ライフサイエンスコーパス: tRNA(Glu)

1 ; one likely to involve the transcription of tRNA(Glu) .

2 ary role is to generate Glu-tRNAGln, not Glu-tRNAGlu.

3 His-tRNA(His) and, as also seen in vivo, Glu-tRNA(Glu).

4 verlapping codon positions for tRNA(Asn) and tRNA(Glu).

5 hetase activates glutamate by ligating it to tRNA(Glu).

6 mutation did not introduce activity towards tRNA(Glu).

7 and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu).

8 ntified an HIV-1 with a PBS complementary to tRNA(Glu).

9 RlmN contacts the entire length of tRNA(Glu), accessing A37 by using an induced-fit strateg

10 ributed between the two pathways and whether tRNA(Glu) allocation limits tetrapyrrole biosynthesis an

11 ize tRNA(Met), tRNA(1,2)(Lys), tRNA(His), or tRNA(Glu), although these viruses replicate poorly.

12 a bacterial glutamyl-tRNA synthetase (GluRS):tRNA(Glu) and an archaeal leucyl-tRNA synthetase (LeuRS)

13 ting GluRS (D-GluRS) that biosynthesizes Glu-tRNA(Glu) and cannot make Glu-tRNA(Gln).

14 tRNA specificity capable of forming both Glu-tRNA(Glu) and Glu-tRNA(Gln).

15 was incubated with Escherichia coli glutamyl-tRNA(Glu) and purified recombinant Chlamydomonas reinhar

16 inert analogs that mimic substrate glutamyl-tRNA(Glu) and the glutamylated peptide intermediate, and

17 er thermautotrophicus GluRS is active toward tRNA(Glu) and the two tRNA(Gln) isoacceptors the organis

18 ting because it glutamylates both apicoplast tRNA(Glu) and tRNA(Gln), determined its kinetic paramete

19 that represent the anticodon stem loops for tRNA(Glu) and tRNA(Lys) are substrates of comparable act

20 H. pylori contains one tRNAGlu and one tRNAGln species, whereas A. ferrooxidans

21 of substrates and use either tRNAGlu or both tRNAGlu and tRNAGln.

22 ble position of the anticodons of tRNA(Lys), tRNA(Glu), and tRNA(1)(Gln).

23 5) or s(2) modification at U34 of tRNA(Lys), tRNA(Glu), and tRNA(Gln) causes ribosome pausing at the

24 role of modifications at U(34) of tRNA(Lys), tRNA(Glu), and tRNA(Gln) in maintenance of mitochondrial

25 le levels of nine tRNAs including tRNA(Lys), tRNA(Glu), and tRNA(Gln) in mto2/mss1, mto2/mto1, and mt

26 bolished modification at U(34) of tRNA(Lys), tRNA(Glu), and tRNA(Gln), caused by the combination of e

27 mnm)(5)s(2)U(34) in mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln).

28 NA synthetase from Thermus thermophilus (Glu-tRNA(Glu)) are considered.

29 osynthesis of s(2)U using unmodified E. coli tRNA(Glu) as a substrate.

30 r findings provide insight into the roles of tRNA(Glu) at the intersection of protein biosynthesis an

31 HipA only phosphorylates tRNA(Glu)-bound GltX, which is consistent with the earli

32 GatDE reducing the affinity of ND-GluRS for tRNA(Glu) by at least 13-fold.

33 RS is defective in amino acid activation and tRNA(Glu) charging.

34 tructure of the Thermus thermophilus D-GluRS:tRNA(Glu) complex, a divergent pattern of conservation i

35 ehydration is catalyzed by two proteins in a tRNA(Glu)-dependent manner.

36 r demonstrate that whereas overexpression of tRNA(Glu) does not affect tetrapyrrole biosynthesis, red

37 We estimated kcat/Km for Glu-tRNAGlu, EF-Tu and GTP forming ternary complex (T3) read

38 tobacco (Nicotiana tabacum) plants to alter tRNA(Glu) expression levels and introduced a point mutat

39 ned to use tRNA(Glu), the virus had selected tRNA(Glu) from the intracellular pool of tRNA for use in

40 One key feature that distinguishes tRNA(Glu) from tRNA(Gln) is the third position in the an

41 d in the T loop of tRNALys from Didymium and tRNAGlu from Physarum.

42 tations in nuclear genes interacting with mt-tRNAGlu including EARS2 and TRMU in the majority of affe

43 Modification of mt-tRNA(Glu) is a possible functional link between these tw

44 The chloroplast glutamyl-tRNA (tRNA(Glu)) is unique in that it has two entirely differe

45 tRNA(Gln) while rejecting the two H. pylori tRNA(Glu) isoacceptors.

46 ly, A. ferrooxidans GluRS1 glutamylated both tRNAGlu isoacceptors and the tRNA(CUG)(Gln) species.

47 hat tissue-specific mechanisms downstream of tRNA(Glu) may explain the spontaneous recovery.

48 Further characterization of HIV-1 that uses tRNA(Glu) may provide new insights into the preference f

49 nic acid (ALA), from glutamate by means of a tRNAGlu-mediated pathway.

50 e P cleavage occurred upstream of an ectopic tRNA(Glu) moiety, thereby exposing A(28), U(25)A(3), [A+

51 U is responsible for 2-thiouridylation of mt-tRNA(Glu), mt-tRNA(Lys) and mt-tRNA(Gln).

52 ylation and exacerbates the effect of the mt-tRNA(Glu) mutation by triggering a mitochondrial transla

53 ernally inherited, homoplasmic m.14674T>C mt-tRNA(Glu) mutation in 17 patients from 12 families.

54 deficiency (RIRCD), due to a homoplasmic mt-tRNA(Glu) mutation, and reversible infantile hepatopathy

55 Neither tRNA(Glu) nor tRNA(Gln) were substrates.

56 in their choice of substrates and use either tRNAGlu or both tRNAGlu and tRNAGln.

57 With a single exception (tRNA(Phe)-tRNA(Glu) pair), the parallelism is especially impressiv

58 How the tRNA(Glu) pool is distributed between the two pathways a

59 tion of GluTR activity through inhibition by tRNA(Glu) precursors causes tetrapyrrole synthesis to be

60 Corroborating this, tRNA(Glu) protected MnmA from tryptic digestion.

61 Eukaryotic tRNA(Gln) and tRNA(Glu) recognition determinants are found in equivale

62 ides in U5 complementary to the anticodon of tRNA(Glu) remained stable when grown in SupT1 cells or P

63 ms in these bacteria; for example, a site in tRNA(Glu) sequences was found to covary with tRNA(His) a

64 om a multiple sequence alignment of archaeal tRNA(Glu) sequences.

65 A and acceptor activity of the tRNA(His) and tRNA(Glu) species predicted in silico.

66 variant of the protein is cross-linked to a tRNA(Glu)substrate through the terminal methylene carbon

67 the virus was not initially designed to use tRNA(Glu), the virus had selected tRNA(Glu) from the int

68 ties that varied from -11.7 kcal/mol for Val-tRNA(Glu) to -8.1 kcal/mol for Val-tRNA(Tyr), clearly es

69 we show that this process involves glutamyl-tRNA(Glu) to activate Ser/Thr residues.

70 i, is responsible for modifying uridine13 in tRNA(Glu) to pseudouridine.

71 alyze the transfer of glutamate from charged tRNA(Glu) to the peptide substrate, or how they carry ou

72 identify a novel class of tRFs derived from tRNA(Glu), tRNA(Asp), tRNA(Gly), and tRNA(Tyr) that, upo

73 ctively cleaves a subset of tRNAs, including tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), t

74 nt with the observation that the geranylated tRNA(Glu) UUC recognizes GAG more efficiently than GAA.

75 ctify a break at the modified wobble base of tRNA(Glu(UUC)).

76 the uridine wobble base of tRNAARG(UCU) and tRNAGLU(UUC).

77 of conservation in enzymes that aminoacylate tRNA(Glu)versus those specific for tRNA(Gln) emerged and

78 -1 constructed with the PBS complementary to tRNA(Glu) was more stable than HIV-1 with the PBS comple

79 ition of glutamyl-tRNA reductase by immature tRNA(Glu) We further demonstrate that whereas overexpres

80 The H. pylori GluRS1 acylated only tRNAGlu, whereas GluRS2 was specific solely for tRNAGln.

81 GluRS) that aminoacylates both tRNA(Gln) and tRNA(Glu) with glutamate.