ライフサイエンスコーパス: tRNA(Asn)

1 ct and a reduction in steady-state levels of tRNAAsn.

2 etase to AdT, where it is converted into Asn-tRNA(Asn).

3 raordinarily high amounts of asparaginylated tRNA(Asn).

4 m the cognate products, Gln-tRNA(Gln) or Asn-tRNA(Asn).

5 (Pro), Ser-tRNA(Thr), Glu-tRNA(Gln), and Asp-tRNA(Asn).

6 r B. subtilis, and M. thermautotrophicus Glu-tRNA(Asn).

7 tRNA species, glutamyl-tRNA(Gln) or aspartyl-tRNA(Asn).

8 ischarged tRNA species, Glu-tRNA(Gln) or Asp-tRNA(Asn).

9 quire Asp-tRNA(Asn) for the synthesis of Asn-tRNA(Asn).

10 orming Asp-tRNA(Asp) but unable to recognize tRNA(Asn).

11 esis confirmed the in vitro synthesis of Asp-tRNA(Asn).

12 trast, a discriminating AspRS cannot acylate tRNA(Asn).

13 on to forming Asp-tRNA(Asp) also misacylates tRNA(Asn).

14 i unable to modify fully either tRNA(Lys) or tRNA(Asn).

15 minating one (ND-AspRS) also synthesizes Asp-tRNA(Asn), a required intermediate in protein synthesis

16 Here, we studied tRNA(Asn) accumulation as well as the catalytic capaciti

17 the genome of AsnRS and the presence of Asp-tRNA(Asn) amidotransferase, employed by the transamidati

18 pRS to Asn-tRNA by M. thermautotrophicus Asp-tRNA(Asn) amidotransferase.

19 The presence of Asp-tRNA(Asn) and ATP enhances the glutaminase activity abou

20 totrophicus GatCAB as being able to form Asn-tRNA(Asn) and Gln-tRNA(Gln), our data demonstrate that w

21 indirect path for the synthesis of both Asn-tRNA(Asn) and Gln-tRNA(Gln).

22 . trachomatis amidotransferase converted Asp-tRNA(Asn) and Glu-tRNA(Gln) into Asn-tRNA and Gln-tRNA,

23 AB) genes that modify Asp-tRNA(Asn) into Asn-tRNA(Asn) and Glu-tRNA(Gln) into Gln-tRNA(Gln); (iv) the

24 on reveals that the enzyme transamidates Asp-tRNA(Asn) and Glu-tRNA(Gln) with similar efficiency (k(c

25 hich to measure the in vivo formation of Asp-tRNA(Asn) and its acceptance by elongation factor EF-Tu.

26 l-tRNA synthetase (ND-AspRS) attaches Asp to tRNA(Asn) and the amidotransferase GatCAB transamidates

27 nating synthetases that form the misacylated tRNA(Asn) and tRNA(Gln) species.

28 wo potential overlapping codon positions for tRNA(Asn) and tRNA(Glu).

29 (Q) for guanine (G) in tRNA(His), tRNA(Asp), tRNA(Asn), and tRNA(Tyr); this changes the optimal bindi

30 AAC and UUUAAAU (underlined codon decoded by tRNA(Asn), anticodon 5' QUU 3'), frameshifting was very

31 While large amounts of Asp-tRNA(Asn) are detrimental to E. coli, smaller amounts su

32 amide aminoacyl-tRNAs, Gln-tRNA(Gln) and Asn-tRNA(Asn), are formed in many bacteria by a pretranslati

33 that Hp Asp/Glu-Adt can utilize E. coli Asp-tRNA(Asn) as a substrate.

34 o-step pathway that requires misacylated Asp-tRNA(Asn) as an intermediate.

35 ity of AdT to convert Asp-tRNA(Asn) into Asn-tRNA(Asn) by approximately 35-fold.

36 Many bacteria form Gln-tRNA(Gln) and Asn-tRNA(Asn) by conversion of the misacylated Glu-tRNA(Gln)

37 hat contain asparagine synthetase A form Asn-tRNA(Asn) by direct acylation catalyzed by asparaginyl-t

38 how that C. acetobutylicum forms Asn and Asn-tRNA(Asn) by tRNA-dependent amidation.

39 ged species, glutamyl-tRNA(Gln) and aspartyl-tRNA(Asn), by tRNA-dependent amidotransferases, as is st

40 rganized as an operon under the control of a tRNA(Asn)-dependent T-box riboswitch.

41 hetase, AdT, and Hp0100 but does not require tRNA(Asn) for assembly.

42 Most prokaryotes require Asp-tRNA(Asn) for the synthesis of Asn-tRNA(Asn).

43 s and Bacillus subtilis encode AsnRS for Asn-tRNA(Asn) formation and Asn synthetases to synthesize As

44 then subjected to in vivo selection for Asp-tRNA(Asn) formation by growth in minimal medium.

45 direct and indirect pathways of Asn and Asn-tRNA(Asn) formation.

46 are predicted to use only one route for Asn-tRNA(Asn) formation.

47 e k(cat)K(m) values of Asp-tRNA(Asp) vs. Asp-tRNA(Asn) formation.

48 gel electrophoresis showed that the residual tRNA(Asn) fraction in mutant cybrids had an altered conf

49 This complex enables direct delivery of Asp-tRNA(Asn) from the non-discriminating aspartyl-tRNA synt

50 on and no other detectable alteration in the tRNAAsn gene.

51 acterized a pathogenic mtDNA mutation in the tRNAAsn gene.

52 ntly repaired by the glutamine-dependent Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase (Asp/Glu-Adt).

53 In E. coli, therefore, hypomodification of tRNA(Asn) had little effect on frameshifting.

54 to limit the availability of asparaginylated tRNA(Asn) in the cell and, in turn, slow down the riboso

55 raensis AspRS gained the ability to form Asp-tRNA(Asn) in vitro when the W26H or K85P changes were in

56 prokaryotes synthesize asparaginyl-tRNA (Asn-tRNA(Asn)) in a similar manner using a non-discriminatin

57 Synthesis of asparaginyl-tRNA (Asn-tRNA(Asn)) in bacteria can be formed either by directly

58 S reduce this enzyme's ability to misacylate tRNA(Asn), in a manner that correlates with the toxicity

59 rase complex (Gat CAB) genes that modify Asp-tRNA(Asn) into Asn-tRNA(Asn) and Glu-tRNA(Gln) into Gln-

60 enhances the capacity of AdT to convert Asp-tRNA(Asn) into Asn-tRNA(Asn) by approximately 35-fold.

61 lso show for the first time that prokaryotic tRNA(Asn) is not inherently 'unslippery' and induces eff

62 while the enzyme is able to transamidate Asp-tRNA(Asn) (k(cat)/K(M) of 125 s(-1)/mM) it is unable to

63 ln-tRNA, by transamidation of mischarged Asp-tRNA(Asn) or Glu-tRNA(Gln) catalyzed by a heterotrimeric

64 y involves the formation of mis-acylated Asp-tRNA(Asn) or Glu-tRNA(Gln), and the subsequent amidation

65 In the aminoacylation of tRNA(Asn) or tRNA(Asp) transcripts, the mutant enzymes d

66 eads to a severe reduction in the functional tRNA(Asn) pool by increasing its in vivo degradation by

67 ites coded for Asn was due to mischarging of tRNA(Asn) rather than to codon misreading.

68 inococcus radiodurans AspRS2 is required for tRNA(Asn) recognition in vivo.

69 avored a distinct tRNA(Asn), suggesting that tRNA(Asn) recognition is not a major barrier to the rete

70 uggesting that the mutation destabilized the tRNA(Asn) secondary or tertiary structure.

71 ation of intermediate transcripts containing tRNA(Asn) sequences or decreased transcription.

72 ion of the misacylated Glu-tRNA(Gln) and Asp-tRNA(Asn) species catalyzed by the GatCAB amidotransfera

73 We detected a marked reduction in tRNA(Asn) steady-state levels which was not associated w

74 n the other hand, has not favored a distinct tRNA(Asn), suggesting that tRNA(Asn) recognition is not

75 atively involved in asparagine (Asn) and Asn-tRNA(Asn) synthesis.

76 uired in vivo for both Gln-tRNA(Gln) and Asn-tRNA(Asn) synthesis.

77 A(Asp) and the noncanonical, misacylated Asp-tRNA(Asn); this misacylated tRNA is subsequently repaire

78 M. thermautotrophicus AspRS aspartylated the tRNA(Asn) transcript.

79 be formed either by directly ligating Asn to tRNA(Asn) using an asparaginyl-tRNA synthetase (AsnRS) o

80 hat T. kodakaraensis mutant AspRSs mischarge tRNA(Asn) was also manifested in the higher level (1.7%)

81 Proof that Asp-tRNA(Asn) was generated by the methanogen synthetase was

82 Glutaminyl-tRNA(Gln) and asparaginyl-tRNA(Asn) were likely formed in LUCA by amidation of the

83 ion and 2-fold higher steady-state levels of tRNAAsn when compared with the parental mutant cell line

84 tation causes a conformational change in the tRNA(Asn) which may impair aminoacylation.

85 ating enzyme (ND-AspRS) also synthesizes Asp-tRNA(Asn), which is a required intermediate for protein

86 ase (AspRS) that acylates both tRNA(Asp) and tRNA(Asn) with aspartate.