ライフサイエンスコーパス: synthesis

1 hysiological functions (e.g. triacylglycerol synthesis).

2 s for cholesterol and unsaturated fatty acid synthesis.

3 se in both kinetic resolution and asymmetric synthesis.

4 d descriptively and reported using narrative synthesis.

5 ) resulting in inhibition of general protein synthesis.

6 tants, disinfection of bacteria and material synthesis.

7 self-templating, and multimethod combination synthesis.

8 TD, which orchestrates various steps in mRNA synthesis.

9 s as a largely unsolved challenge in organic synthesis.

10 d proteins and it is responsible for protein synthesis.

11 emperature modifications by room-temperature synthesis.

12 ransformations allowed for completion of the synthesis.

13 l cell division by directing local cell wall synthesis.

14 ion are followed by normalization of protein synthesis.

15 mechanisms possibly involved in glycerolipid synthesis.

16 s main application has been its use in total synthesis.

17 metabolic pathways for improved biochemical synthesis.

18 d S-adenosylmethionine (dcSAM) and polyamine synthesis.

19 hrough a top-down process from simulation to synthesis.

20 nd fluorinated solvent concentrations during synthesis.

21 ling pathway that regulates synaptic protein synthesis.

22 the disparate, cytoplasmic sites of protein synthesis.

23 indicating a deficiency in C-strand fill-in synthesis.

24 mains one of the great challenges in polymer synthesis.

25 dephosphorylation and resumption of protein synthesis.

26 or histone (H3-H4)2 deposition following DNA synthesis.

27 al problems with individual steps during the synthesis.

28 crobial peptide BODIPY-cPAF26 by solid-phase synthesis (6-7 d) and its spectral and biological charac

29 to CD23 is involved in the regulation of IgE synthesis and allergen transcytosis.

30 tion fraction is a valid biomarker for liver synthesis and allows reliable prediction of mortality.

31 ce for studies on the roles of local protein synthesis and axon degeneration in ALS and can serve as

32 such as catalysis, electronics, nanomaterial synthesis and biosensing.

33 T) has a beneficial effect on muscle protein synthesis and can be augmented by protein supplementatio

34 We present the synthesis and characterization of new peptide conjugates

35 Synthesis and characterization of the mixed-valence mu-a

36 We found that 2-PAA stimulated cAMP synthesis and enhanced gap junction coupling in a concen

37 Therefore, here we report the design, synthesis and evaluation of novel GDC-0449 analogs using

38 general framework to dissect the coupling of synthesis and folding essential for riboswitch function,

39 se (LTA4H) catalyses the distal step in LTB4 synthesis and hence inhibitors of this enzyme have been

40 ditions, fructose lowers whole-body glycogen synthesis and impairs subsequent exercise performance, p

41 We introduce the synthesis and in-depth characterization of platinum(II)-

42 The review was conducted using critical synthesis and included qualitative, quantitative and mix

43 ransient ER stress, decreased global protein synthesis and increased uORF mRNA translation are follow

44 zing the enzyme and contributing to enhanced synthesis and intracellular accumulation of cholesterol.

45 requires the temporal synchronization of RNA synthesis and ligand binding-dependent conformational re

46 ase (MTHFR) gene, an enzyme essential in DNA synthesis and methylation, have been associated with sus

47 nt, we examined and observed an impact of FA synthesis and mobilization by lipolysis in somatic tissu

48 hines may have significant roles in chemical synthesis and molecular manufacturing.

49 stress associated with impaired glutathione synthesis and nuclear factor erythroid-derived 2 related

50 ns express major proteins necessary for GABA synthesis and release and that sensory neurons released

51 maintains genome fidelity by catalyzing DNA synthesis and removal of a reactive DNA repair intermedi

52 ween defects in genes that regulate ribosome synthesis and risk of CRC.

53 Advances in the synthesis and screening of small-molecule libraries have

54 plex assembly pathway conducts their initial synthesis and subsequent binding to recipient proteins.

55 ic sites ahead of nascent lagging strand DNA synthesis and subsequent bypass by error-free template s

56 P is an essential precursor for glycerolipid synthesis and the accumulation of triacylglycerol (TAG)

57 Herein, we compare the synthesis and the structural and physicochemical propert

58 e new knowledge on the genetic basis of milk synthesis and will help improve the management of the Fr

59 vity relationships (SAR) through the design, synthesis, and biological evaluation of a systematic ser

60 ace that was thought to be essential for ATP synthesis, and cannot convert gamma-subunit rotation int

61 Here we report the design, synthesis, and characterization of a set of compounds th

62 n a realistic model with facile, inexpensive synthesis, and displays many advantages over the widely

63 We report the design, synthesis, and electron spin relaxation properties of hy

64 ed mutagenesis was performed by partial gene synthesis, and enzymes were overproduced from the cold-i

65 d reduced myotube diameter, impaired protein synthesis, and increased autophagy flux in response to h

66 odeling, reduced fibroblast-derived collagen synthesis, and perturbed alignment of myofibroblast arra

67 ain can cause toxicity by inhibiting protein synthesis, and ricin B can bind to the galactose ligand

68 We report the design, synthesis, and spectroscopic characterization of a serie

69 cipating in the trend toward continuous flow synthesis, and this has led to a number of innovations i

70 sAOG, involved in ABA glycosyl ester (ABAGE) synthesis, and to a moderate induction of catabolism (Cs

71 stability, lack of immunogenicity, and easy synthesis, aptamer GR-3 against GCGR can be a promising

72 Mutations impairing flagellar synthesis are inferred to increase DegU-P, which inhibit

73 NO synthetase expression and NO synthesis are linked to altered metabolism, neoplasticit

74 time, transition metal-free, and gram-scale synthesis are the advantages of this method.

75 Allenes are useful functional groups in synthesis as a result of their inherent chemical propert

76 temperature prevented c-di-GMP-induced cAMP synthesis as well as c-di-GMP-induced stalk gene transcr

77 We report here the synthesis, assembly and cytotoxicity evaluation of self-

78 RS diets may have had depressed milk protein synthesis because these animals had decreased capacity f

79 effects of low GSH levels, we disrupted its synthesis both at birth by breeding a Gclc loxP mouse wi

80 ctions characteristic of traditional organic synthesis, but can be accomplished under environmentally

81 to reloading alone, and myofibrillar protein synthesis, but not DNA synthesis, was also elevated.

82 cate the damaged DNA, allowing stringent DNA synthesis by a replicative polymerase to resume beyond t

83 ss of telomeres replicated by lagging-strand synthesis by a yet to be defined mechanism.

84 Thus, RNA synthesis by even a single, uncoated particle can initia

85 hat miscoding antibiotics may impact protein synthesis by impairing the recognition of peptidyl-tRNA

86 ational methods have been developed to guide synthesis by predicting structures at specific compositi

87 It has been assumed that DNA synthesis by the leading- and lagging-strand polymerases

88 ia, which results from the inhibition of DNA synthesis by trapping folate cofactors in the form of 5-

89 Synthesised by a chloride anion templation strategy, the

90 s treated with clozapine show lower dopamine synthesis capacity than patients who have responded to f

91 en tested as substrates in glycosaminoglycan synthesis catalyzed by various synthases.

92 Rather, inhibition of ATP synthesis caused massive spontaneous vesicle exocytosis,

93 spread reductions in translation and protein synthesis, certain oncogenic mRNAs are spared.

94 we present an approach of cell-free protein synthesis (CFPS) that provides proteins with two differe

95 e stress response to inhibit general protein synthesis coincident with preferential translation of se

96 letion of enhancer Pol II occupancy and eRNA synthesis, concomitant with downregulation of target gen

97 The influence of synthesis conditions on electrochemical performances was

98 n in vitro, it is required for efficient DNA synthesis-coupled nucleosome assembly.

99 In this report, we describe the synthesis, development, and in vivo application of sodiu

100 that Dlx1/Dlx2 homeobox genes regulate GABA synthesis during forebrain development through direct ac

101 Suppression of rRNA synthesis during IGF-1 treatment did not prevent early i

102 ity is required to activate compensatory DNA synthesis during mitosis and to resolve mitotic interlin

103 Continuous flow synthesis enables the safe handling of diazoalkanes at e

104 e animals had decreased capacity for protein synthesis, enhanced proteolysis, inefficient energy gene

105 Here, we present the solution-phase synthesis, extensive characterization, and conformationa

106 This synthesis features biomimetic cascade reactions that inv

107 ned unchanged, suggesting divergent sites of synthesis for dominating and long-chain Dols.

108 ., the reaction of methane and CO2 to form a synthesis gas, converts two major greenhouse gases into

109 ion is not coregulated with the other biotin synthesis genes.

110 ing, paired with in situ ion gel electrolyte synthesis, gives enhanced electrode-electrolyte interfac

111 ocesses combined with graphene-nanostructure synthesis has the potential to create complex materials

112 Recent advances in chemical synthesis have yielded new nanoscale materials with prec

113 ytoplasmic transit, nuclear import, and mRNA synthesis.IMPORTANCE The fates of HIV-1 reverse transcri

114 a pectoris began not long after its original synthesis in 1847.

115 iates with polyribosomes to increase protein synthesis in a CAP-independent manner.

116 uscle and the role that ACSL6 plays in lipid synthesis in both rodent and human skeletal muscle.

117 Loss of rfaH affected LPS synthesis in both species, resulting in a shorter core o

118 ty to inhibit both proliferation and protein synthesis in patient-derived melanoma cell lines with ac

119 and photorespiratory O2 fixation, and starch synthesis in response to changes in the environment.

120 y of PGE2 to selectively autoamplify its own synthesis in stromal cells by signaling transcriptional

121 oteins are assembled into the OM after their synthesis in the cytoplasm and secretion across the inne

122 acid tagging (BONCAT) to interrogate protein synthesis in vegetative Arabidopsis (Arabidopsis thalian

123 aP is required for induction of ATF4 protein synthesis in vivo in erythroid cells during ID.

124 at ectopic dATM is sufficient to promote DNA synthesis in wild-type fat body cells.

125 Asymmetric synthesis, in which chiral organocatalysts or metal comp

126 The stereodivergent synthesis includes diastereoisomers that cannot be selec

127 p-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three o

128 To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2

129 ruption of original consolidation by protein synthesis inhibition (PSI) begun shortly after training.

130 , resulting in nanomolar potencies in an ATP synthesis inhibition assay.

131 Here, we report that specific protein synthesis inhibitors could either significantly increase

132 xtracted and narratively described variables synthesised into four categories.

133 istent with the hypothesis that Fe-S cluster synthesis is a viable target for antimicrobial developme

134 bute to this rejuvenation, but local protein synthesis is also likely.

135 single-strand nick required for error-prone synthesis is an open question.

136 host shutoff, a state in which host protein synthesis is globally inhibited.

137 The synthesis is highlighted by an enzyme-catalyzed reaction

138 , suggesting that inhibition of Fe-S cluster synthesis is the primary cause of this impairment.

139 B virus core antigen polypeptide (HBcAg/p21) synthesis, it mediates a dramatic increase in viral caps

140 ipated to find wider applications in organic synthesis, its immediate application to the construction

141 interaction with FEN1 during lagging-strand synthesis may serve to regulate sequential strand displa

142 The synthesis, metalation, and redox properties of an acycli

143 tation suppressed pah1Delta effects on lipid synthesis, nuclear/endoplasmic reticulum membrane morpho

144 this methodology was used to design a rapid synthesis of (+/-)-vindeburnol from tryptamine in only t

145 The first enantioselective total synthesis of (-)-deoxoapodine is described.

146 amethyl-1,3-dioxolane-4-carbaldehyde, facile synthesis of (-)-epi-claulansine D was accomplished via

147 The first enantioselective synthesis of (-)-himalensine A has been achieved in 22 s

148 alkaloids (-)-fasicularin 2 and a pro-forma synthesis of (-)-lepadiformine A 1.

149 periphery, a diastereo- and enantioselective synthesis of (4aR,5S,8aS)-configured decahydroquinoxalin

150 he MBH bromides has been demonstrated in the synthesis of 3,4-dihydro-2H-pyrido[1,2-a]pyrimidines.

151 s was performed, aimed to the regioselective synthesis of 3- or 5-trifluoromethylated pyrazoles.

152 Herein, we report the novel synthesis of 3D graphitic carbon networks through the py

153 We report here the synthesis of 7-phenoxy-substituted 3,4-dihydro-2H-1,2,4-

154 exsA mRNA and Sr0161 leads to a block in the synthesis of a component of the T3SS apparatus and an ef

155 In this work, we report the multicomponent synthesis of a focused histone deacetylase (HDAC) inhibi

156 cies by channeling these oxidants toward the synthesis of a functionally equivalent binuclear Mn(II)

157 Here we report the design and synthesis of a highly porous pure carbon material with m

158 re, we report an enzyme-based method for the synthesis of a library of 15 different CS oligosaccharid

159 A convenient synthesis of a library of tetrazoles through a novel and

160 Here we address the high-pressure synthesis of a new polar and antiferromagnetic corundum

161 d this technology is applied to the scalable synthesis of a polycyclic brominated natural product.

162 an optimal feedback strategy to regulate the synthesis of a protein to ensure that an event will occu

163 In this study, we report the design and synthesis of a series of diarylureas derived from PSNCBA

164 It is based on the chemical synthesis of a tetra-antennary glycan that has N-acetylg

165 l isobutyric acid has enabled the asymmetric synthesis of a wide variety of polyketides.

166 avoiding stereochemical issues enabled rapid synthesis of acrylate ketone, ester, and amide analogs.

167 We describe herein the synthesis of all C13/14 diastereomers of this spiro mole

168 synthetic route was effective for the total synthesis of all members in the homohalichondrin subgrou

169 ss to boron chemotypes, we report herein the synthesis of alpha- and beta-aminocyano(MIDA)boronates f

170 f-assembled nanoparticles, from the chemical synthesis of AMPDs, through nanoparticle preparative str

171 nsformation of the cyanide group allowed the synthesis of an advance intermediate of corresponding (+

172 g a modified Julia reaction then enabled the synthesis of an advanced synthetic intermediate containi

173 n, thus providing a convenient and efficient synthesis of an antiaromatic molecule under very mild co

174 Biomimetic synthesis of artificial enamel is a promising strategy f

175 The direct synthesis of aryl(2,4,6-trimethoxyphenyl)iodonium triflu

176 A membrane domain, thermodynamically driving synthesis of ATP.

177 While the developed asymmetric synthesis of C10 substituted anthrones is anticipated to

178 le cell synthesis of C3, rather than hepatic synthesis of C3, as the primary source of C3 driving the

179 Earlier work identified renal tubule cell synthesis of C3, rather than hepatic synthesis of C3, as

180 Our synthesis of caribenol A features the diastereoselective

181 ve revealed some 30 proteins involved in the synthesis of cellular [2Fe-2S] and [4Fe-4S] clusters and

182 nstitutes a straightforward protocol for the synthesis of certain phenyl-substituted carboxylic acids

183 A general synthesis of CF3-sulfonimidamides from sulfinamides unde

184 borylated amides are useful synthons for the synthesis of chiral beta-hydroxylated, beta-fluorinated,

185 The dominant method for the synthesis of chlorins has entailed the derivatization of

186 The synthesis of complex oligosaccharides is often hindered

187 which the former allows for rapid and robust synthesis of complex particles, and the latter can be us

188 rmation of desmoplastic reaction through the synthesis of connective tissue and extracellular matrix,

189 did SbCCR1, which could imply a role in the synthesis of defense-related lignin.

190 e formation of similar invaginations for the synthesis of dsRNA precursors of highly abundant viral a

191 eof may be promising building blocks for the synthesis of electron transport materials.

192 ion process offers a general approach to the synthesis of enantioenriched atropisomeric materials.

193 The enoyl reductase InhA, responsible for synthesis of essential mycolic acids in the mycobacteria

194 The synthesis of four nitronyl nitroxide (NN) biradicals is

195 Here, we report the synthesis of four surfactant-directed mesoporous silica

196 occurs with variable efficiencies to control synthesis of full-length and truncated SELENOP isoforms.

197 We did an evidence synthesis of health and social interventions for inclusi

198 trating the utility of this approach for the synthesis of heterofunctional glycomacromolecules.

199 The synthesis of homopropargylic alcohols under metal-free a

200 aterials, enables a modular approach for the synthesis of imidazoles.

201 ty of artificial aldolases for the practical synthesis of important chiral synthons.

202 s work, a one-pot solution method for direct synthesis of interconnected ultrafine amorphous NiFe-lay

203 ethod has a broad scope and enables both the synthesis of isolable silanes and the conversion of crud

204 We report here an online tool to accelerate synthesis of large libraries of desired mutants through

205 But, synthesis of long-chain macromolecules needed to support

206 ne (hm(5)rC) phosphoramidite for solid-phase synthesis of modified RNA oligonucleotides.

207 cess for the diastereo- and enantioselective synthesis of N-heterocycle-fused-beta-lactones from N-li

208 Enantioselective first total synthesis of naturally occurring cytotoxic (+)-subincana

209 ally affects our natural environment and the synthesis of new materials.

210 Upregulation of TSP-4 does not require the synthesis of new protein, is not caused by decreased sec

211 Herein, we demonstrate the synthesis of novel highly porous N-doped carbon nanoplat

212 ssociation during dry periods, mean that the synthesis of nucleotides and their polymerization into R

213 chromatin binding events associated with the synthesis of one RNA molecule across Pol II genes, sugge

214 ity in itself as well as a feedstock for the synthesis of other valued chemicals.

215 enously supplied fatty acids via the de novo synthesis of PA, a central metabolite for membrane phosp

216 t to take a different approach and mimic the synthesis of phospholipids using abiotic but highly sele

217 Synthesis of polyfunctionalized aminothioalkenes has bee

218 )-pyrrolidine], thus enabling the programmed synthesis of polypropionates in a fully stereocontrolled

219 -2 regulates MAPK activation, which mediates synthesis of pro-IL-1beta during inflammasome priming.

220 Activation of Nlrp3 leads to synthesis of proinflammatory cytokines and influences ep

221 oCbl, even under growth conditions where the synthesis of pseudoCbl is favored.

222 ical cycles and a promising resource for the synthesis of renewable bioproducts from atmospheric CO2

223 An efficient synthesis of S-allyl thioimidate hydrobromide salts via

224 or methyl jasmonate (MeJ) could improve the synthesis of secondary metabolites.

225 erlocked molecules has led to the design and synthesis of shuttles, switches, and machines at the nan

226 We report a low-temperature colloidal synthesis of single-layer, five-atom-thick, beta-In2Se3

227 r UV-C irradiation, whereas a more localized synthesis of stilbenes in stomata guard cells and cell w

228 A rather uniform synthesis of stilbenes is induced after UV-C irradiation

229 Synthesis of substituted beta-carbolines was accomplishe

230 s with m-CPBA is an efficient method for the synthesis of substituted bicyclo[4.3.1]deca-2,4,8-triene

231 An efficient, one-pot synthesis of substituted indenones was accomplished star

232 towards a general transition metal catalysed synthesis of tetraarylmethanes.

233 high stereoselectivity, thereby facilitating synthesis of the alkyl boronic acid drugs Velcade and Ni

234 ineleganolide, which was the first disclosed synthesis of the core of the norditerpene natural produc

235 In this work, we discuss the design and synthesis of the DiPyrO tag, characterize its effect on

236 The synthesis of the fluoranthene monomers was carried out u

237 This protocol covers the chemical synthesis of the fluorogenic amino acid Fmoc-Trp(C2-BODI

238 uent elaboration to complete the first total synthesis of the juvenimicin, M-4365, and rosamicin clas

239 Here, we provide a modern synthesis of the microbial ecology of mountain glacier e

240 Herein, we report the synthesis of the mixed-valent organometallic copper APNC

241 , the specialised polymerase responsible for synthesis of the RNA primers that are elongated by the r

242 hways are readily separated into two stages, synthesis of the seven carbon alpha, omega-dicarboxylic

243 This method was applied to a rapid synthesis of the spliceosome inhibitor herboxidiene.

244 ep process occurs in minutes and enables the synthesis of the squares [Pt4(en)4(N intersectionN)4][CF

245 -tuned cellular processes that encompass (a) synthesis of the subunits and their correct assembly and

246 rolled enantio- and diastereoselective total synthesis of the two most likely diastereomeric candidat

247 Considering the very facile synthesis of this graphene foam/TiO2 nanosheet hybrid, a

248 Our approach provides a clean gas-phase synthesis of this hitherto elusive cyclic radical specie

249 lyoxylate shunt and gluconeogenesis and into synthesis of trehalose, a disaccharide of glucose.

250 This protocol describes the synthesis of two transition metal complexes, [Ir{dF(CF3)

251 g them as biocatalysts in the chemoenzymatic synthesis of tylactone and its subsequent elaboration to

252 versatile Suzuki-Miyaura reaction toward the synthesis of very electron-poor products, making these m

253 ever, these 5' deletions greatly reduced the synthesis of viral RNA in vitro, which was detected only

254 other cells demonstrated cell-type-specific synthesis of, among others, eicosanoid-forming enzymes a

255 enome, we demonstrate that L alone initiates synthesis on naked RNA and that P serves to enhance the

256 prominence of pericyclic reactions in total synthesis, only three naturally existing enzymatic examp

257 to be essential for ornithine and polyamine synthesis, ornithine decarboxylase appeared to be the ra

258 es, and synthetic accessibility for chemical synthesis, out of 593 hits, 39 were left top hits.

259 ia glycerol production and release and lipid synthesis (particularly FFA, triglycerides, and choleste

260 Both TA polymers share a common precursor synthesis pathway, but differ in the final transfer of t

261 nvestigated from both medicinal and chemical-synthesis perspectives for decades.

262 s, we recently established a citrate-derived synthesis platform for the development of new fluorescen

263 ples impacting biological activity, detail a synthesis process yielding safe and stable compounds, an

264 Common synthesis processes yield SWNTs with large length polydi

265 plays a central role in the coordination of synthesis, processing and nuclear export of mRNAs.

266 mechanisms and may lead to better-controlled synthesis protocols.

267 Liver collagen fractional synthesis rate (FSR) and plasma lumican FSR were measure

268 would provoke a never-ending increasing mRNA synthesis rate in smaller daughter cells.

269 Protein synthesis rates are determined, at the translational lev

270 consumption and adenosine triphosphate (ATP) synthesis rates of osteosarcoma cybrid cells were measur

271 e expression levels of Adipoq and fatty acid synthesis-related genes and the circulating ADIPOQ prote

272 exploited as photoredox catalysts in organic synthesis relative to their homogeneous, molecular count

273 nd underscore the enormous complexity of 60S synthesis.Ribosome biogenesis is a dynamic process that

274 This new synthesis route can produce Criegee intermediates with a

275 The synthesis routes are categorized into the four major the

276 with no overall association observed for the synthesis score.

277 The liver is a major factor VIII (FVIII) synthesis site, and mesenchymal stem cells have been sho

278 ltransferase, a rate-limiting enzyme for NAD synthesis, specifically in the liver.

279 obtained using a one-pot microwave-assisted synthesis, starting from arsenic (III) oxide.

280 anism might selectively impact viral protein synthesis, suggesting that an NP-mediated translation st

281 of all genes involved in FA and triglyceride synthesis that are normally regulated by SREBP-1c.

282 of more highly step-economical syntheses, a synthesis that combines high levels of step economy with

283 we describe a combined biological/enzymatic synthesis that is capable of efficiently converting micr

284 bond formation has been a staple of organic synthesis, the direct transfer of primary amino (-NH2) a

285 to dsDNA with an appropriate translesion DNA synthesis (TLS) polymerase, followed by PCR amplificatio

286 In translesion DNA synthesis (TLS), specialized DNA polymerases replicate t

287 xide dismutase 2 (SOD2) in the cytosol after synthesis to transfer the enzyme to the mitochondria for

288 portance of microwave irradiation in organic synthesis today is unquestionable, but in many cases the

289 group on these modified bases prior to cDNA synthesis using enzymes.

290 Membrane synthesis was also observed as a sign of active viral re

291 myofibrillar protein synthesis, but not DNA synthesis, was also elevated.

292 Here, inspired by the Fischer indole synthesis, we report an iridium-catalysed tyrosinase-lik

293 SYNTHESIS: We identified four issues highlighting the ke

294 pathways including branched chain amino acid synthesis were significantly enriched in baseline sample

295 ic proteins and those of chloroplast protein synthesis were significantly lower in L. rostrata cells

296 in the development of complex macromolecule synthesis, where a high level of molecular precision or

297 human telomerase catalyzes processive repeat synthesis, which could restore the full 100 nucleotides

298 role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, by regul

299 is capacity by 5-35%, whereas de novo purine synthesis, which occurs in the cytosol, was not affected

300 hree enzymes, allowing for completion of the synthesis without the need for extensive screening.