ライフサイエンスコーパス: synthetic gene

1 abeled proteotypic peptides generated from a synthetic gene.

2 -His(6)-tag protein, using a codon-optimized synthetic gene.

3 ant vaccinia virus constructs expressing the synthetic gene.

4 nant human PC-TP in Escherichia coli using a synthetic gene.

5 5)N-labeled cytochrome b(5) expressed from a synthetic gene.

6 ein to high yield in Escherichia coli from a synthetic gene.

7 arkedly less stable than that encoded by the synthetic gene.

8 , LPS O-antigen synthetic genes, and capsule synthetic genes.

9 schema for standardized data description of synthetic genes.

10 s using a previously undescribed method with synthetic genes.

11 s aquaticus) to remove failure products from synthetic genes.

12 h repressive histone modifiers to inhibit BA synthetic genes.

13 dy with increased expression of the capsular synthetic genes.

14 turally occurring and nonnaturally occurring synthetic genes.

15 the expression and regulation of the starch synthetic genes.

16 and was co-expressed with most of the starch synthetic genes.

17 ific DNA endonuclease, to remove errors from synthetic genes.

18 ar use in the analysis of these genes is the synthetic gene, a nucleotide sequence designed to the sp

19 re in Escherichia coli by using an optimized synthetic gene and cold-adapted chaperonins.

20 OX was expressed in Escherichia coli using a synthetic gene and found to be a stable, monomeric, FAD-

21 y visually compare the coding sequences of a synthetic gene and its natural counterpart with an integ

22 iption terminator that is placed between the synthetic gene and reporter gene.

23 y 30-50% reduction in the expression of ATRA synthetic genes and a 120% increase in the expression of

24 d to support the design and self-assembly of synthetic genes and constructs.

25 fects on protein expression, we designed 285 synthetic genes and determined corresponding expression

26 Up-regulation of PGC-1beta and lipid synthetic genes and down-regulation of silence regulator

27 The study consists of two parts: tests with synthetic genes and experiments starting with whole LM c

28 We expressed and characterized the synthetic genes and found no significant effect of the D

29 nsively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling t

30 Expression analyses of cell-wall synthetic genes and wall-related transcription factors i

31 la pathogenicity island genes, LPS O-antigen synthetic genes, and capsule synthetic genes.

32 n Escherichia coli (200 nM culture), using a synthetic gene approach.

33 Here we show that eight synthetic genes are sufficient to promote filament assem

34 To bridge this gap a synthetic gene, ARG8m, designed to specify an arginine b

35 Next a synthetic gene array identifies nonessential genes that

36 We propose that the cobalamin synthetic genes, as well as genes providing cobalamin-de

37 e is a global down-regulation of cholesterol synthetic genes, as well as SREBP-2, in the brains of di

38 larm pheromone for many pest aphids, using a synthetic gene based on a sequence from peppermint with

39 We used a synthetic gene based on mammalian GTP cyclohydrolase I,

40 A synthetic gene based on the primary sequence of the matu

41 We designed synthetic genes based on P. falciparum, Escherichia coli

42 n of not only previously identified glycogen synthetic genes, but also a significant regulon of genes

43 iterations, we were able to reduce errors in synthetic genes by >16-fold, yielding a final error rate

44 Designing synthetic genes by hand is a time-consuming and error-pr

45 The variant GFP encoded by the synthetic gene can be expressed at a high level, forming

46 The synthetic gene can be optimized for expression and const

47 essed and purified in Escherichia coli using synthetic genes, characterized regarding biochemical pro

48 atively characterized an inducible, bistable synthetic gene circuit controlling the expression of a b

49 imple form, making them especially useful as synthetic gene circuit design equations.

50 A limiting factor in synthetic gene circuit design is the number of independe

51 titatively by single-cell data analysis of a synthetic gene circuit integrated in human kidney cells.

52 be engineered predictably using exchangeable synthetic gene circuit modules to sense and integrate mu

53 e tested different topologies and verified a synthetic gene circuit with mutual inhibition and auto-a

54 order to control transcription from a simple synthetic gene circuit.

55 Adaptability of synthetic gene circuits across different organisms could

56 Synthetic gene circuits allow the behavior of living cel

57 equire accurate predictive design of complex synthetic gene circuits and accompanying large sets of q

58 We review how CRISPR can be used to build synthetic gene circuits and discuss recent advances in C

59 Synthetic gene circuits are designed to program new biol

60 Synthetic gene circuits are emerging as a versatile mean

61 However, synthetic gene circuits are often unreliable, as changes

62 However, as synthetic gene circuits become larger and more complicat

63 ts an essential parameter in the dynamics of synthetic gene circuits but typically is not explicitly

64 This work demonstrates that synthetic gene circuits can be engineered to be robust t

65 ise a robust platform for building mammalian synthetic gene circuits capable of precisely modulating

66 illustration, we apply this strategy to two synthetic gene circuits exhibiting anomalous behaviors.

67 gration, and analysis of several large scale synthetic gene circuits for artificial tissue homeostasi

68 d our work has implications in the design of synthetic gene circuits for this purpose.

69 Recently, synthetic gene circuits have become promising tools to a

70 d TF signaling using mathematical models and synthetic gene circuits in Escherichia coli.

71 is framework, we demonstrate construction of synthetic gene circuits of up to 64 kb in size comprisin

72 The construction of synthetic gene circuits relies on our ability to enginee

73 Engineering of cell fate through synthetic gene circuits requires methods to precisely im

74 Synthetic gene circuits that combine DNA, protein, and R

75 We developed inducible synthetic gene circuits that generate varying degrees of

76 operties that can be harnessed by native and synthetic gene circuits to provide greater control over

77 le principle that adds a layer of control to synthetic gene circuits, allowing dynamic regulation of

78 l fate determination by viruses, dynamics of synthetic gene circuits, and constraints on evolutionary

79 orm for integrated logic and memory by using synthetic gene circuits, and we demonstrated the impleme

80 een made in the design and implementation of synthetic gene circuits, but real-world applications of

81 When these insulators are used to join synthetic gene circuits, the behavior of layered circuit

82 c modeling, and experimentally, using simple synthetic gene circuits.

83 ural biological networks and for engineering synthetic gene circuits.

84 sed economy through the predictive design of synthetic gene circuits.

85 for modulating and expanding the function of synthetic gene circuits.

86 control of cell metabolism, cell biology and synthetic gene circuits.

87 their components can be used for large-scale synthetic gene circuits.

88 gy has developed numerous parts for building synthetic gene circuits.

89 es a unique library of components for use in synthetic gene circuits.

90 A synthetic gene coding for a single-chain fragment (ScFv)

91 We generated a synthetic gene coding for CP4 EPSP synthase and characte

92 A synthetic gene coding for gamma-gliadin 1 (GG1) was expr

93 A bacterial expression system using a synthetic gene coding for the 215-amino acid, full-lengt

94 A synthetic gene coding for the Chlamydia trachomatis sero

95 Synthetic genes coding for 2 hybrid proteins consisting

96 Plasmids that contain synthetic genes coding for small oligoribonucleotides ca

97 The transcription factors regulate synthetic genes coding for small regulatory RNAs (called

98 ll six HCV genotypes were selected to design synthetic genes coding for this antigenic region.

99 This synthetic gene complementation approach can also be used

100 h repressors were examined in the context of synthetic gene complexes containing modular promoters an

101 A synthetic gene consisting of the reverse transcriptase (

102 With these synthetic gene constructs it was possible to demonstrate

103 A synthetic gene containing an E. coli codon bias was clon

104 farnesoid X receptor, is recruited to the BA synthetic genes Cyp7a1 and Cyp8b1 and the BA uptake tran

105 hematical function, parameterized using this synthetic gene data set, which enables computation of pr

106 Here we present the Synthetic Gene Database (SGDB): a relational database th

107 A major challenge in synthetic gene delivery is to quantitatively predict the

108 Self-assembled synthetic gene delivery systems represent the bottom-up

109 Compared with viral vectors, synthetic gene-delivery systems, such as liposomes and p

110 many modules to provide a platform for rapid synthetic gene design for multikilobase sequences.

111 e utilized to create, streamline and improve synthetic gene drives, this is rapidly changing.

112 Typically, synthetic genes encode the same product as the gene of i

113 In this study, a synthetic gene encoding a C4V3 recombinant protein, know

114 ng these chimeric antigens, we constructed a synthetic gene encoding a hybrid protein that combined b

115 In this study, a plant optimized synthetic gene encoding for the LTB-ST fusion protein ha

116 us recombination in yeast we have inserted a synthetic gene encoding human ornithine transcarbamylase

117 A synthetic gene encoding the fusion protein (Ala-Hyp)(51)

118 ian cells transfected with a codon-optimized synthetic gene encoding the KcsA protein expressed K+-se

119 A synthetic gene encoding the tandem affinity purification

120 Early tests of the hypothesis using synthetic genes encoding only clustered noncontiguous Hy

121 cts arabinosylation, we designed a series of synthetic genes encoding repetitive (Ser-Pro(2))(n), (Se

122 ples that have proven difficult to clone and synthetic genes encoding toxic products.

123 Yet design and construction of synthetic genes, especially those coding for large prote

124 Specifically, using both real and synthetic gene expression data sets, we compared the qua

125 caffolds provide a powerful way to construct synthetic gene expression programs for a wide range of a

126 The synthetic gene expression system was validated with two

127 alcohol may modulate both apoptotic and fat synthetic gene expression through homocysteine-induced E

128 A synthetic gene for chicken ovomucoid first domain (OMCHI

129 A synthetic gene for the human motilin receptor containing

130 ce microscopy of cultured cells expressing a synthetic gene for the receptor.

131 of this approach, we use it to optimize the synthetic genes for 19 repetitive proteins, and show tha

132 We constructed synthetic genes for glucagon-like peptide-1 PODs and dem

133 Synthetic genes for ten completely redesigned proteins w

134 thod of PCR-based gene synthesis, error-free synthetic genes for the human protein kinases PKB2, S6K1

135 oop i3 in G protein coupling, we constructed synthetic genes for the three main D4 receptor variants.

136 assays, plasmids containing codon-optimized synthetic gene fragments (pS plasmids) showed greater th

137 ding to a decrease in the error frequency of synthetic gene from 11.44/kb to 0.46/kb.

138 The ability to mass produce synthetic genes holds great potential for biological res

139 beled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by

140 cell, BPTI was expressed and secreted from a synthetic gene in the yeast Saccharomyces cerevisiae.

141 r-chloramphenicol acetyltransferase reporter synthetic gene in transient infection assays in neurobla

142 o code for each amino acid, and inserted the synthetic genes in DNA vaccine plasmids.

143 One problem with synthetic genes in genetically engineered organisms is t

144 the expression of IRF7-controlled natural or synthetic genes in several cell lines, including those w

145 her greatly reduce the number of full-length synthetic genes in the library.

146 otential role in the co-expression of starch synthetic genes in the maize endosperm.

147 Analysis identified increases in fatty acid synthetic genes, including Srebp-1 and fatty acid syntha

148 To meet the growing demand for synthetic genes more robust, scalable and inexpensive ge

149 We then expand our method to produce a synthetic gene network acting as a predictable timer, mo

150 We propose a synthetic gene network in Escherichia coli which combine

151 his issue by Cantone et al., who construct a synthetic gene network in yeast and use it to assess and

152 When a synthetic gene network is allowed to regulate a downstre

153 We have tested the new algorithm on the synthetic gene network library bioengineered recently.

154 The parameters in a complex synthetic gene network must be extensively tuned before

155 ize the resilience of phenotypic states in a synthetic gene network near a critical transition.

156 veloped the first mammalian mechanosensitive synthetic gene network to monitor endothelial cell shear

157 The photocaging of a synthetic gene network using unnatural amino acid mutage

158 ecific arrangement of the siRNA targets in a synthetic gene network, allow direct evaluation of any B

159 ble through the design and implementation of synthetic gene networks amenable to mathematical modelli

160 ave important implications for the design of synthetic gene networks and stress that such design must

161 Synthetic gene networks can be constructed to emulate di

162 Synthetic gene networks can be used to control gene expr

163 The ability to construct synthetic gene networks enables experimental investigati

164 Synthetic gene networks have wide-ranging uses in reprog

165 The construction of synthetic gene networks implementing simple functions ha

166 ittle over a decade ago with the creation of synthetic gene networks inspired by electrical engineeri

167 The design of synthetic gene networks requires an extensive genetic to

168 Efforts to engineer synthetic gene networks that spontaneously produce patte

169 We studied its behaviour in synthetic gene networks under increasingly complex condi

170 Synthetic gene networks will also lead to new logical fo

171 Using natural and synthetic gene networks with and without the network mot

172 of gene function and to the construction of synthetic gene networks with desired properties.

173 be able to construct useful next-generation synthetic gene networks with real-world applications in

174 ents for predicting the behavior of complex, synthetic gene networks, e.g., the whole can be differen

175 literature pertaining to the development of synthetic gene networks, the engineering framework used

176 for implementing gene expression control in synthetic gene networks.

177 genetic networks and apply it to a family of synthetic gene networks.

178 urs and also benefits the rational design of synthetic gene networks.

179 ne the design, construction and screening of synthetic gene networks.

180 netic circuit, a common motif in natural and synthetic gene networks.

181 the design, construction and modification of synthetic gene networks.

182 a useful tool for designing and controlling synthetic gene networks.

183 The organization of the fatty acid synthetic genes of Haemophilus influenzae Rd is remarkab

184 In addition, the cobalamin synthetic genes of Salmonella spp. (cob) show a regulato

185 el chromosomal integration and expression of synthetic gene operons in Synechocystis, comprising up t

186 we describe the design and construction of a synthetic gene oscillator in Escherichia coli that maint

187 Like other gene circuits, synthetic gene oscillators are noisy and exhibit fluctua

188 Synthetic gene oscillators are small, engineered genetic

189 We further demonstrate the excellence of the synthetic gene products for in vitro mapping of the nucl

190 evealed as mismatches by re-annealing of the synthetic gene products.

191 d, we have successfully constructed numerous synthetic genes, ranging from 139 to 1042 bp.

192 The ability to mass produce these synthetic genes readily will have a significant impact o

193 Analysis of synthetic gene regulatory circuits can provide insight i

194 s (TALEs) represent attractive components of synthetic gene regulatory circuits, as they can be desig

195 While control over reaction complexity using synthetic gene regulatory networks and DNA nanotechnolog

196 The ability to design and construct synthetic gene regulatory networks offers the prospect o

197 Synthetic gene regulatory networks show significant stoc

198 r, Tyr-66 to His, and Tyr-145 to Phe) with a synthetic gene sequence containing codons preferentially

199 rt the development and characterization of a synthetic gene switch that, when targeted in the mouse g

200 Synthetic gene switches, activated by pharmacologics or

201 We have constructed a synthetic gene that encodes for 12 B cell, 6 T cell prol

202 omas expressed a wide range of metabolic and synthetic genes that are expressed during logarithmic gr

203 We have produced two synthetic genes that code for the F2 domain located with

204 When synthetic genes that express RNAs for external guide seq

205 d yeast was dependent on the availability of synthetic genes that were required to improve translatio

206 ue in conjunction with specifically designed synthetic genes to identify the RS targeted by an inhibi

207 and that 5S rRNA transcripts derived from a synthetic gene transfected transiently into human cells

208 icantly improve transfection efficiencies of synthetic gene vectors.

209 The synthetic gene was cloned into a T7 promoter-controlled

210 A cluster of Bacillus subtilis fatty acid synthetic genes was isolated by complementation of an Es

211 It was reported that the starch synthetic genes were co-expressed during maize endosperm

212 glycoproteins (HRGPs) and HRGPs designed by synthetic genes were consistent with a sequence-driven c

213 uced by Escherichia coli overexpression of a synthetic gene, were reversibly unfolded in 1, 2-dimyris

214 We demonstrated that synthetic genes with tandemly arrayed tRNA-gRNA architec