ライフサイエンスコーパス: synaptobrevin 2

1 ranes that kinetically alters the binding of synaptobrevin-2.

2 fected with green fluorescent protein-tagged synaptobrevin 2, a marker of synaptic vesicles.

3 Syntaxin-1A, SNAP-25, and synaptobrevin-2 (also known as vesicle-associated membra

4 ed that V-ATPase subunit c is localized with synaptobrevin 2 and synaptophysin in synaptic vesicles.

5 naptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasm

6 hen bound to Munc18-1, preventing binding to synaptobrevin-2 and SNAP-25 to form the ternary SNARE co

7 Using recombinant fragments of synaptobrevin-2 and synaptotagmin C2 domains we were abl

8 sealing response was blocked by fragments of synaptobrevin-2 and the C2B domain of synaptotagmin VII.

9 nt on interactions between the vesicle SNARE synaptobrevin-2 and the plasma membrane SNAREs syntaxin-

10 ensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-seri

11 complexes that include the vesicular SNARE, synaptobrevin 2, and that the participation of 5RK in CD

12 contained synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha immunoreactivity, respect

13 Among synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPalpha-IR varicosities, 98% +/-

14 aptic SNARE proteins SNAP-25, syntaxin-1 and synaptobrevin-2, as well as by an age-dependent reductio

15 tion on its substratum, the synaptic protein synaptobrevin 2, attached to the beads.

16 unilamellar vesicles doped with the v-SNARE synaptobrevin 2 by means of spinning-disc confocal micro

17 For example, the binary syntaxin-synaptobrevin 2 complex, in addition to the ternary comp

18 ly due to fewer vesicles of reduced size and synaptobrevin-2 content.

19 nts in astrocytes expressing the fluorescent synaptobrevin 2 derivative, synapto-pHluorin.

20 BPB also quenched fluorescence of VAMP (synaptobrevin-2)-EGFP, thus indicating the timing of fir

21 omplexin interaction reduces the affinity of synaptobrevin-2 for the 1:1 complex, thereby retarding S

22 Anti-synaptobrevin 2 immunoprecipitates obtained from freshly

23 units, or synaptophysin, was present in anti-synaptobrevin 2 immunoprecipitates obtained from frozen-

24 SPalpha), synaptophysin, synaptotagmin-1, or synaptobrevin-2 in their axons.

25 Synaptobrevin 2 is thought to facilitate fusion of synap

26 al neurons, but not in neurons obtained from synaptobrevin-2 knockout mice.

27 2 (Delta324-339), block its interaction with synaptobrevin-2 (L348R), or extend the helix to promote

28 lipid-anchored syntaxin-1 and lipid-anchored synaptobrevin-2 lacking TMRs efficiently promoted sponta

29 Our present results show that synaptobrevin-2-like immunoreactivity (-LIR) is present

30 ste cells with synapses display SNAP-25- and synaptobrevin-2-like immunoreactivity (LIR).

31 Synaptobrevin-2-LIR also colocalizes with immunoreactivi

32 Synaptobrevin-2-LIR colocalizes with SNAP-25-, serotonin

33 old immunoelectron microscopy, we found that synaptobrevin-2-LIR is associated with synaptic vesicles

34 However, approximately 27% of the synaptobrevin-2-LIR taste cells do not display IP3R3-LIR

35 rom taste cells onto nerve processes express synaptobrevin-2-LIR, as well as some taste cells without

36 oth type II and type III taste cells display synaptobrevin-2-LIR.

37 All IP3R3-LIR taste cells express synaptobrevin-2-LIR.

38 Thus, synaptobrevin 2 may function in catalyzing fusion reacti

39 s as tetanus toxin did not cleave astrocytic synaptobrevin-2, nor was AA released from pure astrocyte

40 y fluorescent tagging of the vesicle protein synaptobrevin-2 or by staining with the styryl dye FM4-6

41 actor attachment protein receptor) proteins: synaptobrevin 2 (or vesicle-associated membrane protein

42 in FcepsilonRI-regulated exocytosis, whereas synaptobrevin 2- or VAMP-3-deficient mast cells did not.

43 n docking, but little effect on the rates of synaptobrevin-2 proteoliposome fusion.

44 Examining single particle fusion between synaptobrevin-2 proteoliposomes and planar-supported bil

45 Interactions between synaptobrevin 2 (Sb2) and syntaxin 1A (Sx1A) can be read

46 e-associated protein of 25 kDa (SNAP25), and synaptobrevin 2 (Sb2).

47 t the ionic layer by cuffing syntaxin 1A and synaptobrevin 2, similar to the action of SNAP25B; thus

48 s vesicle-associated membrane protein (VAMP)/synaptobrevin 2, SNAP-25, synaptophysin, or synaptotagmi

49 In the absence of synaptobrevin 2, spontaneous synaptic vesicle fusion and

50 Addition of the V(C) peptide synaptobrevin-2 (syb(57-92)) increases the docking effic

51 on of the SNARE complex by the vesicle SNARE synaptobrevin 2 (syb2) and the two plasma membrane SNARE

52 The neuronal vesicular SNARE protein synaptobrevin 2 (syb2) is anchored in the vesicle membra

53 protein attachment protein receptor (SNARE) Synaptobrevin 2 (Syb2) is known for mediating neurotrans

54 domain (TMD) of the vesicle membrane protein synaptobrevin 2 (syb2).

55 erated four variants of the synaptic v-SNARE synaptobrevin-2 (syb2) anchored to the membrane by lipid

56 tosomal-associated protein 25 (SNAP-25), and synaptobrevin-2 (Syb2).

57 complex composed of SNAP-25, syntaxin-1, and synaptobrevin-2 (sybII) proteins.

58 In contrast, the apparent number of VAMP2/synaptobrevin 2, synaptophysin, and synaptogyrin demonst

59 rotein receptor (SNARE) complex, composed of synaptobrevin 2, syntaxin and synaptosome-associated pro

60 achment protein receptors complex, including synaptobrevin 2, syntaxin, and synaptosome-associated pr

61 ent protein (SNAP) receptor (SNARE) proteins synaptobrevin 2, syntaxin-1A, and SNAP-25 is the key ste

62 SNARE complex consists of the three proteins synaptobrevin-2, syntaxin, and synaptosomal-associated p

63 f SNAP-25, and a conserved central domain of synaptobrevin-2, that exhibit a high propensity to form

64 These observations provide evidence that the synaptobrevin-2 TMD catalyzes the fusion process by its

65 , we show that conformational flexibility of synaptobrevin-2 TMD is essential for efficient Ca(2+)-tr

66 b(G76V), GFP, and a synaptic vesicle protein synaptobrevin-2 (Ub(G76V)-GFP-Syb2); (2) GFP-Syb2; or (3

67 etween two synaptic proteins syntaxin 1A and synaptobrevin 2, using an atomic force microscope and th

68 cholesterol on fusion pore formation between synaptobrevin-2 (VAMP-2)-containing proteoliposomes and

69 lex result in an additional interaction with synaptobrevin-2/VAMP2 (vesicle-associated membrane prote

70 ractions of native alpha-synuclein with both synaptobrevin-2/VAMP2 and anionic lipids.

71 In contrast, the R-SNARE protein synaptobrevin-2/VAMP2 contributes to both regulated and

72 helix 12 in Munc18 within domain 3a leads to synaptobrevin-2/VAMP2 interaction and SNARE complex form

73 n complexes composed of syntaxin-1, SNAP-25, synaptobrevin-2/VAMP2, and Munc18-1.

74 amma-synuclein was not able to interact with synaptobrevin-2/VAMP2.

75 ynuclein directly bound to the SNARE-protein synaptobrevin-2/vesicle-associated membrane protein 2 (V

76 he absence of synaptic vesicle SNARE protein synaptobrevin-2 whereas the increase in spontaneous fusi

77 uxtamembranous mutation in the SNARE-protein synaptobrevin-2, which presumably impairs force transfer