ライフサイエンスコーパス: tRNA(His

1 ressed by overexpressing GCN2, GCN1-GCN20 or tRNA(His).

2 h and the regular , but not to the wild-type tRNA(His).

3 vealed that evolved from yeast mitochondrial tRNA(His).

4 dependent U(-1) addition to A(73)-containing tRNA(His).

5 osine residue (G(-1)) is a unique feature of tRNA(His).

6 dition of G, and occur with tRNAs other than tRNA(His).

7 adds a G (position -1) to the 5'-terminus of tRNA(His).

8 ease in the rigorous specificity of Thg1 for tRNA(His).

9 a crucial determinant for aminoacylation of tRNA(His).

10 itions other than -1 and is not specific for tRNA(His).

11 scentus, simply lack any extra nucleotide on tRNA(His).

12 ds a single guanine to the 5' end (G(-1)) of tRNA(His).

13 NA substrates, it is absolutely specific for tRNA(His).

14 G(-1) addition to the 5' end of cytoplasmic tRNA(His).

15 rsor substrate for tRNA(Ala), tRNA(Val), and tRNA(His).

16 >C mutation alters structure and function of tRNAHis.

17 cells depleted of Thg1p lack G(-1) in their tRNAHis.

18 n proteins for addition of [alpha-32P]GTP to tRNAHis.

19 rlying the deafness-associated mitochondrial tRNAHis 12201T>C mutation.

20 on of CCA to the 3' mature sequence and, for tRNA(His), addition of a 5' G.

21 tRNA(Glu) sequences was found to covary with tRNA(His) among alphaproteobacteria.

22 ears to catalyze the activation step using p-tRNAHis and ATP.

23 eptor stem at nt 3/70 and 4/69 of Drosophila tRNA(His) and analyzed their ability to be processed by

24 lates with changes at the 3' end sequence of tRNA(His) and at many sites in histidyl-tRNA synthetase

25 rapid kinetics analysis employing mutants in tRNA(His) and its cognate aminoacyl-tRNA synthetase, the

26 rformed with these HisRS mutants and N-1:C73 tRNA(His) and microhelix(His) variants.

27 full-size tRNA and acceptor activity of the tRNA(His) and tRNA(Glu) species predicted in silico.

28 d mt genome at a unique location between the tRNA(His) and tRNA(Ser (AGY)) genes.

29 :A misreading in third codon position by His-tRNA(His) and, as also seen in vivo, Glu-tRNA(Glu).

30 G-1 nucleotide in defining the structure of tRNA(His), and to correlate structure with cognate amino

31 a mismatched G.A base pair at the 5' end of tRNA(His), and, with monophosphorylated tRNA substrates,

32 However, mutations in the tRNA(His) anticodon caused a drastic loss of in vitro hi

33 ds the guanylyate residue by recognizing the tRNA(His) anticodon.

34 major recognition elements in C. crescentus tRNA(His) are the anticodon, the discriminator base and

35 Histidine tRNAs (tRNA(His)) are unique in that they possess an extra 5'-b

36 g strand transfer with substrates that mimic tRNAHis as well as the authentic tRNA3Lys.

37 The mutation changed the conformation of tRNAHis, as suggested by slower electrophoretic mobility

38 a stable complex was formed with the cognate tRNA(His) but not with noncognate tRNA(Phe).

39 In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly

40 1) residue is required for aminoacylation of tRNA(His) by histidyl-tRNA synthetase, both in vitro and

41 ent dissociation constant (K(D)) for cognate tRNA(His) by more than 3-fold (from 3.87 to 1.17 microM)

42 proviruses containing a PBS complementary to tRNA(His) compared with that obtained by transfection of

43 A model of the Escherichia coli HisRS--tRNA(His) complex predicts an interaction between the pr

44 ue identity elements in the acceptor stem of tRNA(His) confer specificity.

45 anii lacks the G(-1) identity element on its tRNA(His), consistent with the lack of a gene encoding a

46 A loss of the first nucleotide (G(-1)) in tRNA(His) converts it to a substrate for MST1 with a K(m

47 B2(His-AC) maintained a PBS complementary to tRNA(His) for over 4 months in culture encompassing 12 s

48 tidyl-tRNA synthetase to allow efficient His-tRNA(His) formation.

49 Because tRNA(His) from archaea contains C(73), these findings ar

50 We report here that tRNA(His) from Thg1p-depleted cells is uncharged, althou

51 hg1 adds a single G residue to the 5' end of tRNA(His) (G(-1)), which serves as a crucial determinant

52 ene was initially flanked by 25 bp of the mt tRNA(His) gene at its 5' end and by 23 bp of the mt tRNA

53 tRNA(His) genes in a disjoint alphaproteobacterial group

54 Thg1p is required for tRNAHis guanylylation in vivo, because cells depleted of

55 polymerase activity of the highly conserved tRNA(His) guanylyltransferase (Thg1) enzyme, and no exam

56 The tRNA(His) guanylyltransferase (Thg1) family comprises a

57 The tRNA(His) guanylyltransferase (Thg1) is a member of a un

58 The yeast tRNA(His) guanylyltransferase (Thg1) is an essential enz

59 tRNA(His) guanylyltransferase (Thg1) post-transcriptiona

60 tRNA(His) guanylyltransferase (Thg1) specifically adds t

61 ymerases, and its members include eukaryotic tRNA(His) guanylyltransferase (Thg1), as well as Thg1-li

62 ucleotide addition reaction catalyzed by the tRNA(His) guanylyltransferase (Thg1).

63 The essential Saccharomyces cerevisiae tRNA(His) guanylyltransferase (Thg1p) is responsible for

64 Yeast tRNA(His) guanylyltransferase, Thg1, is an essential pro

65 ln(UUG)), tRNA(Pro(UGG)), tRNA(Pro(CGG)) and tRNA(His(GUG)) for Um, and tRNA(Pro(GGG)) for Am. tRNA(S

66 Mature tRNA(His) has at its 5'-terminus an extra guanylate, des

67 tRNA(His) has thus far always been found with one of the

68 A homology model of the tRNA(His)-HisRS complex was generated and used to design

69 1 with the PBS complementary to tRNA(Met) or tRNA(His); however, all of these viruses eventually reve

70 This unusual G(-1) residue is the major tRNA(His) identity element, and essential for recognitio

71 is a nearly universal feature that specifies tRNA(His) identity in all three domains of life.

72 ly 70% decrease in the steady-state level of tRNAHis in mutant cybrids, compared with control cybrids

73 for GCN1 was also reduced by overexpressing tRNA(His) in a gcn1Delta strain.

74 nthetase; the detection of antibodies to the tRNA(his) in a over a third of anti-Jo-1 sera; and the d

75 Gcn2p dependent, and to the accumulation of tRNA(His) in the nucleus.

76 Surprisingly, tRNA(His) in Thg1p-depleted cells accumulates additional

77 nificantly decreased ability to add G(-1) to tRNA(His) in vitro and significant defects in complement

78 a critical determinant for aminoacylation of tRNA(His) in vivo.

79 The additional G(-1) nucleotide on tRNA(His) is a nearly universal feature that specifies t

80 us post-transcriptional addition of G(-1) to tRNA(His) is not necessarily required.

81 quently amplification, in vivo, we note that tRNA(His) is the only stable Escherichia coli RNA with 3

82 uration reaction, which is distinct from the tRNA(His) maturation reaction typically catalyzed by Thg

83 DdiTLP2 catalyzes a mitochondria-specific tRNA(His) maturation reaction, which is distinct from th

84 opose that rather than functioning solely in tRNA(His) maturation, bacterial and archaeal TLPs are we

85 or templated nucleotide addition in archaeal tRNA(His) maturation.

86 ritical G(-1) addition reaction required for tRNA(His) maturation.

87 G-1 base of the unique G-1:C73 base pair in tRNA(His) may be to prevent end-fraying and stabilize th

88 The failure in tRNAHis metabolism was responsible for the variable redu

89 these TLPs in separate pathways unrelated to tRNA(His) metabolism, such as mitochondrial tRNA repair

90 All tRNAHis molecules are unusual in having an extra 5' GMP

91 ata provide the evidence for a mitochondrial tRNAHis mutation leading to deafness.

92 However, it was reported that tRNA(His) of a subgroup of alpha-proteobacteria, includi

93 forced to utilize tRNA(Met), tRNA(1,2)(Lys), tRNA(His), or tRNA(Glu), although these viruses replicat

94 exhibited a modest preference for the yeast tRNA(His) over the E. coli tRNA, and preferred wild-type

95 also uncovered a divergent orthogonal HisRS/tRNA(His) pair.

96 mentary to the 3'-terminal 18 nucleotides of tRNA(His) [pHXB2(His)] as well as sequences upstream of

97 In more detailed followup using the tRNA(His) precursor as the substrate, experiments to det

98 conserved D/T loop base pairs) in Drosophila tRNAHis precursors.

99 d evolved to assume its specific function in tRNA(His) processing.

100 synthetase that might be expected to affect tRNA(His) recognition, in the flipping loop, the inserti

101 rate specificity, efficiently aminoacylating tRNA(His) regardless of the presence of G(-1).

102 vitro-generated transcripts corresponding to tRNA(His) served as poor templates for Qbeta replicase;

103 ansferase step of G(-1) addition using a ppp-tRNAHis substrate, and appears to catalyze the activatio

104 but only in a templated reaction, i.e. with tRNA(His) substrates that contain a C(73) discriminator

105 f PthA4 in the maf1 mutant slightly restored tRNA(His) synthesis, indicating that PthA4 counteracts C

106 ecognition elements into an Escherichia coli tRNA(His) template, together with addition of base U20a,

107 E. coli tRNA, and preferred wild-type yeast tRNA(His) to a variant with C at the discriminator posit

108 eficiency virus type 1 (HIV-1) which utilize tRNA(His) to initiate reverse transcription [virus deriv

109 necessary adaptation by the virus for use of tRNA(His) to initiate reverse transcription.

110 aptation by the virus to efficiently utilize tRNA(His) to initiate reverse transcription.

111 ed complete histidylation of a C. crescentus tRNA(His) transcript (lacking G(-1)).

112 rescued the yeast maf1 mutant by repressing tRNA(His) transcription.

113 fication is queuosine (Q) for guanine (G) in tRNA(His), tRNA(Asp), tRNA(Asn), and tRNA(Tyr); this cha

114 teration of the 3' end of the Thg1 substrate tRNA(His) unleashes an unexpected reverse polymerase act

115 ately 60% increase in aminoacylated level of tRNAHis was observed in mutant cells.

116 no binding discrimination against mutant U73 tRNA(His) was observed, even in the presence of HSA.

117 psi C loop [GACCGAGG; pHXB2(His-T psi C)] of tRNA(His) were constructed.

118 NA synthetase (HisRS) to permit acylation of tRNA(His) with histidine.