ライフサイエンスコーパス: synthetic oligonucleotide

1 strand are adjustable features built into a synthetic oligonucleotide.

2 by recursive polymerase chain reaction from synthetic oligonucleotides.

3 design high-density, two-dimensional rays of synthetic oligonucleotides.

4 )-nitrosourea (BCNU) were investigated using synthetic oligonucleotides.

5 ful for studying the radical's reactivity in synthetic oligonucleotides.

6 amino group on the 5'-terminal phosphate of synthetic oligonucleotides.

7 meter to provide high-throughput analysis of synthetic oligonucleotides.

8 50 bp) were prepared using M13mp19 ssDNA and synthetic oligonucleotides.

9 non-Watson-Crick pairs have been formed with synthetic oligonucleotides.

10 y using a minimal fork substrate composed of synthetic oligonucleotides.

11 nsity arrays containing tens of thousands of synthetic oligonucleotides.

12 ycle of co-selection MAGE (CoS-MAGE) with 10 synthetic oligonucleotides.

13 ul for MS/MS-based identity test methods for synthetic oligonucleotides.

14 s are typically assembled from complementary synthetic oligonucleotides.

15 glycosylases when they are incorporated into synthetic oligonucleotides.

16 the characterization of a series of modified synthetic oligonucleotides.

17 or dsDNA analyte including PCR amplicons and synthetic oligonucleotides.

18 s protected phosphoramidite derivatives into synthetic oligonucleotides.

19 as demonstrated using an in vitro assay with synthetic oligonucleotides.

20 ty of origins, including bacteria, mice, and synthetic oligonucleotides.

21 assembly of 5- to 6-kb segments of DNA from synthetic oligonucleotides.

22 emperature (needed for the gene assembly) of synthetic oligonucleotides.

23 hanol were synthesized and incorporated into synthetic oligonucleotides.

24 Synthetic oligonucleotides (20 bases), representing all

25 Synthetic oligonucleotides (30 and 89 mers) and polymera

26 ingle-stranded calf thymus DNA (ss-ctDNA), a synthetic oligonucleotide, 5'-GATCTATGGACTTACTTCAAGGCCGG

27 se by coupling it to gene repair directed by synthetic oligonucleotides, a process in which a mutant

28 thesizing the open reading frames of L1 from synthetic oligonucleotides, altering 24% of the nucleic

29 s based on the principles of interactions of synthetic oligonucleotide analogues with mRNA.

30 by guided site-specific mutagenesis using a synthetic oligonucleotide and a plasmid carrying an indu

31 Additionally, we demonstrate that both synthetic oligonucleotides and accessible ssDNA targets

32 rotection of methylated restriction sites in synthetic oligonucleotides and can be used to measure me

33 ed product, 5-guanidino-4-nitroimidazole, in synthetic oligonucleotides and DNA treated with peroxyni

34 iodide C-nucleotides were incorporated into synthetic oligonucleotides and exposed to UV-irradiation

35 These genes were assembled by PCR from synthetic oligonucleotides and expressed in Escherichia

36 label (PCB) on the 5'-terminal phosphate of synthetic oligonucleotides and is fully compatible with

37 C element and the proximal Sp1 sites in both synthetic oligonucleotides and natural MAO B core promot

38 Using synthetic oligonucleotides and PCR techniques, each of t

39 VHCDR3 was modified using synthetic oligonucleotides and several clones with reduc

40 bridization of single base mismatch (SBM) in synthetic oligonucleotides and single-nucleotide polymor

41 nces, judged by using fluorophore-conjugated synthetic oligonucleotides and synthetic double-stranded

42 ncoding such proteins were constructed using synthetic oligonucleotides and the protein stability was

43 racts were prepared by the direct cloning of synthetic oligonucleotides, and longer runs were clones

44 We have identified two synthetic oligonucleotides (aptamers) that bind to prost

45 In addition to being important for synthetic oligonucleotides, apurinic site formation in c

46 Unpurified 40-base synthetic oligonucleotides are built into 500- to 800-bp

47 group I introns, ribozymes, RNA aptamers and synthetic oligonucleotides are included.

48 Synthetic oligonucleotides are the main cost factor for

49 Synthetic oligonucleotides are used to regulate gene exp

50 45G variant, X-ray diffraction analysis, and synthetic oligonucleotides as ligands and substrates.

51 of a variety of amine-reactive markers onto synthetic oligonucleotides as well as immobilization on

52 Saccharomyces cerevisiae based on annealing synthetic oligonucleotides at the lagging strand of DNA

53 ift assays; SirR retarded the migration of a synthetic oligonucleotide based on the Sir box in a meta

54 RNA aptamers are synthetic oligonucleotide-based affinity molecules that

55 sequence specificity not achievable by using synthetic oligonucleotide-based approaches.

56 screens and the use of both vector-based and synthetic oligonucleotide-based methods for inducing RNA

57 urately, efficiently, and rapidly from short synthetic oligonucleotide building blocks.

58 erichia coli chromosome from PCR products or synthetic oligonucleotides by homologous recombination.

59 3 vector, the vector was modified with short synthetic oligonucleotides by replacing a pelB leader se

60 Immunostimulatory CpG motifs in synthetic oligonucleotides can be effective adjuvants fo

61 a) during reverse transcription, employing a synthetic oligonucleotide consisting of a DNA primer and

62 We have detected DNA binding activity for a synthetic oligonucleotide containing an Sp1 consensus se

63 In stability tests, a synthetic oligonucleotide containing the 5-guanidino-4-n

64 Nuclear extract binding activity to a synthetic oligonucleotide containing the junB CRE-like s

65 enhanced resistance to nuclease digestion of synthetic oligonucleotides containing 4'-thio-2'-deoxynu

66 dium bromide and enhanced by the presence of synthetic oligonucleotides containing binding sites for

67 Synthetic oligonucleotides containing CpG motifs have be

68 Synthetic oligonucleotides containing CpG motifs in spec

69 Here, we show that bacterial DNA, as well as synthetic oligonucleotides containing CpG motifs, induce

70 Bacterial DNA and synthetic oligonucleotides containing CpG sequences (CpG

71 We investigated the adjuvant activity of synthetic oligonucleotides containing CpG-rich motifs li

72 Synthetic oligonucleotides containing different combinat

73 ciency involves XRCC1 or other BER proteins, synthetic oligonucleotides containing either an AP site

74 re we report that repeated administration of synthetic oligonucleotides containing foreign cytosine-p

75 Synthetic oligonucleotides containing FoU at defined sit

76 ng factor, binds tightly and specifically to synthetic oligonucleotides containing G-G base pairs (KD

77 Using synthetic oligonucleotides containing HmU at a defined s

78 res the amount of released 8-oxoguanine from synthetic oligonucleotides containing modified base in t

79 Synthetic oligonucleotides containing the lesion were pr

80 f, and recombinant TRP120 specifically bound synthetic oligonucleotides containing the motif.

81 Synthetic oligonucleotides containing the myb target sit

82 cation experiments showed that 18- and 24-bp synthetic oligonucleotides containing the RepC nick site

83 f the methionine repressor protein, MetJ, to synthetic oligonucleotides containing zero to five speci

84 nucleotides (CpG motifs) in bacterial DNA or synthetic oligonucleotides (CpG DNA) promote the product

85 availability of high-complexity libraries of synthetic oligonucleotides designed to mediate either ge

86 on was supported by gel shift analyses using synthetic oligonucleotide duplexes containing I.C or oth

87 By introducing calibrator synthetic oligonucleotides during library preparation, w

88 Synthetic oligonucleotides, each introducing one amino a

89 By ligation of synthetic oligonucleotides followed by reverse transcrip

90 de cells and thus improve our ability to use synthetic oligonucleotides for controlling cellular proc

91 ences and is retained during cleavage of the synthetic oligonucleotide from the solid support and dur

92 order to enhance DNA triple helix stability synthetic oligonucleotides have been developed that bear

93 NTNFETs with immobilized synthetic oligonucleotides have been shown to specifical

94 Synthetic oligonucleotides have been used to direct base

95 Using the technique of randomized synthetic oligonucleotide in cyclic amplification and se

96 oter region can form triplex structures with synthetic oligonucleotides in a sequence-specific manner

97 affinity purification and phosphorylation of synthetic oligonucleotides in conjunction with activated

98 e scale of A-forming tendencies exhibited by synthetic oligonucleotides in solution and the known pol

99 g of endogenous and recombinant nucleolin to synthetic oligonucleotides in which G-rich regions have

100 , it is based on the cloning of 'randomized' synthetic oligonucleotides, in which given position(s) c

101 Although efficient methods exist to assemble synthetic oligonucleotides into genes and genomes, these

102 for nucleic acid-modifying enzymes based on synthetic oligonucleotides labeled with a single fluorop

103 of an alpha-synuclein ep-PCR library and NNS synthetic oligonucleotide libraries are given as example

104 on (messenger RNA [mRNA]) using high-density synthetic oligonucleotide microarray analysis (Hu6800 Ge

105 Using synthetic oligonucleotides mimicking the three-strand fl

106 Thus, while bias in randomized synthetic oligonucleotide mixtures can be virtually elim

107 Synthetic oligonucleotides, modeled on the UAS element o

108 repeated DNA sequences generated from single synthetic oligonucleotide monomers are useful for many p

109 Using various synthetic oligonucleotide mutants, GMSAs revealed that o

110 To define the cis-acting element(s), three synthetic oligonucleotides (Na [bp -224 to -195], Nb [bp

111 Synthetic oligonucleotides (ODN) containing TTAGGG motif

112 CpG-containing sequences in bacterial DNA or synthetic oligonucleotides (ODNs) in the endosomal compa

113 studies of the CD of complexes prepared from synthetic oligonucleotides of varying length suggest tha

114 etecting large target DNA and a 50-base long synthetic oligonucleotide on the amino-modified microarr

115 Synthetic oligonucleotides (ONs) of different chemistrie

116 data base, miR145, whether transfected as a synthetic oligonucleotide or expressed from a plasmid, c

117 ences introduced to the electrode surface as synthetic oligonucleotides, PCR products, and RNA transc

118 s capable of direct RTA binding by screening synthetic oligonucleotide pools and KSHV genomic librari

119 ynthesized that enable diene modification of synthetic oligonucleotides prepared by the phosphoramidi

120 as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the

121 Bacterial 16S rDNA was amplified using 2 synthetic oligonucleotide primers specific for eubacteri

122 From a cDNA library and synthetic oligonucleotide probes based on a partial amin

123 l shift and supershift assays with different synthetic oligonucleotide probes demonstrate that protei

124 Based on in situ hybridization studies with synthetic oligonucleotide probes for vasotocin and the r

125 d strand, constructed by ligation of shorter synthetic oligonucleotides, provides layers of the DNA l

126 d was synthesized by overlap extension of 22 synthetic oligonucleotides ranging in length from 70 to

127 Synthetic oligonucleotides rarely contain 100% of the fu

128 Several short synthetic oligonucleotides, rendered resistant to mammal

129 following polymerase binding to a series of synthetic oligonucleotides representing truncations of t

130 econstitution, we found a single molecule of synthetic oligonucleotide (representing the mRNA zipcode

131 detection of low-level impurities present in synthetic oligonucleotide samples with an unprecedented

132 Synthetic oligonucleotide sequences containing Rap1p bin

133 membrane and probed with digoxigenin-labeled synthetic oligonucleotides specific for Actinobacillus a

134 Synthetic oligonucleotide strands ranging from 5 to 25 u

135 of the SuaI endonuclease was determined with synthetic oligonucleotide substrates and modified natura

136 agent on the basis of its ability to cleave synthetic oligonucleotide substrates corresponding to co

137 to psoralen interstrand crosslink-containing synthetic oligonucleotide substrates in vitro.

138 By using a series of synthetic oligonucleotide substrates, we demonstrated th

139 hage N15 and the Klebsiella phage phiKO2 and synthetic oligonucleotide substrates, we directly demons

140 Using synthetic oligonucleotide substrates, we observed that l

141 Synthetic oligonucleotides, such as oligo(dA) and oligo(

142 The assay was validated on synthetic oligonucleotide targets containing the 44 most

143 thymus DNA polymerase delta (pol delta) and synthetic oligonucleotide template-primers containing a

144 The fidelity of pol delta on synthetic oligonucleotide template-primers was compared

145 oliferating cell nuclear antigen (PCNA), and synthetic oligonucleotide template-primers.

146 Antisense oligonucleotides (ASOs) are synthetic oligonucleotides that alter expression of dise

147 Third, we report results on synthetic oligonucleotides that are tagged with a fluore

148 zed as a substrate, a result confirmed using synthetic oligonucleotides that form forked ssDNA/dsDNA

149 midite and have incorporated the analog into synthetic oligonucleotides that have been used in vitro

150 ages, including a reduced number of required synthetic oligonucleotides, the ability to analyze longe

151 ms of immunostimulation by bacterial DNA and synthetic oligonucleotides, the effects of heat shock pr

152 Using a rat C(1)-THF synthase cDNA and synthetic oligonucleotides, the rat C(1)-THF synthase ge

153 hymidine dimers and their incorporation into synthetic oligonucleotides to create vinylphosphonate in

154 We have designed synthetic oligonucleotides to give a variety of three-le

155 We used synthetic oligonucleotides to identify bases critical fo

156 we report a modular approach for conjugating synthetic oligonucleotides to proteins with aldehyde tag

157 by recursive polymerase chain reaction from synthetic oligonucleotides to rectify this problem.

158 -2 promoter can form a stable triplex with a synthetic oligonucleotide under pseudo-physiological con

159 geting and invading double-stranded DNA with synthetic oligonucleotides under physiological condition

160 The synthetic oligonucleotides used in these studies contain

161 nal DNA array which self-assembles from four synthetic oligonucleotides via a four-armed Holliday jun

162 methylation sensitivity assay, using various synthetic oligonucleotides, was used to identify the ade

163 Native and modified synthetic oligonucleotides were purified by reversed-pha

164 Synthetic oligonucleotides were used to identify a hexan

165 Synthetic oligonucleotides with a fluorescent coumarin g

166 RecA catalyzes the pairing of synthetic oligonucleotides with one strand of a double-s

167 We have built this lesion into synthetic oligonucleotides, with DHU placed at a single