ライフサイエンスコーパス: tRNA(Leu)

1 tRNA(Leu) charging decreased, but only small increases i

2 tRNALeu(UUR), an etiologic hot spot for such diseases, h

3 e complete initiator tRNA(Met) gene, metY; a tRNA(Leu) gene; the tpiA gene product; and the MrsA prot

4 es a single nucleotide in the anticodon of a tRNA(Leu) that changes its normal 5'CAG3' leucine antico

5 -proteobacteria, and the first instance of a tRNA(Leu)(UAA) group I intron to be found in a group of

6 thogenicity island (Pai) that is linked to a tRNA(Leu) gene found also in Pseudomonas aeruginosa but

7 The gene arrangement and lack of a tRNA(Leu(CUN)) gene in P. opilio is most parsimoniously

8 niversally conserved aspartic acid abolished tRNA(Leu) deacylation.

9 's post-transfer hydrolytic activity against tRNA(Leu) mischarged with methionine is weak.

10 itioning of G18 and G19 that is found in all tRNA(Leu); a base was inserted at position 47n between t

11 75%) reduction in the level of aminoacylated tRNA(Leu(UUR)) and a decrease in mitochondrial protein s

12 e if the decreased fraction of aminoacylated tRNA(Leu(UUR)) in A3243G mutant cells was due to a defec

13 a decrease in the fraction of aminoacylated tRNA(Leu(UUR)).

14 showed that the alteration of aminoacylation tRNA(Leu(UUR)) caused by the A3243G mutation led to mito

15 c tRNA loci (e.g., the nuclear tRNA(Gly) and tRNA(Leu), the mitochondrial tRNA(Val) and tRNA(Pro)) we

16 EGFR, but not PI3K, reduced both H3S28ph and tRNA(Leu) and 5S rRNA transcription.

17 c precursors containing the tRNA(1)(Ser) and tRNA(Leu) transcripts with a 59-nucleotide intergenic se

18 The substrate, composed of tRNA(Ser) and tRNA(Leu), is transcribed in tandem with a 59-nucleotide

19 8ph by mutant H3S28A repressed Brf1, TBP and tRNA(Leu) and 5S rRNA expression and decreased occupancy

20 process of charged and uncharged tRNALys and tRNALeu(UUR) has revealed that the separation of the two

21 nd secondary structural similarities between tRNA(Leu)UAA introns found in strains of the cyanobacter

22 The trapping of enzyme-bound tRNA(Leu) in the editing site prevents catalytic turnove

23 cantly decreased use of codons recognized by tRNA(Leu(CUN)), likely due to selection to utilize the m

24 allowing the enzyme to conditionally charge tRNA(Leu) with methionine.

25 Thus, these LeuRS mutants charge tRNA(Leu) but fail to translocate these products to the

26 fer editing activity that efficiently clears tRNA(Leu) mischarged with isoleucine.

27 e II counterparts.A minimum of six conserved tRNA(Leu) nucleotides were required to change the amino

28 either does with other known cyanobacterial tRNA(Leu)UAA introns.

29 e folding of an Escherichia coli cytoplasmic tRNALeu.

30 Reduction of Brf1 significantly decreased tRNA(Leu) and 5S rRNA transcription and repressed EGF-in

31 of tandem UAGA quadruplets by an engineered tRNA(Leu) with an eight-base anticodon loop, has been in

32 The expected tRNALeu-UUR gene was not revealed between COI and COII.

33 ermine the nucleotides that are required for tRNA(Leu) function.

34 uggested that other tRNAs can substitute for tRNA(Leu) but that interactions in addition to pairing o

35 ochondrial tRNA genes and lacks the gene for tRNA(Leu(CUN)).

36 RNA Pol III-dependent genes (Pol III genes), tRNA(Leu), tRNA(Tyr), 5S rRNA and 7SL RNA.

37 bstrate with a uridine at position 38 (human tRNA(Leu)), there was very slight formation of pseudouri

38 vivo and in vitro characteristics of type I tRNA(Leu) variants with their type II counterparts.A min

39 Experiments monitoring deacylation of Ile-tRNA(Leu) and misactivated adenylate turnover revealed t

40 quence changes, as significant levels of Ile-tRNA(Leu) are formed in the presence of high concentrati

41 In this way, a group I intron located in tRNA(Leu), which has been used extensively for phylogene

42 The T3271C mutation in tRNA(Leu(UUR)) did not affect the efficiency of aminoacy

43 Binding of gold-labeled tRNA(Leu) places leucyl-tRNA synthetase and the bifuncti

44 tation that hydrolyzes correctly charged Leu-tRNA(Leu).

45 a activity, but increased the k(cat) for Leu-tRNA(Leu) synthesis approximately 8-fold.

46 e yielded a mutant LeuRS that hydrolyzes Leu-tRNA(Leu).

47 the substrate for the condensation with Leu-tRNA(Leu) catalyzed by the C-terminal domain of DhpH.

48 that DeltaAla(P) would be condensed with Leu-tRNA(Leu).

49 wild type Leu-tRNALeu-4 (UAA) or mutant Leu-tRNALeu-4 (CUA) are each 0.4 +/- 0.2 microM.

50 to characterize the determinants of the Leu-tRNALeu-4 acceptor stem recognized by the L/F-transferas

51 een L/F-transferase and either wild type Leu-tRNALeu-4 (UAA) or mutant Leu-tRNALeu-4 (CUA) are each 0

52 urnover, thus inhibiting synthesis of leucyl-tRNA(Leu) and consequentially blocking protein synthesis

53 d an archaeal leucyl-tRNA synthetase (LeuRS):tRNA(Leu) complex.

54 RNA promoters, including that for the major tRNALeu species in Escherichia coli, tRNA1Leu.

55 Many tRNA(Leu)UAA genes from plastids contain a group I intro

56 to significantly hydrolyze misaminoacylated tRNA(Leu).

57 euRS, are required for editing of mischarged tRNALeu.

58 creased steady-state levels of mitochondrial tRNA(Leu(UUR)).

59 pathogenetic mechanism of the mitochondrial tRNA(Leu(UUR)) A3243G transition associated with the mit

60 zes a mtDNA segment within the mitochondrial tRNA(Leu(UUR)) gene immediately adjacent to and downstre

61 3G and T3271C mutations in the mitochondrial tRNA(Leu(UUR)) gene on the aminoacylation of tRNA(Leu(UU

62 ease in steady-state levels of mitochondrial tRNALeu(UUR), and a partial impairment of mitochondrial

63 n at position 3256, within the mitochondrial tRNALeu(UUR) gene in a patient with a multisystem disord

64 ated mutations are known to affect the hs mt tRNA(Leu(UUR)) gene, and the molecular-level properties

65 f the D, TPsiC, and anticodon loops of hs mt tRNA(Leu(UUR)) in the structure and function of this mol

66 uences, indicating that this region of hs mt tRNA(Leu(UUR)) is not involved in recognition by LeuRS.

67 zed nucleotides in the loop regions of hs mt tRNA(Leu(UUR)), and tRNA variants that were aminoacylate

68 rs more structured than wild-type (WT) hs mt tRNA(Leu(UUR)), indicating that the entirely AU stem of

69 The results indicate that hs mt tRNA(Leu(UUR)), which is known to have structurally weak

70 ially denatured for the wild type (WT) hs mt tRNALeu(UUR) and were significantly stabilized by mutati

71 as further elucidated with a mutant of hs mt tRNALeu(UUR) containing a stabilized D stem and a pathog

72 structure of the human mitochondrial (hs mt) tRNALeu(UUR) features several domains that are predicted

73 anscript of the A14G pathogenic mutant of mt-tRNA(Leu), which is known to dimerize, and find that the

74 high affinity wild-type and mutant human mt-tRNA(Leu(UUR)) and mt-tRNA(Lys), and stabilize mutant mt

75 ted either with the m.3243A>G mutation in mt-tRNA(Leu(UUR)) or with mutations in the mt-tRNA(Ile), bo

76 )) and mt-tRNA(Lys), and stabilize mutant mt-tRNA(Leu(UUR)).

77 ino acid residue in the presence of a mutant tRNA(Leu) molecule containing the extra nucleotide, U, a

78 by proline and that sncB69 encodes a mutant tRNA(Leu) that corrects the mutation.

79 Native A3243G mutant tRNA(Leu(UUR)) was 25-fold less efficiently aminoacylate

80 tion efficiencies among wild-type and mutant tRNA(Leu(UUR)) transcripts.

81 noacylation kinetics of wild-type and mutant tRNA(Leu(UUR)), using both native and in vitro transcrib

82 show a decrease in the cellular abundance of tRNA(Leu).

83 ions, including introns and the anticodon of tRNA(Leu).

84 When the 3' end of tRNA(Leu) binds to the editing active site, the boron cr

85 cer cells (MCF-7) decreases the induction of tRNA(Leu) and 5S rRNA genes by alcohol, whereas reductio

86 equence alters mitochondrial localization of tRNA(Leu), and in vivo studies also show a decrease in t

87 lation is facilitated by the misacylation of tRNA(Leu) with methionine by the methionyl-tRNA syntheta

88 ing function to correct misaminoacylation of tRNA(Leu) by isoleucine and methionine.

89 The rate of misaminoacylation of tRNA(Leu) by isoleucine and valine increases with the in

90 st enough to completely block mischarging of tRNA(Leu), resulting in codon ambiguity and statistical

91 ced occupancy of H3S28ph in the promoters of tRNA(Leu) and 5S rRNA.

92 The decoding properties of tRNA(Leu) with U at position 33.5 of its eight-membered

93 tRNA(Leu(UUR)) gene on the aminoacylation of tRNA(Leu(UUR)).

94 by reduced the aminoacylated efficiencies of tRNA(Leu(UUR)) as well as tRNA(Ala) and tRNA(Met).

95 ate levels and the aminoacylated fraction of tRNA(Leu(UUR)) are likely to contribute to the decreases

96 rase-dependent increase in the proportion of tRNA(Leu(CAA)) containing m(5)C at the wobble position,

97 ichia coli LeuRS abolished aminoacylation of tRNALeu and also amino acid editing of mischarged tRNA m

98 Mutations in the anticodon and extra arm of tRNALeu-1 do not measurably effect its ability to serve

99 es of tRNASer genes, 7 from five families of tRNALeu genes, and 3 from three families of tRNAAla gene

100 leucine occurs through misaminoacylation of tRNALeu, similar to the misincorporation of norleucine f

101 Complementary sequencing of tRNALeu(UUR)has allowed the localization of this modific

102 n in the occupancy of all TFIIIB subunits on tRNA(Leu) genes, whereas prolonged PTEN expression resul

103 TM84 requires tRNA(Leu) for tight binding to the LeuRS synthetic activ

104 d containing three tRNA genes, tRNA(1)(Ser), tRNA(Leu), and tRNA(2)(Ser).

105 nd interacts with the corner of the L-shaped tRNALeu.

106 e is imported rapidly, while the mature-size tRNA(Leu) fails to be imported in this system.

107 ue to selection to utilize the more specific tRNA(Leu(UUR)) anticodon.

108 cyl-tRNA synthetase by formation of a stable tRNA(Leu)-AN2690 adduct in the editing site of the enzym

109 constant of 22 nM for one of its substrates, tRNA(Leu)(CAG).

110 aled differences in the contributions of the tRNA(Leu) acceptor stem base-pairs to tRNA(Leu) function

111 ficity was contingent on the presence of the tRNA(Leu) acceptor stem sequence.

112 found to interrupt the anticodon loop of the tRNA(Leu)(UAA) gene in a bacterium belonging to the gamm

113 Extra copies of the tRNA(Leu)(UAG) gene rescued the cold sensitivity and in

114 tide linker and allows interactions with the tRNA(Leu) elbow.

115 ions of the mutation at position 3243 in the tRNA(Leu(UUR)) gene associated with the MELAS encephalom

116 ue, the levels of three point mutations, the tRNA(Leu(UUA)) 3243 mutation causing mitochondrial encep

117 which probably destroyed the function of the tRNA(Leu(CUN)) gene.

118 ribosomes, possibly as a consequence of the tRNA(Leu(UUR)) aminoacylation defect.

119 ion was an inefficient aminoacylation of the tRNA(Leu(UUR)).

120 The phylogenetic distribution of the tRNA(Leu)UAA intron follows the clustering of rRNA seque

121 In particular, the tRNA(Leu(UUR)) A3243G mutation causes mitochondrial ence

122 Our data support the notion that the tRNA(Leu)UAA intron was inherited by cyanobacteria and p

123 ing analysis of the mtDNA segment within the tRNA(Leu(UUR)) gene that binds the transcription termina

124 ription termination region (TERM) within the tRNA(Leu(UUR)) gene was consistently and strongly protec

125 limiting C-terminal domain accessibility to tRNA(Leu) facilitates its role in protein synthesis and

126 of the tRNA(Leu) acceptor stem base-pairs to tRNA(Leu) function: in the type I, but not the type II f

127 in the amount of ND1 mRNA and co-transcribed tRNA(Leu(UUR)) in mutant cells.

128 , using both native and in vitro transcribed tRNA(Leu(UUR)).

129 lated in vitro, compared to native wild-type tRNA(Leu(UUR)).

130 n pattern was observed between the wild-type tRNALeu(UUR)and its counterpart carrying the A3243G muta

131 and processing of the genes tRNA(Thr)(UGU), tRNA(Leu)(UAA), and tRNA(Phe) (GAA) therefore attributes

132 induce H3S28ph, which, in turn, upregulates tRNA(Leu) and 5S rRNA transcription through Brf1 and TBP

133 Using tRNA(Leu) purified from a DUS 2 knockout strain of yeast

134 .5-7.3 kb of dissimilar intervening DNA with tRNA(Leu)-queA-tgt sequences that are also found in Pseu

135 hat the association of BRF1 and pol III with tRNA(Leu) genes in cells decreases when ERK is inactivat