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1 ndrially encoded transfer RNA methionine (mt-tRNA(Met)).
2 slation at this AUG codon by using initiator tRNAmet.
3 ments including those derived from initiator-tRNA(Met).
4 s of tRNA(Leu(UUR)) as well as tRNA(Ala) and tRNA(Met).
5 at gives rise to the initiator and elongator tRNA(Met).
6 in vitro transcript of bovine mitochondrial tRNA(Met).
7 methionine by the one mitochondrial-encoded tRNA(Met).
8 s the Met surrogate azidonorleucine (Anl) to tRNA(Met).
9 terize the virus with a PBS complementary to tRNA(Met), a DNA fragment encompassing the PBS and U5 re
10 er mutants maintained a PBS complementary to tRNAMet after in vitro culture, although all contained m
12 We report that RPUSD4 binds 16S mt-rRNA, mt-tRNA(Met), and mt-tRNA(Phe), and we demonstrate that it
13 print) analysis with 30 S ribosoma subunits, tRNAMet, and a model segment of geneX-secA RNA carrying
14 U5 complementary to the anticodon region of tRNA(Met); and pHXB2(Met-C-Met), which contains two G-to
16 orm on a hybrid mRNA construct consisting of tRNA(Met) attached at the 5'-end of a mitochondrial prot
17 loop-containing tRNASer(UCN), tRNASer(AGN), tRNAMet(AUA), tRNATrp, and tRNAPro genes occur in M. cal
18 d cells involves the inhibition of initiator tRNA(Met) binding to eukaryotic translation initiation f
20 the second maintained a PBS complementary to tRNA(Met) but contained a 26-nucleotide deletion in U5 u
21 Formylation of initiator methionyl-tRNA (Met-tRNA(Met)) by methionyl-tRNA formyltransferase (MTF) is
22 evisiae in the absence of eIF2 and initiator tRNA(met), by the same mechanism of factor-independent r
26 et) stably maintained a PBS complementary to tRNA(Met) during the 125-day culture period examined.
27 e-tRNA(Phe), an analog of the initiator fMet-tRNA(Met), enhanced the population of complexes that cou
34 alian mitochondrial genome contains a single tRNA(Met) gene that gives rise to the initiator and elon
35 usA operon, including the complete initiator tRNA(Met) gene, metY; a tRNA(Leu) gene; the tpiA gene pr
37 (T4409C) in the gene for human mitochondrial tRNA(Met) (hmtRNA(Met)) has been found to cause mitochon
38 ibosomal subunits, eIFs 3, 2, 1, and 1A, and tRNA(Met)(i) attach to the 5'-proximal region of mRNA an
40 fficient eIF2-independent recruitment of Met-tRNA(Met)(i) to 40S/mRNA complexes, if attachment of 40S
43 cooperation with eIF3, eIF1, and eIF1A, Met-tRNA(Met)(i)/eIF2/GTP binds to 40S subunits yielding 43S
44 n tRNA that is critical for the stability of tRNA(Met)i The crystal structure of the homotetrameric m
45 bility of the yeast m1A58 tRNA Mtase to bind tRNA(Met)i, indicating that each subunit contributes to
46 nspliced precursor RNAs of dimeric tRNA(Ser)-tRNA(Met)i, suggesting a novel nuclear role for RNase MR
47 mino acid azidonorleucine (Anl) to elongator tRNA(Met) in hamster (CHO), monkey (COS7), and human (He
50 the U5-PBS structure and disrupt the U5-PBS-tRNAMet interaction of the virus derived from HXB2(Met-A
53 compassing regions in U5 which interact with tRNAMet, maintained a PBS complementary to tRNAMet follo
55 BS) viral RNA genome alone or complexed with tRNAMet of HXB2(Met-AC) revealed structural motifs in co
56 ographic behavior of elongator and initiator tRNAMet on a RPC-5 column indicated that both species ar
57 -tRNA, using either the native mitochondrial tRNA(Met) or an in vitro transcript of bovine mitochondr
58 ble than HIV-1 with the PBS complementary to tRNA(Met) or tRNA(His); however, all of these viruses ev
59 yl-tRNA analogues N-Ac-Phe-tRNAPhe, N-Ac-Met-tRNAMet or f-Met-tRNAfMet with puromycin, conditions fav
60 th pHXB2 except for the PBS complementary to tRNA(Met); pHXB2(Met-AC-Met), which contains the PBS seq
61 A novel HIV-1 mutant which stably utilizes tRNAMet rather than tRNA3Lys as a primer was previously
62 , we demonstrate that m(5)C deficiency in mt-tRNA(Met) results in the lack of 5-formylcytosine (f(5)C
63 ed to destabilize contact regions of U5 with tRNAMet, reverted back to contain a PBS complementary to
64 a PBS complementary to the 3' nucleotides of tRNA(Met); the PBS of this virus was not stable upon ext
65 We have used this fragmented P-site-bound tRNA(Met) to identify two 2'-hydroxyl groups at position
67 c protein that transfers methionyl-initiator tRNA(Met) to the small ribosomal subunit in a ternary co
68 Lys) as the primer, can be forced to utilize tRNA(Met), tRNA(1,2)(Lys), tRNA(His), or tRNA(Glu), alth
70 crimination against wheat germ initiator Met-tRNAMet was almost entirely due to the 2'-phosphoribosyl
71 f the first (1.72) base pair of tRNAGln, and tRNAMet was proposed by others to have a similar base-pa
72 as a whole, since some cytoplasmic RNAs like tRNA(Met) were vastly underrepresented, while U6 spliceo
73 t on processing or accumulation of elongator tRNAMet, which also contains m1A at position 58, suggest
74 the ribosome can translocate a P-site-bound tRNA(Met) with a break in the phosphodiester backbone be
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