ライフサイエンスコーパス: tRNA(Ala

1 AlaRSs), which remove serine from mischarged tRNA(Ala).

2 f both aminoacylation and editing domains to tRNA(Ala).

3 nome-encoded editing proteins that clear Ser-tRNA(Ala).

4 gh clearance of mischarged (with Ser or Gly) tRNA(Ala).

5 These also recognize mischarged tRNA(Ala).

6 eptor stem and/or the TPsiC loop stem of the tRNA(Ala).

7 the major determinant for identity of Dm mt tRNA(Ala).

8 acylation of the most deleterious mutants of tRNA(Ala).

9 tochondrial enzyme cannot charge cytoplasmic tRNA(Ala).

10 (37) to m(1)I(37) modification in eukaryotic tRNA(Ala).

11 inates adenosine 37 to inosine in eukaryotic tRNA(Ala).

12 a non-cognate tRNA replaced with the stem of tRNAAla.

13 utants forming substantial amounts of alanyl-tRNAAla.

14 lity of the molecule for formation of alanyl-tRNAAla.

15 could significantly amplify cellular alanyl-tRNAAla.

16 In the case of tRNAAla a single acceptor stem G.U base pair at position

17 typically recognizes the G3:U70 base pair of tRNA(Ala); a G3A change in Ashbya tRNA(Ala)UAG abolishes

18 n structure of a microhelix derived from the tRNAAla acceptor end has been determined at high precisi

19 The tRNAAla acceptor end is overall similar to A-form RNA, b

20 The anticodon stem-loop of tRNA(Ala) alone is not a functional substrate for hADAT1

21 yl-tRNA synthetase efficiently aminoacylates tRNAAla and an RNA minihelix that comprises just one dom

22 ri and Sulfolobus solfataricus hydrolyze Ser-tRNAAla and Gly-tRNAAla substrates.

23 ion into Escherichia coli EF-Tu, whereas Ala-tRNA(Ala) and Gly-tRNA(Gly) were unaffected.

24 aRS) were investigated in vivo for wild-type tRNA(Ala) and mutant tRNAs with G.U substitutions.

25 C.A, or G.A gave similar amounts of charged tRNA(Ala) and supported viability of E. coli lacking chr

26 Thus, cdc64-1 might affect charging of tRNA(Ala) and thereby initiation of cell division.

27 bicans have thus been identified: tRNA(Asp), tRNA(Ala) and tRNA(Ile).

28 Mutation of tRNA(Ala) and tRNA(Lys) had little effect on either MprF

29 ed efficiencies of tRNA(Leu(UUR)) as well as tRNA(Ala) and tRNA(Met).

30 erent amino acids; MprF1 is specific for Ala-tRNA(Ala), and MprF2 utilizes Lys-tRNA(Lys).

31 AlaRS mischarged serine and glycine to tRNA(Ala), as observed in other bacteria, and also trans

32 and in vitro deamination of adenosine 37 of tRNAala by ADAT1.

33 ecognition of mitochondrial from cytoplasmic tRNA(Ala) by translocation of G:U.

34 on aminoacylation of alanine-specific tRNA (tRNA(Ala)) by alanyl-tRNA synthetase (AlaRS) gave rise t

35 rosophila melanogaster mitochondrial (Dm mt) tRNA(Ala) contains a G:U base pair that has been translo

36 d phosphatidylglycerol (PG) catalyzed by Ala-tRNA(Ala)-dependent alanyl-phosphatidylglycerol synthase

37 ch the G3.U70 pair marks the acceptor end of tRNAAla for aminoacylation with alanine has not been cla

38 umed that the specificity for recognition of tRNA(Ala) for editing was provided by the same structura

39 ly deaminates A(37) in the anticodon loop of tRNA(Ala) from higher eukaryotes and with lower efficien

40 s assay of the expression and utilization of tRNA(ala)(GAC) also can be used to study a variety of tR

41 ssense suppression is blocked by mutation of tRNA(ala)(GAC) at a site that prevents aminoacylation by

42 Bombardment of a highly expressed dicot tRNA(ala)(GAC) gene into Zea mays bz-E2 or bz-E5 coleopt

43 an AUX/IAA gene, but rather a mutation in a tRNA(ala) gene in which the anticodon was found changed

44 ISR1 contained a tRNA(Ala) gene, while ISR2 contained a tRNA(Ile) gene.

45 wild-type and mutant versions of the Bombyx tRNAAla genes into Drosophila Schneider-2 cells and foun

46 tRNALeu genes, and 3 from three families of tRNAAla genes.

47 ted viability of E. coli lacking chromosomal tRNA(Ala) genes.

48 sophila melanogaster (Dm) mitochondrial (mt) tRNA(Ala) has a G2:U71 but not a G3:U70 pair.

49 s that mimic the amino acid acceptor stem of tRNA(Ala) has been shown, by analysis of variant minihel

50 The effect of tRNA(Ala) identity mutations on both aminoacylation effi

51 ncy between in vivo and in vitro analysis of tRNA(Ala) identity.

52 emonstrated that AlaXp deacylated mischarged tRNA(Ala) in vitro, the significance of this activity in

53 ifically and with a high efficiency on human tRNA(Ala) in vitro.

54 Moreover, DTD's activity on non-cognate Gly-tRNA(Ala) is conserved across all bacteria and eukaryote

55 nd can support growth of an Escherichia coli tRNAAla knockout strain, leading to the hypothesis that

56 produces approximately 50% reduction in the tRNA(Ala) level in mutant cells.

57 In contrast to full-length tRNAAla, minihelixAla was robustly mischarged and could

58 Docking of tRNA(Ala) on AlaRS shows critical contacts with the thre

59 e independently can provide determinants for tRNA(Ala) recognition.

60 MurNAc pentapeptide and Escherichia coli Ala-tRNAAla, respectively, and exhibited a kcat value of 660

61 iation of the key G:U base pair seen in some tRNA(Ala)s.

62 Throughout evolution, tRNA(Ala) selection by alanyl-tRNA synthetase (AlaRS) ha

63 d these differences between minihelixAla and tRNAAla, several chimeric full tRNAs were constructed.

64 itecture can efficiently edit mischarged Gly-tRNA(Ala) species four orders of magnitude more efficien

65 positively selects the universally invariant tRNA(Ala)-specific G3*U70.

66 s solfataricus hydrolyze Ser-tRNAAla and Gly-tRNAAla substrates.

67 conjunction with homoplasmic ND1 T3308C and tRNA(Ala) T5655C mutations using cybrids constructed by

68 ir in Escherichia coli alanine transfer RNA (tRNA(Ala)) that are associated with aminoacylation by al

69 hat catalyzes the transfer of l-Ala from Ala-tRNAAla to the epsilon-amino group of l-lysine of UDP-Mu

70 the particular tRNA precursor substrate for tRNA(Ala), tRNA(Val), and tRNA(His).

71 a lesser extent, differences in the in vivo tRNA(Ala):tRNA(Ser) ratio in 159 and Pn16.

72 se pair of tRNA(Ala); a G3A change in Ashbya tRNA(Ala)UAG abolishes its recognition by AgAlaRS.

73 Enzymatic studies reveal that tRNA(Ala)UAG is efficiently recognized by A. gossypii mi

74 We further demonstrate that a predicted tRNA(Ala)UAG is transcribed and accurately processed in

75 It also clears mischarged tRNAAla using a specialized domain in its C-terminal hal

76 ial recombinant fragment, targets mischarged tRNA(Ala) using a structural motif unrelated to that for

77 significance of this unique modification in tRNA(Ala), which is conserved from yeast to man.

78 tRNA(Ala) with G.C was inactive.

79 tRNA(Ala) with G.U, C.A, or G.A gave similar amounts of

80 uctures of an archaeal AlaRS in complex with tRNA(Ala) with G3*U70 and its A3*U70 variant.

81 Thus, the 3'-CCA region of tRNA(Ala) with G3*U70 is oriented to the reactive route