ライフサイエンスコーパス: synaptogyrin

1 mologue of a major synaptic vesicle protein, synaptogyrin.

2 rin that exhibits 47% sequence identity with synaptogyrin.

3 ences for transmission caused by the loss of synaptogyrin.

4 Synaptogyrin 1 is a synaptic vesicle protein; cellugyrin

5 erolateral thalamic nuclei, which all showed synaptogyrin 1 labeling, contained significantly less sy

6 3 compared to the ubiquitous distribution of synaptogyrin 1.

7 s associated with synaptic vesicles and that synaptogyrins 1 and 3 can reside on the same synaptic ve

8 Analysis of the relative distributions of synaptogyrins 1 and 3 in mouse brain revealed a more res

9 e-phosphorylated proteins with two neuronal (synaptogyrins 1 and 3) and one ubiquitous (cellugyrin) i

10 that control glutamate release (e.g., SV2A, synaptogyrin-1) and postsynaptic signaling (e.g., GluA1,

11 led a more restricted expression pattern for synaptogyrin 3 compared to the ubiquitous distribution o

12 naptic vesicles from mouse brain showed that synaptogyrin 3 is associated with synaptic vesicles and

13 Strong synaptogyrin 3 labeling was observed in the mossy fiber

14 situ hybridization experiments to correlate synaptogyrin 3 mRNA in cell bodies with synaptogyrin 3 p

15 late synaptogyrin 3 mRNA in cell bodies with synaptogyrin 3 protein at synapses.

16 n family, we studied the distribution of the synaptogyrin 3 protein in the nervous system.

17 rin 1 labeling, contained significantly less synaptogyrin 3.

18 urons confirmed the synaptic localization of synaptogyrin 3.

19 identify a novel interaction between DAT and synaptogyrin-3 and suggest a physical and functional lin

20 at the cytoplasmic N termini of both DAT and synaptogyrin-3 are sufficient for this interaction.

21 , we identified the synaptic vesicle protein synaptogyrin-3 as a DAT interacting protein using the sp

22 DAT and synaptogyrin-3 colocalized at presynaptic terminals from

23 Instead, the synaptogyrin-3 effect on DAT activity was abolished in t

24 Functional assays revealed that synaptogyrin-3 expression correlated with DAT activity i

25 nce for a biochemical complex involving DAT, synaptogyrin-3, and VMAT2.

26 rial creatine kinase U-type, beta-synuclein, synaptogyrin-3, synaptophysin, syntaxin 1B, synaptotagmi

27 Synaptogyrin and synaptophysin are conserved MARVEL doma

28 aptic-like microvesicles (SLMVs), along with synaptogyrin and synaptophysin.

29 closely related to synaptic vesicle protein synaptogyrin and, more remotely, to synaptophysin.

30 totagmin I, synapsin, bassoon, syntaxin, and synaptogyrin, appeared not to be associated with DA neur

31 cells demonstrated that both cellugyrin and synaptogyrin are tyrosine phosphorylated in vivo by pp60

32 Drosophila lacking synaptogyrin are viable and fertile and have no overt de

33 All of these synaptogyrins are composed of a short conserved N-termin

34 Our results suggest that synaptogyrins are conserved components of the exocytotic

35 Altogether, our data indicate that neuronal synaptogyrins are differentially expressed protein isofo

36 In rat tissues, cellugyrin and synaptogyrins are expressed in mirror image patterns.

37 Previous studies have indicated that synaptogyrins are involved in the regulation of neurotra

38 We have now identified a novel isoform of synaptogyrin called cellugyrin that exhibits 47% sequenc

39 Cellugyrin and synaptogyrin co-localize when transfected into COS cells

40 Synaptogyrins comprise a family of tyrosine-phosphorylat

41 Synaptogyrins constitute a family of synaptic vesicle pr

42 of VAMP2/synaptobrevin 2, synaptophysin, and synaptogyrin demonstrated significant intervesicle varia

43 y were severely reduced in the synaptophysin/synaptogyrin double knockout mice.

44 n synaptogyrin isoform, we now show that the synaptogyrin family in vertebrates includes two neuronal

45 We have studied the localization of synaptogyrin family members in vivo.

46 In an effort to further characterize the synaptogyrin family, we studied the distribution of the

47 represent components of signals that target synaptogyrin for endocytosis from the plasma membrane an

48 relocalize cellugyrin, a nonneuronal form of synaptogyrin, from nonsynaptic regions such as the senso

49 e proteins, we generated and characterized a synaptogyrin (gyr)-null mutant in Drosophila, whose geno

50 Our data show that synaptogyrin I and synaptophysin I perform redundant and

51 We have generated mice lacking synaptogyrin I and synaptophysin I to explore the functi

52 Moreover, expression of cellugyrin (or synaptogyrin) in PC12 cells dramatically and specificall

53 ta suggest that the synaptic vesicle protein synaptogyrin is a specialized version of a ubiquitous pr

54 Synaptogyrin is an abundant membrane protein of synaptic

55 aled that the conserved N-terminal domain of synaptogyrin is essential for inhibition, whereas the lo

56 ting of exogenously expressed cellugyrin and synaptogyrin is high.

57 Similarly, the C-terminal domain of rat synaptogyrin is necessary for localization in hippocampa

58 Furthermore, GFP-tagged rat synaptogyrin is synaptically localized in neurons of C.

59 in Drosophila, whose genome encodes a single synaptogyrin isoform and lacks a synaptophysin homolog.

60 ith the full-length structure of a new brain synaptogyrin isoform, we now show that the synaptogyrin

61 Our study suggests that the mechanisms for synaptogyrin localization are likely to be conserved fro

62 These results suggest that synaptogyrin modulates the synaptic vesicle exo-endocyti

63 ious studies in PC12 cells demonstrated that synaptogyrin or its nonneuronal paralog cellugyrin targe

64 We demonstrate that Drosophila synaptogyrin plays a modulatory role in synaptic vesicle

65 h the lowest levels in brain tissue, whereas synaptogyrin protein is only detectable in brain.

66 nsmembrane domain proteins synaptophysin and synaptogyrin represent the major constituents of synapti

67 Analysis of the regions of synaptogyrin required for inhibition revealed that the c

68 protein (GFP)-tagged Caenorhabditis elegans synaptogyrin (SNG-1) are expressed in neurons and synapt

69 localization, abundance, and conservation of synaptogyrins suggest a function in exocytosis.

70 d tyrosine phosphorylation of cellugyrin and synaptogyrins suggests a link between tyrosine phosphory

71 ocytosis from the plasma membrane and direct synaptogyrin to synaptic vesicles, respectively.

72 expressed but correlated with the amount of synaptogyrin transfected.

73 naptophysin I, which is distantly related to synaptogyrins, was also inhibitory but less active.

74 or alpha-latrotoxin, co-transfection of all synaptogyrins with hGH inhibited hGH exocytosis as stron