tion on its substratum, the synaptic protein synaptobrevin 2, attached to the beads.

36 t the ionic layer by cuffing syntaxin 1A and synaptobrevin 2, similar to the action of SNAP25B; thus

37 In the absence of synaptobrevin 2, spontaneous synaptic vesicle fusion and

38 In contrast, the apparent number of VAMP2/synaptobrevin 2, synaptophysin, and synaptogyrin demonst

39 rotein receptor (SNARE) complex, composed of synaptobrevin 2, syntaxin and synaptosome-associated pro

40 achment protein receptors complex, including synaptobrevin 2, syntaxin, and synaptosome-associated pr

41 ent protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key ste

42 etween two synaptic proteins syntaxin 1A and synaptobrevin 2, using an atomic force microscope and th

43 in FcepsilonRI-regulated exocytosis, whereas synaptobrevin 2- or VAMP-3-deficient mast cells did not.

44 2 (Delta324-339), block its interaction with synaptobrevin-2 (L348R), or extend the helix to promote

45 Addition of the V(C) peptide synaptobrevin-2 (syb(57-92)) increases the docking effic

46 erated four variants of the synaptic v-SNARE synaptobrevin-2 (syb2) anchored to the membrane by lipid

47 tosomal-associated protein 25 (SNAP-25), and synaptobrevin-2 (Syb2).

48 complex composed of SNAP-25, syntaxin-1, and synaptobrevin-2 (sybII) proteins.

49 b(G76V), GFP, and a synaptic vesicle protein synaptobrevin-2 (Ub(G76V)-GFP-Syb2); (2) GFP-Syb2; or (3

50 cholesterol on fusion pore formation between synaptobrevin-2 (VAMP-2)-containing proteoliposomes and

51 naptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasm

52 hen bound to Munc18-1, preventing binding to synaptobrevin-2 and SNAP-25 to form the ternary SNARE co

53 Using recombinant fragments of synaptobrevin-2 and synaptotagmin C2 domains we were abl

54 sealing response was blocked by fragments of synaptobrevin-2 and the C2B domain of synaptotagmin VII.

55 nt on interactions between the vesicle SNARE synaptobrevin-2 and the plasma membrane SNAREs syntaxin-

56 ly due to fewer vesicles of reduced size and synaptobrevin-2 content.

57 omplexin interaction reduces the affinity of synaptobrevin-2 for the 1:1 complex, thereby retarding S

58 SPalpha), synaptophysin, synaptotagmin-1, or synaptobrevin-2 in their axons.

59 al neurons, but not in neurons obtained from synaptobrevin-2 knockout mice.

60 lipid-anchored syntaxin-1 and lipid-anchored synaptobrevin-2 lacking TMRs efficiently promoted sponta

61 y fluorescent tagging of the vesicle protein synaptobrevin-2 or by staining with the styryl dye FM4-6

62 n docking, but little effect on the rates of synaptobrevin-2 proteoliposome fusion.

63 Examining single particle fusion between synaptobrevin-2 proteoliposomes and planar-supported bil

64 These observations provide evidence that the synaptobrevin-2 TMD catalyzes the fusion process by its

65 , we show that conformational flexibility of synaptobrevin-2 TMD is essential for efficient Ca(2+)-tr

66 he absence of synaptic vesicle SNARE protein synaptobrevin-2 whereas the increase in spontaneous fusi

67 BPB also quenched fluorescence of VAMP (synaptobrevin-2)-EGFP, thus indicating the timing of fir

68 aptic SNARE proteins SNAP-25, syntaxin-1 and synaptobrevin-2, as well as by an age-dependent reductio

69 s as tetanus toxin did not cleave astrocytic synaptobrevin-2, nor was AA released from pure astrocyte

70 SNARE complex consists of the three proteins synaptobrevin-2, syntaxin, and synaptosomal-associated p

71 uxtamembranous mutation in the SNARE-protein synaptobrevin-2, which presumably impairs force transfer

72 contained synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha immunoreactivity, respect

73 Among synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha-IR varicosities, 98% +/-

74 Our present results show that synaptobrevin-2-like immunoreactivity (-LIR) is present

75 ste cells with synapses display SNAP-25- and synaptobrevin-2-like immunoreactivity (LIR).

76 Synaptobrevin-2-LIR also colocalizes with immunoreactivi

77 Synaptobrevin-2-LIR colocalizes with SNAP-25-, serotonin

78 old immunoelectron microscopy, we found that synaptobrevin-2-LIR is associated with synaptic vesicles

79 However, approximately 27% of the synaptobrevin-2-LIR taste cells do not display IP3R3-LIR

80 rom taste cells onto nerve processes express synaptobrevin-2-LIR, as well as some taste cells without

81 oth type II and type III taste cells display synaptobrevin-2-LIR.

82 All IP3R3-LIR taste cells express synaptobrevin-2-LIR.

83 ranes that kinetically alters the binding of synaptobrevin-2.

84 lex result in an additional interaction with synaptobrevin-2/VAMP2 (vesicle-associated membrane prote

85 ractions of native alpha-synuclein with both synaptobrevin-2/VAMP2 and anionic lipids.

86 In contrast, the R-SNARE protein synaptobrevin-2/VAMP2 contributes to both regulated and

87 helix 12 in Munc18 within domain 3a leads to synaptobrevin-2/VAMP2 interaction and SNARE complex form

88 n complexes composed of syntaxin-1, SNAP-25, synaptobrevin-2/VAMP2, and Munc18-1.

89 amma-synuclein was not able to interact with synaptobrevin-2/VAMP2.

90 ynuclein directly bound to the SNARE-protein synaptobrevin-2/vesicle-associated membrane protein 2 (V

91 We monitored the distribution of synaptobrevin, a vesicle protein required for exocytosis

92 b(G76V)-GFP-Syb2 may compete with endogenous synaptobrevin, acting as a gain-of-function mutation tha

93 vere decrease in exocytosis than deletion of synaptobrevin alone.

94 The synaptic vesicle protein synaptobrevin (also called VAMP, vesicle-associated memb

95 ctor attachment protein receptors) proteins: synaptobrevin (also known as VAMP) on the synaptic vesic

96 ing of individual RGC axons coexpressing GFP-synaptobrevin and DsRed in the intact Xenopus brain demo

97 r vesicle-associated membrane protein (VAMP)/synaptobrevin and found reduced neural-crest-derived cel

98 a conserved set of SNARE proteins: vesicular synaptobrevin and plasma membrane syntaxin and SNAP-25.

99 rization of structural elements in prefusion synaptobrevin and providing a framework for interpreting

100 s including increased levels of synapsin and synaptobrevin and reduced levels of NMDA receptor subuni

101 ocking/fusion machinery that binds with VAMP/synaptobrevin and SNAP-25 to form the SNARE complex.

102 s on the SNARE complex formed by syntaxin-1, synaptobrevin and SNAP-25, as well as on complexins, whi

103 ARE helix is available for interactions with Synaptobrevin and SNAP-25.

104 brium the Cpx AH forms tight links with both synaptobrevin and SNAP25.

105 ic complexes, after stimulation up to 25% of synaptobrevin and synaptophysin are present in homo- and

106 Whereas at rest less than 10% of the total synaptobrevin and synaptophysin could be chemically cros

107 evealed selective reduction of the levels of synaptobrevin and synaptophysin in synaptosomes from stg

108 ." MISTs based on the vesicle proteins VAMP2/Synaptobrevin and Synaptophysin induced rapid ( approxim

109 but represent distinct functional states of synaptobrevin and synaptophysin that are modulated in pa

110 Donor vesicles contain reconstituted synaptobrevin and synaptotagmin-1.

111 The expression of synaptobrevin and syntaxin 3, TA proteins essential for

112 lical conformation to the groove between the synaptobrevin and syntaxin helices.

113 propensities of the transmembrane domains of synaptobrevin and syntaxin.

114 es form between the synaptic vesicle protein synaptobrevin and the plasma membrane proteins syntaxin

115 The synaptic vesicle SNARE synaptobrevin and the plasma membrane SNAREs syntaxin-1

116 " by fusion to the transmembrane segments of synaptobrevin and to a lesser extent, synaptotagmin.

117 as been proposed that Munc18-1 also binds to synaptobrevin and to the SNARE four-helix bundle and tha

118 ow demonstrate that Munc18-1 indeed binds to synaptobrevin and to the SNARE four-helix bundle.

119 es required diaphanous, SCAR, Neuroglian and Synaptobrevin, and both the Hh gradient and Hh signaling

120 interactions among syntaxin, SNAP23/SNAP25, synaptobrevin, and complexin by employing a newly develo

121 7, choline acetyltransferase, Synaptotagmin, Synaptobrevin, and RAB-3) are substantially reduced in u

122 The SNAREs Syntaxin-1, Synaptobrevin, and SNAP-25 play a central role in membra

123 Futsch, a marker of neurites, and Synapsin, Synaptobrevin, and Synaptogamin, proteins involved in ne

124 onal SNARE complexes consisting of syntaxin, synaptobrevin, and synaptosome-associated protein of 25

125 evels of the vesicle proteins synaptophysin, synaptobrevin, and synaptotagmin, without affecting the

126 three SNARE proteins, syntaxin, SNAP-25, and synaptobrevin, and the SM protein, Munc18-1.

127 25), and vesicle-associated membrane protein/synaptobrevin are collectively called SNAP receptor (SNA

128 ilt and insertion depth of membrane-embedded synaptobrevin are determined.

129 s involving the SNARE motifs of syntaxin and synaptobrevin as well as those of syntaxin and synaptoso

130 ARE protein machinery syntaxin, SNAP-25, and synaptobrevin, as is used by synapses in the central ner

131 Four mutations in the VAMP/synaptobrevin-associated protein B (VAPB) gene have been

132 VAMP/synaptobrevin-associated proteins (VAPs) contain an N-te

133 NARE complex assembly, and autoinhibition of synaptobrevin binding contributes to enabling regulation

134 n reduce synaptotagmin, alpha/beta-SNAP, and synaptobrevin binding.

135 f of the SNARE motif from the neuronal SNARE synaptobrevin binds to membranes, which appeared to cont

136 or of vesicle docking with only syntaxin and synaptobrevin, but have been confirmed by other experime

137 Munc18-1 binds to synaptobrevin, but the relevance of this interaction and

138 the TM helix in the simulation detached the synaptobrevin C-terminus from the vesicle's inner-leafle

139 h the Cpx AH clamps fusion by binding to the synaptobrevin C-terminus, thus preventing full SNARE zip

140 heless, by a single amino acid substitution, synaptobrevin can be driven to dimerize with the same af

141 NMDA receptor blockade: BDNF maintained GFP-synaptobrevin cluster density by maintaining their addit

142 gonists APV or MK801 transiently induced GFP-synaptobrevin cluster dismantling, but did not significa

143 ocking antibodies significantly enhanced GFP-synaptobrevin cluster elimination, a response that was p

144 ds along the groove between the syntaxin and synaptobrevin coils.

145 cellubrevin rescues synaptic transmission in synaptobrevin-deficient neurons but that deletion of bot

146 ue insertion restored spontaneous release in synaptobrevin-deficient neurons.

147 yt) strongly stimulated membrane fusion when synaptobrevin densities were similar to those found in n

148 the SNARE motif did not significantly impair synaptobrevin-dependent exocytosis, whereas insertion of

149 tants, we found that the majority of surface synaptobrevin derives from fusion of synaptic vesicles a

150 stimate a dissociation constant of 10 mM for synaptobrevin dimerization in detergent.

151 nd that the individual proteins syntaxin and synaptobrevin disrupt membranes so as to favor formation

152 ns but that deletion of both cellubrevin and synaptobrevin does not cause a more severe decrease in e

153 The synaptic vesicle protein synaptobrevin engages with syntaxin and SNAP-25 to form

154 synaptic vesicles and that, in steady state, synaptobrevin equilibrates throughout the axon.

155 a synaptic vesicle-associated protein termed synaptobrevin, exhibit similar convulsion phenotypes fol

156 The increase in synaptophysin and synaptobrevin expression was moderate (2-fold) and occur

157 ompetes with the SNARE four-helix bundle and synaptobrevin for Munc18-1 binding.

158 monstrate that FTY720 can activate vesicular synaptobrevin for SNARE complex formation and enhance ex

159 The SNARE proteins, syntaxin, SNAP-25 and synaptobrevin form a tertiary complex essential for vesi

160 In the SNARE complex, the SNARE motif of synaptobrevin forms a 55-residue helix, but it has been

161 Recombinant synaptobrevin forms a small amount of dimer and higher o

162 sing membranes, it does not act by releasing synaptobrevin from synaptic vesicle restriction.

163 legans motor neurons by using a pH-sensitive synaptobrevin GFP fusion protein, synaptopHluorin.

164 aptic vesicle clustering, as demonstrated by synaptobrevin-GFP fluorescent imaging, and FM dye staini

165 Direct visualization of Synaptobrevin-GFP transport in living animals demonstrat

166 synaptic vesicle markers (Synaptotagmin and Synaptobrevin-GFP) and clear-core vesicles along Drosoph

167 First, we demonstrate that a synaptobrevin-GRASP chimera functions as a powerful acti

168 esults show that the transmembrane domain of synaptobrevin has only a modest ability to self-associat

169 A syntaxin/synaptobrevin heterodimer is able to form under oxidizin

170 In contrast to previous studies, we found synaptobrevin homodimerization in E. coli is very weak w

171 To gauge the magnitude of synaptobrevin homodimerization, we used the well-charact

172 Live imaging of SYNA, a synaptobrevin homologue, and SECC, an exocyst component,

173 physin results in the defective retrieval of synaptobrevin II (sybII) from the plasma membrane during

174 membrane domain (JMD) of the vesicular SNARE Synaptobrevin II (SybII) profoundly impairs priming of g

175 er release is mediated by the SNARE proteins synaptobrevin II (sybII, also known as VAMP2), syntaxin,

176 Green fluorescent protein (GFP)-tagged synaptobrevin II served as a marker to visualize synapti

177 exocytosis, we expressed the SNARE motif of synaptobrevin II to prevent the formation of SNARE compl

178 Green fluorescent protein (GFP)-tagged synaptobrevin II was used to visualize presynaptic speci

179 complexes formed between the SNARE motifs of synaptobrevin II, SNAP-25, and syntaxin play an essentia

180 ponents of synaptic vesicles, such as VAMP 2/synaptobrevin II, synaptophysin, synaptotagmin, or prote

181 The three neuronal SNAREs syntaxin-1A, synaptobrevin-II (VAMP), and SNAP-25A were expressed sep

182 imulations of the prefusion configuration of synaptobrevin in a lipid bilayer, aimed at characterizin

183 evidence showing the dual role of SNAP25 and synaptobrevin in both exocytosis and slow endocytosis at

184 NMR data for full-length synaptobrevin in dodecylphosphocholine micelles reveals

185 However, the membrane-embedded structure of synaptobrevin in its prefusion state, which determines i

186 o contradict previous biophysical studies of synaptobrevin in liposomes.

187 Thus, the dimerization of synaptobrevin in membranes is very weak, questioning any

188 Syntaxin and synaptobrevin in opposing membranes were both necessary

189 protein surface concentrations reported for synaptobrevin in synaptic vesicles and with an optimally

190 easable backbone of sphingolipids, activates synaptobrevin in synaptic vesicles to form the SNARE com

191 , as well as evidence for gene expression of synaptobrevin in the lamellar cells led to the hypothesi

192 ng vesicles could mix, suggesting a role for synaptobrevin in the separation of the two pools.

193 paired recordings, we find that cleavage of synaptobrevin in unprimed vesicles leads to an eventual

194 Consistent with the role of synaptobrevin in vesicle fusion, sphingosine upregulated

195 synapses lacking the vesicular SNARE protein synaptobrevin in which synaptic responses are severely d

196 ast, cleavage of the synaptic vesicle SNARE (synaptobrevin) in conjunction with deletion of the vesic

197 other conformational states of the syntaxin*synaptobrevin interaction in addition to those observed

198 mes reconstituted with full-length v-SNAREs (synaptobrevin) into planar lipid bilayers containing bin

199 axin 1A and SNAP-25 with the vesicle protein synaptobrevin is a critical step in neuronal exocytosis.

200 Synaptobrevin is a vesicle-associated membrane protein (

201 most of the SNARE motif of membrane-anchored synaptobrevin is accessible for SNARE complex formation.

202 d heteromultimerization of synaptophysin and synaptobrevin is increased up to 6-fold.

203 ues, we found that not only SNAP25, but also synaptobrevin is involved in slow endocytosis.

204 Importantly, the Munc18-1 binding site on synaptobrevin is located at the C-terminus of its SNARE

205 osslinking studies show that dimerization of synaptobrevin is promoted by oxidizing agents.

206 ciated membrane protein (VAMP; also known as synaptobrevin) is a key component of the core complex ne

207 complex with the core proteins, SNAP-25 and synaptobrevin, is also required for the association with

208 educed the number of red fluorescent protein-synaptobrevin-labeled presynaptic specializations per ax

209 ition enables syntaxin and SNAP-25 to engage synaptobrevin, leading to membrane fusion.

210 ase, suggesting that intact synaptobrevin or synaptobrevin-like molecules are required for exocytosis

211 nredundant functions in the recycling of the synaptobrevin-like v-SNARE Snc1 from early endosomes.

212 sults in only mild and occasional defects in synaptobrevin localization.

213 Our data suggest that synaptobrevin mediates Ca2+-triggered exocytosis by tigh

214 taxin 1A and SNAP-25 and the vesicle v-SNARE synaptobrevin, mediates the fusion of 2 membranes.

215 The stimulus dependence of synaptophysin and synaptobrevin multimers indicates that the complexes are

216 eins involved in the same molecular process (synaptobrevins, Munc13-1/2) do not cause degeneration.

217 ene family: synaptobrevin (syb) and neuronal synaptobrevin (n-syb).

218 for SNARE complex formation, availability of synaptobrevin on synaptic vesicles is very limited.

219 nd SNAP-25 on the target plasma membrane and synaptobrevin on the vesicular membrane.

220 nhibits NT-3 release, suggesting that intact synaptobrevin or synaptobrevin-like molecules are requir

221 Synaptobrevins or VAMPs are vesicle-associated membrane

222 disrupts the interaction of the Cpx AH with synaptobrevin, partially imitating the cpx null phenotyp

223 nsights suggest that sphingosine acts on the synaptobrevin/phospholipid interface, defining a novel f

224 on-specific synaptic v-SNARE n-syb (neuronal Synaptobrevin) plays a key role during synaptic vesicle

225 ed rats had higher density of synaptophysin-/synaptobrevin-positive puncta in DG and CA1 subregions o

226 NMR analyses of synaptobrevin reconstituted into nanodiscs and into lipo

227 the transmembrane sequences of syntaxin and synaptobrevin reveal structural models that correlate wi

228 d membrane protein type v-SNARE proteins (or synaptobrevins) reveals characteristic alterations to ve

229 o suspend the bead off the cantilever we use synaptobrevin's molecular interaction with another synap

230 m hemifusion to complete fusion, the role of synaptobrevin's TM domain association in the fusion proc

231 ic SNARE protein where a portion of neuronal synaptobrevin (Sb) is fused to Snc2p, a Sb ortholog requ

232 ol (PEG) to investigate the influence of the synaptobrevin (SB) TMD with an attached JMR (SB-JMR-TMD)

233 SNARE complex, which comprises the proteins synaptobrevin, SNAP-25 and syntaxin.

234 cytosis machinery, including SNARE proteins (synaptobrevin, SNAP25, and syntaxin), is needed to coini

235 SNARE complex lacking the C-terminus of the synaptobrevin SNARE motif (SNAREDelta60) suggested that

236 by NMR experiments showing that the soluble synaptobrevin SNARE motif does not bind to liposomes, ev

237 ne anchoring, our data show that most of the synaptobrevin SNARE motif has a remarkable reluctance to

238 naptosomal-associated protein of 25 kDa, and synaptobrevin soluble N-ethylmaleimide sensitive factor

239 t of Munc18: it promotes the proper syntaxin/synaptobrevin subconfiguration during assembly of the te

240 o characterized members of this gene family: synaptobrevin (syb) and neuronal synaptobrevin (n-syb).

241 , fragments of the toxin substrate proteins, synaptobrevin (Syb) or synaptosome-associated protein of

242 s indicated that the vesicular SNARE protein synaptobrevin (syb) was dispensable for docking.

243 naptogenesis, namely Synaptophysin (Syn) and Synaptobrevin (Syb).

244 Synaptobrevin (Syb)/vesicle-associated membrane protein

245 Two homologous isoforms of synaptobrevin, Syb1/VAMP1 and Syb2/VAMP2, exhibit distin

246 Synaptobrevin (sybII) is another abundant integral SV pr

247 process-the vesicle-associated proteins VAMP/synaptobrevin, synaptotagmin, and Rab3-are each immobile

248 We propose that synaptobrevin, synaptotagmin, and UNC-104 are specific c

249 ptic vesicle-related proteins, such as VAMP (synaptobrevin), syntaxin-1, synaptophysin, synapsin Ia/b

250 The neuronal SNARE complex formed by synaptobrevin, syntaxin and SNAP-25 plays a central role

251 e formation of the SNARE complexes involving synaptobrevin, syntaxin and SNAP-25 that play an essenti

252 cysteine residue in the same location as in synaptobrevin, syntaxin dimerization is not promoted by

253 evious reports have described a homodimer of synaptobrevin that is dependent on the transmembrane dom

254 that there is a plasma membrane reservoir of synaptobrevin that is supplied by the synaptic vesicle c

255 0 nm) liposomes reconstituted with different synaptobrevin (the SNARE present in synaptic vesicles) d

256 ural properties of both wild-type and mutant synaptobrevin, the effects of C-terminal additions on ti

257 We study the oligomerization of synaptobrevin TMD using ensembles of molecular dynamics

258 hen examined the structural requirements for synaptobrevin to function in exocytosis.

259 ransmembrane region abolished the ability of synaptobrevin to mediate Ca2+-evoked exocytosis.

260 ynaptopHluorin created by the fusion of VAMP/synaptobrevin to the pH-sensitive super-ecliptic green f

261 Additionally, the synaptobrevin transmembrane domain can promote lipid mix

262 The synaptobrevin transmembrane domain has an alpha-helical

263 say to reexamine the homodimerization of the synaptobrevin transmembrane domain in detergents and the

264 ports have been presented on the role of the synaptobrevin transmembrane domain in mediating importan

265 semble the homodimer interface formed by the synaptobrevin transmembrane domain.

266 structure of a superclamp mutant bound to a synaptobrevin-truncated SNARE complex.

267 lexin-I accessory helix does not insert into synaptobrevin-truncated SNARE complexes in solution, and

268 directly fused to the presynaptic protein n-synaptobrevin, under the control of the pan-neuronal pro

269 in the absence of the vesicle SNARE protein synaptobrevin (VAMP), activity-dependent and spontaneous

270 or (SNARE) proteins syntaxin-1, SNAP-25, and synaptobrevin/VAMP (vesicle-associated membrane protein)

271 We generated knockout mice lacking synaptobrevin/VAMP 2, the vesicular SNARE protein respon

272 rotoxin requires the synaptic SNARE-proteins synaptobrevin/VAMP and SNAP-25, and, at least partly, th

273 Synaptophysin and synaptobrevin/VAMP are abundant synaptic vesicle protein

274 smitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-he

275 Vesicle Associated Membrane Protein (VAMPs: synaptobrevin/VAMP-2, cellubrevin/VAMP-3, TI-VAMP/VAMP-7

276 mice did express apparently higher levels of synaptobrevin/VAMP-2.

277 n-1, SNAP-25) or Munc18-1, but not v-SNAREs (synaptobrevins/VAMP1/2/3 using tetanus neurotoxin (TeNT)

278 e membrane insertion sequence of the v-SNARE synaptobrevin/vesicle-associated membrane protein (VAMP)

279 SNAP-25, syntaxin 1A, and synaptobrevin/vesicle-associated membrane protein (VAMP)

280 ptic vesicles at hippocampal synapses, using synaptobrevin/vesicle-associated membrane protein (VAMP)

281 Deletion of synaptobrevin/vesicle-associated membrane protein, the m

282 Docking and fusion of reconstituted synaptobrevin vesicles to target SNARE complex-containin

283 rane had no significant effect on docking of synaptobrevin vesicles.

284 The surface synaptobrevin was enriched near active zones, and its sp

285 hile the early increase in synaptophysin and synaptobrevin was not.

286 ease in multimerization of synaptophysin and synaptobrevin was only observed in intact but not in lys

287 We estimated that 30% of synaptobrevin was present in the plasma membrane.

288 The relative amount of fluorescent synaptobrevin was substantially lower at synapses of kno

289 s with only one synaptobrevin, whereas 23-30 synaptobrevins were necessary for efficient lipid mixing

290 s maximal with small liposomes with only one synaptobrevin, whereas 23-30 synaptobrevins were necessa

291 Hypomorphic mutations in syntaxin-1A or n-synaptobrevin, which also disrupt neurotransmitter relea

292 in receptors (SNAREs) SNAP-25, syntaxin, and synaptobrevin, which constitute part of the synaptic ves

293 s the SNARE proteins syntaxin-1, SNAP-25 and synaptobrevin, which form a tight "SNARE complex", and M

294 ronal SNARE proteins, SNAP-25, syntaxin, and synaptobrevin, which were reconstituted into vesicles.

295 t, highly tilted state for membrane-embedded synaptobrevin with a helical connection between the tran

296 ane domain, is markedly improved by a mutant synaptobrevin with an isoleucine-to-aspartate substituti

297 Cleavage of the other SNAREs (synaptobrevin with Botx/D or SNAP-25 with Botx/A) failed

298 vesicles bearing the vesicle fusion protein synaptobrevin with supported planar membranes harboring

299 Surprisingly, however, synaptobrevin with the 12-residue but not the 24-residue

300 osomes containing the synaptic vesicle SNARE synaptobrevin (with or without the Ca(2+) sensor synapto